| manganese regulates expression of manganese peroxidase by phanerochaete chrysosporium. | the appearance of manganese peroxidase (mnp) activity in nitrogen-limited cultures of phanerochaete chrysosporium is dependent on the presence of manganese. cultures grown in the absence of mn developed normally and produced normal levels of the secondary metabolite veratryl alcohol but produced no mnp activity. immunoblot analysis indicated that appearance of mnp protein in the extracellular medium was also dependent on the presence of mn. intracellular mnp protein was detectable only in cells ... | 1990 | 2345139 |
| pathogenic fungi in soils of jabalpur, india. | results of a preliminary survey of pathogenic fungi in soils collected from forest, riverside and residential garbage soil in jabalpur, india, are reported. a total of 45 soils samples were examined, of which 39 (87%) were positive for fungi, 66 species of fungi classified in 35 genera were isolated: chrysosporium (78%), fusarium (69%), aspergillus (47%), microsporum (38%), gliocladium (16%), acremonium (11%), penicillium (11%), humicola (9%), scopulariopsis (7%), paecilomyces (7%), cladosporium ... | 1990 | 2359416 |
| nitrogen-deregulated mutants of phanerochaete chrysosporium--a lignin-degrading basidiomycete. | two nitrogen-deregulated mutants of phanerochaete chrysosporium, der8-2 and der8-5, were isolated by subjecting wild type conidia to gamma irradiation, plating on poly-r medium containing high levels of nitrogen, and identifying colonies that are able to decolorize poly-r. the mutants showed high levels of ligninolytic activity (14c-synthetic lignin----14co2), and lignin peroxidase, manganese peroxidase and glucose oxidase activities in both low nitrogen (2.4 mm) and high nitrogen (24 mm) media. ... | 1990 | 2369262 |
| characterization of lignin peroxidase-encoding genes from lignin-degrading basidiomycetes. | two closely linked lignin peroxidase (lpo)-encoding genes (lpo) from phanerochaete chrysosporium were isolated. nucleotide sequence studies indicated that the two genes are separated by 1.3 kb of flanking dna and transcribed in opposite directions. cloned p. chrysosporium lpo gene probes have been shown to hybridize to multiple sequences present in the dnas of the white-rot fungi, bjerkandera adusta, coriolus versicolor and fomes lignosus, but no hybridization was detected with dna from pleurotu ... | 1990 | 2373364 |
| biodegradation of tnt (2,4,6-trinitrotoluene) by phanerochaete chrysosporium. | extensive biodegradation of tnt (2,4,6-trinitrotoluene) by the white rot fungus phanerochaete chrysosporium was observed. at an initial concentration of 1.3 mg/liter, 35.4 +/- 3.6% of the [14c]tnt was degraded to 14co2 in 18 days. the addition of glucose 12 days after the addition of tnt did not stimulate mineralization, and, after 18 days of incubation with tnt only, about 3.3% of the initial tnt could be recovered. mineralization of [14c]tnt adsorbed on soil was also examined. ground corncobs ... | 1990 | 2383008 |
| a novel enzymatic decarboxylation of oxalic acid by the lignin peroxidase system of white-rot fungus phanerochaete chrysosporium. | oxidation of veratryl alcohol by lignin peroxidase (lip) was potently inhibited by oxalic acid. the inhibition analysis with lineweaver-burk plots clearly showed that the type of inhibition is non-competitive. the enzymatic oxidation of veratryl alcohol in the presence of 14c-oxalic acid yielded radioactive carbon dioxide. the results indicate that the apparent inhibition of lip is caused by reduction of the veratryl alcohol cation radical intermediate back to the substrate level by oxalate, whi ... | 1990 | 2387411 |
| effect of mn(ii) on reactions catalyzed by lignin peroxidase from phanerochaete chrysosporium. | the effect of manganese on lignin peroxidase activity was studied. the enzyme was produced with a new process using an air-lift-type reactor. the experiments were performed with veratryl alcohol and a dimeric lignin model compound. it was shown that when mn(ii).lactate complex was present the amount of veratraldehyde formed and the uptake of oxygen were significantly enhanced during the aerobic oxidation of veratryl alcohol. a similar effect can be obtained with superoxide dismutase. these resul ... | 1990 | 2401291 |
| selection and characterization of mutants of phanerochaete chrysosporium exhibiting ligninolytic activity under nutrient-rich conditions. | synthesis of the ligninolytic system of the wood-degrading fungus phanerochaete chrysosporium is induced during secondary metabolism, brought about by nitrogen, carbon, or sulfur starvation. we describe here a strategy for selection of mutants which are ligninolytic (lignin----co2) and overproduce lignin-degrading enzymes (ligninases) under nutrient-rich conditions (during primary metabolism). the strategy is based on using an adduct of lysine and a lignin model compound. ligninase-dependent oxi ... | 1990 | 2403260 |
| keratinolytic and keratinophilic fungi of children's sandpits in the city of turin. | soils rich in keratinic residues constitute a permanent or occasional reservoir for dermatophytes and keratinolytic and keratinophilic fungi, and are a source of potential infection for man and animals. the keratinolytic and keratinophilic mycoflora of 28 sandpits in turin was studied to evaluate the risk of fungal skin infections that is run by children as they play in public parks and to lay the ground-work for a next epidemiological study of soil- and animal-inhabiting dermatophytes found in ... | 1986 | 2429185 |
| homology among multiple extracellular peroxidases from phanerochaete chrysosporium. | the extracellular peroxidases of phanerochaete chrysosporium were separated into 21 proteins by analytical isoelectric focusing. fifteen of these enzymes oxidized veratryl alcohol (lignin peroxidases) in the presence of h2o2. six enzymes were mn(ii)-dependent peroxidases. the mn(ii)-dependent enzymes appeared and reached their maximal activity earlier than the lignin peroxidases in the cultures. peptide mapping, amino acid analysis, and reaction against specific antibodies showed that all the mn ... | 1987 | 2432065 |
| genetic factors influencing lignin peroxidase activity in phanerochaete chrysosporium me446. | haploid recombinant progeny of phanerochaete chrysosporium me446, genome compositions of which had been defined by rflp-mapping, vary in their idiophasic behaviour. this allowed us to formulate a model of the sequence of idiophasic activities. one component of this variation, the amount of lignin peroxidase activity, is independent of the allele distributions of the lignin peroxidase gene clusters, but correlates with the allele distribution of another locus. this locus appears to control the sp ... | 1989 | 2571914 |
| dna probe for the identification of histoplasma capsulatum. | a 1.85-kilobase hindiii nuclear dna probe from histoplasma capsulatum g217b detected polymorphic restriction fragments within whole-cell dna from different clinical isolates of h. capsulatum, consistent with the previous system of classification. the probe failed to hybridize to dna from blastomyces dermatitidis, candida spp., saccharomyces cerevisiae, sepedonium chrysospermum, and chrysosporium keratinophilum under low-stringency conditions and therefore may have value as a diagnostic reagent t ... | 1989 | 2573616 |
| phosphorylation of lignin peroxidases from phanerochaete chrysosporium. identification of mannose 6-phosphate. | many of the extracellular lignin-degrading peroxidases from the wood-degrading fungus phanerochaete chrysosporium are phosphorylated. immunoprecipitation of the extracellular fluid of cultures grown with h2k32po4 with a polyclonal antibody raised against one of the lignin peroxidase isozymes, h8 (pi 3.5), revealed the incorporation of h2k32po4 into lignin peroxidases. analyses of the purified isozymes from labeled cultures by isoelectric focusing showed that, in addition to isozyme h8, lignin pe ... | 1989 | 2584220 |
| adiaspiromycosis treated successfully with ketoconazole. | we describe a case of adiaspiromycosis in a 37-year-old male caucasian. this is the first reported case with diffuse involvement of both lungs in a severely ill patient treated successfully with antifungal chemotherapy. | 1989 | 2795406 |
| keratinophilic fungi of school playgrounds in the nablus area, west bank of jordan. | the distribution of keratinophilic fungi in the soil of 57 school playgrounds in the nablus area was studied with relation to human presence. a total number of 73 species was recovered. thirteen of these species were dermatophytes and closely related fungi. the most common and frequent species of the latter fungi were: arthroderma cuniculi (found in 32.1% of school playgrounds), chrysosporium keratinophilum (32.1%), microsporum gypseum (10.7%), t. terrestre (7.1%), and c. asperatum (7.1%). twent ... | 1989 | 2797112 |
| keratinophilic fungi on sheep hairs from the west bank of jordan. | the mycoflora of the hair in 285 sheep from the west bank of jordan was analysed and the frequency of occurrence and the relative importance value for different fungal species found were calculated. ninety six species which belong to 36 genera were isolated. forty one of these species were either well-known agents of animal and human mycoses (trichophyton verrucosum, t. mentagrophytes, microsporum nanum, m. canis, and others), or have been isolated from human and animal lesions (arthroderma cuni ... | 1989 | 2797114 |
| ecological and physiological studies on fungi associated with human hair. | thirty-seven species attributed to 19 genera of keratinophilic fungi were recovered from 100 human hair samples collected from the assiut governorate. the genera aspergillus followed by penicillium and chrysosporium were frequently isolated from 65, 43 and 30% of the samples respectively. fifteen species and 13 genera of thermophilic and thermotolerant fungi (recovered at 45 degrees c) were identified. the thermotolerant aspergillus fumigatus was frequently encountered and emerged from 82% of th ... | 1988 | 2462534 |
| characterization of two lignin peroxidase clones from phanerochaete chrysosporium. | two cdna clones encoding lignin peroxidase isozymes from phanerochaete chrysosporium have been isolated and characterized. one of the clones, lambda ml-4, encodes isozyme h8 as does the previously reported clone lambda ml-1 [tien, m. and tu, c.-p.d. nature 326 (1987) 520-523; 328, 742]. our data are consistent with lambda ml-1 and lambda ml-4 being allelic variants. the other clone, lambda ml-5, encodes a homologous isozyme. we have also isolated the genomic clone corresponding to lambda ml-4 cd ... | 1989 | 2474293 |
| hair perforation by keratinophilic fungi. | the in vitro hair perforation test as described by ajello and george, was applied to eight species of chrysosporium and one species each of microsporum and trichophyton. six of them were found to perforate hair while four were negative. chr. keratinophilum, m. gypseum and tr. vanbreuseghemii were able to perforate and degrade hair rapidly. the micromorphological changes were also described. | 1989 | 2477700 |
| fungi on the claws of buffalo and cow in egypt. | the frequency of occurrence of fungi in 120 claw samples of buffalo and cow from different localities at assiut governorate was determined. thirty-seven species which belong to twenty genera were collected from the two substrates and the most common genera were chrysosporium (eleven species of chrysosporium were isolated). aspergillus and scopulariopsis. from the preceding genera chrysosporium sulfureum was common on cow claws but c. merdarium was slightly dominant on buffalo claws than on cow c ... | 1989 | 2482351 |
| rflp-based genetic map of phanerochaete chrysosporium me446: lignin peroxidase genes occur in clusters. | we describe the construction of an rflp-based genetic map of the white rot fungus phanerochaete chrysosporium me446. the map is deduced from the allele distributions of 38 rflp markers in a test set of 53 meiotically derived haploid recombinants of this strain. the map includes a cellulase gene, a matingtype locus and a family of lignin peroxidase (and related) genes that are arranged in two unlinked clusters. | 1989 | 2571913 |
| multiple ligninase-related genes from phanerochaete chrysosporium. | we have investigated four distinct sequences in genomic dna of phanerochaete chrysosporium that show strong homology to a lignin peroxidase cdna sequence [tien and tu: nature 236 (1987) 520-523, and 328 (1987) 742]. the complete nucleotide sequence of one of these genes is presented here. comparison of the sequenced gene, lig1, with the published cdna sequence reveals the presence of eight short intervening sequences. the open reading frame encodes a polypeptide of 372 amino acids (aa), which in ... | 1988 | 2907500 |
| fungi carried from farmers' work into farm homes. | airborne fungal spore concentrations and main fungal genera were compared in rural and urban living environments in finland during the winter. in addition to conventional viable fungal spore counts (based on the six-stage impactor sampling and cultivation), total spore concentrations were obtained by scanning electron microscope (sem) investigation of filter samples. the viable spore counts were only 0.2%-25% of the number of total spore aggregates. a high correlation between these two methods w ... | 1989 | 2596402 |
| keratinophilic fungi associated with rabbit claws in egypt. | using the soil plate technique, thirty one species appertaining to twenty three genera were recovered from 160 samples of rabbit claws which were collected from a rabbit farm at assiut. of the true keratinophilic fungi chrysosporium tropicum was the most prevalent where it colonized 56.25% of the samples. microsporum gypseum was rarely isolated in this study. among the non-keratinophilic fungi penicillium funiculosum. p. jenseni and paccilomyces lilacinus were of low incidence (20%, 19.37% and 1 ... | 1989 | 2600778 |
| oxidation-reduction potentials and ionization states of extracellular peroxidases from the lignin-degrading fungus phanerochaete chrysosporium. | the oxidation-reduction potentials of lignin peroxidase isozymes h1, h2, h8, and h10 as well as the mn-dependent peroxidase isozymes h3 and h4 are reported. the potentiometric titrations involving the ferrous and ferric states of the enzyme had nernst plots indicating single-electron transfer. the em7 values of lignin peroxidase isozymes h1, h2, h8, and h10 are -142, -135, -137, and -127 mv versus standard hydrogen electrode, respectively. the em7 values for the mn-dependent peroxidase isozymes ... | 1989 | 2605198 |
| keratinophilic fungi of animal and bird pens in egypt. | samples were collected from 44 pens under animals and birds for the estimation of keratinophilic fungi using the hair-baiting technique. thirty-nine species which belong to eighteen genera were recovered. chrysosporium, aspergillus, and penicillium were the most frequent genera. from keratinophilic fungi, myceliophthora anamorph of corynascus sepedonium, myceliophthora anamorph of corynascus novoguinensis, microsporum boullardii, m. gypseum, trichophyton metagrophytes and arthroderma sp. were re ... | 1989 | 2614672 |
| isolation of keratinophilic fungi from the dust of ferry boats and trains in italy. | the occurrence of fungi of the genera epidermophyton, microsporum, trichophyton and chrysosporium in dust collected from 10 ferry boats and 11 railway cars in italy was studied. ferry boats: 101 colonies of fungi were isolated. of the dermatophytes, e. floccosum represented 2.0% of all the colonies isolated, m. canis 3.0%, m. gypseum 5.0% and tr. mentagrophytes represented 2.0%. e. floccosum was isolated from 2 ferry boats, m. canis from 3, m. gypseum from 4 and tr. mentagrophytes from 2. only t ... | 1989 | 2615785 |
| dermatophytes and keratinophilic fungi in cats and dogs. | during an 11 month period from january to november 1987, dermatophytes and keratinophilic fungi were isolated from 70 (75%) of 93 cats and 62 (36.9%) of 168 dogs. the most common isolate was microsporum canis from 54 (58%) cats and 33 (19.6%) dogs, followed by trichophyton mentagrophytes from 5 (5.4%) cats and 9 (5.3%) dogs, aphanoascus fulvescens (4 cats and 9 dogs), m. gypseum (5 dogs), tr. terrestre (3 dogs) myceliophthora vellerea (2 dogs) and chrysosporium indicum, chr. merdarium and chr. t ... | 1989 | 2622475 |
| [a fatal case of human pulmonary adiaspiromycosis]. | a fatal case of human pulmonary adiaspiromycosis is reported. the patient, a male farm laborer from pernambuco, in the northeastern part of brazil, had moved to planaltina, df., one year prior the onset of his illness. the main clinical manifestations consisted in fever, myalgia, cough and dyspnea. after being sick for five weeks, the patient developed severe respiratory failure and died. grossly, disseminated nodular lesions, a few millimeters in diameter, were observed throughout the lobes of ... | 1989 | 2694306 |
| biodegradation of polycyclic hydrocarbons by phanerochaete chrysosporium. | the ability of the white rot fungus phanerochaete chrysosporium to degrade polycyclic aromatic hydrocarbons (pahs) that are present in anthracene oil (a distillation product obtained from coal tar) was demonstrated. analysis by capillary gas chromatography and high-performance liquid chromatography showed that at least 22 pahs, including all of the most abundant pah components present in anthracene oil, underwent 70 to 100% disappearance during 27 days of incubation with nutrient nitrogen-limite ... | 1989 | 2705768 |
| trametes versicolor ligninase: isozyme sequence homology and substrate specificity. | the substrate specificity of three ligninase isozymes from the white-rot fungus trametes versicolor has been investigated using stereochemically defined synthetic dimeric models for lignin. the isozymes have been found to attack non-phenolic beta-o-4 as well as beta-1 lignin model compounds. this finding confirms the classification of the isozymes from t. versicolor as ligninases. the amino-terminal residues of the three isozymes from t. versicolor have been determined using edman degradation. m ... | 1989 | 2707445 |
| keratinolytic fungi of wadi qena in egypt. | forty-six soil samples collected from different sites of wadi qena were examined for keratinophilic fungi using the hair baiting technique. thirty-two species in addition to one variety of each of a. nidulans and a. flavus which belong to eighteen genera were recovered. aspergillus, chrysosporium, penicillium, microsporum and fusarium were the most frequent genera developed from baited soils. | 1989 | 2707686 |
| use of a shuttle vector for the transformation of the white rot basidiomycete, phanerochaete chrysosporium. | a novel shuttle vector based spheroplast transformation system for the lignin degrading filamentous fungus p. chrysosporium is described. the transformation vector, designated prr12, consists of the yeast integration plasmid yip5, a putative autonomous replication sequence (ars) of p. chrysosporium, and a 2.2 kb pvuii fragment carrying kanr determinant from plasmid png35, which confers resistance against both kanamycin and the related antibiotic g418. two different strains of p. chrysosporium (m ... | 1989 | 2735919 |
| infection of the central nervous system by rhinocladiella atrovirens in a patient with acquired immunodeficiency syndrome. | a case of central nervous system invasion by rhinocladiella atrovirens is described. the patient was an intravenous drug abuser suffering from acquired immunodeficiency syndrome. | 1989 | 2746436 |
| manganese-dependent peroxidase from phanerochaete chrysosporium. primary structure deduced from cdna sequence. | a cdna clone encoding a manganese-dependent peroxidase from the filamentous fungus phanerochaete chrysosporium was isolated and characterized. the clone, lambda mp-1, was isolated by screening a lambda gt11 expression library with polyclonal antibodies raised against a purified manganese-dependent peroxidase (isozyme h4, pi 4.5). the lambda mp-1 cdna sequence predicts a mature protein containing 358 amino acids with a molecular weight of 37,711 preceded by a leader peptide of 24 amino acid resid ... | 1989 | 2760033 |
| thiol-mediated oxidation of nonphenolic lignin model compounds by manganese peroxidase of phanerochaete chrysosporium. | in the presence of mnii, h2o2, and glutathione (gsh), manganese peroxidase oxidized veratryl alcohol (i) to veratraldehyde (iv). anisyl alcohol (ii) and benzyl alcohol (iii) were also oxidized by this system to their corresponding aldehydes (v and vi). in the presence of gsh, chemically prepared mniii or gamma-irradiation also catalyzed the oxidation of i, ii, and iii to iv, v, and vi, respectively. gsh and dithiothreitol rapidly reduced mniii to mnii in the absence of aromatic substrates and th ... | 1989 | 2760063 |
| keratinophilic fungi from the duck nails in egypt. | the mycoflora of one hundred and sixty of duck nail samples, collected from the duck farm, faculty of agriculture, assiut university, were examined. using the soil plate technique and sabouraud's dextrose agar, thirty six species appertaining to twenty genera of keratinophilic fungi were isolated. chrysosporium was the genus most frequently isolated (50% of the samples). aspergillus and penicillium occurred in low incidences (13.1 and 12.5% of total samples). trichophyton rubrum was found to col ... | 1989 | 2778647 |
| distribution of keratinophilic fungi in desert soil of kuwait. | forty soil samples from different desert localities in kuwait were surveyed for keratinophilic and geophilic dermatophytic fungi. sixteen species classified in 9 genera were recovered on wool baits. chrysosporium species were the most common fungi: chr. tropicum (60%), chr. pannicola (32.5%), chr. queenslandicum (27.5%), chr. keratinophilum (25%), chr. indicum (20%) and the chrysosporium anamorph of arthroderma cuniculi. the second dominant genus was malbranchea (27%) represented by two species; ... | 1989 | 2779610 |
| cellulase families revealed by hydrophobic cluster analysis. | the amino acid sequences of 21 beta-glycanases have been compared by hydrophobic cluster analysis. six families of cellulases have been identified on the basis of primary structure homology: (a) endoglucanases b, c and e of clostridium thermocellum; endoglucanases of erwinia chrysanthemi and bacillus sp.; endoglucanase iii of trichoderma reesei; endoglucanase i of schizophyllum commune; (b) cellobiohydrolase ii of t. reesei; endoglucanases of cellulomonas fimi and streptomyces sp; (c) cellobiohy ... | 1989 | 2806912 |
| pathogenicity of some chrysosporium species isolated in france. | in order to appreciate the pathogenicity of several geophilic chrysosporium species (including anixiopsis stercoraria, chrysosporium keratinophilum, c. tropicum, c. pannorum, c. state arthroderma curreyi, c. state of a. multifidum, and c. state of a. tuberculatum), the authors have realized two series of experimental infestations. inoculation of these fungi on the back of guinea pigs produced rare erythematous scaling lesions which spontaneously disappeared 3-5 weeks later. no real hair invasion ... | 1989 | 2811980 |
| spectral characterization of manganese peroxidase, an extracellular heme enzyme from the lignin-degrading basidiomycete, phanerochaete chrysosporium. | manganese peroxidase (mnp) is a component of the lignin degradation system of the basidiomycetous fungus, phanerochaete chrysosporium. this novel mnii-dependent extracellular enzyme (mr = 46,000) contains a single protoporphyrin ix prosthetic group and oxidizes phenolic lignin model compounds as well as a variety of other substrates. to elucidate the heme environment of this enzyme, we have studied its electron paramagnetic resonance and resonance raman spectroscopic properties. these studies in ... | 1988 | 2835361 |
| structure and regulation of a lignin peroxidase gene from phanerochaete chrysosporium. | we have been investigating the structure and regulation of the lignin peroxidase genes from phanerochaete chrysosporium. the gene for lignin peroxidase isozyme h8 was isolated and sequenced. the gene is split into nine exons and eight introns. the introns have consensus sequences both at the splice junctions and at an internal element which may be involved in the splicing mechanism. the promoter contains the eukaryotic consensus elements: a tata box at -78 and a acaat box at -106. transcription ... | 1988 | 2844176 |
| glucose oxidase of phanerochaete chrysosporium. | | 1988 | 3226296 |
| ligninase-mediated phenoxy radical formation and polymerization unaffected by cellobiose:quinone oxidoreductase. | phanerochete chrysosporium ligninase (+ h2o2) oxidized the lignin substructure-related compound acetosyringone to a phenoxy radical which was identified by esr spectroscopy. cellobiose:quinone oxidoreductase (cbqase) + cellobiose, previously suggested to be a phenoxy radical reducing system, was without effect on the radical. ligninase polymerized guaiacol and it increased the molecular size of a synthetic lignin. these polymerizations, reflecting phenoxy radical coupling reactions, were also un ... | 1988 | 2844307 |
| molecular analysis of a phanerochaete chrysosporium lignin peroxidase gene. | the basidiomycete fungus phanerochaete chrysosporium produces a number of extracellular peroxidases which appear to be important for lignin degradation. we present here the isolation and complete nucleotide (nt) sequence of a gene (lpo) coding for lignin peroxidase (lpo), the coding region of which is identical to a lpo cdna sequence which had previously been described [m. tien and c.-p.d. tu, nature 326 (1987) 520-523]. the deduced amino acid (aa) sequence corresponds to 372 aa residues and the ... | 1988 | 3240864 |
| cleavages of aromatic ring and beta-o-4 bond of synthetic lignin (dhp) by lignin peroxidase. | lignin peroxidase from a white-rot basidiomycete, phanerochaete chrysosporium, catalyzed cleavages of the aromatic ring and the beta-o-4 bond of a synthetic lignin, a dehydrogenation copolymer (dhp) of coniferyl alcohol and a (beta-o-4)-(beta-beta) lignin substructure model trimer. | 1989 | 2914615 |
| manganese peroxidase from the lignin-degrading basidiomycete phanerochaete chrysosporium. transient state kinetics and reaction mechanism. | stopped-flow techniques were used to investigate the kinetics of the formation of manganese peroxidase compound i (mnpi) and of the reactions of mnpi and manganese peroxidase compound ii (mnpii) with p-cresol and mnii. all of the rate data were obtained from single turnover experiments under pseudo-first order conditions. in the presence of h2o2 the formation of mnpi is independent of ph over the range 3.12-8.29 with a second-order rate constant of (2.0 +/- 0.1) x 10(6) m-1 s-1. the activation e ... | 1989 | 2914954 |
| molecular cloning and sequences of lignin peroxidase genes of phanerochaete chrysosporium. | the genomic clones encoding lignin peroxidase isozyme h8 and two closely related genes were isolated from phanerochaete chrysosporium bkm-1767, and their nucleotide sequences were determined. the positions and approximate lengths of introns were found to be highly conserved in all three clones. analysis of homokaryotic derivatives indicated that the three clones are not alleles of the same gene(s). | 1989 | 2761543 |
| keratinophilic fungi isolated from antarctic soil. | in the present study, 10 soil samples were collected aseptically from an equal number of areas of the antarctic in the zone occupied by the 1986-1987 italian expedition for research on keratinophilic fungi. of particular interest was the isolation of a pathogenic fungus, microsporum gypseum, from two sites in the base camp occupied by men and by skuas. trichophyton terrestre was isolated from a site in which people worked and through which penguins and skuas passed. the most widespread fungal sp ... | 1989 | 2770838 |
| isolation of keratinophilic fungi from floor dust in arab elementary and preparatory schools in the west bank of jordan. | floor dust collected from classrooms of thirty three elementary schools (16 for girls, and 17 for boys) (children aged 6-11), and twenty four preparatory schools (13 for girls, and 11 for boys) (children aged 12-14) was studied for the occurrence of keratinophilic fungi with respect to human presence and age of children. tichophyton mentagrophytes was present in 15.4% of the preparatory schools for girls, in 12.5% of elementary schools for girls, and in 11.8% of elementary schools for boys. it w ... | 1989 | 2770839 |
| [biological, chemical and pathohistologic study of the skin in papular dermatitis in fattened pigs]. | we examined the skin samples from 23 fattened pigs with papular dermatitis (pd) and from 17 pigs showing no skin lesions; the live weight of the pigs was from 95 to 105 kg and the pigs came from a large fattening house d that is located in the region where the mines are frequent (lignite extraction). it was only in 26% of pigs with pd where the common ectoparasites haematopinus suis and sarcoptes scabiei var. suis were found out. the skin lesions were not fluorescent in the light of wood's lamp. ... | 1986 | 3095976 |
| [the practical application of exoantigen tests]. | | 1988 | 3131672 |
| manganese peroxidase from the basidiomycete phanerochaete chrysosporium: spectral characterization of the oxidized states and the catalytic cycle. | manganese peroxidase (mnp), an extracellular heme enzyme from the lignin-degrading fungus phanerochaete chrysosporium, catalyzes the mn(ii)-dependent oxidation of a variety of phenols. herein, we spectroscopically characterize the oxidized states of mnp compounds i, ii, and iii and clarify the role of mn in the catalytic cycle of the enzyme. addition of 1 equiv of h2o2 to the native ferric enzyme yields compound i, characterized by absorption maxima at 407, 558, 605, and 650 nm. addition of 2 or ... | 1988 | 3167051 |
| deep mycoses in amazon region. | patients with deep mycoses diagnosed in dermatologic clinics of manaus (state of amazonas, brazil) were studied from november 1973 to december 1983. they came from the brazilian states of amazonas, pará, acre, and rondônia and the federal territory of roraima. all of these regions, with the exception of pará, are situated in the western part of the amazon basin. the climatic conditions in this region are almost the same: tropical forest, high rainfall, and mean annual temperature of 26c. the dee ... | 1988 | 3220630 |
| biodegradation of pentachlorophenol by the white rot fungus phanerochaete chrysosporium. | extensive biodegradation of pentachlorophenol (pcp) by the white rot fungus phanerochaete chrysosporium was demonstrated by the disappearance and mineralization of [14c]pcp in nutrient nitrogen-limited culture. mass balance analyses demonstrated the formation of water-soluble metabolites of [14c]pcp during degradation. involvement of the lignin-degrading system of this fungus was suggested by the fact the time of onset, time course, and eventual decline in the rate of pcp mineralization were sim ... | 1988 | 3223759 |
| mycoflora of air-conditioners dust from riyadh, saudi arabia. | using the hair baiting technique, 6 genera and 14 species were collected on sabouraud's dextrose agar from 37 dust samples from air-conditioners. the most common fungi were chrysosporium tropicum, c. indicum, c. keratinophilum, aspergillus flavus followed by acremonium strictum and scopulariopsis brevicaulis. using the dilution-plate method, 26 genera and 52 species were collected from 37 dust samples on glucose-(23 genera and 45 species) and cellulose-(18 genera and 34 species) czapek's agar at ... | 1988 | 3236219 |
| the identification, molecular cloning and characterisation of a gene from phanerochaete chrysosporium that shows strong homology to the exo-cellobiohydrolase i gene from trichoderma reesei. | we have identified a genomic dna fragment from the lignin-degrading fungus phanerochaete chrysosporium (p.c.) that hybridizes to a dna probe encoding part of the exo-cellobiohydrolase i (cbhi) gene of trichoderma reesei (t.r.). this fragment has been subcloned and its nucleotide sequence determined. we demonstrate that it could encode a 516 residue protein that shows strong homology with the known protein sequence of cbhi from t.r. comparison of the two nucleotide sequences identifies two region ... | 1988 | 3246351 |
| [study on psychrophilic/psychrotrophic microorganisms. ii. microbes in cold-storage food]. | | 1988 | 3250095 |
| characterization of a cdna encoding a manganese peroxidase, from the lignin-degrading basidiomycete phanerochaete chrysosporium. | a cdna clone of a manganese peroxidase (mnp) from phanerochaete chrysosporium was isolated and characterized. the cdna contains 1314 nucleotides excluding the poly(a) tail and the coding region has 68% g + c content. the deduced mature mnp protein contains 357 amino acids and is preceded by a 21-amino acid leader sequence. the experimentally determined n-terminal sequence of the purified mnp-1 protein, pi = 4.9, corresponds to the deduced n-terminal sequence of the gene. the mr of the mature mnp ... | 1989 | 2925681 |
| endocarditis due to chrysosporium species: a disease of medical progress? | | 1986 | 2936831 |
| the ligninase of phanerochaete chrysosporium generates cation radicals from methoxybenzenes. | the hemoprotein ligninase of phanerochaete chrysosporium catalyzes, in the presence of h2o2, a variety of seemingly different oxidations in lignin and lignin model compounds. here we show that the enzyme also catalyzes the oxidation of various methoxybenzenes. esr spectroscopy shows that the compounds are oxidized to aryl cation radicals. these decompose, evidently by h2o addition. thus, 1,4-dimethoxybenzene is converted to p-benzoquinone and methanol. we propose a unified mechanism, based on fo ... | 1985 | 2982828 |
| keratinophilic fungi from nigerian soil. | during a survey of keratinophilic fungi in nigerian soil samples, twelve species of fungi were isolated. these included chrysosporium keratinophilum, c. tropicum, curvularia lunata, microsporum audouinii, m. canis, m. fulvum, m. gypseum, m. vanbreuseghemii, trichophyton ajelloi, t. mentagrophytes, t. soudanense and t. yaoundei. these keratinophilic fungi had a higher incidence by occurrence of individual species during the rainy season (may-october) than the dry season (november-april). | 1987 | 3657901 |
| free hydroxyl radical is not involved in an important reaction of lignin degradation by phanerochaete chrysosporium burds. | hydroxyl radical (ho.) has been implicated in the degradation of lignin by phanerochaete chrysosporium. this study assessed the possible involvement of ho. in degradation of lignin substructural models by intact cultures and by an extracellular ligninase isolated from the cultures. two non-phenolic lignin model compounds [aryl-c(alpha)hoh-c(beta)hr-c(gamma)h2oh, in which r = aryl (beta-1) or r = o-aryl (beta-o-4)] were degraded by cultures, by the purified ligninase, and by fenton's reagent (h2o ... | 1985 | 2986597 |
| spectral characterization of diarylpropane oxygenase, a novel peroxide-dependent, lignin-degrading heme enzyme. | diarylpropane oxygenase, an h2o2-dependent lignin-degrading enzyme from the basidiomycete fungus phanerochaete chrysosporium, catalyzes the oxygenation of various lignin model compounds with incorporation of a single atom of dioxygen (o2). diarylpropane oxygenase is also capable of oxidizing some alcohols to aldehydes and/or ketones. this enzyme (mr = 41,000) contains a single iron protoporphyrin ix prosthetic group. previous studies revealed that the soret maximum of the ferrous-co complex of d ... | 1985 | 2987213 |
| mechanism of oxidative c alpha-c beta cleavage of a lignin model dimer by phanerochaete chrysosporium ligninase. stoichiometry and involvement of free radicals. | the hemoprotein ligninase of phanerochaete chrysosporium burds. catalyzes the oxidative cleavage of lignin model dimers between c alpha and c beta of their propyl side chains. the model dimers hitherto used give multiple products and complex stoichiometries upon enzymatic oxidation. here we present experiments with a new model dimer, 1-(3,4-dimethoxyphenyl)-2-phenylethanediol (dimethoxyhydrobenzoin, dmhb) which is quantitatively cleaved by ligninase in air to give benzaldehyde and veratraldehyde ... | 1985 | 2989288 |
| steady-state and transient-state kinetic studies on the oxidation of 3,4-dimethoxybenzyl alcohol catalyzed by the ligninase of phanerocheate chrysosporium burds. | catalysis of the h2o2-dependent oxidation of 3,4-dimethoxybenzyl (veratryl) alcohol by the hemoprotein ligninase isolated from wood-decaying fungus, phanerochaete chrysosporium burds, is characterized. the reaction yields veratraldehyde and exhibits a stoichiometry of one h2o2 consumed per aldehyde formed. ping-pong steady-state kinetics are observed for h2o2 (km = 29 microm) and veratryl alcohol (km = 72 microm) at ph 3.5. the magnitude of the turnover number varies from 2 to 3 s-1 at this ph, ... | 1986 | 3003081 |
| substrate free radicals are intermediates in ligninase catalysis. | the h2o2-requiring ligninase of the basidiomycete phanerochaete chrysosporium oxidatively cleaves both lignin and lignin model compounds between c alpha and c beta (c-1 and c-2) of their aliphatic side chains. previous work has demonstrated a reaction mechanism by which ligninase oxidizes aromatic substrates to their cation radicals, which then undergo side chain cleavage to yield carbon-centered free radicals. these carbon-centered radicals add o2 to give substrate peroxyl radicals that react f ... | 1986 | 3012530 |
| h2o2-dependent decolorization of poly r-481 by particulate fractions from phanerochaete chrysosporium. | a cell-free preparation from phanerochaete chrysosporium culture medium decolorized the polymeric dye poly r-481. the majority of this decolorization activity sedimented when centrifuged at 150,000 x g, indicating that it was associated with a particulate body. the activity was sensitive to heat, azide and cyanide, was stimulated by exogenously added h2o2, and was optimal around ph 4. electron micrographs of the sedimented culture medium fraction showed the presence of numerous particulate struc ... | 1986 | 3707574 |
| oxidation of polycyclic aromatic hydrocarbons and dibenzo[p]-dioxins by phanerochaete chrysosporium ligninase. | the lignin peroxidase (ligninase) of phanerochaete chrysosporium catalyzes the oxidation of a variety of lignin-related compounds. here we report that this enzyme also catalyzes the oxidation of certain aromatic pollutants and compounds related to them, including polycyclic aromatic hydrocarbons with ionization potentials less than or equal to approximately 7.55 ev. this result demonstrates that the h2o2-oxidized states of lignin peroxidase are more oxidizing than the analogous states of classic ... | 1986 | 3023375 |
| ligninase of phanerochaete chrysosporium. mechanism of its degradation of the non-phenolic arylglycerol beta-aryl ether substructure of lignin. | this study examined the ligninase-catalysed degradation of lignin model compounds representing the arylglycerol beta-aryl ether substructure, which is the dominant one in the lignin polymer. three dimeric model compounds were used, all methoxylated in the 3- and 4-positions of the arylglycerol ring (ring a) and having various substituents in the beta-ether-linked aromatic ring (ring b), so that competing reactions involving both rings could be compared. studies of the products formed and the tim ... | 1986 | 3024619 |
| lignin peroxidase: resonance raman spectral evidence for compound ii and for a temperature-dependent coordination-state equilibrium in the ferric enzyme. | resonance raman (rr) spectroscopy of lignin peroxidase (ligninase, dairylpropane oxygenase) from the basidiomycete phanerochaete chrysosporium suggests two different coordination states for the native ferric enzyme. evidence for a high-spin, hexacoordinate ferric protoporphyrin ix was presented by andersson et al. [andersson, l. a., renganathan, v., chiu, a.a., loehr, t. m., & gold, m. h. (1985) j. biol. chem. 260, 6080-6087], whereas kuila et al. [kuila, d., tien, m., fee, j. a., & ondrias, m. ... | 1987 | 3040086 |
| inhibitory activity of the genus chrysosporium in relation to the composition of the culture media used. | the effect of nutrients on the formation and accumulation of secondary metabolites in species of the genus chrysosporium was examined. the study was performed according to the method of campbell (1960), and among the results obtained was the fact that the only significant increase in antimicrobial capacity was observed when the strains were grown on a 2% malt extract agar and when micro-organisms were assayed using aspergillus niger. | 1986 | 3736442 |
| veratryl alcohol oxidases from the lignin-degrading basidiomycete pleurotus sajor-caju. | the basidiomycete pleurotus sajor-caju mineralizes ring-14c-labelled lignin (dehydrogenative polymer) when grown in mycological broth. under these conditions, two veratryl alcohol oxidase (vao) enzymes were found in the culture medium. they oxidized a number of aromatic alcohols to aldehydes and reduced o2 to h2o2. the enzymes were purified by ion-exchange and gel-permeation chromatography. the final step of purification on mono q resolved the activity into two peaks (vao i and vao ii). both enz ... | 1988 | 3060110 |
| comparison of ligninase-i and peroxidase-m2 from the white-rot fungus phanerochaete chrysosporium. | ligninase-i (mr 42,000-43,000; carbohydrate, 21%) and peroxidase-m2 (mr 45,000-47,000; carbohydrate, 17%), two representative, hydrogen peroxide-dependent extracellular enzymes produced by ligninolytic cultures of the white-rot fungus phanerochaete chrysosporium bkm-f-1767, were purified and their properties compared. spectroscopic studies showed that both native enzymes are heme proteins containing protoporphyrin ix. epr spectroscopy indicated that iron ions are coordinated with the enzymes' pr ... | 1986 | 3080953 |
| [survey of keratinophilic fungi from soils and mating behaviour of microsporum canis from human ringworm cases in hokkaido prefecture in japan]. | from 383 (38.3%) of a total 1000 of soil samples, collected from hokkaido prefecture, keratinophilic fungi have been isolated using hair baiting technique. the fungi isolated were as follows: 318 strains of trichophyton ajelloi (including 90 strains of arthroderma uncinatum), 59 of microsporum cookei (including 13 of nannizzia cajetani), 32 of chrysosporium keratinophilum, 23 of c. tropicum, 15 of chrysosporium anamorph of a. tuberculatum, 6 of t. terrestre (including 3 of a. insingulare and one ... | 1988 | 3384396 |
| biodegradation of crystal violet by the white rot fungus phanerochaete chrysosporium. | biodegradation of crystal violet (n,n,n',n',n'',n''-hexamethylpararosaniline) in ligninolytic (nitrogen-limited) cultures of the white rot fungus phanerochaete chrysosporium was demonstrated by the disappearance of crystal violet and by the identification of three metabolites (n,n,n',n',n''-pentamethylpararosaniline, n,n,n',n''-tetramethylpararosaniline, and n,n',n''-trimethylpararosaniline) formed by sequential n-demethylation of the parent compound. metabolite formation also occurred when crys ... | 1988 | 3389809 |
| spectroscopic and kinetic properties of the oxidized intermediates of lignin peroxidase from phanerochaete chrysosporium. | stopped-flow rapid scan techniques were used to obtain a spectrum of nearly homogeneous lignin peroxidase compound i (lipi) under pseudo-first order conditions at the unusually low ph optimum (3.0) for the enzyme. the lipi spectrum had a soret band at 407 nm with approximately 60% reduced intensity and a visible maximum at 650 nm. under steady-state conditions a soret spectrum for lignin peroxidase compound ii (lipii) was also obtained. the soret maximum of lipii at 420 nm was only approximately ... | 1988 | 3392025 |
| analysis of nucleotide sequences of two ligninase cdnas from a white-rot filamentous fungus, phanerochaete chrysosporium. | an analysis of nucleotide sequences of two types of ligninase cdnas isolated from the basidiomycete phanerochaete chrysosporium, designated clg4 and clg5, are presented here. the amino acid sequences of the corresponding ligninase proteins, designated lg4 and lg5, respectively, have been deduced from the cdna sequences. mature ligninases lg4 and lg5 are preceded by leader sequences containing 28 and 27 amino acids (aa), respectively, and each contains 344 aa residues. the estimated mrs of mature ... | 1987 | 3440521 |
| ethanol production in table jelly by two species of chrysosporium. | different commercial brands of tablet jelly were stored in sealed containers for 12 weeks at an initial water activity of 0.90. the surface of those containing real fruit juice became covered with a dense white mould identified as chrysosporium inops. that of those without fruit juice did not. this type of spoilage has been observed in commercially stored samples. when spores of this fungus and the closely related species, c. xerophilum, were added to commercial tablet jelly, the fungi grew only ... | 1987 | 3440763 |
| [keratinophilic fungi in the slime of the artificial lake pławniowice]. | | 1987 | 3445091 |
| involvement of a new enzyme, glyoxal oxidase, in extracellular h2o2 production by phanerochaete chrysosporium. | the importance of extracellular h2o2 in lignin degradation has become increasingly apparent with the recent discovery of h2o2-requiring ligninases produced by white-rot fungi. here we describe a new h2o2-producing activity of phanerochaete chrysosporium that involves extracellular oxidases able to use simple aldehyde, alpha-hydroxycarbonyl, or alpha-dicarbonyl compounds as substrates. the activity is expressed during secondary metabolism, when the ligninases are also expressed. analytical isoele ... | 1987 | 3553159 |
| description of a case of rhinosporidiosis in spain. | rhinosporidiosis is reviewed, and the first autochthonous case in spain is presented (site: in the nasal cavity of a 19-year-old male from a rural background). diagnosis was established morphologically after eliminating the possibilities of cryptococcus neoformans, coccidioides immitis, and chrysosporium crescens. clinico-pathological features are described. preparations were stained with hematoxylin-eosin, pas, and methenamine silver, and studied for fluorescence. certain aspects of the epidemi ... | 1987 | 3561472 |
| purification and characterization of glucose oxidase from ligninolytic cultures of phanerochaete chrysosporium. | glucose oxidase, an important source of hydrogen peroxide in lignin-degrading cultures of phanerochaete chrysosporium, was purified to electrophoretic homogeneity by a combination of ion-exchange and molecular sieve chromatography. the enzyme is a flavoprotein with an apparent native molecular weight of 180,000 and a denatured molecular weight of 80,000. this enzyme does not appear to be a glycoprotein. it gives optimal activity with d-glucose, which is stoichiometrically oxidized to d-gluconate ... | 1986 | 3957868 |
| properties of ligninase from phanerochaete chrysosporium and their possible applications. | the wood-degrading fungus phanerochaete chrysosporium burds produces a family of enzymes which degrade lignin and lignin-like substrates. these ligninases exhibit a high degree of homology in being hemeprotein peroxidases, in mr, in cross reactivity to polyclonal antibodies, in being glycosylated, and in catalytic properties. the predominant ligninase is able to generate cation radicals in its aromatic substrates. these radicals can undergo a variety of reactions thus explaining the nonspecific ... | 1987 | 3322681 |
| cloning and sequencing of a cdna for a ligninase from phanerochaete chrysosporium. | lignin is a complex polymer of phenylpropanoid subunits. it is an essential component of woody tissue, to which it imparts structural rigidity. lignin is remarkably resistant to degradation by most microbes; nevertheless, a few species of white-rot fungi are able to catalyse its oxidation to co2. its biodegradation is of great ecological significance because, next to cellulose, lignin is the most abundant renewable polymer on earth. the first step in lignin degradation is depolymerization, catal ... | 1987 | 3561490 |
| increased synthesis of ajmalicine and catharanthine by cell suspension cultures of catharanthus roseus in response to fungal culture-filtrates. | the ammonium sulfate-precipitated fraction from mycelia and culture-filtrates and the crude, cell-free culture filtrates from the growth medium of the fungi chrysosporium palmorum, eurotium rubrum, micromucor isabellina, and pythium aphanidermatum when aseptically added to cell suspensions of cantharanthus roseus caused a rapid and dramatic increase in indole alkaloid biosynthesis. up to 400 micrograms/l ajmalicine and 600 micrograms/l catharanthine were detected in c. roseus cell suspension gro ... | 1987 | 3619437 |
| [keratinophilic fungal flora isolated from small wild mammals and rabbit-warren in france. discussion on the fungal species found]. | the occurrence of dermatophytes and other keratinophilic fungi was investigated in 237 small wild mammals and 125 european rabbit. the purpose of the investigation was to determine what were the species of fungi present in the these animals. four species of dermatophytes were isolated: trichophyton ajelloi, trichophyton terrestre, trichophyton mentagrophytes, microsporum persicolor. trichophyton terrestre was the most frequently isolated and it occurred more frequently than its presence could be ... | 1987 | 3662337 |
| purification and characterization of an extracellular mn(ii)-dependent peroxidase from the lignin-degrading basidiomycete, phanerochaete chrysosporium. | a mn(ii)-dependent peroxidase found in the extracellular medium of ligninolytic cultures of the white rot fungus, phanerochaete chrysosporium, was purified by deae-sepharose ion-exchange chromatography, blue agarose chromatography, and gel filtration on sephadex g-100. sodium dodecyl sulfate-gel electrophoresis indicated that the homogeneous protein has an mr of 46,000. the absorption spectrum of the enzyme indicates the presence of a heme prosthetic group. the pyridine hemochrome absorption spe ... | 1985 | 4062285 |
| biodegradation of ddt [1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane] by the white rot fungus phanerochaete chrysosporium. | extensive biodegradation of 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (ddt) by the white rot fungus phanerochaete chrysosporium was demonstrated by disappearance and mineralization of [14c]ddt in nutrient nitrogen-deficient cultures. mass balance studies demonstrated the formation of polar and water-soluble metabolites during degradation. hexane-extractable metabolites identified by gas chromatography-mass spectrometry included 1,1,-dichloro-2,2-bis(4-chlorophenyl)ethane (ddd), 2,2,2-trichlo ... | 1987 | 3674869 |
| detection of lectin from chrysosporium keratinophilum (frey) carmichael and anixiopsis stercoraria (hansen) hansen by inhibition of haemagglutination. | several strains of chrysosporium keratinophilum and anixiopsis stercoraria agglutinate the red blood cells of different origins (man, sheep, rabbit, mouse). the best agglutination was obtained with rabbit erythrocytes. the inhibition experiments by carbohydrate solutions (oses, osamines and glycoproteins) were performed with 3 haemagglutination units of these fungi and revealed the lectin nature of this haemagglutinin, as evidenced by the specific inhibiting activity of n-acetyl-neuraminic acid. ... | 1986 | 3689587 |
| [87. isolation of keratinophilic fungi from the soil using the hair-lure method. determination of microsporum gypseum (nannizia incurvata) and trichophyton terrestre from the austrian soil, keratinomyces ajelloi from the danish soil and chrysosporium keratinophilum from japanese soil]. | | 1972 | 4673848 |
| [growth of lignocellulolytic microorganisms on activated sludge: potential application]. | a culture medium containing activated sludge and sawdust supports the growth of numerous lignocellulolytic microorganisms. one of them, phanerochaete chrysosporium could be used in a composite feed for ruminants. | 1986 | 3689589 |
| oxidation of benzo(a)pyrene by extracellular ligninases of phanerochaete chrysosporium. veratryl alcohol and stability of ligninase. | benzo(a)pyrene was oxidized with crude and purified extracellular ligninase preparations from phanerochaete chrysosporium. both the crude enzyme and the purified fractions oxidized the substrate to three organic soluble products, namely benzo(a)pyrene 1,6-, 3,6-, and 6,12-quinones. these findings support the recent proposition that lignin-degrading enzymes are peroxidases, mediating oxidation of aromatic compounds via aryl cation radicals. the ligninase which was unstable in the presence of hydr ... | 1986 | 3700421 |
| fungi on the hair of large mammals in egypt. | the frequency of occurrence of fungi in 260 hair samples of dog, donkey and cow collected from veterinary medicine hospital of assiut university was determined. twenty-three species which belong to fifteen genera were collected. in low frequency, several dermatophytes (chrysosporium, microsporum, arthroderma and trichophyton) were found as well as some other fungal species pathogenic to man and animals (aspergillus fumigatus, stachybotrys chartarum, scopulariopsis brevicaulis and cephalosporium ... | 1986 | 3713793 |
| metabolites from a chrysosporium species. | | 1971 | 5168359 |
| enzymatic determination of veratryl alcohol. | a rapid and sensitive method was developed for the measurement of veratryl alcohol--a secondary metabolite of some lignin degrading fungi. the method is based on the enzymatic oxidation of veratryl alcohol to veratraldehyde by the ligninase of phanerochaete chrysosporium. the purified enzymes oxidized veratryl alcohol completely to veratraldehyde (75%) and some unidentified products. the enzymatic method was applied to measure veratryl alcohol in the culture filtrates of chrysosporium pruinosum ... | 1986 | 3717546 |
| isolation of keratinophilic fungi from floors in roman kindergarten and secondary schools. | we examined the dust collected from the floors of forty classrooms, twenty in kindergarten schools (children aged 2-5) and twenty in secondary schools (students aged 11-14) in order to determine the diffusion of keratinophilic fungi in respect to such different factors as human presence and children's age. in the kindergarten schools 268 colonies of keratinophilic fungi were isolated: 50 were microsporum, 6 trichophyton and 212 chrysosporium species. members of the chrysosporium genus were found ... | 1986 | 3724835 |
| [a nosological entity as yet unknown in romania: adiaspiromycosis]. | | 1987 | 3326141 |
| studies on compound i formation of the lignin peroxidase from phanerochaete chrysosporium. | ligninase, isolated from the wood-destroying fungus phanerochaete chrysosporium, catalyzes the oxidation of lignin and lignin-related compounds. ligninase reacts with h2o2 to form the classical peroxidase intermediates compounds i and ii. we have determined the activation energy of ligninase compound i formation to be 5.9 kcal/mol. the effect of ph and ionic strength on the rate of ligninase compound i formation was studied. in contrast to all other peroxidases, no ph effect was observed. this i ... | 1988 | 3335539 |
| [dermatophyte flora of the soil of asia minor and rhodes. isolation of trichophyton quinckeanum, trichophyton terrestre, chrysosporium keratinophilum and chrysosporium inducum]. | | 1968 | 5761166 |