Publications

TitleAbstractYear
Filter
PMID(sorted ascending)
Filter
conditional gene expression in mycobacterium abscessus.mycobacterium abscessus is an emerging human pathogen responsible for lung infections, skin and soft-tissue infections and disseminated infections in immunocompromised patients. it may exist either as a smooth (s) or rough (r) morphotype, the latter being associated with increased pathogenicity in various models. genetic tools for homologous recombination and conditional gene expression are desperately needed to allow the study of m. abscessus virulence. however, descriptions of knock-out (ko) m ...201122195042
Comparative study with two different enrichments in the culture media used in the disinfectant efficacy assay.Recent changes in Brazilian legislation for commercial disinfectants have been published due to the recent epidemic of nosocomial infections caused by rapidly growing mycobacteria (RGM) in many states of Brazil over the last 8years. One of these documents requires that all the manufacturers provide evidence of efficacy of sterilizing and disinfectant products, used for semi critical medical devices, against the Mycobacterium bovis BCG Moreau and Mycobacterium abscessus subsp. bolletii INCQS 0059 ...201122197720
Whole-Genome Sequence of the Emerging Pathogen Mycobacterium abscessus Strain 47J26.Mycobacterium abscessus is a rapidly growing environmental mycobacterium commonly found in soil and water which is often also associated with infections in humans, particularly of the lung. We report herein the draft genome sequence of M. abscessus strain 47J26.201222207751
Antibacterial activities of dendritic amphiphiles against nontuberculous mycobacteria.The anti-mycobacterial activities of nine series of dicarboxyl and tricarboxyl dendritic amphiphiles with one alkyl, two alkyl, and cholestanyl tails against Mycobacterium abscessus, Mycobacterium avium, Mycobacterium chelonae, Mycobacterium marinum and Mycobacterium smegmatis have been measured. The dendritic amphiphiles overcame the limited aqueous solubility of natural long-chain fatty acids, alcohols, and amines to enable profiling the susceptibilities of the different mycobacterial species ...201122209468
mycobacterium abscessus glycopeptidolipid prevents respiratory epithelial tlr2 signaling as measured by hβd2 gene expression and il-8 release.mycobacterium abscessus has emerged as an important cause of lung infection, particularly in patients with bronchiectasis. innate immune responses must be highly effective at preventing infection with m. abscessus because it is a ubiquitous environmental saprophyte and normal hosts are not commonly infected. m. abscessus exists as either a glycopeptidolipid (gpl) expressing variant (smooth phenotype) in which gpl masks underlying bioactive cell wall lipids, or as a variant lacking gpl which is i ...201122216191
the use of a two-gene sequencing approach to accurately distinguish between the species within the mycobacterium abscessus complex and mycobacterium chelonae.mycobacterium abscessus [m. abscessus (sensu lato) or m. abscessus complex] comprises three closely related species: m. abscessus (sensu stricto), hereafter referred to as m. abscessus, m. bolletii and m. massiliense. we describe here an accurate and robust method for distinguishing m. chelonae from m. abscessus, m. bolletii and m. massiliense, using polymerase chain reaction (pcr) and the sequencing of house-keeping gene targets (hsp65 and rpob). sequencing of the soda gene is of little additio ...201222222989
molecular identification and typing of mycobacterium massiliense isolated from postsurgical infections in brazil.one hundred thirty-one cases of postsurgical infections were reported in southern region of brazil between august 2007 and january 2008. thirty-nine (29.8%) cases were studied; this report describes epidemiological findings, species identification, antimicrobial susceptibility and clonal diversity of rapidly growing mycobacteria isolated in this outbreak.201122230849
hsp65 pcr-restriction analysis (pra) with capillary electrophoresis for species identification and differentiation of mycobacterium kansasii and mycobacterium chelonae-mycobacterium abscessus group.the aim of the present study was to identify and differentiate mycobacterium kansasii and mycobacterium chelonae-mycobacterium abscessus group strains isolated from clinical and environmental sources in different countries.201222240012
respiratory outbreak of mycobacterium abscessus subspecies massiliense in a lung transplant and cystic fibrosis center. 201222246710
mycobacterium abscessus granulomatous prostatitis.infectious granulomatous prostatitis is uncommon, and most cases of granulomatous prostatitis are classified as nonspecific granulomatous prostatitis. from 2007 to 2009, 5 patients experienced poor wound healing after radical prostatectomy for prostate cancer at a specialist cancer center. mycobacterium abscessus was cultured from the debridement specimens, and acid-fast-positive bacilli were identified histologically within the prostates. all 180 radical prostatectomy specimens from may 2007 to ...201222261705
mycobacterium abscessus: a new antibiotic nightmare.the intrinsic and acquired resistance of mycobacterium abscessus to commonly used antibiotics limits the chemotherapeutic options for infections caused by these mycobacteria. intrinsic resistance is attributed to a combination of the permeability barrier of the complex multilayer cell envelope, drug export systems, antibiotic targets with low affinity and enzymes that neutralize antibiotics in the cytoplasm. to date, acquired resistance has only been observed for aminoglycosides and macrolides, ...201222290346
atypical mycobacterium detection in refractory chronic rhinosinusitis.atypical mycobacterium (am) involvement in refractory chronic rhinosinusitis (crs) is sought by some surgeons with customary acid-fast bacilli cultures (afbc) in the operating room (or). we evaluate our experience with am in crs by describing (1) associated risk factors, (2) species identified, and (3) frequency of positive cultures in clinic versus or.201222301103
case series of mycobacterium abscessus infections associated with a trigger point injection and epidural block at a rural clinic.the aim of this report is to investigate mycobacterium abscessus infections at a rural clinic and carry out a surveillance program to determine the extent and source of these infections.201222323979
risk factors for mycobacterium abscessus infection in cystic fibrosis patients; a case-control study.mycobacterium abscessus is a nontuberculous mycobacterium that is increasingly recognized as an opportunistic pathogen in cystic fibrosis (cf) patients. factors that predispose cf patients to infection by this environmental bacterium remain unknown. in a case-control study of 22 cf patients with m. abscessus infection and 22 cf controls, we investigated risk factors for mab infection as well as the positioning of mab infection in relation to the other pathogens infecting cf patients. no clear ri ...201222342403
antimicrobial susceptibility of nontuberculous mycobacteria from eye infections.to characterize formerly designated atypical or nontuberculous mycobacteria (ntm) associated with eye infections.201222362004
pulmonary mycobacterium abscessus: a canary in the cystic fibrosis coalmine.we present a case of pulmonary nontuberculous mycobacterial infection (pntm) with m. abscessus. after exclusion of genetic immune disorders known to cause ntm susceptibility, we found compound heterozygosity of two mutations, f508del and r117h in cftr. the combination of f508del with a hypomorphic cftr mutation can cause a mild cystic fibrosis (cf) phenotype with delayed cf symptoms in adulthood. although the patient was continuously treated for her lung infection by different physicians for mor ...201222366207
serial ct findings of mycobacterium massiliense pulmonary disease compared with mycobacterium abscessus disease after treatment with antibiotic therapy.to present the serial computed tomographic (ct) findings of lung abnormalities in mycobacterium massiliense pulmonary disease compared with those in mycobacterium abscessus disease.201222371609
evaluation of antimicrobial susceptibilities of rapidly growing mycobacteria by sensititre rapmyco panel.this study used sensititre rapmyco to test the activities of amikacin, cefoxitin, ciprofloxacin, clarithromycin, doxycycline, imipenem, linezolid, sulfamehoxazole, tigecycline and tobramycin against 25 clinical isolates of rapidly growing mycobacteria (rgm), including the common disease producing species mycobacterium abscessus, mycobacterium chelonae, mycobacterium fortuitum and mycobacterium peregrinum. analysis of the four different rgm species showed that isolates of m. fortuitum and m. pere ...201222378556
molecular fingerprinting of mycobacterium abscessus strains in a cohort of pediatric cystic fibrosis patients.forty-one mycobacterium abscessus complex isolates from 17 pediatric cystic fibrosis (cf) patients were typed using a novel variable-number tandem repeat (vntr) scheme and an automated repetitive-pcr (rep-pcr) system. both vntr and rep-pcr typing methods differentiate between members of the m. abscessus complex. the isolates from individual patients are indistinguishable, and the data strongly suggest that individual cf patients are persistently infected with one strain and also suggests that di ...201222403419
the use of rpob sequence analysis in the differentiation of mycobacterium abscessus and mycobacterium chelonae: a critical judgement in cystic fibrosis?individuals suffering from fibrocystic disease may acquire non-tuberculous mycobacteria as colonizing or infecting organisms. mycobacterium abscessus is of particular concern because it may be very difficult to eradicate and may mitigate against lung transplantation. however, this species may be difficult to reliably differentiate from the closely related m. chelonae. we have developed a rapid, low-cost, short sequence-based technique to confirm species identity by analysis of a segment of the r ...201222429487
the surface lipids of non-tuberculous mycobacteria suppress production of phagocyte activating cytokines in human peripheral blood mononuclear cells.the genus mycobacterium includes obligate pathogens as well as opportunistic and non-pathogenic species ubiquitous in the environment. mycobacteria have a unique cell wall abundant in lipids. here we investigated cytokine production by human peripheral blood mononuclear cells (pbmc) in response to the opportunistic mycobacteria mycobacterium avium and mycobacterium abscessus, the non-pathogenic mycobacterium gordonae and extracted surface lipids from the three species. the cytokine response elic ...201222497872
resistance mechanisms and drug susceptibility testing of nontuberculous mycobacteria.nontuberculous mycobacteria (ntm) are increasingly recognized as causative agents of opportunistic infections in humans. for most ntm infections the therapy of choice is drug treatment, but treatment regimens differ by species, in particular between slow (e.g. mycobacterium avium complex, mycobacterium kansasii) and rapid growers (e.g. mycobacterium abscessus, mycobacterium fortuitum). in general, drug treatment is long, costly, and often associated with drug-related toxicities; outcome of drug ...201222525524
draft genome sequence of mycobacterium abscessus subsp. bolletii bd(t).mycobacterium abscessus subsp. bolletii is an increasing cause of human pulmonary disease and infections of the skin and soft tissues. consistent reports of human infections indicate that m. bolletii is a highly pathogenic, emerging species of rapidly growing mycobacteria (rgm). here we report the first whole-genome sequence of m. abscessus subsp. bolletii bd(t).201222535937
disseminated mycobacterium immunogenum infection presenting with septic shock and skin lesions in a renal transplant recipient.mycobacterium immunogenum is a relatively new species within the mycobacterium chelonae-mycobacterium abscessus group of rapidly growing mycobacteria (rgm). m. immunogenum was first characterized in 2001 and, similar to other rgm, is an ubiquitous environmental organism. this organism has most commonly been implicated in cutaneous infection in both healthy and immunosuppressed patients. to our knowledge, this is the first reported case of septic shock in the setting of disseminated m. immunogenu ...201222548769
non-tuberculous mycobacteria infection: 75 cases.non-tuberculosis mycobacterium is especially seen in aids and non-immunosuppressant patients. this study was designed to evaluate data relating to non-tuberculosis mycobacterium content in patients' sputum for the clinical importance.201222554363
activities of moxifloxacin in combination with macrolides against clinical isolates of mycobacterium abscessus and mycobacterium massiliense.infections caused by mycobacterium abscessus and mycobacterium massiliense are on the rise among humans. although macrolides, including clarithromycin (clr) and azithromycin (azm), are key antibiotics for the treatment of m. abscessus and m. massiliense infections, treatment regimens for these infections are still largely undefined. in this study, we evaluated the in vitro, ex vivo, and in vivo activities of moxifloxacin (mxf) in combination with macrolides against clinically isolated m. abscess ...201222564831
in vitro activity of tp-271 against mycobacterium abscessus, mycobacterium fortuitum, and nocardia species.the in vitro activities of tp-271, a novel fluorocycline antimicrobial, against 22 isolates of mycobacterium abscessus, 22 isolates of mycobacterium fortuitum, and 19 isolates of nocardia spp. were studied by a microtiter broth dilution method. the mic(90)s for m. abscessus, m. fortuitum, and nocardia spp. were 0.5 μg/ml, 0.03 μg/ml, and 8 μg/ml, respectively. tp-271 was significantly more active than the respective control drug in virtually all tests.201222585214
first case report of chronic pulmonary lung disease caused by mycobacterium abscessus in two immunocompetent patients in saudi arabia.mycobacterium abscessus is a rapidly growing non-tuberculous mycobacterium that is a common water contaminant, and an exclusive opportunistic pathogen in immunosuppressed patients; however, it occasionally causes lung infections in immunocompetent persons as well. we report two cases of chronic pulmonary infection caused by m abscessus in two young migrant workers; they were nonreactive to hiv antigen, without any history of pulmonary disorders and no evidence of any immunocompromized status. bo ...201222588446
genome sequence of the mycobacterium abscessus strain m93.mycobacterium abscessus is a rapid-growing species of nontuberculous mycobacteria that is frequently associated with opportunistic infections in humans. we report herein the draft genome sequence of m. abscessus strain m93.201222628507
non-tuberculous mycobacterial infection among lung transplant recipients: a 15-year cohort study.the incidence of infection with non-tuberculous mycobacteria (ntm) after lung transplant is insufficiently defined. data on the impact of ntm infection on lung transplant survival are conflicting.201222676720
the antibiotic resistance arrow of time: efflux pump induction is a general first step in the evolution of mycobacterial drug resistance.we hypothesize that low-level efflux pump expression is the first step in the development of high-level drug resistance in mycobacteria. we performed 28-day azithromycin dose-effect and dose-scheduling studies in our hollow-fiber model of disseminated mycobacterium avium-m. intracellulare complex. both microbial kill and resistance emergence were most closely linked to the within-macrophage area under the concentration-time curve (auc)/mic ratio. quantitative pcr revealed that subtherapeutic azi ...201222751536
molecular typing of mycobacterium abscessus based on tandem-repeat polymorphism.a variable-number tandem-repeat (vntr) typing assay for the differentiation of mycobacterium abscessus strains was developed. this assay showed complete reproducibility, locus stability, and a discriminatory power (hunter-gaston discriminatory index [hgdi] of 0.9563) that is superior to that of multilocus sequencing. it is a promising tool for the investigation of mycobacterium abscessus epidemiology and nosocomial outbreaks.201222760048
an unusual surgical site infection in a liver transplant recipient.mycobacterium abscessus is a rare cause of human infection and is difficult to diagnose unless it is suspected. a 45-year-old woman underwent deceased donor liver transplantation following which she developed non-healing surgical site infection, which did not resolve with routine antibiotics for 2 months. the scraping of the wound revealed m abscessus infection. definitive identification of this species of mycobacterium was possible by its growth characteristics on culture and reverse line blot ...201022778369
clinical significance of respiratory isolates for mycobacterium abscessus complex from pediatric patients.mycobacterium abscessus complex is the most virulent of rapidly growing mycobacteria causing invasive lung disease. to better delineate clinical pediatric experience and outcomes with m. abscessus complex, we retrospectively gathered 5-year data on m. abscessus complex infection and outcomes in a large, hospital-based pediatric pulmonary center. patients were selected from the database of the microbiology department at miller children's hospital in long beach, ca. patients had at least one posit ...201322833551
acquisition of clarithromycin resistance mutations in the 23s rrna gene of mycobacterium abscessus in the presence of inducible erm(41).antibiotic therapy of pulmonary mycobacterium abscessus infection is based on a combination treatment including clarithromycin. recent data demonstrated that m. abscessus may carry a chromosomal, inducible erm gene coding for the ribosomal methylase erm(41). the purpose of this study was to investigate whether in patients with chronic m. abscessus infection undergoing clarithromycin therapy, m. abscessus acquires clarithromycin resistance mutations in the rrl gene in addition to the presence of ...201222833642
mycobacterium avium and mycobacterium abscessus complex target distinct cystic fibrosis patient subpopulations.clinical observations suggest that mycobacterium avium complex (mac) and mycobacterium abscessus complex (mabsc) may affect cystic fibrosis (cf) patients with different characteristics and risk factors, but this has never been demonstrated within a single prospective cohort.201322857820
macrolide treatment for mycobacterium abscessus and mycobacterium massiliense infection and inducible resistance.macrolides, such as clarithromycin (clr) and azithromycin (azm), are frequently the only oral antibiotics that are active against mycobacterium abscessus and m. massiliense infections.201222878281
therapeutic drug monitoring in the treatment of mycobacterium avium complex lung disease.little is known regarding the application of therapeutic drug monitoring for treatment of mycobacterium avium complex (mac) lung disease.201222878282
annotated genome sequence of mycobacterium massiliense strain m154, belonging to the recently created taxon mycobacterium abscessus subsp. bolletii comb. nov.mycobacterium massiliense has recently been proposed as a member of mycobacterium abscessus subsp. bolletii comb. nov. strain m154, a clinical isolate from the bronchoalveolar lavage fluid of a malaysian patient presenting with lower respiratory tract infection, was subjected to shotgun dna sequencing with the illumina sequencing technology to obtain whole-genome sequence data for comparison with other genetically related strains within the m. abscessus species complex.201222887675
genomic analysis of mycobacterium massiliense strain m115, an isolate from human sputum.we report the draft genome sequence of a clinical isolate, strain m115, identified as mycobacterium massiliense, a member of the newly created taxon of mycobacterium abscessus subspecies bolletii comb. nov.201222887681
pulmonary disease caused by rapidly growing mycobacteria: a retrospective study of 44 cases in japan.the features of pulmonary disease caused by rapidly growing mycobacteria (rgm) have not been sufficiently documented.201322890130
clinical and microbiological differences between mycobacterium abscessus and mycobacterium massiliense lung diseases.in recent years, many novel nontuberculous mycobacterial species have been discovered through genetic analysis. mycobacterium massiliense and m. bolletii have recently been identified as species separate from m. abscessus. however, little is known regarding their clinical and microbiological differences in japan. we performed a molecular identification of stored m. abscessus clinical isolates for further identification. we compared clinical characteristics, radiological findings, microbiological ...201222915613
tender cutaneous nodules of the legs: diagnosis and clinical clues to diagnosis."tender cutaneous nodules of the legs" is a common manifestation in dermatology. histopathological investigation is usually required for this condition, because clinical data are frequently insufficient to make a definite diagnosis.201322928517
otitis media and otomastoiditis caused by mycobacterium massiliense (mycobacterium abscessus subsp. bolletii).we describe two patients with otologic infections caused by mycobacterium massiliense (m. abscessus subsp. bolletti) which were identified using erm(41) pcr, 23s rrna, and rpob gene sequence analysis. they were middle-aged adults with underlying otologic diseases and were treated successfully with clarithromycin-based combination regimens for 3 and 9 months, respectively.201222933592
inhaled therapies, azithromycin and mycobacterium abscessus in cystic fibrosis patients.cystic fibrosis (cf) patients are at particularly high risk of developing lung disease caused by mycobacterium abscessus complex (mabsc). over the last 10 years, changes in cf treatment, with increasing use of inhaled therapies and low-dose azithromycin, have been accompanied by an increase in the prevalence of mabsc infections in cf patients. there is therefore some concern about the role of new cf treatments in the emergence of mabsc infections. we addressed this issue by means of a case-contr ...201322936714
non-tuberculous mycobacteria and their surface lipids efficiently induced il-17 production in human t cells.interleukin-17 (il-17) is produced by a subset of cd4(+) t helper (th) lymphocytes known as th17 cells. in humans, il-1β, enhanced by il-6 and il-23 is crucial for differentiation of these cells. il-17 evokes inflammation and is involved in host defence against microorganisms, although little is known about its role in diseases caused by non-tuberculous mycobacteria. the genus mycobacterium contains both obligate and opportunistic pathogens as well as saprophytes, and the mycobacterial cell enve ...201222939918
progressive dyspnoea following the treatment of mycobacterium abscessus infection in an individual with relapsing granulamatosis with polyangitis (wegener's), complicated by hearing loss requiring cochlear implantation.granulomatosis with polyangitis (wegener's) is a vasculitic disease predominantly affecting the lungs, skin, kidneys, ears, nose and throat. mycobacterium abscessus is an uncommon rapidly growing mycobacterium causing sporadic lung disease. this is the first report of both gpa and mycobacterium abscessus pulmonary disease reported in literature.201222947057
probable disseminated mycobacterium abscessus subspecies bolletii infection in a patient with idiopathic cd4+ t lymphocytopenia: a case report. 201222947084
genomic insights into the emerging human pathogen mycobacterium massiliense.mycobacterium massiliense (mycobacterium abscessus group) is an emerging pathogen causing pulmonary disease and skin and soft tissue infections. we report the genome sequence of the type strain ccug 48898.201222965080
analysis of the genome of mycobacterium abscessus strain m94 reveals an uncommon cluster of trnas.mycobacterium abscessus is a species of rapidly growing nontuberculous mycobacteria that is frequently associated with opportunistic infections in humans. here, we report the annotated genome sequence of m. abscessus strain m94, which showed an unusual cluster of trnas.201223012295
phagocytosis and cytokine response to rough and smooth colony variants of mycobacterium abscessus by human peripheral blood mononuclear cells.mycobacterium abscessus is a non-tuberculous mycobacteria able to cause opportunistic infections in selected patient groups. during the last decades it has emerged as a cause of chronic pulmonary infection in patients with cystic fibrosis (cf). m. abscessus strains exhibit either smooth or rough colony morphology. strains exhibiting the rough phenotype more often cause pulmonary infections in cf patients than did the smooth ones. here, we examined phagocytosis and production of cytokines by huma ...201323030647
impact of molecular diagnosis on treating mendelian susceptibility to mycobacterial diseases.the il-12-ifn-γ axis is critical for immune defense against mycobacterial infections. inherited mutations that affect normal activation of this self-amplifying cytokine reaction lead to increased chances of mycobacterial infections, known as mendelian susceptibility to mycobacterial diseases (msmd). delayed diagnosis and difficulty in identifying pathogenic mycobacteria hinder proper treatment of patients, so the aim of this study was to facilitate the diagnosis of mycobacterial infections in ms ...201223036270
genomic analysis of mycobacterium abscessus strain m139, which has an ambiguous subspecies taxonomic position.mycobacterium abscessus is a ubiquitous, rapidly growing species of nontuberculous mycobacteria that colonizes organic surfaces and is frequently associated with opportunistic infections in humans. we report here the draft genome sequence of mycobacterium abscessus strain m139, which shows genomic features reported to be characteristic of both mycobacterium abscessus subsp. abscessus and mycobacterium abscessus subsp. massiliense.201223045507
mycobacterium abscessus lung disease in a patient with previous pulmonary tuberculosis.patients with pre-existing lung damage, such as due to pulmonary tuberculosis (ptb), are susceptible to nontuberculous mycobacteria (ntm) infections. for patients with previous ptb, it is difficult to differentiate ntm lung disease from ptb, especially in tuberculosis (tb) high-burdened countries. here, we report a case of mycobacterium abscessus lung disease with a previous history of ptb. the patient underwent a right upper lobe lobectomy due to disappointing treatment response with anti-tuber ...201223077819
patients with inhibitory and neutralizing auto-antibodies to interferon-γ resemble the sporadic adult-onset phenotype of mendelian susceptibility to mycobacterial disease (msmd) lacking bacille calmette-guerin (bcg)-induced diseases.to recognize patients with inhibitory and neutralizing auto-antibodies to interferon-γ (autoabs-ifn-γ) presenting with the sporadic phenotype of mendelian susceptibility to mycobacterial disease (msmd) mainly characterized by recurrent intracellular mycobacterium or/and salmonella infections, we comprehensively investigated il12/23-ifn-γ signaling, candidate genetic sequencings or/and protein expressions of il12rb1, ifnrg1, il12p40, ifnrg2, stat1, ikka, nemo, cybb and irf8 in four patients. thei ...201323083630
treatment of mycobacterium abscessus: all macrolides are equal, but perhaps some are more equal than others. 201223118083
comparative drug resistance of mycobacterium abscessus and m. chelonae isolates from patients with and without cystic fibrosis in the united kingdom.the isolation of rapidly growing mycobacteria (rgm), particularly mycobacterium abscessus, from individuals with cystic fibrosis (cf) is associated with poor clinical outcome due to broad drug resistance and the difficulty of eradicating the organisms. susceptibility testing is recommended to guide therapy. a disc diffusion method is used in the united kingdom, whereas in the united states, the clsi (clinical and laboratory standards institute) recommends the broth dilution method. the purpose o ...201323135941
genome sequence of mycobacterium abscessus strain m152.mycobacterium abscessus is an environmental bacterium with increasing clinical relevance. here, we report the annotated whole-genome sequence of m. abscessus strain m152.201223144407
pulmonary sequestration infected with nontuberculous mycobacteria: a report of two cases and literature review.we report two cases of pulmonary sequestration infected with nontuberculous mycobacteria (ntm): mycobacterium avium and mycobacterium abscessus. chest computed tomography showed pneumonic consolidation in the right lower lobe, which received a systemic blood supply from the descending aorta in both patients. video-assisted thoracoscopic surgeries were successfully performed and pathological examinations revealed multiple caseating granulomas. a review of the literature revealed only seven previo ...201223146810
[comparison of clinical manifestations between mycobacterium avium-intracellulare complex and mycobacterium abscessus pulmonary diseases].to compare the clinical manifestations of nontuberculous mycobacterial (ntm) pulmonary diseases caused by mycobacterium avium-intracellulare complex (mac) and mycobacterium abscessus.201223158006
endovascular mycobacterium abscessus infection in a heart transplant recipient: a case report and review of the literature.nontuberculous mycobacteria are ubiquitous in the environment. although rarely a cause of infection in immunocompetent individuals, increased risk and severity of infection are seen in patients who are immunocompromised, such as those with solid organ transplants. in this report, we describe the first case of disseminated endovascular mycobacterium abscessus in a heart transplant recipient. a review of the literature regarding this infection in heart transplant recipients and its therapeutic opt ...201323176090
increased incidence of cutaneous nontuberculous mycobacterial infection, 1980 to 2009: a population-based study.to determine the incidence and clinical characteristics of cutaneous nontuberculous mycobacterial (ntm) infection during the past 30 years and whether the predominant species have changed.201323218797
impaired expression of mapk is associated with the downregulation of tnf-α, il-6, and il-10 in mycobacterium abscessus lung disease.healthy individuals who develop nontuberculous mycobacteria (ntm) lung disease are likely to have specific susceptibility factors which can lead to a ntm infection. the aim of the present study was to investigate the mechanism underlying innate immune responses, including the role of mitogen-activated protein kinase (mapk), in mycobacterium abscessus lung disease.201223227067
antibiotic treatment for nontuberculous mycobacteria lung infection in people with cystic fibrosis.nontuberculous mycobacteria are mycobacteria, other than those in the mycobacterium tuberculosis complex, and are commonly found in the environment. nontuberculous mycobacteria species (most commonly mycobacterium avium complex and mycobacterium abscessus) are isolated from the respiratory tract of approximately 5% to 20% of individuals with cystic fibrosis; they can cause lung disease in people with cystic fibrosis leading to more a rapid decline in lung function and even death in certain circu ...201223235684
catheter-related mycobacterium abscessus bacteremia manifested with skin nodules, pneumonia, and mediastinal lymphadenopathy.although previously rare, catheter-related bloodstream infection caused by rapidly growing mycobacteria is now increasingly encountered, especially among cancer patients who have catheters implanted for chemotherapy treatments. a 73-year-old female patient with acute myeloid leukemia (aml) had mycobacterium abscessus bacteremia with manifestations of multiple skin nodules, pneumonia, and mediastinal lymphadenopathy 4 months after the implantation of a peripherally inserted central catheter (picc ...201323257257
environmental effects on protection against mycobacterium tuberculosis after immunization with ad85a.previously we have shown that intradermal (i.d.) immunization with a recombinant adenovirus expressing antigen 85a (ad85a) induced a strong splenic cd8t cell response in balb/c mice but a weak lung immune response and did not protect mice against challenge with mycobacterium tuberculosis (mtb). after moving to a new animal house, the same i.d. immunization induced a strong lung immune response and the mice were protected against mtb challenge. increased numbers of antigen 85a-specific cd8 cells ...201223262169
successful treatment with chemotherapy and corticosteroids of pulmonary mycobacterium abscessus infection accompanied by pleural effusion.we describe a 50-year-old woman with rapidly progressive pulmonary mycobacterium abscessus (m. abscessus) infection accompanied by pleural effusion and organizing pneumonia (op). ct scan showed consolidation, centrilobular shadows, ground-glass opacity (ggo), and cavities. a transbronchial lung biopsy showed nonnecrotizing granuloma surrounded by infiltrative lymphocyte-dominant inflammatory cells, and lymphocytes in bronchoalveolar lavage fluid (balf) were increased. we considered op occurred s ...201223263189
nontuberculous mycobacterial disease is not a contraindication to lung transplantation in patients with cystic fibrosis: a retrospective analysis in a danish patient population.whether nontuberculous mycobacterial (ntm) disease is a contraindication to lung transplantation remains controversial. we conducted a nationwide study to evaluate the clinical importance of ntm infection among lung transplant patients with cystic fibrosis (cf) in denmark and to determine if ntm infection poses a contraindication to lung transplantation. all cf patients with current or prior ntm who had undergone lung transplantation were identified. out of 52 lung transplant patients with cf 9 ...201223267788
mycobacterium abscessus pneumonia in an atlantic bottlenose dolphin (tursiops truncatus).mycobacterium abscessus pneumonia was diagnosed antemortem in a 23-yr-old male atlantic bottlenose dolphin (tursiops truncatus). clinical signs included lethargy, hyporexia, coughing, and bloody respiratory discharge. diagnostic findings included neutrophilic leukocytosis, anemia, elevated erythrocyte sedimentation rate, and repeated forceful exhaled breath (sputum) cytology, with acute inflammatory cells and acid-fast positive beaded rods. the bacteria were initially identified free in the sput ...201223272373
mycobacterium abscessus multispacer sequence typing.mycobacterium abscessus group includes antibiotic-resistant, opportunistic mycobacteria that are responsible for sporadic cases and outbreaks of cutaneous, pulmonary and disseminated infections. however, because of their close genetic relationships, accurate discrimination between the various strains of these mycobacteria remains difficult. in this report, we describe the development of a multispacer sequence typing (mst) analysis for the simultaneous identification and typing of m. abscessus my ...201323294800
rapid tests for the detection of the mycobacterium abscessus subsp. bolletii strain responsible for an epidemic of surgical-site infections in brazil.a single strain of mycobacterium abscessus subsp. bolletii, characterised by a particular rpob sequevar and two highly related pulsed field gel electrophoresis patterns has been responsible for a nationwide outbreak of surgical infections in brazil since 2004. in this study, we developed molecular tests based on polymerase chain reaction restriction-enzyme analysis (pra) and sequencing for the rapid identification of this strain. sequences of 15 dna regions conserved in mycobacteria were retriev ...201223295745
synergistic activities of tigecycline with clarithromycin or amikacin against rapidly growing mycobacteria in taiwan.the occurrence of diseases caused by rapidly growing mycobacteria (rgm) is increasing in taiwan. in this study, the in vitro antimicrobial activities of tigecycline, minocycline, tetracycline and doxycycline were evaluated against 160 clinical rgm isolates, including 34 mycobacterium abscessus sensu stricto (s.s.), 44 mycobacterium massiliense, 1 mycobacterium bolletii, 58 mycobacterium fortuitum and 23 mycobacterium chelonae. clarithromycin and amikacin were tested alone as well as for synergis ...201323312605
mycobacteriosis in zebrafish colonies.mycobacteriosis, a chronic bacterial infection, has been associated with severe losses in some zebrafish facilities and low-level mortalities and unknown impacts in others. the occurrence of at least six different described species (mycobacterium abscessus, m. chelonae, m. fortuitum, m. haemophilum, m. marinum, m. peregrinum) from zebrafish complicates diagnosis and control because each species is unique. as a generalization, mycobacteria are often considered opportunists, but m. haemophilum and ...201223382341
cystic fibrosis cfbe41o- cells contain tlr1 snp i602s and fail to respond to mycobacterium abscessus.mycobacterium abscessus causes lung infection in patients with cystic fibrosis. m. abscessus stimulates the host innate immune response via tlr2 on respiratory epithelial cells. signaling through tlr2 requires the formation of tlr2/tlr1 heterodimers on the cell surface.201323403223
whole-genome shotgun sequencing of mycobacterium abscessus m156, an emerging clinical pathogen in malaysia.mycobacterium abscessus is an emerging clinical pathogen commonly associated with non-tuberculous mycobacterial infections. we report herein the draft genome of m. abscessus strain m156.201323405341
pulmonary mycobacterium abscessus disease in a patient receiving low-dose methotrexate for treatment of early rheumatoid arthritis.a 70-year-old woman with methotrexate (mtx)-refractory rheumatoid arthritis (ra) was referred to our hospital for introduction of biological therapy. on high-resolution computed tomography scans, the patient exhibited abnormal findings such as bronchiectasis and centrilobular small nodules, which were highly suggestive of pulmonary nontuberculous mycobacterial (ntm) disease. although mycobacterial cultures of sputum specimens yielded negative results, cultures of bronchoalveolar lavage fluids gr ...201323430370
mycobacterium abscessus induces a limited pattern of neutrophil activation that promotes pathogen survival.mycobacterium abscessus is a rapidly growing mycobacterium increasingly detected in the neutrophil-rich environment of inflamed tissues, including the cystic fibrosis airway. studies of the immune reaction to m. abscessus have focused primarily on macrophages and epithelial cells, but little is known regarding the neutrophil response despite the predominantly neutrophillic inflammation typical of these infections. in the current study, human neutrophils released less superoxide anion in response ...201323451220
mycobacterium abscessus complex lung infection in a toddler with a tracheostomy.mycobacterium abscessus complex and other non-tuberculous mycobacteria are infrequently encountered respiratory pathogens in patients with tracheostomies. we report a 4-year-old girl with a tracheostomy, placed during infancy for management of severe bronchopulmonary dysplasia and laryngeal stenosis, who developed a m. abscessus complex lung infection. there was clear evidence of parenchymal involvement and true infection beyond colonization. she demonstrated dramatic clinical, laboratory, and r ...201423460506
mycobacterial genotypes are associated with clinical manifestation and progression of lung disease caused by mycobacterium abscessus and mycobacterium massiliense.mycobacterium abscessus and mycobacterium massiliense, which cause lung disease, are variable in their clinical manifestation and progression. we hypothesized that mycobacterial genotypes represent their pathogenic phenotypes, which would result in particular genotypes being associated with disease progression.201323511298
expansion of functional human mucosal-associated invariant t cells via reprogramming to pluripotency and redifferentiation.mucosal-associated invariant t (mait) cells play an important physiological role in host pathogen defense and may also be involved in inflammatory disorders and multiple sclerosis. the rarity and inefficient expansion of these cells have hampered detailed analysis and application. here, we report an induced pluripotent stem cell (ipsc)-based reprogramming approach for the expansion of functional mait cells. we found that human mait cells can be reprogrammed into ipscs using a sendai virus harbor ...201323523177
identification and characterization of potential therapeutic candidates in emerging human pathogen mycobacterium abscessus: a novel hierarchical in silico approach.mycobacterium abscessus, a non-tuberculous rapidly growing mycobacterium, is recognized as an emerging human pathogen causing a variety of infections ranging from skin and soft tissue infections to severe pulmonary infections. lack of an optimal treatment regimen and emergence of multi-drug resistance in clinical isolates necessitate the development of better/new drugs against this pathogen. the present study aims at identification and qualitative characterization of promising drug targets in m. ...201323527108
whole-genome sequencing to identify transmission of mycobacterium abscessus between patients with cystic fibrosis: a retrospective cohort study.increasing numbers of individuals with cystic fibrosis are becoming infected with the multidrug-resistant non-tuberculous mycobacterium (ntm) mycobacterium abscessus, which causes progressive lung damage and is extremely challenging to treat. how this organism is acquired is not currently known, but there is growing concern that person-to-person transmission could occur. we aimed to define the mechanisms of acquisition of m abscessus in individuals with cystic fibrosis.201323541540
rare complication after stripping operation: a case report of mycobacterium abscessus infection.mycobacterium abscessus is an acid-fast nontuberculous mycobacterium that grows rapidly in culture. the organism is found in dust, soil, and water and after trauma, it may infect skin and soft tissue. the organism is rarely found in humans, and infections occurring after cardiovascular surgery are rare clinical events. to our knowledge, only a few cases of hemodialysis arteriovenous graft infection and endocarditis caused by m. abscessus have been described. we reported a first case of patient w ...201023555416
the detection and sequencing of a broad-host-range conjugative incp-1β plasmid in an epidemic strain of mycobacterium abscessus subsp. bolletii.an extended outbreak of mycobacterial surgical infections occurred in brazil during 2004-2008. most infections were caused by a single strain of mycobacterium abscessus subsp. bolletii, which was characterized by a specific rpob sequevar and two highly similar pulsed-field gel electrophoresis (pfge) patterns differentiated by the presence of a ∼50 kb band. the nature of this band was investigated.201323565273
epidural abscess caused by mycobacterium abscessus.mycobacterium abscessus is a member of the rapidly growing mycobacterium (rgm). the incidence of mycobacterium abscessus infections has steadily been increasing over the last decade. we report the case of an epidural abscess caused by mycobacterium abscessus. rgm's have infrequently been reported as spinal infections and we found no prior cases reporting m. abscessus as the definitive etiologic agent of an epidural abscess.201223569523
rapid diagnosis of mycobacterium abscessus endophthalmitis.nontuberculous mycobacteria are widely distributed in the environment and have the potential to cause a wide spectrum of infections including pulmonary, bone, soft tissue or ocular infections. they are a rare cause of endophthalmitis, a potentially devastating condition, which may be acquired through contamination of water or antiseptic solutions. diagnosis is often delayed due to low clinical suspicion, resulting in poor clinical outcomes. newer laboratory techniques such as real-time pcr can b ...201323579395
high recurrence rate of lymphadenitis due to nontuberculous mycobacteria and its association with concurrent salmonella infection in taiwan.the objective of this study is to investigate the clinical characteristics of lymphadenitis due to nontuberculous mycobacteria (ntm) in taiwan.201423615164
cord-forming mycobacteria induce dna meshwork formation by human peripheral blood mononuclear cells.mononuclear phagocytes, that is, monocytes and macrophages, are central in the defense against mycobacteria. mycobacterium abscessus is an opportunistic mycobacterial species able to cause chronic pulmonary infections in patients with cystic fibrosis but also soft tissue infections in immunocompetent individuals. pathogenic isolates of m. abscessus with rough colony morphology form cord-like aggregates. in this study, we investigated the in vitro response of human peripheral blood mononuclear ce ...201223620120
nontuberculous mycobacterial infection after fractionated co(2) laser resurfacing.nontuberculous mycobacteria are increasingly associated with cutaneous infections after cosmetic procedures. fractionated co2 resurfacing, a widely used technique for photorejuvenation, has been associated with a more favorable side effect profile than alternative procedures. we describe 2 cases of nontuberculous mycobacterial infection after treatment with a fractionated co2 laser at a private clinic. densely distributed erythematous papules and pustules developed within the treated area within ...201323628077
paediatric lung transplant outcomes vary with mycobacterium abscessus complex species. 201323633613
transition metal-α-amino acid complexes with antibiotic activity against mycobacterium spp.synthetic iridium-, rhodium-, and ruthenium-amino acid complexes with hydrophobic l-amino acids have antibiotic activity against mycobacterium spp., including mycobacterium bovis bcg and the rapidly growing species mycobacterium abscessus and mycobacterium chelonae. concentrations of transition metal-amino acid complexes demonstrating hemolysis or cytotoxicity were 10- to 25-fold higher than were the mics.201323650159
mabsbase: a mycobacterium abscessus genome and annotation database.mycobacterium abscessus is a rapidly growing non-tuberculous mycobacterial species that has been associated with a wide spectrum of human infections. as the classification and biology of this organism is still not well understood, comparative genomic analysis on members of this species may provide further insights on their taxonomy, phylogeny, pathogenicity and other information that may contribute to better management of infections. the mabsbase described in this paper is a user-friendly databa ...201323658631
[new light on the contagiousness of mycobacterium abscessus]. 201323659159
clinical isolates of mycobacterium abscessus in guangzhou area most possibly from the environmental infection showed variable susceptibility.mycobacterium abscessus (m. abscessus) can cause a variety of human infections, involving the lung, skin and soft tissues, and is generally believed to be acquired from environmental sources. the aim of this study was to investigate the molecular diversity and antibiotic susceptibility of m. abscessus isolates as the basis for strategies to improve control and management of infection.201323673103
the first structure of a mycobacteriophage, the mycobacterium abscessus subsp. bolletii phage araucaria.the unique characteristics of the waxy mycobacterial cell wall raise questions about specific structural features of their bacteriophages. no structure of any mycobacteriophage is available, although ∼3,500 have been described to date. to fill this gap, we embarked in a genomic and structural study of a bacteriophage from mycobacterium abscessus subsp. bolletii, a member of the mycobacterium abscessus group. this opportunistic pathogen is responsible for respiratory tract infections in patients ...201323678183
molecular longitudinal tracking of mycobacterium abscessus spp. during chronic infection of the human lung.the mycobacterium abscessus complex is an emerging cause of chronic pulmonary infection in patients with underlying lung disease. the m. abscessus complex is regarded as an environmental pathogen but its molecular adaptation to the human lung during long-term infection is poorly understood. here we carried out a longitudinal molecular epidemiological analysis of 178 m. abscessus spp. isolates obtained from 10 cystic fibrosis (cf) and 2 non cf patients over a 13 year period. multi-locus sequence ...201323696800
cutaneous inoculation of nontuberculous mycobacteria during professional tattooing: a case series and epidemiologic study.the increase in popularity of tattoos has coincided with an increase in reports of cutaneous inoculation of nontuberculous (atypical) mycobacteria (ntm) during the tattooing process. we report 3 ntm infections in otherwise healthy persons who received tattoos, which prompted a multiagency epidemiologic investigation.201323704119
mycobacterium abscessus isolated from municipal water - a potential source of human infection.mycobacterium abscessus is a rapidly growing mycobacterium responsible for progressive pulmonary disease, soft tissue and wound infections. the incidence of disease due to m. abscessus has been increasing in queensland. in a study of brisbane drinking water, m. abscessus was isolated from ten different locations.the aim of this study was to compare genotypically the m. abscessus isolates obtained from water to those obtained from human clinical specimens.201323705674
lung transplant outcomes in cystic fibrosis patients with pre-operative mycobacterium abscessus respiratory infections.mycobacterium abscessus in cystic fibrosis (cf) patients is considered a contraindication to lung transplantation. we examine the post-transplant outcomes of cf patients with m. abscessus pre-transplant.201323710571
epidemiology and outcomes of deep surgical site infections following lung transplantation.we conducted a retrospective study of deep surgical site infections (ssis) among consecutive patients who underwent lung transplantation (ltx) at a single center from 2006 through 2010. thirty-one patients (5%) developed ssis at median 25 days after ltx. empyema was most common (42%), followed by surgical wound infections (29%), mediastinitis (16%), sternal osteomyelitis (6%), and pericarditis (6%). pathogens included gram-positive bacteria (41%), gram-negative bacteria (41%), fungi (10%) and my ...201323710593
Refresh
firstprev
12345678910...
nextlast
Displaying items 401 - 500 of 1047