| preliminary studies on the development of a microbiological treatment for polychlorinated biphenyls. | a series of experiments comprising microbiological testing, surfactant screening and microcosm systems are described for the preliminary development of a treatment for polychlorinated biphenyls (pcbs). microbial isolates from contaminated soil and sludge and culture collections, were screened for degradation of a defined mixture of pcb congeners in liquid culture. initially, one group of bacteria substantially degraded the dichloro-to pentachlorobiphenyls except where chlorinated at the 4,4' pos ... | 1990 | 21318507 |
| insight into functional diversity of cytochrome p450 in the white-rot basidiomycete phanerochaete chrysosporium: involvement of versatile monooxygenase. | to elucidate functional diversity of cytochrome p450 monooxygenases from the white-rot basidiomycete phanerochaete chrysosporium (pccyps), we conducted a comprehensive functional screening using a wide variety of compounds. a functionomic survey resulted in characterization of novel pccyp functions and discovery of versatile pccyps that exhibit broad substrate profiles. these results suggested that multifunctional properties of the versatile pccyps would play crucial roles in diversification of ... | 2011 | 21362401 |
| crystallographic, kinetic, and spectroscopic study of the first ligninolytic peroxidase presenting a catalytic tyrosine. | trametes cervina lignin peroxidase (lip) is a unique enzyme lacking the catalytic tryptophan strictly conserved in all other lips and versatile peroxidases (more than 30 sequences available). recombinant t. cervina lip and site-directed variants were investigated by crystallographic, kinetic, and spectroscopic techniques. the crystal structure shows three substrate oxidation site candidates involving his-170, asp-146, and tyr-181. steady-state kinetics for oxidation of veratryl alcohol (the typi ... | 2011 | 21367853 |
| isolation and characterization of a novel pyrene-degrading bacillus vallismortis strain jy3a. | the pahs-degrading bacterium strain jy3a was newly isolated from the polluted soil in the jinan oil refinery factory, shandong province of china. the isolate was identified as bacillus vallismortis with respect to its 16s rdna sequence, dna-dna relatedness and fatty acid profiles, as well as various physiological characteristics. the strain was gram-positive, motile, endospore forming, aerobic, oxidase and catalase-positive. the cells were 0.8-1.0µm wide and 2.0-2.5µm long, single or in pairs an ... | 2011 | 21371739 |
| determination of a catalytic tyrosine in trametes cervina lignin peroxidase with chemical modification techniques. | trametes cervina lignin peroxidase (lip) lacks a catalytic tryptophan strictly conserved in other lip and versatile peroxidases. it contains tyrosine(181) at the potential catalytic site. this protein and the well-characterized phanerochaete chrysosporium lip with the catalytic tryptophan(171) have been chemically modified: the tryptophan-specific modification with n-bromosuccinimide sufficiently disrupted oxidation of veratryl alcohol by p. chrysosporium lip, whereas the activity of t. cervina ... | 2011 | 21373922 |
| cytochrome b5 reductase-cytochrome b5 as an active p450 redox enzyme system in phanerochaete chrysosporium: atypical properties and in vivo evidence of electron transfer capability to cyp63a2. | two central redox enzyme systems exist to reduce eukaryotic p450 enzymes, the p450 oxidoreductase (por) and the cyt b5 reductase-cyt b5. in fungi, limited information is available for the cyt b(5) reductase-cyt b(5) system. here we characterized the kinetic mechanism of (cyt b5r)-cyt b5 redox system from the model white-rot fungus phanerochaete chrysosporium (pc) and made a quantitative comparison to the por system. we determined that pc-cyt b5r followed a "ping-pong" mechanism and could directl ... | 2011 | 21376009 |
| characterization of proteome alterations in phanerochaete chrysosporium in response to lead exposure. | | 2011 | 21388532 |
| heterologous expression of endo-1,4-beta-xylanasec from phanerochaete chrysosporium in pichia pastoris. | the cdna of endo-1,4-+¦-xylanasec, isolated from phanerochaete chrysosporium, was expressed in pichia pastoris, under the control of the alcohol oxidase i promoter. using either the intrinsic leader peptide of xylanasec or the +¦-factor signal peptide of saccharomyces cerevisiae, xylanasec is efficiently secreted into the medium, at a maximum concentration of 2500 u-àl(-1). | 2011 | 21393056 |
| microbial mannosidation of bioactive chlorogentisyl alcohol by the marine-derived fungus chrysosporium synchronum. | the biological transformation of the biologically active chlorogentisyl alcohol (1), isolated from the marine-derived fungus aspergillus sp., was studied. preparative-scale fermentation of chlorogentisyl alcohol with marine-derived fungus chrysosporium synchronum resulted in the isolation of a new glycosidic metabolite, 1-o-(a-d-mannopyranosyl)chlorogentisyl alcohol (2). the stereostructure of the new metabolite obtained was assigned on the basis of detailed spectroscopic data analyses, chemical ... | 2011 | 21467683 |
| cloning, expression and purification of the anion exchanger 1 homologue from the basidiomycete phanerochaete chrysosporium. | anion exchangers are membrane proteins that have been identified in a wide variety of species, where they transport cl(-) and hco3- across the cell membrane. in this study, we cloned an anion-exchange protein from the genome of the basidiomycete phanerochaete chrysosporium (pcaep). pcaep is a 618-amino acid protein that is homologous to the human anion exchanger (ae1) with 22.9% identity and 40.3% similarity. pcaep was overexpressed by introducing the pcaep gene into the genome of pichia pastori ... | 2011 | 21515379 |
| increasing manganese peroxidase productivity of phanerochaete chrysosporium by optimizing carbon sources and supplementing small molecules. | aims: to overproduce manganese peroxidase (mnp) using phanerochaete chrysosporium. effects of carbon sources, agricultural by-products and small molecules were tested to enhance the mnp productivity. methods and results: various carbon sources and agricultural by-products were compared as the basal medium. a mnp activity of 4·7 ± 0·31 u ml(-1) was obtained using mannose as a carbon source. the enzyme productivity further reached 7·36 ± 0·05 and 8·77 ± 0·23 u ml(-1) when the mannose medium was ... | 2011 | 21535047 |
| gene expression of wood decay fungi postia placenta and phanerochaete chrysosporium is significantly altered by plant species. | identification of specific genes and enzymes involved in conversion of lignocellulosics from an expanding number of potential feedstocks is of growing interest to bioenergy process development. basidiomycetous wood decay fungi phanerochaete chrysosporium and postia placenta are promising in this regard as they are able to utilize a wide range of simple and complex carbon compounds. however, systematic comparative studies with different woody substrates have not been reported. addressing this iss ... | 2011 | 21551287 |
| mycological survey of activated sludge in mbrs. | the objective of this research was to conduct a survey of fungi in activated sludge plants with membrane bioreactors (mbrs). thirty-six samples of both aerobic and anoxic activated sludge were taken from two plants with mbrs treating domestic wastewater. over a period of 8 months, two samples from each plant were taken per month. the samples were prepared for count and identification of fungi. the obtained data show that 61 species belonging to 30 genera were identified from activated sludge sam ... | 2011 | 21554420 |
| simultaneous cadmium removal and 2,4-dichlorophenol degradation from aqueous solutions by phanerochaete chrysosporium. | phanerochaete chrysosporium has been recognised as an effective bioremediation agent due to its unique degradation to xenobiotic and biosorption ability to heavy metals. however, few studies have focused on the simultaneous removal of heavy metals and organic pollutants. the aim of this work was to study the feasibility of simultaneous cadmium removal and 2,4-dichlorophenol (2,4-dcp) degradation in p. chrysosporium liquid cultures. the removal efficiencies were ph dependent and the maximum remov ... | 2011 | 21556917 |
| effects of xylan and starch on secretome of the basidiomycete phanerochaete chrysosporium grown on cellulose. | lignocellulosic biomass contains cellulose and xylan as major structural components, and starch as a storage polysaccharide. in the present study, we have used comparative secretomic analysis to examine the effects of xylan and starch on the expression level of proteins secreted by the basidiomycete phanerochaete chrysosporium grown on cellulose,. forty-seven spots of extracellular proteins expressed by p. chrysosporium separated by two-dimensional electrophoresis were identified by liquid chrom ... | 2011 | 21569082 |
| [breeding and characterization of laccase-producing phanerochaete chrysosporium mutant resistant to nutritional repression]. | to screen phanerochaete chrysosporium mutants resisting nutritional repression and to characterize laccase produced by the mutants. | 2011 | 21604549 |
| alternatives to trichoderma reesei in biofuel production. | mutant strains of trichoderma reesei are considered indisputable champions in cellulase production among biomass-degrading fungi. so, it is not surprising that most r&d projects on bioethanol production from lignocellulosics have been based on using t. reesei cellulases. the present review focuses on whether any serious alternatives to t. reesei enzymes in cellulose hydrolysis exist. although not widely accepted, more and more data have been accumulated that demonstrate that fungi belonging to t ... | 2011 | 21612834 |
| isolation, optimization, and partial purification of amylase from chrysosporium asperatum by submerged fermentation. | a potent fungus for amylase production, chrysosporium asperatum, was isolated from among 30 different cultures obtained from wood samples collected in the junagadh forest, india. all of the isolated cultures were screened for their ability to produce amylase by submerged fermentation. among the selected cultures, c. asperatum (class euascomycetes; onygenales; onygenaceae) gave maximum amylase production. in all of the different media tested, potato starch was found to be a good substrate for pro ... | 2011 | 21617343 |
| [extracellular polymeric substances (eps) of white-rot fungus and their effects on pb2+ adsorption by biomass]. | the extracellular polymeric substances (eps) of p. chrysosporium and their effects on pb2+ biosorption were studied. the product, composition of eps and the effects on pb2+ biosorption capacity were investigated in lab via flask experiments. the surface changes of mycelium before and after eps extraction, before and after pb2+ adsorption were researched by environment scanning electron microscope with energy-dispersive x-ray analysis (esem-edx). results showed that at 113 h, the maximum yield of ... | 2011 | 21634177 |
| efficacy of fungus mediated silver and gold nanoparticles against aedes aegypti larvae. | chrysosporium tropicum is a pathogenic fungus. it is known to be an effective mosquito control agent. in the present study, we have synthesized the silver and gold nanoparticles using c. tropicum. these nanoparticles have been characterized through microscan reader, x-ray diffractometer, transmission electron microscopy, and further confirmed by scanning electron microscopy. the characterization study confirmed the spherical shape and size (2-15 and 20-50 nm) of gold and silver nanoparticles. th ... | 2011 | 21647674 |
| simultaneous saccharification and fermentation of ground corn stover for the production of fuel ethanol using phanerochaete chrysosporium, gloeophyllum trabeum, saccharomyces cerevisiae, and escherichia coli k011. | enzymatic saccharification of corn stover using phanerochaete chrysosporium and gloeophyllum trabeum and subsequent fermentation of the saccharification products to ethanol by saccharomyces cerevisiae and escherichia coli k011 were achieved. prior to simultaneous saccharification and fermentation (ssf) for ethanol production, solid-state fermentation was performed for four days on ground corn stover using either p. chrysosporium or g. trabeum to induce in situ cellulase production. during ssf wi ... | 2011 | 21791956 |
| understanding ligninase-mediated reactions of endocrine disrupting chemicals in water: reaction rates and quantitative structure-activity relationships. | we have verified in our previous work that lignin peroxidase (lip) mediates effective removal of selected natural and synthetic estrogens. the efficiency of these reactions was greatly enhanced in the presence of veratryl alcohol (va), a chemical that is produced along with lip by certain white rot fungi, for example, phanerochaete chrysosporium. in this study, we systematically evaluated the kinetic behaviors of lip-mediated reactions for six endocrine disrupting compounds (edcs), that is, ster ... | 2011 | 21702433 |
| biotransformation of three pharmaceutical active compounds by the fungus phanerochaete chrysosporium in a fed batch stirred reactor under air and oxygen supply. | white-rot fungi are a group of microorganisms capable of degrading xenobiotic compounds, such as polycyclic aromatic hydrocarbons or synthetic dyes, by means of the action of extracellular oxidative enzymes secreted during secondary metabolism. in this study, the transformation of three anti-inflammatory drugs: diclofenac, ibuprofen and naproxen were carried out by pellets of phanerochaete chrysosporium in fed-batch bioreactors operating under continuous air supply or periodic pulsation of oxyge ... | 2011 | 21695453 |
| oxalate production at different initial pb(2+) concentrations and the influence of oxalate during solid-state fermentation of straw with phanerochaete chrysosporium. | the production of oxalate at different initial pb(2+) concentrations during solid-state fermentation of straw with phanerochaete chrysosporium was investigated. it was found that the maximal peak value of oxalate concentration (22.84mm) was detected at the initial pb(2+) concentration of 200mgkg(-1) dry straw, while the minimum (15.89mm) at the concentration of 600mgpb(2+)kg(-1) dry straw, and at moderate concentration of pb(2+) the capability of oxalic acid secretion was enhanced. in addition, ... | 2011 | 21700451 |
| Functional diversification of fungal glutathione transferases from the ure2p class. | The glutathione-S-transferase (GST) proteins represent an extended family involved in detoxification processes. They are divided into various classes with high diversity in various organisms. The Ure2p class is especially expanded in saprophytic fungi compared to other fungi. This class is subdivided into two subclasses named Ure2pA and Ure2pB, which have rapidly diversified among fungal phyla. We have focused our analysis on Basidiomycetes and used Phanerochaete chrysosporium as a model to corr ... | 2011 | 22164343 |
| Molecular identification and functional characterization of cytochrome P450 monooxygenases from the brown-rot basidiomycete Postia placenta. | We explored the molecular diversity and functional capabilities of cytochrome P450 monooxygenases (P450s) from the brown-rot basidiomycete Postia placenta. Using bioinformatic and experimental data, we found 250 genes of P450s in the whole genome, including 60 putative allelic variants. Phylogenetic analysis revealed the presence of 42 families, including 18 novel families. Comparative phylogenetic analysis of P450s from P. placenta and the white-rot basidiomycete Phanerochaete chrysosporium sug ... | 2011 | 21938516 |
| characterization of a recombinant aryl β-glucosidase from neosartorya fischeri nrrl181. | an isolated gene from neosartorya fischeri nrrl181 encoding a β-glucosidase (bgl) was cloned, and its nucleotide sequence was determined. dna sequence analysis revealed an open reading frame of 1,467 bp, capable of encoding a polypeptide of 488 amino acid residues. the gene was over-expressed in escherichia coli, and the protein was purified using nickel-nitrilotriacetic acid chromatography. the purified recombinant bgl showed a high level of catalytic activity, with v (max) ... | 2011 | 22042231 |
| phanerochaete chrysosporium ibl-03 secretes high titers of manganese peroxidase during decolorization of drimarine blue k2rl textile dye. | a novel indigenous strain, phanerochaete chrysosporium ibl-03, with high manganese peroxidase (mnp) activities was used for decolorization of a reactive textile dye, drimarine blue k2r, which is used extensively in textile units of pakistan. the initial experiment was run for seven days with 0.01% (w/v) dye solution prepared in kirk's basal nutrient medium. samples were removed after every 24 h and the extent of dye decolorization was determined at lambda(max) of the dye. the study revealed that ... | 2011 | 21970166 |
| phylogeny and intraspecific variation of the extreme xerophile, xeromyces bisporus. | the filamentous ascomycete xeromyces bisporus is an extreme xerophile able to grow down to a water activity of 0.62. we have inferred the phylogenetic position of xeromyces in relation to other xerophilic and xerotolerant fungi in the order eurotiales. using nrdna and betatubulin sequences, we show that it is more closely related to the xerophilic foodborne species of the genus chrysosporium, than to the genus monascus. the taxonomy of x. bisporus and monascus is discussed. based on physiologica ... | 2011 | 22036289 |
| entrapment of mycelial fragments in calcium alginate: a general technique for the use of immobilized filamentous fungi in biocatalysis. | transformation reactions on 3β,17β-dihydroxyandrost-5-ene using free fungal cells were compared with those carried out by macerated mycelia, immobilized in calcium alginate beads. six fungi were utilized in this study, namely rhizopus oryzae atcc 11145, mucor plumbeus atcc 4740, cunninghamella echinulata var. elegans atcc 8688a, aspergillus niger atcc 9142, phanerochaete chrysosporium atcc 24725 and whetzelinia sclerotiorum atcc 18687. the results show, for the first time, that encapsulated myce ... | 2012 | 22064215 |
| a novel combined thermometric and amperometric biosensor for lactose determination based on immobilised cellobiose dehydrogenase. | a novel method for lactose determination in milk is proposed. it is based on oxidation of lactose by cellobiose dehydrogenase (cdh) from the basidiomycete phanerochaete chrysosporium, immobilised in an enzyme reactor. the reactor was prepared by cross-linking cdh onto aminopropyl-silanised controlled pore glass (cpg) beads using glutaraldehyde. the combined biosensor worked in flow injection analysis (fia) mode and was developed for simultaneous monitoring of the thermometric signal associated w ... | 2012 | 22078845 |
| artificial construction and characterization of a fungal consortium that produces cellulolytic enzyme system with strong wheat straw saccharification. | a consortium-apct2 composed of 92% of trichoderma sp. t-1, 6.7% of p. chrysosporium and 1.3% of a. oryzae a-4 that produces cellulolytic enzyme system with strong wheat straw saccharification was constructed using taguchi design combined with variance analysis (anova). among 32 consortia constructed from 6 fungi using taguchi design, consortium 20 with the strongest composition was selected. the inhibitive fungal constituent in consortium 20 was subsequently removed according to the anova result ... | 2011 | 21924894 |
| the ferulic acid esterases of chrysosporium lucknowense c1: purification, characterization and their potential application in biorefinery. | three ferulic acid esterases from the filamentous fungus chrysosporium lucknowense c1 were purified and characterized. the enzymes were most active at neutral ph and temperatures up to 45 °c. all enzymes released ferulic acid and p-coumaric acid from a soluble corn fibre fraction. ferulic acid esterases faea1 and faea2 could also release complex dehydrodiferulic acids and dehydrotriferulic acids from corn fibre oligomers, but released only 20% of all ferulic acid present in sugar beet pectin oli ... | 2012 | 22133444 |
| quantitative proteomic analysis of lignocellulolytic enzymes by phanerochaete chrysosporium on different lignocellulosic biomass. | lignocellulosic biomass from agricultural crop residues and forest waste represents an abundant renewable resource for bioenergy and future biofuel. the current bottleneck of lignocellulosic biofuel production is the hydrolysis of biomass to sugar. to understand the enzymatic hydrolysis of complex biomasses, in this report, lignocellulolytic enzymes secretion by phanerochaete chrysosporium cultivated in different natural lignocellulosic biomass such as corn stover, hay, sawdust, sugarcane baggas ... | 2011 | 22146477 |
| characterization and mode of action of two acetyl xylan esterases from chrysosporium lucknowense c1 active towards acetylated xylans. | two novel acetyl xylan esterases, axe2 and axe3, from chrysosporium lucknowense (c1), belonging to the carbohydrate esterase families 5 and 1, respectively, were purified and biochemically characterized. axe2 and axe3 are able to hydrolyze acetyl groups both from simple acetylated xylo-oligosaccharides and complex non-soluble acetylglucuronoxylan. both enzymes performed optimally at ph 7.0 and 40°c. axe2 has a clear preference for acetylated xylo-oligosaccharides (acxos) with a high degree of su ... | 2011 | 22112517 |
| chrysosporium sp. infection in eastern massasauga rattlesnakes. | to the editor: during 2008, the ninth year of a long-term biologic monitoring program, 3 eastern massasauga rattlesnakes (sistrurus catenatus catenatus) with severe facial swelling and disfiguration died within 3 weeks after discovery near carlyle, illinois, usa. in spring 2010, a similar syndrome was diagnosed in a fourth massasauga; this snake continues to be treated with thermal and nutritional support and antifungal therapy. a keratinophilic fungal infection caused by chrysosporium sp. was d ... | 2011 | 22172594 |
| composition and antifungal activities of the leaf essential oil of neolitsea parvigemma from taiwan. | the hydrodistillated leaf essential oil of neolitsea parvigemma was analyzed to determine its composition and yield. sixty-two compounds were identified, the main components being beta-caryophyllene (14.2%), beta-eudesmol (12.9%), alpha-cadinol (10.2%) and tau-cadinol (8.8%). oxygenated sesquiterpenes (48.9%) and sesquiterpene hydrocarbons (48.8%) were the predominant groups of compounds. the antifungal indexes of the leaf oil against the 7 fungi, aspergillus clavatus, a. niger, chaetomium globo ... | 2011 | 21941915 |
| the putative endoglucanase pcgh61d from phanerochaete chrysosporium is a metal-dependent oxidative enzyme that cleaves cellulose. | many fungi growing on plant biomass produce proteins currently classified as glycoside hydrolase family 61 (gh61), some of which are known to act synergistically with cellulases. in this study we show that pcgh61d, the gene product of an open reading frame in the genome of phanerochaete chrysosporium, is an enzyme that cleaves cellulose using a metal-dependent oxidative mechanism that leads to generation of aldonic acids. the activity of this enzyme and its beneficial effect on the efficiency of ... | 2011 | 22132148 |
| itraq-based quantitative secretome analysis of phanerochaete chrysosporium. | the basidiomycete fungi such as phanerochaete chrysosporium secrete large amount of hydrolytic and oxidative enzymes and degrade lignocellulosic biomass. the lignin depolymerizing proteins were extensively studied, but cellulose, hemicellulose and pectin hydrolyzing enzymes were poorly explored. in this study p. chrysosporium was grown in cellulose, lignin and mixture of cellulose and lignin, and secretory proteins were quantified by isobaric tag for relative and absolute quantitation (itraq)-ba ... | 2011 | 21945728 |
| deep fungal dermatitis caused by the chrysosporium anamorph of nannizziopsis vriesii in captive coastal bearded dragons (pogona barbata). | deep fungal dermatitis caused by the chrysosporium anamorph of nannizziopsis vriesii (canv) was diagnosed in a group of coastal bearded dragons (pogona barbata). the outbreak extended over a 6-month period, with four of six lizards from the same zoological outdoor enclosure succumbing to infection. a fifth case of dermatomycosis was identified in a pet lizard originally sourced from the wild. diagnosis of infection with the canv was based on similar clinical signs and histopathology in all anima ... | 2011 | 22103953 |
| A Fungal P450 (CYP5136A3) Capable of Oxidizing Polycyclic Aromatic Hydrocarbons and Endocrine Disrupting Alkylphenols: Role of Trp and Leu. | The model white rot fungus Phanerochaete chrysosporium, which is known for its versatile pollutant-biodegradation ability, possesses an extraordinarily large repertoire of P450 monooxygenases in its genome. However, the majority of these P450s have hitherto unknown function. Our initial studies using a genome-wide gene induction strategy revealed multiple P450s responsive to individual classes of xenobiotics. Here we report functional characterization of a cytochrome P450 monooxygenase, CYP5136A ... | 2011 | 22164262 |
| Heterologous expression and mechanistic investigation of a fungal cytochrome P450 (CYP5150A2): Involvement of alternative redox partners. | A fungal cytochrome P450 monooxygenase (CYP5150A2) from the white-rot basidiomycete Phanerochaete chrysosporium was heterologously expressed in Escherichia coli and purified as an active form. The purified CYP5150A2 was capable of hydroxylating 4-propylbenzoic acid (PBA) with NADPH-dependent cytochrome P450 oxidoreductase (CPR) as the single redox partner; the reaction efficiency was improved by the addition of electron transfer protein cytochrome b5 (Cyt-b5). Furthermore, CYP5150A2 exhibited su ... | 2011 | 22206618 |
| Flavin-containing monooxygenases from Phanerochaete chrysosporium responsible for fungal metabolism of phenolic compounds. | We investigated the cellular responses of the white-rot basidiomycete Phanerochaete chrysosporium against vanillin. Based upon a proteomic survey, it was demonstrated that two flavin-containing monooxygenases (PcFMO1 and PcFMO2) are translationally up-regulated in response to exogenous addition of vanillin. To elucidate their catalytic functions, we cloned cDNAs and heterologously expressed them in Escherichia coli. The recombinant PcFMO1 showed catalytic activities against monocyclic phenols su ... | 2011 | 22102096 |
| Biodegradation pathway and detoxification of the diazo dye Reactive Black 5 by Phanerochaete chrysosporium. | The in vivo biodegradation of the diazo dye Reactive Black 5 (RB5) by Phanerochaete chrysosporium immobilised on cubes of nylon sponge and on sunflower-seed shells (SS) in laboratory-scale bioreactors was investigated. The SS cultivation led to the best results with a decolouration percentage of 90.3% in 72 h for an initial RB5 concentration of 100 mg/L. It was found that the addition of 0.4 mM veratryl alcohol (VA) into the medium considerably increased the decolouration rate in SS cultivation. ... | 2011 | 21955876 |
| chrysosporium lucknowense c1 arabinofuranosidases are selective in releasing arabinose from either single or double substituted xylose residues in arabinoxylans. | two novel arabinofuranosidases, abn7 and abf3 from chrysosporium lucknowense (c1), belonging to the glycoside hydrolase family 43 and 51 were purified and characterized. abn7 is exclusively able to hydrolyze arabinofuranosyl residues at position o-3 of double substituted xylosyl residues in arabinoxylan-derived oligosaccharides, an activity rarely found thus far. abf3 is able to release arabinose from position o-2 or o-3 of single substituted xyloses. both enzymes performed optimal at ph 5.0 and ... | 2011 | 22112956 |
| reductive dehalogenation of aliphatic halocarbons by lignin peroxidase of phanerochaete chrysosporium. | | 1995 | 22200281 |
| High throughput screening of fungal endoglucanase activity in Escherichia coli. | Cellulase enzymes (endoglucanases, cellobiohydrolases, and ß-glucosidases) hydrolyze cellulose into component sugars, which in turn can be converted into fuel alcohols. The potential for enzymatic hydrolysis of cellulosic biomass to provide renewable energy has intensified efforts to engineer cellulases for economical fuel production. Of particular interest are fungal cellulases, which are already being used industrially for foods and textiles processing. Identifying active variants among a libr ... | 2011 | 21860379 |
| A comparative characterization of indigenous keratinase enzymes from district Khairpur, Sindh, Pakistan. | To isolate and characterize keratinolytic fungi and bacteria from indigenous soils, a total of 80 samples were collected from Ghari Mori District. Khairpur, and these organisms were isolated using standard microbiological technique. The isolated keratinolytic microorganisms comprised: Absidia sp., Chrysosporium asperatum, Chrysosporium keratinophilum, Entomophthora coronata, Bacillus subtilis and Staphylococcus aureus and their keratinolytic properties were distinguished from the production of k ... | 2012 | 22186312 |
| Effective Removal of Endocrine-Disrupting Compounds by Lignin Peroxidase from the White-Rot Fungus Phanerochaete sordida YK-624. | The removal of endocrine-disrupting compounds (EDCs) by lignin peroxidase from white-rot fungus Phanerochaete sordida YK-624 (YK-LiP1) was investigated. Five endocrine disruptors, p-t-octylphenol (OP), bisphenol A (BPA), estrone (E(1)), 17ß-estradiol (E(2)), and ethinylestradiol (EE(2)) were eliminated by YK-LiP1 more effectively than lignin peroxidase from P. chrysosporium (Pc-LiP), and OP and BPA were disappeared almost completely in the reaction mixture containing YK-LiP1 after a 24-h treatme ... | 2011 | 22203416 |
| Lignolytic Enzymes of a Mushroom Stereum ostrea Isolated from Wood Logs. | Production of lignolytic enzymes by the mushroom fungus Stereum ostrea in liquid medium under conditions of vegetative growth was examined for 10 days in comparison to the reference culture Phanerochaete chrysosporium. Though growth and secretion of extracellular protein by S. ostrea were comparable to those of P. chrysosporium, yields of laccase enzyme by S. ostrea were higher than laccase titres of P. chrysosporium by more than 2 folds on the peak production time interval (IVth day of incubati ... | 2011 | 21941632 |
| fungal diversity in cow, goat and ewe milk. | knowledge of fungal diversity in the environment is poor compared with bacterial biodiversity. in this study, we applied the denaturing high-performance liquid chromatography (d-hplc) technique, combined with the amplification of the its1 region from fungal rdna, for the rapid identification of major fungal species in 9 raw milk samples from cow, ewe and goat, collected at different periods of the year. a total of 27 fungal species were identified. yeast species belonged to candida, cryptococcus ... | 2011 | 21944758 |
| identification of cytochrome p450 monooxygenase genes from the white-rot fungus phlebia brevispora. | abstract: three cytochrome p450 monooxygenase (cyp) genes, designated pb-1, pb-2 and pb-3, were isolated from the white-rot fungus, phlebia brevispora, using reverse transcription pcr with degenerate primers constructed based on the consensus amino acid sequence of eukaryotic cyps in the o2-binding, meander and heme-binding regions. individual full-length cyp cdnas were cloned and sequenced, and the relative nucleotide sequence similarity of pb-1 (1788 bp), pb-2 (1881 bp) and pb-3 (1791 bp) was ... | 2012 | 22273259 |
| effects of phosphorus concentration on the growth and enzyme production of phanerochaete chrysosporium. | the effects of different phosphorus concentrations in culture media on the growth and enzyme production of phanerochaete chrysosporium was investigated at a glucose concentration of 10gl(-1). the results showed that the optimal kh(2)po(4) concentration was 2.0gl(-1). optimal phosphorus content not only supported robust growth of p. chrysosporium, but also helped produce higher yields of manganese-dependent peroxidase (mnp) (324.9ul(-1)). in addition, the results revealed that a relationship betw ... | 2012 | 22248801 |
| a case of adiaspiromycosis causing respiratory failure and a review of human infections due to the fungi emmonsia and chrysosporium. | we report a case of a 27 year-old male who presented with respiratory distress that required mechanical ventilation. transbronchial biopsy revealed adiaspores of the fungus emmonsia crescens within granulomata, a condition known as adiaspiromycosis. the patient received amphotericin products and corticosteroids, followed by itraconazole, and made a full recovery. emmonsia crescens is a saprobe with a wide distribution that is primarily a rodent pathogen. the clinical characteristics of the 20 ca ... | 2012 | 22259200 |