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phenotypic classes of phenoloxidase-negative mutants of the lignin-degrading fungus phanerochaete chrysosporium.this paper reports the isolation of phenoloxidase-negative mutants of the white-rot fungus phanerochaete chrysosporium and the results of a survey of idiophasic functions among these mutants. the mutant strains were isolated from a medium containing o-anisidine after gamma irradiation of wild-type spores and fell into four classes, divided by the manner in which they mineralized 14c-lignin wheat lignocellulose. examples are strain lmt7, which degraded lignin at a rate similar to that of the wild ...19853921527
oxidation of persistent environmental pollutants by a white rot fungus.the white rot fungus phanerochaete chrysosporium degraded ddt [1,1,-bis(4-chlorophenyl)-2,2,2-trichloroethane], 3,4,3',4'-tetrachlorobiphenyl, 2,4,5,2',-4',5'-hexachlorobiphenyl, 2,3,7,8-tetrachlorodibenzo-p-dioxin, lindane (1,2,3,4,5,6-hexachlorocylohexane), and benzo[a]pyrene to carbon dioxide. model studies, based on the use of ddt, suggest that the ability of phanerochaete chrysosporium to metabolize these compounds is dependent on the extracellular lignin-degrading enzyme system of this fun ...19853925550
a cellulase assay coupled to cellobiose dehydrogenase.an assay for cellulase activity based on the oxidation of cellobiose, formed during the cellulase reaction, with ferricyanide and a cellobiose dehydrogenase derived from the cellulolytic fungus sporotrichum (chrysosporium) thermophile is presented. due to the restricted specificity of this enzyme for cellobiose and cellodextrins, glucose, which may be formed by the action of some cellulolytic components or by beta-glucosidase, does not contribute to the result. the negative interference of beta- ...19853925812
[the lignolytic activity of some white-rot fungi of the genus pleurotus].in this study, phenoloxidase activities of some fungi, pleurotus sajorcaju, p. sapidus, p. astreatus and phanerochaete chrysosporium which are usually considered in white - rot fungal group, were measured. the phenoloxidase activities of all the species in pleurotus genus were found to be higher than that of p. chrysosporium. when the lignolytic activities of p. sajor - caju and p. chrysosporium were compared, p. sajor - caju was found to have higher activity than that of p. chrysosporium.19853929035
adiaspiromycosis in striped skunks in alberta, canada.pulmonary adiaspiromycosis was diagnosed in seven of 25 striped skunks (mephitis mephitis) in east-central alberta. the infection varied from mild, where only microscopic lesions were seen, to severe, where gross lesions of grayish-white nodules were observed in the lung parenchyma. mild lesions were restricted to the lung, while severe lesions extended to the tracheobronchial and mediastinal lymph nodes. histologically, the lesions were characterized by a centrally located fungal spherule, surr ...19863951052
reliability of exoantigens for differentiating blastomyces dermatitidis and histoplasma capsulatum from chrysosporium and geomyces species.a recent study suggested that chrysosporium species have the same diagnostic antigens as histoplasma capsulatum and blastomyces dermatitidis and, thus, compromise the antigenic identification of these pathogens. in light of these findings, studies were undertaken to determine the reliability of the exoantigen tests for identifying b. dermatitidis and h. capsulatum organisms from cultures. sixty-three slant or shake culture extracts, or both, were derived from c. asperatum, c. keratinophilum, c. ...19863956141
purification and characterization of glucose oxidase from ligninolytic cultures of phanerochaete chrysosporium.glucose oxidase, an important source of hydrogen peroxide in lignin-degrading cultures of phanerochaete chrysosporium, was purified to electrophoretic homogeneity by a combination of ion-exchange and molecular sieve chromatography. the enzyme is a flavoprotein with an apparent native molecular weight of 180,000 and a denatured molecular weight of 80,000. this enzyme does not appear to be a glycoprotein. it gives optimal activity with d-glucose, which is stoichiometrically oxidized to d-gluconate ...19863957868
[1st cases of adiaspiromycosis observed in small mammals in turkey. 3 new host species].the authors examine the lungs of 193 small mammals caught in different places in west turkey and belonging to 11 different species. six species have one or many adiaspores in their lungs. those are microtus arvalis, apodemus flavicollis and a. sylvaticus already found parasited by chrysosporium parvum in many countries particularly france. these are m. guentheri, pitymys majori and a. mystacinus which have never been mentioned before this day having adiaspiromycosis.19853986950
multiple molecular forms of diarylpropane oxygenase, an h2o2-requiring, lignin-degrading enzyme from phanerochaete chrysosporium.three different molecular forms of the h2o2-requiring heme enzyme, diarylpropane oxygenase, were isolated from the extracellular medium of na-acetate buffered, agitated cultures of phanerochaete chrysosporium. forms i, ii, and iii were separated by deae-sepharose and further purified on sephadex g-100. absorption maxima of the native, reduced, and a variety of ligand complexes of the three enzyme forms are essentially identical, indicating similar heme environments. all forms also have similar, ...19854026322
lack of lignin degradation by glucose oxidase-negative mutants of phanerochaete chrysosporium.glucose oxidase-negative (gox-) mutants of phanerochaete chrysosporium were isolated after exposing conidia to uv irradiation. the gox- mutants exhibited little or no ability to degrade lignin (2-[14c]-synthetic lignin to 14co2); however, they retained other secondary metabolic features such as the ability to conidiate and produce veratryl alcohol, suggesting that they are not pleiotropic for secondary metabolism. lignin degradation activity was restored in gox+ revertants. these results, in sup ...19854038305
a new human case of anixiopsis stercoraria mycosis: discussion of its taxonomy and pathogenicity. 19854058480
purification and characterization of an extracellular mn(ii)-dependent peroxidase from the lignin-degrading basidiomycete, phanerochaete chrysosporium.a mn(ii)-dependent peroxidase found in the extracellular medium of ligninolytic cultures of the white rot fungus, phanerochaete chrysosporium, was purified by deae-sepharose ion-exchange chromatography, blue agarose chromatography, and gel filtration on sephadex g-100. sodium dodecyl sulfate-gel electrophoresis indicated that the homogeneous protein has an mr of 46,000. the absorption spectrum of the enzyme indicates the presence of a heme prosthetic group. the pyridine hemochrome absorption spe ...19854062285
[adiaspiromycosis of the lungs]. 19854095621
experimental adiaspiromycosis in rabbits: host response to chrysosporium parvum and c. parvum var. crescens antigens. 19744276215
[87. isolation of keratinophilic fungi from the soil using the hair-lure method. determination of microsporum gypseum (nannizia incurvata) and trichophyton terrestre from the austrian soil, keratinomyces ajelloi from the danish soil and chrysosporium keratinophilum from japanese soil]. 19724673848
chrysosporium parvum from eczematous lesions in man. 19734807409
[revision of thermophilic sporotrichum species: chrysosporium thermophilum (apinis) comb. nov. and chrysosporium fergusii spec. nov. equal status conidialis of corynascus thermophilus fergus and (sinden) comb. nov]. 19744858767
[chrysosporium indicum-like fungi and chrysosporium evolceanui from the soil of cuba]. 19684920269
metabolites from a chrysosporium species. 19715168359
[reproduction of emmonsia crescens in vitro and in vivo]. 19715212493
thermotolerance of chrysosporium tropicum differentiates it from c. keratinophilum. 19695379287
[a keratinophilic strain of chrysosporium with outspoken dermatophytic, immunobiologic properties from guano in a cave in rumania (imperfect stage of arthroderma multifidum-dawson 1963?) i]. 19675628748
emmonsia brasiliensis and emmonsia ciferrina are chrysosporium pruinosum. 19685689786
[dermatophyte flora of the soil of asia minor and rhodes. isolation of trichophyton quinckeanum, trichophyton terrestre, chrysosporium keratinophilum and chrysosporium inducum]. 19685761166
[the occurrence of chrysosporium keratinophilum (frey) carmichael and a thermophylic variant in soil and in animals]. 19685761204
extracellualr enzyme system utilized by the fungus chrysosporium lignorum for the breakdown of cellulose. i. studies on the enzyme production. 19695812970
extracellular enzyme system utilized by the fungus chrysosporium lignorum for the breakdown of cellulose. ii. separation and characterization of three cellulase peaks. 19695812971
parthenogenetic production of fertile cleistothecia in nannizzia incurvata stockd. under the influence of chrysosporium species. 19695815313
an addition to the genus chrysosporium corda. 19666008555
occurrence of keratinophilic chrysosporium corda species in indian soils. 19666009034
gelatin hydrolysis for identification of the filamentous phase of histoplasma, blastomyces and chrysosporium species. 19676069120
possible relationship between cyclic amp and idiophasic metabolism in the white rot fungus phanerochaete chrysosporium.in the hymenomycete phanerochaete chrysosporium, a 10-fold increase in intracellular cyclic amp preceded the onset of the two idiophasic activities, lignin degradation and veratryl alcohol production. further, addition of l-glutamate during this period reduced cyclic amp levels and suppressed ligninolytic activity.19846090435
comparative study of a new chrysosporium species with histoplasma capsulatum.the chrysosporium state of a new ascomycete, renispora flavissima (gymnoascaceae), resembles histoplasma capsulatum in its macroconidial morphology. it was discovered growing in bat guano, from which it was readily isolated by direct plating of diluted soil, but only rarely from mice inoculated with soil suspensions. the fungus was consistently reisolated from tissues of mice inoculated intravenously and intraperitoneally with conidial and mycelial suspensions from cultures of the fungus. nevert ...19806158762
keratinophilic fungi from orissa, india, i: isolation from soils.results of a preliminary survey of keratinophilic fungi in soils collected from five districts of orissa, india, are reported. a total of 80 soil samples were examined, of which 51 samples (63.7%) were positive for keratinophilic fungi. twelve species of fungi classified in seven genera were isolated: aphanoascus reticulisporus (2.4%), aph. terreus (1.2%), chrysosporium state of arthroderma tuberculatum (4.8%), chrysosporium indicum (31.3%), chrys. tropicum (18.0%), chrys. evolceanui (1.2%), gym ...19806161419
keratinophilic fungi from orissa, india, ii: isolations from feathers of wild birds and domestic fowls.feather samples from 117 birds, representing 11 species, were examined for keratinophilic fungi. of these, 63 (53.8%) were found to be positive. nine species of fungi classified in 6 genera were isolated: aphanoascus terreus (1.7%), chrysosporium indicum (26.4%), chrys. tropicum (11.1%) the chrysosporium state of aphanoascus spp. (2.5%), the chrysosporium state of arthroderma tuberculatum (3.4%), ctenomyces serratus (0.8%), gymnoascoideus petalosporus (7.6%), malbranchea spp. (6.8%), rollandina ...19806161420
investigation of keratinophilic fungi from soils in western australia a preliminary survey.in order to determine which species of geophilic dermatophytes were present in western australian soils 299 samples were investigated. these samples were collected from a range of locations, 208 towns throughout the state and 91 samples from the perth metropolitan area. most samples were collected from areas frequented by people and animals, such as home gardens, parks and animal yards. of the total 299 soils, 271 (90.6%) yielded keratinophilic fungi. a total of 181 dermatophytes were isolated, ...19806162100
effect of treatment of soil with keratinaceous material on soil fungi.in the early periods of soil storage, the total number of fungi almost regularly and significantly increased with the concentration of keratinaceous material (ground buffalo hooves) between 1% and 10%. a concentration of 20% was stimulative after 7 days and became toxic after 15 days and remained so through the end of the experiment. with increase in the storage period, the beneficial effect of all concentrations was lost and in the case of high concentrations (5-20%) toxic ammonia was produced. ...19816171708
keratinolytic fungi in egyptian soils.the frequency of occurrence of keratinolytic fungi in seventy soil samples collected from different sites in upper egypt and in the coastal area of the mediterranean and baited with human and animal hair and pigeon feathers was determined. twenty-one species in addition to an unidentified species, which belong to sixteen genera were collected. chrysosporium indicum, b. tropicum, c. keratinophilum and microsporum gypseum were the most frequent fungal species recovered from baited soils. the eight ...19826181404
nutritional requirements of keratinophilic fungi and dermatophytes for conidial germination.germination of spores of chrysosporium crassitunicatum, nannizzia fulva (+), nannizzia fulva (-) and trichophyton equinum was studied in the presence of various carbon and nitrogen sources. effect of different temperatures on spore germination was also determined. maximum spore germination within 24 hours was recorded when glucose was used as a carbon source for all the test fungi. except sodium nitrate all the inorganic nitrogen sources enhanced the spore germination at 0.05% concentration. mos ...19826184619
isolation of keratinophilic fungi from floors in roman primary schools.keratinophilic fungi are present in the environment with variable distribution patterns that depend on different factors, one of which, of fundamental importance, is human and or animal presence. the present study was conducted in the environment and classrooms of schools in order to evaluate the relationship between the human presence and the presence of keratinophilic fungi. in order to achieve this goal, a new isolation technique was used. from 20 samples, 253 colonies of keratinophilic fungi ...19836193424
keratinophilic fungi isolated from the air at pavia.the presence of keratinophilic fungi was revealed by sampling the air of pavia (italy) from march 1981 to february 1982. the species isolated were: chrysosporium indicum , geomyces pannorum var. pannorum , microsporum gypseum, myceliophtora vellerea and trichophyton terrestre . several filamentous fungi tolerating high levels of cycloheximide were also recovered.19846203038
pulmonary adiaspiromycosis of some free living small mammals in the north moravian region: pathologic findings and experimental-biological observations. 19826211915
mutants of chrysosporium parvum var. crescens--a causative agent of adiaspiromycosis. 19836229148
pulmonary adiaspiromycosis of some free living small mammals in the north moravian region: pathologic findings and experimental-biological observations. 19826242567
the involvement of hydroxyl radical derived from hydrogen peroxide in lignin degradation by the white rot fungus phanerochaete chrysosporium.the possible involvement of hydrogen peroxide (h2o2)-derived hydroxyl radical (.oh) in lignin degradation ([14c]lignin leads to 14co2) by phanerochaete chrysosporium was investigated. when p. chrysosporium was grown in low nitrogen medium (2.4 mm n), an increase in the specific activity for h2o2 production in cell extracts was observed to coincide with the appearance of ligninolytic activity and both activities appeared after the culture entered stationary phase. the production of .oh in lignino ...19826288685
generation of hydroxyl radical and its involvement in lignin degradation by phanerochaete chrysosporium. 19826295392
comparative susceptibility of the agents of adiaspiromycosis to imidazole derivatives in vitro.clotrimazole, econazole, ketoconazole and miconazole inhibited the growth of emmonsia crescens and e. parva in vitro. clotrimazole and econazole gave particularly low minimal inhibitory concentrations against the mycelial phase of the fungi, and minimal fungicidal concentrations of all four antifungals were about 2 to 4 times higher than the minimal inhibitory concentration. the adiasporic developmental phases of e. crescens and e. parva were slightly more sensitive to all the imidazole antifung ...19836314565
electrogenic symport of glucose and protons in membrane vesicles of phanerochaete chrysosporium.the white rot fungus, phanerochaete chrysosporium, is one of the few organisms with documented ability to degrade lignin. protoplasts from p. chrysosporium were disrupted by osmotic shock and membrane vesicles were isolated from the cell debris. the vesicles exhibit active glucose transport that is consistent with a glucose/h+ symport mechanism. an artificial gradient of h+ (outside greater than inside) stimulates glucose uptake. conversely, a glucose gradient (outside greater than inside) resul ...19846320738
dna sequences from a ligninolytic filamentous fungus phanerochaete chrysosporium capable of autonomous replication in yeast.five different dna sequences of phanerochaete chrysosporium capable of supporting autonomous replication of yeast integration plasmid (yip5) in saccharomyces cerevisiae were isolated. these hybrid plasmids with the autonomous replication sequences from p. chrysosporium are maintained extra-chromosomally, are mitotically unstable and transform ura3 deletion mutant of s. cerevisiae to ura+ phenotype with high frequency. the autonomous replication sequence in prr2, one of the recombinant plasmids, ...19846322767
evidence for the involvement of activated oxygen in fungal degradation of lignocellulose.oxygen has been shown to be necessary as a cosubstrate for the fungal degradation of lignins. in this work, the active forms of oxygen were tentatively identified in three ways: --effect of chemically generated active radicals and molecular species on lignocellulosic complexes, --use of activated oxygen scavengers in culture media of ligninolytic fungi, --characterization of active forms of oxygen by specific reactions. the data obtained strongly suggest that two main oxygen species are involved ...19836409163
purification and characterization of an extracellular h2o2-requiring diarylpropane oxygenase from the white rot basidiomycete, phanerochaete chrysosporium.an h2o2-requiring oxygenase found in the extracellular medium of ligninolytic cultures of the white rot fungus phanerochaete chrysosporium was purified by deae-sepharose ion-exchange chromatography and gel filtration on sephadex g-100. sodium dodecyl sulfate (sds)-disc gel electrophoresis indicated that the purified protein was homogeneous. the mr of the enzyme as determined by gel filtration and sds-polyacrylamide gel electrophoresis was 41,000. the absorption spectrum of the enzyme indicated t ...19846497376
fatty acyl-coenzyme a oxidase activity and h2o2 production in phanerochaete chrysosporium mycelia.mycelia of the lignin-degrading fungus phanerochaete chrysosporium consume o2 and produce extracellular h2o2 when incubated with fatty acyl-coa substrates, even in the presence of mitochondrial respiratory chain inhibitors such as antimycin a and cyanide. these results suggest the possibility that peroxisomal fatty acyl-coa oxidase activity in p. chrysosporium mycelia may be an important metabolic source for the extracellular h2o2 believed to be involved in lignin biodegradation.19846538411
influence of animal hibernation on the development of mycoses.the development of adiaspiromycosis and trichophytosis depending upon the state of activity of red-cheeked squirrels is described. the conidia of chrysosporium (emmonsia) parvum var. crescens, are not transformed into adiaspores when injected into hibernating animals. during the hibernation period of four months, most of the conidia die. after awakening, the remaining viable conidia are transformed into adiaspores. during hibernation the squirrels, which had been infected with trichophyton menta ...19846538934
[adiaspiromycosis in small wild mammals caught in tunisia].the authors examine the lungs of 125 small mammals caught in different provinces of tunisia and belonging to 12 species; 4 apodemus sylvaticus, 1 mus spretus and 1 crocidura russula possess one adiaspore of emmonsia crescens = chrysosporium parvum or more hundred. they find adiaspiromycosis only in damp biotopes.19846541531
chryscandin, a novel peptidyl nucleoside antibiotic. i. taxonomy, fermentation, isolation and characterization.a novel antifungal antibiotic, chryscandin was found in the culture broth of a strain of fungi. the producing organism was subsequently identified as chrysosporium pannorum. the antibiotic obtained as colorless needle crystals (c20h23n7o6, mw 457) is active against candida albicans and gram-positive bacteria, but it is ineffective against filamentous fungi and gram-negative bacteria. acute toxicity is very low in mice.19846549000
chryscandin, a novel peptidyl nucleoside antibiotic. ii. structure determination and synthesis.the structure of chryscandin, a novel antifungal antibiotic, produced by chrysosporium pannorum no. 4629 was deduced to be 1 from spectroscopic and chemical evidences. in order to confirm the structure and to determine the absolute configuration, the total synthesis of chryscandin was performed. d-xylose was transformed into 10 in ten steps. after beta-glycosidation of silylated benzoyl adenine with 10, the resulting 11 was converted into 4, which was identical with the product obtained from chr ...19846549001
activity of isocitrate dehydrogenase from three filamentous fungi in relation to osmotic and solute effects.crude extracts of nadp+-specific isocitrate dehydrogenase (ec 1.1.1.42) were prepared from three filamentous fungi with different tolerances to water stress. there was no difference in the activity of this enzyme extracted from phytophthora cinnamomi which had been grown on media of osmotic potential of 0 to -2mpa. glycerol, proline and glucose caused little or no inhibition of the activity of the enzyme from p. cinnamomi, penicillium chrysogenum and chrysosporium fastidium over the range 0 to - ...19836615128
an extracellular h2o2-requiring enzyme preparation involved in lignin biodegradation by the white rot basidiomycete phanerochaete chrysosporium.an h2o2-requiring enzyme system was found in the extracellular medium of ligninolytic cultures of phanerochaete chrysosporium. the enzyme system generated ethylene from 2-keto-4-thiomethyl butyric acid (ktba), and oxidized a variety of lignin model compounds including the diarylpropane 1-(4'-ethoxy-3'-methoxyphenyl) 1,3-dihydroxy-2-(4"-methoxyphenyl)propane (i), a beta-ether dimer 1-(4'-ethoxy-3'-methoxyphenyl)glycerol-beta-guaiacyl ether (iv) and an olefin 1-(4'-ethoxy-3'-methoxyphenyl)1,2-prop ...19836615503
human pulmonary granulomas caused by chrysosporium parvum var. crescens (emmonsia crescens).multiple lung nodules were found at autopsy of a 56-year-old man who died of unrelated cause. the nodules contained adiaspores which were identified as those of chrysosporium parvum var. crescens.19836625072
substrate-induced h2o2 production in mycelia from the lignin-degrading fungus phanerochaete chrysosporium.broken and subsequently washed mycelia from the lignin-degrading fungus phanerochaete chrysosporium generated increased levels of h2o2 when exposed to a lignocellulosic substrate (ground wheat straw) or substrate analogue (poly b-411). h2o2 production was observed only in cultures grown on limiting nitrogen, a condition known to induce the lignin-degrading activity of this organism. h2o2 production was observed in the ph range of 4.5 to 8.4, but the highest levels of h2o2 were generated around p ...19836661224
fine structural studies of the chrysosporium state of renispora flavissima.aspects of the fine structural characteristics of conidial and hyphal cells of the imperfect chrysosporium state of the newly reported gymnoascaceous fungus, renispora flavissima, are described and illustrated by electron micrographs of ultrathin sections. preservation of the substructure of the thick-walled, smooth to tuberculate conidia was best achieved after fixation in permanganate. the conidial wall consisted of two conspicuous zones (or layers) of varying thickness, texture, and electron ...19836682180
isolation of keratinophilic fungi from sewage sludge.five mixed samples of sewage sludges from 4 wastewater treatment plants were examined for keratinophilic fungi. microsporum gypseum, trichophyton terrestre, t. ajelloi, chrysosporium pannorum, c. keratinophilum and c. pruinosum were recovered.19836685348
chrysosporium, a new causative agent in osteomyelitis. a case report.a 24-year-old black man presented with a two-month history of swelling of the right ankle. roentgenograms were initially read as negative. one month later the patient returned with increased pain and swelling, and roentgenograms demonstrated a large lucency involving the distal tibia. bone scanning revealed extension of the lesion proximally, to 6 cm below the knee. biopsy of a large tibial abscess demonstrated histocytic giant cells with budding yeasts and septate mycelia. chrysosporium was iso ...19846705347
temperature and water activity minima for growth of spoilage moulds from meat.five species of fungi were isolated from mould spoilage on meat other than black spot. 'white spot' colonies yielded chrysosporium pannorum or an acremonium sp.; 'whiskers' colonies yielded thamnidium elegans or mucor racemosus, and blue-green colonies yielded penicillium corylophilum . chrysosporium pannorum was moderately xerotolerant with a minimum growth temperature of -5 degrees c. the acremonium sp. and p. corylophilum showed a similar level of xerotolerance but had a minimum growth temper ...19846725157
effect of soil fungistasis on zoopathogenic fungi.the inhibiting action of west-siberian soils on spore germination and the growth and development of zoopathogenic fungi such as emmonsia (chrysosporium) crescens , trichophyton mentagrophytes, beauveria bassiana , metarrhizium anisopliae , paecilomyces farinosus , p. fumoso -roseus and chrysosporium keratinophilum have been studied by the authors. the influence of carbon sources and the root exudates of plants on fungistasis have also been studied.19846727979
[adiaspiromycosis--a little known lung disease].adiaspiromycosis produced by the fungus emmonsia crescens is a pulmonary disease afflicting primarily small wild mammals. man, too, may become an accidental link of the saproparatrophic circulation of the agent. humans are infected--similarly to other mammals--by inhaling the elements of the saprophytic stage of the fungus living for long time periods in the soil substrates. after the infecting cells, aleuriospores, have invaded the host lungs, they are converted into the elements of the parasit ...19826762779
[adiaspiromycosis in small mammals in large capacity pigsties in the trebon basin].from 1977 to 1979 a study on the occurrence of adiaspiromycosis was performed on three animal farms in south bohemia. in 113 examined small mammals from the farms and their neighbourhoods adiaspiromycosis was detected in m. arvalis living both in the fields in the precincts (7/613, i. e. 1.14%) and in the grassy areas inside the three animal farms (5/221, i. e. 2.3%). by that time no adiaspiromycosis was found in small mammals living in the farm buildings. the obtained results were compared with ...19816787784
further study discounts role for singlet oxygen in fungal degradation of lignin model compounds.this study reexamined our contention that singlet oxygen (1o2) plays a role in the fungal degradation of lignin (bbrc 102(1981)484). cultures of phanerochaete chrysosporium and a photochemical 1o2-generating system (riboflavin/light/o2) cleaved a lignin substructure model compound, 1,2-bis(4-methoxyphenyl)propane-1,3-diol (i), by indistinguishable mechanisms. however, the rate of cleavage of i in d2o was the same as in h2o in the photochemical 1o2-generating system, indicating that 1o2 was not i ...19836830588
investigation of white-rot fungi for the conversion of poplar into a potential feedstuff for ruminants.five isolates of wood-rotting fungi were compared for their ability to increase the digestibility of poplar shavings. homogenates of the fungi were inoculated into mixtures of 25 g of shavings and 100 ml of supplementary nutrient solution and incubated at 25 degrees c. the in vitro rumen digestibility of the products increased from 30% at the start of the fermentation to maxima of 72% with polyporus anceps after 8 weeks, 64% with ganoderma applanatum and 62% with phanerochaete chrysosporium afte ...19836850424
antifungal activity of pyrithione against emmonsia crescens in vitro and in vivo.the sensitivity of saprophytic and parasitic phase of some strains of emmonsia crescens, e. parva and chrysosporium pannorum to 22 antimycotic drugs was studied in vitro. pyrithione produced the best effect on both live phases of the studied strains. the efficacy of pyrithione, pimafucin and amphotericin b was compared in experimental adiaspiromycosis, pyrithione showed also in vivo the best therapeutic effect. it depended on the beginning of the application. the illness did not develop if pyrit ...19826890013
disseminated pulmonary adiaspiromycosis identified in bioptic material from the lung.a disseminated form of pulmonary adiaspiromycosis was estimated in a 48 year old miner. the origin of a hypersensitivity mainly of an immediate allergic type became evident during an immunological examination and was partly indicated also by the tissue reaction and by elevated level of serum ige (480 i.u.). of great interest was the finding of a coagulation necrosis in the bioptic material. an examination of functions disclosed signs of a moderate obstruction which was latent when first observed ...19826892182
[history of the discovery and study of the causative agent of adiaspiromycosis]. 19807007832
survey of human pathogenic actinomycetes and fungi in soil from rome and other italian areas.as part of a study sponsored by the ministry of health of italy, a research program on pathogenic actinomycetes, keratinophilic and pathogenic fungi in soil was carried out. two hundred soil samples, collected from different areas of the city of rome, calabria, emilia romagna, latium, apulia, sardinia, sicily, tuscany and umbria, were examined by several techniques to detect the widest possible variety of pathogenic actinomycetes and fungi. seven isolates of nocardia asteroides, four of actinoma ...19817012635
characterization of a cellobiose dehydrogenase in the cellulolytic fungus sporotrichum (chrysosporium) thermophile.an extracellular enzyme from culture filtrates of sporotrichum (chrysosporium) thermophile (a.t.c.c. 42 464) after growth on cellulose or cellobiose was shown to oxidize cellobiose to cellobionic acid in vitro. lactose and cellodextrins were also efficiently oxidized, but the enzyme was not active against most mono- and di-saccharides. several redox substances could act as electron acceptors, but molecular oxygen, tetrazolium salts and nad(p) were not reduced. activity was stimulated up to 2-fol ...19827103940
keratinolytic fungi in egyptian soils.the frequency of occurrence of keratinolytic fungi in seventy soil samples, collected from different sites in upper egypt and in the coastal area of the mediterranean, was determined by baiting with human and animal hairs and pigeon feathers. twenty-one species, in addition to an unidentified species, which belong to sixteen genera were collected. chrysosporium indicum, c. tropicum, c. keratinophilum, and microsporum gypseum were the most frequent fungal species recovered from the baited soils. ...19827109977
[contribution to active and passive immunisation in deep-seated mycoses. chrysosporium parvum var. crescens as a model]. 19827121512
ethylene production from alpha-oxo-gamma-methylthiobutyric acid is a sensitive measure of ligninolytic activity by phanerochaete chrysosporium.production of hydroxyl radical in ligninolytic cultures was determined by measuring the alpha-oxo-gamma-methylthiobutyric acid-dependent production of ethylene gas. the results showed that the pattern of ethylene production was very similar to that of ligninolytic activity [[14c]lignin leads to 14co2). furthermore, nutritional parameters, which are known to affect ligninolytic activity, affected oh.-radical-dependent ethylene production in a similar fashion. the results indicate that assay for e ...19827150253
[mycological diagnosis and study of mycetes isolated from an area along the tyrrhenian coast in the province of catanzaro (calabria)].10 areas along the tyrrhenian coast were studied: 37 genera of hyphomycetes were isolated from 100 sand samples. the genera aspergillus, fusarium, alternaria, penicillium, dendryphiopsis, were constantly present. many of the fungi isolated occurred only rarely and were represented by trichoderma, sepedonium, cladosporium and chrysosporium. the genus geotrichum was the most prevalent of the unpigmented yeasts which were predominant (59.6%), while rhodotorula was the main genus in pink yeasts (40. ...19827167635
[presence of adiaspores of emmonsia crescens emmons and jellison, 1960, a moniliaceous, aleurioid fungus, in the lungs of small wild mammals in northern morocco].the authors examine the lungs of 133 small mammals caught in the north of morocco and belonging to 7 species: 12,8% of the animals (14/95 a. sylvaticus, 1/18 m. spretus and 2/11 c. russula) possess one adiaspore of e. crescens or more hundred. the relatively damp climate of this region can explain this strong percentage of infestation. that is the first time that the adiaspiromycosis is noticed in small mammals in north africa and the second time on the african continent.19807191355
[peritoneal form of adiaspiromycosis (author's transl)].a female patient, aged 30 years was subjected in 1977 to an appendectomy because of a perforated gangrenous appendix associated with purulent peritonitis. postoperatively she developed an intractable constipation persisting for more than one year. because of suspected intestinal stenosis an exploratory laparotomy was performed which failed to disclose the presence of stenosis. histological studies of excised peritoneal specimens were suggestive of tuberculous peritonitis. since cultivation faile ...19807194542
the microbiology of spent mushroom compost and its dust.microorganisms in spent steamed mushroom compost and its dust were enumerated, and identified. some phase ii (indoor composting) compost samples were also examined. steaming of spent compost resulted in a 70-76% reduction in microbial numbers. total counts made with compost fusion agar were approximately two logs greater than those for nutrient agar. the most common bacterial isolate was bacillus licheniformis. the most common actinomycete isolates were streptomyces diastaticus and thermoactinom ...19817197578
[gilchrist's disease and fungal taxonomy: a thorny problem].the study of iconographical data and of six american and african strains of b. dermatitidis allows us to conclude that there are several discriminating characters between the members of the blastomyces dermatitidis complex. the african strains are distinguished by the lack of sexual forms and old strains show the lost of the m lead to y conversion. an identical character prevails among the strains of the two continents: tuberculate conidia which are obtained on corn meal agar. the authors are in ...19817197591
adiaspiromycosis in south australian hairy-nosed wombats (lasiorhinus latifrons).spherical organisms, with an average diameter of about 22 microns, were detected in the lungs of adult and pouched young hairy-nosed wombats (lasiorhinus latifrons). although infections of up to 640 x 10(3) organisms per cubic centimeter were detected, their presence produced only limited pathological change. in-vitro growth was obtained at 30 c but not at 37 c or 40 c. however, at the higher temperatures, typical chlamydospore spherules were produced by colonies initially grown at 30 c. this re ...19827201528
effects of molecular oxygen on lignin degradation by phanerochaete chrysosporium. 19817236274
[molds in foods of the czechoslovak socialistic republik (author's transl)].the mycoflora of 13.527 g of food powders which were normal upon sensory evaluation as well of 22.017 pieces of surface sterilized grains and nuts was examined in the czechoslovak socialist republic. 147.027 colonies of 44 genera of molds were isolated and identified from samples. colonies of penicllium sp., aspergillus sp., and cladosporium sp. occured most often, less often or quite infrequently rhizopus sp., mucor sp., absidia sp., alternaria sp., circinella sp., trichoderma sp., botrytis sp. ...19817269803
adiaspiromycosis in tasmanian wombats?the finding of small spherules in the lungs of two species of wombats from tasmania is described. an histological examination of lung tissue caused adiaspiromycosis to be suspected and the etiological agent was thought to chrysosporium parvum.19807393314
isolation of dermatophytes and correlated species from the soil of public gardens and parks in rome.looking for dermatophytes and other keratinophilic fungi in the soil of parks and gardens in large towns is very interesting because of the relationship between the number of people living in the area and the presence of such fungi in the soil. as compared with other cities in italy, rome offers prime conditions for this kind of research. it has a high population density (10,000 inhabitants per sq/km2), and many parks and gardens, where it is possible to carry out soil studies. it was noted that ...19807423331
lignin degradation by phanerochaete chrysosporium in hyperbaric oxygen.phanerochaete chrysosporium degraded aspen wood lignin as well in 2 atm o2 (1 atm = 101.325 kpa) as in 1 atm o2, but 3 atm o2 inhibited the fungus, and o2 pressures above 4 atm killed it. lignin degradation in 5 atm of air was similar to that in 1 atm of o2, indicating that o2 concentration, not pressure, was the inhibitory factor. the selectivity with which p. chrysosporium metabolised lignin in preference to other wood components did not increase at o2 pressures above 1 atm.19807459728
isolation of dermatophytes and other keratinophilic fungi from surface sediments of the shatt al-arab river and its creeks at basrah, iraq.twenty-five sediment samples were taken from randomly selected sites in the shatt al-arab river and its creeks and analysed for dermatophytes and related keratinophilic fungi. the results revealed that out of 25 samples only 13 (52%) yielded dermatophytes and related keratinophilic fungi. a total of nine species in four genera were isolated. the most frequent genera isolated in this study were chrysosporium and its teleomorph aphanoascus. the species most frequently found were aphanoascus fulves ...19957477095
radical intermediates during degradation of lignin-model compounds and environmental pollutants: an electron spin resonance study.1. i discuss the following aspects of phanerochaete chrysosporium ligninase, the enzyme that has been shown to degrade a number of lignin-model compounds, and environmentally significant polycyclic aromatic hydrocarbons and polychlorinated phenols. 2. the primary mode of oxidation involves formation of a cation radical, which undergoes either hydrolysis or carbon-carbon bond cleavage. 3. in the presence of reducing substrate such as oxalic acid, ligninase has reductive activity that has been sho ...19957483665
substrate-dependent differential splicing of introns in the regions encoding the cellulose binding domains of two exocellobiohydrolase i-like genes in phanerochaete chrysosporium.recently, we have shown differential splicing of an intron in the cbhi.2 gene of phanerochaete chrysosporium me446; this intron lies within the region of the gene encoding the cellulose binding domain (p.f.g. sims, m. s. soares-felipe, q. wang, m.e. gent, c. tempelaars, and p. broda, mol. microbiol. 12:209-216, 1994). here, we show that such differential splicing occurs in the cbhi.1 gene of this fungus as well as in the cbhi.2 gene and that this phenomenon is substrate dependent. avicel elicits ...19957487009
expression of fungal mn peroxidase in e. coli and refolding to yield active enzyme.the cdna encoding mn peroxidase isozyme h4 from phanerochaete chrysosporium was expressed in escherichia coli. the portion of the cdna encoding the enzyme's signal peptide, not found in the processed holoenzyme, was deleted from the cdna. the polypeptide was produced as inactive inclusion bodies that could be solubilized in 8 m urea and the reducing agent dithiothreitol. reconstitution of activity was accomplished by diluting the urea concentration to 2m in the presence of hemin, calcium, and ox ...19957488173
keratinolysis and its morphological expression in hair digestion by airborne fungi.the morphological expression of keratinolysis in fungi isolated from the air of torino (98 isolates belonging to 36 species) was studied. light microscopy on whole material and on semithin sections, as well as scanning electron microscopy was used. there were 19 keratinolytically active species, with seven in the genus chrysosporium (c. indicum, c. keratinophilum, c. pannicola, c. tropicum, c. an. arthroderma cuniculi, c. an. pectinotrichum llanense, c. an. renispora flavissima), four in the gen ...19947527126
screening of different keratin baits for isolation of keratinophilic fungi.twenty different keratin-rich substrates were screened as keratin baits for isolation of keratinophilic fungi from different soil sample of chhindwara district, india. fungi easily formed colonies on different baits. however, hair, feathers and horns were found to be more suitable than nails, fish fins and fish scales.19947537862
the effect of manganese on the oxidation of chemicals by lignin peroxidase.it has recently been discovered that lignin peroxidase isozyme h2 (liph2) has the ability to oxidize mn2+ (khindaria et al., 1995). furthermore, at ph 4.5, the physiological ph of phanerochaete chrysosporium, liph2 oxidizes mn2+ at a much faster rate (25 times) than veratryl alcohol (va). the ability of mn2+ to act as a redox mediator for indirect oxidations catalyzed by liph2 was therefore investigated. in the presence of physiologically relevant levels of oxalate and mn2+, the rate of liph2-ca ...19957548012
x-ray absorption spectroscopy comparison of the active site structures of phanerochaete chrysosporium lignin peroxidase isoenzymes h2, h3, h4, h5, h8, and h10.the iron heme and its immediate environment can provide information that is pivotal to our understanding of the structural and mechanistic features that confer unusual properties to the heme peroxidases. x-ray absorption spectroscopy (xas), which is ideally suited for the investigation of the local environment and electronic structure of the heme iron of hemeproteins, has been used to characterize a variety of lignin peroxidase and manganese-dependent peroxidase isoenzymes produced by the white ...19957548080
a report on the predominant occurrence of a dermatophyte species in cultivated soil from kuwait.from several soil samples screened for the occurrence of keratinolytic fungi, soils cultivated with ornamental plants were found to contain strains of the dermatophytic fungus microsporum gypseum. one soil sample was dominated by this species.19957566069
correlation of brightening with cumulative enzyme activity related to lignin biodegradation during biobleaching of kraft pulp by white rot fungi in the solid-state fermentation system.biobleaching of hardwood unbleached kraft pulp (ukp) by phanerochaete chrysosporium and trametes versicolor was studied in the solid-state fermentation system with different culture media. in this fermentation system with low-nitrogen and high-carbon culture medium, pulp brightness increased by 15 and 30 points after 5 days of treatment with t. versicolor and p. chrysosporium, respectively, and the pulp kappa number decreased with increasing brightness. a comparison of manganese peroxidase (mnp) ...19957574600
mineralization of mono- and dichlorobenzenes and simultaneous degradation of chloro- and methyl-substituted benzenes by the white rot fungus phanerochaete chrysosporium.phanerochaete chrysosporium extensively degraded and mineralized chlorobenzene and o-, m-, and p-dichlorobenzenes. the rate of degradation was in the following order: monochlorobenzene > m-dichlorobenzene > o-dichlorobenzene > p-dichlorobenzene. net level of degradation was generally higher than mineralization. maximal degradation and mineralization of chlorobenzenes were observed in malt extract cultures in which the lignin peroxidases and manganese peroxidases are not known to be produced. the ...19957574605
new polymeric model substrates for the study of microbial ligninolysis.lignin model dimers are valuable tools for the elucidation of microbial ligninolytic mechanisms, but their low molecular weight (mw) makes them susceptible to nonligninolytic intracellular metabolism. to address this problem, we prepared lignin models in which unlabeled and alpha-14c-labeled beta-o-4-linked dimers were covalently attached to 8,000-mw polyethylene glycol (peg) or to 45,000-mw polystyrene (ps). the water-soluble peg-linked model was mineralized extensively in liquid medium and in ...19957574649
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