Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| in vitro activities of beta-lactam-beta-lactamase inhibitor combinations against stenotrophomonas maltophilia: correlation between methods for testing inhibitory activity, time-kill curves, and bactericidal activity. | the activities of ampicillin, ampicillin-sulbactam, amoxicillin, amoxicillin-clavulanic acid, ticarcillin, ticarcillin-clavulanic acid, piperacillin, piperacillin-tazobactam, aztreonam, and aztreonam-clavulanic against stenotrophomonas maltophilia strains for which the mics of penicillins and commercially available beta-lactam-beta-lactamase inhibitor combinations were higher than the breakpoints usually recommended for pseudomonas aeruginosa in commercially available broth microdilution methods ... | 1997 | 9420028 |
| in vitro and in vivo antibacterial activities of cs-834, a novel oral carbapenem. | cs-834 is a novel oral carbapenem antibiotic. this compound is an ester-type prodrug of the active metabolite r-95867. the antibacterial activity of r-95867 was tested against 1,323 clinical isolates of 35 species and was compared with those of oral cephems, i.e., cefteram, cefpodoxime, cefdinir, and cefditoren, and that of a parenteral carbapenem, imipenem. r-95867 exhibited a broad spectrum of activity covering both gram-positive and -negative aerobes and anaerobes. its activity was superior t ... | 1997 | 9420035 |
| susceptibility of 100 strains of stenotrophomonas maltophilia to three beta-lactams and five beta-lactam-beta-lactamase inhibitor combinations. | one hundred clinical strains of stenotrophomonas maltophilia were investigated for their susceptibility to ticarcillin, piperacillin, ceftazidime and five combinations with beta-lactamase inhibitors (clavulanic acid, tazocillin or sulbactam) at three concentrations; 26% of the strains were susceptible to ticarcillin, 23% to ceftazidime and 12% to piperacillin. eighty-two percent of the strains were susceptible to ticarcillin/clavulanic acid. in most cases, no synergy was observed with tazobactam ... | 1997 | 9421322 |
| microbiological and clinical aspects of infection associated with stenotrophomonas maltophilia. | the gram-negative bacterium stenotrophomonas maltophilia is increasingly recognized as an important cause of nosocomial infection. infection occurs principally, but not exclusively, in debilitated and immunosuppressed individuals. management of s. maltophilia-associated infection is problematic because many strains of the bacterium manifest resistance to multiple antibiotics. these difficulties are compounded by methodological problems in in vitro susceptibility testing for which there are, as y ... | 1998 | 9457429 |
| the significance of aerobic gram-negative bacilli in clinical specimens following orthotopic liver transplantation. | in a prospective study of 284 liver transplant patients, we sought associations between aerobic gram-negative bacillus acquisition or infection and 35 preoperative, perioperative, and postoperative variables. although the 128 (45%) who acquired aerobic gram-negative bacilli had longer admissions (p = 0.0001), no associations were found with pretransplant variables. fifty-three (41%) of the 128 acquired coliforms (e.g., escherichia coli, klebsiella spp., or enterobacter spp.), 50 (39%) acquired n ... | 1998 | 9457967 |
| susceptibilities of 97 strains of xanthomonas maltophilia to antibiotics and the effect of beta-lactamase inhibitors. | an agar dilution technique and the 'e' test were used to compare the antimicrobial activities of ticarcillin, ticarcillin-clavulanate, ampicillin, ampicillin-sulbactam, piperacillin, piperacillin-tazobactam, meropenem, biapenem, imipenem, levofloxacin, ofloxacin, ciprofloxacin, ceftazidime and cefepime against 97 clinical isolates of xanthomonas maltophilia. intermediate susceptibility breakpoints for members of the family enterobacteriaceae were used. results were analysed as minimum inhibitory ... | 1997 | 9467924 |
| ventilator-associated pneumonia caused by potentially drug-resistant bacteria. | to determine risk factors for ventilator-associated pneumonia (vap) caused by potentially drug-resistant bacteria such as methicillin-resistant staphylococcus aureus, pseudomonas aeruginosa, acinetobacter baumannii, and/or stenotrophomonas maltophilia, 135 consecutive episodes of vap observed in a single icu over a 25-mo period were prospectively studied. for all patients, vap was diagnosed based on results of bronchoscopic protected specimen brush (> or = 10(3) cfu/ml) and bronchoalveolar lavag ... | 1998 | 9476869 |
| synergistic activity of trovafloxacin with other agents against gram-positive and -negative organisms. | the synergistic activity of trovafloxacin with other agents against 55 gram-positive and -negative bacteria was determined by checkerboard titration. synergistic fractional inhibitory concentration (fic) indices (< or = 0.5) were seen in two methicillin-susceptible and one methicillin-resistant staphyloccocus aureus with teicoplanin, one of each of the latter two with vancomycin; one methicillin-resistant coagulase-negative staphylococcus with rifampin and one with fusidic acid; five stenotropho ... | 1998 | 9488833 |
| treatment with beta-lactams and superinfection with stenotrophomonas maltophilia: a historical cohort study could show us more. | 1998 | 9502503 | |
| in-vitro bactericidal efficacy of sub-mic concentrations of liposome-encapsulated antibiotic against gram-negative and gram-positive bacteria. | it has been shown previously that tobramycin encapsulated in fluid liposomes (composed of dipalmitoylphosphatidylcholine (dppc) and dimyristoylphosphatidylglycerol (dmpg)) eradicated mucoid pseudomonas aeruginosa in an animal model of chronic pulmonary infection. exponential cultures of p. aeruginosa, stenotrophomonas maltophila, burkholderia cepacia, escherichia coli and staphylococcus aureus were treated with (i) free tobramycin, (ii) sub-mic tobramycin encapsulated in dppc/dmpg liposomes, (ii ... | 1998 | 9511035 |
| [clinical significance of the determination of esbl (extended- spectrum beta-lactamase) production by gram-negative nosocomial bacteria]. | data on the incidence of multiresistant nosocomial bacterial strains of xanthomonas (stenotrophomonas) maltophilia, producing in addition to metallo-beta-lactamases also new types of broad spectrum beta-lactamases (esbl) called for analyses of the production of different types of enzymes. | 1998 | 9511267 |
| in vitro and in vivo antibacterial activities of cs-834, a new oral carbapenem. | cs-834 is a prodrug of the carbapenem r-95867, developed by sankyo co., ltd., tokyo, japan. to investigate the possibility that cs-834 may be the first carbapenem usable in an oral dosage form, its in vitro antibacterial activity (as r-95867) and in vivo antibacterial activity were compared with those of cefpodoxime proxetil, cefditoren pivoxil, cefdinir, ofloxacin, imipenem, and amoxicillin. r-95867 had high levels of activity against methicillin-susceptible staphylococci and streptococci, incl ... | 1998 | 9517932 |
| two nosocomial strains of stenotrophomonas maltophilia transferring antibiotic resistance to proteus mirabilis p-38 recipient strain. | in this report we describe a specific transfer of carbenicillin and cephaloridine resistance determinants from two different strains of stenotrophomonas maltophilia: no. 215 and 221 isolated from two critically ill patients treated in different intensive care units of a large university hospital in ostrava, czech republic. these strains were resistant to flouroquinolones and the following beta-lactam drugs: carbenicillin, cephaloridine, cefotaxime, ceftazidime, cefepime, imipenem, meropenem and ... | 1998 | 9531071 |
| activity of bacterial phosphomonoesterases in batch culture. | the phosphomonoesterases catalyse the hydrolysis of primary esters of phosphoric acid which help the bacteria to survive in phosphate stressed environment. ninety-five bacterial isolates were obtained from domestic sewage and industrial effluents of gelatine and soap factories at jabalpur on a medium enriched with phosphate and were screened for phosphatase production. the phosphatase producers were tentatively identified as escherichia coli, vibrio vulnificus, aeromonas hydrophila, staphylococc ... | 1998 | 9536656 |
| overexpression, purification, and characterization of the cloned metallo-beta-lactamase l1 from stenotrophomonas maltophilia. | the metallo-beta-lactamase l1 from stenotrophomonas maltophilia was cloned, overexpressed, and characterized by spectrometric and biochemical techniques. results of metal analyses were consistent with the cloned enzyme having 2 mol of tightly bound zn(ii) per monomer. gel filtration chromatography demonstrated that the cloned enzyme exists as a tightly held tetramer with a molecular mass of ca. 115 kda, and matrix-assisted laser desorption ionization and time-of-flight mass spectrometry indicate ... | 1998 | 9559809 |
| in vitro activity of cefpirome against microorganisms isolated in haematology, oncology and intensive care units in switzerland. | the in vitro activity of cefpirome, a new parenteral fourth-generation cephalosporin, was investigated in the 5 university hospitals of switzerland, and compared to 9 other antibiotics mainly used in hospitals, such as ceftazidime, ceftriaxone, cefotaxime, piperacillin, imipenem, gentamicin, vancomycin, ciprofloxacin and ofloxacin. a total number of 992 strains collected only from intensive care units and haematology-oncology units were tested by microdilution according to nccls. cefpirome showe ... | 1997 | 9571744 |
| identification of acinetobacters on blood agar in presence of d-glucose by unique browning effect. | a positive phenotypic characteristic of glucose-oxidizing acinetobacters was demonstrated with blood agar containing d-glucose. glucose-oxidizing acinetobacter baumannii, acinetobacter genospecies 3, acinetobacter lwoffii, and acinetobacter genospecies 13 sensu tjernberg and ursing caused a unique brown discoloration of media supplemented with 5% blood (of horse, sheep, or human origin) and an aldose sugar (0.22 m d-glucose, d-galactose, d-mannose, d-xylose, or lactose). the browning effect was ... | 1998 | 9574714 |
| characterization and sequence of the chryseobacterium (flavobacterium) meningosepticum carbapenemase: a new molecular class b beta-lactamase showing a broad substrate profile. | the metallo-beta-lactamase produced by chryseobacterium (formerly flavobacterium) meningosepticum, which is the flavobacterial species of greatest clinical relevance, was purified and characterized. the enzyme, named blab, contains a polypeptide with an apparent mr of 26000, and has a pi of 8.5. it hydrolyses penicillins, cephalosporins (including cefoxitin), carbapenems and 6-beta-iodopenicillanate, a mechanism-based inactivator of active-site serine beta-lactamases. the enzyme was inhibited by ... | 1998 | 9576862 |
| bottled water as a source of multi-resistant stenotrophomonas and pseudomonas species for neutropenic patients. | stenotrophomonas maltophilia is an emerging nosocomial pathogen in patients rendered neutropenic following cytoreductive therapy for malignancy. the identification of sources and routes of transmission of this bacterium is of importance in the development of strategies to prevent nosocomial acquisition of s. maltophilia. a previous study had shown high rates of faecal carriage of s. maltophilia amongst patients with haematological malignancy and, as bottled water was consumed by many patients, w ... | 1998 | 9582746 |
| interactions of beta-lactamases with sanfetrinem (gv 104326) compared to those with imipenem and with oral beta-lactams. | sanfetrinem is a trinem beta-lactam which can be administered orally as a hexatil ester. we examined whether its beta-lactamase interactions resembled those of the available carbapenems, i.e., stable to ampc and extended-spectrum beta-lactamases but labile to class b and functional group 2f enzymes. the comparator drugs were imipenem, oral cephalosporins, and amoxicillin. mics were determined for beta-lactamase expression variants, and hydrolysis was examined directly with representative enzymes ... | 1998 | 9593145 |
| molecular heterogeneity of the l-1 metallo-beta-lactamase family from stenotrophomonas maltophilia. | we have determined the nucleotide sequence of the blas gene encoding the carbapenem-hydrolyzing l-1 beta-lactamase from stenotrophomonas maltophilia gn12873. analysis of the dna and deduced amino acid sequences identified a product of 290 amino acids. comparisons of the l-1 amino acid sequence with those of other zinc beta-lactamases showed 88.6% identity with the l-1 enzyme from s. maltophilia iid1275 and less than 20% identity with other class b metalloenzymes. | 1998 | 9593158 |
| a standardized protocol for the rapid preparation of bacterial dna for pulsed-field gel electrophoresis. | a rapid method for the preparation of bacterial dna for pulsed-field gel electrophoresis was developed for gram-positive and gram-negative bacteria. this method was accomplished by reducing the time for the cell lysis reaction, restriction endonuclease digestion, and electrophoresis to 1, 1.5, and 18 h, respectively. the whole procedure from the initial bacterial culture plate to the final analysis of restriction fragments can be completed within 24 h. this rapid method was successfully achieved ... | 1998 | 9597387 |
| comparison of in vitro activity of trovafloxacin against gram-positive and gram-negative organisms with quinolones and beta-lactam antimicrobial agents. | the in vitro activity of trovafloxacin against 721 gram-negative and 498 gram-positive organisms was determined by the standard microdilution broth method using commercially prepared frozen microtiter plates. the activity of trovafloxacin was compared to ofloxacin, ciprofloxacin, amoxicillin/clavulanate, ampicillin/sulbactam (1:1), piperacillin/tazobactam, ceftriaxone, and imipenem. trovafloxacin had equal or greater activity compared with the other agents tested against citrobacter diversus, en ... | 1998 | 9597391 |
| xanthomonas maltophilia: an emerging pathogen in patients with hiv disease. | fifty-four episodes of xanthomonas maltophilia infection were observed in 52 hiv-infected patients out of 2062 assessed (2.52%) over a 6-year period: sepsis/bacteraemia in 44 cases, lower airways infection in 5 cases, urinary tract infection and pharyngitis in 2 cases each, and lymph node involvement in one patient. x. maltophilia represented the fourth most common non-mycobacterial bacterial pathogen responsible for bacteraemia in hiv-infected patients: 44 cases out of 721 diagnosed (6.1%). whe ... | 1998 | 9598746 |
| aztreonam-clavulanic acid synergy does not mean extended-spectrum beta-lactamase in stenotrophomonas maltophilia. | 1998 | 9598782 | |
| imipenem resistance in aerobic gram-negative bacteria. | a prospective study was undertaken to observe the emergence of resistance to imipenem, if any, among aerobic gram-negative bacteria. a total of 736 isolates were tested during 1994-95 and less than 1% of them were resistant to imipenem, whereas the next year ('95-'96) the rate increased to 11 of the 903 isolates tested. the resistant isolates during '94-'95 were all stenotrophomonas maltophilia whereas the spectrum of resistant bacterial species increased in '95-'96 to include pseudomonas aerugi ... | 1998 | 9603633 |
| antibiotic susceptibility of stenotrophomonas (xanthomonas) maltophilia: comparative (nccls criteria) evaluation of antimicrobial drugs with the agar dilution and the agar disk diffusion (bauer-kirby) tests. | ninety-six clinical isolates of stenotrophomonas maltophilia were examined with the agar dilution method for susceptibility to 19 antimicrobial drugs. doxycycline, cotrimoxazole, timentin, ofloxacin, fosfomycin, and piperacillin + tazobactam, in that order, inhibited the majority of strains. all isolates were resistant to nitrofurantoin. concurrent disk susceptibility (bauer-kirby method) testing, using currently valid nccls interpretative criteria for pseudomonas aeruginosa, uncovered a signifi ... | 1998 | 9612606 |
| a new approach to the treatment of xanthomonas maltophilia respiratory infection in a patient with cystic fibrosis. | 1998 | 9616541 | |
| men 10700, a new penem antibiotic: in-vitro activity and its correlation with beta-lactamase stability, pbp affinity and diffusion through the bacterial cell wall. | the in-vitro activity of men 10700, a novel penem, was compared with that of imipenem, ritipenem, ampicillin/sulbactam, cefotaxime, ciprofloxacin and amikacin against 1088 strains taken from 21 genera, including gram-negative, gram-positive and anaerobic bacteria. mic data showed that men 10700 was very active against staphylococci and streptococci (mic90 < or = 0.5 mg/l) and against most members of the enterobacteriaceae (mic90 < or = 2 mg/l), with reduced activity only against providencia stua ... | 1998 | 9630405 |
| bacterial-fungal biofilms in flowing water photo-processing tanks. | a complex seven species model community, including bacteria and fungi, was selected from organisms isolated from the walls of an industrial flowing water system. growth rates of the species were determined in single and mixed batch culture growth. the rates were found to be significantly higher in mixed culture for pseudomonas alcaligenes and flavobacterium indologenes and higher in single culture for xanthomonas maltophilia, rhodotorula glutinis and fusarium solani, whereas no significant diffe ... | 1998 | 9633659 |
| stenotrophomonas maltophilia in cystic fibrosis: incidence and prevalence. | stenotrophomonas maltophilia (sm) was recovered from 211 of 773 cystic fibrosis (cf) patients followed for at least one year, and seen between 1982 and 1994. yearly prevalence (5.6% to 8.7%) and incidence rates (1.6% to 5.7%) showed no trends. sm persistence varied greatly and was unlike that of pseudomonas aeruginosa. fifty percent of sm-positive patients had only one positive culture and only 24 (11%) remained chronically infected. although sm-positive patients were more likely to be hospitali ... | 1998 | 9635931 |
| extended spectrum beta-lactamase reactions in stenotrophomonas maltophilia. | 1998 | 9646115 | |
| molecular epidemiology of stenotrophomonas maltophilia isolated from clinical specimens from patients with cystic fibrosis and associated environmental samples. | stenotrophomonas maltophilia was isolated from the respiratory tracts of 41 (25%) of 163 children attending our pediatric cystic fibrosis unit between september 1993 and december 1995. the extents of s. maltophilia contamination of environmental sites frequented by these patients were investigated with a selective medium incorporating vancomycin, imipenem, and amphotericin b. eighty-two isolates of s. maltophilia were cultured from 67 different environmental sites sampled between january and jul ... | 1998 | 9650943 |
| fulminant hepatitis complicated by small intestine infection and massive hemorrhage. | a 34-year-old man diagnosed with fulminant hepatitis, caused by hepatitis b virus, and acute renal failure was referred to our hospital. after admission to the intensive care unit, the liver and renal failure were ameliorated. melena requiring transfusion occurred during the course of his illness. endoscopic examination demonstrated pseudomembranes, erosions, ulcers, and hemorrhage in the duodenum, the upper jejunum, and the terminal ileum, suggesting widespread lesions throughout the small inte ... | 1998 | 9658323 |
| reconstituted recombinant factor viii can be safely infused continuously for at least three days: it is a poor microbial growth medium. | reconstituted recombinant factor viii (fviiirec) loses little biologic activity at room temperature for up to seven days and continuous infusion is convenient, effective hemostatically and requires less fviiirec concentrate than treatment by conventional bolus injections. however, the potential for bacterial contamination, with proliferation to high levels that can cause bacteremia, is a concern with continuous infusion. we studied the growth properties at 4, 25 and 35 degrees c in reconstituted ... | 1998 | 9663704 |
| further studies of transferable antibiotic resistance in strains of pseudomonas aeruginosa from four clinical settings in three countries. | this paper describes transferability of antibiotic resistance determinants in clinical isolates of pseudomonas aeruginosa resistant to imipenem, cefotaxime and ceftazidime obtained from different clinical settings in three different countries. two strains of enterobacteriaceae (escherichia coli k-12 and proteus mirabilis p-38) and two strains of p. aeruginosa (pao and ml) were used as recipient strains. the conjugative transfer of resistance was very specific, i.e. donor strains of p. aeruginosa ... | 1998 | 9669646 |
| bacteremia and fungemia in pediatric versus adult cancer patients after chemotherapy: comparison of etiology, risk factors and outcome. | one hundred and eighteen (118) episodes of bacteremia and fungemia in children with cancer were compared to 401 episodes of bacteremia and fungemia in adults with cancer to assess differences in etiology, risk factors and outcome. a retrospective univariate analysis was performed of all episodes of bacteremia in national pediatric and adult cancer institutions appearing in 1990-1996. a total of 519 episodes of bacteremia were assessed and compared. both cancer centers differed in prophylactic an ... | 1998 | 9669650 |
| microbiology of sputum from patients at cystic fibrosis centers in the united states. | during a phase iii national collaborative study of aerosolized tobramycin (1 july 1995 through 30 september 1996), the microbiology of specimens from 595 patients at 69 cystic fibrosis (cf) centers was examined. samples from three screening visits were processed in a single laboratory by means of standardized techniques for identification and susceptibility testing. from 1,753 pretreatment specimens, 5,128 pathogens were isolated (average, 2.9/specimen). of the 3,936 pseudomonas aeruginosa isola ... | 1998 | 9675470 |
| activities of three quinolones, alone and in combination with extended-spectrum cephalosporins or gentamicin, against stenotrophomonas maltophilia. | the present study examined the activities of trovafloxacin, levofloxacin, and ciprofloxacin, alone and in combination with cefoperazone, ceftazidime, cefpirome, and gentamicin, against 100 strains of stenotrophomonas maltophilia by the mic determination method and by synergy testing of the combinations by the time-kill and checkerboard titration methods for 20 strains. the respective mics at which 50% and 90% of isolates were inhibited for the drugs used alone were as follows: trovafloxacin, 0.5 ... | 1998 | 9687397 |
| nosocomial outbreak of colonization and infection with stenotrophomonas maltophilia in preterm infants associated with contaminated tap water. | between march and may 1996 stenotrophomonas maltophilia was cultured from endotracheal aspirate samples from five preterm infants in a neonatal intensive care unit (nicu). four infants were superficially colonized, but a fifth died due to s. maltophilia septicaemia. s. maltophilia was cultured from tap water from three outlets in the nicu including one with a previously unnoticed defective sink drain. water from these outlets was used to wash the preterm infants. environmental and clinical s. ma ... | 1998 | 9692603 |
| activity of cefepime compared with other antibiotics against gram-positive bacteria and cefuroxime-resistant gram-negative bacteria. | cefepime is a new, parenteral, fourth-generation antibiotic that is stable in the presence of bush group 1 beta-lactamases. in vitro activity of cefepime, cefuroxime, ceftazidime, ciprofloxacin and imipenem against gram-positive cocci and cefuroxime-resistant gram-negative bacilli was studied. | 1998 | 9699393 |
| detection of bacteraemia in patients with fever and neutropenia using 16s rrna gene amplification by polymerase chain reaction. | episodes of fever and neutropenia are common complications of treatment for cancer. the use of prophylactic and early empirical antibiotics has reduced mortality but decreases the sensitivity of diagnostic tests based on culture. the aim of this study was to determine the potential of a broad diagnostic approach (eubacterial) based on 16s rrna gene amplification and sequencing to augment cultural methods of diagnosis of bacteraemia in patients with fever and neutropenia in a regional paediatric ... | 1998 | 9707307 |
| resistance mechanisms in pseudomonas aeruginosa and other nonfermentative gram-negative bacteria. | nonfermentative gram-negative bacilli are still a major concern in compromised individuals. by far the most important of these organisms is pseudomonas aeruginosa, although acinetobacter baumannii (previously acinetobacter calcoaceticus), stenotrophomonas maltophilia (previously pseudomonas and xanthomonas maltophilia), and burkholderia cepacia (previously pseudomonas cepacia) are also of substantative concern because of their similar high intrinsic resistances to antibiotics. the basis for the ... | 1998 | 9710677 |
| clinical problems posed by multiresistant nonfermenting gram-negative pathogens. | in this review i will briefly survey the role of pseudomonas aeruginosa, acinetobacter baumanii, stenotrophomonas maltophilia, and burkholderia cepacia as opportunistic pathogens. a common feature of these organisms is intrinsic resistance to multiple antibiotics. all of these organisms can be recovered from the environment, commonly cause device-related infections, are often resistant to disinfectants, and have the potential to spread from patient to patient via fomites or the hands of medical ... | 1998 | 9710680 |
| a relatively small change in sodium chloride concentration has a strong effect on adhesion of ocular bacteria to contact lenses. | adhesion of bacteria to hydrogel lenses is thought to be an initial step of ocular colonization allowing evasion of normal host defences. the salt concentration of media is an important parameter controlling microbial adhesion. salinity varies from 0.97% nacl equivalents in the open eye to 0.89% in the closed eye state. in this study, the effect of sodium chloride in the concentration range of 0.8-1.0% (w/v) nacl on adhesion of ocular bacteria to soft contact lenses was investigated using a stat ... | 1998 | 9717279 |
| bacteremia due to multiresistant gram-negative bacilli in neutropenic cancer patients: a case controlled study. | the aim of this study was to see if multiresistant gram-negative bacteremias (mrgnb) are associated with specific risk factors and/or higher mortality in comparison to sensitive gnb (sgnb). both groups, 51 patients and 102 controls, were matched for sex, age, underlying disease and neutropenia. in addition there were no significant differences in the incidence of cytotoxic chemotherapy administered, vascular catheter insertion and catheter as source of bacteremia and etiology of bacteremia. the ... | 1998 | 9720472 |
| infective endocarditis due to stenotrophomonas (xanthomonas) maltophilia. | stenotrophomonas maltophilia (formerly xanthomonas maltophilia) is a gram-negative bacillus increasingly associated with serious nosocomial infections. here, the case of a 69-year-old female patient who developed prosthetic valve endocarditis associated with this organism is described. a review of the literature revealed only 18 previous reports; eight involved native valves, the remainder prosthetic valves. most cases were associated with risk factors, including intravenous drug abuse (6 patien ... | 1998 | 9721966 |
| molecular identification of a stenotrophomonas species used in the bioassay for erythromycin in aquaculture samples. | a bacterial strain isolated from aquaculture pond slurry, which was extremely sensitive to erythromycin, was used to detect erythromycin at levels as low as 0.05 micrograms ml-1 in aquaculture water, sediments and soil samples. identification of the indicator organism was attempted by 16s rrna sequencing, biochemical profile, fatty-acid analysis and polymerase chain reaction (pcr). genbank comparison showed that the 16s rrna sequence of the strain was similar to those of more than 20 copies of x ... | 1998 | 9727203 |
| stenotrophomonas (xanthomonas) maltophilia: in vitro susceptibility to selected antimicrobial drugs, single and combined, with and without defibrinated human blood. | sixteen selected isolates of stenotrophomonas maltophilia varied in susceptibility to the combined phagocytic/serum bactericidal activity of fresh defibrinated human blood (65 vol%). four representative isolates (x1, x11, x25, and x50), which differed in susceptibility to cefepime, ceftazidime, rifampin, and timentin, were subjected to checkerboard microtiter broth dilution tests involving combinations of cefepime plus timentin, ceftazidime plus ofloxacin, cotrimoxazole plus timentin, rifampin p ... | 1998 | 9732143 |
| total nutrient admixtures appear safer than lipid emulsion alone as regards microbial contamination: growth properties of microbial pathogens at room temperature. | the extraordinary growth properties of most microorganisms in 10% and 20% lipid emulsions has led to the centers for disease control and prevention recommendation that if lipids are given through an i.v. line, the administration set should be replaced every 24 hours rather than the usual 72-hour interval used for crystalloid solutions, including those used for conventional total parenteral nutrition. for nearly 15 years, parenteral alimentation has been given as a total nutrient admixture (tna), ... | 1998 | 9739032 |
| the o7 antigen of stenotrophomonas maltophilia is a linear d-rhamnan with a trisaccharide repeating unit that is also present in polymers for some pseudomonas and burkholderia species. | the o antigen polymer recovered from the reference strain for stenotrophomonas (xanthomonas or pseudomonas) maltophilia serogroup o7, after mild acid hydrolysis of the lipopolysaccharide, was constructed from d-rhamnose. by means of chemical degradations and nmr studies, the repeating unit of the polymer was shown to be a linear trisaccharide with the structure -->2)-alpha-d-rhap-(1-->3)-alpha-d-rhap-(1-->3)-alpha-d-r hap-(1-->. the same repeating unit is present in the common antigen of pseudom ... | 1998 | 9741084 |
| [evaluation of antibacterial activities of various antibiotics against glucose non-fermentative gram-negative rods other than pseudomonas aeruginosa]. | mics of piperacillin, sulbactam/cefoperazone, minocycline (mino), gentamicin, amikacin, flomoxef, ceftazidime, cefozopran, cefsulodin and imipenem were determined, against 189 clinical isolated strains of glucose non-fermentative gram-negative rods (nfgnr; acinetobacter baumannii (44), alcaligenes faecalis (5), alcaligenes xylosoxidans (25), burkholderia cepacia (12), chryseobacterium indologenes (23), chryseobacterium meningosepticum (9), pseudomonas fluorescens (8), pseudomonas putida (12), st ... | 1998 | 9755431 |
| [bacteremia caused by stenotrophomonas maltophilia: a clinical-epidemiological study and resistance profile]. | over a 7-year period (1990-1996), the causal disease, predisposing factors, focus infection, clinical manifestations, complications and evolution of patients presenting bacteremia from stenotrophomonas maltophilia were analyzed retrospectively in a university hospital. a microbiological study was carried out to determine the percentage of positive blood cultures per episode and the characteristics of bacteremia and to evaluate the antibiotic susceptibility of the isolated strains. twenty-seven e ... | 1998 | 9795306 |
| the crystal structure of the l1 metallo-beta-lactamase from stenotrophomonas maltophilia at 1.7 a resolution. | the structure of the l1 metallo-beta-lactamase from the opportunistic pathogen stenotrophomonas maltophilia has been determined at 1.7 a resolution by the multiwavelength anomalous dispersion (mad) approach exploiting both the intrinsic binuclear zinc centre and incorporated selenomethionine residues. l1 is unique amongst all known beta-lactamases in that it exists as a tetramer. the protein exhibits the alphabeta/betaalpha fold found only in the metallo-beta-lactamases and displays several uniq ... | 1998 | 9811546 |
| antimicrobial interactions of trovafloxacin and extended-spectrum cephalosporins or azithromycin tested against clinical isolates of pseudomonas aeruginosa and stenotrophomonas maltophilia. | 1998 | 9818765 | |
| antimicrobial activity of merocyanine 540: a photosensitizing dye. | the antimicrobial activity of merocyanine 540 (mc 540), a photosensitizing dye previously used to purge malignant cells from autologous bone marrow grafts, was evaluated against a panel of gram-positive and gram-negative bacteria and candida albicans in the presence and absence of light. in the absence of light, mc 540 demonstrated no antibacterial activity against any of the organisms tested. when combined with increasing intervals of photoillumination, growth inhibition was observed with all g ... | 1998 | 9823532 |
| effect of oxidative stress on the biosynthesis of 2-c-methyl-d-erythritol-2,4-cyclopyrophosphate and isoprenoids by several bacterial strains. | in this study, the gram-negative bacteria xanthomonas campestris, xanthomonas maltophilia, and pseudomonas putida, facultative parasites of plants and animals, were shown to accumulate 2-c-methyl-d-erythritol-2,4-cyclopyrophosphate (mec) in response to benzyl-viologen-induced oxidative stress. corynebacterium ammoniagenes mutants capable of accumulating mec in the absence of an exogenous oxidative stress inducer were obtained. isoprenoid synthesis and mec synthesis in these and other bacteria we ... | 1998 | 9871022 |
| comparison of various in vitro susceptibility methods for testing stenotrophomonas maltophilia. | a total of 57 clinical isolates were screened by disk diffusion for a related pharmacodynamic study. testing was performed using national committee for clinical laboratory standards guidelines, except that results were interpreted at 16 to 18 h and 48 h. of the 57 isolates, 19 were randomly chosen for additional comparative susceptibility testing of five methods (disk diffusion, etest, alamar colorimetric broth microdilution, vitek, and microscan) and an in-house broth microdilution method. the ... | 1998 | 9884841 |
| [community-acquired bacteremia and pneumonia caused by stenotrophomonas maltophilia in a patient with aids]. | 1998 | 9887635 | |
| [bacteremia with granulocytopenia--microbiology and empiric antibiotic treatment]. | this article sums up a retrospective analysis of 84 episodes of bacteraemia in acute leukaemia patients with severe neutropenia in a norwegian teaching hospital during the period 1990-95. gram negative bacteria represented 54% of the blood culture isolates, all of which were susceptible to aminoglycosides, and nearly all to ceftazidime and imipenem. penicillin/aminoglycoside was used as initial therapy in 43% of the episodes. initial empiric therapy was modified in 52% of the events. only 15% of ... | 1998 | 9889610 |
| antibiotic susceptibility among aerobic gram-negative bacilli in intensive care units in 5 european countries. french and portuguese icu study groups. | surveillance of antibiotic resistance is especially important in intensive care units (icus) because the infection rates are much higher there than in other hospital wards and most epidemics with multiresistant bacteria originate in icus. | 1999 | 9892453 |
| stenotrophomonas maltophilia endocarditis following dental treatment in a previously healthy patient. | 1998 | 9892541 | |
| a melanin pigment purified from an epidemic strain of burkholderia cepacia attenuates monocyte respiratory burst activity by scavenging superoxide anion. | the acquisition of burkholderia cepacia in some cystic fibrosis patients is associated with symptoms of acute pulmonary inflammation that may be life threatening. the ability of lipopolysaccharide (lps) from b. cepacia to prime a monocyte cell line for enhanced superoxide anion generation was investigated and compared with the priming activities of lpss from pseudomonas aeruginosa, stenotrophomonas maltophilia, and escherichia coli. the human monocyte cell line monomac-6 (mm6) was primed overnig ... | 1999 | 9916107 |
| aetiology, cost of antimicrobial therapy and outcome in neutropenic patients who developed bacteraemia during antimicrobial prophylaxis: a case-control study. | sixty four episodes of bacteraemia that appeared during antimicrobial prophylaxis with an oral quinolone plus an azole in neutropenic cancer patients were compared with 128 cases of bacteraemia in a cohort of controls matched for age, sex, underlying disease, neutropenia and vascular catheter in situ to assess differences in aetiology, cost of therapy and outcome. patients who received prophylaxis had breakthrough bacteraemias of a different aetiology compared with the control group: they had si ... | 1998 | 9916907 |
| [occurrence of gram-negative non-fermenting rods in hemocultures and their sensitivity to antimicrobial agents]. | in the period from january 1993 to june 1996 were at the department of microbiology of the university hospital in olomouc 122 strains of gram-negative nonfermentative rod-shaped bacteria isolated from haemocultures. the majority represented the group of 51 strains of the genus acinetobacter (41.8%), complex a. calcoaceticus-baumannii (acb complex). the second largest group were 21 strains (17.2%) of pseudomonas aeruginosa. these were followed by 17 strains (13.9%) of stenotrophomonas maltophilia ... | 1998 | 9919762 |
| kinetic mechanism of metallo-beta-lactamase l1 from stenotrophomonas maltophilia. | the reaction of nitrocefin with metallo-beta-lactamase l1 from stenotrophomonas maltophilia was studied using rapid-scan and stopped-flow ultraviolet-visible (uv-vis) studies in an effort to discern the kinetic mechanism used by l1 to hydrolyze penicillins and cephalosporins. rapid-scan and stopped-flow uv-vis studies of nitrocefin hydrolysis by l1 identified three species: (1) the substrate (nitrocefin) displayed an absorbance peak at 390 nm (epsilon = 11 500 m(-1) cm(-1)) that decreased during ... | 1999 | 9931021 |
| in vitro activity of meropenem against common pathogenic bacteria isolated in taiwan. | the in vitro antimicrobial activity of meropenem, in comparison with nine other antimicrobial agents, against 12 different common pathogenic bacteria were evaluated to know the susceptibility of common bacteria to meropenem in taiwan. meropenem was active against most gram-positive, gram-negative, and anaerobic bacteria, including methicillin-sensitive staphylococcus aureus, streptococcus pyogenes, enterococcus faecalis, escherichia coli, klebsiella pneumoniae, enterobacter cloacae, pseudomonas ... | 1998 | 9934544 |
| faucet aerators: a source of patient colonization with stenotrophomonas maltophilia. | multiple nosocomial outbreaks have been linked to contaminated water sources within the hospital. we report in this article a cluster of patients in a surgical intensive care unit who were colonized or infected with stenotrophomonas maltophilia. | 1999 | 9949380 |
| comparative in-vitro activities of trovafloxacin, ciproflaxacin, ofloxacin, and broad-spectrum beta-lactams against aerobe blood culture isolates. | the in vitro activity of trovafloxacin, a new fluoroquinolone, was compared with that of ciprofloxacin, ofloxacin, fleroxacin, ceftazidime, piperacillin/tazobactam, and meropenem against 613 consecutively recovered blood isolates from recently hospitalized patients. susceptibility testing was performed by agar dilution according to nccls guidelines. test strains included acinetobacter species (n = 26), escherichia coli (n = 137), enterobacter species (n = 27), klebsiella species (n = 42), proteu ... | 1998 | 9987189 |
| in vitro activity of trovafloxacin against ciprofloxacin-susceptible and -resistant clinical bacterial isolates and assessment of the trovafloxacin disk test. | a total of 4241 consecutive clinical bacterial isolates from 10 north american medical centers were tested for susceptibility to trovafloxacin. trovafloxacin was significantly more active than ciprofloxacin against gram-positive bacteria, acinetobacter spp., and stenotrophomonas maltophilia, and resistance to trovafloxacin occurred in these groups only among isolates with high-level resistance (mic > or = 16 micrograms/ml) to ciprofloxacin. with other species, the two drugs had comparable activi ... | 1999 | 9990473 |
| lipopolysaccharide (lps) from burkholderia cepacia is more active than lps from pseudomonas aeruginosa and stenotrophomonas maltophilia in stimulating tumor necrosis factor alpha from human monocytes. | whole cells and lipopolysaccharides (lpss) extracted from burkholderia cepacia, pseudomonas aeruginosa, stenotrophomonas maltophilia, and escherichia coli were compared in their ability to stimulate tumor necrosis factor alpha (tnf-alpha) from the human monocyte cell line monomac-6. b. cepacia lps, on a weight-for-weight basis, was found to have tnf-alpha-inducing activity similar to that of lps from e. coli, which was approximately four- and eightfold greater than the activity of lpss from p. a ... | 1999 | 10024601 |
| [evaluation of mycobacterium kansasii isolates from a clinical laboratory in a city hospital]. | fifteen isolates of mycobacterium kansasii in showa university fujigaoka hospital between 1982 and 1995 were investigated. comparing by gender, 13 were isolated from male patients and only two were isolated from female patients. the average of cases was 48 years old and 14 out of 15 cases (93%) were isolated from respiratory tract specimens. the rate of the smear- and culture-positives was 64%, which was significantly higher than that (26%) of m. avium complex (p < 0.01 by chi 2 test). all 4 iso ... | 1999 | 10067052 |
| stenotrophomonas maltophilia infection and colonization in the intensive care units of two community hospitals: a study of 143 patients. | study objective: to study the epidemiology of stenotrophomonas maltophilia infections in the intensive care units (icus) of community general hospitals. | 1999 | 10076113 |
| the increase in carbapenem use and emergence of stenotrophomonas maltophilia as an important nosocomial pathogen. | carbapenems, being the broadest spectrum antibiotics, may allow those organisms intrinsically resistant to these drugs to be involved more frequently in nosocomial infections. imipenem was introduced to the specialized hospital units in kuwait in october, 1992 and meropenem in late 1996. the main objective of this study was to observe prospectively whether, under similar laboratory conditions for microbial isolation/identification while these drugs are in use in the hospital, there is any propor ... | 1999 | 10078777 |
| [clinical manifestations of stenotrophomas (xanthomonas) maltophilia infection]. | stenotrophomonas maltophilia (sm) is a gram-negative bacillus whose incidence like nosocomial pathogen has been incremented in the last years, especially in immunocompromised patients, subjected to invasive procedures and those receiving broad-spectrum antimicrobial therapy. | 1998 | 10079538 |
| [xanthomonas maltophilia pneumonia]. | 1999 | 10088424 | |
| in vitro activity of ciprofloxacin, levofloxacin, and trovafloxacin, alone and in combination with beta-lactams, against clinical isolates of pseudomonas aeruginosa, stenotrophomonas maltophilia, and burkholderia cepacia. | we tested three fluoroquinolones (ciprofloxacin, levofloxacin, and trovafloxacin), each combined with each of four beta-lactams (cefoperazone, ceftriaxone, imipenem, and meropenem) for synergy against clinical isolates of nosocomial strains of pseudomonas aeruginosa, stenotrophomonas maltophilia, and burkholderia cepacia. the ciprofloxacin-beta-lactam combinations showed synergy against none or only a small fraction (7 to 10%) of the p. aeruginosa and b. cepacia isolates. ciprofloxacin-cefoperaz ... | 1999 | 10091030 |
| surfactant-enhanced biodegradation of high molecular weight polycyclic aromatic hydrocarbons by stenotrophomonas maltophilia | the objectives of this study were to isolate and evaluate microorganisms with the ability to degrade high molecular weight polycyclic aromatic hydrocarbons (pahs) in the presence of synthetic surfactants. stenotrophomonas maltophilia vun 10,010, isolated from pah-contaminated soil, utilized pyrene as a sole carbon and energy source and also degraded other high molecular weight pahs containing up to seven benzene rings. various synthetic surfactants were tested for their ability to improve the pa ... | 1998 | 10099362 |
| location and survival of leaf-associated bacteria in relation to pathogenicity and potential for growth within the leaf | the growth and survival of pathogenic and nonpathogenic pseudomonas syringae strains and of the nonpathogenic species pantoea agglomerans, stenotrophomonas maltophilia, and methylobacterium organophilum were compared in the phyllosphere of bean. in general, the plant pathogens survived better than the nonpathogens on leaves under environmental stress. the sizes of the total leaf-associated populations of the pathogenic p. syringae strains were greater than the sizes of the total leaf-associated ... | 1999 | 10103233 |
| effect of chronic intermittent administration of inhaled tobramycin on respiratory microbial flora in patients with cystic fibrosis. | pseudomonas aeruginosa endobronchial infection causes significant morbidity and mortality among cystic fibrosis patients. microbiology results from two multicenter, double-blind, placebo-controlled trials of inhaled tobramycin in cystic fibrosis were monitored for longitudinal changes in sputum microbial flora, antibiotic susceptibility, and selection of p. aeruginosa isolates with decreased tobramycin susceptibility. clinical response was examined to determine whether current susceptibility sta ... | 1999 | 10191222 |
| stenotrophomonas (xanthomonas) maltophilia infection in necrotizing pancreatitis. | although the therapy of infected pancreatic collections or organized pancreatic necrosis remains surgical, we have demonstrated that infected organized pancreatic necrosis can be treated endoscopically. | 1999 | 10211423 |
| carbapenems and monobactams: imipenem, meropenem, and aztreonam. | imipenem and meropenem, members of the carbapenem class of beta-lactam antibiotics, are among the most broadly active antibiotics available for systemic use in humans. they are active against streptococci, methicillin-sensitive staphylococci, neisseria, haemophilus, anaerobes, and the common aerobic gram-negative nosocomial pathogens including pseudomonas. resistance to imipenem and meropenem may emerge during treatment of p. aeruginosa infections, as has occurred with other beta-lactam agents; ... | 1999 | 10221472 |
| important aspects of the colonization of central venous catheter. | this study comprises five different kind of venous central catheters, 103 in total, made of polyurethane tecoflex, polyurethane vialon, ptfe and pvc, and the influence of their raw material on the microbial colonization. patients age and sex, besides their clinical conditions, were taken into account, and neither considered as a sample vicious, nor associated with colonization. when the tips of the catheters were asseptically inoculated in tryptic soy broth and tioglicolate, colonization was det ... | 1999 | 10326311 |
| in vitro activities of designed antimicrobial peptides against multidrug-resistant cystic fibrosis pathogens. | the emergence of multidrug-resistant pathogens renders antibiotics ineffective in the treatment of lung infections in patients with cystic fibrosis (cf). designed antimicrobial peptides (daps) are laboratory-synthesized peptide antibiotics that demonstrate a wide spectrum of antibacterial activity. optimal conditions for susceptibility testing of these peptides have not yet been established. medium composition is clearly a major factor influencing the results and reproducibilities of susceptibil ... | 1999 | 10348766 |
| correlation between genotype and beta-lactamases of clinical and environmental strains of stenotrophomonas maltophilia. | heterogeneity of beta-lactamase production by 17 clinical and nine environmental isolates of stenotrophomonas maltophilia was investigated using mics of six different beta-lactam antibiotics, isoelectric focusing (ief) and pulsed field gel electrophoresis. there was no clear correlation between the results of ief, genotype and mic determination. environmental isolates were more susceptible than clinical isolates; eight clinical and none of the environmental isolates expressed high-level resistan ... | 1999 | 10350386 |
| identification of burkholderia spp. in the clinical microbiology laboratory: comparison of conventional and molecular methods. | cystic fibrosis (cf) predisposes patients to bacterial colonization and infection of the lower airways. several species belonging to the genus burkholderia are potential cf-related pathogens, but microbiological identification may be complicated. this situation is not in the least due to the poorly defined taxonomic status of these bacteria, and further validation of the available diagnostic assays is required. a total of 114 geographically diverse bacterial isolates, previously identified in re ... | 1999 | 10364579 |
| a highly efficient and convergent synthesis of a hexasaccharide, a dimer of the repeating unit of the antigen o2 polysaccharide of stenotrophomonas maltophilia. | a highly efficient and convergent synthesis of a hexasaccharide, which is a dimer of the repeating unit of the antigen o2 polysaccharide of stenotrophomonas maltophilia, was achieved via coupling of 2,3,4-tri-o-acetyl-alpha-l-xylopyranosyl bromide with the tetrasaccharide, allyl 4-o-{3-o-[4-o-(3,4-di-o-benzoyl-alpha-l-rhamnopyranosyl)-2,3,6-tri-o-ben zoyl -alpha-d-mannopyranosyl]-4-benzoyl-alpha-l-rhamnopyranosyl}-2,3,6-tri-o- benzoyl-alpha-d-mannopyranoside (18) by the koenigs-knorr method foll ... | 1999 | 10385975 |
| identification of a novel beta-lactamase produced by xanthomonas campestris, a phytopathogenic bacterium. | the xanthomonas campestris pv. campestris 11 chromosome encodes a periplasmic beta-lactamase of 30 kda. gene replacement and complementation confirmed the presence of this enzyme. its deduced amino acid sequence shows identity and conserved domains between it and stenotrophomonas maltophilia l2 and other ambler class a/bush group 2 beta-lactamases. southern hybridization detected a single homologous fragment in each of 12 other xanthomonas strains, indicating that the presence of a beta-lactamas ... | 1999 | 10390247 |
| characterization of is1389, a new member of the is3 family of insertion sequences isolated from xanthomonas campestris pv. amaranthicola. | is1389, a new insertion sequence belonging to the is3 family, has been identified in xanthomonas campestris pv. amaranthicola. the genome of this bacterium contains at least 11 copies of the element, whereas no hybridizing sequences were detected in other xanthomonas species [x. axonopodis, x. fragaridae, x. phaseoli, and x. (stenotrophomonas) maltophila]. two nearly identical copies of the element (is1389-a and is1389-b) were characterized. according to analysis of sequence alignments and simil ... | 1999 | 10398747 |
| susceptibility to levofloxacin of clinical isolates of bacteria from intensive care and haematology/oncology patients in switzerland: a multicentre study. | the objective of this study was to examine the susceptibility of clinical isolates to levofloxacin, a fluoroquinolone with extended activity against gram-positive bacteria, and other antibiotics in 12 swiss clinical microbiology laboratories using the nccls disc diffusion technique. isolates were prospectively collected from intensive care units (icus (59%), oncology wards (7%) and other units with haematology/oncology patients (34%) from june 1995 to march 1996. the levofloxacin breakpoints use ... | 1999 | 10404338 |
| inhibition studies on the metallo-beta-lactamase l1 from stenotrophomonas maltophilia. | in an effort to identify a competitive inhibitor that can be used in future spectroscopic and crystallographic studies and to better understand the interaction of a mercaptoacetic acid-thiolester-containing compound with metallo-beta-lactamase l1 from stenotrophomonas maltophilia, inhibition studies using two thiol-containing compounds were conducted. n-(2'-mercaptoethyl)-2-phenylacetamide is a competitive inhibitor of l1 with a k(i) of 50 +/- 3 microm, and this compound is not a time-dependent ... | 1999 | 10415104 |
| peritonitis due to stenotrophomonas maltophilia in patients undergoing chronic peritoneal dialysis. | the occurrence of cases of stenotrophomonas maltophilia peritonitis in chronic peritoneal dialysis (pd) patients prompted a review of our experience with this condition. a search of microbiology records revealed seven episodes of s. maltophilia peritonitis in 7 patients in 1996 - 3.8% of all pd patients - compared to no cases in 1994 and 1995 (p = 0.01). patients ranged in age from 16 to 64 years; there were 3 males and 4 females. six of seven episodes of peritonitis were community acquired and ... | 1999 | 10433163 |
| [non fermentative gram negative bacilli isolated in a hospital laboratory]. | to report a prospective study on non fermentative gramnegative bacilli, excluded pseudomonas aeruginosa, isolated at dr. julio c. perrando hospital in resistencia (argentina). the goal of this study was to know their frequency and antimicrobial susceptibility. | 1999 | 10439535 |
| pseudoaneurysm of the subclavian artery due to xanthomonas pneumonia in a patient with acute myeloid leukemia: its rupture treated by transcatheter coil embolization. | a 52-year-old male with acute myeloid leukemia developed pseudoaneurysm of the subclavian artery. pneumonia due to xanthomonas maltophilia, which was multi-drug resistant, progressed to a lung abscess even under administration of antibiotics. this lung infection contiguous to the left carotid and subclavian arteries was suggested to have caused the pseudoaneurysm of the subclavian artery. the rupture of the aneurysm by penetration to the trachea amounted to about 1,000 ml of bleeding; fortunatel ... | 1999 | 10440506 |
| comparative in vitro activity of gatifloxacin against stenotrophomonas maltophilia and burkholderia species isolates including evaluation of disk diffusion and e test methods. | the in vitro activity of gatifloxacin, a new fluoroquinolone, was compared to that of five other fluoroquinolones against 105 stenotrophomonas maltophilia isolates and 52 burkholderia spp. isolates. the gatifloxacin mics were determined using the broth microdilution method and the e test (ab biodisk, sweden); these methods were compared for test accuracy, and 5 microg disk zone diameters were compared for interpretive accuracy using the standardized disk diffusion method. in terms of potency, ga ... | 1999 | 10442421 |
| histamine and cadaverine production by bacteria isolated from fresh and frozen albacore (thunnus alalunga). | two hundred twenty-seven bacterial strains were isolated from fresh and frozen albacore stored either at -18 or -25 degrees c and investigated for their abilities to produce biogenic amines. as a preliminary screening, all 227 strains were tested in either niven or niven modified medium, which allowed the selection of 25 presumptive histamine-producing strains. high-pressure liquid chromatography revealed that only 10 of the 25 strains selected were able to produce low histamine concentrations ( ... | 1999 | 10456749 |
| two consecutive outbreaks of stenotrophomonas maltophilia (xanthomonas maltophilia) in an intensive-care unit defined by restriction fragment-length polymorphism typing. | to investigate and control consecutive outbreaks of stenotrophomonas maltophilia infections in intensive-care-unit (icu) patients. | 1999 | 10466556 |
| in vitro activity of newer broad spectrum beta-lactam antibiotics against enterobacteriaceae and non-fermenters: a report from austrian intensive care units. austrian carbapenem susceptibility surveillance group. | we compared the in vitro activity of broad spectrum beta-lactam antibiotics against 573 gram-negative isolates (enterobacteriaceae and non-fermenters) collected between november 1996 and may 1997 from 9 laboratories serving intensive care units throughout austria. mic's (minimal inhibitory concentration) were obtained with the e-test for meropenem, imipenem, ceftazidime, cefepime, cefpirome and piperacillin/tazobactam. pseudomonas aeruginosa was the most frequently isolated organism (22%), follo ... | 1999 | 10467641 |
| development of rrna-based pcr assays for identification of burkholderia cepacia complex isolates recovered from cystic fibrosis patients. | pcr assays targeting rrna genes were developed to identify species (genomovars) within the burkholderia cepacia complex. each assay was tested with 177 bacterial isolates that also underwent taxonomic analysis by whole-cell protein profile. these isolates were from clinical and environmental sources and included 107 b. cepacia complex strains, 23 burkholderia gladioli strains, 20 ralstonia pickettii strains, 10 pseudomonas aeruginosa strains, 8 stenotrophomonas maltophilia strains, and 9 isolate ... | 1999 | 10488171 |
| capillary electrophoresis-single-strand conformation polymorphism analysis for rapid identification of pseudomonas aeruginosa and other gram-negative nonfermenting bacilli recovered from patients with cystic fibrosis. | we used capillary electrophoresis-single-strand conformation polymorphism (ce-sscp) analysis of pcr-amplified 16s rrna gene fragments for rapid identification of pseudomonas aeruginosa and other gram-negative nonfermenting bacilli isolated from patients with cystic fibrosis (cf). target sequences were amplified by using forward and reverse primers labeled with various fluorescent dyes. the labeled pcr products were denatured by heating and separated by capillary gel electrophoresis with an autom ... | 1999 | 10488211 |