Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| treatment of mycobacterium abscessus infection. | mycobacterium abscessus is often resistant to multiple antimicrobial drugs, and data supporting effective drugs or dosing regimens are limited. to better identify treatment approaches and associated toxicities, we collected a series of case reports from the emerging infections network. side effects were common and often led to changing or discontinuing therapy. | 2016 | 26890211 |
| tigecycline is highly efficacious against mycobacterium abscessus pulmonary disease. | mycobacterium abscessus causes chronic pulmonary infections that are extremely difficult to cure. the currently recommended combination therapy is associated with high failure rates and relapse. tigecycline has been explored in salvage regimens, with a response rate of 43% in those who received at least a month of therapy. we performed a dose-response study in a hollow-fiber system model of pulmonary m. abscessus infection in which we recapitulated tigecycline human pulmonary concentration-time ... | 2016 | 26926649 |
| principal component analysis of maldi tof ms mass spectra separates m. abscessus (sensu stricto) from m. massiliense isolates. | the discrimination of the members of the mycobacterium abscessus complex is of clinical interest because one of the subspecies, m. massiliense, exhibits higher rates of response to antibiotic treatment for lung infection than do the other members of that complex. m. abscessus complex contains three subspecies that are laborious to identify; therefore, a routine diagnostic tool would be worthwhile. | 2016 | 26926762 |
| bactericidal and intracellular activity of β-lactams against mycobacterium abscessus. | cefoxitin and imipenem are the sole recommended β-lactams for the treatment of mycobacterium abscessus pulmonary infections. here, we investigated whether one of these drugs displays superiority in terms of killing and intracellular activity. we have also evaluated whether the use of a β-lactamase inhibitor could improve their activity. | 2016 | 26929268 |
| antimycobacterial activity of a new peptide polydim-i isolated from neotropical social wasp polybia dimorpha. | mycobacterium abscessus subsp. massiliense, a rapidly growing mycobacteria (rgm) that is becoming increasingly important among human infectious diseases, is virulent and pathogenic and presents intrinsic resistance to several antimicrobial drugs that might hamper their elimination. therefore, the identification of new drugs to improve the current treatment or lower the risk of inducing resistance is urgently needed. wasp venom primarily comprises peptides that are responsible for most of the bio ... | 2016 | 26930596 |
| a novel therapeutic approach using mesenchymal stem cells to protect against mycobacterium abscessus. | recent studies have demonstrated the therapeutic potential of mesenchymal stem cells (mscs) for the treatment of acute inflammatory injury and bacterial pneumonia, but their therapeutic applications in mycobacterial infections have not been investigated. in this study, we demonstrated the use of mscs as a novel therapeutic strategy against mycobacterium abscessus (m. abscessus), which is the most drug-resistant and difficult-to-treat mycobacterial pathogen. the systemic intravenous injection of ... | 2016 | 26946350 |
| [rapid identification of mycobacterium abscessus in cystic fibrosis patients]. | 2016 | 26946526 | |
| antibiotic treatment for nontuberculous mycobacterial lung disease. | pulmonary infections are the most frequent diseases caused by nontuberculous mycobacteria (ntm). common causative organisms of pulmonary infection are slowly growing mycobacteria including mycobacterium avium complex and mycobacterium kansasii, and rapidly growing mycobacteria including mycobacterium abscessus complex. clinical concern has been raised over the increasing incidence of ntm lung disease combined with the poor treatment outcomes of these chronic infectious diseases. since treatment ... | 2016 | 26967761 |
| update on pulmonary disease due to non-tuberculous mycobacteria. | non-tuberculous mycobacteria (ntm) are emerging worldwide as significant causes of chronic pulmonary infection, posing a number of challenges for both clinicians and researchers. while a number of studies worldwide have described an increasing prevalence of ntm pulmonary disease over time, population-based data are relatively sparse and subject to ascertainment bias. furthermore, the disease is geographically heterogeneous. while some species are commonly implicated worldwide (mycobacterium aviu ... | 2016 | 26976549 |
| epidemiology of rapidly growing mycobacteria bloodstream infections. | rapidly growing mycobacteria (rgm) bloodstream infections (bsi) are an emerging problem often associated with therapeutic challenges. we review the epidemiology, treatment and outcomes over a 5-year period of a heterogeneous group presenting to our institution with rgm bsi. | 2016 | 26992253 |
| prevalence and antimicrobial susceptibility of mycobacterium abscessus in a general hospital, china. | to gain greater insight into the prevalence drug resistant profiles of m. abscessus from a general hospital in beijing, china. | 2016 | 27003165 |
| persistent mycobacterium abscessus infection secondary to interferon-γ autoantibodies. | 2016 | 27009440 | |
| erratum for brown-elliott et al., utility of sequencing the erm(41) gene in isolates of mycobacterium abscessus subsp. abscessus with low and intermediate clarithromycin mics. | 2016 | 27016591 | |
| trehalose polyphleates are produced by a glycolipid biosynthetic pathway conserved across phylogenetically distant mycobacteria. | mycobacteria synthesize a variety of structurally related glycolipids with major biological functions. common themes have emerged for the biosynthesis of these glycolipids, including several families of proteins. genes encoding these proteins are usually clustered on bacterial chromosomal islets dedicated to the synthesis of one glycolipid family. here, we investigated the function of a cluster of five genes widely distributed across non-tuberculous mycobacteria. using defined mutant analysis an ... | 2016 | 27028886 |
| genotype ntm-dr for identifying mycobacterium abscessus subspecies and determining molecular resistance. | we studied the performance of a new line probe assay for identifying the subspecies and determining the macrolide and aminoglycoside resistance levels of 50 mycobacterium abscessus isolates. agreement of genotype ntm-dr results with sequencing and phenotypic resistance results was 92% for subspecies identification and 98% for determining molecular and phenotypic resistance. | 2016 | 27030487 |
| whole-genome sequencing and comparative analysis of mycobacterium brisbanense reveals a possible soil origin and capability in fertiliser synthesis. | mycobacterium brisbanense is a member of mycobacterium fortuitum third biovariant complex, which includes rapidly growing mycobacterium spp. that normally inhabit soil, dust and water, and can sometimes cause respiratory tract infections in humans. we present the first whole-genome analysis of m. brisbanense um_wwy which was isolated from a 70-year-old malaysian patient. molecular phylogenetic analyses confirmed the identification of this strain as m. brisbanense and showed that it has an unusua ... | 2016 | 27031249 |
| in vitro comparison of ertapenem, meropenem, and imipenem against isolates of rapidly growing mycobacteria and nocardia by use of broth microdilution and etest. | we compared the activities of the carbapenems ertapenem, meropenem, and imipenem against 180 isolates of rapidly growing mycobacteria (rgm) and 170 isolates of nocardia using the clinical and laboratory standards institute (clsi) guidelines. a subset of isolates was tested using the etest. the rate of susceptibility to ertapenem and meropenem was limited and less than that to imipenem for the rgm. analysis of major and minor discrepancies revealed that >90% of the isolates of nocardia had higher ... | 2016 | 27053677 |
| notes from the field: mycobacterium abscessus infections among patients of a pediatric dentistry practice--georgia, 2015. | on september 13, 2015, the georgia department of public health (dph) was notified by hospital a of a cluster of pediatric mycobacterium abscessus odontogenic infections. hospital a had provided care for nine children who developed presumptive or confirmed m. abscessus infection after having a pulpotomy at pediatric dentistry practice a (dates of onset: july 23, 2014-september 4, 2015). during a pulpotomy procedure, decay and the diseased pulp are removed to preserve a deciduous tooth. dph initia ... | 2016 | 27054966 |
| acquisition of mycobacterium abscessus among ventilator-dependent patients in taiwan chronic respiratory care facilities. | to investigate the acquisition of mycobacterium abscessus among ventilator-dependent patients. | 2016 | 27064349 |
| moxifloxacin's limited efficacy in the hollow-fiber model of mycobacterium abscessus disease. | current regimens used to treat pulmonary mycobacterium abscessus disease have limited efficacy. there is an urgent need for new drugs and optimized combinations and doses. we performed hollow-fiber-system studies in which m. abscessus was exposed to moxifloxacin lung concentration-time profiles similar to human doses of between 0 and 800 mg/day. the minimum bactericidal concentration and mic were 8 and 2 mg/liter, respectively, in our m. abscessus strain, suggesting bactericidal activity. measur ... | 2016 | 27067317 |
| mycobacterium abscessus as a cause of chronic meningitis: a rare clinical entity. | 2016 | 27079354 | |
| pulmonary mycobacterium abscessus infection in a patient with triple a syndrome. | gastroesophageal disorders such as achalasia can be associated with pulmonary disorders because of non-tuberculous mycobacteria, frequently masquerading as aspiration pneumonia. the optimal therapeutic regimen and duration of treatment for non-tuberculous mycobacteria lung disease is not well established. here, we present an 11 year old male patient with mycobacterium abscessus pulmonary disease and underlying triple a syndrome, who was successfully treated with 2 months of imipenem, amikacin, c ... | 2016 | 27080471 |
| rapid-growing mycobacteria infections in medical tourists: our experience and literature review. | "medical tourism" has gained popularity over the past few decades. this is particularly common with patients seeking elective cosmetic surgery in the developing world. however, the risk of severe and unusual infectious complications appears to be higher than for patients undergoing similar procedures in the united states. | 2016 | 27095310 |
| a new piperidinol derivative targeting mycolic acid transport in mycobacterium abscessus. | the natural resistance of mycobacterium abscessus to most commonly available antibiotics seriously limits chemotherapeutic treatment options, which is particularly challenging for cystic fibrosis patients infected with this rapid-growing mycobacterium. new drugs with novel molecular targets are urgently needed against this emerging pathogen. however, the discovery of such new chemotypes has not been appropriately performed. here, we demonstrate the utility of a phenotypic screen for bactericidal ... | 2016 | 27121350 |
| diagnosis and treatment of nontuberculous mycobacterial lung disease. | nontuberculous mycobacteria (ntm) are ubiquitous organisms; their isolation from clinical specimens does not always indicate clinical disease. the incidence of ntm lung diseases has been increasing worldwide. although the geographic diversity of ntm species is well known, mycobacterium avium complex (mac), m. abscessus complex (mabc), and m. kansasii are the most commonly encountered and important etiologic organisms. two distinct types of ntm lung diseases have been reported, namely fibrocavita ... | 2016 | 27134484 |
| standardized interpretation of antibiotic susceptibility testing and resistance genotyping for mycobacterium abscessus with regard to subspecies and erm41 sequevar. | the objective of this study was to provide standardized antibiotic susceptibility testing (ast) for mycobacterium abscessus with regard to subspecies. | 2016 | 27147307 |
| oral macrolide therapy following short-term combination antibiotic treatment of mycobacterium massiliense lung disease. | although mycobacterium massiliense lung disease is increasing in patients with cystic fibrosis and non-cystic fibrosis bronchiectasis, optimal treatment regimens remain largely unknown. this study aimed to evaluate the efficacy of oral macrolide therapy after an initial 2-week course of combination antibiotics for the treatment of m massiliense lung disease. | 2016 | 27167209 |
| resazurin microtiter assay for clarithromycin susceptibility testing of clinical isolates of mycobacterium abscessus group. | mycobacterium abscessus group has heterogeneous susceptibility pattern among species. the species is most common cause of nosocomial infections. macrolides minimum inhibitory concentration (mic) determination is essential for the treatment. | 2016 | 27169515 |
| successful antibiotic treatment of pulmonary disease caused by mycobacterium abscessus subsp. abscessus with c-to-t mutation at position 19 in erm(41) gene: case report. | mycobacterium abscessus complex (mabc) is the most drug resistant of the mycobacterial pathogens. m. abscessus subsp. abscessus encodes a functional erythromycin ribosomal methylase gene, erm(41), causing inducible macrolide resistance. however, some clinical isolates of m. abscessus subsp. abscessus harboring nonfunctional erm(41) were susceptible to macrolide, even after extended incubation of 14 days. loss of function of the erm(41) genes was associated with a t-to-c substitution at position ... | 2016 | 27188784 |
| isolation of nontuberculous mycobacteria from the environment of ghanian communities where buruli ulcer is endemic. | this study aimed to isolate nontuberculous mycobacterial species from environmental samples obtained from some selected communities in ghana. to optimize decontamination, spiked environmental samples were used to evaluate four decontamination solutions and supplemented media, after which the best decontamination solution and media were used for the actual analysis. the isolates obtained were identified on the basis of specific genetic sequences, including heat shock protein 65, is2404, is2606, r ... | 2016 | 27208141 |
| mycobacterium abscessus infection after facial injection with autologous fat: a case report. | we report a case of mycobacterium abscessus infection in a 29-year-old woman after facial injection with autologous fat. nineteen months previously, she received a facial surgery of autologous fat injection with the fat harvested from her inner thigh. on examination, she had multiple painful and fluctuant abscesses associated with local pyrexia in her bilateral temporal and lower orbital regions. a b ultrasound revealed multiple fat liquefaction in her bilateral temporal and lower orbital region ... | 2017 | 27220017 |
| gastric lap-band infection due to mycobacterium abscessus presenting as new-onset ascites in a cirrhotic patient. | nontuberculous mycobacteria are ubiquitous environmental organisms that are infrequently implicated as pathogens. peritoneal infection with nontuberculous mycobacteria is rare and published reports are most commonly associated with peritoneal dialysis. this study describes a case of a 41-year-old woman with cirrhosis who had mycobacterium abscessus peritonitis and an abdominal abscess resulting from infection of a remotely placed gastric band (lap-band; apollo endosurgery, inc). | 2016 | 27222118 |
| investigation of mycobacterium abscessus outbreak among cystic fibrosis patients, hawaii 2012. | 2016 | 27238609 | |
| isolation of rapidly growing nontuberculous mycobacteria in wounds following combat-related injury. | rapidly growing nontuberculous mycobacteria (rgntm) have yet to be described in combat-related injuries. this study investigates the epidemiology, clinical findings, treatment, and outcomes of rgntm infections among combat casualties wounded in afghanistan from 2010 to 2012. | 2016 | 27244062 |
| nontuberculous mycobacteria in cystic fibrosis patients on the island of gran canaria. a population study. | to determine the prevalence of nontuberculous mycobacteria (ntm) colonization and disease in cystic fibrosis (cf) patients. | 2016 | 27262751 |
| [a case of pulmonary mycobacterium abscessus infection that developed during immunosuppressive therapy for myasthenia gravis with recurrent thymoma]. | a 58-year-old man developed cough, sputum, and low-grade fever during immunosuppressive treatment with corticosteroids and cyclosporine for myasthenia gravis with recurrent thymoma. since chest ct revealed diffuse nodular opacities in both lung fields, he was referred to our department. mycobacterium abscessus was repeatedly cultured from his sputum, and he was diagnosed with pulmonary m. abscessus infection. although both chest radiological findings and clinical symptoms were mild, he required ... | 2016 | 27263226 |
| complete genome sequence of mycobacterium abscessus subsp. bolletii. | we report the complete genome sequence of a mycobacterium abscessus subsp. bolletii isolate recovered from a sputum culture from an individual with cystic fibrosis. this sequence is the first completed whole-genome sequence of m. abscessus subsp. bolletii and adds value to studies of m. abscessus complex genomics. | 2016 | 27284156 |
| identification of lysine acetylation in mycobacterium abscessus using lc-ms/ms after immunoprecipitation. | mycobacterium abscessus (mab), which manifests in the pulmonary system, is one of the neglected causes of nontuberculous mycobacteria (ntm) infection. treatment against mab is difficult, characterized by its intrinsic antibiotic drug resistance. lysine acetylation can alter the physiochemical property of proteins in living organisms. this study aimed to determine if this protein post-translational modification (ptm) exists in a clinical isolate m. abscessus gz002. we used the antiacetyl-lysine i ... | 2016 | 27323652 |
| medical management for the treatment of nontuberculous mycobacteria infection of the parotid gland: avoiding surgery may be possible. | infection with nontuberculous mycobacteria (ntm) is uncommon in the head and neck; therefore there is no clear consensus on treating these infections. our objective was to report our experience with a unique case of ntm infection of the parotid in an immunocompetent patient, in order to determine appropriate management through our experience with this pathology. a 57-year-old man, known for numerous comorbid diseases, presented to our institution complaining of right parotid swelling and pain. a ... | 2016 | 27340407 |
| analysis of pulmonary non-tuberculous mycobacterial infections after lung transplantation. | non-tuberculous mycobacteria (ntm) are important pathogens in lung transplant recipients. this study describes the spectrum of ntm respiratory tract infections and examines the association of ntm infections with lung transplant complications. | 2016 | 27368989 |
| disseminated mycobacterium abscessus complex infection manifesting as multiple areas of lymphadenitis and skin abscess in the preclinical stage of acute lymphocytic leukemia. | a 37-year-old woman was admitted to a hospital due to a prolonged fever and a rash on her legs. she had systemic lymphadenitis and a skin abscess on her left leg. pathological findings of a left leg skin biopsy revealed abscess formation with granulomatous dermatitis, mycobacterium abscessus complex was cultured from the resected left supraclavicular lymph node, and disseminated m. abscessus complex infection was diagnosed. she was treated with combination treatment with antimicrobials and percu ... | 2016 | 27374685 |
| the impact of protein extraction protocols on the performance of currently available maldi-tof mass spectrometry for identification of mycobacterial clinical isolates cultured in liquid media. | protein extraction step is particularly important for identification of mycobacterial isolates by maldi-tof mass spectrometry (ms) because of its thick and solid cell wall. this study compared the performance of maldi-tof ms for identification of mycobacterial clinical isolates cultured in liquid media between heating-based protocol and non-heating protocol. | 2016 | 27380997 |
| deletion of a dehydratase important for intracellular growth and cording renders rough mycobacterium abscessus avirulent. | mycobacterium abscessus (mabs) is a rapidly growing mycobacterium and an emerging pathogen in humans. transitioning from a smooth (s) high-glycopeptidolipid (gpl) producer to a rough (r) low-gpl producer is associated with increased virulence in zebrafish, which involves the formation of massive serpentine cords, abscesses, and rapid larval death. generating a cord-deficient mabs mutant would allow us to address the contribution of cording in the physiopathological signs of the r variant. herein ... | 2016 | 27385830 |
| non-tuberculous mycobacteria: classification, diagnostics, and therapy. | non-tuberculous mycobacteria (ntm) are species other than those belonging to the mycobacterium tuberculosis complex and do not cause leprosy. ntm are generally free-living organisms that are ubiquitous in the environment. there have been more than 140 ntm species identified to-date. they can cause a wide range of infections, with pulmonary infections being the most frequent (65-90 %). there is growing evidence that the incidence of ntm lung diseases and associated hospitalizations are on the ris ... | 2016 | 27401079 |
| mycobacterium abscessus lung infection: a case report. | 2017 | 27403004 | |
| structure elucidation and biological activity of antibacterial compound from micromonospora auratinigra, a soil actinomycetes. | the aim of this study was to isolate and characterize the bioactive compound of micromonospora auratinigra, hk-10 and its antibacterial inhibitory mechanism. | 2016 | 27406903 |
| [unclear liver lesions in a 19-year-old woman with acute myeloid leukemia]. | a 19-year old woman with acute myeloid leukemia presented with newly observed liver lesions during ongoing consolidation therapy. due to unexplained cholestasis during induction, biliary duct drainage was performed. microbiologic and histologic examinations revealed the presence of atypical mycobacteria, namely mycobacterium abscessus. with an appropriate antiinfective regime which was continuously administered using a portable pump in the outpatient setting, further mycobacterial spread during ... | 2016 | 27411791 |
| disseminated talaromyces marneffei and mycobacterium abscessus in a patient with anti-interferon-γ autoantibodies. | anti-interferon (ifn)-γ autoantibodies are increasingly recognized as a cause of adult-onset immunodeficiency and increased risk for infections with intracellular pathogens. we report on disseminated talaromyces (penicillium) marneffei and mycobacterium abscessus infection in a 72-year-old, human immunodeficiency virus noninfected, thai man with anti-ifn-γ autoantibody. the patient was successfully treated with antimicrobial therapy and rituximab to control b cell-derived autoantibodies. | 2016 | 27419165 |
| biochemical characterization of β-lactamases from mycobacterium abscessus complex and genetic environment of the β-lactamase-encoding gene. | the objectives of this study were to determine the kinetic parameters of purified recombinant blamab and blammas by spectrophotometry, analyze the genetic environment of the blamab and blammas genes in both species by polymerase chain reaction and sequencing, furthermore, in silico models of both enzymes in complex with imipenem were obtained by modeling tools. our results showed that blamab and blammas have a similar hydrolysis behavior, displaying high catalytic efficiencies toward penams, cep ... | 2017 | 27429159 |
| 18 cases of pulmonary mycobacterium abscessus: clinical difference depending on the presence or absence of mycobacterium avium complex. | it can be difficult to treat respiratory tract infections caused by mycobacterium abscessus (m. abscessus) as there is no established treatment strategy. complications involving other nontuberculous mycobacterial infections such as mycobacterium avium complex (mac) are also commonly observed. | 2016 | 27430867 |
| multistate us outbreak of rapidly growing mycobacterial infections associated with medical tourism to the dominican republic, 2013-2014(1). | during 2013, the maryland department of health and mental hygiene in baltimore, md, usa, received report of 2 maryland residents whose surgical sites were infected with rapidly growing mycobacteria after cosmetic procedures at a clinic (clinic a) in the dominican republic. a multistate investigation was initiated; a probable case was defined as a surgical site infection unresponsive to therapy in a patient who had undergone cosmetic surgery in the dominican republic. we identified 21 case-patien ... | 2016 | 27434822 |
| mycobacterium abscessus d-alanyl-d-alanine dipeptidase induces the maturation of dendritic cells and promotes th1-biased immunity. | mycobacterium abscessus, a member of the group of non-tuberculous mycobacteria, has been identified as an emerging pulmonary pathogen in humans. however, little is known about the protective immune response of antigenpresenting cells, such as dendritic cells (dcs), which guard against m. abscessus infection. the m. abscessus gene mab1843 encodes d-alanyl-d-alanine dipeptidase, which catalyzes the hydrolysis of d-alanyl-d-alanine dipeptide. we investigated whether mab1843 is able to interact with ... | 2016 | 27439605 |
| failure of the amikacin, cefoxitin, and clarithromycin combination regimen for treating pulmonary mycobacterium abscessus infection. | in a hollow-fiber model, we mimicked the drug exposures achieved in the lungs of humans treated with standard amikacin, clarithromycin, and cefoxitin combination therapy for mycobacterium abscessus infection. at optimal dosing, a kill rate of -0.09 (95% confidence interval, -0.04 to 0.03) log10 cfu per ml/day was achieved over the first 14 days, after which there was regrowth due to acquired drug resistance. thus, the standard regimen quickly failed. a new regimen is needed. | 2016 | 27458221 |
| radiologic computed tomography features of mycobacterium abscessus in cystic fibrosis. | mycobacterium abscessus infection in cystic fibrosis (cf) patients can lead to poor outcomes. early diagnosis is important, but there are no studies outlining specific imaging features of m. abscessus in cf. | 2016 | 27460837 |
| peak plasma concentration of azithromycin and treatment responses in mycobacterium avium complex lung disease. | macrolides, such as azithromycin (azm) and clarithromycin, are the cornerstones of treatment for mycobacterium avium complex lung disease (mac-ld). current guidelines recommend daily therapy with azm for cavitary mac-ld and intermittent therapy for noncavitary mac-ld, but the effectiveness of these regimens has not been thoroughly investigated. this study evaluated associations between microbiological response and estimated peak plasma concentrations (cmax) of azm. the azm cmax was measured in p ... | 2016 | 27480854 |
| mgtc as a host-induced factor and vaccine candidate against mycobacterium abscessus infection. | mycobacterium abscessus is an emerging pathogenic mycobacterium involved in pulmonary and mucocutaneous infections, presenting a serious threat for patients with cystic fibrosis (cf). the lack of an efficient treatment regimen and the emergence of multidrug resistance in clinical isolates require the development of new therapeutic strategies against this pathogen. reverse genetics has revealed genes that are present in m. abscessus but absent from saprophytic mycobacteria and that are potentiall ... | 2016 | 27481243 |
| time-to-detection of inducible macrolide resistance in mycobacterium abscessus subspecies and its association with the erm(41) sequevar. | mutations in the erm(41) gene of m.abscessus group organisms are associated with differences in inducible macrolide resistance, with current recommendations being to hold rapidly growing isolates for up to 14 days in order to ensure that resistance which develops more slowly can be detected. this study aimed to determine the ideal incubation time for accurate identification of inducible macrolide resistance as well as to determine if there was an association between the time taken to detect indu ... | 2016 | 27490181 |
| functional characterization of the mycobacterium abscessus genome coupled with condition specific transcriptomics reveals conserved molecular strategies for host adaptation and persistence. | mycobacterium abscessus subsp. abscessus (mab) is a highly drug resistant mycobacterium and the most common respiratory pathogen among the rapidly growing non-tuberculous mycobacteria. mab is also one of the most deadly of the emerging cystic fibrosis (cf) pathogens requiring prolonged treatment with multiple antibiotics. in addition to its "mycobacterial" virulence genes, the genome of mab harbours a large accessory genome, presumably acquired via lateral gene transfer including homologs shared ... | 2016 | 27495169 |
| emended description of mycobacterium abscessus, mycobacterium abscessus subsp. abscessus and mycobacteriumabscessus subsp. bolletii and designation of mycobacteriumabscessus subsp. massiliense comb. nov. | the taxonomic position of members of the mycobacterium abscessus complex has been the subject of intensive investigation and, in some aspects confusion, in recent years as a result of varying approaches to genetic data interpretation. currently, the former species mycobacterium massiliense and mycobacterium bolletii are grouped together as mycobacterium abscessus subsp. bolletii. they differ greatly, however, as the former m. bolletii has a functional erm(41) gene that confers inducible resistan ... | 2016 | 27499141 |
| clinical efficacy and safety of multidrug therapy including thrice weekly intravenous amikacin administration for mycobacterium abscessus pulmonary disease in outpatient settings: a case series. | mycobacterium abscessus (m. abscessus) pulmonary disease is a refractory chronic infectious disease. options for treating m. abscessus pulmonary disease are limited, especially in outpatient settings. among parenteral antibiotics against m. abscessus, intravenous amikacin (amk) is expected to be an effective outpatient antimicrobial therapy. this study evaluated the clinical efficacy and safety of intravenous amk therapy in outpatients with m. abscessus pulmonary disease. | 2016 | 27506679 |
| mab_3551c encodes the primary triacylglycerol synthase involved in lipid accumulation in mycobacterium abscessus. | slow growing pathogenic mycobacteria utilize host-derived lipids and accumulate large amounts of triacylglycerol (tag) in the form of intracytoplasmic lipid inclusions (ili), serving as a source of carbon and energy during prolonged infection. mycobacterium abscessus is an emerging and rapidly growing species capable to induce severe and chronic pulmonary infections. however, whether m. abscessus, like mycobacterium tuberculosis, possesses the machinery to acquire and store host lipids, remains ... | 2016 | 27513974 |
| allele-specific duplex polymerase chain reaction to differentiate mycobacterium abscessus subspecies and to detect highly clarithromycin-resistant isolates. | on the basis of the structural differences of erm, we used a duplex polymerase chain reaction (pcr) to differentiate mycobacterium abscessus subsp. abscessus and subsp. massiliense isolates and to detect the point mutations of 23s rrna gene that confer a high level of resistance to clarithromycin. subsp. massiliense strains occupying almost half of the clinical isolates can be simply identified, and their clarithromycin susceptibility can be rapidly determined. | 2017 | 27514964 |
| cystic teratoma mimicking recurrent pleural effusion, complicated by mycobacterium abscessus infection. | teratomas of anterior mediastinum are rare. they are often slow growing, asymptomatic, and detected incidentally on chest imaging. mycobacterium abscessus (m. abscessus) is an acid-fast bacillus that is classified as a pathogenic "rapid growing" non-tuberculous mycobacteria. it is an uncommon cause of human pathology, which may cause skin and soft tissue infection after skin injury following inoculation, minor trauma, and surgery. here, we present an unusual case of benign cystic teratoma mimick ... | 2016 | 27516884 |
| pseudooutbreak of rapidly growing mycobacteria due to mycobacterium abscessus subsp bolletii in a digestive and respiratory endoscopy unit caused by the same clone as that of a countrywide outbreak. | the nontuberculous mycobacteria (ntm) are widely spread. in brazil, 2,520 cases of rapidly growing mycobacteria (rgm) infections after medical procedures were reported, with 5.4% of cases related to nonsurgical invasive procedures and with an occurrence of 1 clone (bra100) of mycobacterium abscessus subsp bolletii. | 2016 | 27524260 |
| [mycobacterium abscessus pulmonary disease: important pathogen involved in microbial substitution during the treatment of non-abscessus mycobacterial disease]. | mycobacterium abscessus pulmonary disease is common in patients with bronchiectasis. however, the underlying disease that is more likely to be present in patients with m. abscessus pulmonary disease remains poorly understood. | 2016 | 27530020 |
| caspase-independent apoptosis in infected macrophages triggered by sulforaphane via nrf2/p38 signaling pathways. | mycobacterium abscessus (mabs), a non-tuberculous mycobacterium, is an emerging and rapidly growing opportunistic pathogen that is frequently found in patients with cystic fibrosis and in immunosuppressed patients. its high tolerance to antibiotics is of great concern for public health. in this study, our results showed that human thp-1-derived macrophages infected with m. abscessus presented an increase in ros production and cell necrosis. in addition, m. abscessus infection triggered activatio ... | 2015 | 27551455 |
| mycobacterium abscessus scleritis after a baerveldt glaucoma implant. | 2016 | 27552708 | |
| antibiofilm effect of antimicrobials used in the therapy of mycobacteriosis. | rapidly growing mycobacteria (rgm) are opportunistic pathogens found in the environment. when in biofilms, mycobacteria is highly resistant to antibacterial treatments. the purpose of this study is to evaluate the antibiofilm activity of antimicrobials commonly used in therapy against mycobacteria. the antimicrobial susceptibility of mycobacterium abscessus, mycobacterium fortuitum and mycobacterium massiliense was determined in planktonic and sessile populations. the antimicrobials amikacin, ci ... | 2016 | 27554273 |
| identification of antimicrobial activity among new sulfonamide metal complexes for combating rapidly growing mycobacteria. | mycobacteriosis is a type of infection caused by rapidly growing mycobacteria (rgm), which can vary from localized illness, such as skin disease, to disseminated disease. amikacin, cefoxitin, ciprofloxacin, clarithromycin, doxycycline, imipenem and sulfamethoxazole are antimicrobial drugs chosen to treat such illnesses; however, not all patients obtain the cure. the reason why the treatment does not work for those patients is related to the fact that some clinical strains present resistance to t ... | 2016 | 27567903 |
| complete genome sequencing and comparative analyses of broad-spectrum antimicrobial-producing micromonospora sp. hk10. | micromonospora genus produces >700 bioactive compounds of medical relevance. in spite of its ability to produce high number of bioactive compounds, no genome sequence is available with comprehensive secondary metabolite gene clusters analysis for anti-microbial producing micromonospora strains. thus, here we contribute the full genome sequence of micromonospora sp. hk10 strain, which has high antibacterial activity against several important human pathogens like, mycobacterium abscessus, mycobact ... | 2016 | 27609432 |
| mycobacterium abscessus esx-3 plays an important role in host inflammatory and pathological responses during infection. | mycobacterial esx systems are often related to pathogenesis during infection. however, little is known about the function of esx systems of mycobacterium abscessus (mab). this study focuses on the mab esx-3 cluster, which contains major genes such as esxh (rv0288, low molecular weight protein antigen 7; cfp-7) and esxg (rv0287, esat-6 like protein). an esx-3 (mab 2224c-2234c)-deletional mutant of mab (δesx) was constructed and used to infect murine and human macrophages. we then investigated whe ... | 2017 | 27637463 |
| non-tuberculous mycobacteria: classification, diagnostics, and therapy. | non-tuberculous mycobacteria (ntm) are species other than those belonging to the mycobacterium tuberculosis complex and do not cause leprosy. ntm are generally free-living organisms that are ubiquitous in the environment. there have been more than 140 ntm species identified to-date. they can cause a wide range of infections, with pulmonary infections being the most frequent (65-90 %). there is growing evidence that the incidence of ntm lung diseases and associated hospitalizations are on the ris ... | 2016 | 27645543 |
| survival of mycobacterium abscessus isolated from people with cystic fibrosis in artificially generated aerosols. | 2016 | 27660513 | |
| complete genome sequences of 17 rapidly growing nontuberculous mycobacterial strains. | we report the complete genome sequences of 17 rapidly growing nontuberculous mycobacterial (ntm) strains, including 16 mycobacterium abscessus complex strains and one m. immunogenum strain. these sequences add value to studies of the genetic diversity of rapidly growing ntm strains recovered from human specimens. | 2016 | 27660787 |
| cutaneous mycobacterium abscessus infection following hair transplant. | a 28-year-old man presented with a 10-day history of scalp nodules. he had undergone hair transplantation 2 months previously. incision and drainage of one of the nodules yielded gelatinous material, which was sent for microscopy and culture, including low-temperature culture. after 2 weeks of incubation, culture of the nodule yielded acid- and alcohol-fast bacilli, which were identified as mycobacterium abscessus, a rapidly growing, nontuberculous mycobacterium, which has been reported to cause ... | 2016 | 27663154 |
| fluorescent reporter ds6a mycobacteriophages reveal unique variations in infectibility and phage production in mycobacteria. | mycobacteriophage ds6a is unique among the more than 8,000 isolated mycobacteriophages due to its ability to form plaques exclusively on mycobacteria belonging to the mycobacterium tuberculosis complex (mtbc). speculation surrounding this specificity has led to unsupported assertions in published studies and patents that nontuberculous mycobacteria (ntm) are wholly resistant to ds6a infection. in this study, we identified two independent nonessential regions in the ds6a genome and replaced them ... | 2016 | 27672191 |
| evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of mycobacterium abscessus subspecies according to whole-genome sequencing. | this study was undertaken to evaluate the utility of matrix-assisted laser desorption ionization-time of flight mass spectrometry with the vitek ms plus system for identifying mycobacterium abscessus subspecies in order to facilitate more rapid and appropriate therapy. a total of 175 clinical m. abscessus strains were identified by whole-genome sequencing analysis: 139 mycobacterium abscessus subsp. abscessus and 36 mycobacterium abscessus subsp. massiliense the research-use-only (ruo) saramis k ... | 2016 | 27682129 |
| infections caused by mycobacterium abscessus: epidemiology, diagnostic tools and treatment. | mycobacterium abscessus is an emerging mycobacteria that is responsible for lung diseases and healthcare-associated extrapulmonary infections. recent findings support its taxonomic status as a single species comprising 3 subspecies designated abscessus, bolletii and massiliense. we performed a review of english-language publications investigating all three of these subspecies. areas covered: worldwide, human infections are often attributable to environmental contamination, although the isolation ... | 2016 | 27690688 |
| the best of respiratory infections from the 2015 european respiratory society international congress. | the breadth and quality of scientific presentations on clinical and translational research into respiratory infections at the 2015 european respiratory society (ers) international congress in amsterdam, the netherlands, establishes this area as one of the leadings fields in pulmonology. the host-pathogen relationship in chronic obstructive pulmonary disease, and the impact of comorbidities and chronic treatment on clinical outcomes in patients with pneumonia were studied. various communications ... | 2016 | 27730203 |
| randomized trial of liposomal amikacin for inhalation in nontuberculous mycobacterial lung disease. | lengthy, multidrug, toxic, and low-efficacy regimens limit management of pulmonary nontuberculous mycobacterial disease. | 2017 | 27748623 |
| mycobacteria clumping increase their capacity to damage macrophages. | the rough morphotypes of non-tuberculous mycobacteria have been associated with the most severe illnesses in humans. this idea is consistent with the fact that mycobacterium tuberculosis presents a stable rough morphotype. unlike smooth morphotypes, the bacilli of rough morphotypes grow close together, leaving no spaces among them and forming large aggregates (clumps). currently, the initial interaction of macrophages with clumps remains unclear. thus, we infected j774 macrophages with bacterial ... | 2016 | 27757105 |
| a laboratory-based analysis of nontuberculous mycobacterial lung disease in japan from 2012 to 2013. | since 2010, mycobacterial examination results have been used widely to survey nontuberculous mycobacteria (ntm) lung disease. | 2017 | 27788025 |
| selection of resistance to clarithromycin in mycobacterium abscessus subspecies. | mycobacterium abscessus is an emerging pathogen against which clarithromycin is the main drug used. clinical failures are commonly observed and were first attributed to acquired mutations in rrl encoding 23s rrna but were then attributed to the intrinsic production of the erm(41) 23s rna methylase. since strains of m. abscessus were recently distributed into subspecies and erm(41) sequevars, we investigated acquired clarithromycin resistance mechanisms in mutants selected in vitro from four repr ... | 2017 | 27799212 |
| clofazimine enteropathy: a rare and underrecognized complication of mycobacterial therapy. | clofazimine-induced crystal-storing histiocytosis is a rare complication of treatment previously reported in dermatology literature as a complication of leprosy therapy. we report a case of disseminated mycobacterium abscessus requiring treatment with high-dose oral clofazimine resulting in enteropathy in a patient who presented with abdominal pain, malnutrition, and melena. | 2016 | 27800519 |
| mycobacterium abscessus-induced granuloma formation is strictly dependent on tnf signaling and neutrophil trafficking. | mycobacterium abscessus is considered the most common respiratory pathogen among the rapidly growing non-tuberculous mycobacteria. infections with m. abscessus are increasingly found in patients with chronic lung diseases, especially cystic fibrosis, and are often refractory to antibiotic therapy. m. abscessus has two morphotypes with distinct effects on host cells and biological responses. the smooth (s) variant is recognized as the initial airway colonizer while the rough (r) is known to be a ... | 2016 | 27806130 |
| non-tuberculous mycobacteria: classification, diagnostics, and therapy. | non-tuberculous mycobacteria (ntm) are species other than those belonging to the mycobacterium tuberculosis complex and do not cause leprosy. ntm are generally free-living organisms that are ubiquitous in the environment. there have been more than 140 ntm species identified to-date. they can cause a wide range of infections, with pulmonary infections being the most frequent (65-90 %). there is growing evidence that the incidence of ntm lung diseases and associated hospitalizations are on the ris ... | 2017 | 27826888 |
| emergence and spread of a human-transmissible multidrug-resistant nontuberculous mycobacterium. | lung infections with mycobacterium abscessus, a species of multidrug-resistant nontuberculous mycobacteria, are emerging as an important global threat to individuals with cystic fibrosis (cf), in whom m. abscessus accelerates inflammatory lung damage, leading to increased morbidity and mortality. previously, m. abscessus was thought to be independently acquired by susceptible individuals from the environment. however, using whole-genome analysis of a global collection of clinical isolates, we sh ... | 2016 | 27846606 |
| prevalence and species spectrum of both pulmonary and extrapulmonary nontuberculous mycobacteria isolates at a tertiary care center. | nontuberculous mycobacteria (ntm) infection associated with pulmonary and extrapulmonary disease has been increasing globally. despite an increase in incidence rate of ntm infection, its prevalence, species diversity, and circulation pattern in india is largely unknown. this study sought to investigate the overall burden and diversity of ntm among both pulmonary and extrapulmonary clinical isolates from a northern indian population. | 2016 | 27847012 |
| molecular mechanisms of clarithromycin resistance in mycobacterium abscessus complex clinical isolates from venezuela. | in this study, 26 clinical isolates of mycobacterium abscessus complex strains were characterised using available identification algorithms for the three species (m. abscessus, m. massiliense and m. bolletii) and the genotypic characteristics of clarithromycin (clr) resistance were determined. strains were identified by pcr restriction fragment length polymorphism analysis of the hsp65 and erm(41) gene sequences. susceptibility to clr was determined by the broth microdilution method. the mechani ... | 2015 | 27873710 |
| use of an experimental model to evaluate infection resistance of meshes in abdominal wall surgery. | staphylococcal species are the most common organisms causing prosthetic mesh infections, however, infections due to rapidly growing mycobacteria are increasing. this study evaluates the resistance of biomaterial for abdominal wall prostheses against the development of postoperative infection in a rat model. | 2016 | 27884340 |
| eradication of mycobacterium abscessus pulmonary infection in a child with idiopathic bronchiectasis. | 2016 | 27896296 | |
| the distinct fate of smooth and rough mycobacterium abscessus variants inside macrophages. | mycobacterium abscessus is a pathogenic, rapidly growing mycobacterium responsible for pulmonary and cutaneous infections in immunocompetent patients and in patients with mendelian disorders, such as cystic fibrosis (cf). mycobacterium abscessus is known to transition from a smooth (s) morphotype with cell surface-associated glycopeptidolipids (gpl) to a rough (r) morphotype lacking gpl. herein, we show that m. abscessus s and r variants are able to grow inside macrophages and are present in mor ... | 2016 | 27906132 |
| nontuberculous mycobacteria in children: a focus on bloodstream infections. | nontuberculous mycobacteria (ntm) are ubiquitous organisms with variable disease-causing potential. bloodstream infections caused by ntm in children are poorly described. | 2017 | 27918379 |
| multidrug-resistant mycobacterium abscessus threatens patients with cystic fibrosis. | 2017 | 27956215 | |
| mycobacterium abscessus complex bacteremia due to prostatitis after prostate biopsy. | we present the case of a 49-year-old man, who developed mycobacterium abscessus complex (m. abscessus complex) bacteremia and prostatitis after prostate biopsy. the patient was successfully treated with amikacin with imipenem-cilastatin with clarithromycin. infections caused by m. abscessus complex have been increasingly described as a complication associated with many invasive procedures. invasive procedures might have contributed to the occurrence of the m. abscessus complex. although m. absce ... | 2016 | 27998503 |
| intrinsic rifamycin resistance of mycobacterium abscessus is mediated by adp-ribosyltransferase mab_0591. | rifampicin, a potent first-line tb drug of the rifamycin group, shows only little activity against the emerging pathogen mycobacterium abscessus. reportedly, bacterial resistance to rifampicin is associated with polymorphisms in the target gene rpob or the presence of enzymes that modify and thereby inactivate rifampicin. the aim of this study was to investigate the role of the mab_0591 (arrmab)-encoded rifampicin adp-ribosyltransferase (arr_mab) in innate high-level rifampicin resistance in m. ... | 2017 | 27999011 |
| antibiotic treatment for nontuberculous mycobacteria lung infection in people with cystic fibrosis. | nontuberculous mycobacteria are mycobacteria, other than those in the mycobacterium tuberculosis complex, and are commonly found in the environment. nontuberculous mycobacteria species (most commonly mycobacterium avium complex and mycobacterium abscessus) are isolated from the respiratory tract of approximately 5% to 40% of individuals with cystic fibrosis; they can cause lung disease in people with cystic fibrosis leading to more a rapid decline in lung function and even death in certain circu ... | 2016 | 28000919 |
| mycobacterial characteristics and treatment outcomes in mycobacterium abscessus lung disease. | treatment outcomes of patients with mycobacterium abscessus subspecies abscessus lung disease are poor, and the microbial characteristics associated with treatment outcomes have not been studied systematically. the purpose of this study was to identify associations between microbial characteristics and treatment outcomes in patients with m. abscessus lung disease. | 2017 | 28011608 |
| progression and treatment outcomes of lung disease caused by mycobacterium abscessus and mycobacterium massiliense. | mycobacterium abscessus and mycobacterium massiliense are grouped as the mycobacterium abscessus complex. the aim of this study was to elucidate the differences between m. abscessus and m. massiliense lung diseases in terms of progression rate, treatment outcome, and the predictors thereof. | 2017 | 28011609 |
| nontuberculous mycobacteria in cystic fibrosis. | nontuberculous mycobacteria (ntm) are found in approximately 10 % of cystic fibrosis (cf) patients, but only a portion will develop ntm disease. the management of cf lung disease should be optimized, including antibiotic therapy targeted to the individual's usual airway bacteria, prior to considering treatment for ntm lung disease. those who meet criteria for ntm lung disease may not necessarily require treatment and could be monitored expectantly if symptoms and radiographic findings are minima ... | 2016 | 28035194 |