Publications

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intestinal nematodes from small mammals captured near the demilitarized zone, gyeonggi province, republic of korea.a total of 1,708 small mammals (1,617 rodents and 91 soricomorphs), including apodemus agrarius (n = 1,400), microtus fortis (167), crocidura lasiura (91), mus musculus (32), myodes (= eothenomys) regulus (9), micromys minutus (6), and tscherskia (= cricetulus) triton (3), were live-trapped at us/republic of korea (rok) military training sites near the demilitarized zone (dmz) of paju, pocheon, and yeoncheon, gyeonggi province from december 2004 to december 2009. small mammals were examined for ...201525748722
[risk assessments and control strategies of plague in five key surveillance counties, zhejiang province].to analyze the epidemiology data on plague in five counties in zhejiang province and to evaluate the risk of plague in theses areas.201526813723
subclinical tick-borne encephalitis virus in experimentally infected apodemus agrarius.in this study, we investigated the dose dependence of tick-borne encephalitis virus (tbev) infection in one of the reservoirs, i.e. apodemus agrarius, a small rodent species.201526934202
bank voles show high seropositivity rates in a natural tbev focus in hungary.rodents captured in a known tick-borne encephalitis virus (tbev) focus were serologically surveyed for 4 years, with 28 visits. the collected sera were analysed by virus neutralization test. bank vole (myodes glareolus) had a significantly higher incidence rate of antibodies to tbev (20.5%) than apodemus flavicollis (3.7%) and apodemus agrarius (4.6%). in all species, rates were higher in adults (6.8%) than in juveniles (1.7%). a higher incidence rate was observed in female a. flavicollis indivi ...201525549698
detection and molecular characterization of cryptosporidium spp. from wild rodents and insectivores in south korea.in order to examine the prevalence of cryptosporidium infection in wild rodents and insectivores of south korea and to assess their potential role as a source of human cryptosporidiosis, a total of 199 wild rodents and insectivore specimens were collected from 10 regions of south korea and screened for cryptosporidium infection over a period of 2 years (2012-2013). a nested-pcr amplification of cryptosporidium oocyst wall protein (cowp) gene fragment revealed an overall prevalence of 34.2% (68/1 ...201526797442
host-dependent morphology of isthmiophora melis (schrank, 1788) luhe, 1909 (digenea, echinostomatinae)--morphological variation vs. molecular stability.echinostomes are cosmopolitan digenean parasites which infect many different warm-blooded hosts. their classification is extremely confused; the host spectrum is wide, and morphological similarities often result in misidentification. during our long-term studies on the helminth fauna of rodents and carnivores we have collected 27 collar-spined echinostomes which differ in morphology to an extent that suggests the presence of more than one species. here, we describe this material, and the extent ...201526394689
apodemus agrarius is a potential natural host of severe fever with thrombocytopenia syndrome (sfts)-causing novel bunyavirus.severe fever with thrombocytopenia syndrome (sfts) is a tick-borne, novel bunyavirus-caused emerging infectious disease. it becomes a threat to public health due to its high fatality rate.201526322483
genetic diversity and genetic structure of the striped field mouse apodemus agrarius coreae (muridae, rodentia) in korea.the aim of this study was to investigate the genetic diversity and genetic structure of the striped field mouse apodemus agrarius coreae in korea. the korean a. a. coreae is characterized by high levels of haplotype diversity (hd=0.967) and low levels of nucleotide diversity (π=0.00683). haplogroup 1 is well separated from the haplotypes of the neighboring regions of the korean peninsula, while the other haplogroups are closely related to those from the russian far east. thus, further investigat ...201526263836
[population change of farmland rodent and the influences of climate and cultivation factors in fengxian district of shanghai, china].we analyzed the changes of rodent population from 1988 to 2013 in fengxian district, shanghai, and discussed the relevance of rodent population dynamics and climate, and ecological factors. results showed that the average rat density was 1.3% from 1988 to 2013, and the dominant species was apodemus agrarius. there was little change of density between years. the average composition ratio was 97.5%. the peak of rodent density appeared from february to may, highest in march. average density and pea ...201526094477
[criteria of difference evaluation for-various types of hemorrhagic fever with renal syndrome foci. hfrs foci in various biotopes of northern barrens].determine qualitative and quantitative criteria of evaluation of features for natural, natural-anthropourgic and anthropourgic foci of hantaviruses (using hemorrhagic fever with renal syndrome--hfrs foci as an example), that have formed in various areas of saratov region.201526016349
sex differences in flea infections among rodent hosts: is there a male bias?recognizing patterns of parasite distribution among wildlife hosts is of major importance due to growing risk of transmission of zoonotic diseases to humans. thus, sex-dependent parasite distribution in higher vertebrates is extensively studied, and males are often found more parasitized than females. male-biased parasitism may be the result of weaker immunocompetence of male hosts owing to the immunosuppressive effect of androgens. moreover, larger hosts (males) may demonstrate higher parasite ...201525410932
host-associated differences in morphometric traits of parasitic larvae hirsutiella zachvatkini (actinotrichida: trombiculidae).examination of host-associated variation in the chigger mite hirsutiella zachvatkini (schluger) revealed morphological differences among larvae infesting sympatric hosts: apodemus agrarius, apodemus flavicollis and myodes glareolus. the analysis included 61 variables of larvae obtained from their gnathosoma, idiosoma and legs (measurements and counts). statistically significant differences were observed for metric characters of the legs as opposed to the scutum. in view of the conspecificity of ...201526002309
small rodents as reservoirs of cryptosporidium spp. and giardia spp. in south-western poland.cryptosporidium spp. and giardia spp. have been detected in a range of host species, including rodents. the aim of this study was to determine the distribution of these pathogens and recognition of the reservoir role of rodents in the maintenance of these pathogens in south-western poland. additionally, preliminary molecular studies were conducted to elucidate the species and genotypes of cryptosporidium and giardia identified in this study. stool samples (n=266) from a. agrarius, a. flavicollis ...201525780818
autochthonous dobrava-belgrade virus infection in eastern germany.a 21-year-old male patient from borna, saxony, in eastern germany, suffered from acute kidney injury (aki) and symptoms typical for a hantavirus infection. these symptoms included nausea, vomiting, abdominal pain, diarrhea, and acute renal failure. serological investigations by indirect igm and igg in-house elisas, commercial immunofluorescence and line assays, as well as chemiluminescence focus reduction neutralization assay confirmed an acute dobrava-belgrade virus (dobv) infection of the pati ...201524495905
genetic detection of dobrava-belgrade hantavirus in the edible dormouse (glis glis) in central serbia.hantaviruses are endemic in the balkans, particularly in serbia, where sporadic cases and/or outbreaks of hantaviral human disease have been reported repeatedly, and evidenced serologically. here, we present genetic detection of dobrava-belgrade virus (dobv) hantaviral sequences in wild rodents trapped in central serbia. all the animals were pre-screened serologically by indirect immunofluorescence (if) test and only those with a positive finding of hantaviral antigens were further tested by pol ...201524762257
serologic survey of orthopoxvirus infection among rodents in hungary.as a result of discontinuing vaccination against smallpox after the late 1970s, different orthopoxviruses (opvs), such as cowpox virus (cpxv), have become a re-emerging healthcare threat among zoonotic pathogens. in hungary, data on opv prevalence among its rodent host species have been absent. here, rodents belonging to four species, i.e., striped field mouse (apodemus agrarius), yellow-necked mouse (a. flavicollis), wood mouse (a. sylvaticus) and bank vole (myodes glareolus), were live trapped ...201525988441
rickettsia species in fleas collected from small mammals in slovakia.epidemiological and epizootiological studies of rickettsia felis and other rickettsia spp. are very important, because their natural cycle has not yet been established completely. in total, 315 fleas (siphonaptera) of 11 species of ceratophyllidae, hystrichopsyllidae and leptopsyllidae families were tested for the presence of rickettsia species and coxiella burnetii with conventional and specific quantitative real-time pcr assays. fleas were collected from five rodent hosts (myodes glareolus, ap ...201526346455
candidatus neoehrlichia mikurensis and anaplasma phagocytophilum in natural rodent and tick communities in southern hungary.the aim of this study was to investigate the natural cycle of the new human pathogenic bacteria candidatus neoehrlichia mikurensis and anaplasma phagocytophilum in southern hungary. we collected rodents with live-traps (2010-2013) and questing ticks with flagging in 2012. small mammals were euthanized, tissue samples were collected and all the ectoparasites were removed and stored in 70% alcohol. we found relatively low overall prevalence of tick infestation (8%). samples were analysed for a. ph ...201525468763
molecular examinations of babesia microti in rodents and rodent-attached ticks from urban and sylvatic habitats in germany.small mammals serve as reservoir hosts for tick-borne pathogens, especially for those which are not transmitted transovarially in ticks - such as babesia microti. molecular investigations on the prevalence of b. microti in wild small mammals and on attached ticks from differently structured areas may provide information on the circulation of b. microti in different ecological niches. in 2012 and 2013, 622 rodents (396 myodes glareolus, 178 apodemus flavicollis, 36 apodemus sylvaticus, 4 apodemus ...201525922232
molecular survey of zoonotic agents in rodents and other small mammals in croatia.croatia is a focus for many rodent-borne zoonosis. here, we report a survey of 242 rodents and small mammals, including 43 myodes glareolus, 131 apodemus flavicollis, 53 apodemus agrarius, three apodemus sylvaticus, six sorex araneus, four microtus arvalis, one microtus agrestis, and one muscardinus avellanarius, collected at eight sites in croatia over an 8-year period. multiplex masstag polymerase chain reaction (pcr) was used for detection of borrelia, rickettsia, bartonella, babesia, ehrlich ...201626711522
presence of candidatus neoehrlichia mikurensis and babesia microti in rodents and two tick species (ixodes ricinus and ixodes trianguliceps) in slovakia.rodents are important reservoir hosts of many tick-borne pathogens. their importance in the circulation of the emerging bacterial agent, candidatus neoehrlichia mikurensis and the intraerythrocytic protozoan parasite, babesia microti has been recently proposed. the aim of the present study was to identify the presence and genetic diversity of candidatus n. mikurensis and b. microti circulating in the natural foci among rodents and two species of ixodid ticks (ixodes ricinus and ixodes triangulic ...201626700195
prevalence, isolation and molecular characterization of bartonella species in republic of korea.to determine the prevalence of bartonella species and identify which species of bartonella naturally infects the striped field mouse (apodemus agrarius) in the republic of korea (rok), spleens from 200 mice were assayed by nested polymerase chain reaction (npcr) targeting the rna polymerase subunit beta (rpob) gene and the 16s-23s internal transcribed spacer (its) region for members of the genus bartonella. utilizing pcr techniques, the prevalence of bartonella spp. ranged from 31.5% (63/200) to ...201624661833
[diversity of the mitochondrial dna cytochrome b gene of the field mouse apodemus agrarius pallas, 1771 in the south of the russian far east].we studied the variability of a fragment of the cytochrome b gene and the corresponding amino acid sequence of the field mouse apodemus agrarius (pallas, 1771) in the south of primorskii krai (far east of russia). a significant polymorphism of these traits was detected. high indices of molecular diversity were obtained. the topology of the phylogenetic tree and the median networks suggest that the population of field mice in the south of primorskii krai originated from three maternal lines. it w ...201625735151
phylogenetic analysis of the complete mitochondrial genome of the korean field mouse apodemus peninsulae (rodentia, murinae) from china.we sequenced and characterized a complete mitogenome (kp671850) of the chinese apodemus peninsulae and compared it with a previously published mitogenome of the korean a. peninsulae (nc016060). the total length of the chinese a. peninsulae mitogenome is 16,457 bp. the mitogenome consists of 13 protein-coding genes (pcgs), two rrna (12s rrna and 16s rrna) genes, 22 trna genes, and one d-loop region. the most common start codon was atg, used in the nine pcgs for initiation. the mitogenomes of chin ...201626006285
geographical distribution and seasonal indices of chigger mites on small mammals collected on the east coast of the republic of korea.the geographical distributions and relative population densities of scrub typhus vector mites collected from small mammals were determined for 5 locations on the east coast of the republic of korea. collection sites included goseong, gangneung, and hoengseong in gangwon province and uljin and yeongdeok in gyeongbuk province. a total of 275 small mammals including members of rodentia (rodents) and soricomorpha (shrews, such as crocidura lasiura ) belonging to 4 genera and 4 species were captured ...201626653927
genetic diversity of bartonella genotypes found in the striped field mouse (apodemus agrarius) in central europe.we investigated the diversity of bartonella in apodemus agrarius, an important rodent of peri-domestic habitats, which has spread into europe in the past 1000 years. spleen samples of 344 a. agrarius from eastern slovakia were screened for the presence of bartonella spp. using 16s-23s rrna internal transcribed spacer region and bacteria were detected in 9% of rodents. based on sequencing of three housekeeping genes (glta, rpob and groel) bartonella genotypes were ascribed to the species typical ...201627279125
[optimization of elisa and immunoblot methods for the detection of igg antibodies against old world hantaviruses in wild rodents].hantaviruses infect humans via inhalation of viral particles in infected rodents' secretions such as saliva, urine and faeces or via direct contact with infected rodents. the rodent species that are known as the carriers of dobrava (dobv), puumala (puuv), saaremaa (saav), tula (tulv) and seoul (seov) viruses are found in our country. the presence of specific antibodies against hantaviruses have been demonstrated in rodents collected from black sea and aegean regions of turkey in 2004 for the fir ...201627175497
identification of hepatozoon erhardovae krampitz, 1964 from bank voles (myodes glareolus) and fleas in southern hungary.in order to investigate the prevalence and life cycle of apicomplexan parasites, small mammals were live-trapped with modified sherman traps in southern hungary between 2010 and 2012. altogether, 528 rodents (apodemus flavicollis melchior, 1834, apodemus agrarius pallas, 1771, myodes glareolus schreber, 1780, microtus agrestis linnaeus, 1761, mus musculus linnaeus, 1758 and micromys minutus pallas, 1771) were collected and four shrews (sorex spp.) were by-catched. captured animals belonging to n ...201627003406
[spatio-temporal distribution of scrub typhus and related influencing factors in coastal beach area of yancheng, china].in order to provide better programs on monitoring, early warning and prevention of scrub typhus in the coastal beach area, temporal-spatial distribution characteristics of scrub typhus were summarized. relationships between temporal-spatial clustering of scrub typhus, meteorological factors, rodent distribution and the biological characteristics in coastal beach area of yancheng city, were studied.201626917522
host-parasite association in trombiculid mites (actinotrichida: trombiculidae) of temperate zone - the case of hirsutiella zachvatkini (schluger, 1948); are we dealing with prolonged contact with the host?the time-extended contact of trombiculid larvae with hosts poses a question of its ecological determinants. the phenomenon, which may facilitate the overwintering of larvae in the temperate zone, was previously observed in few parasitengone taxa, but not confirmed for mammal-associated trombiculids. the study aims at tracing the phenology of larvae of hirsutiella zachvatkini and at verifying the hypothesis of contact with the host, extending beyond the parasitic phase.201626830356
an assessment of non-volant terrestrial vertebrates response to wind farms--a study of small mammals.the majority of studies on the effects of wind energy development on wildlife have been focused on birds and bats, whereas knowledge of the response of terrestrial, non-flying vertebrates is very scarce. in this paper, the impact of three functioning wind farms on terrestrial small mammal communities (rodents and shrews) and the population parameters of the most abundant species were studied. the study was carried out in southeastern poland within the foothills of the outer western carpathians. ...201626818016
tick-borne encephalitis virus diversity in ixodid ticks and small mammals in south-western siberia, russia.the persistence of tick-borne encephalitis virus (tbev) in nature is maintained by numerous species of reservoir hosts, multiple transmissions between vertebrates and invertebrates, and the virus adaptation to its hosts. our aim: was to compare tbev isolates from ticks and small wild mammals to estimate their roles in the circulation of the viral subtypes.201627303915
seroepidemiological survey of zoonotic diseases in small mammals with pcr detection of orientia tsutsugamushi in chiggers, gwangju, korea.serosurveillance for zoonotic diseases in small mammals and detection of chiggers, the vector of orientia tsutsugamushi, were conducted from september 2014 to august 2015 in gwangju metropolitan area. apodemus agrarius was the most commonly collected small mammals (158; 91.8%), followed by myodes regulus (8; 4.6%), and crocidura lasiura (6; 3.5%). the highest seroprevalence of small mammals for o. tsutsugamushi (41; 26.3%) was followed by hantaviruses (24; 15.4%), rickettsia spp. (22; 14.1%), an ...201627417085
determination of scrub typhus suggests a new epidemic focus in the anhui province of china.in 2007, 19 cases of a scrub typhus epidemic occurred within a week at a sports school in mingguang county, anhui province, where no previous incidence of this mite borne disease had been reported. sero-surveillance in 2009 indicated that 10 of the 100 school students possessed anti-orientia tsutsugamushi antibodies. from 2009 to 2013, 60 small animals and 2250 mites were collected in the vicinity of the school. 5 of the apodemus agrarius samples and 1 group of leptotrombidium linhuaikongense te ...201626860982
prevalence of hemorrhagic fever with renal syndrome in qingdao city, china, 2010-2014.hemorrhagic fever with renal syndrome (hfrs) was considered to be transmitted by apodemus agrarius and rattus norvegicus, the principal animal hosts of hantaan virus and seoul virus, respectively. the aim of this study is to determine the correlation of hfrs incidence with capture rate and hantavirus infection rate of rodent species in qingdao city, china. we collected hfrs patients' information and captured field and residential rodents in qingdao city, china from 2010 to 2014. the correlations ...201627786303
genetic diversity and reassortment of hantaan virus tripartite rna genomes in nature, the republic of korea.hantaan virus (htnv), a negative sense tripartite rna virus of the family bunyaviridae, is the most prevalent hantavirus in the republic of korea (rok). it is the causative agent of hemorrhagic fever with renal syndrome (hfrs) in humans and maintained in the striped field mouse, apodemus agrarius, the primary zoonotic host. clinical hfrs cases have been reported commonly in hfrs-endemic areas of gyeonggi province. recently, the death of a member of the rok military from gangwon province due to h ...201627315053
hemorrhagic fever with renal syndrome, zibo city, china, 2006-2014.analysis of hemorrhagic fever with renal syndrome cases in zibo city, china, during 2006-2014 showed that it occurred year-round. peaks in spring and fall/winter were caused by hantaan and seoul viruses, respectively. rodent hosts were the striped field mouse for hantaan virus and the brown rat and house mouse for seoul virus.201626812444
[the cytogenetic consequences of chronic irradiation in rodent populations inhabiting the eastern urals radioactive trace zone].in bone marrow cells of rodents (apodemus (sylvaemus) uralensis pall., 1811, apodemus agrarius pall., 1771) inhabiting the eastern urals radioactive trace (eurt) zone (kyshtym radiation accident 1957) and adjacent areas of urals, the chromosome instability and 90sr accumulation in bones were investigated. intensive mutagenic process in both species from impact plots (the soil pollution by 90sr 2322-16690 kbq/m2) was found. significant positive correlation of aberrant cells frequencies and 90sr w ...201620968057
rickettsia spp. in small mammals and their parasitizing ectoparasites from saxony, germany.rickettsiae are emerging pathogens causing various types of spotted fever and typhus and are mostly transmitted by arthropods to humans and animals. in order to investigate the distribution of rickettsiae of the spotted fever group (sfg) in small mammals as potential reservoirs and in fleas and ticks from these animals as potential vectors, a total of 91 small mammals (seven species) were captured and their ectoparasites were collected at seven sites around leipzig, saxony, germany, in 2010 and ...201631014532
[laelaps echidninus found on skin of apodemus agrarius in wuhu area].to report the seizure of laelaps echidninus on the surface of apodemus agrarius in wuhu area for the first time, which prompts that there may exist the spread of the diseases by mites.201629469527
[cytokines and antibodies in experimental infection of wild and laboratory rodents (rodentia) with tick-borne encephalitis virus].persistence modeling was performed by means of infection of the wild rodents: northern red-backed vole myodes rutilus (pallas, 1779) and striped field mouse apodemus agrarius (pallas, 1771), as well as of laboratory mice with the tick-borne encephalitis virus (tbev) in tick suspensions with subsequent detection of the tbev, hemagglutination inhibition and virus-neutralizing antibodies, as well as expression of cytokine genes during 4 months. detection rate of the tbev rna and antigen e remained ...201729733169
chigger mite (acari: trombiculidae) survey of rodents in shandong province, northern china.chigger mites are parasites of rodents and other vertebrates, invertebrates, and other arthropods, and are the only vectors of scrub typhus, in addition to other zoonoses. therefore, investigating their distribution, diversity, and seasonal abundance is important for public health. rodent surveillance was conducted at 6 districts in shandong province, northern china (114-112°e, 34-38°n), from january to december 2011. overall, 225/286 (78.7%) rodents captured were infested with chigger mites. a ...201729103271
orientia tsutsugamushi infection in rodents in anhui province of china.we conducted an investigation of orientia tsutsugamushi infection among rodents in non-endemic areas in anhui province. fifty-six (56) rodents including 44 apodemus agrarius and 12 niviventer niviventer were trapped and captured in autumn in the fields of three counties in anhui province. dna samples were amplified and sequenced for the 56kda protein gene of orientia tsutsugamushi. the overall infection rate in the rodents was 23.2%(13/56). the rate of detection of o. tsutsugamushi in apodemus a ...201729037778
comparison of helminth community of apodemus agrarius and apodemus flavicollis between urban and suburban populations of mice.the growing human population and the development of urban areas have led to fragmentation and destruction of many natural habitats but have also created new urban habitats. these environmental changes have had a negative impact on many species of plants and animals, including parasite communities. the aim of present study was to compare the helminth communities of apodemus flavicollis and apodemus agrarius in natural and urban habitats. helminth burdens were assessed in 124 mice, 48 a. flavicoll ...201728913695
spotted fever rickettsiae in wild-living rodents from south-western poland.rickettsiae are obligate intracellular alpha-proteobacteria. they are transmitted via arthropod vectors, which transmit the bacteria between animals and occasionally to humans. so far, much research has been conducted to indicate reservoir hosts for these microorganisms, but our knowledge is still non-exhaustive. therefore, this survey was undertaken to investigate the presence of rickettsia spp. in wild-living small rodents from south-western poland.201728874204
mice as stowaways? colonization history of danish striped field mice.species from the steppe region of eastern europe likely colonized northwestern europe in connection with agriculture after 6500 bp. the striped field mouse (apodemus agrarius pallas, 1783), is a steppe-derived species often found in human crops. it is common on the southern danish islands of lolland and falster, which have been isolated from mainland europe since approximately 10 300-8000 bp. thus, this species could have been brought in with humans in connection with agriculture, or it could be ...201728679695
molecular detection by analysis of the 16s rrna gene of fecal coliform bacteria from the two korean apodemus species (apodemus agrarius and a. peninsulae).wild mouse feces can disseminate zoonotic microorganisms throughout a farm, which is a great threat to human health and can lead to economic loss through contaminated agricultural produce. to assess the microbial communities, especially fecal coliform bacteria, we used two methods. first, we isolated bacterial colonies onto the common media lb (lactose broth) agar, tsa (tryptic soy agar), and mrs (de man, rogosa, and sharpe) agar, and then randomly select colonies from each plate and stocked the ...201728525654
rodents as a reservoir of infection caused by multiple zoonotic species/genotypes of c. parvum, c. hominis, c. suis, c. scrofarum, and the first evidence of c. muskrat genotypes i and ii of rodents in europe.cryptosporidium spp. is an important causative agent of intestinal parasitoses-induced diarrhoea in humans and animals worldwide. rodents (small mammals), the main reservoir of infections, are globally expanded and overpopulated, which increases the risk of transfer of human and zoonotic pathogens from the genus cryptosporidium. in this study, cryptosporidium was detected in wild immunocompetent asymptomatic small mammals. altogether 262 fecal samples were collected from five areas in eastern sl ...201728433573
interannual cycles of hantaan virus outbreaks at the human-animal interface in central china are controlled by temperature and rainfall.hantavirus, a rodent-borne zoonotic pathogen, has a global distribution with 200,000 human infections diagnosed annually. in recent decades, repeated outbreaks of hantavirus infections have been reported in eurasia and america. these outbreaks have led to public concern and an interest in understanding the underlying biological mechanisms. here, we propose a climate-animal-hantaan virus (htnv) infection model to address this issue, using a unique dataset spanning a 54-y period (1960-2013). this ...201728696305
hantavirus rna prevalence in myomorph rodents on bolshoy ussuriysky island at the sino-russian border.to understand the distribution and infection status of hantavirus in myomorph rodents on bolshoy ussuriysky island (heixiazi island) at the sino-russian border, and to provide data for the safe development and utilization of bolshoy ussuriysky island.201728678679
hantavirus surveillance and genetic diversity targeting small mammals at camp humphreys, a us military installation and new expansion site, republic of korea.small mammal surveillance was conducted (2008-2010, 2012) at camp (cp) humphreys, a us army installation and new expansion site, republic of korea (rok), to identify hemorrhagic fever with renal syndrome health threats to us military/civilian populations during its ongoing expansion phase. small mammals were collected using sherman live capture traps and transported to korea university where they were euthanized, tissues removed, and assayed to determine hantavirus igg antibody-positive and hant ...201728448595
new definitive hosts and differential body indices of isthmiophora hortensis (digenea: echinostomatidae).the present study was performed to record new definitive hosts of isthmiophora hortensis, and to describe morphological characteristics derived from a variety of worm samples for clarification of its taxonomic validity. morphological characteristics with dimensions were observed in worm samples (n=21) from naturally infected wild animals, including a raccoon dog nyctereutes procyonoides from gimhae-si (city), gyeongsangnam-do, stray cats and a striped field mouse from several localities, and a w ...201728719953
lawsonia intracellularis in the feces of wild rodents and stray cats captured around equine farms.proliferative enteropathy is a global enteric disease of particular importance in pigs. the causative bacterium, lawsonia intracellularis, has a wide range of susceptible host species. recently, l. intracellularis has been recognized as an etiologic agent of an emerging enteric disease in foals called equine proliferative enteropathy (epe). the presence of l. intracellularis in nonruminant wildlife has raised questions regarding the role of these species in epe transmission.201728800756
[epidemiological investigation of two leptospirosis death cases in guizhou province].objective: to conduct an epidemiological investigation of two leptospirosis death cases reported in guizhou province in 2014. methods: the information of the patients were investigated and analyzed. the serological detection, samples of the two patients was detected using elisa and microscopic agglutination test (mat). leptospira carrier status of murine host animal in the living environment of the two patients was investigated in october and november of 2014. leptospires in the kidney were cult ...201728260339
sequence-independent, single-primer amplification next-generation sequencing of hantaan virus cell culture-based isolates.hantaan virus (htnv), identified in the striped field mouse (apodemus agrarius), belongs to the genus hantavirus of the family bunyaviridae and contains tripartite rna genomes, small (s), medium (m), and large (l) segments. htnv is a major causative for hemorrhagic fever with renal syndrome (hfrs) with fatality rates ranging from 1% to 15% in the republic of korea (rok) and china. defining of htnv whole-genome sequences and isolation of the infectious particle play a critical role in the charact ...201727895275
the adult and immature stages of the new species of the genus demodex (acariformes: demodecidae) with data on parasitism, topography, and topical specificity of demodecid mites in the yellow-necked mouse apodemus flavicollis (rodentia: muridae).demodex mollis n. sp. is described from the yellow-necked mouse apodemus flavicollis (melchior, 1834) from poland. it is a medium-sized demodecid mite (adult stages are on average 249 µm long) observed in the skin of the eyelid area. the new species is most similar to two other species occupying similar microhabitat in hosts of the same genus - demodex lacrimalis lukoschus and jongman, 1974 from apodemus sylvaticus linnaeus, 1758 and demodex huttereri mertens, lukoschus and nutting, 1983 from ap ...201728399378
genetic identification of prey species from teeth in faeces from the endangered leopard cat prionailurus bengalensis using mitochondrial cytochrome b gene sequence.to understand the dietary ecology of the leopard cat (prionailurus bengalensis), dna analysis was performed to identify prey species using dna isolated from teeth harvested from the faeces of this feline species. from 70 dna samples, a total of 52 mitochondrial dna (mtdna) cytochrome b (cytb) gene sequences of mammals were identified. the results of a sequence identity test indicated that those sequences were derived from four rodent species (apodemus agrarius, apodemus peninsulae, eothenomys re ...201728093009
the enzootic life-cycle of borrelia burgdorferi (sensu lato) and tick-borne rickettsiae: an epidemiological study on wild-living small mammals and their ticks from saxony, germany.borrelia burgdorferi (sensu lato) and rickettsiae of the spotted fever group are zoonotic tick-borne pathogens. while small mammals are confirmed reservoirs for certain borrelia spp., little is known about the reservoirs for tick-borne rickettsiae. between 2012 and 2014, ticks were collected from the vegetation and small mammals which were trapped in saxony, germany. dna extracted from ticks and the small mammals' skin was analyzed for the presence of rickettsia spp. and b. burgdorferi (s.l.) by ...201728285593
landscape and rodent community composition are associated with risk of hemorrhagic fever with renal syndrome in two cities in china, 2006-2013.hemorrhagic fever with renal syndrome (hfrs) is a rodent-borne disease caused by hantaviruses. landscape can influence the risk of hantavirus infection for humans, mainly through its effect on rodent community composition and distribution. it is important to understand how landscapes influence population dynamics for different rodent species and the subsequent effect on hfrs risk.201829329512
molecular epidemiology of an orientia tsutsugamushi gene encoding a 56-kda type-specific antigen in chiggers, small mammals, and patients from the southwest region of korea.a phylogenetic analysis of orientia tsutsugamushi was performed to elucidate its antigenic diversity in chiggers, small mammals, and patients. between september 2014 and december 2016, a total of 3,816 chiggers were identified within nine species of four genera in the southwest region of korea: leptotrombidium scutellare (49.9%; 1,907/3,816), leptotrombidium orientale (21.1%; 804/3,816), leptotrombidium pallidum (12.4%; 474/3,816), euchoengastia koreaensis (7.2%; 273/3,816), leptotrombidium palp ...201829313468
parallel survey of two widespread renal syndrome-causing zoonoses: leptospira spp. and hantavirus in urban environment, hungary.rodents are important reservoir hosts for several zoonotic pathogens that cause significant morbidity and mortality in humans. among others, leptospirosis is one of the most widespread zoonotic diseases worldwide and has the similar clinical manifestation with hantavirus infection in humans. despite the fact that both pathogens have great epidemiological significance in europe, no epizootiological data exist for urbanized areas so far. therefore, this study was conducted to investigate the occur ...201829437551
identification and characterization of a novel rodent bocavirus from different rodent species in china.members in the genus bocaparvovirus are closely related to human health and have a wide host range. the diverse hosts raise the possibility of crossing species barrier, which is a feature of emerging viruses. among the mammalian hosts, rodents are generally acknowledged to be important reservoirs of emerging viruses. here, rodent samples collected from six provinces and autonomous regions of china (liaoning, inner mongolia, tibet, xinjiang, guangxi and yunnan) were used to investigate the preval ...201829593218
development of 17 polymorphic microsatellite loci from jeju striped field mouse, apodemus agrarius chejuensis (rodentia: muridae), by 454 pyrosequencing.the striped field mouse, apodemus agrarius, is the most common mammal in korea. although microsatellite loci for the species have been identified from populations in southwestern china, amplification of those markers for korean populations have been unsuccessful. the complicated taxonomy of korean striped field mouse including populations on jeju island (a. a chejuensis) necessitates identification of additional molecular markers.201830275809
first detection and identification of candidatus neoehrlichia mikurensis in south korea.candidatus neoehrlichia mikurensis (ca. n. mikurensis; family anaplasmataceae) is an emerging tick-borne pathogen that causes a systemic inflammatory syndrome with thrombotic complications. we report here the first identification of ca. n. mikurensis in organ samples from small mammals captured in southwest south korea. nested pcr of groel and 16s rrna genes was used to confirm the identity of the bacteria present, and successfully amplified fragments were sequenced. all captured animals were id ...201830592745
the first finding of moniliformis moniliformis (acanthocephala, moniliformidae) in belarus.information on the detection of acanthocephalan moniliformis moniliformis (bremser, 1811) in a striped field mouse in belarus and the short description of this parasite is given. the first finding of this helminth in belarus (malorita district of brest region, south-western part of belarus) is reported. the functioning of the centre of moniliformosis on the drainage system in the malorita district is described. rodents, as well as other animals as definitive hosts and insects as intermediate hos ...201829844640
molecular evidence and diversity of the spotted-fever group rickettsia spp. in small mammals from natural, suburban and urban areas of eastern slovakia.rickettsiae of the spotted fever group are considered as emerging pathogens; ticks, fleas and mites are known to be their vectors. however, the prevalence and species diversity of rickettsiae in small mammals and the role of these hosts in the circulation of bacteria are much less studied. during 2014-2016, a total of 250 small mammals (apodemus agrarius, apodemus flavicollis, apodemus uralensis, myodes glareolus, crocidura leucodon, crocidura suaveolens, micromys minutus, microtus arvalis, micr ...201830207272
mysteries of host switching: diversification and host specificity in rodent-coccidia associations.recent studies show that host switching is much more frequent than originally believed and constitutes an important driver in evolution of host-parasite associations. however, its frequency and ecological mechanisms at the population level have been rarely investigated. we address this issue by analyzing phylogeny and population genetics of an extensive sample, from a broad geographic area, for commonly occurring parasites of the genus eimeria within the abundant rodent genera apodemus, microtus ...201829753710
[assessment of schistosomiasis transmission risk after flood damage in wuhan city].to assess the schistosomiasis transmission risk after flood damage in wuhan city.201830350504
establishment, characterization, and toxicological application of a spontaneous immortalized cell line from the striped field mouse, apodemus agrarius.it is important to secure various biological resources in situations of diminishing wildlife genetic diversity. cultured cells are useful bioresources because they can stably store genetic information for a long time and can be expanded efficiently. here, we established fibroblast cell lines from apodemus agrarius as a new living resource. a. agrarius is an important sub-predator species in ecosystem food chains and for the study of infection epidemiology. established cell lines were characteriz ...201830306320
evidence for co-invasion events: different chigger species (actinotrichida, trombidioidea: trombiculidae) share a host.cases of co-invasion of various chigger species parasitizing murids and cricetids in various habitats were analysed using morphological and molecular approaches. here we provide evidence for 25 new cases of co-parasitism of chigger mites on rodent hosts (myodes glareolus, apodemus flavicollis, apodemus agrarius) accounting for 8.6% of all host-parasite associations observed in this study. the results confirm higher incidence of co-parasitism in vertebrate-associated parasitengona mites compared ...201830206798
complete genome sequencing and comparative genomic analysis of helicobacter apodemus isolated from the wild korean striped field mouse (apodemus agrarius) for potential pathogenicity. 201830131692
the topography of rods, cones and intrinsically photosensitive retinal ganglion cells in the retinas of a nocturnal (micaelamys namaquensis) and a diurnal (rhabdomys pumilio) rodent.we used immunocytochemistry to determine the presence and topographical density distributions of rods, cones, and intrinsically photosensitive retinal ganglion cells (iprgcs) in the four-striped field mouse (rhabdomys pumilio) and the namaqua rock mouse (micaelamys namaquensis). both species possessed duplex retinas that were rod dominated. in r. pumilio, the density of both cones and rods were high (cone to rod ratio: 1:1.23) and reflected the species' fundamentally diurnal, but largely crepusc ...201830092025
morphological and molecular characterisation of eimeria vison-like oocysts (apicomplexa:eimeriidae) in farmed mink (neovison vison) in denmark.a survey was conducted on 30 danish mink farms from april to october 2016 to determine the prevalence and species of eimeria in danish farmed mink. in total, 2.6% of mink faecal samples (108/4140) were positive for eimeria vison-like oocysts by microscopy, with 24.8% (78/315) of mink being positive at least once during the study period. morphological analysis of sporulated oocysts (n = 20) identified eimeria vison-like oocysts measuring 21.0 × 13.8 μm with a length/width (l/w) ratio of 1.5. phyl ...201830003331
infections of intestinal helminth at two species of field mice, apodemus agrarius and a. peninsulae, in gangwondo and chungcheongnam-do, korea.rodents are important reservoirs of diseases affecting people and livestock, and are major sources of parasite contamination of agricultural products. we surveyed the infection status of intestinal helminths in 2 species of field mice, apodemus agrarius and a. peninsulae, captured in the agricultural fields of gangwon-do and chungcheongnam-do, korea. total 83 mice (57 a. agrarius and 26 a. peninsulae) were collected in 2 surveyed areas, and the intestines of each mouse were opened with scissors, ...201829996636
lysogenic conversion of atypical enteropathogenic escherichia coli (aepec) from human, murine, and bovine origin with bacteriophage φ3538 δstx2::cat proves their enterohemorrhagic e. coli (ehec) progeny.bacteriophages play an important role in the evolution of bacterial pathogens. a phage-mediated transfer of stx-genes to atypical enteropathogenic e. coli (aepec) which are prevalent in different hosts, would convert them to enterohemorrhagic e. coli (ehec). we decided to confirm this hypothesis experimentally to provide conclusive evidence that aepec isolated from different mammalian hosts are indeed progenitors of typical ehec which gain the ability to produce shiga-toxin by lysogeny with stx- ...201829937391
first molecular detection and phylogenetic analysis of anaplasma phagocytophilum from a clinical case of canine granulocytic anaplasmosis in japan.anaplasma phagocytophilum dna was detected from a dog with canine granulocytic anaplasmosis (cga) in japan. phylogenetic analysis of the dna using 16s rrna, glta, and groel sequences revealed that the strain was nearly identical to a. phagocytophilum detected from apodemus agrarius (black-striped field mouse) in china and korea. to our knowledge, this is the first report of molecular detection and phylogenetic analysis of a. phagocytophilum from a clinical case of cga in japan.201829709988
small mammals as sentinels of antimicrobial-resistant staphylococci.a total of 39 coagulase-negative staphylococci and seven staphylococcus aureus strains were isolated from small mammal feces, i.e., the striped field mouse (apodemus agrarius) and the yellow-necked mouse (a. flavicollis) in two sampling areas, deciduous forest and karst plains. maldi-tof analysis revealed five species of coagulase-negative staphylococci: s. sciuri, s. hominis, s. warneri, s. haemolyticus, and s. xylosus. all strains were susceptible to tetracycline, linezolid, vancomycin, and te ...201829524153
the competition between immatures of ixodes ricinus and dermacentor reticulatus (ixodida: ixodidae) ticks for rodent hosts.the numbers of ixodes ricinus (l.) and dermacentor reticulatus (fabricius) larvae and nymphs attached to small mammals are influenced by interspecific competition. the present study analyses data collected over several years in two study areas: kosewo górne (mazurian district, n poland; between july 1997 and july 2009) and białowieża primeval forest (e poland; in july 2007). in total, 975 ticks were collected from striped field mice (apodemus agrarius), yellow-necked mice (a. flavicollis), bank ...201930346558
the ecological dynamics of hantavirus diseases: from environmental variability to disease prevention largely based on data from china.hantaviruses can cause hantavirus pulmonary syndrome (hps) in the americas and hemorrhagic fever with renal syndrome (hfrs) in eurasia. in recent decades, repeated outbreaks of hantavirus disease have led to public concern and have created a global public health burden. hantavirus spillover from natural hosts into human populations could be considered an ecological process, in which environmental forces, behavioral determinants of exposure, and dynamics at the human-animal interface affect human ...201930789905
resource partitioning confirmed by isotopic signatures allows small mammals to share seasonally flooded meadows.meadows in river deltas are characterized by a high diversity and abundance of small mammals. however, neither their spatial arrangement nor differences in their use of microhabitat can necessarily explain the dense co-occurrence of sympatric species. we investigated how several small mammal species share a seasonally flooded meadow of limited size, testing predictions (p1) that herbivore, granivore, insectivore, and omnivore species are separated in time (dominant in different years), (p2) that ...201931110696
concentration of mercury in the livers of small terrestrial rodents from rural areas in poland.small terrestrial mammals could be used as accumulative biomonitors of different environmental contaminants, but the knowledge of the level of hg in their bodies is scant. the aim of our research was to verify the factors influencing hg bioaccumulation and to analyze the concentration of total mercury (hg) in the livers of four species of wild terrestrial rodents from different rural areas of poland: the yellow-necked mouse (apodemus flavicollis), striped field mouse (apodemus agrarius), common ...201931739423
[genotype and evolution of hantavirus in tiantai of zhejiang province, 2011-2018].objective: by investigating the genotype and evolutionary variation of hantavirus (hv) in tiantai county, a national surveillance site for hemorrhagic fever with renal syndrome (hfrs) was set in zhejiang province, from 2011 to 2018, to reveal the molecular epidemiological characteristics of hantavirus (hv) in tiantai. methods: total rna was extracted from ultrasound treated hv antigen- positive rat lung samples in tiantai from 2011 to 2018. after cdna was prepared, nested pcr was used to amplify ...201931658532
effects of the colour of photophase light on locomotor activity in a nocturnal and a diurnal south african rodent.many physiological and behavioural responses to varying qualities of light, particularly during the night (scotophase), have been well documented in rodents. we used varying wavelengths of day-time (photophase) lighting to assess daily responses in locomotor activity in the nocturnal namaqua rock mouse (micaelamys namaquensis) and diurnal four-striped field mouse (rhabdomys pumilio). animals were exposed to three light-dark cycle regimes: a short-wavelength- (swlc, blue), a medium-wavelength- (m ...201931573427
the prevalence of pathogenic forms of leptospira in natural populations of small wild mammals in serbia.the greatest epidemiological significance of leptospirosis in europe comes from the fact that it is the most widespread zoonosis in the world. however, epizootiological data, especially information on maintenance hosts such as small wild mammals, are largely missing. to fill this gap in data in serbia, we used rt-pcr for the detection of pathogenic leptospira species and analysed 107 animals belonging to six species of small wild mammals (apodemus agrarius, apodemus flavicollis, microtus arvalis ...201931549550
serologic survey of the crimean-congo haemorrhagic fever virus infection among wild rodents in hungary.crimean-congo haemorrhagic fever virus (cchfv) is a tick-borne pathogen, which causes an increasing number of severe infections in many parts of africa, asia and in europe. the virus is primarily transmitted by ticks, however, the spectrum of natural hosts regarding cchfv includes a wide variety of domestic and wild animals. although the presence of cchfv was hypothesized in hungary, data in support of cchfv prevalence has thus far, proven insufficient. in the present study, rodents belonging to ...201931302067
light pollution affects space use and interaction of two small mammal species irrespective of personality.artificial light at night (alan) is one form of human-induced rapid environmental changes (hirec) and is strongly interfering with natural dark-light cycles. some personality types within a species might be better suited to cope with environmental change and therefore might be selected upon under ongoing urbanization.201931215409
mow the grass at the mouse's peril: diversity of small mammals in commercial fruit farms.small mammals are not only pests but also an important part of agricultural ecosystems. the common vole is a reference species for risk assessment of plant protection products in the european union, but no data about the suitability of the species in the baltic countries are present so far. using the snap-trap line method, we evaluated species composition, abundance, and diversity of small mammal communities in commercial orchards and berry plantations in lithuania, testing the predictions that ...201931181807
analysis of the integrin β3 receptor for pathogenic orthohantaviruses in rodent host species.host reservoir specificity of pathogens is complex and may depend on receptor variability. for pathogenic orthohantaviruses, integrin β3 had been previously identified as entry receptor and the presence of aspartic acid residue at position 39 (d39) in human integrin β3 was described to be a prerequisite for infection of primate cells with hantaan virus (htnv). however, the role of integrin β3 in orthohantavirus infection of host animals is not completely understood. therefore, we analyzed the nu ...201931054291
diversity of cryptosporidium spp. in apodemus spp. in europe.the genetic diversity of cryptosporidium spp. in apodemus spp. (striped field mouse, yellow-necked mouse and wood mouse) from 16 european countries was examined by pcr/sequencing of isolates from 437 animals. overall, 13.7% (60/437) of animals were positive for cryptosporidium by pcr. phylogenetic analysis of small-subunit rrna, cryptosporidium oocyst wall protein and actin gene sequences showed the presence of cryptosporidium ditrichi (22/60), cryptosporidium apodemi (13/60), cryptosporidium ap ...201930826667
precise relative-quantity judgement in the striped field mouse apodemus agrarius pallas.applying the classical experimental scheme of training animals with food rewards to discriminate between quantities of visual stimuli, we demonstrated that not only can striped field mice apodemus agrarius discriminate between clearly distinctive quantities such as 5 and 10, but some of these mice also exhibit high accuracy in discriminating between quantities that differ only by one. the latter include both small (such as 2 versus 3) and relatively large (such as 5 versus 6, and 8 versus 9) qua ...201930707366
hepatitis e virus in common voles (microtus arvalis) from an urban environment, hungary: discovery of a cricetidae-specific genotype of orthohepevirus c.hepatitis e virus is a major causative agent of acute hepatitis worldwide. despite its zoonotic potential, there is limited information about the natural chain of hepevirus infection in wildlife, and the potential reservoir species. in this study, we performed a hev survey by heminested rt-pcr on rodent samples from an urban environment (in the city of pécs, hungary) and investigated the prevalence of the virus among these native rodent species (apodemus agrarius, apodemus flavicollis, apodemus ...201930499180
the characteristics of current natural foci of hemorrhagic fever with renal syndrome in shandong province, china, 2012-2015.hemorrhagic fever with renal syndrome (hfrs), an infectious disease caused by hantaviruses, is endemic in china and remains a serious public health problem. historically, shandong province has had the largest hfrs burden in china. however, we do not have a comprehensive and clear understanding of the current epidemic foci of hfrs in shandong province.201931107874
prevalence and genotype identification of toxoplasma gondii in suburban rodents collected at waste disposal sites.to assess the prevalence of toxoplasma gondii infection in native and commensal rodents as indicators of environmental pollution, we analyzed brain tissue from small mammals collected on legal and illegal waste sites in the slovenian and croatian parts of istria. a total of 136 animals and five species of the family muridae were analyzed: black rat (rattus rattus), domestic mouse (mus musculus), wood mouse (apodemus sylvaticus), striped field mouse (apodemus agrarius), and yellow-necked mouse (a ...201931041898
leptospirosis in zakarpattia oblast (2005-2015).background: leptospirosis occurs sporadically and as outbreaks throughout ukraine and is a nationally reportable disease. zakarpattia oblast, in the southwestern region of the country, is endemic for disease. this study examines changes in epidemic patterns from 2005 to 2015. materials and methods: suspected cases from health care services were identified based on clinical presentation and serological samples were collected. patient sera were tested by microscopic agglutination test (mat) agains ...201930835637
bartonella species detected in rodents from eastern china.bartonella are vector borne gram-negative facultative intracellular bacteria. bartonella species are associated with rodents and their flea parasites worldwide. the genetic variation and distribution of bartonella species in rodents are not clear in china. we investigated the presence and genetic diversity of bartonella species in rodents from eastern china. we captured rodents from 2015 to 2016 in jiaonan county, shandong province, and detected bartonella species in the spleen of rodents by pcr ...201931355717
long-term trends of tick-borne pathogens in regard to small mammal and tick populations from saxony, germany.rodents are important in the life-cycle of ticks as hosts for immature developmental stages. both rodents and ticks are of public health interest as they are reservoirs and vectors for different tick-borne pathogens (tbp). the aim of this study was to reassess the prevalence of tbp in previously studied areas of the city of leipzig (saxony, germany).201930909955
comparison of targeted next-generation sequencing for whole-genome sequencing of hantaan orthohantavirus in apodemus agrarius lung tissues.orthohantaviruses, negative-sense single-strand tripartite rna viruses, are a global public health threat. in humans, orthohantavirus infection causes hemorrhagic fever with renal syndrome or hantavirus cardiopulmonary syndrome. whole-genome sequencing of the virus helps in identification and characterization of emerging or re-emerging viruses. next-generation sequencing (ngs) is a potent method to sequence the viral genome, using molecular enrichment methods, from clinical specimens containing ...201931719616
hemorrhagic fever with renal syndrome.in korea, hemorrhagic fever with renal syndrome (hfrs) was first reported in a united nations (un) soldier stationed in the central front, also known as the "iron triangle". in 1976, professor ho wang lee discovered an antigen in the lung and kidney tissues of apodemus agrarius. in 1980, this novel virus was named hantaan virus after the hantaan river. the old world hantaviruses, which are usually found in east asia and europe, are generally transmitted to humans via the respiratory pathway duri ...201931668027
prevalence of orientia tsutsugamushi, anaplasma phagocytophilum and leptospira interrogans in striped field mice in gwangju, republic of korea.this study investigated the prevalence of orientia tsutsugamushi, anaplasma phagocytophilum, and leptospira interrogans in wild rodents through molecular detection using organ samples and through serological assay using blood samples of mice collected from two distinct sites in gwangju metropolitan city, republic of korea (rok). a total of 47 wild rodents, identified as apodemus agrarius (a. agrarius), were captured from june to august 2016. the seroprevalence of antibodies against bacterial pat ...201931419222
genetic characteristics of pathogenic leptospira in wild small animals and livestock in jiangxi province, china, 2002-2015.leptospirosis is one of the most important neglected tropical bacterial diseases worldwide. however, there is limited information on the genetic diversity and host selectivity of pathogenic leptospira in wild small mammal populations.201931233503
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