| hair and scalp mycobiota in school children in nablus area. | hair and scalp mycobiota of 1389 clinically normal children aged 6-12 years attending 12 schools in the nablus district, palestinian authority, was assessed on three occasions over 8-month period (october 1998-may 1999) using the hair brush technique. one hundred and one fungal species belonging to 33 genera were recovered: 6 dermatophytes, 16 dermatophyte-like keratinophilic fungi, and 79 other keratinophilic fungal species. species varied considerably in their frequency of occurrence and abund ... | 2001 | 11469760 |
| isolation and characterization of mn(iii) tartrate from phanerochaete chrysosporium culture broth. | high initial mn(ii) concentration results in accumulation of a mn(iii) tartrate complex in the growth medium of phanerochaete chrysosporium. since mn(iii) is the major oxidant in ligninolysis by manganese peroxidase, the role of accumulated complex should not be neglected when degradation experiments by a crude culture filtrate are performed. to study the mn(iii) complex oxidative potential it was isolated by absorption to polyamide followed by desorption with an alkaline methanol solution. high ... | 2001 | 11470372 |
| chrysosporium tropicum efficacy against anopheles stephensi larvae in the laboratory. | the keratinophilic soil fungus chrysosporium tropicum was evaluated as a biological control agent against anopheles stephensi larvae in the laboratory. culture filtrates of c. tropicum were found to be toxic to an. stephensi larvae at various concentrations. the ranges of the 50% lethal concentration (lc50) values of fungal filtrates were 16.60-17.78, 12.02-12.88, and 34.67-35.48 microl/ml against 1st-, 2nd-, and 3rd-stage larvae, respectively. the ranges of lc99 values were 38.90-63.10, 12.02-2 ... | 2001 | 11480820 |
| tmc-69, a new antitumor antibiotic with cdc25a inhibitory activity, produced by chrysosporium sp. tc1068. taxonomy, fermentation and biological activities. | a new antibiotic designated tmc-69 has been isolated from the fermentation broth of a fungal strain chrysosporium sp. tc 1068. tmc-69 exhibited moderate in vitro cytotoxic activity. tmc-69-6h, a derivative of tmc-69 prepared by hydrogenation, possessed more potent in vitro cytotoxicity than tmc-69, and exhibited in vivo antitumor activity against murine p388 leukemia and b16 melanoma. tmc-69-6h was found to specifically inhibit cdc25a and b phosphatases. | 2001 | 11480885 |
| oxidation of triphenylarsine to triphenylarsineoxide by trichoderma harzianum and other fungi. | arsenic resistant strains of bacteria and fungi were isolated from soil contaminated by chemical warfare agents. until now, no metabolic products of microbial attack against the phenyl residues of the model substrate triphenylarsine (tp) were found if it was incubated together with these strains in liquid culture assays. however, one of the isolated fungi, trichoderma harzianum as 11, was found to oxidize tp to triphenylarsineoxide (tpo). the yeast trichosporon mucoides sbug 801 and the white-ro ... | 2001 | 11482658 |
| biodegradation of bioaccessible textile azo dyes by phanerochaete chrysosporium. | azo dyes are important chemical pollutants of industrial origin. textile azo dyes with bioaccessible groups for lignin degrading fungi, such as 2-methoxyphenol (guaiacol) and 2,6-dimethoxyphenol (syringol), were synthesised using different aminobenzoic and aminosulphonic acids as diazo components. the inocula of the best biodegradation assays were obtained from a pre-growth medium (pam), containing one of the synthesised dyes. the results of the dye biodegradation assays were evaluated every 7 d ... | 2001 | 11500201 |
| a packed-bed fungal bioreactor for the continuous decolourisation of azo-dyes (orange ii). | the degradation of an azo dye, orange ii, by immobilised phanerochaete chrysosporium in a continuous packed bed bioreactor for periods longer than 30 days has been carried out. nearly complete decolourisation (>95%) was achieved when working at a high dye load rate of 0.2 g x l(-1) x d(-1), a temperature of 37 degrees c, a hydraulic retention time (hrt) of 24 h and applying oxygen gas in a pulsed flow. these conditions allowed manganese peroxidase (mnp) production and the subsequently orange ii ... | 2001 | 11500202 |
| decolorization of textile indigo dye by ligninolytic fungi. | the indigo dye is extensively used by textile industries and is considered a recalcitrant substance, which causes environmental concern. chemical products used on textile processing, which affect the environment through effluents, can be voluminous, colored and varied. vat textile dyes, like indigo, are often used and dye mainly cellulosic fibers of cotton. decolorization of this dye in liquid medium was tested with ligninolytic basidiomycete fungi from brazil. decolorization started in a few ho ... | 2001 | 11500207 |
| application of microassays for investigation of cellulase abrasive activity and backstaining. | model microassays were used for testing the denim-washing performance and indigo backstaining for trichoderma reesei and chrysosporium lucknowense commercial cellulase preparations on a 'test-tube scale'. c. lucknowense preparation demonstrated a higher potential in the denim biostoning process. the performance of four purified cellulases (two endoglucanases and two cellobiohydrolases) from c. lucknowense on cotton textiles was assayed, and the key enzyme (endoglucanase with a molecular weight o ... | 2001 | 11500217 |
| biological degradation of 2,4,6-trinitrotoluene. | nitroaromatic compounds are xenobiotics that have found multiple applications in the synthesis of foams, pharmaceuticals, pesticides, and explosives. these compounds are toxic and recalcitrant and are degraded relatively slowly in the environment by microorganisms. 2,4,6-trinitrotoluene (tnt) is the most widely used nitroaromatic compound. certain strains of pseudomonas and fungi can use tnt as a nitrogen source through the removal of nitrogen as nitrite from tnt under aerobic conditions and the ... | 2001 | 11527999 |
| diversity of micro-fungi in an antarctic dry valley. | the fungal microflora of a dry valley in southern victoria land near mcmurdo sound, antarctica, was investigated. samples were collected from introduced objects such as a mummified penguin and spent chewing tobacco in addition to the sparse soil found in rock fissures, isolated moss colonies, shoreline deposit materials, caco3 precipitates, and microbial mat debris obtained from the frozen surface of the lake in the basin of taylor valley. using conventional media and techniques, all collectio ... | 1991 | 11539818 |
| nutrient recovery and biodegradation of inedible tomato plant residues by activated sludge cultures and phanerochaete chrysosporium. | the biodegradation of inedible biomass and the recovery of nutrients from hydroponically grown tomato plant material were investigated under various growth conditions of activated sludge and the fungus phanerochaete chrysosporium. the experiments were carried out in shaker flasks at three incubation temperatures (25 degrees c, 40 degrees c, and 60 degrees c for the activated sludge and 25 degrees c, 40 degrees c, and 50 degrees c for the fungi) with heat-pretreated samples at 150 degrees c for 3 ... | 1998 | 11540465 |
| pcb biodegradation in aged contaminated soil: interactions between exogenous phanerochaete chrysosporium and indigenous microorganisms. | this work investigated whether the interaction between the white-rot fungus phanerochaete chrysosporium and indigenous microorganisms could enhance polychlorinated biphenyl (pcb) removal from historically contaminated soil in aerobic microcosms. the pcb mixture was composed mainly of 14% tri-, 20% tetra-, 9% penta-, 17% hexa-, 26% hepta-, 11% octa-, and 3% nona-chlorobiphenyl (cb) congeners, determined by gc/ms. the fungus, which was grown on sugarcane bagasse and added via this solid substrate, ... | 2001 | 11545344 |
| treatment of wastewater sludge by liquid state bioconversion process. | this study was conducted to evaluate the effect of an eminent decay fungus, phanerocheate chrysosporium of organic residues on wastewater sludge for its improvement through decomposition and separation of waste particles by liquid state bioconversion (lsb). the effect of fungal treatment was compared to uninoculated (control) at three different harvests 7, 14 and 21 days after inoculation (dai). the observed results showed that the weight loss and solid content of wastewater sludge were signific ... | 2001 | 11545349 |
| fungicidal activity of beta-thujaplicin analogues. | the fungicidal activity of analogues of beta-thujaplicin, a natural product responsible for the durability of heartwood of several cupressaceous trees, was investigated in vitro on the growth of different white and brown rot fungi involved in wood biodegradation, coriolus versicolor, phanerochaete chrysosporium, poria placenta and gloephyllum trabeum. the study shows that 2-hydroxycyclohepta-2,4,6-trienone (tropolone), easily prepared according to a literature procedure, possesses interesting fu ... | 2001 | 11561410 |
| solid-state production of lignin peroxidase (lip) and manganese peroxidase (mnp) by phanerochaete chrysosporium using steam-exploded straw as substrate. | in the used media mainly consisting of steam-exploded wheat straw, the straw, which could replace expensive veratryl alcohol, might act not only as nutrient, but also as inducer of lignin enzymes. the activities of the enzymes lignin peroxidase (lip) and manganese peroxidase (mnp) in solid-state fermentation (ssf) were far higher than in submerged fermentation (smf). under optimal conditions of ssf, the maximum activities of the enzymes lip and mnp were 2600 and 1375 u/l, respectively. thus, thi ... | 2001 | 11563706 |
| glyoxal oxidase of phanerochaete chrysosporium: its characterization and activation by lignin peroxidase. | glyoxal oxidase (glox) is an extracellular h2o2-generating enzyme produced by ligninolytic cultures of phanerochaete chrysosporium. the production, purification, and partial characterization of glox from agitated cultures are described here. high-oxygen levels are critical for glox production as for lignin peroxidase. glox purified by anion-exchange chromatography appears homogeneous by nadod-so4/page (molecular mass = 68 kda). however, analysis by isoelectric focusing indicates two major bands ... | 1990 | 11607073 |
| crystal structure of lignin peroxidase. | the crystal structure of lignin peroxidase (lip) from the basidiomycete phanerochaete chrysosporium has been determined to 2.6 a resolution by usine multiple isomorphous replacement methods and simulated annealing refinement. of the 343 residues, residues 3-335 have been accounted for in the electron density map, including four disulfide bonds. the overall three-dimensional structure is very similar to the only other peroxidase in this group for which a high-resolution crystal structure is avail ... | 1993 | 11607355 |
| fungal degradation of recalcitrant nonphenolic lignin structures without lignin peroxidase. | lignin peroxidases (lips) are likely catalysts of ligninolysis in many white-rot fungi, because they have the unusual ability to depolymerize the major, recalcitrant, non-phenolic structures of lignin. some white-rot fungi have been reported to lack lip when grown on defined medium, but it is not clear whether they exhibit full ligninolytic competence under these conditions. to address this problem, we compared the abilities of a known lip producer, phanerochaete chrysosporium, with those of a r ... | 1994 | 11607502 |
| production and characterization of recombinant phanerochaete chrysosporium cellobiose dehydrogenase in the methylotrophic yeast pichia pastoris. | the hemoflavoenzyme cellobiose dehydrogenase (cdh) from the white-rot fungus phanerochaete chrysosporium has been heterologously expressed in the methylotrophic yeast pichia pastoris. after 4 days of cultivation in the induction medium, the expression level reached 1800 u/l (79 mg/l) of cdh activity, which is considerably higher than that obtained previously for wild-type cdh (wtcdh) and recombinant cdh (rcdh) produced by p. chrysosporium. analysis with sds-page and coomassie brilliant blue (cbb ... | 2001 | 11676020 |
| roles of two white-rot basidiomycete fungi in decolorisation and detoxification of olive mill waste water. | a phanerochaete chrysosporium strain was isolated from moroccan olive mill waste water (omw) and its ability to degrade omw in different culture conditions was investigated and compared to that of pleurotus ostreatus. the results indicated that ph. chrysosporium isolate is more efficient than pl. ostreatus in decolorising and detoxifying omw in the presence of added nutrients. ph. chrysosporium is able to remove more than 50% of the colour and phenols from omw within 6 days of incubation, wherea ... | 2001 | 11693925 |
| engineering of the h2o2-binding pocket region of a recombinant manganese peroxidase to be resistant to h2o2. | the manganese peroxidase produced by phanerochaete chrysosporium, which catalyzes the oxidation of mn(2+) to mn(3+), is easily inactivated by the hydrogen peroxide (h2o2) presented in the reaction. we attempted to increase h2o2 resistance by the conformational stabilization around the h2o2-binding pocket. based on its structural model, engineering of oxidizable met273 located near the pocket to a non-oxidizable leu showed a great improvement. furthermore, after treatment at 1 mm h2o2 where the w ... | 2001 | 11734216 |
| 5-demethylovalicin, as a methionine aminopeptidase-2 inhibitor produced by chrysosporium. | 5-demethylovalicin was isolated from the fermentation broth chrysosporium lucknowense and the structure was identified by spectroscopic methods. 5-demethylovalicin inhibited the recombinant human metap-2 (ic(50)=17.7 nm) and the growth of human umbilical vein endothelial cells (huvec; ic(50)=100 nm) in cell proliferation assay without cytotoxicity on the transformed and cancer cell lines. | 2002 | 11738620 |
| family 7 cellobiohydrolases from phanerochaete chrysosporium: crystal structure of the catalytic module of cel7d (cbh58) at 1.32 a resolution and homology models of the isozymes. | cellobiohydrolase 58 (cel7d) is the major cellulase produced by the white-rot fungus phanerochaete chrysosporium, constituting approximately 10 % of the total secreted protein in liquid culture on cellulose. the enzyme is classified into family 7 of the glycosyl hydrolases, together with cellobiohydrolase i (cel7a) and endoglucanase i (cel7b) from trichoderma reesei. like those enzymes, it catalyses cellulose hydrolysis with net retention of the anomeric carbon configuration. the structure of th ... | 2001 | 11743726 |
| rheological behavior of phanerochaete chrysosporium broth during lignin degradation. | rheological behavior of a lignin-degrading culture of phanerochaete chrysosporium cdbb-h-298 was determined. rheological characteristics revealed a newtonian behavior in the culture medium without fungi, and a non-newtonian dilatant behavior with fungi. in addition, the rheological behavior of the lignin-containing culture medium was complex, and changed from dilatant to pseudoplastic. during fungal growth without lignin in kirk media rheological behavior exhibited a parabolic profile, and thixo ... | 2001 | 11759909 |
| human hair colonizing fungi in water sediments of india. | out of 144 samples of water sediments, 183 isolates belonging to 9 genera and 22 species were isolated. fifty-nine isolates of acremonium, 26 of chrysosporium indicum, 22 of chrysosporium keratinophilum, 17 of malbranchea sp. and 10 of microsporum gypseum were recovered. acremonium implicatum, chrysosporium georgii, chrysosporium xerophilum and geomyces pannorum were reported for the first time from india. | 2001 | 11761148 |
| 14-(2 ',3 ',5 '-trihydroxyphenyl)tetradecan-2-ol, a novel acetylcholinesterase inhibitor from chrysosporium sp. | | 2001 | 11776443 |
| studies on the decolourisation of an artificial textile-effluent by white-rot fungi in n-rich and n-limited media. | coriolopsis gallica and phanerochaete chrysosporium were selected for their potential ability to degrade five dyes in an artificial effluent. degradation experiments were carried out in n-rich (c:n ratio 11.6:1) and n-limited (116:1) conditions at an effluent concentration of 100 mg l(-1). p. chrysosporium decolourised 53.6% of the effluent in n-rich conditions and 48% in n-limited conditions. c. gallica decolourised 80.7% in n-rich conditions and 86.9% in n-limited conditions. nitrogen suppleme ... | 2001 | 11778898 |
| crystal structure of the flavoprotein domain of the extracellular flavocytochrome cellobiose dehydrogenase. | cellobiose dehydrogenase (cdh) participates in the degradation of cellulose and lignin. the protein is an extracellular flavocytochrome with a b-type cytochrome domain (cyt(cdh)) connected to a flavodehydrogenase domain (dh(cdh)). dh(cdh) catalyses a two-electron oxidation at the anomeric c1 position of cellobiose to yield cellobiono-1,5-lactone, and the electrons are subsequently transferred from dh(cdh) to an acceptor, either directly or via cyt(cdh). here, we describe the crystal structure of ... | 2002 | 11786022 |
| solid-state production of biopulp by phanerochaete chrysosporium using steam-exploded wheat straw as substrate. | a novel material for biopulp-making, steam-exploded wheat straw (sews), was studied. during the steam explosion process, the hemicellulose was partly degraded and became water-soluble sugar as the carbon resource of the chosen microbe growth, and compared with non-sews, the degradation amount of cellulose decreased and the degradation amount of lignin increased for the fermented steam-exploded wheat straw (fsews) cultured with phanerochaete chrysosporium me-446. under the optimum conditions of s ... | 2002 | 11800492 |
| calnexin overexpression increases manganese peroxidase production in aspergillus niger. | heme-containing peroxidases from white rot basidiomycetes, in contrast to most proteins of fungal origin, are poorly produced in industrial filamentous fungal strains. factors limiting peroxidase production are believed to operate at the posttranslational level. in particular, insufficient availability of the prosthetic group which is required for peroxidase biosynthesis has been proposed to be an important bottleneck. in this work, we analyzed the role of two components of the secretion pathway ... | 2002 | 11823227 |
| biodegradation of polyvinyl alcohol by phanerochaete chrysosporium after pretreatment with fenton's reagent. | polyvinyl alcohol (pva) solutions (bp05 and bf17; 5.0%, wt v(-1)) were degraded by a combination of chemical (fenton's reagent) and fungal (phanerochaete chrysosporium) treatments. the overall degradations of bp05 and bf17 were 74.4 and 72.8%, respectively, as determined by chemical oxygen demand (cod) analysis, and 63.7% and 57.7%, respectively, as determined by total organic carbon (toc) analysis. increased retention times and changes in the intensity of the pva peaks on gel permeation chromat ... | 2002 | 11846267 |
| iron-regulated proteins in phanerochaete chrysosporium and lentinula edodes: differential analysis by sodium dodecyl sulfate polyacrylamide gel electrophoresis and two-dimensional polyacrylamide gel electrophoresis profiles. | sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page) and two-dimensional gel electrophoresis (2-de) were used to identify iron-responsive proteins in the white-rot species (phanerochaete chrysosporium and lentinula edodes), by comparing the differential patterns of cellular and membrane proteins obtained from iron-sufficient and iron-deficient mycelia. six cellular proteins induced by iron restriction have been observed in sds-page for p. chrysosporium and twelve for l. edodes. i ... | 2002 | 11870778 |
| lignin peroxidase oxidation of veratryl alcohol: effects of the mutants h82a, q222a, w171a, and f267l. | the site-directed mutations h82a and q222a (residues near the heme access channel), and w171a and f267l (residues near the surface of the protein) were introduced into the gene encoding lignin peroxidase (lip) isozyme h8 from phanerochaete chrysosporium. the variant enzymes were produced by homologous expression in p. chrysosporium, purified to homogeneity, and characterized by kinetic and spectroscopic methods. the molecular masses, the pis, and the uv-vis absorption spectra of the ferric and o ... | 2002 | 11876658 |
| pulmonary colonization by chrysosporium zonatum associated with allergic inflammation in an immunocompetent subject. | we report a case of noninvasive pulmonary disease due to chrysosporium zonatum in an immunocompetent male. the fungus colonized an existing tuberculous cavity and was isolated from transbronchial lavage fluid and from a percutaneous aspiration specimen. the disease was accompanied by the unusual feature of an allergic reaction. the fungus ball was successfully treated by intracavitary administration of amphotericin b. c. zonatum is the anamorph of the heterothallic ascomycete uncinocarpus orissi ... | 2002 | 11880456 |
| biological remediation of explosives and related nitroaromatic compounds. | nitroaromatics form an important group of recalcitrant xenobiotics. only few aromatic compounds, bearing one nitro group as a substituent of the aromatic ring, are produced as secondary metabolites by microorganisms. the majority of nitroaromatic compounds in the biosphere are industrial chemicals such as explosives, dyes, polyurethane foams, herbicides, insecticides and solvents. these compounds are generally recalcitrant to biological treatment and remain in the biosphere, where they constitut ... | 2002 | 11885418 |
| biosorption of lead by phanerochaete chrysosporium in the form of pellets. | the growth of phanerochaete chrysosporium(atcc 24725) in pellets was influenced by culture time, medium ph, c/n, surfactant concentration, spore number in inoculum, and shaking rate. the removal of pb2+ from aqueous solution by this kind of mycelial pellets was studied. the results indicated that many factors affected biosorption. these factors included ph, pb2+ concentration, co-ion, adsorption time, and chemical pretreatments of biomass. under optimum biosorption conditions(ph 4.5, 27 degrees ... | 2002 | 11887305 |
| fungi isolated from skins and pens of healthy animals in nigeria. | the mycoflora of 220 skin scrapings, hair, nail samples and pens' materials of apparently healthy animals including cows, sheep, goats, rabbits, pigs and dogs were determined. twenty eight species of fungi belonging to ten genera were recovered. chrysosporium spp. were the most common and c. keratinophilum was recovered from all animals. dermatophytes which are known causal agents of dermatophytosis were also isolated in different frequencies (microsporum gypseum, m. canis, trichophyton mentagro ... | 2002 | 11913761 |
| [effect of cyclodextrins on fungal degradation of fluorene]. | the purpose of this study was to improve the bioavailability of fluorene (pah) by the use of complexing agents, cyclodextrins. the biodegradation tests were performed in liquid medium batches; fluorene was quantified by hplc. experimental results showed the enhancement of fluorene degradation by penicillium italicum and phanerochaete chrysosporium in the presence of branched cyclodextrins. | 2001 | 11924508 |
| investigation of the response of wood-rotting fungi to copper stress by size-exclusion chromatography and capillary zone electrophoresis with icp ms detection. | a method based on the coupling of size-exclusion chromatography (sec) with inductively coupled plasma mass spectrometry (icp ms) was developed for screening the changes in the bioligand composition of wood-rotting fungi as a function of their exposure to copper stress. strains of four different species of wood-rotting fungi: phanerochaete chrysosporium, schizophyllum commune, daedalea quercina and pleurotus ostreatus were examined. only one, namely ph. chrysosporium, showed any significant diffe ... | 2002 | 11939533 |
| kinetics of inter-domain electron transfer in flavocytochrome cellobiose dehydrogenase from the white-rot fungus phanerochaete chrysosporium. | the pre-steady-state kinetics of inter-domain electron transfer in the extracellular flavocytochrome cellobiose dehydrogenase from phanerochaete chrysosporium was studied using various values of ph and substrate concentration. monitoring at the isosbestic point of each prosthetic group indicated that the reductive half-reactions of flavin and haem were biphasic and monophasic respectively. when the observed rates of the flavin and haem reactions were plotted against substrate concentration, the ... | 2002 | 11939907 |
| responses of coptotermes formosanus and reticulitermes flavipes (isoptera: rhinotermitidae) to three types of wood rot fungi cultured on different substrates. | this study examined the responses of two termite species, the formosan subterranean termite, coptotermes formosanus shiraki, and the eastern subterranean termite, reticulitermes flavipes (kollar), to three types of wood decay fungi: a brown rot fungus, gloeophyllum trabeum (persoon: fries) murrill; a white rot fungus, phanerochaete chrysosporium burdsall; and a litter rot fungus, marasmiellus troyanus (murrill) singer. we also examined the responses of termites to these three types of fungi grow ... | 2002 | 11942746 |
| biodegradation of volatile organic compounds by five fungal species. | five fungal species, cladosporium resinae (atcc 34066), cladosporium sphaerospermum (atcc 200384), exophiala lecanii-corni (cbs 102400), mucor rouxii (atcc 44260), and phanerochaete chrysosporium (atcc 24725), were tested for their ability to degrade nine compounds commonly found in industrial off-gas emissions. fungal cultures inoculated on ceramic support media were provided with volatile organic compounds (vocs) via the vapor phase as their sole carbon and energy sources. compounds tested inc ... | 2002 | 11956756 |
| reactions of the class ii peroxidases, lignin peroxidase and arthromyces ramosus peroxidase, with hydrogen peroxide. catalase-like activity, compound iii formation, and enzyme inactivation. | the reactions of the fungal enzymes arthromyces ramosus peroxidase (arp) and phanerochaete chrysosporium lignin peroxidase (lip) with hydrogen peroxide (h(2)o(2)) have been studied. both enzymes exhibited catalase activity with hyperbolic h(2)o(2) concentration dependence (k(m) approximately 8-10 mm, k(cat) approximately 1-3 s(-1)). the catalase and peroxidase activities of lip were inhibited within 10 min and those of arp in 1 h. the inactivation constants were calculated using two independent ... | 2002 | 11983689 |
| biodecolourization of azo and triphenylmethane dyes by dichomitus squalens and phlebia spp. | nine white-rot fungal strains were screened for biodecolourization of brilliant green, cresol red, crystal violet, congo red and orange ii. dichomitus squalens, phlebia fascicularia and p. floridensis decolourized all of the dyes on solid agar medium and possessed better decolourization ability than phanerochaete chrysosporium when tested in nitrogen-limited broth medium. | 2002 | 11986919 |
| utilisation of lignocellulosic wastes for lignin peroxidase production by semi-solid-state cultures of phanerochaete chrysosporium. | in the present work, the production of ligninolytic enzymes by semi-solid-state cultures of phanerochaete chrysosporium bkm-f-1767 (atcc 24725), employing different lignocellulosic wastes as support, was investigated. the waste materials employed were grape seeds, wheat straw and wood shavings. maximum lignin peroxidase activities of 1,620 +/- 123 u/l,364 +/- 35 u/l and 571 +/- 42 u/l were attained, respectively. nevertheless, low manganese-dependent peroxidase activities were found, being insig ... | 2001 | 11995821 |
| oxygen reduction by cellobiose oxidoreductase: the role of the haem group. | we have used optical and electron paramagnetic spectroscopy to study the flavohaem enzyme cellobiose oxidoreductase (cbor) from phanerochaete chrysosporium. we have examined redox cycles of the enzyme in which the oxidation of cellobiose to cellobionolactone is coupled to the reduction of oxygen. during turnover flavin can reduce oxygen with one electron to produce superoxide or two electrons to produce hydrogen peroxide. addition of superoxide dismutase significantly extended the time courses o ... | 2002 | 11997012 |
| occurrence of keratinophilic fungi and dermatophytes on domestic birds in nigeria. | feathers, nails and beaks of one hundred and twenty common birds in nigeria, chicken [50], ducks [20], turkeys [15] and pigeons [35], were examined using the soil plate technique for their mycoflora. 15 species of fungi were recovered and they belong to the genera chrysosporium, trichophyton, microsporum, aspergillus, fusarium, mucor rhizopus, penicillium and trichoderma. microsporum gypseum was the species most frequently isolated (35% of the samples). the most common genus was chrysosporium an ... | 2002 | 12000131 |
| bio-degradation of lignin in olive pomace by freshly-isolated species of basidiomycete. | the solid waste (pomace) from olive oil processing cannot be used directly as an animal feed, but it was thought that an appropriate series of fermentations might improve its nutritional value. as a first step, typical samples of pomace were subjected, after an alkaline pre-treatment, to delignification by phanerochaete chrysosporium (atcc 19343), oxysporus sp., schizophyllum commune, hyphoderma sp. or ganoderma sp.; the last four species being freshly isolated from decaying wood collected in a ... | 2002 | 12003313 |
| use of steam explosion liquor from sugar cane bagasse for lignin peroxidase production by phanerochaete chrysosporium. | the possibility of using two by-products of the sugar cane industry, molasses and bagasse steam explosion liquor (sel), for lignin peroxidase (lip) production by phanerochaete chrysosporium was investigated. for comparison, the fungus was initially cultivated in synthetic media containing either glucose, sucrose, xylose, or xylan as sole carbon sources. the effect of veratryl alcohol (va) was also investigated in relation to the enzyme activity levels. results showed that sucrose was not metabol ... | 2002 | 12018256 |
| cellobiose quinone oxidoreductase from the white rot fungus phanerochaete chrysosporium is produced by intracellular proteolysis of cellobiose dehydrogenase. | the fungus phanerochaete chrysosporium was grown in a 10-l automatic fermenter using cellobiose as carbon source to monitor the induction of cellobiose dehydrogenase (cdh) and cellobiose quinone oxidoreductase (cbq) enzymes, and to search for tentative cbq and cdh genes and their transcriptional products. after 24 h of induction, cdh was detected in the culture supernatant and a protein was recognized by a specific anti-cdh polyclonal antibody in the sonicated biomass. northern blot experiments ... | 2002 | 12031479 |
| effect of pollutants on the ergosterol content as indicator of fungal biomass. | ergosterol content was determined in 20 white-rot fungi isolates and the values ranged from 2380 to 13060 microg g(-1) fungal biomass. significant changes of ergosterol content according the physiological stage for bjerkandera adusta 4312 and coriolopsis gallica 8260 were found, showing the highest values during the stationary phase. however, in the case of phanerochaete chrysosporium 3642, no changes were detected during growth. the effect of pollutants, such as heavy metals and fungicides, on ... | 2002 | 12031573 |
| soil saprotrophic microfungi associated with roots of calamagrostis epigeios on an abandoned deposit of toxic waste from smelter factory processing pyrite raw materials. | saprotrophic microfungi associated with roots of calamagrostis epigeios growing in industrial waste contaminated with high concentrations of heavy metals (manganese, iron, zinc) were isolated on three different media. the isolates were obtained from three plots colonized by plant cenoses, representing three different succession series of plant cover: initial, intermediate, and advanced. a total of 60 species of saprotrophic microfungi were found in the roots during three samplings. in addition, ... | 2001 | 12032621 |
| biodegradation of a polymeric dye in a pulsed bed bioreactor by immobilised phanerochaete chrysosporium. | an immobilised fungal bioreactor operated with pulsation of the gas-phase is proposed and operated at variable conditions for the continuous biological degradation of complex structures. in order to test the system, a hardly biodegradable dye (poly r-478) was selected as a model compound and phanerochaete chrysosporium as the ligninolytic fungus. high percentages of decolourisation--between 65% and 80%--under optimal conditions were achieved. moreover, the system proved to have high stability wi ... | 2002 | 12044089 |
| removal of surfactant solubilized polycyclic aromatic hydrocarbons by phanerochaete chrysosporium in a rotating biological contactor reactor. | white rot fungi can oxidize surfactant solubilized polycyclic aromatic hydrocarbons (pah). the objective of this study was to evaluate the performance of immobilized white rot fungus, phanerochaete chrysosporium, to remove surfactant tween 80 solubilized pah i.e. phenanthrene, pyrene and benzo(alpha)pyrene in a rotating biological contactor (rbc) reactor. results indicated that the immobilized p. chrysosporium in the rbc reactor system in continuous operation could effectively remove the three t ... | 2002 | 12044552 |
| biotransformation of linear alkylbenzene sulfonate (las) by phanerochaete chrysosporium: oxidation of alkyl side-chain. | the white rot fungus phanerochaete chrysosporium, which generally mineralizes substituted aromatics to co2, transformed linear alkylbenzene sulfonate (las) surfactants mainly at their alkyl side chain. degradation of las was evidenced by a zone of clearing on las-containing agar plates and colorimetric analysis of liquid cultures. disappearance of las was virtually complete within 10 days in low nitrogen (2.4 mm n), high nitrogen (24 mm n) and malt extract (me) liquid media. after 5 days of incu ... | 2001 | 12051650 |
| immunohistochemical characterization of tuberculous and non-tuberculous lesionsin naturally infected european badgers (meles meles). | a panel of species cross-reactive antibodies was established for the immunohistochemical labelling of phagocytic and lymphoid cells in formalin-fixed normal badger tissues. these reagents were used to investigate the immunopathogenesis of both tuberculous and non-tuberculous granulomas in badgers. in normal badger tissues, antisera specific for the cd79a and cd79b epitopes strongly labelled follicular b lymphocytes and plasma cells in lymph nodes, bronchus-associated lymphoid tissue and peyer's ... | 2002 | 12056773 |
| causative agents of nosocomial mycoses. | in the last few years mycoses have been caused by fungi formerly considered to be harmless for humans. they cause diseases of plants and insects; some of them are also used in the industry. they are now usually called "emerging fungi". we investigated this flora with respect to their potential to cause infections in hospitals. these fungi are present in the air, on medical objects and instrumentation, in the respiratory tract and on the hands of hospital staff; other sources have been identified ... | 2002 | 12058386 |
| chrysosporium anamorph of nannizziopsis vriesii associated with fatal cutaneous mycoses in the salt-water crocodile (crocodylus porosus). | the chrysosporium anamorph of nannizziopsis vriesii, recently identified as the cause of cutaneous infections in chameleons and brown tree snakes, was associated with skin infections and deaths in salt-water crocodile (crocodylus porosus) hatchlings on two separate occasions 3 years apart. in all, 48 animals died from the infection. all hatchlings came from the same farm in northern queensland, australia. | 2002 | 12058727 |
| decolorization of synthetic textile dyes by lignin peroxidase of phanerochaete chrysosporium. | neem hull waste (containing a high amount of lignin and other phenolic compounds) was used for lignin peroxidase production by phanerochaete chrysosporum under solid-state fermentation conditions. maximum decolorization achieved by partially purified lignin peroxidase was 80% for porocion brilliant blue hgr, 83 for ranocid fast blue, 70 for acid red 119 and 61 for navidol fast black msrl. the effects of different concentrations of veratryl alcohol, hydrogen peroxide, enzyme and dye on the effici ... | 2002 | 12094739 |
| analysis of 5' upstream regulatory sequences of lignin peroxidase(lip) genes of phanerochaete chrysosporium. | phanerochaete chrysosporium yields exocellular isozymes of lignin peroxidases and mn peroxidases which are able to degrade lignin. in order to investigate the regulatory mechanism of lip genes at transcriptional level, 6 dna fragments subcloned from 5' upstream sequences of lip genes (glg3 and glg6) were obtained, then gel mobility shift assay was carried out to screen dna fragment(s) with ability to be bound specifically by proteins isolated from p.chrysosporium mycelia. the results showed that ... | 1999 | 12110933 |
| degradation of chlorophenols by phanerochaete chrysosporium: effect of 3,4-dichlorophenol on extracellular peroxidase activities. | extracellular peroxidases play an important role in the degradation of chlorophenols by phanerochaete chrysosporium. depending on the moment of 3,4-dichlorophenol addition, the production of lignin peroxidase and manganese peroxidase in c-limited agitated cultures was affected in opposite ways. in cultures that received 3,4-dichlorophenol at the time of inoculation, fungal growth was reduced and peroxidases were not produced, whereas peroxidase activities were stabilized after 3,4-dichlorophenol ... | 2002 | 12111159 |
| direct surface analysis of fungal species by matrix-assisted laser desorption/ionization mass spectrometry. | in this study various methods of sample preparation and matrices were investigated to determine optimum collection and analysis criteria for fungal analysis by matrix-assisted laser desorption/ionization mass spectrometry (maldi-ms). intact spores and/or hyphae of aspergillus niger, rhizopus oryzae, trichoderma reesei and phanerochaete chrysosporium were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tofms). the fungal samples were applied to the ... | 2002 | 12112615 |
| chemical stress-responsive genes from the lignin-degrading fungus phanerochaete chrysosporium exposed to dibenzo-p-dioxin. | the stress-responsive genes expressed against the exogenous addition of dibenzo-p-dioxin from the lignin-degrading basidiomycete, phanerochaete chrysosporium, were determined utilizing a differential display reverse transcription-pcr technique. six cdna fragments, exhibiting a high homology with various proteins from other microorganisms, were identified via a blast search; that is, nadh-ubiquinone oxidoreductase (nuo), atp/adp carrier, uric acid-xanthine permease, manganese superoxide dismutase ... | 2002 | 12113937 |
| queenslandon, a new antifungal compound produced by chrysosporium queenslandicum: production, isolation and structure elucidation. | | 2002 | 12139022 |
| degradation of 4-chlorophenol by the white rot fungus phanerochaete chrysosporium in free and immobilized cultures. | 4-chlorophenol (4-cp) degradation was investigated by suspended and immobilized phanerochaete chrysosporium conducted in static and agitated cultures. the best results were achieved when experiment was carried out in a rotating biological contactor instead of an erlenmeyer flask, for both batch degradation and repeated batch degradation. the relative contribution of lignin peroxidase (lip) versus manganese peroxidase (mnp) to the 4-cp degradation by p. chrysosporium was investigated. 4-cp degrad ... | 2002 | 12139330 |
| incidence of dermatophytes and cyclohexamide resistant fungi on healthy children hairs and nails in nurseries. | in order to estimate the prevalence of dermatophytes and other fungi on healthy children hairs and nails, 92 hair samples and 85 nail samples (groups of 10 finger nails from each child) were collected from 5 nurseries (children aged 9 months up to 4 years) in assiut city. from hair samples 22 species were collected, trichophyton (2 species) and microsporum (2 species) were the only recovered dermatophytes in addition to well known keratinophilic genus chrysosporium (4 species). from nail samples ... | 2002 | 12206316 |
| degradation of keratin substrates by fungi isolated from sewage sludge. | four fungal species including two dermatophytes and two saprophytes were isolated from sewage sludge samples at basrah (iraq) they were tested for their degradative ability towards three types of keratin substrates (human hair, chicken feathers and wool). the rate of keratin degradation was expressed as weight loss over three weeks of incubation using a liquid culture medium. human hair had the highest degradation rate by colonization of chrysosporium pannicola and microsporum gypseum at a rate ... | 2002 | 12206319 |
| identification and heterologous expression of the cytochrome p450 oxidoreductase from the white-rot basidiomycete coriolus versicolor. | a cdna encoding cytochrome p450 oxidoreductase (cpr) from the lignin-degrading basidiomycete coriolus versicolor was identified using rt-pcr. the full-length cdna consisted of 2,484 nucleotides with a poly(a) tail, and contained an open reading frame. the g+c content of the cdna isolated was 60%. a deduced protein contained 730 amino acid residues with a calculated molecular weight of 80.7 kda. the conserved amino acid residues involved in functional domains such as fad-, fmn-, and nadph-binding ... | 2002 | 12226721 |
| relationship of chemical structures of textile dyes on the pre-adaptation medium and the potentialities of their biodegradation by phanerochaete chrysosporium. | azo dye derivatives of azobenzene constitute the largest group of dyes used in the textile industry and possess recalcitrant chemical groups, such as those of azo and sulphonic acid. some microorganisms are able to degrade these aromatic compounds. in the present work, decolourisation of culture media containing azo dyes by the ligninolytic fungus phanerochaete chrysosporium was achieved under nitrogen-limited conditions. the dyes used in the study are derivatives of meta- or para-aminosulphonic ... | 2002 | 12234010 |
| molecular analysis of arylalcohol dehydrogenase of coriolus versicolor expressed against exogenous addition of dibenzothiophene derivatives. | using the differential display reverse-transcriptional polymerase chain reaction (ddrt-pcr) technique, several cdna fragments were isolated as chemical stress responsive genes from the white-rot basidiomycete, coriolus versicolor, exposed to either 4-methyldibenzothiophene-5-oxide (4mdbto) or dibenzothiophene-5-oxide (dbto). a database search on deduced amino acid sequences of cdnas revealed that they showed a high similarity with various proteins from other organisms. these results strongly sug ... | 2002 | 12362404 |
| biodegradation of aromatic compounds by white rot and ectomycorrhizal fungal species and the accumulation of chlorinated benzoic acid in ectomycorrhizal pine seedlings. | the capability of different white rot (wr, heterobasidion annosum, phanerochaete chrysosporium, trametes versicolor) and ectomycorrhizal (ecm, paxillus involutus, suillus bovinus) fungal species to degrade different aromatic compounds and the absorption of 3-chlorobenzoic acid (3-cba) by ecm pine seedlings was examined. the effect of aromatic compounds on the fungal biomass development varied considerably and depended on (a) the compound, (b) the external concentration, and (c) the fungal specie ... | 2002 | 12363308 |
| production of ligninolytic enzymes for dye decolorization by cocultivation of white-rot fungi pleurotus ostreatus and phanerochaete chrysosporium under solid-state fermentation. | lignocellulosic wastes such as neem hull, wheat bran, and sugarcane bagasse, available in abundance, are excellent substrates for the production of ligninolytic enzymes under solid-state fermentation by white-rot fungi. a ligninolytic enzyme system with high activity showing enhanced decomposition was obtained by cocultivation of pleurotus ostreatus and phanerochaete chrysosporium on combinations of lignocellulosic waste. among the various substrate combinations examined, neem hull and wheat bra ... | 2002 | 12396115 |
| heavy metal removal by biosorption using phanerochaete chrysosporium. | biosorption using microbial cells as adsorbents is being seen as a cost-effective method for the removal of heavy metals from wastewaters. biosorption studies with phanerochaete chrysosporium were performed for copper (ii), lead (ii), and cadmium (ii) to evaluate the effectiveness and to optimize the operational parameters using response surface methodology. the operational parameters chosen were initial metal ion concentration, ph, and biosorbent dosage. using this method, the metal removal cou ... | 2002 | 12396126 |
| transcript analysis of genes encoding a family 61 endoglucanase and a putative membrane-anchored family 9 glycosyl hydrolase from phanerochaete chrysosporium. | phanerochaete chrysosporium cellulase genes were cloned and characterized. the cel61a product was structurally similar to fungal endoglucanases of glycoside hydrolase family 61, whereas the cel9a product revealed similarities to thermobifida fusca cel9a (e4), an enzyme with both endo- and exocellulase characteristics. the fungal cel9a is apparently a membrane-bound protein, which is very unusual for microbial cellulases. transcript levels of both genes were substantially higher in cellulose-grow ... | 2002 | 12406778 |
| enzyme production activity of phanerochaete chrysosporium and degradation of pentachlorophenol in a bioreactor. | lignin peroxidase production by a white rot fungus, phanerochaete chrysosporium, was experimentally investigated using a batch system and a reactor system with various carriers. immobilization of mycelia cell culture was more effective in promoting cell growth and lignin peroxidase production compared to conventional stationary liquid culture. biostage carrier, commonly used for biochemical treatment in a fluidized bed disposal system, greatly improved production of lignin peroxidase up to 8.1 u ... | 2002 | 12418647 |
| starch-based plastic polymer degradation by the white rot fungus phanerochaete chrysosporium grown on sugarcane bagasse pith: enzyme production. | in this study, starch metabolites and enzymes were determined during starch-based plastic polymer biodegradation by the white rot fungus phanerochaete chrysosporium, grown in sugarcane bagasse pith in tubular reactors. various metabolites, amylase, ligninase and cellulase production were measured during p. chrysosporium growth on sugarcane bagasse pith with added glucose and starch polymer. on-line respirometric analyses followed during 32 days confirmed the p. chrysosporium capability of growin ... | 2003 | 12421000 |
| did earthworms contribute to the parasitic evolution of dermatophytes? | the survival of dermatophyte species in the gut of four species of earthworms was studied by feeding the fungi to the earthworms. recovery of the dermatophyte species in culture from the guts was only possible for microsporum gypseum and chrysosporium keratinophilum. in the light of these findings, we presume that earthworms could have influenced the parasitic evolution of certain dermatophytes. | 2002 | 12421289 |
| production of manganese-dependent peroxidase in a new solid-state bioreactor by phanerochaete chrysosporium grown on wood shavings. application to the decolorization of synthetic dyes. | the production of manganese-dependent peroxidase (mnp) by phanerochaete chrysosporium in a new solid-state bioreactor, the immersion bioreactor, operating with lignocellulosic waste, such as wood shavings, was investigated. maximum mnp and lignin peroxidase (lip) activity of 13.4 and 8.48 mukat/l were obtained, respectively. the in vitro decolorization of several synthetic dyes by the extracellular liquid produced in the above-mentioned bioreactor (containing mainly mnp) was carried out and its ... | 2002 | 12422521 |
| influence of temperature, water activity and ph on growth of some xerophilic fungi. | the combined effects of water activity (aw), ph and temperature on the germination and growth of seven xerophilic fungi important in the spoilage of baked goods and confectionery were examined. eurotium rubrum, e. repens, wallemia sebi, aspergillus penicillioides, penicillium roqueforti, chrysosporium xerophilum and xeromyces bisporus were grown at 25, 30 and 37 degrees c on media with ph values of 4.5, 5.5, 6.5 and 7.5 and a range of water activities (aw) from 0.92 to 0.70. the aw of the media ... | 2003 | 12423914 |
| characterization of manganese-dependent peroxidase isoenzymes from the ligninolytic fungus phanerochaete flavido-alba. | phanerochaete flavido-alba is able to decolorize and detoxify olive oil wastewater (omw) in a process in which simple and polymeric phenols are removed. an unusual acidic mnp is accumulated during the degradation course. this microorganism produces two families of mnps. mnp1 has an apparent molecular weight of 45 kda and is secreted as a mixture of isoenzymes with pi ranging from 5.6 to 4.75. mnp2, which is produced as an unique isoenzyme, has an apparent molecular weight of 55.6 mr and an unusu ... | 2002 | 12437216 |
| phanerochaete chrysosporium nadph-cytochrome p450 reductase kinetic mechanism. | the recently completed genome of the basidiomycete, phanerochaete chrysosporium, revealed the presence of one nadph-cytochrome p450 oxidoreductase (cpr; ec 1.6.2.4) gene and >123 cytochrome p450 (cyp) genes. how a single cpr can drive many cyps is an important area of study. we have investigated this cpr to gain insight into the mechanistic and structural biodiversity of the cytochrome p450 catalytic system. native cpr and a nh(2)-terminally truncated derivative lacking 23 amino acids have been ... | 2002 | 12437968 |
| disseminated pulmonary adiaspiromycosis caused by emmonsia crescens in a horse. | | 2002 | 12462193 |
| polycyclic aromatic hydrocarbon removal from soil by surfactant solubilization and phanerochaete chrysosporium oxidation. | surfactant soil washing can remove polycyclic aromatic hydrocarbons (pahs) from contaminated soil, and the white rot fungus, phanerochaete chrysosporium burdsall in burdsall & eslyn, can oxidize pahs. the objective of this study was to develop a novel bioremediation technology using a combination of abiological surfactant soil washing followed by pah biological oxidation in soil washwater using p. chrysosporium in a rotating biological contactor (rbc) reactor. soil used for experimentation was a ... | 2002 | 12469833 |
| isolation of keratinophilic fungi from elementary schools and public parks in isfahan, iran. | a total of 330 dust and soil samples collected from different sites at 13 elementary schools and seven public parks in the province of isfahan, iran were examined for the frequency of keratinophilic fungi by the hair-baiting technique. two hundred and fourteen isolates of keratinophilic fungi belonging to seven species were identified. the most frequent isolate was chrysosporium keratinophilum (54.2%). the frequency and distribution of these keratinophilic fungi are discussed in relation to the ... | 2002 | 12472728 |
| mechanism of the reductive half-reaction in cellobiose dehydrogenase. | the extracellular flavocytochrome cellobiose dehydrogenase (cdh; ec ) participates in lignocellulose degradation by white-rot fungi with a proposed role in the early events of wood degradation. the complete hemoflavoenzyme consists of a catalytically active dehydrogenase fragment (dh(cdh)) connected to a b-type cytochrome domain via a linker peptide. in the reductive half-reaction, dh(cdh) catalyzes the oxidation of cellobiose to yield cellobiono-1,5-lactone. the active site of dh(cdh) is struct ... | 2003 | 12493734 |
| isolation and structure elucidation of two new antibacterial compounds produced by chrysosporium queenslandicum. | | 2002 | 12523825 |
| effect of culture conditions on manganese peroxidase production and activity by some white rot fungi. | the ligninolytic system of white rot fungi is primarily composed of lignin peroxidase, manganese peroxidase (mnp) and laccase. the present work was carried out to determine the best culture conditions for production of mnp and its activity in the relatively little-explored cultures of dichomitus squalens, irpex flavus and polyporus sanguineus, as compared with conditions for phanerochaete chrysosporium and coriolus versicolor. studies on enzyme production under different nutritional conditions r ... | 2003 | 12545383 |
| tandem cytochrome p450 monooxygenase genes and splice variants in the white rot fungus phanerochaete chrysosporium: cloning, sequence analysis, and regulation of differential expression. | phanerochaete chrysosporium, the model white rot fungus, uses p450 monooxygenation for oxidation of xenobiotic chemicals. to characterize its p450 systems, we have isolated two tandemly linked full-length p450 monooxygenase genes, pc-1 (2510bp) and pc-2 (2503bp). cdna sequence and splice site analysis showed multiple small putative introns (35-82bp), 17 in pc-1 and 16 in pc-2. the deduced p450 proteins pc-1 (531 aa, mw 60,070) and pc-2 (538 aa, mw 61,765) showed 55% aa identity with each other a ... | 2003 | 12553932 |
| [studies on biosorption of pb2+ by phanerochaete chrysosporium]. | the biosorption of pb2+ from solution by phanerochaete chrysosporium was studied. the effects on biosorption capacity of ph, pb2+ concentration in solution, co-ion and temperature, time have been examined. the adsorption capacity was compared among the biomass harvested at various growth stages. pretreatment of biomass by naoh solution improved its adsorption capacity. the uptake could reach 108.4 mg/g at optimal conditions of biosorption using pretreated biomass. | 1999 | 12555409 |
| [comparison of lignocellulolytic enzyme profiles secreted by panus conchatus and phanerochaete chrysosporium during solid state cultures]. | most of white-rot fungi, such as phanerochaete chrysosporium, can cause severe concomitant cellulose degradation during biodegradation of lignocellulose. panus conchatus, a white-rot fungus, can cause efficient delignification of straw with only limited concomitant cellulose degradation. the results in comparison of lignocellulolytic enzyme profiles secreted by p. conchatus and p. chrysosporium during solid state cultures have shown that laccase and mn-dependent peroxidase are main lignin-degrad ... | 1999 | 12555416 |
| [bioremediation for petroleum-contaminated soil by composting technology]. | with composting technology of off-site bioremediation, the bioremediation of soil contaminated by crude oil from liaohe oil field was studied. 4 treatments units were set, each units being 118.5 cm in length, 65.5 cm in width, and 12.5 cm in height. the results showed that when the soil was contaminated with 5.22 g.100 g-1 tph, the degradation rate of tph reached 54.2% after 55 days operation by phanerochaete chrysosporium. the results also indicated that the major factors that effected the tph ... | 2002 | 12561178 |
| dibenzyl sulfide metabolism by white rot fungi. | microbial metabolism of organosulfur compounds is of interest in the petroleum industry for in-field viscosity reduction and desulfurization. here, dibenzyl sulfide (dbs) metabolism in white rot fungi was studied. trametes trogii uamh 8156, trametes hirsuta uamh 8165, phanerochaete chrysosporium atcc 24725, trametes versicolor ifo 30340 (formerly coriolus sp.), and tyromyces palustris ifo 30339 all oxidized dbs to dibenzyl sulfoxide prior to oxidation to dibenzyl sulfone. the cytochrome p-450 in ... | 2003 | 12571066 |
| stimulation of ligninolytic enzyme production in phanerochaete chrysosporium by polyoxyalkanes. | poly(ethylene glycol) (peg) and some substances similar to peg in chemical structure were tested as stimulators of ligninolytic enzyme production in shaken culture of phanerochaete chrysosporium. | 2003 | 12588543 |
| study of the degradation of dyes by mnp of phanerochaete chrysosporium produced in a fixed-bed bioreactor. | the production of ligninolytic enzymes by the fungus phanerochaete chrysosporium in a fixed-bed tubular bioreactor, filled with cubes of nylon sponge, operating in semi-solid-state conditions, was studied. maximum individual manganese-dependent peroxidase (mnp) and lignin peroxidase (lip) activities of 1293 and 225 u/l were detected. the in vitro decolourisation of two structurally different dyes (poly r-478, crystal violet) by the extracellular liquid obtained in the above-mentioned bioreactor ... | 2003 | 12604081 |
| cleavage of nonphenolic beta-1 diarylpropane lignin model dimers by manganese peroxidase from phanerochaete chrysosporium. | purified manganese peroxidase (mnp) from phanerochaete chrysosporium oxidizes nonphenolic beta-1 diarylpropane lignin model compounds in the presence of tween 80, and in three- to fourfold lower yield in its absence. in the presence of tween 80, 1-(3',4'-diethoxyphenyl)-1-hydroxy-2-(4'-methoxyphenyl)propane (i) was oxidized to 3,4-diethoxybenzaldehyde (ii), 4-methoxyacetophenone (iii) and 1-(3',4'-diethoxyphenyl)-1-oxo-2-(4'-methoxyphenyl)propane (iv), while only 3,4-diethoxybenzaldehyde (ii) an ... | 2003 | 12605679 |
| production and characterization of recombinant phanerochaete chrysosporium beta-glucosidase in the methylotrophic yeast pichia pastoris. | the extracellular beta-glucosidase from the white-rot fungus phanerochaete chrysosporium was expressed heterologously in the methylotrophic yeast pichia pastoris. after 7 days' cultivation in an induction medium containing 1% (v/v) methanol, the expression level of the recombinant enzyme was 28,500 u/l, 38 times that of the wild-type enzyme. the specific activity of the crude recombinant enzyme for p-nitrophenyl-beta-d-glucoside was 52 u/mg, 37 times that of the wild-type enzyme; this difference ... | 2003 | 12619666 |
| seasonal effects on the aerobic bacterial and fungal conjunctival flora of normal thoroughbred brood mares in florida. | to evaluate seasonal effects on the presence or absence of fungal and aerobic bacterial flora of the conjunctival fornix of normal florida thoroughbred horses. | 2003 | 12641842 |
| the catalytic module of cel7d from phanerochaete chrysosporium as a chiral selector: structural studies of its complex with the beta blocker (r)-propranolol. | previous investigations have shown that the major cellobiohydrolase of phanerochaete chrysosporium, cel7d (cbh 58), can be used to separate the enantiomers of a number of drugs, including adrenergic beta blockers such as propranolol. the structural basis of this enantioselectivity is explored here. a 1.5 a x-ray structure of the catalytic domain of cel7d in complex with (r)-propranolol showed the ligand bound at the active site in glucosyl-binding subsites -1/+1. the catalytic residue glu207 mak ... | 2003 | 12657782 |
| role of the flavin domain residues, his689 and asn732, in the catalytic mechanism of cellobiose dehydrogenase from phanerochaete chrysosporium. | cellobiose dehydrogenase is an extracellular flavocytochrome, which catalyzes the oxidation of cellobiose and other soluble oligosaccharides to their respective lactones, while reducing various one- and two-electron acceptors. two residues at the active site of the flavin domain, his689 and asn732, have been proposed to play critical roles in the oxidation of the substrate. to test these proposals, each residue was substituted with either a gln, asn, glu, asp, val, ala, and/or a his residue by s ... | 2003 | 12680758 |