| bronchopleural fistula following m. abscessus infection 11 years after lobectomy for lung cancer. | bronchopleural fistula (bpf) is a potentially fatal complication of lung cancer resection surgery that occurs during the healing process of the bronchial stump. however, the vulnerability of the healed surgical wound to overlapping acquired airway destruction has not yet been determined in detail. we herein present a case of fatal bpf following mycobacterium abscessus (m. abscessus) infection, which occurred 11 years after right upper lobectomy for lung cancer. the findings of the present study ... | 2013 | 24255862 |
| in vivo evaluation of antibiotic activity against mycobacterium abscessus. | the prognosis of mycobacterium abscessus infections is poor due to the lack of effective drug treatment. the objective of this study was to set up an animal model suitable to test antibiotic activity against m. abscessus. | 2014 | 24253289 |
| [drug sensitivity analysis of mycobacterium chelonae and mycobacterium abscessus and evaluation of etest for susceptibility testing]. | to study the drug resistance profile of mycobacterium(m.) chelonae and m.abscessus and to evaluate the clinical application of etest(epsilometer test) for susceptibility testing. | 2013 | 24252731 |
| gm-csf knockout mice for preclinical testing of agents with antimicrobial activity against mycobacterium abscessus. | of the non-tuberculous mycobacteria, mycobacterium abscessus is particularly refractory to antimicrobial therapy and new agents with activity against these pathogens are urgently needed. the screening of candidate antimicrobial agents against m. abscessus requires a relevant and reproducible animal model of chronic infection. granulocyte-macrophage colony-stimulating factor knockout (gm-csf ko) mice were used to develop a new animal model of chronic pulmonary m. abscessus infection that can be u ... | 2014 | 24222613 |
| characterization of rough and smooth morphotypes of mycobacterium abscessus isolates from clinical specimens. | mycobacterium abscessus, which consists of the two subspecies m. abscessus subspecies abscessus and m. abscessus subspecies bolletii, can produce rough or smooth colony morphologies. here we analyzed 50 m. abscessus isolates cultured from the respiratory specimens of 34 patients, 28 (82%) of whom had cystic fibrosis (cf), with respect to their colony morphologies and antibiotic susceptibilities. the overall proportions of occurrences of the two morphotypes were similar, with specimens from 50% o ... | 2014 | 24197890 |
| discrimination of mycobacterium abscessus subsp. massiliense from mycobacterium abscessus subsp. abscessus in clinical isolates by multiplex pcr. | the rapidly growing mycobacterium m. abscessus sensu lato is the causative agent of emerging pulmonary and skin diseases and of infections following cosmetic surgery and postsurgical procedures. m. abscessus sensu lato can be divided into at least three subspecies: m. abscessus subsp. abscessus, m. abscessus subsp. massiliense, and m. abscessus subsp. bolletii. clinical isolates of rapidly growing mycobacteria were previously identified as m. abscessus by dna-dna hybridization. more than 30% of ... | 2014 | 24197885 |
| mycobacterium abscessus scleritis following intravitreous injection of bevacizumab. | | 2013 | 24151651 |
| drug treatment of pulmonary nontuberculous mycobacterial disease in hiv-negative patients: the evidence. | pulmonary disease (pd) caused by nontuberculous mycobacteria is an emerging infection mainly in countries where the incidence of tuberculosis is in decline. it affects an elderly population, often with underlying chronic lung diseases, but its epidemiology shows significant regional variation. guidelines and recommendations for treatment of these infections exist, but build strongly on expert opinion, as very few good quality clinical trials have been performed in this field. only for the most f ... | 2013 | 24124798 |
| in vitro activity of cefoxitin and imipenem against mycobacterium abscessus complex. | the in vitro activity of cefoxitin and imipenem was compared for 43 strains of the mycobacterium abscessus complex, mostly isolated from cystic fibrosis patients. the mics of imipenem were lower than those of cefoxitin, although the number of imipenem-resistant strains was higher according to the clsi breakpoints. strain comparisons indicated that the mics of cefoxitin were significantly higher for mycobacterium bolletii than for m. abscessus. the mics of both β-lactams were higher for the rough ... | 2014 | 24112243 |
| nontuberculous mycobacteria: the changing epidemiology and treatment challenges in cystic fibrosis. | although patients with cystic fibrosis (cf) face numerous infectious pathogens over the course of their lifespan, increasing attention has recently been paid to nontuberculous mycobacteria (ntm). as reported prevalence rates rise across many countries such as the united states, the ability to recognize disease caused by ntm and subsequently treat such disease has become increasingly important. this review summarizes new observations on the epidemiology of ntm in cf as well as key elements to con ... | 2013 | 24048085 |
| an outbreak of mycobacterium abscessus infection in a pediatric intensive care unit in kuwait. | mycobacterium abscessus has been associated with respiratory tract infections, localized skin and soft tissue infections and sepsis. however, outbreaks of m. abscessus are rare. | 2014 | 24042493 |
| a case of erosive polyarthritis in a patient diagnosed with a suspicion of atypical mycobacteria. | in this report, we introduce a case of erosive polyarthritis in a 55-year-old female diagnosed with mycobacterium abscessus pulmonary infection. her arthritis has been worsened after use of dmards. the patient demonstrated a significant response to the antimicrobial regimen that was administered. we call special attention to the possibility of mycobacterium abscessus being a cause of reactive polyarthritis, particularly if symptoms worsened after use of disease-modifying antirheumatic drugs (dma ... | 2013 | 24039641 |
| insertion site and distribution of a genomic island conferring dna phosphorothioation in the mycobacterium abscessus complex. | nearly half of us clinical isolates of the emerging pathogen mycobacterium abscessus were reported to exhibit smeared dna during pfge. this dna degradation (dnd) phenotype results from dna phosphorothioation, a sulfur modification found in other bacteria and conferred by dnd genes located on mobile elements. putative dnd genes are located on a 19.6 kbp genomic island (gi) in the m. abscessus type strain atcc 19977. we confirmed that atcc 19977(t) is dnd-positive by pfge and we developed a pcr as ... | 2013 | 24014661 |
| whole-genome sequence of mycobacterium abscessus clinical strain v06705. | infection caused by mycobacterium abscessus strains is a growing cause of concern in both community-acquired and health care-associated diseases, as these organisms naturally display multiple drug resistances. we report an annotated draft genome sequence of m. abscessus strain v06705 obtained from a patient in france. | 2013 | 24009121 |
| post-operative sinus formation due to mycobacterium abscessus: a case report. | mycobacterium abscessus is ubiquitously found rapidly growing mycobacteria. although it is an uncommon pathogen, it has been known to cause cutaneous infection following inoculation, minor trauma or surgery. this communication reports an immuno-competent patient developing multiple sinuses due to mycobacterium abscessus in the post- operative period. | 2013 | 24000496 |
| identification and characterization of the genetic changes responsible for the characteristic smooth-to-rough morphotype alterations of clinically persistent mycobacterium abscessus. | mycobacterium abscessus is an emerging pathogen that is increasingly recognized as a relevant cause of human lung infection in cystic fibrosis patients. this highly antibiotic-resistant mycobacterium is an exception within the rapidly growing mycobacteria, which are mainly saprophytic and non-pathogenic organisms. m. abscessus manifests as either a smooth (s) or a rough (r) colony morphotype, which is of clinical importance as r morphotypes are associated with more severe and persistent infectio ... | 2013 | 23998761 |
| simultaneous detection of mycobacterium tuberculosis complex and nontuberculous mycobacteria in respiratory specimens. | many nontuberculous mycobacteria (ntm) species have clinical significance, and the rapid and reliable identification of mycobacterium tuberculosis complex (mtbc) and ntm species is important. we evaluated the simultaneous detection of mtbc and ntm in respiratory specimens. mtbc and ntm were simultaneously detected and identified by laboratory-developed (ldt) real-time pcr, multiplex real-time pcr/melting curve analysis, rpob pcr restriction fragment length polymorphisms and the advansure mycobac ... | 2013 | 23988279 |
| non-tuberculous mycobacteria: a retrospective review of scottish isolates from 2000 to 2010. | there is growing recognition of the clinical importance of non-tuberculous mycobacteria (ntm), a group of versatile opportunistic bacterial pathogens. we describe the characteristics of ntm isolates in scotland over an 11-year period using data held by the scottish mycobacteria reference laboratory. american thoracic society microbiological criteria were used to evaluate the clinical significance of isolates. data presented include analysis of trends across time, species/body site associations, ... | 2014 | 23986391 |
| normalization of elevated ca 19-9 level after treatment in a patient with the nodular bronchiectatic form of mycobacterium abscessus lung disease. | carbohydrate antigen 19-9 (ca 19-9) is a widely-used tumor marker in patients with pancreatic cancer. however, some patients with respiratory disease also exhibit elevated serum ca 19-9 levels. we report a case of normalization of elevated serum ca 19-9 levels after treatment of the nodular bronchiectatic form of mycobacterium ab scessus lung disease. a 40-year-old man visited our hospital because of chronic cough and sputum. a computed tomography scan revealed severe bronchiectasis in the right ... | 2013 | 23946755 |
| in vitro activity of amikacin against isolates of mycobacterium avium complex with proposed mic breakpoints and finding of a 16s rrna gene mutation in treated isolates. | amikacin is a major drug used for the treatment of mycobacterium avium complex (mac) disease, but standard laboratory guidelines for susceptibility testing are not available. this study presents in vitro amikacin mics for 462 consecutive clinical isolates of the mac using a broth microdilution assay. approximately 50% of isolates had amikacin mics of 8 μg/ml, and 86% had mics of ≤16 μg/ml. of the eight isolates (1.7%) with mics of 64 μg/ml, five had an mic of 32 μg/ml on repeat testing. ten isol ... | 2013 | 23946523 |
| mixed mycobacterial infection in an adult koi carp cyprinus carpio l. | | 2014 | 23937602 |
| vertebral osteomyelitis caused by mycobacterium abscessus. | mycobacterium infection caused by non-tuberculous mycobacterial (ntbm) organisms is becoming more common. although ntbm osteomyelitis is unusual, it can occur in otherwise healthy individuals, but it is also associated with immunocompromised states, such as steroidal therapy and aids, and may be observed following trauma. mycobacterium avium is reported to be the most common causative agent, and mycobacterium abscessus has only been described in two cases. we report the case of a 44-year-old man ... | 2013 | 23925676 |
| standardization of multilocus sequence typing scheme for mycobacterium abscessus and mycobacterium massiliense. | this study aims to develop a multilocus sequence typing (mlst) scheme for mycobacterium abscessus complex for the typing of stains within each species. a total of 89 clinical isolates of m. abscessus complex from 71 patients of 2 tertiary care hospitals in south korea were included. forty-two isolates were identified as m. abscessus, and 29, as mycobacterium massiliense through sequencing of 8 housekeeping genes and rpob. the mlst scheme identified 26 different sequence types(sts) and 13 differe ... | 2013 | 23891467 |
| mycobacterium abscessus peritonitis associated with laparoscopic gastric banding. | mycobacterium abscessus is a rapidly growing mycobacterium that is a common water contaminant in the environment. we report a case of m. abscessus infection with band erosion following laparoscopic gastric banding. | 2013 | 23855515 |
| mab_3168c, a putative acetyltransferase, enhances adherence, intracellular survival and antimicrobial resistance of mycobacterium abscessus. | mycobacterium abscessus is a non-tuberculous mycobacterium. it can cause diseases in both immunosuppressed and immunocompetent patients and is highly resistant to multiple antimicrobial agents. m. abscessus displays two different colony morphology types: smooth and rough morphotypes. cells with a rough morphotype are more virulent. the purpose of this study was to identify genes responsible for m. abscessus morphotype switching. with transposon mutagenesis, a mutant with a tn5 inserted into the ... | 2013 | 23840740 |
| potentiation of high hydrostatic pressure inactivation of mycobacterium by combination with physical and chemical conditions. | mycobacterium abscessus is an important hospital-acquired pathogen involved in infections associated with medical, surgical, and biopharmaceutical materials. in this work, we investigated the pressure-induced inactivation of two strains [2544 and american type culture collection (atcc) 19977] of m. abscessus in combination with different temperatures and ph conditions. for strain 2544, exposure to 250 mpa for 90 min did not significantly inactivate the bacteria at 20 °c, whereas at -15 °c, there ... | 2013 | 23836348 |
| complete genome sequence of mycobacterium massiliense clinical strain asan 50594, belonging to the type ii genotype. | we report the complete genome sequence of the mycobacterium massiliense clinical strain asan 50594, which was grouped into the m. massiliense type ii genotype, isolated from a korean patient. this genome sequence will serve as a valuable reference for understanding the disparity in virulence and epidemiological traits between strains belonging to the mycobacterium abscessus complex. | 2013 | 23833135 |
| drosophila melanogaster model for mycobacterium abscessus infection. | mycobacterium abscessus is a human pathogen that is responsible for a broad spectrum of tissue infections and disseminated infections in immunodeficient patients. this pathogen is one of the most resistant organisms to chemotherapeutic agents. therefore, we tested the hypothesis that the fruit fly, drosophila melanogaster, is a genetically tractable model host for m. abscessus. in this context, we infected d. melanogaster with m. abscessus. this m. abscessus infection results in dissemination in ... | 2013 | 23831804 |
| thoracic empyema and bacteremia due to mycobacterium abscessus in a patient with liver cirrhosis. | empyema thoracis with concomitant bacteremia caused by nontuberculous mycobacterium is rare. herein, we report a case of disseminated mycobacterium abscessus in a patient with liver cirrhosis and diabetes mellitus. m. abscessus was isolated from the specimen of pleural fluid and blood. the patients clinical condition gradually improved after antibiotic use and drainage. | 2013 | 23831159 |
| nontuberculous mycobacteria in respiratory samples from patients with pulmonary tuberculosis in the state of rondônia, brazil. | the main cause of pulmonary tuberculosis (tb) is infection with mycobacterium tuberculosis (mtb). we aimed to evaluate the contribution of nontuberculous mycobacteria (ntm) to pulmonary disease in patients from the state of rondônia using respiratory samples and epidemiological data from tb cases. mycobacterium isolates were identified using a combination of conventional tests, polymerase chain reaction-based restriction enzyme analysis of hsp65 gene and hsp65 gene sequencing. among the 1,812 ca ... | 2013 | 23827995 |
| mycobacterium abscessus bacteremia after receipt of intravenous infusate of cytokine-induced killer cell therapy for body beautification and health boosting. | we report the first series of mycobacterium abscessus bacteremia after cytokine-induced killer cell therapy for body beautification and health boosting. | 2013 | 23825355 |
| lung transplantation for cystic fibrosis and bronchiectasis. | lung transplantation has become an excellent treatment option for patients with cystic fibrosis (cf) and bronchiectasis with very advanced lung disease. despite the challenges that the cf patients present, survival is more favorable than that seen in patients with chronic obstructive pulmonary disease and pulmonary fibrosis. although those cf and bronchiectasis patients with severe respiratory disease are often infected with organisms that display in vitro resistance to the commonly used antibio ... | 2013 | 23821505 |
| recent advances in tuberculosis and nontuberculous mycobacteria lung disease. | tuberculosis (tb) is one of the largest health problems in the world today. and the incidence of nontuberculous mycobacteria (ntm) lung disease appears to be increasing worldwide. recently, an automated, nucleic acid amplification assay for the rapid detection of both mycobacterium tuberculosis and rifampin resistance was developed (xpert mtb/rif). and fixed-dose combinations of anti-tb drugs and linezolid have been introduced in the treatment of tb. and new ntm species, named mycobacterium mass ... | 2013 | 23814596 |
| new rapid scheme for distinguishing the subspecies of the mycobacterium abscessus group and identifying mycobacterium massiliense isolates with inducible clarithromycin resistance. | mycobacterium abscessus (m. abscessus sensu lato, or the m. abscessus group) comprises three closely related taxa whose taxonomic statuses are under revision, i.e., m. abscessus sensu stricto, mycobacterium bolletii, and mycobacterium massiliense. we describe here a simple, robust, and cost-effective pcr-based method for distinguishing among m. abscessus, m. massiliense, and m. bolletii. based on the m. abscessus atcc 19977(t) genome, regions that discriminated between m. abscessus and m. massil ... | 2013 | 23804391 |
| adherence and biofilm formation of mycobacterium avium, mycobacterium intracellulare and mycobacterium abscessus to household plumbing materials. | measure adherence and biofilm formation by cells of mycobacterium avium, mycobacterium intracellulare and mycobacterium abscessus on common household plumbing materials namely stainless steel, glass, zinc-galvanized steel, copper and polyvinyl chloride (pvc). | 2013 | 23742161 |
| serodiagnosis of mycobacterium avium complex and mycobacterium abscessus complex pulmonary disease by use of iga antibodies to glycopeptidolipid core antigen. | an enzyme immunoassay kit that detects serum iga antibody reacting to glycopeptidolipid core antigen derived from mycobacterium avium complex (mac) was not useful for differentiating mac pulmonary disease (pd) from mycobacterium abscessus complex pd (mab-pd). however, this assay could be useful for differentiating mac- and mab-pd from pulmonary tuberculosis. (this study has been registered at clinicaltrials.gov under registration no. nct00970801.). | 2013 | 23740728 |
| emergence of clinically relevant non-tuberculous mycobacterial infections in saudi arabia. | non-tuberculous mycobacteria (ntm) are emerging around the world due to a higher prevalence of immunosuppressive illness and therapy. saudi arabia is not an exception as there have been novel mycobacterial species also identified. in addition, several published case reports from different parts of the country suggest a growing pathogenic potential of ntm. as the first nationwide study, we sought to gain an insight into the species diversity of ntm clinical isolates. | 2013 | 23738024 |
| catheter related infection due to mycobacterium abscessus in a patient under peritoneal dialysis. | | 2013 | 23735153 |
| extrapulmonary infections caused by a dominant strain of mycobacterium massiliense (mycobacterium abscessus subspecies bolletii). | a single strain of mycobacterium massiliense (bra 100), a subspecies of the mycobacterium abscessus complex, has been responsible for an epidemic of post-surgical infections in brazil. outside brazil, this is the first report to describe a single emerging strain of m. massiliense (tpe 101) associated with extrapulmonary infections. this phenomenon may be underestimated because sophisticated molecular typing of m. abscessus is not routinely performed. our molecular epidemiology study was triggere ... | 2013 | 23718188 |
| epidemiology and outcomes of deep surgical site infections following lung transplantation. | we conducted a retrospective study of deep surgical site infections (ssis) among consecutive patients who underwent lung transplantation (ltx) at a single center from 2006 through 2010. thirty-one patients (5%) developed ssis at median 25 days after ltx. empyema was most common (42%), followed by surgical wound infections (29%), mediastinitis (16%), sternal osteomyelitis (6%), and pericarditis (6%). pathogens included gram-positive bacteria (41%), gram-negative bacteria (41%), fungi (10%) and my ... | 2013 | 23710593 |
| lung transplant outcomes in cystic fibrosis patients with pre-operative mycobacterium abscessus respiratory infections. | mycobacterium abscessus in cystic fibrosis (cf) patients is considered a contraindication to lung transplantation. we examine the post-transplant outcomes of cf patients with m. abscessus pre-transplant. | 2013 | 23710571 |
| mycobacterium abscessus isolated from municipal water - a potential source of human infection. | mycobacterium abscessus is a rapidly growing mycobacterium responsible for progressive pulmonary disease, soft tissue and wound infections. the incidence of disease due to m. abscessus has been increasing in queensland. in a study of brisbane drinking water, m. abscessus was isolated from ten different locations.the aim of this study was to compare genotypically the m. abscessus isolates obtained from water to those obtained from human clinical specimens. | 2013 | 23705674 |
| cutaneous inoculation of nontuberculous mycobacteria during professional tattooing: a case series and epidemiologic study. | the increase in popularity of tattoos has coincided with an increase in reports of cutaneous inoculation of nontuberculous (atypical) mycobacteria (ntm) during the tattooing process. we report 3 ntm infections in otherwise healthy persons who received tattoos, which prompted a multiagency epidemiologic investigation. | 2013 | 23704119 |
| molecular longitudinal tracking of mycobacterium abscessus spp. during chronic infection of the human lung. | the mycobacterium abscessus complex is an emerging cause of chronic pulmonary infection in patients with underlying lung disease. the m. abscessus complex is regarded as an environmental pathogen but its molecular adaptation to the human lung during long-term infection is poorly understood. here we carried out a longitudinal molecular epidemiological analysis of 178 m. abscessus spp. isolates obtained from 10 cystic fibrosis (cf) and 2 non cf patients over a 13 year period. multi-locus sequence ... | 2013 | 23696800 |
| clinical isolates of mycobacterium abscessus in guangzhou area most possibly from the environmental infection showed variable susceptibility. | mycobacterium abscessus (m. abscessus) can cause a variety of human infections, involving the lung, skin and soft tissues, and is generally believed to be acquired from environmental sources. the aim of this study was to investigate the molecular diversity and antibiotic susceptibility of m. abscessus isolates as the basis for strategies to improve control and management of infection. | 2013 | 23673103 |
| [new light on the contagiousness of mycobacterium abscessus]. | | 2013 | 23659159 |
| mabsbase: a mycobacterium abscessus genome and annotation database. | mycobacterium abscessus is a rapidly growing non-tuberculous mycobacterial species that has been associated with a wide spectrum of human infections. as the classification and biology of this organism is still not well understood, comparative genomic analysis on members of this species may provide further insights on their taxonomy, phylogeny, pathogenicity and other information that may contribute to better management of infections. the mabsbase described in this paper is a user-friendly databa ... | 2013 | 23658631 |
| transition metal-α-amino acid complexes with antibiotic activity against mycobacterium spp. | synthetic iridium-, rhodium-, and ruthenium-amino acid complexes with hydrophobic l-amino acids have antibiotic activity against mycobacterium spp., including mycobacterium bovis bcg and the rapidly growing species mycobacterium abscessus and mycobacterium chelonae. concentrations of transition metal-amino acid complexes demonstrating hemolysis or cytotoxicity were 10- to 25-fold higher than were the mics. | 2013 | 23650159 |
| paediatric lung transplant outcomes vary with mycobacterium abscessus complex species. | | 2013 | 23633613 |
| nontuberculous mycobacterial infection after fractionated co(2) laser resurfacing. | nontuberculous mycobacteria are increasingly associated with cutaneous infections after cosmetic procedures. fractionated co2 resurfacing, a widely used technique for photorejuvenation, has been associated with a more favorable side effect profile than alternative procedures. we describe 2 cases of nontuberculous mycobacterial infection after treatment with a fractionated co2 laser at a private clinic. densely distributed erythematous papules and pustules developed within the treated area within ... | 2013 | 23628077 |
| high recurrence rate of lymphadenitis due to nontuberculous mycobacteria and its association with concurrent salmonella infection in taiwan. | the objective of this study is to investigate the clinical characteristics of lymphadenitis due to nontuberculous mycobacteria (ntm) in taiwan. | 2014 | 23615164 |
| rapid diagnosis of mycobacterium abscessus endophthalmitis. | nontuberculous mycobacteria are widely distributed in the environment and have the potential to cause a wide spectrum of infections including pulmonary, bone, soft tissue or ocular infections. they are a rare cause of endophthalmitis, a potentially devastating condition, which may be acquired through contamination of water or antiseptic solutions. diagnosis is often delayed due to low clinical suspicion, resulting in poor clinical outcomes. newer laboratory techniques such as real-time pcr can b ... | 2013 | 23579395 |
| whole-genome sequencing to identify transmission of mycobacterium abscessus between patients with cystic fibrosis: a retrospective cohort study. | increasing numbers of individuals with cystic fibrosis are becoming infected with the multidrug-resistant non-tuberculous mycobacterium (ntm) mycobacterium abscessus, which causes progressive lung damage and is extremely challenging to treat. how this organism is acquired is not currently known, but there is growing concern that person-to-person transmission could occur. we aimed to define the mechanisms of acquisition of m abscessus in individuals with cystic fibrosis. | 2013 | 23541540 |
| identification and characterization of potential therapeutic candidates in emerging human pathogen mycobacterium abscessus: a novel hierarchical in silico approach. | mycobacterium abscessus, a non-tuberculous rapidly growing mycobacterium, is recognized as an emerging human pathogen causing a variety of infections ranging from skin and soft tissue infections to severe pulmonary infections. lack of an optimal treatment regimen and emergence of multi-drug resistance in clinical isolates necessitate the development of better/new drugs against this pathogen. the present study aims at identification and qualitative characterization of promising drug targets in m. ... | 2013 | 23527108 |
| expansion of functional human mucosal-associated invariant t cells via reprogramming to pluripotency and redifferentiation. | mucosal-associated invariant t (mait) cells play an important physiological role in host pathogen defense and may also be involved in inflammatory disorders and multiple sclerosis. the rarity and inefficient expansion of these cells have hampered detailed analysis and application. here, we report an induced pluripotent stem cell (ipsc)-based reprogramming approach for the expansion of functional mait cells. we found that human mait cells can be reprogrammed into ipscs using a sendai virus harbor ... | 2013 | 23523177 |
| mycobacterial genotypes are associated with clinical manifestation and progression of lung disease caused by mycobacterium abscessus and mycobacterium massiliense. | mycobacterium abscessus and mycobacterium massiliense, which cause lung disease, are variable in their clinical manifestation and progression. we hypothesized that mycobacterial genotypes represent their pathogenic phenotypes, which would result in particular genotypes being associated with disease progression. | 2013 | 23511298 |
| mycobacterium abscessus complex lung infection in a toddler with a tracheostomy. | mycobacterium abscessus complex and other non-tuberculous mycobacteria are infrequently encountered respiratory pathogens in patients with tracheostomies. we report a 4-year-old girl with a tracheostomy, placed during infancy for management of severe bronchopulmonary dysplasia and laryngeal stenosis, who developed a m. abscessus complex lung infection. there was clear evidence of parenchymal involvement and true infection beyond colonization. she demonstrated dramatic clinical, laboratory, and r ... | 2014 | 23460506 |
| mycobacterium abscessus induces a limited pattern of neutrophil activation that promotes pathogen survival. | mycobacterium abscessus is a rapidly growing mycobacterium increasingly detected in the neutrophil-rich environment of inflamed tissues, including the cystic fibrosis airway. studies of the immune reaction to m. abscessus have focused primarily on macrophages and epithelial cells, but little is known regarding the neutrophil response despite the predominantly neutrophillic inflammation typical of these infections. in the current study, human neutrophils released less superoxide anion in response ... | 2013 | 23451220 |
| pulmonary mycobacterium abscessus disease in a patient receiving low-dose methotrexate for treatment of early rheumatoid arthritis. | a 70-year-old woman with methotrexate (mtx)-refractory rheumatoid arthritis (ra) was referred to our hospital for introduction of biological therapy. on high-resolution computed tomography scans, the patient exhibited abnormal findings such as bronchiectasis and centrilobular small nodules, which were highly suggestive of pulmonary nontuberculous mycobacterial (ntm) disease. although mycobacterial cultures of sputum specimens yielded negative results, cultures of bronchoalveolar lavage fluids gr ... | 2013 | 23430370 |
| whole-genome shotgun sequencing of mycobacterium abscessus m156, an emerging clinical pathogen in malaysia. | mycobacterium abscessus is an emerging clinical pathogen commonly associated with non-tuberculous mycobacterial infections. we report herein the draft genome of m. abscessus strain m156. | 2013 | 23405341 |
| cystic fibrosis cfbe41o- cells contain tlr1 snp i602s and fail to respond to mycobacterium abscessus. | mycobacterium abscessus causes lung infection in patients with cystic fibrosis. m. abscessus stimulates the host innate immune response via tlr2 on respiratory epithelial cells. signaling through tlr2 requires the formation of tlr2/tlr1 heterodimers on the cell surface. | 2013 | 23403223 |
| synergistic activities of tigecycline with clarithromycin or amikacin against rapidly growing mycobacteria in taiwan. | the occurrence of diseases caused by rapidly growing mycobacteria (rgm) is increasing in taiwan. in this study, the in vitro antimicrobial activities of tigecycline, minocycline, tetracycline and doxycycline were evaluated against 160 clinical rgm isolates, including 34 mycobacterium abscessus sensu stricto (s.s.), 44 mycobacterium massiliense, 1 mycobacterium bolletii, 58 mycobacterium fortuitum and 23 mycobacterium chelonae. clarithromycin and amikacin were tested alone as well as for synergis ... | 2013 | 23312605 |
| mycobacterium abscessus multispacer sequence typing. | mycobacterium abscessus group includes antibiotic-resistant, opportunistic mycobacteria that are responsible for sporadic cases and outbreaks of cutaneous, pulmonary and disseminated infections. however, because of their close genetic relationships, accurate discrimination between the various strains of these mycobacteria remains difficult. in this report, we describe the development of a multispacer sequence typing (mst) analysis for the simultaneous identification and typing of m. abscessus my ... | 2013 | 23294800 |
| catheter-related mycobacterium abscessus bacteremia manifested with skin nodules, pneumonia, and mediastinal lymphadenopathy. | although previously rare, catheter-related bloodstream infection caused by rapidly growing mycobacteria is now increasingly encountered, especially among cancer patients who have catheters implanted for chemotherapy treatments. a 73-year-old female patient with acute myeloid leukemia (aml) had mycobacterium abscessus bacteremia with manifestations of multiple skin nodules, pneumonia, and mediastinal lymphadenopathy 4 months after the implantation of a peripherally inserted central catheter (picc ... | 2013 | 23257257 |
| antibiotic treatment for nontuberculous mycobacteria lung infection in people with cystic fibrosis. | nontuberculous mycobacteria are mycobacteria, other than those in the mycobacterium tuberculosis complex, and are commonly found in the environment. nontuberculous mycobacteria species (most commonly mycobacterium avium complex and mycobacterium abscessus) are isolated from the respiratory tract of approximately 5% to 20% of individuals with cystic fibrosis; they can cause lung disease in people with cystic fibrosis leading to more a rapid decline in lung function and even death in certain circu ... | 2012 | 23235684 |
| impaired expression of mapk is associated with the downregulation of tnf-α, il-6, and il-10 in mycobacterium abscessus lung disease. | healthy individuals who develop nontuberculous mycobacteria (ntm) lung disease are likely to have specific susceptibility factors which can lead to a ntm infection. the aim of the present study was to investigate the mechanism underlying innate immune responses, including the role of mitogen-activated protein kinase (mapk), in mycobacterium abscessus lung disease. | 2012 | 23227067 |
| increased incidence of cutaneous nontuberculous mycobacterial infection, 1980 to 2009: a population-based study. | to determine the incidence and clinical characteristics of cutaneous nontuberculous mycobacterial (ntm) infection during the past 30 years and whether the predominant species have changed. | 2013 | 23218797 |
| are we overlooking infections owing to non-tuberculous mycobacteria during routine conventional laboratory investigations? | a large number of potentially pathogenic non-tuberculous mycobacteria (ntm) encountered in the clinical laboratory makes it necessary to identify their species to ensure appropriate treatment. however, labor-intensive conventional methods of speciation are not used in every laboratory, and hence ntm infections are often ignored. polymerase chain reaction (pcr) restriction analysis (pra) was applied in this study for early identification and speciation of mycobacterial species on 306 cultures of ... | 2012 | 26785625 |
| endovascular mycobacterium abscessus infection in a heart transplant recipient: a case report and review of the literature. | nontuberculous mycobacteria are ubiquitous in the environment. although rarely a cause of infection in immunocompetent individuals, increased risk and severity of infection are seen in patients who are immunocompromised, such as those with solid organ transplants. in this report, we describe the first case of disseminated endovascular mycobacterium abscessus in a heart transplant recipient. a review of the literature regarding this infection in heart transplant recipients and its therapeutic opt ... | 2013 | 23176090 |
| [comparison of clinical manifestations between mycobacterium avium-intracellulare complex and mycobacterium abscessus pulmonary diseases]. | to compare the clinical manifestations of nontuberculous mycobacterial (ntm) pulmonary diseases caused by mycobacterium avium-intracellulare complex (mac) and mycobacterium abscessus. | 2012 | 23158006 |
| pulmonary sequestration infected with nontuberculous mycobacteria: a report of two cases and literature review. | we report two cases of pulmonary sequestration infected with nontuberculous mycobacteria (ntm): mycobacterium avium and mycobacterium abscessus. chest computed tomography showed pneumonic consolidation in the right lower lobe, which received a systemic blood supply from the descending aorta in both patients. video-assisted thoracoscopic surgeries were successfully performed and pathological examinations revealed multiple caseating granulomas. a review of the literature revealed only seven previo ... | 2012 | 23146810 |
| genome sequence of mycobacterium abscessus strain m152. | mycobacterium abscessus is an environmental bacterium with increasing clinical relevance. here, we report the annotated whole-genome sequence of m. abscessus strain m152. | 2012 | 23144407 |
| comparative drug resistance of mycobacterium abscessus and m. chelonae isolates from patients with and without cystic fibrosis in the united kingdom. | the isolation of rapidly growing mycobacteria (rgm), particularly mycobacterium abscessus, from individuals with cystic fibrosis (cf) is associated with poor clinical outcome due to broad drug resistance and the difficulty of eradicating the organisms. susceptibility testing is recommended to guide therapy. a disc diffusion method is used in the united kingdom, whereas in the united states, the clsi (clinical and laboratory standards institute) recommends the broth dilution method. the purpose o ... | 2013 | 23135941 |
| treatment of mycobacterium abscessus: all macrolides are equal, but perhaps some are more equal than others. | | 2012 | 23118083 |
| mycobacterium abscessus lung disease in a patient with previous pulmonary tuberculosis. | patients with pre-existing lung damage, such as due to pulmonary tuberculosis (ptb), are susceptible to nontuberculous mycobacteria (ntm) infections. for patients with previous ptb, it is difficult to differentiate ntm lung disease from ptb, especially in tuberculosis (tb) high-burdened countries. here, we report a case of mycobacterium abscessus lung disease with a previous history of ptb. the patient underwent a right upper lobe lobectomy due to disappointing treatment response with anti-tuber ... | 2012 | 23077819 |
| genomic analysis of mycobacterium abscessus strain m139, which has an ambiguous subspecies taxonomic position. | mycobacterium abscessus is a ubiquitous, rapidly growing species of nontuberculous mycobacteria that colonizes organic surfaces and is frequently associated with opportunistic infections in humans. we report here the draft genome sequence of mycobacterium abscessus strain m139, which shows genomic features reported to be characteristic of both mycobacterium abscessus subsp. abscessus and mycobacterium abscessus subsp. massiliense. | 2012 | 23045507 |
| impact of molecular diagnosis on treating mendelian susceptibility to mycobacterial diseases. | the il-12-ifn-γ axis is critical for immune defense against mycobacterial infections. inherited mutations that affect normal activation of this self-amplifying cytokine reaction lead to increased chances of mycobacterial infections, known as mendelian susceptibility to mycobacterial diseases (msmd). delayed diagnosis and difficulty in identifying pathogenic mycobacteria hinder proper treatment of patients, so the aim of this study was to facilitate the diagnosis of mycobacterial infections in ms ... | 2012 | 23036270 |
| phagocytosis and cytokine response to rough and smooth colony variants of mycobacterium abscessus by human peripheral blood mononuclear cells. | mycobacterium abscessus is a non-tuberculous mycobacteria able to cause opportunistic infections in selected patient groups. during the last decades it has emerged as a cause of chronic pulmonary infection in patients with cystic fibrosis (cf). m. abscessus strains exhibit either smooth or rough colony morphology. strains exhibiting the rough phenotype more often cause pulmonary infections in cf patients than did the smooth ones. here, we examined phagocytosis and production of cytokines by huma ... | 2013 | 23030647 |
| analysis of the genome of mycobacterium abscessus strain m94 reveals an uncommon cluster of trnas. | mycobacterium abscessus is a species of rapidly growing nontuberculous mycobacteria that is frequently associated with opportunistic infections in humans. here, we report the annotated genome sequence of m. abscessus strain m94, which showed an unusual cluster of trnas. | 2012 | 23012295 |
| genomic insights into the emerging human pathogen mycobacterium massiliense. | mycobacterium massiliense (mycobacterium abscessus group) is an emerging pathogen causing pulmonary disease and skin and soft tissue infections. we report the genome sequence of the type strain ccug 48898. | 2012 | 22965080 |
| progressive dyspnoea following the treatment of mycobacterium abscessus infection in an individual with relapsing granulamatosis with polyangitis (wegener's), complicated by hearing loss requiring cochlear implantation. | granulomatosis with polyangitis (wegener's) is a vasculitic disease predominantly affecting the lungs, skin, kidneys, ears, nose and throat. mycobacterium abscessus is an uncommon rapidly growing mycobacterium causing sporadic lung disease. this is the first report of both gpa and mycobacterium abscessus pulmonary disease reported in literature. | 2012 | 22947057 |
| inhaled therapies, azithromycin and mycobacterium abscessus in cystic fibrosis patients. | cystic fibrosis (cf) patients are at particularly high risk of developing lung disease caused by mycobacterium abscessus complex (mabsc). over the last 10 years, changes in cf treatment, with increasing use of inhaled therapies and low-dose azithromycin, have been accompanied by an increase in the prevalence of mabsc infections in cf patients. there is therefore some concern about the role of new cf treatments in the emergence of mabsc infections. we addressed this issue by means of a case-contr ... | 2013 | 22936714 |
| tender cutaneous nodules of the legs: diagnosis and clinical clues to diagnosis. | "tender cutaneous nodules of the legs" is a common manifestation in dermatology. histopathological investigation is usually required for this condition, because clinical data are frequently insufficient to make a definite diagnosis. | 2013 | 22928517 |
| clinical and microbiological differences between mycobacterium abscessus and mycobacterium massiliense lung diseases. | in recent years, many novel nontuberculous mycobacterial species have been discovered through genetic analysis. mycobacterium massiliense and m. bolletii have recently been identified as species separate from m. abscessus. however, little is known regarding their clinical and microbiological differences in japan. we performed a molecular identification of stored m. abscessus clinical isolates for further identification. we compared clinical characteristics, radiological findings, microbiological ... | 2012 | 22915613 |
| pulmonary disease caused by rapidly growing mycobacteria: a retrospective study of 44 cases in japan. | the features of pulmonary disease caused by rapidly growing mycobacteria (rgm) have not been sufficiently documented. | 2013 | 22890130 |
| genomic analysis of mycobacterium massiliense strain m115, an isolate from human sputum. | we report the draft genome sequence of a clinical isolate, strain m115, identified as mycobacterium massiliense, a member of the newly created taxon of mycobacterium abscessus subspecies bolletii comb. nov. | 2012 | 22887681 |
| therapeutic drug monitoring in the treatment of mycobacterium avium complex lung disease. | little is known regarding the application of therapeutic drug monitoring for treatment of mycobacterium avium complex (mac) lung disease. | 2012 | 22878282 |
| macrolide treatment for mycobacterium abscessus and mycobacterium massiliense infection and inducible resistance. | macrolides, such as clarithromycin (clr) and azithromycin (azm), are frequently the only oral antibiotics that are active against mycobacterium abscessus and m. massiliense infections. | 2012 | 22878281 |
| mycobacterium avium and mycobacterium abscessus complex target distinct cystic fibrosis patient subpopulations. | clinical observations suggest that mycobacterium avium complex (mac) and mycobacterium abscessus complex (mabsc) may affect cystic fibrosis (cf) patients with different characteristics and risk factors, but this has never been demonstrated within a single prospective cohort. | 2013 | 22857820 |
| acquisition of clarithromycin resistance mutations in the 23s rrna gene of mycobacterium abscessus in the presence of inducible erm(41). | antibiotic therapy of pulmonary mycobacterium abscessus infection is based on a combination treatment including clarithromycin. recent data demonstrated that m. abscessus may carry a chromosomal, inducible erm gene coding for the ribosomal methylase erm(41). the purpose of this study was to investigate whether in patients with chronic m. abscessus infection undergoing clarithromycin therapy, m. abscessus acquires clarithromycin resistance mutations in the rrl gene in addition to the presence of ... | 2012 | 22833642 |
| clinical significance of respiratory isolates for mycobacterium abscessus complex from pediatric patients. | mycobacterium abscessus complex is the most virulent of rapidly growing mycobacteria causing invasive lung disease. to better delineate clinical pediatric experience and outcomes with m. abscessus complex, we retrospectively gathered 5-year data on m. abscessus complex infection and outcomes in a large, hospital-based pediatric pulmonary center. patients were selected from the database of the microbiology department at miller children's hospital in long beach, ca. patients had at least one posit ... | 2013 | 22833551 |
| molecular typing of mycobacterium abscessus based on tandem-repeat polymorphism. | a variable-number tandem-repeat (vntr) typing assay for the differentiation of mycobacterium abscessus strains was developed. this assay showed complete reproducibility, locus stability, and a discriminatory power (hunter-gaston discriminatory index [hgdi] of 0.9563) that is superior to that of multilocus sequencing. it is a promising tool for the investigation of mycobacterium abscessus epidemiology and nosocomial outbreaks. | 2012 | 22760048 |
| non-tuberculous mycobacterial infection among lung transplant recipients: a 15-year cohort study. | the incidence of infection with non-tuberculous mycobacteria (ntm) after lung transplant is insufficiently defined. data on the impact of ntm infection on lung transplant survival are conflicting. | 2012 | 22676720 |
| genome sequence of the mycobacterium abscessus strain m93. | mycobacterium abscessus is a rapid-growing species of nontuberculous mycobacteria that is frequently associated with opportunistic infections in humans. we report herein the draft genome sequence of m. abscessus strain m93. | 2012 | 22628507 |
| first case report of chronic pulmonary lung disease caused by mycobacterium abscessus in two immunocompetent patients in saudi arabia. | mycobacterium abscessus is a rapidly growing non-tuberculous mycobacterium that is a common water contaminant, and an exclusive opportunistic pathogen in immunosuppressed patients; however, it occasionally causes lung infections in immunocompetent persons as well. we report two cases of chronic pulmonary infection caused by m abscessus in two young migrant workers; they were nonreactive to hiv antigen, without any history of pulmonary disorders and no evidence of any immunocompromized status. bo ... | 2012 | 22588446 |
| activities of moxifloxacin in combination with macrolides against clinical isolates of mycobacterium abscessus and mycobacterium massiliense. | infections caused by mycobacterium abscessus and mycobacterium massiliense are on the rise among humans. although macrolides, including clarithromycin (clr) and azithromycin (azm), are key antibiotics for the treatment of m. abscessus and m. massiliense infections, treatment regimens for these infections are still largely undefined. in this study, we evaluated the in vitro, ex vivo, and in vivo activities of moxifloxacin (mxf) in combination with macrolides against clinically isolated m. abscess ... | 2012 | 22564831 |
| non-tuberculous mycobacteria infection: 75 cases. | non-tuberculosis mycobacterium is especially seen in aids and non-immunosuppressant patients. this study was designed to evaluate data relating to non-tuberculosis mycobacterium content in patients' sputum for the clinical importance. | 2012 | 22554363 |
| disseminated mycobacterium immunogenum infection presenting with septic shock and skin lesions in a renal transplant recipient. | mycobacterium immunogenum is a relatively new species within the mycobacterium chelonae-mycobacterium abscessus group of rapidly growing mycobacteria (rgm). m. immunogenum was first characterized in 2001 and, similar to other rgm, is an ubiquitous environmental organism. this organism has most commonly been implicated in cutaneous infection in both healthy and immunosuppressed patients. to our knowledge, this is the first reported case of septic shock in the setting of disseminated m. immunogenu ... | 2012 | 22548769 |
| resistance mechanisms and drug susceptibility testing of nontuberculous mycobacteria. | nontuberculous mycobacteria (ntm) are increasingly recognized as causative agents of opportunistic infections in humans. for most ntm infections the therapy of choice is drug treatment, but treatment regimens differ by species, in particular between slow (e.g. mycobacterium avium complex, mycobacterium kansasii) and rapid growers (e.g. mycobacterium abscessus, mycobacterium fortuitum). in general, drug treatment is long, costly, and often associated with drug-related toxicities; outcome of drug ... | 2012 | 22525524 |
| the use of rpob sequence analysis in the differentiation of mycobacterium abscessus and mycobacterium chelonae: a critical judgement in cystic fibrosis? | individuals suffering from fibrocystic disease may acquire non-tuberculous mycobacteria as colonizing or infecting organisms. mycobacterium abscessus is of particular concern because it may be very difficult to eradicate and may mitigate against lung transplantation. however, this species may be difficult to reliably differentiate from the closely related m. chelonae. we have developed a rapid, low-cost, short sequence-based technique to confirm species identity by analysis of a segment of the r ... | 2012 | 22429487 |