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diversity change of microbial communities responding to zinc and arsenic pollution in a river of northeastern china.pollution discharge disturbs the natural functions of water systems. the environmental microbial community composition and diversity are sensitive key indicators to the impact of water pollutant on the microbial ecology system over time. it is meaningful to develop a way to identify the microbial diversity related to heavy metal effects in evaluating river pollution. water and sediment samples were collected from eight sections along the tiaozi river where wastewater and sewage were discharged f ...201425001226
to invade or not to invade: two approaches to a prokaryotic predatory life cycle.bdellovibrio and like organisms (balos) are a group of gram-negative bacterial predators that are defined as having a periplasmic life cycle, whereby the predator enters into the periplasm of a prey cell. recently, a predator of caulobacter crescentus with a novel epibiotic life cycle was identified as a new species - bdellovibrio exovorus. therefore, this raises the question as to what determines the type of life cycle of a predator. six bacterial strains susceptible to predation by b. exovorus ...201323586752
deciphering mediating characteristics of decolorized intermediates for reductive decolorization and bioelectricity generation.as decolorized intermediates could play a role of electron-shuttling mediator to enhance the performance of dye decolorization and bioelectricity generation, this study selected model compounds with auxochromes (e.g., benzene-1,2-diol, 1,2-diaminobenzene) to explore how chemical structure(s) affected color removal and power producing capabilities in microbial fuel cells (mfcs). according to cyclic voltammetry, respiratory testing and mfc data, promising electron-shuttling capabilities of aforeme ...201323385155
blandm-1-carrying acinetobacter johnsonii detected in hospital sewage.this study screened hospital sewage for the presence of blandm.201323288403
inorganic impurity removal from waste oil and wash-down water by acinetobacter johnsonii.the removal of the abundant inorganic impurities in waste oil has been one of the most significant issues in waste oil reclamation. acinetobacter johnsonii isolated from waste oil in aerobic process was employed to remove the inorganic impurities in waste oil and wash-down water. the biological process was developed through the primary mechanism research on the impurity removal and the optimization of the various parameters, such as inoculum type, inoculum volume and disposal temperature and tim ...201223021100
mosquito microbiome dynamics, a background for prevalence and seasonality of west nile virus.symbiotic microbial communities augment host phenotype, including defense against pathogen carriage and infection. we sampled the microbial communities in 11 adult mosquito host species from six regions in southern ontario, canada over 3 years. of the factors examined, we found that mosquito species was the largest driver of the microbiota, with remarkable phylosymbiosis between host and microbiota. seasonal shifts of the microbiome were consistently repeated over the 3-year period, while region ...201728421042
determination of spoilage microbiota of pacific white shrimp during ambient and cold storage using next-generation sequencing and culture-dependent method.this study was conducted to determine the initial and spoilage microbiota of pacific white shrimp during ambient and cold storage using next-generation sequencing (ngs) and a culture-dependent method. the quality changes were also evaluated by sensory analysis and total volatile basic nitrogen (tvb-n) values. after 1 d of storage, the psychrotrophic bacteria were only 5.97 log cfu/g, accounting for 1.1% of the mesophilic bacteria counts (7.94 log cfu/g). the psychrotrophic bacteria counts exceed ...201728399332
acinetobacter kookii sp. nov., isolated from soil.two gram-stain-negative, non-fermentative bacterial strains, designated 11-0202(t) and 11-0607, were isolated from soil in south korea, and four others, luh 13522, luh 8638, luh 10268 and luh 10288, were isolated from a beet field in germany, soil in the netherlands, and sediment of integrated fish farms in malaysia and thailand, respectively. based on 16s rrna, rpob and gyrb gene sequences, they are considered to represent a novel species of the genus acinetobacter. their 16s rrna gene sequence ...201323950148
induction of diverse bioactive secondary metabolites from the mangrove endophytic fungus trichoderma sp. (strain 307) by co-cultivation with acinetobacter johnsonii (strain b2).two new sesquiterpenes, microsphaeropsisin b (1) and c (2), and two new de-o-methyllasiodiplodins, (3r, 7r)-7-hydroxy-de-o-methyllasiodiplodin (4) and (3r)-5-oxo-de-o-methyllasiodiplodin (5), together with one new natural product (6) and twelve known compounds (3, 7-17), were isolated from the co-cultivation of mangrove endophytic fungus trichoderma sp. 307 and aquatic pathogenic bacterium acinetobacter johnsonii b2. their structures, including absolute configurations, were elucidated by extensi ...201728208607
the influence of the autochthonous wastewater microbiota and gene host on the fate of invasive antibiotic resistance genes.the aim of this study was to assess the fate of invasive antibiotic resistance genes (arg) discharged in wastewater. with this objective, antibiotic resistant bacteria (arb) known to harbor specific arg were inoculated in wastewater (hospital effluent, or municipal raw and treated wastewater) and in ultra-pure sterile water microcosms. two sets of wastewater arb isolates were used - set 1, enterococcus faecalis, acinetobacter johnsonii, klebsiella pneumoniae and set 2, enterococcus faecium, acin ...201727697350
diurnal variation in bacterioplankton composition and dna damage in the microbial community from an andean oligotrophic lake.laguna azul is an oligotrophic lake situated at 4,560 m above sea level and subject to a high level of solar radiation. bacterioplankton community composition (bcc) was analysed by denaturing gradient gel electrophoresis and the impact of solar ultraviolet radiation was assessed by measuring cyclobutane pyrimidine dimers (cpd). furthermore, pure cultures of acinetobacter johnsonii a2 and rhodococcus sp. a5 were exposed simultaneously and cpd accumulation was studied. gel analyses generated a tot ...201525576421
first isolation of acinetobacter johnsonii co-producing per-2 and oxa-58 β-lactamases. 201425294301
evaluation of bacterial pathogen diversity, abundance and health risks in urban recreational water by amplicon next-generation sequencing and quantitative pcr.the microbial quality of urban recreational water is of great concern to public health. the monitoring of indicator organisms and several pathogens alone is not sufficient to accurately and comprehensively identify microbial risks. to assess the levels of bacterial pathogens and health risks in urban recreational water, we analyzed pathogen diversity and quantified four pathogens in 46 water samples collected from waterbodies in beijing olympic forest park in one year. the pathogen diversity rev ...201728647233
rna-based amplicon sequencing reveals microbiota development during ripening of artisanal versus industrial lard d'arnad.valle d'aosta lard d'arnad is a protected designation of origin (pdo) product produced from fat of the shoulder and back of heavy pigs. its manufacturing process can be very diverse, especially regarding the maturation temperature and the nacl concentration used for the brine; thereby, the main goal of this study was to investigate the impact of those parameters on the microbiota developed during curing and ripening. three farms producing lard d'arnad were selected. two plants, reflecting the in ...201728600315
using phenotype microarrays in the assessment of the antibiotic susceptibility profile of bacteria isolated from wastewater in on-site treatment facilities.the scope of the study was to apply phenotype biolog microarray (pm) technology to test the antibiotic sensitivity of the bacterial strains isolated from on-site wastewater treatment facilities. in the first step of the study, the percentage values of resistant bacteria from total heterotrophic bacteria growing on solid media supplemented with various antibiotics were determined. in the untreated wastewater, the average shares of kanamycin-, streptomycin-, and tetracycline-resistant bacteria wer ...201728451946
draft genome sequence of acinetobacter johnsonii c6, an environmental isolate engaging in interspecific metabolic interactions.acinetobacter johnsonii c6 originates from creosote-polluted groundwater and performs ecological and evolutionary interactions with pseudomonas putida in biofilms. the draft genome of a. johnsonii c6 is 3.7 mbp and was shaped by mobile genetic elements. it reveals genes facilitating the biodegradation of aromatic hydrocarbons and resistance to antimicrobials and metals.201728428290
a topical hydrogel with deferiprone and gallium-protoporphyrin targets bacterial iron metabolism and has antibiofilm activity.many infectious diseases are associated with multidrug-resistant (mdr) bacteria residing in biofilms that require high antibiotic concentrations. while oral drug delivery is frequently ineffective, topical treatments have the potential to deliver higher drug concentrations to the infection site while reducing systemic side effects. this study determined the antibiofilm activity of a surgical wound gel loaded with the iron chelator deferiprone (def) and the heme analogue gallium-protoporphyrin (g ...201728396543
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