Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| comparative activities of six different fluoroquinolones against 9,682 clinical bacterial isolates from 20 european university hospitals participating in the european sentry surveillance programme. the sentry participants group. | the in-vitro activities of gatifloxacin, trovafloxacin, levofloxacin, sparfloxacin, ofloxacin, and ciprofloxacin were tested against 9,682 clinical bacterial isolates from 20 european university hospitals participating in the european sentry surveillance programme. gatifloxacin and trovafloxacin exhibited the highest activities against gram-positive cocci, while levofloxacin, ofloxacin, ciprofloxacin, and gatifloxacin were the most active against enterobacteriaceae. ciprofloxacin and levofloxaci ... | 1999 | 10493607 |
| characterization of the chromosomal aac(6')-iz gene of stenotrophomonas maltophilia. | the aac(6')-iz gene of stenotrophomonas maltophilia bm2690 encoding an aminoglycoside 6'-n-acetyltransferase was characterized. the gene was identified as a coding sequence of 462 bp corresponding to a protein with a calculated mass of 16,506 da, a value in good agreement with that of ca. 16,000 found by in vitro coupled transcription-translation. analysis of the deduced amino acid sequence indicated that the protein was a member of the major subfamily of aminoglycoside 6'-n-acetyltransferases. ... | 1999 | 10508008 |
| carbapenem derivatives as potential inhibitors of various beta-lactamases, including class b metallo-beta-lactamases. | a variety of 1beta-methylcarbapenem derivatives were screened to identify inhibitors of imp-1 metallo-beta-lactamase, a class b beta-lactamase, in an automated microassay system using nitrocefin as a substrate. the structure-inhibitory-activity relationship study revealed that three types of 1beta-methylcarbapenems having benzothienylthio, dithiocarbamate, or pyrrolidinylthio moieties at the c-2 position showed good inhibitory activity. among the compounds screened, j-110,441, having a benzothie ... | 1999 | 10508031 |
| fate of plasmid-bearing, luciferase marker gene tagged bacteria after feeding to the soil microarthropod onychiurus fimatus (collembola). | in order to study the potential impact of the soil microarthropod onychiurus fimatus (collembola) on the microbial community, we analysed the fate of luciferase marker gene tagged bacterial strains fed to young adult specimens in petri dish microcosm experiments. in faeces collected from o. fimatus, escherichia coli s17-1/prp4luc and sinorhizobium meliloti l33 were only detectable for 2 days after feeding whereas strain hr2/prp4luc, a close relative of stenotrophomonas maltophilia, isolated from ... | 1999 | 10508937 |
| faucet aerators: a source of patient colonization with stenotrophomonas maltophilia. | 1999 | 10511498 | |
| stenotrophomonas maltophilia: an increasing problem in patients with acute leukaemia. | stenotrophomonas maltophilia (previously pseudomonas maltophilia and xanthomonas maltophilia) is an increasing problem as an opportunistic pathogen in immunocompromised patients such as those with acute leukaemia. infection is difficult to treat and eradicate due to its inherent and quickly acquired resistance to many antibiotics. presentation with unusual cutaneous lesions is not uncommon and can present diagnostic difficulties. we present two cases which highlight the classical features of thi ... | 1999 | 10512179 |
| genotypic and phenotypic relationships between clinical and environmental isolates of stenotrophomonas maltophilia. | while the gram-negative bacterium stenotrophomonas maltophilia is used in biotechnology (e.g., for biological control of plant pathogens and for bioremediation), the number of s. maltophilia diseases in humans has dramatically increased in recent years. a total of 40 s. maltophilia isolates from clinical and environmental sources (plant associated and water) was investigated to determine the intraspecies diversity of the group and to determine whether or not the strains could be grouped based on ... | 1999 | 10523559 |
| changing epidemiology of infections in patients with neutropenia and cancer: emphasis on gram-positive and resistant bacteria. | over the past 3 decades, considerable changes have occurred in the types of bacteria causing infection in febrile patients with neutropenia and cancer. twenty years ago, gram-negative bacteria caused approximately 70% of bloodstream infections. as a probable consequence of long-dwelling intravascular devices, fluoroquinolone prophylaxis, and high-dose chemotherapy-induced mucositis, there has been a shift toward gram-positive coccal bacteremia. in most centers today, approximately 70% of bactere ... | 1999 | 10530434 |
| endotoxins and igg antibodies as indicators of occupational exposure to the microbial contaminants of metal-working fluids. | the aim of this study was to evaluate workers' exposure to microbes and bacterial endotoxins during the use of metal-working fluids (mwf). | 1999 | 10541909 |
| selective hydrolysis of nucleotides to nucleosides and free bases. | the kinetics of the hydrolysis of 2'-deoxyadenosine-5'-monophosphoric acid (damp), 2'-deoxycytidine-5'-monophosphoric acid (dcmp), 2'-deoxyguanosine-5'-monophosphoric acid (dgmp) and tymidine-5'-monophosphoric acid (dtmp) was studied in the presence of xanthomonas maltophilia [1]. the reaction products are nucleosides: 2'-deoxyadenosine (da), 2'-deoxycytidine (dc), 2'-deoxyguanosine (dg) and tymidine (dt), respectively, or the respective free bases. hydrolysis of dtmp and dgmp proceeded stepwise ... | 1999 | 10555263 |
| genomic diversity of the genus stenotrophomonas. | the clinical and environmental importance of stenotrophomonas bacteria requires thorough, molecular studies on their epidemiology and taxonomy. in order to obtain a complete genomic profile of this genus, over 100 stenotrophomonas maltophilia strains from various origins were investigated by aflp fingerprinting. a subset of these strains was analysed by dna hybridization and 16s rdna sequencing. in contrast to their high phenotypic homogeneity, the strains were found to be very heterogeneous gen ... | 1999 | 10555357 |
| [stenotrophomonas maltophilia endocarditis following mitral valve prosthesis implantation. report of a case]. | the authors report the first case of early postoperative endocarditis after mitral valvuloplasty due to stenotrophomonas maltophilia, a gram negative organism, in a 37 year old man with no special risk factors. pyrexia and mitral valve vegetations were the main features, and, in the absence of complications or of embolism, the patient was treated initially with triple antibiotherapy (ceftazidime, amikacine, ciprofloxacine). relapse two weeks after withdrawal of treatment due to two variants of s ... | 1999 | 10562908 |
| nosocomial infections due to stenotrophmonas maltophilia. | 1999 | 10563742 | |
| nosocomial pneumonia likely caused by stenotrophomonas maltophilia in two patients with polymyositis. | we report two cases of polymyositis (pm) complicated with nosocomial pneumonia probably caused by stenotrophomonas maltophilia, which was resistant to multiple antimicrobials. in the first case, the chest ct findings and high serum endotoxin level as well as sputum culture results were helpful for the proper diagnosis and the therapy was successful. however the second patient died of a lung abscess in spite of the intensive antibiotic therapy. when pm patients develop pneumonia unresponsive to v ... | 1999 | 10563756 |
| species-specific detection of hydrocarbon-utilizing bacteria. | rapid detection and quantitative assessment of specific microbial species in environmental samples is desirable for monitoring changes in ecosystems and for tracking natural or introduced microbial species during bioremediation of contaminated sites. in the interests of developing rapid tests for hydrocarbon-degrading bacteria, species-specific pcr primer sets have been developed for pseudomonas aeruginosa, stentrophomonas (xanthomonas) maltophilia, and serratia marsescens. highly variable regio ... | 1999 | 10579508 |
| outbreak of stenotrophomonas maltophilia bacteremia among patients undergoing bone marrow transplantation: association with faulty replacement of handwashing soap. | using molecular typing methods, we confirmed an outbreak of stenotrophomonas maltophilia among bone marrow transplant patients. the likely source was a healthcare worker who may have washed with moisturizer instead of soap between patients. hospital epidemiologists need to go beyond antibiograms when evaluating outbreaks and be vigilant about all aspects of hand washing. | 1999 | 10580627 |
| [cloning and expression of tyrosinase gene from pseudomonas maltophilia in e. coli]. | the enzyme tyrosinase, encoded by tyrosinase gene (mel), is responsible for melanin formation. in a shotgun cloning experiment, a sali-digested dna fragment coding for tyrosinase was cloned from pseudomonas maltophilia dna into plasmid vector (puc18) to generate the hybrid plasmid (pwsy). the recombinant plasmid imparted the ability of melanin synthesis to an e. coli host (hb101). the foreign dna fragment (0.7 kb) possessed no recognition sites for bamhi, hindiii, ecori or bcli. hybridization st ... | 1999 | 10589169 |
| evidence of nosocomial stenotrophomonas maltophilia cross-infection in a neonatology unit analyzed by three molecular typing methods. | to characterize the epidemiological relationships among stenotrophomonas maltophilia isolates in the neonatology unit of our institution over a 4-month period in which an increased number of isolates was observed. | 1999 | 10614605 |
| endotoxic activity of lipopolysaccharides isolated from emergent potential cystic fibrosis pathogens. | improved antimicrobial therapies against the classical spectrum of pathogenic bacteria which colonise the lungs of cystic fibrosis (cf) patients has resulted in improved life expectancy and quality of life. bacterial species that are resistant to a broad range of antibiotics including stenotrophomonas maltophilia and alcaligenes xylosoxidans have now emerged as potential new pathogens to fill the niche. at present, it is unclear from clinical data whether these microbes are commensal or pathogen ... | 2000 | 10617793 |
| outbreak of stenotrophomonas maltophilia bacteremia in allogenic bone marrow transplant patients: role of severe neutropenia and mucositis. | from march 1997 through november 1997, 8 allogenic bone marrow transplant (bmt) patients developed stenotrophomonas maltophilia bacteremia on the hematology service at ucla medical center (los angeles). five of these patients had undergone transplantation during the same hospitalization that s. maltophilia bacteremia was detected (case patients). compared with 7 concurrently hospitalized allogenic bmt patients (control patients), the 5 case patients were more likely to have been hospitalized in ... | 2000 | 10619754 |
| levofloxacin in vitro activity and time-kill evaluation of stenotrophomonas maltophilia clinical isolates. | the in vitro activity of levofloxacin and eight other antimicrobial agents against 60 clinical isolates of stenotrophomonas maltophilia was determined by an agar dilution method using 10(4) and 10(6) cfu/spot inocula. at the lower inoculum, 85.0% of the isolates were susceptible to levofloxacin but only 58.3% were susceptible to ofloxacin; at the higher inoculum, 78.3% were susceptible to levofloxacin and 36.7% to ofloxacin. in time-kill studies, levofloxacin exerted bactericidal activity within ... | 2000 | 10629022 |
| multiple antibiotic resistance in stenotrophomonas maltophilia: involvement of a multidrug efflux system. | clinical strains of stenotrophomonas maltophilia are often highly resistant to multiple antibiotics, although the mechanisms of resistance are generally poorly understood. multidrug resistant (mdr) strains were readily selected by plating a sensitive reference strain of the organism individually onto a variety of antibiotics, including tetracycline, chloramphenicol, ciprofloxacin, and norfloxacin. tetracycline-selected mdr strains typically showed cross-resistance to erythromycin and fluoroquino ... | 2000 | 10639352 |
| association of qace and qacedelta1 with multiple resistance to antibiotics and antiseptics in clinical isolates of gram-negative bacteria. | clinical isolates of enterobacter cloacae, citrobacter freundii, pseudomonas aeruginosa, and stenotrophomonas maltophilia were tested for resistance to antibiotics and to the antiseptics benzalkonium chloride and cetyltrimethylammonium bromide. furthermore, they were examined for the presence of the resistance genes qace and qacedelta1. qacedelta1 was detected by pcr in 10% of all (n=103) and in 81% of multiply antibiotic-resistant strains (n=15). qace was found in only one of 37 p. aeruginosa s ... | 2000 | 10650208 |
| specific and rapid detection by fluorescent in situ hybridization of bacteria in clinical samples obtained from cystic fibrosis patients. | we report on the rapid and specific detection of bacteria commonly isolated from clinical specimens from cystic fibrosis (cf) patients by fluorescent in situ hybridization (fish). on the basis of comparative sequence analysis, we designed oligonucleotide probes complementary to species-specific 16s rrna regions of these microorganisms and demonstrated the specificities of the probes by hybridization of different remotely related as well as closely related reference strains. furthermore, in a pil ... | 2000 | 10655391 |
| biochemical sequence analyses of ges-1, a novel class a extended-spectrum beta-lactamase, and the class 1 integron in52 from klebsiella pneumoniae. | klebsiella pneumoniae ori-1 was isolated in 1998 in france from a rectal swab of a 1-month-old girl who was previously hospitalized in cayenne hospital, cayenne, french guiana. this strain harbored a ca. 140-kb nontransferable plasmid, ptk1, that conferred an extended-spectrum cephalosporin resistance profile antagonized by the addition of clavulanic acid, tazobactam, or imipenem. the gene for ges-1 (guiana extended-spectrum beta-lactamase) was cloned, and its protein was expressed in escherichi ... | 2000 | 10681329 |
| in vitro activity of minocycline against respiratory pathogens from patients with cystic fibrosis. | our objective was to determine the in vitro activity of minocycline against isolates of burkholderia cepacia (bc), stenotrophomonas maltophilia (sm), and pseudomonas aeruginosa (pa) cultured from the respiratory tract of patients with cystic fibrosis (cf). cultures of bc, sm, and pa were isolated in a hospital bacteriology laboratory from the sputum or oropharyngeal cultures obtained from patients attending a cystic fibrosis center, and were prospectively tested for in vitro sensitivity to minoc ... | 2000 | 10686042 |
| comparative in vitro activity of quinolones against stenotrophomonas maltophilia. | the susceptibility of 109 stenotrophomonas maltophilia isolates, all characterized by pulsed-field gel electrophoresis, to nine quinolones was studied. grepafloxacin, trovafloxacin, and moxifloxacin displayed similar intrinsic activities (mic90, 0.5 microg/ml), which were lower than those of ofloxacin and ciprofloxacin (mic90, 4 microg/ml), norfloxacin (mic90, 64 microg/ml), and nalidixic acid (mic90, 32 microg/ml). nalidixic acid was generally one- to twofold dilutions more active than norfloxa ... | 1999 | 10691206 |
| degradation and mineralization of high-molecular-weight polycyclic aromatic hydrocarbons by defined fungal-bacterial cocultures. | this study investigated the biodegradation of high-molecular-weight polycyclic aromatic hydrocarbons (pahs) in liquid media and soil by bacteria (stenotrophomonas maltophilia vun 10,010 and bacterial consortium vun 10,009) and a fungus (penicillium janthinellum vuo 10, 201) that were isolated from separate creosote- and manufactured-gas plant-contaminated soils. the bacteria could use pyrene as their sole carbon and energy source in a basal salts medium (bsm) and mineralized significant amounts ... | 2000 | 10698765 |
| failure of an automated blood culture system to detect nonfermentative gram-negative bacteria. | during a 1-year study we observed that both aerobic and anaerobic blood culture bottles from patients were negative by the bact/alert system during a 7-day incubation period. however, upon subcultivation of negative bottles, growth of pseudomonas aeruginosa was detectable. in an attempt to explain this observation, aerobic bact/alert fan bottles were seeded with a defined inoculum (0.5 mcfarland standard; 1 ml) of escherichia coli, klebsiella pneumoniae, serratia marcescens, p. aeruginosa, steno ... | 2000 | 10698992 |
| in vitro antibacterial activity and mechanism of action of j-111,225, a novel 1beta-methylcarbapenem, against transferable imp-1 metallo-beta-lactamase producers. | imp-1 beta-lactamase, a class b zinc metallo-enzyme encoded by the transferable bla(imp) gene, is known to confer high-level resistance to carbapenems as well as to penicillins and cephalosporins. j-111, 225 is a novel 1beta-methylcarbapenem with a structurally unique side chain comprising a trans-3,5-disubstituted pyrrolidinylthio moiety at the c2 position. it inhibited 17 serratia marcescens and two pseudomonas aeruginosa imp-1-producing clinical isolates at a concentration of 32 mg/l (range 4 ... | 2000 | 10702544 |
| comparative activity of new quinolones against 326 clinical isolates of stenotrophomonas maltophilia. | stenotrophomonas maltophilia is an important emerging pathogen causing a variety of infections in severely ill patients. this microorganism is inherently resistant to many antibiotics, and only a few therapeutic options are available. the principal aim of this study was to assess the in vitro activity of new quinolones against this pathogen. three hundred and twenty-six single clinical isolates were tested in this study. the mic(90) was 16 mg/l for ciprofloxacin, 8 mg/l for levofloxacin and gati ... | 2000 | 10702558 |
| characterization of vim-2, a carbapenem-hydrolyzing metallo-beta-lactamase and its plasmid- and integron-borne gene from a pseudomonas aeruginosa clinical isolate in france. | pseudomonas aeruginosa col-1 was identified in a blood culture of a 39-year-old-woman treated with imipenem in marseilles, france, in 1996. this strain was resistant to beta-lactams, including ureidopenicillins, ticarcillin-clavulanic acid, cefepime, ceftazidime, imipenem, and meropenem, but remained susceptible to the monobactam aztreonam. the carbapenem-hydrolyzing beta-lactamase gene of p. aeruginosa col-1 was cloned, sequenced, and expressed in escherichia coli dh10b. the deduced 266-amino-a ... | 2000 | 10722487 |
| cloning and overexpression of a tyrosinase gene mel from pseudomonas maltophila. | the tyrosinase gene (mel), which is responsible for melanin formation, was isolated by shotgun cloning of sali fragments of pseudomonas maltophila dna. a 0.7-kb sali fragment in the recombinant plasmid pwsy8 imparted the ability to synthesize melanin to an escherichia coli host hb101. the nucleotide sequence of this dna fragment revealed an open reading frame of 504 bp, encoding a protein of 169 amino acids. the fragment containing the mel gene was then cloned into an expression plasmid ppas1 un ... | 2000 | 10731602 |
| stenotrophomonas maltophilia and lawn mower injuries in children. | 2000 | 10744300 | |
| antimicrobial activity and spectrum of the new glycylcycline, gar-936 tested against 1,203 recent clinical bacterial isolates. | the in vitro activity of gar-936, a new semisynthetic glycylcycline, was evaluated in comparison with two tetracyclines and several other antimicrobial agents. a total of 1,203 recent clinical isolates were tested by reference broth or agar dilution methods. among the members of the family enterobacteriaceae, gar-936 was generally two- to four-fold more active than minocycline, and two- to 16-fold more active than tetracycline. all enteric bacilli mic90 results were < or = 4 microg/ml; the excep ... | 2000 | 10744364 |
| in vitro antibacterial spectrum of a new broad-spectrum 8-methoxy fluoroquinolone, gatifloxacin. | the in vitro antibacterial spectrum of gatifloxacin was compared with those of ciprofloxacin and ofloxacin. gatifloxacin was two- to four-fold more potent than comparator quinolones against staphylococci, streptococci, pneumococci and enterococci (gatifloxacin mic90s, < or =1 mg/l, except 4 mg/l against methicillin-resistant staphylococcus aureus and enterococcus faecium). gatifloxacin was two-fold less potent than ciprofloxacin, and the same as or two-fold more potent than ofloxacin against ent ... | 2000 | 10747819 |
| evaluation of antimicrobial treatment in mechanically ventilated patients with severe chronic obstructive pulmonary disease exacerbations. | to study microbial and susceptibility patterns and antimicrobial treatment responses in patients with severe, acute exacerbations of chronic obstructive pulmonary disease requiring mechanical ventilation. | 2000 | 10752816 |
| xanthomonas maltophilia infection in chronic peritoneal dialysis patients. | xanthomonas maltophilia infection has only been occasionally reported in patients receiving chronic peritoneal dialysis. we describe four cases of xanthomonas maltophilia infection associated with chronic peritoneal dialysis. two patients presented with peritonitis and two with exit site infection. all patients were diabetics, who immediately prior to the study had not received antibiotic therapy. failure to respond to multiple antibiotic therapy resulted in catheter removal in both patients wit ... | 2000 | 10757274 |
| comparison of aflp and rep-pcr genomic fingerprinting with dna-dna homology studies: xanthomonas as a model system. | the genus xanthomonas contains a large number of strains, which have been characterized by a variety of phenotypic and genotypic classification methods. the xanthomonas collection constitutes one of the largest groups of bacteria that have been characterized phylogenetically by dna-dna homology studies and genomic fingerprinting. presently, a total genomic dna-dna homology value of 70% represents an internationally accepted criterion to define bacterial species levels. however, the complexity of ... | 2000 | 10758874 |
| xanthomonas maltophilia endophthalmitis after cataract surgery. | 2000 | 10766146 | |
| automated surgical equipment requires routine disinfection of vacuum control manifold to prevent postoperative endophthalmitis. | contamination of automated surgical equipment is a potential source of postoperative endophthalmitis. the effect of disinfecting the unsterile vacuum control manifold (vcm) on contamination of the aspiration fluid was studied. | 2000 | 10768329 |
| stenotrophomonas maltophilia pneumonia in a premature infant. | stenotrophomonas (xanthomonas) maltophilia is an aerobic, non-fermentative, gram-negative bacillus that is generally considered an opportunistic pathogen. infections due to s. maltophilia have become increasingly important in the hospital environment. patients compromised by debilitating illnesses, surgical procedures or indwelling vascular catheters are most prone to s. maltophilia infections. to our knowledge, we report the first case of s. maltophilia pneumonia in a premature infant of 31 wee ... | 1999 | 10770672 |
| structures of the o21 and o25 antigens of stenotrophomonas maltophilia. | the o-specific side-chain polymers from stenotrophomonas maltophilia serogroups o21 and o25 were isolated from the lipopolysaccharides of the reference strains. the o21 polymer contained d-arabinose, 2-amino-2-deoxy-d-glucose and 2-amino-2-deoxy-d-galactose in equal proportions. methylation analysis and nmr spectroscopy showed that the polysaccharide is based on a branched trisaccharide repeating unit of the structure shown below. the o25 polymer is linear with a disaccharide repeating unit iden ... | 2000 | 10782291 |
| use of an enzyme-linked lectinsorbent assay to monitor the shift in polysaccharide composition in bacterial biofilms. | an enzyme-linked lectinsorbent assay (ella) was developed for quantification and characterization of extracellular polysaccharides produced by 1- and 4-day biofilms of 10 bacterial strains isolated from food industry premises. peroxidase-labeled concanavalin a (cona) and wheat germ agglutinin (wga) were used, as they specifically bind to saccharide residues most frequently encountered in biofilms matrices: d-glucose or d-mannose for cona and n-acetyl-d-glucosamine or n-acetylneuraminic acid for ... | 2000 | 10788349 |
| comparative evaluation of the in vitro activity of three combinations of beta-lactams with beta-lactamase inhibitors: piperacillin/tazobactam, ticarcillin/clavulanic acid and ampicillin/sulbactam. | recently, two new combinations of beta-lactam antibiotics with beta-lactamase inhibitors became commercially available in brazil: piperacillin/tazobactam and ampicillin/sulbactam. this study was designed to assess and compare the in-vitro activity of these new compounds, as well as that of ticarcillin/clavulanic acid, against bacteria isolated in our environment. a total of 749 bacteria isolated at são paulo hospital were tested using the disk diffusion method, in compliance with nccls standardi ... | 2000 | 10788842 |
| use of the midi-fame technique to characterize groundwater communities. | fatty acid methyl ester (fame) profiles were identified directly from groundwater microbial communities concentrated on and extracted with polycarbonate filters. the sensitivity of this direct extraction method was determined using pure cultures of acinetobacter junii, pseudomonas putida and stenotrophomonas maltophilia. a minimum concentration of 107 cells filter-1 was required to identify the predominant fatty acids from each culture. however, at least 3.7 x 109 cells filter-1 were required to ... | 2000 | 10792531 |
| microbial degradation and detoxification of high molecular weight polycyclic aromatic hydrocarbons by stenotrophomonas maltophilia strain vun 10,003. | the ability of stenotrophomonas maltophilia strain vun 10,003 to degrade and detoxify high molecular weight polycyclic aromatic hydrocarbons (pahs) was evaluated in a basal liquid medium. using high cell density inocula of strain vun 10,003, the concentration of pyrene, fluoranthene, benz[a]anthracene, benzo[a]pyrene, dibenz[a, h]anthracene and coronene decreased by 98, 45, 26, 22, 22 and 55% over periods ranging from 5 to 42 d. when a pah mixture containing three- to seven-ring compounds was us ... | 2000 | 10792670 |
| time-kill studies of tea tree oils on clinical isolates. | tea tree oil has recently emerged as an effective topical antimicrobial agent active against a wide range of organisms. tea tree oil may have a clinical application in both the hospital and community, especially for clearance of methicillin-resistant staphylococcus aureus (mrsa) carriage or as a hand disinfectant to prevent cross-infection with gram-positive and gramnegative epidemic organisms. our study, based on the time-kill approach, determined the kill rate of tea tree oil against several m ... | 2000 | 10797086 |
| field investigations of bacterial contaminants and their effects on extended porcine semen. | field investigations (n=23) were made over a 3-yr period at north american boar studs and farms in which the primary complaint was sperm agglutination in association with decreased sperm longevity of extended semen, and increased regular returns to estrus and/or vaginal discharges across parity. microscopic examination of extended semen from these units revealed depressed gross motility (usually <30%), sperm agglutination, and sperm cell death occurring within 2 d of semen collection and process ... | 2000 | 10798493 |
| a comparative analysis of patients with early-onset vs late-onset nosocomial pneumonia in the icu setting. | to compare the clinical outcomes of critically ill patients developing early-onset nosocomial pneumonia (np; ie, within 96 h of icu admission) and late-onset np (ie, occurring after 96 h of icu admission). | 2000 | 10807834 |
| septic arthritis caused by stenotrophomonas maltophilia in a patient with acquired immunodeficiency syndrome. | 2000 | 10812507 | |
| [prevalence of bacterial contamination of standard platelet units: prospective study]. | platelet concentrates contaminated with bacteria are a main source of transfusion-associated sepsis. several studies have reported a very wide incidence (0-10%) of contamination. the aim of this study is to assess the prevalence of bacterial contamination of standard platelet units at the regional blood transfusion center in casablanca. | 2000 | 10812660 |
| the legionella (fluoribacter) gormanii metallo-beta-lactamase: a new member of the highly divergent lineage of molecular-subclass b3 beta-lactamases. | a metallo-beta-lactamase determinant was cloned from a genomic library of legionella (fluoribacter) gormanii atcc 33297(t) constructed in the plasmid vector pacyc184 and transformed into escherichia coli dh5alpha, by screening for clones showing a reduced susceptibility to imipenem. the product of the cloned determinant, named fez-1, contains a 30-kda polypeptide and exhibits an isoelectric ph of 7.6. sequencing revealed that fez-1 is a molecular-class b beta-lactamase which shares the closest s ... | 2000 | 10817705 |
| in vitro activities of membrane-active peptides alone and in combination with clinically used antimicrobial agents against stenotrophomonas maltophilia. | the in vitro activities of buforin ii, cecropin p1, and magainin ii, alone and in combination with six clinically used antimicrobial agents, against 12 clinical isolates of stenotrophomonas maltophilia were investigated. antimicrobial activities were measured by mic and time-kill studies. the isolates were susceptible to the peptides at concentrations in the range of 0.50 to 16 microg/ml. synergy was observed when the peptides were combined with polymyxin e, meropenem, ceftazidime, piperacillin, ... | 2000 | 10817738 |
| activities of taurolidine in vitro and in experimental enterococcal endocarditis. | in vitro, the antimicrobial agent taurolidine inhibited virtually all of the bacteria tested, including vancomycin-resistant enterococci, oxacillin-resistant staphylococci, and stenotrophomonas maltophilia, at concentrations between 250 and 2,000 microg/ml. taurolidine was not effective in experimental endocarditis. while it appears unlikely that this antimicrobial would be useful for systemic therapy, its bactericidal activity and the resistance rates found (<10(-9)) are favorable indicators fo ... | 2000 | 10817739 |
| in vitro activity of gemifloxacin against a broad range of recent clinical isolates from the usa. | the antibacterial potencies of gemifloxacin (sb-265805) and 13 comparator compounds were determined by broth microdilution against a panel of 645 gram-positive and 995 gram-negative organisms collected from various usa sites. time-kill studies were performed and postantibiotic effect (pae) was determined for several organisms using trovafloxacin and ciprofloxacin as comparator compounds. based on mic(90)s, gemifloxacin was the most potent compound tested against gram-positive isolates: streptoco ... | 2000 | 10824027 |
| molecular investigation of stenotrophomonas maltophilia isolates exhibiting rapid emergence of ticarcillin-clavulanate resistance. | the prevalence of stenotrophomonas maltophilia resistance to ticarcillin-clavulanate has dramatically increased in our hospital over the past few years. sixty-six clinical isolates, collected from 61 patients, were tested for antibiotic susceptibility and genotyped by two different enterobacterial repetitive intergenic consensus-pcr (eric-pcr) systems. although two small outbreaks were identified in the medical intensive care unit, the remaining 58 strains generated 48 different pcr patterns. th ... | 2000 | 10833341 |
| bacteraemia caused by non-glucose-fermenting gram-negative bacilli and aeromonas species in patients with haematological malignancies and solid tumours. | the clinical characteristics and outcome of bacteraemia caused by non-glucose-fermenting gram-negative bacilli and aeromonas spp. were examined in 115 adults with haematological malignancies or solid tumours. the most aggressive pathogens were pseudomonas aeruginosa, stenotrophomonas maltophilia, aeromonas spp., acinetobacter spp. and burkholderia cepacia, all of which caused either septic syndrome or pneumonia in more than 40% of cases. pseudomonas aeruginosa was involved less often in catheter ... | 2000 | 10834826 |
| typing of 154 clinical isolates of stenotrophomonas maltophilia by pulsed-field gel electrophoresis and determination of the in vitro susceptibilities of these strains to 28 antibiotics. | 2000 | 10837454 | |
| novel online infusate-assisted dialysis system performs microbiologically safely. | administration of adequate amounts of commercial infusion fluids renders modern convective dialysis modalities, such as hemodiafiltration, labor-intensive and costly. preparation of infusate by cold sterilization of dialysis fluid, which is abundantly available, and its immediate (online) use, in contrast, enables a large volume fluid exchange in a cost-effective manner. recent developments aimed at more hygienic and user-friendly online systems with increased operational flexibility. as a resul ... | 2000 | 10848672 |
| stenotrophomonas maltophilia endophthalmitis after intraocular lens implantation. | stenotrophomonas maltophilia is an opportunistic, gram-negative bacillus. endophthalmitis induced by s. maltophilia has been described in only two cases after intraocular lens implantation. we report s. maltophilia endophthalmitis in two patients with diabetes mellitus after intraocular lens implantation and compare the characteristics of the s. maltophilia-induced endophthalmitis with two previous cases. | 2000 | 10853927 |
| stenotrophomonas maltophilia d457r contains a cluster of genes from gram-positive bacteria involved in antibiotic and heavy metal resistance. | a cluster of genes involved in antibiotic and heavy metal resistance has been characterized from a clinical isolate of the gram-negative bacterium stenotrophomonas maltophilia. these genes include a macrolide phosphotransferase (mphbm) and a cadmium efflux determinant (cada), together with the gene cadc coding for its transcriptional regulator. the cadc cada region is flanked by a truncated is257 sequence and a region coding for a bin3 invertase. despite their presence in a gram-negative bacteri ... | 2000 | 10858330 |
| molecular and biochemical heterogeneity of class b carbapenem-hydrolyzing beta-lactamases in chryseobacterium meningosepticum. | although the carbapenem-hydrolyzing beta-lactamase (chbetal) blab-1 is known to be in chryseobacterium meningosepticum nctc 10585, a second chbetal gene, bla(gob-1), was cloned from another c. meningosepticum clinical isolate (pint). the g+c content of bla(gob-1) (36%) indicated the likely chromosomal origin of this gene. its expression in escherichia coli dh10b yields a mature chbetal with a pi of 8.7 and a relative molecular mass of 28.2 kda. in e. coli, gob-1 conferred resistance to narrow-sp ... | 2000 | 10858348 |
| risk factors for emergence of stenotrophomonas maltophilia in cystic fibrosis. | the number of patients with cystic fibrosis (cf) whose sputum culture has yielded stenotrophomonas maltophilia has increased in the last 5 years at st. christopher's hospital for children. we conducted a case-control study to determine risk factors for recovery of s. maltophilia in respiratory secretions from patients with cf. we reviewed the outpatient and inpatient records of patients colonized with s. maltophilia between 1993 and 1997, and of age-matched (at time of initial recovery of s. mal ... | 2000 | 10862157 |
| cefditoren activity against nearly 1000 non-fastidious bacterial isolates and the development of in vitro susceptibility test methods. | cefditoren (formerly me-1206) is an investigational, orally administered cephalosporin ester with bactericidal activity against many gram-positive and -negative organisms. cefditoren potency against nearly 1000 non-fastidious species was determined by national committee for clinical laboratory standards (nccls) reference broth microdilution and standardized disk diffusion methods. against staphylococci, usable cefditoren activity was completely correlated with oxacillin with respect to potency a ... | 2000 | 10863109 |
| in vitro activity of eight fluoroquinolones against multiresistant stenotrophomonas maltophilia. | we studied the activity of eight fluoroquinolones (norfloxacin, ciprofloxacin, grepafloxacin, trovafloxacin, gatifloxacin, clinafloxacin, pd-117596 and pd-138312) against 58 multiresistant stenotrophomonas maltophilia clinical strains. norfloxacin was the least active drug (mic(90) = 128 mg/l). grepafloxacin, trovafloxacin and gatifloxacin activity was moderately higher (mic(90) = 8 mg/l) than ciprofloxacin (mic(90) = 16 mg/l). the best activity was shown by clinafloxacin and pd-138312 (mic(90) ... | 1999 | 10878514 |
| salt adaptation in pseudomonads: characterization of glucosylglycerol-synthesizing isolates from brackish coastal waters and the rhizosphere. | the compatible solute glucosylglycerol (gg) is widespread among cyanobacteria, but, until now, has been reported for only two species of heterotrophic bacteria. about 120 bacterial isolates from coastal regions of the baltic sea were screened by hplc for their ability to synthesize gg. positive isolates (26) were grouped by sds-page of whole-cell proteins and representative strains of each group were investigated by sequencing their 16s rrna genes and phenotypic characterization. all gg-synthesi ... | 2000 | 10879976 |
| occurrence and multiple antibiotic resistance profiles of non-fermentative gram-negative microflora in five brands of non-carbonated french bottled spring water. | five brands of french bottled mineral water were analyzed by heterotrophic plate counts (hpc) and for the presence of multiple antibiotic resistant bacteria. hpc at 22 degrees c were around 10(4) colony forming units ml(-1) on r2a medium. enumeration on pca/10, mh, and especially pca and king b media was less efficient. at 37 degrees c, hpc were two to three orders of magnitude less than at 22 degrees c. moreover, phenotypic diversity (7 to 15 phenotypes) was optimal on r2a incubated at 22 degre ... | 2000 | 10882437 |
| factors influencing protease production by two antarctic strains of stenotrophomonas maltophilia. | the influence of culture medium buffer capacity, the supplementation of culture medium with l-ala and the requirement of calcium for exoprotease production by antarctic psychrotrophic stenotrophomonas maltophilia strains ant-1-1 and ant-7-1 were examined. when increasing concentrations of calcium chloride (0 to 0.3 g l-1) were added to culture media, maximum protease production yields increased 70-75% (ant-1-1) and 50% (ant-7-1), while biomass levels showed little difference. calcium was also ne ... | 2000 | 10885004 |
| [accumulation and efflux of quinolones by clinical isolates of stenotrophomonas maltophilia]. | the presence of quinolone efflux pumps was analyzed in clinical isolates of stenotrophomonas maltophilia. the presence of the protein omp54, which is associated with the expression of multidrug resistant systems, was also tested. western blot analysis of outer membrane proteins demonstrated that omp54 was expressed in strains with high-level resistance to quinolones, whereas those strains with low mic values did not express this protein. this result shows that omp54 has an important role in the ... | 2000 | 10918091 |
| evidence of nosocomial stenotrophomonas maltophilia cross-infection in a neonatology unit analyzed by three molecular typing methods. | 2000 | 10926387 | |
| faucet aerators: a source of patient colonization with stenotrophomonas maltophilia. | 2000 | 10926715 | |
| overproduction of an inducible extracellular serine protease improves biological control of pythium ultimum by stenotrophomonas maltophilia strain w81. | stenotrophomonas maltophilia w81 can protect sugar beet against pythium:-mediated damping-off disease through the production of an extracellular protease. here, the proteolytic enzyme of w81 was purified by anion-exchange chromatography and characterized as a serine protease. the purified enzyme was fungicidal against pythium: ultimum in vitro. its synthesis was inducible by casein in w81, and mutagenesis of this strain using the luciferase (luxab) reporter transposon tn5-764cd resulted in the i ... | 2000 | 10931911 |
| identification of bacteria in water for pharmaceutical use. | different systems for the obtention of water used in biopharmaceutical industry were characterized from the bacteriological point of view. determination of aerobic mesophilic microorganisms was performed; as well as the isolation of contaminant microorganisms for what the techniques of membrane filtration was used. for the identification of the more representative species there were made conventional biochemical tests and quick systems: api. the results show that water serving as tap water for p ... | 1998 | 10932743 |
| [non-fermenting gram-negative bacilli: distribution in clinical specimens and antimicrobial susceptibility]. | in the present work was studied the prevalence, distribution in clinical specimens, and antimicrobial susceptibility of non-fermentative gram-negative bacilli (nfgnb) from patients attended at hospital "angela i. de llano" (corrientes, argentina). a total of 125 strains of nfgnb were recovered from various clinical specimens from july, 1997 to december, 1998. isolates were identified by classical biochemical tests. drug sensitivity was performed by standard methods with cefotaxime (ctx), ceftazi ... | 1999 | 10932769 |
| in vitro activity of newer fluoroquinolones against stenotrophomonas maltophilia. | 2000 | 10933670 | |
| phylogenetic affiliation of the pseudomonads based on 16s rrna sequence. | the broad and vague phenotypic definition allowed the genus pseudomonas to become a dumping ground for incompletely characterized polarly flagellated, gram-negative, rod-shaped, aerobic bacteria, and a large number of species have been accommodated in the genus pseudomonas. the 16s rrna sequences of 128 valid and invalid pseudomonas species, which included almost valid species of the genus pseudomonas listed in the approved lists of bacterial names, were obtained: sequences of 59 species were de ... | 2000 | 10939664 |
| use of 'locked-in' antibiotic to treat an unusual gram-negative hemodialysis catheter infection. | a 37-year-old woman on maintenance hemodialysis for 3 years had multiple vascular access failures due to antiphospholipid syndrome. she was dialyzed via a tunneled left subclavian catheter, but after 1 year developed chills and fever during each dialysis session. blood cultures grew out xanthomonas maltophilia sensitive to ceftazidime and ciprofloxacin. intravenous administration of both antibiotics failed to eradicate infection. we added 'locked-in' ceftazidime, instilling it daily into the cat ... | 2000 | 10940747 |
| transcatheter embolization of mycotic aneurysm of the subclavian artery with metallic coils. | mycotic aneurysms of the subclavian artery are rare. this report describes an experience of 2 rare cases in which transcatheter embolization with metallic coils was performed for the management of these lesions alternative to surgery. two patients who had been treated with chemotherapy for malignant neoplasms were diagnosed as having mycotic aneurysms of the left subclavian artery. the causes of these lesions were presumed to be the invasion of the arterial wall by the pulmonary abscess in case ... | 2000 | 10952342 |
| [bacteremia caused by stenotrophomonas maltophilia: epidemiology, clinical features, and prognosis factors]. | the epidemiologic, clinical and prognostic characteristics of stenotrophomonas maltophilia bacteremias for a six-year period (january 1993 to december 1998) were reviewed. twenty episodes of s. maltophilia bacteremia were include, which represented 0.62% of all bacteremias caused by gram-negative rods during that period. seventy percent of bacteremias were clinically significant. the most common predisposing factors were the previous use of antimicrobial agents and the presence of vascular cathe ... | 2000 | 10953584 |
| endocarditis caused by stenotrophomonas maltophilia. | stenotrophomonas maltophilia is frequently isolated from hospital environments. in recent years, it has been reported that this bacterium is causing hospital infections at increasing rates and is gaining importance because of its multiple resistance. although it has been related to several infections, endocarditis caused by s. maltophilia is rarely encountered. in this case study, endocarditis in a 40-y-old man with a history of aortic valve replacement is presented. blood cultures revealed s. m ... | 2000 | 10959657 |
| [prevalence of microbiota in the digestive tract of wild females of lutzomyia longipalpis lutz & neiva, 1912) (diptera: psychodidae)]. | we dissected the digestive tract of 245 females in pools of 35 flies forming 7 groups. these flies were lutzomyia longipalpis originating from lapinha cave, lagoa santa, minas gerais. out of the 8 species of bacteria isolated there was a predominancy of gram negative bacterias (gnb) in the group of non-fermenters of sugar belonging to the following species: acinetobacter lwoffii, stenotrophomonas maltophilia, pseudomonas putida and flavimonas orizihabitans. the group of gnb fermenters were: ente ... | 2000 | 10967602 |
| the use of silica gel prepared by sol-gel method and polyurethane foam as microbial carriers in the continuous degradation of phenol. | a mixed microbial culture was immobilized by entrapment into silica gel (sg) and entrapment/ adsorption on polyurethane foam (pu) and ceramic foam. the phenol degradation performance of the sg biocatalyst was studied in a packed-bed reactor (pbr), packed-bed reactor with ceramic foam (pbrc) and fluidized-bed reactor (fbr). in continuous experiments the maximum degradation rate of phenol (q(s)max) decreased in the order: pbrc (598 mg l(-1) h(-1)) > pbr (pu, 471 mg l(-1)h(-1)) > pbr(sg, 394 mg l(- ... | 2000 | 10968628 |
| stenotrophomonas maltophilia endocarditis of prosthetic aortic valve: report of a case and review of literature. | stenotrophomonas maltophilia (previously known as xanthomonas maltophilia and pseudomonas maltophilia ) is an aerobic, nonfermenting, gram-negative bacillus, which has emerged as a serious nosocomial pathogen in patients with compromised immunity. it is a rare cause of endocarditis with only 20 cases previously reported in medical literature. the risk factors associated with s maltophilia endocarditis include intravenous drug abuse, dental treatment, previous cardiac surgery, and infected intrav ... | 2000 | 10986530 |
| stenotrophomonas maltophilia infection in children. | 2000 | 11001113 | |
| investigation of an outbreak of gram-negative bacteremia among hematology-oncology outpatients. | to identify risk factors associated with an outbreak of gram-negative bacteremia (gnb). | 2000 | 11001264 |
| bacteremia due to stenotrophomonas maltophilia in patients with hematologic malignancies. | predisposing factors, clinical characteristics, and antimicrobial treatment of 37 hematology patients with stenotrophomonas maltophilia bacteremia who were seen at the department of hematology of the university la sapienza (rome) from 1987 to 1996 were evaluated. the results were compared with a control group of patients with pseudomonas aeruginosa bacteremia. profound neutropenia was more prolonged in the s. maltophilia group (p=.025), severe cellulitis occurred only in s. maltophilia-infected ... | 2000 | 11017819 |
| design, synthesis, and sar of novel carbapenem antibiotics with high stability to xanthomonas maltophilia oxyiminocephalosporinase type ii. | racemic cis-6-(phenylacetamido)carbapenem (21), 2-hydroxycarbonyl-cis-6-(phenylacetamido)carbapenem (22), 2-methoxycarbonyl-cis-6-(phenylacetamido)carbapenem (30), 2-methoxycarbomethyl-cis-6-(phenylacetamido)carbapenem (33), 2-hydroxyethyl-cis-6-(phenylacetamido)carbapenem (34), and 2-acetoxyethyl-cis-6-(phenylacetamido)carbapenem (35) were synthesized. formation of the carbapenem nuclei in 21, 22, and 30 involved dehydrophosphonation of the corresponding 2-diphenylphosphono-6-(phenylacetamido)c ... | 2000 | 11020277 |
| cloning and characterization of smedef, a novel multidrug efflux pump from stenotrophomonas maltophilia. | stenotrophomonas maltophilia is a nosocomial bacterial pathogen intrinsically resistant to several antibiotics. the mechanisms involved in this intrinsic multiresistance phenotype are poorly understood. a library of chromosomal dna from a spontaneous multidrug-resistant s. maltophilia d457r mutant (a. alonso and j. l. martinez, antimicrob. agents chemother. 41:1140-1142, 1997) was screened for complementation of erythromycin susceptibility on an antibiotic-hypersusceptible escherichia coli delta ... | 2000 | 11036026 |
| carbapenemases: a problem in waiting? | carbapenems are stable to most prevalent beta-lactamases, and chromosomal carbapenemases are restricted to stenotrophomonas maltophilia, to a few bacteroides fragilis, and to rare pathogens. nevertheless, an acquired metallo-beta-lactamase called imp-1 is beginning to emerge in pseudomonas aeruginosa and enterobacteriaceae isolates in japan, and has also been found in isolates from singapore. furthermore, imp-producing acinetobacter spp. have been identified in italy and hong kong. recently a se ... | 2000 | 11050448 |
| enrichment and isolation of non-specific aromatic degraders from unique uncontaminated (plant and faecal material) sources and contaminated soils. | microbial analysis of contaminated soil and uncontaminated plant and faecal material resulted in the enrichment of a number of microbial communities capable of utilizing a range of environmental pollutants. growth was observed on polycyclic aromatic hydrocarbons, polychlorinated biphenyls, heterocyclic aromatic compounds and organochlorine pesticides. however, none of the communities could grow on pentachlorophenol. pure cultures were isolated from microbial communities using phenanthrene and py ... | 2000 | 11054168 |
| influence of an extended incubation period on values of minimum inhibitory concentrations of antibiotics in stenotrophomonas maltophilia clinical strains. | in 106 stenotrophomonas maltophilia clinical strains the susceptibility to 19 kinds of antibiotics was tested by the broth dilution micromethod at 24 h and 48 h incubation. isolated strains demonstrated the lowest frequency of resistance to cotrimoxazole (7.5% of resistant strains at 24 h incubation and 18.9% at 48 h), ofloxacin (13.2% and 30.2%), ciprofloxacin (19.8% and 50.9%) and to cefoperazone/sulbactam (20.8% and 37.7%). the smallest growth of the number of resistant strains after extended ... | 2000 | 11061630 |
| antibacterial spectrum of a novel des-fluoro(6) quinolone, bms-284756. | the in vitro spectrum of a novel des-fluoro(6) quinolone, bms-284756, was compared with those of five fluoroquinolones (trovafloxacin, moxifloxacin, levofloxacin, ofloxacin, and ciprofloxacin). bms-284756 was among the most active and often was the most active quinolone against staphylococci (including methicillin-resistant strains), streptococci, pneumococci (including ciprofloxacin-nonsusceptible and penicillin-resistant strains), and enterococcus faecalis. bms-284756 inhibited approximately 6 ... | 2000 | 11083639 |
| partial degradation of p-aminoazobenzene by a defined mixed culture of bacillus subtilis and stenotrophomonas maltophilia. | a defined mixed culture of bacillus subtilis and stenotrophomonas maltophilia was used to accomplish the partial biodegradation of the azo-dye p-aminoazobenzene (paab). kinetic experiments were conducted, under aerobic conditions, to study the mineralization of p-aminoazobenzene by the above-defined mixed culture, under aerobic conditions. the combination of two previously developed models, (zissi et al., 1997), which describes paab biodegradation by bacillus subtilis into aniline and p-phenylen ... | 2001 | 11084593 |
| waterborne nosocomial infections. | waterborne pathogens cause infections in health-care facilities. despite guidelines addressing these pathogens, outbreaks and pseudo-outbreaks continue to occur. we reviewed recent reports of infections caused by pseudomonas aeruginosa, stenotrophomonas maltophilia, chryseobacterium species, nontuberculous mycobacteria, and legionella species. mycobacterium avium complex (mac) infection in hiv patients has been linked to hospital water distribution systems; molecular subtyping showed that mac is ... | 2000 | 11095897 |
| nonrespiratory stenotrophomonas maltophilia infection at a children's hospital. | to describe stenotrophomonas maltophilia infection in children, we reviewed the medical records of patients with isolates from nonrespiratory sites and identified 85 episodes, 51 (60%) of which represented true infection. forty-two episodes (82.4%) were hospital acquired. commonly associated with s. maltophilia infection were underlying illness (in 90.2% of cases), previous hospitalizations (in 78.7%), previous antibiotic exposure (in 78.4%), and the presence of a central venous catheter (in 76. ... | 2000 | 11095997 |
| unusual respiratory bacterial flora in cystic fibrosis: microbiologic and clinical features. | pulmonary infections continue to be a significant source of morbidity and mortality among patients with cystic fibrosis. although our understanding of the pathogenesis and clinical consequences of pulmonary infections with pseudomonas aeruginosa has increased greatly in recent years, very little is known about potentially emerging pathogens such as burkholderia cepacia complex, stenotrophomonas maltophilia, alcaligenes xylosoxidans, and methicillin-resistant staphylococcus aureus. in this review ... | 2000 | 11100967 |
| identification and detection of stenotrophomonas maltophilia by rrna-directed pcr. | stenotrophomonas maltophilia has recently emerged as an important nosocomial pathogen in immunocompromised patients, in transplant recipients, and in persons with cystic fibrosis (cf). while this organism is nonpathogenic in healthy individuals, it is increasingly associated with morbidity and mortality in susceptible populations. recent studies have indicated that for approximately 10% of cf patients with moderate lung disease, s. maltophilia can be cultured from respiratory tract secretions. i ... | 2000 | 11101555 |
| a tem-2beta-lactamase encoded on an active tn1-like transposon in the genome of a clinical isolate of stenotrophomonas maltophilia. | a constitutively expressed beta-lactamase gene from a clinical isolate of stenotrophomonas maltophilia, j675ia, has been cloned. its dna sequence is almost identical to that of bla(tem2) (one nucleotide change) and the expressed enzyme is a bush type 2a penicillinase with an amino acid sequence identical to that of tem-2. the bla(tem) gene was present within a novel tn1/tn3-type transposon in the genome of isolate j675ia and the transposon was able to mobilize bla(tem) on to the broad host-range ... | 2000 | 11102404 |
| [sterilization of phacoemulsification and vitrectomy instruments. contamination and evaluation]. | contamination of automated surgical equipment is widely disregarded as a potential source of perioperative infection. we investigated the possibility of contamination of the aspiration fluid by the vacuum control manifold (vcm). the normal, unsterile internal vcm was compared with a modified external vcm that was regularly disinfected. | 2000 | 11105548 |