| [Diagnosis and treatment of atypical mycobacterial infections of the respiratory tract]. | INTRODUCTION: Non tuberculous mycobacteria (NTM), unlike tuberculous mycobacteria, are not strictly human pathogens. The diagnosis of infection and the choice of treatment remain difficult. BACKGROUND: Evidence of a NTM in a pulmonary sample is not synonymous with infection. The diagnosis depends on the association of clinical, radiological and microbiological factors. If a NTM is isolated from a respiratory sample, the probability of infection depends on the species. The main NTMs responsible f ... | 2011 | 22152937 |
| Antibacterial activities of dendritic amphiphiles against nontuberculous mycobacteria. | The anti-mycobacterial activities of nine series of dicarboxyl and tricarboxyl dendritic amphiphiles with one alkyl, two alkyl, and cholestanyl tails against Mycobacterium abscessus, Mycobacterium avium, Mycobacterium chelonae, Mycobacterium marinum and Mycobacterium smegmatis have been measured. The dendritic amphiphiles overcame the limited aqueous solubility of natural long-chain fatty acids, alcohols, and amines to enable profiling the susceptibilities of the different mycobacterial species ... | 2011 | 22209468 |
| multifocal infection of mycobacterium other than tuberculosis mimicking a soft tissue tumor of the extremity. | mycobacterium other than tuberculosis infections rarely develop in healthy individuals, but direct inoculation such as contaminated acupuncture can cause mycobacteriosis even in an immunocompetent host. a 56-year-old woman gradually developed pain on the anterior aspect of the left knee and the distal thigh after hiking without trauma. she received acupuncture for 3 consecutive days on the bilateral knees at the suprapatellar and infrapopliteal areas. after acupuncture, mild localized heat and p ... | 2011 | 22146217 |
| conditional gene expression in mycobacterium abscessus. | mycobacterium abscessus is an emerging human pathogen responsible for lung infections, skin and soft-tissue infections and disseminated infections in immunocompromised patients. it may exist either as a smooth (s) or rough (r) morphotype, the latter being associated with increased pathogenicity in various models. genetic tools for homologous recombination and conditional gene expression are desperately needed to allow the study of m. abscessus virulence. however, descriptions of knock-out (ko) m ... | 2011 | 22195042 |
| respiratory outbreak of mycobacterium abscessus subspecies massiliense in a lung transplant and cystic fibrosis center. | | 2012 | 22246710 |
| mycobacterium abscessus glycopeptidolipid prevents respiratory epithelial tlr2 signaling as measured by hβd2 gene expression and il-8 release. | mycobacterium abscessus has emerged as an important cause of lung infection, particularly in patients with bronchiectasis. innate immune responses must be highly effective at preventing infection with m. abscessus because it is a ubiquitous environmental saprophyte and normal hosts are not commonly infected. m. abscessus exists as either a glycopeptidolipid (gpl) expressing variant (smooth phenotype) in which gpl masks underlying bioactive cell wall lipids, or as a variant lacking gpl which is i ... | 2011 | 22216191 |
| molecular identification and typing of mycobacterium massiliense isolated from postsurgical infections in brazil. | one hundred thirty-one cases of postsurgical infections were reported in southern region of brazil between august 2007 and january 2008. thirty-nine (29.8%) cases were studied; this report describes epidemiological findings, species identification, antimicrobial susceptibility and clonal diversity of rapidly growing mycobacteria isolated in this outbreak. | 2011 | 22230849 |
| cord-forming mycobacteria induce dna meshwork formation by human peripheral blood mononuclear cells. | mononuclear phagocytes, that is, monocytes and macrophages, are central in the defense against mycobacteria. mycobacterium abscessus is an opportunistic mycobacterial species able to cause chronic pulmonary infections in patients with cystic fibrosis but also soft tissue infections in immunocompetent individuals. pathogenic isolates of m. abscessus with rough colony morphology form cord-like aggregates. in this study, we investigated the in vitro response of human peripheral blood mononuclear ce ... | 2012 | 23620120 |
| mycobacteriosis in zebrafish colonies. | mycobacteriosis, a chronic bacterial infection, has been associated with severe losses in some zebrafish facilities and low-level mortalities and unknown impacts in others. the occurrence of at least six different described species (mycobacterium abscessus, m. chelonae, m. fortuitum, m. haemophilum, m. marinum, m. peregrinum) from zebrafish complicates diagnosis and control because each species is unique. as a generalization, mycobacteria are often considered opportunists, but m. haemophilum and ... | 2012 | 23382341 |
| rapid tests for the detection of the mycobacterium abscessus subsp. bolletii strain responsible for an epidemic of surgical-site infections in brazil. | a single strain of mycobacterium abscessus subsp. bolletii, characterised by a particular rpob sequevar and two highly related pulsed field gel electrophoresis patterns has been responsible for a nationwide outbreak of surgical infections in brazil since 2004. in this study, we developed molecular tests based on polymerase chain reaction restriction-enzyme analysis (pra) and sequencing for the rapid identification of this strain. sequences of 15 dna regions conserved in mycobacteria were retriev ... | 2012 | 23295745 |
| mycobacterium abscessus pneumonia in an atlantic bottlenose dolphin (tursiops truncatus). | mycobacterium abscessus pneumonia was diagnosed antemortem in a 23-yr-old male atlantic bottlenose dolphin (tursiops truncatus). clinical signs included lethargy, hyporexia, coughing, and bloody respiratory discharge. diagnostic findings included neutrophilic leukocytosis, anemia, elevated erythrocyte sedimentation rate, and repeated forceful exhaled breath (sputum) cytology, with acute inflammatory cells and acid-fast positive beaded rods. the bacteria were initially identified free in the sput ... | 2012 | 23272373 |
| nontuberculous mycobacterial disease is not a contraindication to lung transplantation in patients with cystic fibrosis: a retrospective analysis in a danish patient population. | whether nontuberculous mycobacterial (ntm) disease is a contraindication to lung transplantation remains controversial. we conducted a nationwide study to evaluate the clinical importance of ntm infection among lung transplant patients with cystic fibrosis (cf) in denmark and to determine if ntm infection poses a contraindication to lung transplantation. all cf patients with current or prior ntm who had undergone lung transplantation were identified. out of 52 lung transplant patients with cf 9 ... | 2012 | 23267788 |
| successful treatment with chemotherapy and corticosteroids of pulmonary mycobacterium abscessus infection accompanied by pleural effusion. | we describe a 50-year-old woman with rapidly progressive pulmonary mycobacterium abscessus (m. abscessus) infection accompanied by pleural effusion and organizing pneumonia (op). ct scan showed consolidation, centrilobular shadows, ground-glass opacity (ggo), and cavities. a transbronchial lung biopsy showed nonnecrotizing granuloma surrounded by infiltrative lymphocyte-dominant inflammatory cells, and lymphocytes in bronchoalveolar lavage fluid (balf) were increased. we considered op occurred s ... | 2012 | 23263189 |
| environmental effects on protection against mycobacterium tuberculosis after immunization with ad85a. | previously we have shown that intradermal (i.d.) immunization with a recombinant adenovirus expressing antigen 85a (ad85a) induced a strong splenic cd8t cell response in balb/c mice but a weak lung immune response and did not protect mice against challenge with mycobacterium tuberculosis (mtb). after moving to a new animal house, the same i.d. immunization induced a strong lung immune response and the mice were protected against mtb challenge. increased numbers of antigen 85a-specific cd8 cells ... | 2012 | 23262169 |
| heterologous expression of mycobacterial esx complexes in escherichia coli for structural studies is facilitated by the use of maltose binding protein fusions. | the expression of heteroligomeric protein complexes for structural studies often requires a special coexpression strategy. the reason is that the solubility and proper folding of each subunit of the complex requires physical association with other subunits of the complex. the genomes of pathogenic mycobacteria encode many small protein complexes, implicated in bacterial fitness and pathogenicity, whose characterization may be further complicated by insolubility upon expression in escherichia col ... | 2013 | 24312350 |
| in vitro activity of tp-271 against mycobacterium abscessus, mycobacterium fortuitum, and nocardia species. | the in vitro activities of tp-271, a novel fluorocycline antimicrobial, against 22 isolates of mycobacterium abscessus, 22 isolates of mycobacterium fortuitum, and 19 isolates of nocardia spp. were studied by a microtiter broth dilution method. the mic(90)s for m. abscessus, m. fortuitum, and nocardia spp. were 0.5 μg/ml, 0.03 μg/ml, and 8 μg/ml, respectively. tp-271 was significantly more active than the respective control drug in virtually all tests. | 2012 | 22585214 |
| the detection and sequencing of a broad-host-range conjugative incp-1β plasmid in an epidemic strain of mycobacterium abscessus subsp. bolletii. | an extended outbreak of mycobacterial surgical infections occurred in brazil during 2004-2008. most infections were caused by a single strain of mycobacterium abscessus subsp. bolletii, which was characterized by a specific rpob sequevar and two highly similar pulsed-field gel electrophoresis (pfge) patterns differentiated by the presence of a ∼50 kb band. the nature of this band was investigated. | 2013 | 23565273 |
| the significance of mycobacterium abscessus subspecies abscessus isolation during mycobacterium avium complex lung disease therapy. | isolation of mycobacterium abscessus subspecies abscessus (maa) is common during mycobacterium avium complex (mac) lung disease therapy, but there is limited information about the clinical significance of the maa isolates. | 2014 | 25357074 |
| genome analysis reveals three genomospecies in mycobacterium abscessus. | mycobacterium abscessus complex, the third most frequent mycobacterial complex responsible for community- and health care-associated infections in developed countries, comprises of m. abscessus subsp. abscessus and m. abscessus subsp. bolletii reviously referred as mycobacterium bolletii and mycobacterium massiliense. the diversity of this group of opportunistic pathogens is poorly described. | 2014 | 24886480 |
| vaccine strategies against cystic fibrosis pathogens. | a great number of cystic fibrosis (cf) pathogens such as pseudomonas aeruginosa, the burkholderia cepacia and the mycobacterium abscessus complex raised difficult therapeutic problems due to their intrinsic multi-resistance to numerous antibiotics. vaccine strategies represent one of the key weapons against these multi-resistant bacteria in a number of clinical settings like cf. different strategies are considered in order to develop such vaccines, linked either to priming the host response, or ... | 2015 | 26618824 |
| cysteamine as a future intervention in cystic fibrosis against current and emerging pathogens: a patient-based ex vivo study confirming its antimicrobial and mucoactive potential in sputum. | cysteamine has recently been shown to have in vitro properties potentially therapeutically beneficial in cystic fibrosis (cf). in this study we investigated the antimicrobial and mucolytic activity of cysteamine against the complex biologic matrix of cf sputum. | 2015 | 26629546 |
| mycobacterium abscessus complex identification with matrix-assisted laser desorption ionization-time of flight mass spectrometry. | we determined that the vitek ms plus matrix-assisted laser desorption ionization-time of flight mass spectrometry using research-use-only (ruo) v.4.12 and in vitro-diagnostic (ivd) v.3.0 databases accurately identified 41 mycobacterium abscessus subsp. abscessus and 13 m. abscessus subsp. massiliense isolates identified by whole-genome sequencing to the species but not the subspecies level, from middlebrook 7h11 and burkholderia cepacia selective agars. peak analysis revealed three peaks potenti ... | 2015 | 25948607 |
| "you can't always get what you want…" the mycobacterium abscessus issue. name versus function. | | 2015 | 25786149 |
| epidemiology of nontuberculous mycobacteria among patients with cystic fibrosis in scandinavia. | nontuberculous mycobacteria (ntm) are an emerging threat to cystic fibrosis (cf) patients but their epidemiology is not well described. | 2015 | 25178871 |
| nontuberculous mycobacteria in cystic fibrosis. | nontuberculous mycobacteria (ntm) are found in approximately 10 % of cystic fibrosis (cf) patients, but only a portion will develop ntm disease. the management of cf lung disease should be optimized, including antibiotic therapy targeted to the individual's usual airway bacteria, prior to considering treatment for ntm lung disease. those who meet criteria for ntm lung disease may not necessarily require treatment and could be monitored expectantly if symptoms and radiographic findings are minima ... | 2016 | 28035194 |
| functional characterization of the mycobacterium abscessus genome coupled with condition specific transcriptomics reveals conserved molecular strategies for host adaptation and persistence. | mycobacterium abscessus subsp. abscessus (mab) is a highly drug resistant mycobacterium and the most common respiratory pathogen among the rapidly growing non-tuberculous mycobacteria. mab is also one of the most deadly of the emerging cystic fibrosis (cf) pathogens requiring prolonged treatment with multiple antibiotics. in addition to its "mycobacterial" virulence genes, the genome of mab harbours a large accessory genome, presumably acquired via lateral gene transfer including homologs shared ... | 2016 | 27495169 |
| comparing the harmful effects of nontuberculous mycobacteria and gram negative bacteria on lung function in patients with cystic fibrosis. | to better understand the relative effects of infection with nontuberculous mycobacteria and gram negative bacteria on lung function decline in cystic fibrosis, we assessed the impact of each infection in a danish setting. | 2016 | 26482717 |
| assessment of the microbial constituents of the home environment of individuals with cystic fibrosis (cf) and their association with lower airways infections. | cystic fibrosis (cf) airways are colonized by a polymicrobial community of organisms, termed the cf microbiota. we sought to define the microbial constituents of the home environment of individuals with cf and determine if it may serve as a latent reservoir for infection. | 2016 | 26859493 |
| mycobacterium abscessus phospholipase c expression is induced during coculture within amoebae and enhances m. abscessus virulence in mice. | mycobacterium abscessus is a pathogenic, rapidly growing mycobacterium involved in pulmonary and cutaneo-mucous infections worldwide, to which cystic fibrosis patients are exquisitely susceptible. the analysis of the genome sequence of m. abscessus showed that this bacterium is endowed with the metabolic pathways typically found in environmental microorganisms that come into contact with soil, plants, and aquatic environments, where free-living amoebae are frequently present. m. abscessus also c ... | 2014 | 25486995 |
| the antibiotic resistance arrow of time: efflux pump induction is a general first step in the evolution of mycobacterial drug resistance. | we hypothesize that low-level efflux pump expression is the first step in the development of high-level drug resistance in mycobacteria. we performed 28-day azithromycin dose-effect and dose-scheduling studies in our hollow-fiber model of disseminated mycobacterium avium-m. intracellulare complex. both microbial kill and resistance emergence were most closely linked to the within-macrophage area under the concentration-time curve (auc)/mic ratio. quantitative pcr revealed that subtherapeutic azi ... | 2012 | 22751536 |
| gallium compounds exhibit potential as new therapeutic agents against mycobacterium abscessus. | the rapidly growing nontuberculous mycobacterial species mycobacterium abscessus has recently emerged as an important pathogen in patients with cystic fibrosis (cf). treatment options are limited because of the organism's innate resistance to standard antituberculous antibiotics, as well as other currently available antibiotics. new antibiotic approaches to the treatment of m. abscessus are urgently needed. the goal of the present study was to assess the growth-inhibitory activity of different g ... | 2015 | 26033732 |
| the first structure of a mycobacteriophage, the mycobacterium abscessus subsp. bolletii phage araucaria. | the unique characteristics of the waxy mycobacterial cell wall raise questions about specific structural features of their bacteriophages. no structure of any mycobacteriophage is available, although ∼3,500 have been described to date. to fill this gap, we embarked in a genomic and structural study of a bacteriophage from mycobacterium abscessus subsp. bolletii, a member of the mycobacterium abscessus group. this opportunistic pathogen is responsible for respiratory tract infections in patients ... | 2013 | 23678183 |
| essential engagement of toll-like receptor 2 in initiation of early protective th1 response against rough variants of mycobacterium abscessus. | although mycobacterium abscessus (m. abscessus) is becoming more prevalent in patients without overt immunodeficiency, little is known about the factors that contribute to disease susceptibility. this study was undertaken to investigate how toll-like receptor 2 (tlr2) functionally contributes to the generation of protective immunity against m. abscessus in a morphotype-specific manner. we found that tlr2-/- mice were extremely susceptible to an intravenous (i.v.) model of infection by m. abscess ... | 2015 | 25644006 |
| mycocap - mycobacterium comparative analysis platform. | mycobacterium spp. are renowned for being the causative agent of diseases like leprosy, buruli ulcer and tuberculosis in human beings. with more and more mycobacterial genomes being sequenced, any knowledge generated from comparative genomic analysis would provide better insights into the biology, evolution, phylogeny and pathogenicity of this genus, thus helping in better management of diseases caused by mycobacterium spp.with this motivation, we constructed mycocap, a new comparative analysis ... | 2015 | 26666970 |
| inhaled amikacin for treatment of refractory pulmonary nontuberculous mycobacterial disease. | treatment of pulmonary nontuberculous mycobacteria, especially mycobacterium abscessus, requires prolonged, multidrug regimens with high toxicity and suboptimal efficacy. options for refractory disease are limited. | 2014 | 24460437 |
| emerging bacterial pathogens and changing concepts of bacterial pathogenesis in cystic fibrosis. | chronic suppurative lower airway infection is a hallmark feature of cystic fibrosis (cf). decades of experience in clinical microbiology have enabled the development of improved technologies and approaches for the cultivation and identification of microorganisms from sputum. it is increasingly apparent that the microbial constituents of the lower airways in cf exist in a dynamic state. indeed, while changes in prevalence of various pathogens occur through ageing, differences exist in successive ... | 2015 | 25881770 |
| reduced risk of nontuberculous mycobacteria in cystic fibrosis adults receiving long-term azithromycin. | azithromycin reduces exacerbations in cystic fibrosis (cf) patients. our aim was to investigate its association with nontuberculous mycobacteria isolation and macrolide susceptibility. | 2015 | 25735458 |
| real-time monitoring of hydrogen cyanide (hcn) and ammonia (nh₃) emitted by pseudomonas aeruginosa. | we present the real-time monitoring of hydrogen cyanide (hcn) production from pseudomonas aeruginosa (p. aeruginosa) strains in vitro, using laser-based photoacoustic spectroscopy. simultaneously, the production of ammonia (nh3) was measured, and the influence of different factors (e.g. the medium, temperature and antibiotics treatment) was assessed. both reference strains and clinical isolates of patients with cf were studied, and compared to other pathogens commonly present in lungs/airways of ... | 2015 | 25634638 |
| antibiotic management of lung infections in cystic fibrosis. ii. nontuberculous mycobacteria, anaerobic bacteria, and fungi. | airway infections are a key component of cystic fibrosis (cf) lung disease. whereas the approach to common pathogens such as pseudomonas aeruginosa is guided by a significant body of evidence, other infections often pose a considerable challenge to treating physicians. in part i of this series on the antibiotic management of difficult lung infections, we discussed bacterial organisms including methicillin-resistant staphylococcus aureus, gram-negative bacterial infections, and treatment of multi ... | 2014 | 25167882 |
| risk factors for mycobacterium abscessus infection in cystic fibrosis patients; a case-control study. | mycobacterium abscessus is a nontuberculous mycobacterium that is increasingly recognized as an opportunistic pathogen in cystic fibrosis (cf) patients. factors that predispose cf patients to infection by this environmental bacterium remain unknown. in a case-control study of 22 cf patients with m. abscessus infection and 22 cf controls, we investigated risk factors for mab infection as well as the positioning of mab infection in relation to the other pathogens infecting cf patients. no clear ri ... | 2012 | 22342403 |
| structure elucidation and biological activity of antibacterial compound from micromonospora auratinigra, a soil actinomycetes. | the aim of this study was to isolate and characterize the bioactive compound of micromonospora auratinigra, hk-10 and its antibacterial inhibitory mechanism. | 2016 | 27406903 |
| biofilm colonization in chronic treatment refractory infections presenting with discharging sinuses: a study in a tertiary care hospital of eastern india. | treatment refractory chronic recurrent infections mean those chronic infections which recur by same causal agents with similar drug responsiveness after apparent relief following full course of recommended antimicrobial management. | 2017 | 28367029 |
| contamination of hypertonic saline solutions in use by cystic fibrosis patients in israel. | treatment of cystic fibrosis (cf) patients with inhaled hypertonic saline (hs) solutions is safe, beneficial and reduces exacerbation rates. we studied contamination of solutions used by israeli cf patients for prolonged periods. | 2014 | 24484849 |
| respiratory infections in patients with cystic fibrosis undergoing lung transplantation. | cystic fibrosis is an inherited disease characterised by chronic respiratory infections associated with bronchiectasis. lung transplantation has helped to extend the lives of patients with cystic fibrosis who have advanced lung disease. however, persistent, recurrent, and newly acquired infections can be problematic. classic cystic fibrosis-associated organisms, such as staphylococcus aureus and pseudomonas aeruginosa, are generally manageable post-transplantation, and are associated with favour ... | 2014 | 24461904 |
| survival after lung transplantation for cystic fibrosis in sweden†. | in sweden, lung transplantation has been performed in patients with end-stage lung disease since 1990. we assessed survival after lung transplantation for cystic fibrosis (cf) with focus on early mortality and outcome for patients infected with certain multiresistant bacteria, considered a relative contraindication for lung transplantation. | 2017 | 28364441 |
| vaccine strategies against bacterial pathogens in cystic fibrosis patients. | a large number of cystic fibrosis pathogens such as bacteria of the burkholderia cepacia complex, pseudomonas aeruginosa, or mycobacterium abscessus are associated with complex therapeutic problems due to their inherent resistance to antibiotics. no vaccine is currently available against those pathogens. vaccines are therefore crucial to combat these multidrug-resistant bacteria in specific clinical situations including cystic fibrosis. various strategies may be considered to develop these vacci ... | 2016 | 26746326 |
| a novel culture medium for isolation of rapidly-growing mycobacteria from the sputum of patients with cystic fibrosis. | isolation of mycobacteria from the sputum of patients with cystic fibrosis (cf) is challenging due to the overgrowth of cultures by other bacteria and fungi. in this setting, burkholderia cepacia selective agar (bcsa) has been recommended as a convenient and effective culture medium for the isolation of rapidly-growing, non-tuberculous mycobacteria (ntm). a novel selective culture medium (rgm medium) was evaluated for the isolation of rapidly-growing ntm from the sputum of children and adults wi ... | 2016 | 26002312 |
| epidemiology of infection and current guidelines for infection prevention in cystic fibrosis patients. | the spectrum of bacterial pathogens encountered in cystic fibrosis (cf) lung disease has expanded over the last decade. in addition to established pathogens, such as pseudomonas aeruginosa, burkholderia cepacia complex and staphylococcus aureus, novel gram-negative non-fermenter bacteria and non-tuberculous mycobacteria have gained in clinical significance. air sampling performed in inpatient and outpatient clinics, and analysis of cough aerosols expelled by cf patients provides evidence for pot ... | 2015 | 25791927 |
| lung transplantation in patients with cystic fibrosis: special focus to infection and comorbidities. | despite advances in medical care, patients with cystic fibrosis still face limited life expectancy. the most common cause of death remains respiratory failure. end-stage cystic fibrosis can be treated with lung transplantation and is the third most common reason for which the procedure is performed. outcomes for cystic fibrosis are better than most other lung diseases, but remain limited (5-year survival 60%). for patients with advanced disease lung transplantation appears to improve survival. o ... | 2014 | 24655065 |
| an evaluation of different steam disinfection protocols for cystic fibrosis nebulizers. | contamination is a key element in cystic fibrosis. for this reason, nebulizer hygiene is an important, but complex and time-consuming task for cystic fibrosis patients. the aim of this study was to compare different steam disinfection and drying protocols. | 2016 | 26233897 |
| evaluation of the antimicrobial activity of the mastoparan polybia-mpii isolated from venom of the social wasp pseudopolybia vespiceps testacea (vespidae, hymenoptera). | mastoparans, a class of peptides found in wasp venom, have significant effects following a sting as well as useful applications in clinical practice. among these is their potential use in the control of micro-organisms that cause infectious diseases with a significant impact on society. thus, the present study describes the isolation and identification of a mastoparan peptide from the venom of the social wasp pseudopolybia vespiceps and evaluated its antimicrobial profile against bacteria (staph ... | 2017 | 28108242 |
| mycobacterium abscessus infection in a burn intensive care unit patient. | infection is the leading cause of death in burn patients. historically, this was due to burn wound sepsis but pneumonia has now emerged as the most common source. in light of the increasing incidence of multi-drug-resistant organisms, the description of rare infections is paramount in continuing the fight against deadly pathogens. we aim to describe the second case of non-tuberculous mycobacterium (ntm) reported in a burn patient. difficulties in diagnosis and management will also be highlighted ... | 2014 | 25493353 |
| advantages of using matrix-assisted laser desorption ionization-time of flight mass spectrometry as a rapid diagnostic tool for identification of yeasts and mycobacteria in the clinical microbiological laboratory. | yeast and mycobacteria can cause infections in immunocompromised patients and normal hosts. the rapid identification of these organisms can significantly improve patient care. there has been an increasing number of studies on using matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) for rapid yeast and mycobacterial identifications. however, studies on direct comparisons between the bruker biotyper and biomérieux vitek ms systems for the identification of ... | 2013 | 24048537 |
| non-tuberculous mycobacteria and their surface lipids efficiently induced il-17 production in human t cells. | interleukin-17 (il-17) is produced by a subset of cd4(+) t helper (th) lymphocytes known as th17 cells. in humans, il-1β, enhanced by il-6 and il-23 is crucial for differentiation of these cells. il-17 evokes inflammation and is involved in host defence against microorganisms, although little is known about its role in diseases caused by non-tuberculous mycobacteria. the genus mycobacterium contains both obligate and opportunistic pathogens as well as saprophytes, and the mycobacterial cell enve ... | 2012 | 22939918 |
| the surface lipids of non-tuberculous mycobacteria suppress production of phagocyte activating cytokines in human peripheral blood mononuclear cells. | the genus mycobacterium includes obligate pathogens as well as opportunistic and non-pathogenic species ubiquitous in the environment. mycobacteria have a unique cell wall abundant in lipids. here we investigated cytokine production by human peripheral blood mononuclear cells (pbmc) in response to the opportunistic mycobacteria mycobacterium avium and mycobacterium abscessus, the non-pathogenic mycobacterium gordonae and extracted surface lipids from the three species. the cytokine response elic ... | 2012 | 22497872 |
| inhibition of β-lactamases of mycobacteria by avibactam and clavulanate. | mycobacterium tuberculosis and mycobacterium abscessus produce broad-spectrum class a β-lactamases, blac and bla mab , which are inhibited by clavulanate and avibactam, respectively. blac differs from bla mab at ambler position 132 in the conserved motif sdn (sdg versus sdn, respectively). here, we investigated whether this polymorphism could account for the inhibition specificity of β-lactamases from slowly and rapidly growing mycobacteria. | 2017 | 28039278 |
| intrinsic rifamycin resistance of mycobacterium abscessus is mediated by adp-ribosyltransferase mab_0591. | rifampicin, a potent first-line tb drug of the rifamycin group, shows only little activity against the emerging pathogen mycobacterium abscessus. reportedly, bacterial resistance to rifampicin is associated with polymorphisms in the target gene rpob or the presence of enzymes that modify and thereby inactivate rifampicin. the aim of this study was to investigate the role of the mab_0591 (arrmab)-encoded rifampicin adp-ribosyltransferase (arr_mab) in innate high-level rifampicin resistance in m. ... | 2017 | 27999011 |
| complete genome sequencing and comparative analyses of broad-spectrum antimicrobial-producing micromonospora sp. hk10. | micromonospora genus produces >700 bioactive compounds of medical relevance. in spite of its ability to produce high number of bioactive compounds, no genome sequence is available with comprehensive secondary metabolite gene clusters analysis for anti-microbial producing micromonospora strains. thus, here we contribute the full genome sequence of micromonospora sp. hk10 strain, which has high antibacterial activity against several important human pathogens like, mycobacterium abscessus, mycobact ... | 2016 | 27609432 |
| lack of antimicrobial bactericidal activity in mycobacterium abscessus. | antibiotic therapy of infections caused by the emerging pathogen mycobacterium abscessus is challenging due to the organism's natural resistance toward most clinically available antimicrobials. we investigated the bactericidal activity of antibiotics commonly administered in m. abscessus infections in order to better understand the poor therapeutic outcome. time-kill curves were generated for clinical m. abscessus isolates, mycobacterium smegmatis, and escherichia coli by using antibiotics commo ... | 2014 | 24752273 |
| characterization of broad-spectrum mycobacterium abscessus class a β-lactamase. | imipenem and cefoxitin are used to treat mycobacterium abscessus infections and have moderate activity against this fast-growing mycobacterium (mic₅₀ of 16 and 32 mg/l, respectively). m. abscessus is highly resistant to most other β-lactams, although the underlying mechanisms have not been explored. here, we characterized m. abscessus class a β-lactamase (bla(mab)) and investigated its role in β-lactam resistance. | 2014 | 24132992 |
| selected insights from application of whole-genome sequencing for outbreak investigations. | the advent of high-throughput whole-genome sequencing has the potential to revolutionize the conduct of outbreak investigation. because of its ultimate resolution power for differentiating between closely related pathogen strains, whole-genome sequencing could augment the traditional epidemiologic investigations of infectious disease outbreaks. | 2013 | 23856896 |
| patients with inhibitory and neutralizing auto-antibodies to interferon-γ resemble the sporadic adult-onset phenotype of mendelian susceptibility to mycobacterial disease (msmd) lacking bacille calmette-guerin (bcg)-induced diseases. | to recognize patients with inhibitory and neutralizing auto-antibodies to interferon-γ (autoabs-ifn-γ) presenting with the sporadic phenotype of mendelian susceptibility to mycobacterial disease (msmd) mainly characterized by recurrent intracellular mycobacterium or/and salmonella infections, we comprehensively investigated il12/23-ifn-γ signaling, candidate genetic sequencings or/and protein expressions of il12rb1, ifnrg1, il12p40, ifnrg2, stat1, ikka, nemo, cybb and irf8 in four patients. thei ... | 2013 | 23083630 |
| b-cell lymphoma in a patient with complete interferon gamma receptor 1 deficiency. | immunosuppression-associated lymphoproliferative disorders can be related to primary as well as acquired immune disorders. interferon gamma receptor (ifn-γr) deficiency is a rare primary immune disorder, characterized by increased susceptibility to mycobacterial infections. here we report the first case of an epstein barr virus (ebv) related b-cell lymphoma in a patient with complete ifn-γr1 deficiency. the patient was a 20-year-old man with homozygous 22cdel in ifngr1 resulting in complete abse ... | 2013 | 23800860 |
| nontuberculous mycobacterial pulmonary infections. | pulmonary infections due to nontuberculous mycobacteria (ntm) are increasingly recognized worldwide. although over 150 different species of ntm have been described, pulmonary infections are most commonly due to mycobacterium avium complex (mac), mycobacterium kansasii, and mycobacterium abscessus. the identification of these organisms in pulmonary specimens does not always equate with active infection; supportive radiographic and clinical findings are needed to establish the diagnosis. it is dif ... | 2014 | 24624285 |
| a patient-based analysis of the geographic distribution of mycobacterium avium complex, mycobacterium abscessus, and mycobacterium kansasii infections in the united states. | | 2017 | 28390637 |
| post-lower segment cesarean section wound infection with mycobacterium abscessus. | | 2017 | 28366911 |
| a clofazimine-containing regimen for the treatment of mycobacterium abscessus lung disease. | patients with lung disease caused by mycobacterium abscessus subspecies abscessus (hereafter m. abscessus) typically have poor treatment outcomes. although clofazimine (cfz) has been increasingly used in the treatment of m. abscessus lung disease in clinical practice, there are no published data on its effectiveness for this disease. this study sought to evaluate the clinical efficacy of a cfz-containing regimen for the treatment of m. abscessus lung disease. we performed a retrospective review ... | 2017 | 28348153 |
| atypical mycobacterial infections of the upper extremity: becoming more atypical? | background: atypical mycobacterial infections are uncommon and characterized by a chronic, indolent course before diagnosis. historically, these infections are associated with marine-related injury and/or immune dysfunction. our institution has successfully diagnosed and treated an increasing number of these infections. the objective of this study is to review our experience with these rare infections and verify the developing trends encountered. methods: a retrospective review was performed for ... | 2017 | 28344532 |
| pathogen identification in suspected cases of pyogenic spondylodiscitis. | pyogenic spinal infection continues to represent a worldwide problem. in approximately one-third of patients with pyogenic spondylodiscitis, the infectious agent is never identified. of the cases that lead to organismal identification, bacteria are more commonly isolated from the spine rather than fungi and parasites. this study applied universal prokaryotic 16s rrna pcr as a rapid diagnostic tool for the detection of bacterial agents in specimens from patients suspected of pyogenic spondylodisc ... | 2017 | 28337426 |
| evaluation of the new genotype ntm-dr kit for the molecular detection of antimicrobial resistance in non-tuberculous mycobacteria. | non-tuberculous mycobacteria (ntm) are emerging pathogens causing difficult-to-treat infections. we tested a new assay (genotype ntm-dr) that detects natural and acquired resistance mechanisms to macrolides and aminoglycosides in frequently isolated ntm species. | 2017 | 28333340 |
| combinations of avibactam and carbapenems exhibit enhanced potencies against drug-resistant mycobacterium abscessus. | the objective of this study was to assess if avibactam, a new β-lactamase inhibitor, can restore the potency of carbapenems, a sub-class of β-lactams, against mycobacterium abscessus clinical isolates. | 2017 | 28326811 |
| isolation and characterization of nontuberculous mycobacteria from patients with pulmonary tuberculosis in ghana. | nontuberculous mycobacterial (ntm) species are assuming public health importance in pulmonary diseases; they are increasingly being isolated, and importantly, most ntms do not respond to routine tuberculosis (tb) drugs. this study aimed to identify ntms isolated from pulmonary tb cases and also determine their susceptibility to streptomycin (str), isoniazid (inh), and rifampicin (rif). | 2017 | 28317808 |
| port-site infections by nontuberculous mycobacterium: a retrospective clinico-microbiological study. | port-site infection (psi) is a prevailing, chronic, nagging, treatment refractory complication of laparoscopic surgery (ls). it neutralizes the advantages of minimally invasive surgery and increases morbidity, treatment cost of patient, leading to loss of confidence on operating surgeon. psis are preventable with appropriate preoperative, intraoperative, and postoperative measures. atypical mycobacterium is most commonly associated with nonhealing postlaparoscopic wound infections, causing outbr ... | 2017 | 28317802 |
| mycobacterium abscessus subsp. abscessus is capable of degrading pseudomonas aeruginosa quinolone signals. | pseudomonas aeruginosa employs 2-heptyl-3-hydroxy-4(1h)-quinolone (the pseudomonas quinolone signal, pqs) and 2-heptyl-4(1h)-quinolone (hhq) as quorum sensing signal molecules, which contribute to a sophisticated regulatory network controlling the production of virulence factors and antimicrobials. we demonstrate that mycobacterium abscessus(t) and clinical m. abscessus isolates are capable of degrading these alkylquinolone signals. genome sequences of 50 clinical m. abscessus isolates indicated ... | 2017 | 28303132 |
| comparative genome analyses of mycobacteria give better insights into their evolution. | mycobacteria a genus of actinobacteria are widespread in nature ranging from soil-dwelling saprophytes to human and animal pathogens. the rate of growth has been a classifying factor for the mycobacterium spp., dividing them into the rapid growers and the slow growers. here we have performed a comparative genome study of mycobacterial species in order to get better understanding of their evolution, particularly to understand the distinction between the rapid and slow growers. our study shows tha ... | 2017 | 28291784 |
| granulomatous lobular mastitis associated with mycobacterium abscessus in south china: a case report and review of the literature. | mycobacteria, which are known as rapidly growing bacteria, are pathogens that are responsible for cutaneous or subcutaneous infections that especially occur after injection, trauma, or surgery. in this report, we describe a species of mycobacterium abscessus that was isolated from a breast abscess in a patient who was previously diagnosed with granulomatous lobular mastitis (glm). this current case is the first ever presented case of glm associated with m. abscessus documented in south china. th ... | 2017 | 28286681 |
| non-disulfide-bridge peptide 5.5 from the scorpion hadrurus gertschi inhibits the growth of mycobacterium abscessus subsp. massiliense. | multi-drug resistant microorganisms have been a growing concern during the last decades due to their contribution in mortality rates worldwide. antimicrobial peptides (amps) are broad spectrum antimicrobial agents that display potent microbicidal activity against a wide range of microorganisms. amps generally have a rapid mode of action that reduces the risk of resistance developing among pathogens. in this study, an amp derived from scorpion venom, ndbp-5.5, was evaluated against mycobacterium ... | 2017 | 28275372 |
| expect the unexpected: mycobacterial infection in post total knee arthroplasty patients. | orthopaedic surgeons rarely encounter mycobacterial infections in post total knee arthroplasty (tka) patients. we present series of two cases to create awareness among clinicians to expect the unexpected. tuberculosis typical/ atypical is a hidden culprit in catch clinical situations when chronic infection is suspected, but the lab investigations are negative in persistently symptomatic patients. in such situations clinicians should suspect atypical or complex mycobacterial infections and evalua ... | 2017 | 28274011 |
| antimicrobial susceptibility testing of rapidly growing mycobacteria isolated in japan. | difficult-to-treat infections caused by rapidly growing mycobacteria (rgm) are increasingly observed in clinical settings. however, studies on antimicrobial susceptibilities and effective treatments against rgm in japan are limited. | 2017 | 28270102 |
| in vitro activity of bedaquiline against nontuberculous mycobacteria in china. | the main goal of our study was to evaluate in vitro drug susceptibility of bedaquiline against six prevalent pathogenic nontuberculous mycobacteria (ntm) diseases in china. in addition, we investigated the potential molecular mechanism contributing to the bedaquiline resistance in these different ntm species. for slowly growing mycobacteria (sgm), bedaquiline exhibited the highest activity against mycobacterium avium, the mic50 and mic90 were 0.03 and 16 mg/l, respectively. of rapidly growing my ... | 2017 | 28242674 |
| mycobacterial arthritis and synovitis in painted reed frogs (hyperolius marmoratus). | several species of atypical mycobacteria have been isolated from wild and captive amphibians. in captive anurans, cutaneous and visceral mycobacteriosis are common and can result in significant mortality, particularly when animals are immunocompromised. mycobacterial arthritis and synovitis are reported rarely in amphibians. we describe 20 cases in painted reed frogs (hyperolius marmoratus), which presented with cachexia, limb paresis or paralysis or 'spindly leg syndrome'. histopathology reveal ... | 2017 | 28233523 |
| epidemiology of nontuberculous mycobacterial lung disease and tuberculosis, hawaii, usa. | previous studies found hawaiians and asian-americans/pacific islanders to be independently at increased risk for nontuberculous mycobacterial pulmonary disease (ntmpd) and tuberculosis (tb). to better understand ntm infection and tb risk patterns in hawaii, usa, we evaluated data on a cohort of patients in hawaii for 2005-2013. period prevalence of ntmpd was highest among japanese, chinese, and vietnamese patients (>300/100,000 persons) and lowest among native hawaiians and other pacific islande ... | 2017 | 28221128 |
| pulmonary nontuberculous mycobacteria-associated deaths, ontario, canada, 2001-2013. | survival implications of nontuberculous mycobacterial pulmonary disease (ntm-pd) and ntm pulmonary isolation without disease (ntm-pi) are unclear. to study deaths associated with ntm-pd and ntm-pi and differences in survival between them, we conducted a population-based cohort study of persons with microbiologically defined ntm-pd or ntm-pi diagnosed during 2001-2013 in ontario, canada. we used propensity score matching and cox proportional hazards models to compare survival. among 9,681 ntm-pd ... | 2017 | 28221106 |
| high incidence of non-tuberculous mycobacteria-positive cultures among adolescent with cystic fibrosis. | we evaluated the prevalence of non-tuberculous mycobacteria (ntm)-positive cultures among our cystic fibrosis (cf) center patients, reviewed risk factors for ntm positivity, and determined its impact on lung function evolution. | 2017 | 28202251 |
| endobronchial lesions of mycobacterium abscessus infection in an immunocompromised patient. | | 2017 | 28199133 |
| detection of mycobacterium chelonae, mycobacterium abscessus group, and mycobacterium fortuitum complex by a multiplex real-time pcr directly from clinical samples using the bd max system. | a new multiplex pcr test was designed to detect mycobacterium chelonae, mycobacterium abscessus group, and mycobacterium fortuitum complex on the bd max system. a total of 197 clinical samples previously submitted for mycobacterial culture were tested using the new protocol. samples were first treated with proteinase k, and then each sample was inoculated into the bd max sample buffer tube. extraction and multiplex pcr were performed by the bd max system, using the bd max exk tna-3 extraction ki ... | 2017 | 28190461 |
| in vitro activity of clarithromycin in combination with other antimicrobial agents against mycobacterium abscessus and mycobacterium massiliense. | macrolides, especially clarithromycin (cla), remain the cornerstone of therapy for mycobacterium abscessus complex infections. the purpose of this study was to gather results from in vitro drug susceptibility testing of m. abscessus and mycobacterium massiliense for the combination of cla with various other agents, including linezolid (lzd), moxifloxacin (mox), amikacin (amk) and tigecycline (tgc). a total of 40 m. abscessus complex isolates were studied, comprising 20 m. abscessus and 20 m. mas ... | 2017 | 28188830 |
| mixed infection of mycobacterium abscessus subsp. abscessus and mycobacterium tuberculosis in the lung. | a mixed infection of mycobacterium abscessus subsp. abscessus (mab) and mycobacterium tuberculosis (mtb) in the lung is an unusual clinical manifestation and has not yet been reported. a 61-year-old woman had been treated for mab lung disease and concomitant pneumonia, and was diagnosed with pulmonary tuberculosis (ptb). despite both anti-ptb and anti-mab therapy, her entire left lung was destroyed and collapsed. she underwent left pneumonectomy and received medical therapy. we were able to succ ... | 2017 | 28180105 |
| case report on pulmonary disease due to coinfection of mycobacterium tuberculosis and mycobacterium abscessus: difficulty in diagnosis. | mycobacterium abscessus, which is ubiquitous environmental organism, is more likely to cause pulmonary infection in the presence underlying lung disease and immunosuppression. we report a case of pulmonary disease due to coinfection of mycobacterium tuberculosis (mtb) and mycobacterium abscessus (m. abscessus) in an immunocompetent patient without underlying lung disease. healthcare professionals should be aware of co-infection with mtb and m. abscessus, and treatment should be based on clinical ... | 2017 | 28180066 |
| a case of mycobacterium abscessus subsp. massiliense lung disease complicated by lipoid pneumonia. | | 2017 | 28157481 |
| structure-guided, target-based drug discovery - exploiting genome information from hiv to mycobacterial infections. | the use of protein crystallography in structure-guided drug discovery allows identification of potential inhibitor-binding sites and optimisation of interactions of hits and lead compounds with a target protein. an early example of this approach was the use of the structure of hiv protease in designing aids antivirals. more recently, use of structure-guided design with fragment-based drug discovery, which reduces the size of screening libraries by decreasing complexity, has improved ligand effic ... | 2016 | 28132480 |
| nontuberculous mycobacteria-overview. | nontuberculous mycobacteria (ntm) are emerging pathogens that affect both immunocompromised and immunocompetent patients. the development of molecular methods has allowed the characterization of new species and the identification of ntm to the precise species and subspecies levels. the incidence and prevalence of ntm lung disease are increasing worldwide, and this syndrome accounts for the majority of clinical cases of ntm disease. common causative organisms of pulmonary infection are the slowly ... | 2017 | 28128073 |
| notification of nontuberculous mycobacteria: an australian perspective. | in queensland, australia, all cases of mycobacterial infection (tuberculosis [tb] and nontuberculous mycobacteria [ntm]) are notifiable under the queensland public health act (2005). this process originally emerged to avoid ntm confounding with notification of cases of tb, but has facilitated awareness of the increasing incidence and changing epidemiology of ntm. although initially not a public health priority, the notification process has facilitated research that has led to an appreciation of ... | 2017 | 28118021 |
| resistance to thiacetazone derivatives active against mycobacterium abscessus involves mutations in the mmpl5 transcriptional repressor mab_4384. | available chemotherapeutic options are very limited against mycobacterium abscessus, which imparts a particular challenge in the treatment of cystic fibrosis (cf) patients infected with this rapidly growing mycobacterium. new drugs are urgently needed against this emerging pathogen, but the discovery of active chemotypes has not been performed intensively. interestingly, however, the repurposing of thiacetazone (tac), a drug once used to treat tuberculosis, has increased following the decipherin ... | 2017 | 28096157 |
| inhibition of the β-lactamase blamab by avibactam improves the in vitro and in vivo efficacy of imipenem against mycobacterium abscessus. | mycobacterium abscessus pulmonary infections are treated with a macrolide (clarithromycin or azithromycin), an aminoglycoside (amikacin), and a β-lactam (cefoxitin or imipenem). the triple combination is used without any β-lactamase inhibitor, even though mabscessus produces the broad-spectrum β-lactamase blamab we determine whether inhibition of blamab by avibactam improves the activity of imipenem against m. abscessus the bactericidal activity of drug combinations was assayed in broth and in h ... | 2017 | 28096155 |
| two-phase hospital-associated outbreak of mycobacterium abscessus: investigation and mitigation. | nontuberculous mycobacteria (ntm) commonly colonize municipal water supplies and cause healthcare-associated outbreaks. we investigated a biphasic outbreak of mycobacterium abscessus at a tertiary care hospital. | 2017 | 28077517 |
| trfa1 gene in clinical isolates of mycobacterium tuberculosis. | the incidence of resistant strains of mycobacterium tuberculosis (mtb), including multi-drug resistant, extensively drug resistant, or totally drug resistant, is one of the major problems of health policies worldwide. the accumulation of mutations causes multi-drug resistant strains. mycobacterium abscessus has a plasmid called pmab2401 containing the trfa1 gene in its integron part. the aim of the present study was to investigate the possible existence of the trfa1 gene in clinical strains of m ... | 2016 | 28043564 |
| identification of nontuberculous mycobacteria by high-performance liquid chromatography from patients in tehran. | the genus mycobacterium contains over 140 species comprises pathogenic and nonpathogenic strains. nontuberculous mycobacteria (ntm) causing clinical disease have become increasingly common and more diverse. widespread features of ntm infection can make the diagnosis difficult. precise species-level detection can aid in distinguishing environmental contamination from actual infection and, furthermore, can assist in making a choice of antimicrobial therapy. | 2016 | 28043562 |
| progression and treatment outcomes of lung disease caused by mycobacterium abscessus and mycobacterium massiliense. | mycobacterium abscessus and mycobacterium massiliense are grouped as the mycobacterium abscessus complex. the aim of this study was to elucidate the differences between m. abscessus and m. massiliense lung diseases in terms of progression rate, treatment outcome, and the predictors thereof. | 2017 | 28011609 |
| mycobacterial characteristics and treatment outcomes in mycobacterium abscessus lung disease. | treatment outcomes of patients with mycobacterium abscessus subspecies abscessus lung disease are poor, and the microbial characteristics associated with treatment outcomes have not been studied systematically. the purpose of this study was to identify associations between microbial characteristics and treatment outcomes in patients with m. abscessus lung disease. | 2017 | 28011608 |