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allergic bronchopulmonary mycosis due to exposure to eurotium herbariorum after the great east japan earthquake.indoor mold levels typically increase after natural disasters, flooding, and water damage. eurotium herbariorum is the sexual stage of aspergillus glaucus. case presentation a 66-year-old, japanese male, ex-smoker had been diagnosed with bronchial asthma when he was five years old; he achieved remission at the age of 13 years. he was displaced from his home during the great east japan earthquake on march 11, 2011 and moved to temporary housing in miyagi prefecture in june 2011. he experienced th ...201728807087
the bacterial species associated with aspirated foreign bodies in children.inhaled foreign bodies in children are common and may be complicated by secondary airway tract infection. the inhaled foreign body may act as carrier of infectious material and the aim of this study was to explore the bacterial species associated with aspirated foreign bodies in a cohort of children.201728779997
transcription factor agseb1 affects development, osmotic stress response, and secondary metabolism in marine-derived aspergillus glaucus.fungi possess sophisticated regulatory systems to respond to a vast array of environmental signals. among these responsive networks, some genes play critical roles in the regulation of various cellular processes. here, we identified a putative transcriptional factor agseb1 in aspergillus glaucus, a marine-derived filamentous fungus. agseb1 encodes a protein with two c2 h2 zinc fingers at the c-terminus, similar to the placement of these motifs in msn2/4 of saccharomyces cerevisia, where they are ...201728745839
biomineralisation of fipronil and its major metabolite, fipronil sulfone, by aspergillus glaucus strain ajag1 with enzymes studies and bioformulation.fipronil is a phenylpryazole insecticide which is extensively used for the protection of agricultural yields. however, this insecticide poses various threats to  the environment. therefore it is essential to develop an effective method to degrade or eliminate this pollutant from the  environment. in this present study, a fungal strain ajag1 capable of degrading fipronil and its metabolite, fipronil sulfone, was isolated through enrichment technique. isolated fungal strain was identified as asper ...201728667652
potential of submergedly cultivated mycelia of ganoderma spp. as antioxidant and antimicrobial agents.the study aimed to evaluate the antiradical and antimicrobial (antibacterial and antifungal) potentials of ethanol mycelial extracts of selected ganoderma species and strains and to define interand intraspecies diversity among ganoderma species and strains. ganoderma lucidum strains were good dpph• scavengers (neutralizing up to 57.12% radicals), contrary to g. applanatum (20.35%) and g. carnosum (17.04%). high correlations between the activities and contents of total phenols in the extracts sho ...201626420047
potential of submergedly cultivated mycelia of ganoderma spp. as antioxidant and antimicrobial agents.the study aimed to evaluate the antiradical and antimicrobial (antibacterial and antifungal) potentials of ethanol mycelial extracts of selected ganoderma species and strains and to define interand intraspecies diversity among ganoderma species and strains. ganoderma lucidum strains were good dpph• scavengers (neutralizing up to 57.12% radicals), contrary to g. applanatum (20.35%) and g. carnosum (17.04%). high correlations between the activities and contents of total phenols in the extracts sho ...201626420047
production, purification, and characterization of a novel cold-active superoxide dismutase from the antarctic strain aspergillus glaucus 363.the antarctic fungal strain aspergillus glaucus 363 produces cold-active (ca) cu/zn-superoxide dismutase (sod). the strain contains at least one gene encoding cu/zn-sod that exhibited high homology with the corresponding gene of other aspergillus species. to our knowledge, this is the first nucleotide sequence of a ca cu/zn-sod gene in fungi. an effective laboratory technology for a. glaucus sod production in 3 l bioreactors was developed on the basis of transient cold-shock treatment. the tempe ...201627109365
production, purification, and characterization of a novel cold-active superoxide dismutase from the antarctic strain aspergillus glaucus 363.the antarctic fungal strain aspergillus glaucus 363 produces cold-active (ca) cu/zn-superoxide dismutase (sod). the strain contains at least one gene encoding cu/zn-sod that exhibited high homology with the corresponding gene of other aspergillus species. to our knowledge, this is the first nucleotide sequence of a ca cu/zn-sod gene in fungi. an effective laboratory technology for a. glaucus sod production in 3 l bioreactors was developed on the basis of transient cold-shock treatment. the tempe ...201627109365
aspergillus glaucus aquaglyceroporin gene glpf confers high osmosis tolerance in heterologous organisms.aquaglyceroporins (glpfs) that transport glycerol along with water and other uncharged solutes are involved in osmoregulation in myriad species. fungal species form a large group of eukaryotic organisms, and their glpfs may be diverse, exhibiting various activities. however, few filamentous fungal glpfs have been biologically investigated. here, a glpf gene from the halophilic fungus aspergillus glaucus (agglpf) was verified to be a channel of water or glycerol in xenopus laevis oocytes and was ...201526209670
aspergillus glaucus aquaglyceroporin gene glpf confers high osmosis tolerance in heterologous organisms.aquaglyceroporins (glpfs) that transport glycerol along with water and other uncharged solutes are involved in osmoregulation in myriad species. fungal species form a large group of eukaryotic organisms, and their glpfs may be diverse, exhibiting various activities. however, few filamentous fungal glpfs have been biologically investigated. here, a glpf gene from the halophilic fungus aspergillus glaucus (agglpf) was verified to be a channel of water or glycerol in xenopus laevis oocytes and was ...201526209670
[inhibition of aflatoxin production and fungal growth on stored corn by allyl isothiocyanate vapor].studies were conducted to determine the effectiveness of allyl isothiocyanate (ait) vapor treatment with a commercial mustard seed extract (wasaouro(®)) in controlling aflatoxin-producing fungi on stored corn. the concentration of ait in the closed container peaked at 54.6 ng/ml on the 14th day and remained at 21.8 ng/ml on the 42nd day. ait inhibited visible growth of aflatoxigenic molds in unsterilized corn and in sterilized corn inoculated with various aflatoxigenic fungi. however, fungi such ...201525748979
[inhibition of aflatoxin production and fungal growth on stored corn by allyl isothiocyanate vapor].studies were conducted to determine the effectiveness of allyl isothiocyanate (ait) vapor treatment with a commercial mustard seed extract (wasaouro(®)) in controlling aflatoxin-producing fungi on stored corn. the concentration of ait in the closed container peaked at 54.6 ng/ml on the 14th day and remained at 21.8 ng/ml on the 42nd day. ait inhibited visible growth of aflatoxigenic molds in unsterilized corn and in sterilized corn inoculated with various aflatoxigenic fungi. however, fungi such ...201525748979
prevention of toxigenic fungal growth in stored grains by carbon dioxide detection.the growth of toxigenic fungi can adversely affect grain quality and even produce mycotoxins of food safety concern, which should be sensitively monitored and controlled during grain storage. to establish the relationship between the growth of toxigenic fungi and their carbon dioxide (co2) production, the pattern of co2 concentration changes was studied during the fungal growth in grain. the results showed the co2 concentrations increased exponentially (r ≥ 0.96) during the growth of toxigenic f ...201525254604
prevention of toxigenic fungal growth in stored grains by carbon dioxide detection.the growth of toxigenic fungi can adversely affect grain quality and even produce mycotoxins of food safety concern, which should be sensitively monitored and controlled during grain storage. to establish the relationship between the growth of toxigenic fungi and their carbon dioxide (co2) production, the pattern of co2 concentration changes was studied during the fungal growth in grain. the results showed the co2 concentrations increased exponentially (r ≥ 0.96) during the growth of toxigenic f ...201525254604
a ribosomal protein agrps3ae from halophilic aspergillus glaucus confers salt tolerance in heterologous organisms.high salt in soils is one of the abiotic stresses that significantly reduces crop yield, although saline lands are considered potential resources arable for agriculture. currently, genetic engineering for enhancing salt tolerance is being tested as an efficient and viable strategy for crop improvement. we previously characterized a large subunit of the ribosomal protein rpl44, which is involved in osmotic stress in the extremely halophilic fungus aspergillus glaucus. here, we screened another ri ...201525642759
a ribosomal protein agrps3ae from halophilic aspergillus glaucus confers salt tolerance in heterologous organisms.high salt in soils is one of the abiotic stresses that significantly reduces crop yield, although saline lands are considered potential resources arable for agriculture. currently, genetic engineering for enhancing salt tolerance is being tested as an efficient and viable strategy for crop improvement. we previously characterized a large subunit of the ribosomal protein rpl44, which is involved in osmotic stress in the extremely halophilic fungus aspergillus glaucus. here, we screened another ri ...201525642759
isolation of salt stress-related genes from aspergillus glaucus ccha by random overexpression in escherichia coli.the halotolerant fungus aspergillus glaucus ccha was isolated from the surface of wild vegetation around a saltern with the salinity range being 0-31%. here, a full-length cdna library of a. glaucus under salt stress was constructed to identify genes related to salt tolerance, and one hundred clones were randomly selected for sequencing and bioinformatics analysis. among these, 82 putative sequences were functionally annotated as being involved in signal transduction, osmolyte synthesis and tran ...201425383373
isolation of salt stress-related genes from aspergillus glaucus ccha by random overexpression in escherichia coli.the halotolerant fungus aspergillus glaucus ccha was isolated from the surface of wild vegetation around a saltern with the salinity range being 0-31%. here, a full-length cdna library of a. glaucus under salt stress was constructed to identify genes related to salt tolerance, and one hundred clones were randomly selected for sequencing and bioinformatics analysis. among these, 82 putative sequences were functionally annotated as being involved in signal transduction, osmolyte synthesis and tran ...201425383373
synthesis and fungistatic activity of bicyclic lactones and lactams against botrytis cinerea, penicillium citrinum, and aspergillus glaucus.six analogues of natural trans-4-butyl-cis-3-oxabicyclo[4.3.0]nonan-2-one (3) and three derivatives, 11, 12, and 13, of vince lactam (10) were synthesized and tested as fungistatic agents against botrytis cinerea am235, penicillium citrinum am354, and six strains of aspergillus. moreover, bioresolution carried out by means of whole cell microorganisms and commercially available enzymes afforded opposite enantiomerically enriched (-) and (+) isomers of vince lactam (10), respectively. the effect ...201425110806
synthesis and fungistatic activity of bicyclic lactones and lactams against botrytis cinerea, penicillium citrinum, and aspergillus glaucus.six analogues of natural trans-4-butyl-cis-3-oxabicyclo[4.3.0]nonan-2-one (3) and three derivatives, 11, 12, and 13, of vince lactam (10) were synthesized and tested as fungistatic agents against botrytis cinerea am235, penicillium citrinum am354, and six strains of aspergillus. moreover, bioresolution carried out by means of whole cell microorganisms and commercially available enzymes afforded opposite enantiomerically enriched (-) and (+) isomers of vince lactam (10), respectively. the effect ...201425110806
genome shuffling of aspergillus glaucus hgz-2 for enhanced cellulase production.the production of cellulase from aspergillus glaucus hgz-2 was improved by using genome shuffling. the starting populations, obtained by uv irradiation, were subjected to recursive protoplast fusion. the optimal conditions for protoplast formation and regeneration were 7 mg/ml snailase and 5 mg/ml cellulase at 34 °c for 3.0 h using 0.7 m nacl as an osmotic stabilizer. the protoplasts were inactivated under uv for 30 min or heated at 50 °c for 50 min, and a fusant probability of about 100 % was o ...201425099375
genome shuffling of aspergillus glaucus hgz-2 for enhanced cellulase production.the production of cellulase from aspergillus glaucus hgz-2 was improved by using genome shuffling. the starting populations, obtained by uv irradiation, were subjected to recursive protoplast fusion. the optimal conditions for protoplast formation and regeneration were 7 mg/ml snailase and 5 mg/ml cellulase at 34 °c for 3.0 h using 0.7 m nacl as an osmotic stabilizer. the protoplasts were inactivated under uv for 30 min or heated at 50 °c for 50 min, and a fusant probability of about 100 % was o ...201425099375
genetically shaping morphology of the filamentous fungus aspergillus glaucus for production of antitumor polyketide aspergiolide a.for filamentous fungi, the basic growth unit of hyphae usually makes it sensitive to shear stress which is generated from mechanical force and dynamic fluid in bioreactor, and it severely decreases microbial productions. the conventional strategies against shear-sensitive conundrum in fungal fermentation usually focus on adapting agitation, impeller type and bioreactor configuration, which brings high cost and tough work in industry. this study aims to genetically shape shear resistant morpholog ...201424886193
genetically shaping morphology of the filamentous fungus aspergillus glaucus for production of antitumor polyketide aspergiolide a.for filamentous fungi, the basic growth unit of hyphae usually makes it sensitive to shear stress which is generated from mechanical force and dynamic fluid in bioreactor, and it severely decreases microbial productions. the conventional strategies against shear-sensitive conundrum in fungal fermentation usually focus on adapting agitation, impeller type and bioreactor configuration, which brings high cost and tough work in industry. this study aims to genetically shape shear resistant morpholog ...201424886193
abiotic stress resistance, a novel moonlighting function of ribosomal protein rpl44 in the halophilic fungus aspergillus glaucus.ribosomal proteins are highly conserved components of basal cellular organelles, primarily involved in the translation of mrna leading to protein synthesis. however, certain ribosomal proteins moonlight in the development and differentiation of organisms. in this study, the ribosomal protein l44 (rpl44), associated with salt resistance, was screened from the halophilic fungus aspergillus glaucus (agrpl44), and its activity was investigated in saccharomyces cerevisiae and nicotiana tabacum. seque ...201424814782
abiotic stress resistance, a novel moonlighting function of ribosomal protein rpl44 in the halophilic fungus aspergillus glaucus.ribosomal proteins are highly conserved components of basal cellular organelles, primarily involved in the translation of mrna leading to protein synthesis. however, certain ribosomal proteins moonlight in the development and differentiation of organisms. in this study, the ribosomal protein l44 (rpl44), associated with salt resistance, was screened from the halophilic fungus aspergillus glaucus (agrpl44), and its activity was investigated in saccharomyces cerevisiae and nicotiana tabacum. seque ...201424814782
three new polyketides from marine-derived fungus aspergillus glaucus hb1-19.two new benzyl derivatives, aspergentisyl a (1) and aspergentisyl b (2), as well as one new naphthoquinone derivative, aspergiodiquinone (3), together with seven known prenylated benzaldehyde derivatives (4-10) were isolated from the marine-derived fungus aspergillus glaucus hb1-19. the structures of these compounds were characterized based on 1d and 2d nmr spectra analyses and comparison with those reported in the literature. in addition, each isolate was tested for its 1,1-diphenyl-2-picrylhyd ...201323947932
three new polyketides from marine-derived fungus aspergillus glaucus hb1-19.two new benzyl derivatives, aspergentisyl a (1) and aspergentisyl b (2), as well as one new naphthoquinone derivative, aspergiodiquinone (3), together with seven known prenylated benzaldehyde derivatives (4-10) were isolated from the marine-derived fungus aspergillus glaucus hb1-19. the structures of these compounds were characterized based on 1d and 2d nmr spectra analyses and comparison with those reported in the literature. in addition, each isolate was tested for its 1,1-diphenyl-2-picrylhyd ...201323947932
light regulation on growth, development, and secondary metabolism of marine-derived filamentous fungi.effects of different light conditions on development, growth, and secondary metabolism of three marine-derived filamentous fungi were investigated. darkness irritated sexual development of aspergillus glaucus hb1-19, while white, red, and blue lights improved its asexual behavior. the red and blue lights improved asexual stroma formation of xylaria sp. (no. 2508), but the darkness and white light inhibited it. differently, development of halorosellinia sp. (no. 1403) turned out to be insensitive ...201323546832
light regulation on growth, development, and secondary metabolism of marine-derived filamentous fungi.effects of different light conditions on development, growth, and secondary metabolism of three marine-derived filamentous fungi were investigated. darkness irritated sexual development of aspergillus glaucus hb1-19, while white, red, and blue lights improved its asexual behavior. the red and blue lights improved asexual stroma formation of xylaria sp. (no. 2508), but the darkness and white light inhibited it. differently, development of halorosellinia sp. (no. 1403) turned out to be insensitive ...201323546832
improved gene targeting frequency in marine-derived filamentous fungus aspergillus glaucus by disrupting ligd.filamentous fungi from the marine environment have shown great potential as cell factories for the production of pharmacologically active metabolites, but extremely low frequency of homologous recombination brings difficulty to further molecular biology studies. to bypass this problem and develop a highly efficient gene targeting system in marine-derived filamentous fungus aspergillus glaucus, ligd, a homolog of neurospora crassa mus-53 which is considered to play a significant role in nonhomolo ...201222562375
improved gene targeting frequency in marine-derived filamentous fungus aspergillus glaucus by disrupting ligd.filamentous fungi from the marine environment have shown great potential as cell factories for the production of pharmacologically active metabolites, but extremely low frequency of homologous recombination brings difficulty to further molecular biology studies. to bypass this problem and develop a highly efficient gene targeting system in marine-derived filamentous fungus aspergillus glaucus, ligd, a homolog of neurospora crassa mus-53 which is considered to play a significant role in nonhomolo ...201222562375
an integrated control strategy for the fermentation of the marine-derived fungus aspergillus glaucus for the production of anti-cancer polyketide.an integrated control strategy of ph, shear stress, and dissolved oxygen tension (dot) for fermentation scale-up of the marine-derived fungus aspergillus glaucus hb 1–19 for the production of the anti-cancer compound aspergiolide a was studied. keeping initial ph of 6.5 and shifting ph from 6.0 to 7.0 intermittently during the production phase greatly facilitated biosynthesis of aspergiolide a in shake flask cultures. thus, a ph-shift strategy was proposed that shifting ph to 7.0 once it went lo ...201222286337
an integrated control strategy for the fermentation of the marine-derived fungus aspergillus glaucus for the production of anti-cancer polyketide.an integrated control strategy of ph, shear stress, and dissolved oxygen tension (dot) for fermentation scale-up of the marine-derived fungus aspergillus glaucus hb 1–19 for the production of the anti-cancer compound aspergiolide a was studied. keeping initial ph of 6.5 and shifting ph from 6.0 to 7.0 intermittently during the production phase greatly facilitated biosynthesis of aspergiolide a in shake flask cultures. thus, a ph-shift strategy was proposed that shifting ph to 7.0 once it went lo ...201222286337
primary cutaneous aspergillosis from tamilnadu diagnosed by fine needle aspiration cytology.aspergillus are ubiquitous and more than 30 species have been reported to be involved in human infection. most of the cases occur in immunocompromised patients and are disseminated in the blood. primary cutaneous aspergillosis in immunocompetent hosts is rare. we report a unique case of primary cutaneous aspergillosis in an immunocompetent patient diagnosed by fine needle aspiration cytology. the characteristic ascocarp and ascospores of aspergillus species were found in the aspirate and aspergi ...201224371752
primary cutaneous aspergillosis from tamilnadu diagnosed by fine needle aspiration cytology.aspergillus are ubiquitous and more than 30 species have been reported to be involved in human infection. most of the cases occur in immunocompromised patients and are disseminated in the blood. primary cutaneous aspergillosis in immunocompetent hosts is rare. we report a unique case of primary cutaneous aspergillosis in an immunocompetent patient diagnosed by fine needle aspiration cytology. the characteristic ascocarp and ascospores of aspergillus species were found in the aspirate and aspergi ...201224371752
mycobiota and aflatoxin b1 in feed for farmed sea bass (dicentrarchus labrax).thesafety characteristics of feed used in fish and crustacean aquaculture systems are an essential tool to assure the productivity of those animal exploitations. safety of feed may be affected by different hazards, including biological and chemical groups. the aim of this preliminary study was to evaluate fungi contamination and the presence of aflatoxins in 87 samples of feed for sea bass, collected in portugal. molds were found in 35 samples (40.2%) in levels ranging from 1 to 3.3 log10 cfu∙g( ...201122069703
mycobiota and aflatoxin b1 in feed for farmed sea bass (dicentrarchus labrax).thesafety characteristics of feed used in fish and crustacean aquaculture systems are an essential tool to assure the productivity of those animal exploitations. safety of feed may be affected by different hazards, including biological and chemical groups. the aim of this preliminary study was to evaluate fungi contamination and the presence of aflatoxins in 87 samples of feed for sea bass, collected in portugal. molds were found in 35 samples (40.2%) in levels ranging from 1 to 3.3 log10 cfu∙g( ...201122069703
cellulase hydrolysis of rice straw and inactivation of endoglucanase in urea solution.in order to optimize the cellulase (from aspergillus glaucus) hydrolysis of pretreated rice straw, the effects of varying enzyme concentration, temperature, and ph were studied. the best experimental conditions found to degrade the pretreated rice straws were 24 h of incubation at 55 °c and ph 5.0, with an enzyme concentration of 48 mg/l. urea is one of the important nitrogen sources used in fungi culture, but it is also a denaturant. the model of denaturation of endoglucanase (eg) in urea solut ...201121919515
cellulase hydrolysis of rice straw and inactivation of endoglucanase in urea solution.in order to optimize the cellulase (from aspergillus glaucus) hydrolysis of pretreated rice straw, the effects of varying enzyme concentration, temperature, and ph were studied. the best experimental conditions found to degrade the pretreated rice straws were 24 h of incubation at 55 °c and ph 5.0, with an enzyme concentration of 48 mg/l. urea is one of the important nitrogen sources used in fungi culture, but it is also a denaturant. the model of denaturation of endoglucanase (eg) in urea solut ...201121919515
Liriodenine, early antimicrobial defence in Annona diversifolia.Annonaceae aporphine alkaloids, of which liriodenine is the most abundant, have not been extensively studied from a biological standpoint. The goal of this study was to investigate the role of liriodenine in antimicrobial defense during early developmental stages in Annona diversifolia. The fungi Rhizopus stolonifer and Aspergillus glaucus, which are responsible for seed deterioration, were isolated during imbibition, and their antifungal activity was determined by diffusion, macrodilution, and ...201121950162
Liriodenine, early antimicrobial defence in Annona diversifolia.Annonaceae aporphine alkaloids, of which liriodenine is the most abundant, have not been extensively studied from a biological standpoint. The goal of this study was to investigate the role of liriodenine in antimicrobial defense during early developmental stages in Annona diversifolia. The fungi Rhizopus stolonifer and Aspergillus glaucus, which are responsible for seed deterioration, were isolated during imbibition, and their antifungal activity was determined by diffusion, macrodilution, and ...201121950162
identification of fungi associated with rotylenchulus reniformis.the objective of this work was to isolate and identify fungi associated with r. reniformis in cotton roots. soil samples were collected in cotton fields naturally infested with r. reniformis and from cotton stock plants cultured in the greenhouse. nematodes extracted from the soil were observed under the stereoscope, and discolored eggs and vermiform stages colonized with mycelia were cultured on 1.5% water agar supplemented with antibiotics, and incubated at 27°c. identification of the nematoph ...201022736864
identification of fungi associated with rotylenchulus reniformis.the objective of this work was to isolate and identify fungi associated with r. reniformis in cotton roots. soil samples were collected in cotton fields naturally infested with r. reniformis and from cotton stock plants cultured in the greenhouse. nematodes extracted from the soil were observed under the stereoscope, and discolored eggs and vermiform stages colonized with mycelia were cultured on 1.5% water agar supplemented with antibiotics, and incubated at 27°c. identification of the nematoph ...201022736864
significant stimulation of o-phthalic acid in biosynthesis of aspergiolide a by a marine fungus aspergillus glaucus.the effect of o-phthalic acid (o-pa) on the production of the anti-tumor polyketide compound aspergiolide a by the marine fungus aspergillus glaucus was investigated. o-pa at 12mm increased the aspergiolide a production by 77%. a combination of 12mm acetate with 12mm o-pa increased the production by 126%, i.e., from 27.1mg/l to 60.9mg/l, which was 27% and 75% higher than adding either 12mm o-pa or 12mm acetate, respectively. it was also found that a. glaucus could use o-pa as the sole carbon sou ...201020100655
significant stimulation of o-phthalic acid in biosynthesis of aspergiolide a by a marine fungus aspergillus glaucus.the effect of o-phthalic acid (o-pa) on the production of the anti-tumor polyketide compound aspergiolide a by the marine fungus aspergillus glaucus was investigated. o-pa at 12mm increased the aspergiolide a production by 77%. a combination of 12mm acetate with 12mm o-pa increased the production by 126%, i.e., from 27.1mg/l to 60.9mg/l, which was 27% and 75% higher than adding either 12mm o-pa or 12mm acetate, respectively. it was also found that a. glaucus could use o-pa as the sole carbon sou ...201020100655
efficient strategy for enhancing aspergiolide a production by citrate feedings and its effects on sexual development and growth of marine-derived fungus aspergillus glaucus.aspergiolide a production enhancement by citrate and its effects on growth and sexual development of marine-derived fungus aspergillus glaucus hb1-19 were investigated. in agar plate culture, 15 mm citric acid decreased colony radial growth and aspergiolide a production by 31.5% and 23.0%, respectively. it also improved sexual cleistothecium formation by 360% but depressed asexual conidiospore generation by 84.8%. in submerged culture, adding 40 mm citric acid finally promoted aspergiolide a pro ...201020359887
efficient strategy for enhancing aspergiolide a production by citrate feedings and its effects on sexual development and growth of marine-derived fungus aspergillus glaucus.aspergiolide a production enhancement by citrate and its effects on growth and sexual development of marine-derived fungus aspergillus glaucus hb1-19 were investigated. in agar plate culture, 15 mm citric acid decreased colony radial growth and aspergiolide a production by 31.5% and 23.0%, respectively. it also improved sexual cleistothecium formation by 360% but depressed asexual conidiospore generation by 84.8%. in submerged culture, adding 40 mm citric acid finally promoted aspergiolide a pro ...201020359887
purification and properties of endoglucanase from a sugar cane bagasse hydrolyzing strain, aspergillus glaucus xc9.an endoglucanase (eg) from aspergillus glaucus xc9 grown on 0.3% sugar cane bagasse as a carbon source was purified from the culture filtrate using ammonium sulfate, an anion exchange deae sepharose fast flow column, and a sephadex g-100 column, with a purification fold of 21.5 and a recovery of 22.3%. the ideal time for eg production is on the fourth day at 30 degrees c using bagasse as a substrate. results obtained indicate that the enzyme was a monomer protein, and the molecular weight was de ...201020415423
purification and properties of endoglucanase from a sugar cane bagasse hydrolyzing strain, aspergillus glaucus xc9.an endoglucanase (eg) from aspergillus glaucus xc9 grown on 0.3% sugar cane bagasse as a carbon source was purified from the culture filtrate using ammonium sulfate, an anion exchange deae sepharose fast flow column, and a sephadex g-100 column, with a purification fold of 21.5 and a recovery of 22.3%. the ideal time for eg production is on the fourth day at 30 degrees c using bagasse as a substrate. results obtained indicate that the enzyme was a monomer protein, and the molecular weight was de ...201020415423
enhancing aspergiolide a production from a shear-sensitive and easy-foaming marine-derived filamentous fungus aspergillus glaucus by oxygen carrier addition and impeller combination in a bioreactor.production enhancement of a novel antitumor compound aspergiolide a from shear-sensitive and easy-foaming marine-derived fungus aspergillus glaucus hb1-19 in a 5-l stirred bioreactor was investigated. two types of impellers, i.e., six-flat-blade disc turbine impeller (dt) and three-sector-blade pitched blade turbine impeller (pb) were used in this work. in cultures with fermentation medium, the combination of upper pb and lower dt led to the maximum dry biomass (13.8 g/l) and aspergiolide a prod ...201021074418
enhancing aspergiolide a production from a shear-sensitive and easy-foaming marine-derived filamentous fungus aspergillus glaucus by oxygen carrier addition and impeller combination in a bioreactor.production enhancement of a novel antitumor compound aspergiolide a from shear-sensitive and easy-foaming marine-derived fungus aspergillus glaucus hb1-19 in a 5-l stirred bioreactor was investigated. two types of impellers, i.e., six-flat-blade disc turbine impeller (dt) and three-sector-blade pitched blade turbine impeller (pb) were used in this work. in cultures with fermentation medium, the combination of upper pb and lower dt led to the maximum dry biomass (13.8 g/l) and aspergiolide a prod ...201021074418
aspergiolides c and d: spirocyclic aromatic polyketides with potent protein kinase c-met inhibitory effects.variation of the cultivation conditions for aspergillus glaucus led to the discovery of two novel spirocyclic aromatic polyketides, aspergiolides c (3) and d (4). their constitutions were elucidated by a combination of spectroscopic methods and isotope-labeling experiments. aspergiolides c (3) and d (4) occur as racemic mixtures, the resolution of which was succeeded by hplc on a chiral phase. the absolute configurations of their enantiomers were assigned online, from the peaks in the chromatogr ...201021132693
aspergiolides c and d: spirocyclic aromatic polyketides with potent protein kinase c-met inhibitory effects.variation of the cultivation conditions for aspergillus glaucus led to the discovery of two novel spirocyclic aromatic polyketides, aspergiolides c (3) and d (4). their constitutions were elucidated by a combination of spectroscopic methods and isotope-labeling experiments. aspergiolides c (3) and d (4) occur as racemic mixtures, the resolution of which was succeeded by hplc on a chiral phase. the absolute configurations of their enantiomers were assigned online, from the peaks in the chromatogr ...201021243700
aspergiolides c and d: spirocyclic aromatic polyketides with potent protein kinase c-met inhibitory effects.variation of the cultivation conditions for aspergillus glaucus led to the discovery of two novel spirocyclic aromatic polyketides, aspergiolides c (3) and d (4). their constitutions were elucidated by a combination of spectroscopic methods and isotope-labeling experiments. aspergiolides c (3) and d (4) occur as racemic mixtures, the resolution of which was succeeded by hplc on a chiral phase. the absolute configurations of their enantiomers were assigned online, from the peaks in the chromatogr ...201021243700
identified biosynthetic pathway of aspergiolide a and a novel strategy to increase its production in a marine-derived fungus aspergillus glaucus by feeding of biosynthetic precursors and inhibitors simultaneously.aspergiolide a is a novel anti-tumor anthraquinone derivant produced by marine-derived fungus aspergillus glaucus. to identify its biosynthetic pathway and further improve the production, the effects of biosynthetic pathway specific inhibitors and precursors were investigated. cerulenin and iodoacetamide, the specific inhibitors of polyketide pathway, could completely inhibit the aspergiolide a accumulation. putative precursors of polyketide pathway could increase aspergiolide a production great ...200919386490
identified biosynthetic pathway of aspergiolide a and a novel strategy to increase its production in a marine-derived fungus aspergillus glaucus by feeding of biosynthetic precursors and inhibitors simultaneously.aspergiolide a is a novel anti-tumor anthraquinone derivant produced by marine-derived fungus aspergillus glaucus. to identify its biosynthetic pathway and further improve the production, the effects of biosynthetic pathway specific inhibitors and precursors were investigated. cerulenin and iodoacetamide, the specific inhibitors of polyketide pathway, could completely inhibit the aspergiolide a accumulation. putative precursors of polyketide pathway could increase aspergiolide a production great ...200919386490
statistical optimization of medium composition for aspergiolide a production by marine-derived fungus aspergillus glaucus.statistical methodologies were employed to optimize submerged culture medium for the production of a novel antineoplastic compound aspergiolide a by a marine-derived fungus aspergillus glaucus hb1-19 for the first time. orthogonal design was preformed to determine the initial composition. then plackett-burman design was applied to evaluate the influence of related nutrients, and yeast extract paste, soybean powder and sodium glutamate were confirmed as critical factors in the medium. response su ...200919104862
[a case of hypersensitivity pneumonitis resulting from inhalation of aspergillus niger in a greenhouse worker who raised roses].a 57-year-old woman was referred because of exertional dyspnea, fever, and cough in june 2006. she had been employed to culture roses in greenhouses since 1991 and had developed a cough during the summer from 2003. chest ct scan revealed diffuse centrilobular micronodules. transbronchial lung biopsy specimens demonstrated alveolitis with lymphocytes and non-necrotizing epithelioid cell granulomas. after admission, both the patient's symptoms and laboratory data improved without medication. howev ...200919348267
[a case of hypersensitivity pneumonitis resulting from inhalation of aspergillus niger in a greenhouse worker who raised roses].a 57-year-old woman was referred because of exertional dyspnea, fever, and cough in june 2006. she had been employed to culture roses in greenhouses since 1991 and had developed a cough during the summer from 2003. chest ct scan revealed diffuse centrilobular micronodules. transbronchial lung biopsy specimens demonstrated alveolitis with lymphocytes and non-necrotizing epithelioid cell granulomas. after admission, both the patient's symptoms and laboratory data improved without medication. howev ...200919348267
mycobiota and concentration of ochratoxin a in concentrated poultry feed from venezuela.the main objective of this study was to evaluate the frequency distribution of mycobiota and the concentration of ochratoxin a (ota) in 50 samples from one company of commercial brand poultry feed produced in venezuela. the concentration of ota in the samples analyzed was determined using the competitive elisa method. the most frequently isolated genera of moulds were aspergillus (36%) and penicillium (20%). of these genera, the most frequently isolated species were aspergillus flavus, aspergill ...200919580016
mycobiota and concentration of ochratoxin a in concentrated poultry feed from venezuela.the main objective of this study was to evaluate the frequency distribution of mycobiota and the concentration of ochratoxin a (ota) in 50 samples from one company of commercial brand poultry feed produced in venezuela. the concentration of ota in the samples analyzed was determined using the competitive elisa method. the most frequently isolated genera of moulds were aspergillus (36%) and penicillium (20%). of these genera, the most frequently isolated species were aspergillus flavus, aspergill ...200919580016
statistical optimization of medium composition for aspergiolide a production by marine-derived fungus aspergillus glaucus.statistical methodologies were employed to optimize submerged culture medium for the production of a novel antineoplastic compound aspergiolide a by a marine-derived fungus aspergillus glaucus hb1-19 for the first time. orthogonal design was preformed to determine the initial composition. then plackett-burman design was applied to evaluate the influence of related nutrients, and yeast extract paste, soybean powder and sodium glutamate were confirmed as critical factors in the medium. response su ...200919104862
cytotoxic polyketides from a marine-derived fungus aspergillus glaucus.eight new aromatic polyketides (2, 4-6, 8, 14, 16, and 17) together with eight known analogues (3, 7, 9-13, and 15) were isolated from the marine-derived fungus aspergillus glaucus. the structures and stereochemistry of the new compounds were elucidated by spectroscopic and chemical methods, and their cytotoxicities were evaluated against the hl-60 and a-549 cell lines.200818986198
cytotoxic polyketides from a marine-derived fungus aspergillus glaucus.eight new aromatic polyketides (2, 4-6, 8, 14, 16, and 17) together with eight known analogues (3, 7, 9-13, and 15) were isolated from the marine-derived fungus aspergillus glaucus. the structures and stereochemistry of the new compounds were elucidated by spectroscopic and chemical methods, and their cytotoxicities were evaluated against the hl-60 and a-549 cell lines.200818986198
composition and antifungal activity of the essential oil of the brazilian chenopodium ambrosioides l.the antifungal activity of essential oil (eo) from the brazilian epazote (chenopodium ambrosioides l.) was evaluated by the poison food assay at concentrations of 0.3%, 0.1%, and 0.05% with eight postharvest deteriorating fungi (aspergillus flavus, aspergillus glaucus, aspergillus niger, aspergillus ochraceous, colletotrichum gloesporioides, colletotrichum musae, fusarium oxysporum, and fusarium semitectum). eo components were tentatively identified by kováts retention indices (ris) using gas ch ...200818679750
composition and antifungal activity of the essential oil of the brazilian chenopodium ambrosioides l.the antifungal activity of essential oil (eo) from the brazilian epazote (chenopodium ambrosioides l.) was evaluated by the poison food assay at concentrations of 0.3%, 0.1%, and 0.05% with eight postharvest deteriorating fungi (aspergillus flavus, aspergillus glaucus, aspergillus niger, aspergillus ochraceous, colletotrichum gloesporioides, colletotrichum musae, fusarium oxysporum, and fusarium semitectum). eo components were tentatively identified by kováts retention indices (ris) using gas ch ...200818679750
mycoflora of two types of portuguese dry-smoked sausages and inhibitory effect of sodium benzoate, potassium sorbate, and methyl p-hydroxybenzoate on mold growth rate.the mycoflora of chouriqo types alentejano and ribatejano, two varieties of portuguese dry-smoked sausages, have been investigated after a producer-defined shelf life period (120 days at 20 +/- 5 degrees c) in modified atmosphere packaging (55% n2 and 45% co2). on the basis of morphological and physiological characteristics, the isolates were identified as penicillium, aspergillus, fusarium, rhizopus, monilia, absidia, and cephalosporium. the species identified were as follows: penicillium terre ...200717612078
mycoflora of two types of portuguese dry-smoked sausages and inhibitory effect of sodium benzoate, potassium sorbate, and methyl p-hydroxybenzoate on mold growth rate.the mycoflora of chouriqo types alentejano and ribatejano, two varieties of portuguese dry-smoked sausages, have been investigated after a producer-defined shelf life period (120 days at 20 +/- 5 degrees c) in modified atmosphere packaging (55% n2 and 45% co2). on the basis of morphological and physiological characteristics, the isolates were identified as penicillium, aspergillus, fusarium, rhizopus, monilia, absidia, and cephalosporium. the species identified were as follows: penicillium terre ...200717612078
fatal brain infection caused by aspergillus glaucus in an immunocompetent patient identified by sequencing of the ribosomal 18s-28s internal transcribed spacer.cerebral aspergillosis has rarely been reported in immunocompetent patients. we herein describe a unique case of cerebral aspergillosis in a healthy adult that led to his death despite aggressive antifungal therapy. sequencing of ribosomal 18s-28s internal transcribed spacer identified the organism as eurotium herbariorum, the teleomorph of aspergillus glaucus.200717665232
fatal brain infection caused by aspergillus glaucus in an immunocompetent patient identified by sequencing of the ribosomal 18s-28s internal transcribed spacer.cerebral aspergillosis has rarely been reported in immunocompetent patients. we herein describe a unique case of cerebral aspergillosis in a healthy adult that led to his death despite aggressive antifungal therapy. sequencing of ribosomal 18s-28s internal transcribed spacer identified the organism as eurotium herbariorum, the teleomorph of aspergillus glaucus.200717665232
susceptibility of environmental versus clinical strains of pathogenic aspergillus.the objective of this study was to compare the antifungal susceptibility profiles of 307 environmental strains and 139 clinical isolates of aspergillus belonging to six different species. clinical and environmental strains with minimal inhibitory concentrations (mics) or minimal effective concentrations >or=4microg/ml to amphotericin b (amb), itraconazole (itc), voriconazole and caspofungin were seldom detected. however, the susceptibility profile of environmental aspergillus non-fumigatus strai ...200717189101
susceptibility of environmental versus clinical strains of pathogenic aspergillus.the objective of this study was to compare the antifungal susceptibility profiles of 307 environmental strains and 139 clinical isolates of aspergillus belonging to six different species. clinical and environmental strains with minimal inhibitory concentrations (mics) or minimal effective concentrations >or=4microg/ml to amphotericin b (amb), itraconazole (itc), voriconazole and caspofungin were seldom detected. however, the susceptibility profile of environmental aspergillus non-fumigatus strai ...200717189101
antifungal efficacy of hydrogen peroxide in dental unit waterline disinfection.the concentration and composition of fungal flora in dental unit waterlines (duwl) were evaluated. for this purpose, water samples from unit reservoirs and high-speed handpieces, and biofilm samples from the waterline walls from units were collected. subsequently, analogous samples from duwl were taken before and after disinfection using agent containing hydrogen peroxide. in the examined samples, the yeast-like fungi candida albicans and candida curvata were found. the following species of moul ...200617196007
antifungal efficacy of hydrogen peroxide in dental unit waterline disinfection.the concentration and composition of fungal flora in dental unit waterlines (duwl) were evaluated. for this purpose, water samples from unit reservoirs and high-speed handpieces, and biofilm samples from the waterline walls from units were collected. subsequently, analogous samples from duwl were taken before and after disinfection using agent containing hydrogen peroxide. in the examined samples, the yeast-like fungi candida albicans and candida curvata were found. the following species of moul ...200617196007
secretion of keratinolytic enzymes and keratinolysis by scopulariopsis brevicaulis and trichophyton mentagrophytes: regression analysis.a survey on keratinophilic fungi from poultry-farm soils at namakkal and from feather dumping soils at chennai, india, revealed the existence of 34 species of fungi. most of the fungi exhibited variable efficiency in producing extracellular keratinase when grown in plates with chicken feathers as the sole carbon and nitrogen source. the fungi aspergillus flavus, aspergillus niger, aspergillus versicolor, chrysosporium state of arthroderma tuberculatum, paecilomyces carneus, scopulariopsis brevic ...200617215897
secretion of keratinolytic enzymes and keratinolysis by scopulariopsis brevicaulis and trichophyton mentagrophytes: regression analysis.a survey on keratinophilic fungi from poultry-farm soils at namakkal and from feather dumping soils at chennai, india, revealed the existence of 34 species of fungi. most of the fungi exhibited variable efficiency in producing extracellular keratinase when grown in plates with chicken feathers as the sole carbon and nitrogen source. the fungi aspergillus flavus, aspergillus niger, aspergillus versicolor, chrysosporium state of arthroderma tuberculatum, paecilomyces carneus, scopulariopsis brevic ...200617215897
exposure to airborne fungi during conservative dental treatment.the aim of the study was a mycological assessment of bioaerosol forming during conservative dental treatment, taking into account concentration and type of fungal microflora, and evaluation of the influence of duwl disinfecting protocol on the fungal contamination of the bioaerosol. the research was conducted on 25 operative sites located in public dental clinics. the air contained in the space between a patient and a dentist during conservative dental treatment with the use of a high-speed hand ...200616841889
exposure to airborne fungi during conservative dental treatment.the aim of the study was a mycological assessment of bioaerosol forming during conservative dental treatment, taking into account concentration and type of fungal microflora, and evaluation of the influence of duwl disinfecting protocol on the fungal contamination of the bioaerosol. the research was conducted on 25 operative sites located in public dental clinics. the air contained in the space between a patient and a dentist during conservative dental treatment with the use of a high-speed hand ...200616841889
aspergillus surveillance project at a large tertiary-care hospital.a one-year surveillance project was conducted at a large tertiary hospital, which had extensive indoor renovation and extensive demolition/building at several nearby sites. this study collected viable fungi samples in the hospital every six days and analysed 74 duct dust samples for aspergillus fumigatus mycelial asp f1 protein. mean total fungi were 257.8 cfu/m3 outdoors, 53.2 cfu/m3 in all indoor samples and 83.5 cfu/m3 in the bone marrow transplant patient rooms. mean total aspergillus was 6. ...200515694975
aspergillus surveillance project at a large tertiary-care hospital.a one-year surveillance project was conducted at a large tertiary hospital, which had extensive indoor renovation and extensive demolition/building at several nearby sites. this study collected viable fungi samples in the hospital every six days and analysed 74 duct dust samples for aspergillus fumigatus mycelial asp f1 protein. mean total fungi were 257.8 cfu/m3 outdoors, 53.2 cfu/m3 in all indoor samples and 83.5 cfu/m3 in the bone marrow transplant patient rooms. mean total aspergillus was 6. ...200515694975
evaluation of mycological contamination of dental unit waterlines.the quality of dental unit water is of great importance since patients and dental staff are regularly exposed to water from aerosols generated during work. the main purpose of this investigation was mycological evaluation of dental unit waterlines (duwl). the author determined the number and species of fungi present in the water from a unit reservoir which is the source of water for a dental unit, in the water flowing from a high-speed handpiece of a unit, and in the swab sample collected from t ...200516028882
evaluation of mycological contamination of dental unit waterlines.the quality of dental unit water is of great importance since patients and dental staff are regularly exposed to water from aerosols generated during work. the main purpose of this investigation was mycological evaluation of dental unit waterlines (duwl). the author determined the number and species of fungi present in the water from a unit reservoir which is the source of water for a dental unit, in the water flowing from a high-speed handpiece of a unit, and in the swab sample collected from t ...200516028882
detection and identification of fungi from fungus balls of the maxillary sinus by molecular techniques.the aim of this study was to find a reliable method for the detection and identification of fungi in fungus balls of the maxillary sinus and to evaluate the spectrum of fungi in these samples. one hundred twelve samples were obtained from patients with histologically proven fungal infections; 81 samples were paraffin-embedded tissue sections of the maxillary sinus. in 31 cases, sinus contents without paraffin embedding were sent for investigation. pcr amplification with universal fungal primers ...200312574250
detection and identification of fungi from fungus balls of the maxillary sinus by molecular techniques.the aim of this study was to find a reliable method for the detection and identification of fungi in fungus balls of the maxillary sinus and to evaluate the spectrum of fungi in these samples. one hundred twelve samples were obtained from patients with histologically proven fungal infections; 81 samples were paraffin-embedded tissue sections of the maxillary sinus. in 31 cases, sinus contents without paraffin embedding were sent for investigation. pcr amplification with universal fungal primers ...200312574250
comparison of the sensititre yeastone colorimetric antifungal panel and etest with the nccls m38-a method to determine the activity of amphotericin b and itraconazole against clinical isolates of aspergillus spp.the evaluation of the sensititre yeastone and etest methods for determining susceptibility to amphotericin b and itraconazole by comparing the mics obtained by these methods at different times of reading with those of the m38-a broth microdilution method.200312917236
comparison of the sensititre yeastone colorimetric antifungal panel and etest with the nccls m38-a method to determine the activity of amphotericin b and itraconazole against clinical isolates of aspergillus spp.the evaluation of the sensititre yeastone and etest methods for determining susceptibility to amphotericin b and itraconazole by comparing the mics obtained by these methods at different times of reading with those of the m38-a broth microdilution method.200312917236
polythene and plastic-degrading microbes in an indian mangrove soil.biodegradation of polythene bags and plastic cups was analyzed after 2, 4, 6, and 9 months of incubation in the mangrove soil. the biodegradation of polythene bags was significantly higher (up to 4.21% in 9 months) than that of plastic cups (up to 0.25% in 9 months). microbial counts in the degrading materials were recorded up to 79.67 x 10(4) per gram for total heterotrophic bacteria, and up to 55.33 x 10(2) per gram for fungi. the microbial species found associated with the degrading materials ...200315162769
polythene and plastic-degrading microbes in an indian mangrove soil.biodegradation of polythene bags and plastic cups was analyzed after 2, 4, 6, and 9 months of incubation in the mangrove soil. the biodegradation of polythene bags was significantly higher (up to 4.21% in 9 months) than that of plastic cups (up to 0.25% in 9 months). microbial counts in the degrading materials were recorded up to 79.67 x 10(4) per gram for total heterotrophic bacteria, and up to 55.33 x 10(2) per gram for fungi. the microbial species found associated with the degrading materials ...200315162769
exposure to microbes, endotoxins and total dust in cigarette and cigar manufacturing: an evaluation of health hazards.the concentrations of airborne microbes, endotoxins and total dust were measured in one cigar and two cigarette factories in order to evaluate the risk of respiratory symptoms. the role of humidifiers as a source of microbes was investigated. air samples for the analyses were collected near workers' breathing zones during different phases of production. gram-negative bacteria, mesophilic fungi, thermotolerant fungi and thermophilic actinomycetes, but not aspergillus glaucus fungi, were found in ...200010963711
exposure to microbes, endotoxins and total dust in cigarette and cigar manufacturing: an evaluation of health hazards.the concentrations of airborne microbes, endotoxins and total dust were measured in one cigar and two cigarette factories in order to evaluate the risk of respiratory symptoms. the role of humidifiers as a source of microbes was investigated. air samples for the analyses were collected near workers' breathing zones during different phases of production. gram-negative bacteria, mesophilic fungi, thermotolerant fungi and thermophilic actinomycetes, but not aspergillus glaucus fungi, were found in ...200010963711
molds on house walls and the effect of their chloroform-extractable metabolites on the respiratory cilia movement of one-day-old chicks in vitro.the ciliostatic activity of the chloroform-extractable endo- and exometabolites of 5 strains of filamentous fungi--alternaria sp., aspergillus glaucus group, aspergillus versicolor, cladosporium sphoerospermum, penicillium sp. and ulocladium sp.--isolated from molded walls of a dwelling--on tracheal cilia from 1-d-old chicks in vitro was evaluated. endometabolites of alternaria sp. and a. versicolor and exometabolites of ulocladium sp. were the most active, these extracts stopped the ciliary mov ...199810069012
molds on house walls and the effect of their chloroform-extractable metabolites on the respiratory cilia movement of one-day-old chicks in vitro.the ciliostatic activity of the chloroform-extractable endo- and exometabolites of 5 strains of filamentous fungi--alternaria sp., aspergillus glaucus group, aspergillus versicolor, cladosporium sphoerospermum, penicillium sp. and ulocladium sp.--isolated from molded walls of a dwelling--on tracheal cilia from 1-d-old chicks in vitro was evaluated. endometabolites of alternaria sp. and a. versicolor and exometabolites of ulocladium sp. were the most active, these extracts stopped the ciliary mov ...199810069012
contamination of microscope slides with aspergillus glaucus group a: a laboratory problem in the diagnosis of suspected mycotic infections.the microscopic examination of lesions of patients with suspected mycotic infections using slides purchased from foreign countries often showed hyphae. the slides and their wrappings were cultured successfully on sabouraud dextrose-agar medium. a heavy growth of suspected aspergillus colonies was obtained. these colonies were investigated further by culturing them on both czapek's solution agar and malt extract agar. after macroscopic and microscopic examination the fungus was identified as aspe ...19979301558
contamination of microscope slides with aspergillus glaucus group a: a laboratory problem in the diagnosis of suspected mycotic infections.the microscopic examination of lesions of patients with suspected mycotic infections using slides purchased from foreign countries often showed hyphae. the slides and their wrappings were cultured successfully on sabouraud dextrose-agar medium. a heavy growth of suspected aspergillus colonies was obtained. these colonies were investigated further by culturing them on both czapek's solution agar and malt extract agar. after macroscopic and microscopic examination the fungus was identified as aspe ...19979301558
detection of aspergillus ribosomal rna using biotinylated oligonucleotide probes.aspergillosis continues to be a devastating disease entity that results in significant mortality in immunosuppressed patients. rapid diagnosis is often required to initiate appropriate therapy. although the histopathologist may be able to visualize fungal organisms in tissue specimens, the histology of aspergillus species may overlap with a variety of fungi, so diagnosis often relies on fungal cultures that can take weeks to complete. recently, an in situ hybridization assay targeting aspergillu ...19979458383
detection of aspergillus ribosomal rna using biotinylated oligonucleotide probes.aspergillosis continues to be a devastating disease entity that results in significant mortality in immunosuppressed patients. rapid diagnosis is often required to initiate appropriate therapy. although the histopathologist may be able to visualize fungal organisms in tissue specimens, the histology of aspergillus species may overlap with a variety of fungi, so diagnosis often relies on fungal cultures that can take weeks to complete. recently, an in situ hybridization assay targeting aspergillu ...19979458383
molecular probes for diagnosis of fungal infections.we have developed 21 specific nucleic acid probes which target the large subunit rrna genes from aspergillus flavus, aspergillus fumigatus, aspergillus glaucus, aspergillus niger, aspergillus terreus, blastomyces dermatitidis, candida albicans, candida (torulopsis) glabrata, candida guilliermondii, candida kefyr, candida krusei, candida lusitaniae, candida parapsilosis, candida tropicalis, coccidioides immitis, cryptococcus neoformans var. gattii, cryptococcus neoformans var. neoformans, filobas ...19958576345
molecular probes for diagnosis of fungal infections.we have developed 21 specific nucleic acid probes which target the large subunit rrna genes from aspergillus flavus, aspergillus fumigatus, aspergillus glaucus, aspergillus niger, aspergillus terreus, blastomyces dermatitidis, candida albicans, candida (torulopsis) glabrata, candida guilliermondii, candida kefyr, candida krusei, candida lusitaniae, candida parapsilosis, candida tropicalis, coccidioides immitis, cryptococcus neoformans var. gattii, cryptococcus neoformans var. neoformans, filobas ...19958576345
one hundred years ago: the history of cryptococcosis in greifswald. medical mycology in the nineteenth century.medico-mycological investigations began at greifswald in germany as early as 1842 when wilhelm baum (1799-1883) was appointed to the chair of surgery of the university. this is shown by some theses of the time, as well as by the discovery of the contagious nature of pityriasis versicolor by carl ferdinand eichstedt (1816-92), who identified a fungus as the cause (1846), later named microsporon furfur (c. robin 1853). in 1868 the physician karl friedrich mosler (1831-1911) published clinical-myco ...19947739651
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