Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| nontuberculous mycobacterial (ntm) lung disease: the top ten essentials. | this review will utilize essential questions about nontuberculous mycobacterial (ntm) lung disease to succinctly address important new developments in the pathogenesis, diagnosis and management of ntm lung disease with a focus on practical information and "bottom line" answers. 1) what do i tell my patients who ask, “where did i get this infection” and, “should i take showers”? 2) what is the connection between bronchiectasis and the acquisition of ntm lung infection? 3) what other factors are i ... | 2014 | 24484653 |
| antibiotic treatment for nontuberculous mycobacteria lung infection in people with cystic fibrosis. | nontuberculous mycobacteria are mycobacteria, other than those in the mycobacterium tuberculosis complex, and are commonly found in the environment. nontuberculous mycobacteria species (most commonly mycobacterium avium complex and mycobacterium abscessus) are isolated from the respiratory tract of approximately 5% to 20% of individuals with cystic fibrosis; they can cause lung disease in people with cystic fibrosis leading to more a rapid decline in lung function and even death in certain circu ... | 2014 | 25470304 |
| infections caused by non-tuberculous mycobacteria in recipients of hematopoietic stem cell transplantation. | non-tuberculous mycobacteria (ntm) are acid-fast bacteria that are ubiquitous in the environment and can colonize soil, dust particles, water sources, and food supplies. they are divided into rapidly growing mycobacteria such as mycobacterium fortuitum, mycobacterium chelonae, and mycobacterium abscessus as well as slowly growing species such as mycobacterium avium, mycobacterium kansasii, and mycobacterium marinum. about 160 different species, which can cause community acquired and health care- ... | 2014 | 25426446 |
| [identification of mycobacterium spp. isolates using matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (maldi-tof ms)]. | matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (maldi-tof ms) has recently been widely used in diagnostic microbiological laboratories. it is a cheap and rapid method for the identification of bacteria and micromycetes. apart from this purpose, it is also used for the detection of antibiotic resistance mechanisms. it has the potential to be extended for other purposes in microbiology. the aim of this study was to validate maldi-tof ms for the identification of mycob ... | 2014 | 25412483 |
| fourth death caused by mycobacterium abscessus reported at south carolina hospital. | 2014 | 25383413 | |
| structural and functional characterization of an arylamine n-acetyltransferase from the pathogen mycobacterium abscessus: differences from other mycobacterial isoforms and implications for selective inhibition. | mycobacterium abscessus is the most pathogenic rapid-growing mycobacterium and is one of the most resistant organisms to chemotherapeutic agents. however, structural and functional studies of m. abscessus proteins that could modify/inactivate antibiotics remain nonexistent. here, the structural and functional characterization of an arylamine n-acetyltransferase (nat) from m. abscessus [(mycab)nat1] are reported. this novel prokaryotic nat displays significant n-acetyltransferase activity towards ... | 2014 | 25372695 |
| respiratory review of 2014: tuberculosis and nontuberculous mycobacterial pulmonary disease. | since tuberculosis (tb) remains a major global health concern and the incidence of multi-drug resistant (mdr)-tb is increasing globally, new modalities for the detection of tb and drug resistant tb are needed to improve tb control. the xpert mtb/rif test can be a valuable new tool for early detection of tb and rifampicin resistance, with a high sensitivity and specificity. late-generation fluoroquinolones, levofloxacin, and moxifloxacin, which are the principal drugs for the treatment of mdr-tb, ... | 2014 | 25368661 |
| mycobacterium abscessus subsp abscessus lung disease: 'trouble ahead, trouble behind…'. | mycobacterium abscessus subsp abscessus is the most common respiratory pathogen among the rapidly growing non-tuberculous mycobacteria (ntm) and is also the most feared due to its well-deserved reputation for being refractory to antibiotic therapy. m. abscessus subsp abscessus has multiple innate antibiotic resistance mechanisms, but the most important one described so far is an inducible erythromycin methylase (erm) gene. m. abscessus subsp abscessus isolates may appear macrolide susceptible on ... | 2014 | 25580261 |
| vertebral osteomyelitis caused by mycobacterium abscessus surgically treated using antibacterial iodine-supported instrumentation. | mycobacterium abscessus infections rarely develop in healthy individuals, and mostly they occur in immunocompromised hosts. vertebral osteomyelitis due to mycobacterium abscessus is very rare and only three previous cases of spinal infection caused by mycobacterium abscessus have been reported. mycobacterium abscessus isolates are uniformly resistant to antituberculous agents and can display a virulent biofilm-forming phenotype. the patient was a 67-year-old woman with vertebral osteomyelitis of ... | 2014 | 25544922 |
| drug susceptibility testing of nontuberculous mycobacteria. | diseases caused by nontuberculous mycobacteria are emerging in many settings. with an increased number of patients needing treatment, the role of drug susceptibility testing is again in the spotlight. this articles covers the history and methodology of drug susceptibility tests for nontuberculous mycobacteria, but focuses on the correlations between in vitro drug susceptibility, pharmacokinetics and in vivo outcomes of treatment. among slow-growing nontuberculous mycobacteria, clear correlations ... | 2014 | 25340838 |
| [mycobacterial lung disease in patients with cystic fibrosis--report of three cases]. | mycobacterial lung disease is caused by nontuberculous mycobacteria (ntm), also known as atypical mycobacteria. ntm are widely distributed in the environment, particularly in soil and water. although generally of low pathogenicity to humans, ntm can affect patients with underlying chronic lung diseases, such as cystic fibrosis, bronchiectasis, pneumoconiosis, or healed tuberculosis. some patients with cystic fibrosis (cf) have disease progression due to ntm, others can have ntm cultured intermit ... | 2014 | 25339567 |
| management of mycobacterium abscessus post abdominoplasty. | atypical mycobacterium is the source of a recent outbreak of cosmetic surgery infections [1]. such an infection is difficult to eradicate and presents unique challenges in its management after esthetic surgery. delays in appropriate treatment are frequent because of difficulty in diagnosis and unfamiliarity with this type of infection. we present cases of our management of mycobacterial abscessus abdominoplasty infections following cosmetic surgery in the dominican republic. rapid initial recogn ... | 2014 | 25338711 |
| mycobacterium abscessus lung disease in a patient with kartagener syndrome. | primary ciliary dyskinesia (pcd) is characterized by the congenital impairment of mucociliary clearance. when accompanied by situs inversus, chronic sinusitis and bronchiectasis, pcd is known as kartagener syndrome. the main consequence of impaired ciliary function is a reduced mucus clearance from the lungs, and susceptibility to chronic respiratory infections due to opportunistic pathogens, including nontuberculous mycobacteria (ntm). there has been no report of ntm lung disease combined with ... | 2014 | 25309609 |
| importance of reciprocal balance of t cell immunity in mycobacterium abscessus complex lung disease. | little is known about the nature of the host immune response to mycobacterium abscessus complex (mabc) infection. the aim of the present study was to investigate whether alterations in serum immunomolecule levels after treating mabc lung disease patients with antibiotics can reflect the disease-associated characteristics. | 2014 | 25295870 |
| a rare native mitral valve endocarditis successfully treated after surgical correction. | mycobacterium abscessus and kocuria species are rare causes of infections in humans. endocarditis by these agents has been reported in only 11 cases. m. abscessus is a particularly resistant organism and treatment requires the association of antibiotics for a prolonged period of time. we report a case of native mitral valve bacterial endocarditis due to m. abscessus and kocuria species in a 48-year-old man with a history of intravenous drug use. the case was complicated by a perforation of the p ... | 2014 | 25270154 |
| mycobacterium abscessus: challenges in diagnosis and treatment. | mycobacterium abscessus is the most common rapidly growing mycobacterium that causes lung disease. this review describes recently published literature regarding m. abscessus taxonomy, environmental niche, diagnosis, management and outcome in pulmonary disease in adults and adolescents with cystic fibrosis. | 2014 | 25268925 |
| delivery of aerosolized liposomal amikacin as a novel approach for the treatment of nontuberculous mycobacteria in an experimental model of pulmonary infection. | pulmonary infections caused by nontuberculous mycobacteria (ntm) are an increasing problem in individuals with chronic lung conditions and current therapies are lacking. we investigated the activity of liposomal amikacin for inhalation (lai) against ntm in vitro as well as in a murine model of respiratory infection. macrophage monolayers were infected with three strains of mycobacterium avium, two strains of mycobacterium abscessus, and exposed to lai or free amikacin for 4 days before enumerati ... | 2014 | 25264757 |
| [the analysis of species identification and susceptibility testing of mycobacterium abscessus]. | to evaluate the method of differentiating mycobacterium abscessus subsp. abscessus (subsp. m. abscessus) from mycobacterium abscessus subsp. massiliense (subsp. m. massiliense), and to investigate the activity of different antibiotics in vitro. | 2014 | 25262693 |
| [subspecies identification for mycobacterium abscessus group]. | to identify the subspecies of mycobacterium abscessus (m.abscessus) group. | 2014 | 25262692 |
| [lipotourism, not without risks: a complication of cosmetic surgery abroad]. | postoperative infections with non-tuberculous mycobacteria (ntm) are rare. early diagnosis is important so tailored antibiotic therapy can be started. this can prevent unnecessary radiological and surgical interventions. | 2014 | 25248735 |
| gradenigo syndrome caused by nontuberculous mycobacteria. | gradenigo syndrome is a rare but devastating complication of otitis media that involves the petrous apex. clinically, it is characterized by the triad of suppurative otitis media, deep facial pain, and abducens palsy. most of the gradenigo syndrome cases that have been reported in the literature were caused by pyogenic bacteria. in this report, we describe the clinical courses of 4 adults with gradenigo syndrome who were encountered consecutively at a tertiary referral hospital between 2008 and ... | 2014 | 25247278 |
| a shorter treatment duration may be sufficient for patients with mycobacterium massiliense lung disease than with mycobacterium abscessus lung disease. | mycobacterium abscessus complex is the second most common organism isolated from patients with nontuberculous mycobacterial (ntm) lung disease in south korea. this study aimed to compare clinical features and treatment outcomes of m. abscessus and mycobacterium massiliense lung disease. | 2014 | 25245792 |
| pulmonary non-tuberculous mycobacteria in a general respiratory population. | the prevalence of non-tuberculous mycobacterium (ntm) appears to be increasing. much of the experience in the literature about this emerging organism comes from specialised units or populations such as cystic fibrosis patients. we, therefore, aim to evaluate the experience in a general respiratory population of dealing with patients with positive culture of ntm. we did a retrospective review of medical notes of general respiratory patients from whom ntm were isolated from january 2007 to july 20 ... | 2014 | 25226715 |
| mycobacteriophage-drived diversification of mycobacterium abscessus. | mycobacterium abscessus is an emerging opportunistic pathogen which diversity was acknowledged by the recent description of two subspecies accommodating m. abscessus, mycobacterium bolletii and mycobacterium massiliense isolates. | 2014 | 25224692 |
| advances in the management of pulmonary disease due to mycobacterium abscessus complex. | mycobacterium abscessus complex is a group of rapidly growing mycobacteria, and an emerging cause of non-tuberculous mycobacterial lung disease in patients with cystic fibrosis and chronic lung diseases, such as bronchiectasis. m. abscessus complex is the most drug-resistant of the mycobacterial pathogens, resulting in limited therapeutic options and a high treatment failure rate. m. abscessus complex is comprised of three closely related subspecies: m. abscessus (sensu stricto), m. massiliense ... | 2014 | 25216826 |
| in vitro evaluation of a new drug combination against clinical isolates belonging to the mycobacterium abscessus complex. | the in vitro susceptibility profile to amikacin, linezolid, clarithromycin, imipenem, cefoxitin, clofazimine and tigecycline was established for 67 strains belonging to the mycobacterium abscessus complex. clofazimine and tigecycline were among the most effective drugs, prompting us to assess the effect of a clofazimine and tigecycline combination. synergistic activity was found in 42% of the 19 isolates tested. the clinical impact of this new drug combination against the m. abscessus complex, a ... | 2014 | 25185732 |
| a surface with a biomimetic micropattern reduces colonization of mycobacterium abscessus. | nontuberculous mycobacteria (ntm) are ubiquitous organisms found in soil, water, and biofilms. engineered surface topography has been proposed as a method to reduce microbial biofilm formation. the sharklet(®) micropattern silicone surface has been shown to reduce biofilm formation of pyogenic bacteria. we hypothesized that this micropattern surface will also reduce colonization by mycobacterium abscessus, a human pathogen. smooth and micropattern silicone samples were incubated with 1 × 10(6) m ... | 2014 | 25155501 |
| multilocus sequence typing scheme versus pulsed-field gel electrophoresis for typing mycobacterium abscessus isolates. | outbreaks of infections by rapidly growing mycobacteria following invasive procedures, such as ophthalmological, laparoscopic, arthroscopic, plastic, and cardiac surgeries, mesotherapy, and vaccination, have been detected in brazil since 1998. members of the mycobacterium chelonae-mycobacterium abscessus group have caused most of these outbreaks. as part of an epidemiological investigation, the isolates were typed by pulsed-field gel electrophoresis (pfge). in this project, we performed a large- ... | 2014 | 24899019 |
| soft tissue infection caused by rapid growing mycobacterium following medical procedures: two case reports and literature review. | non-tubecrulosis mycobacterium infections were increasingly reported either pulmonary or extrapulmonary in the past decades. in taiwan, we noticed several reports about the soft tissue infections caused by rapid growing mycobacterium such as mycobacterium abscessus, mycobacterium chelonae, on newspaper, magazines, or the multimedia. most of them occurred after a plastic surgery, and medical or non-medical procedures. here, we reported two cases of these infections following medical procedures. w ... | 2014 | 24882980 |
| identification of mycobacterium species following growth detection with the bactec mgit 960 system by dna line probe assay. | the tuberculosis and infections caused by nontuberculous mycobacterial (ntm) species are increasing in patients presented with respiratory illness, and it is crucial to document the epidemiology of these infections. | 2014 | 26786328 |
| presentation of mycobacterium abscessus infection following rhytidectomy to a uk plastic surgery unit. | we report the presentation of a patient to a uk plastic surgery unit with mycobacterium abscessus infection following a facelift surgery in southern india. treatment was protracted requiring surgical debridement and 6 months of antibiotics including a 3-week hospital admission for intravenous antibiotic therapy. we describe the clinical presentation, diagnosis and treatment of this unusual microorganism with reference to more familiar pyogenic infections. | 2014 | 24872486 |
| recurrent nontuberculous mycobacterial endophthalmitis: a diagnostic conundrum. | to report a case of recurrent nontuberculous mycobacterial endophthalmitis in the context of neurotrophic keratopathy secondary to herpes zoster ophthalmicus that had an atypical presentation and complex course, and highlights the challenges of causative organism identification and therapeutic interventions in this condition. | 2014 | 24833891 |
| highlights of the north american cf conference 2013. | this is a personal selection of papers that were presented at the 27th north american cystic fibrosis conference held in salt lake city in october 2013. the papers discussed in this review are thought to be of particular interest to cf caregivers in the uk. | 2014 | 24821547 |
| rapid identification of strains belonging to the mycobacterium abscessus group through erm(41) gene pyrosequencing. | mycobacterium abscessus and mycobacterium massiliense lung infections have different clarithromycin susceptibilities, making proper identification important; however, standard multi-gene sequencing in clinical laboratories is laborious and time consuming. we developed a pyrosequencing-based method for rapid identification of strains belonging to the m. abscessus group by targeting erm(41). we examined 55 isolates from new pulmonary m. abscessus infections and identified 28 m. abscessus, 25 m. ma ... | 2014 | 24809859 |
| in vivo assessment of drug efficacy against mycobacterium abscessus using the embryonic zebrafish test system. | mycobacterium abscessus is responsible for a wide spectrum of clinical syndromes and is one of the most intrinsically drug-resistant mycobacterial species. recent evaluation of the in vivo therapeutic efficacy of the few potentially active antibiotics against m. abscessus was essentially performed using immunocompromised mice. herein, we assessed the feasibility and sensitivity of fluorescence imaging for monitoring the in vivo activity of drugs against acute m. abscessus infection using zebrafi ... | 2014 | 24798271 |
| [case report; disseminated mycobacterium abscessus infection with hemophagocytic syndrome during treatment of chronic lymphocytic leukemia]. | 2014 | 24796145 | |
| mycobacterium abscessus hand-and-foot disease in children: rare or emerging disease? | mycobacterium abscessus is emerging as an important cause of cutaneous infections in sporadic cases and outbreak settings. although immunosuppressed or elderly patients are most commonly affected, in 2006 an outbreak of clinically distinct cutaneous lesions on the hands and feet caused by m. abscessus in a population of healthy children using a public swimming pool was reported. this article describes an outbreak of skin infection in a population of healthy italian children attending the same sc ... | 2014 | 24758202 |
| surgical treatment of nontuberculous mycobacterial lung disease. | while the prevalence of pulmonary tuberculosis has been decreasing, the prevalence of nontuberculous mycobacterial lung disease has been increasing. unlike tuberculosis, nontuberculous mycobacterial disease is not communicable. however, their indolent nature may result in extensive parenchymal destruction, causing respiratory failure and vulnerability to airway infection. nontuberculous mycobacterial lung disease, therefore, has been becoming a significant health problem. according to the 2007 a ... | 2014 | 24740640 |
| gene replacement in mycobacterium chelonae: application to the construction of porin knock-out mutants. | mycobacterium chelonae is a rapidly growing mycobacterial opportunistic pathogen closely related to mycobacterium abscessus that causes cornea, skin and soft tissue infections in humans. although m. chelonae and the emerging mycobacterial pathogen m. abscessus have long been considered to belong to the same species, these two microorganisms considerably differ in terms of optimum growth temperature, drug susceptibility, pathogenicity and the types of infection they cause. the whole genome sequen ... | 2014 | 24739882 |
| cefoxitin continuous infusion for lung infection caused by the mycobacterium abscessus group. | 2014 | 24733469 | |
| mycobacterium tuberculosis pili (mtp), a putative biomarker for a tuberculosis diagnostic test. | novel biomarkers are urgently needed for point of care tb diagnostics. in this study, we investigated the potential of the pilin subunit protein encoded by the mtp gene as a diagnostic biomarker. blast analysis of the mtp gene on published genome databases, and amplicon sequencing were performed in mycobacterium tuberculosis complex (mtbc) strains and other organisms. the protein secondary structure of the amino acid sequences of non-tuberculous mycobacteria that partially aligned with the mtp s ... | 2014 | 24721207 |
| clonal relationship and differentiation among mycobacterium abscessus isolates as determined using the semiautomated repetitive extragenic palindromic sequence pcr-based diversilab system. | mycobacterium abscessus is a rapidly growing mycobacterium that causes respiratory tract infections in predisposed patients, such as those with cystic fibrosis and nosocomial skin and soft tissue infections. in order to investigate the clonal relationships between the strains causing epidemic episodes, we evaluated the discriminatory power of the semiautomated diversilab (dl) repetitive extragenic palindromic sequence pcr (rep-pcr) test for m. abscessus genotyping. since m. abscessus was shown t ... | 2014 | 24671795 |
| mycobacterium abscessus mab2560 induces maturation of dendritic cells via toll-like receptor 4 and drives th1 immune response. | in this study, we showed that mycobacterium abscessus mab2560 induces the maturation of dendritic cells (dcs), which are representative antigen-presenting cells (apcs). m. abscessus mab2560 stimulate the production of pro-inflammatory cytokines [interleukin (il)-6, tumor necrosis factor (tnf)-α, il-1β, and il-12p70] and reduce the endocytic capacity and maturation of dcs. using tlr4-/- dcs, we found that mab2560 mediated dc maturation via toll-like receptor 4 (tlr4). mab2560 also activated the m ... | 2014 | 24667171 |
| outbreaks due to mycobacterium abscessus subsp. bolletii in southern brazil: persistence of a single clone from 2007 to 2011. | outbreaks associated with rapidly growing mycobacteria (rgm) have been increasingly reported worldwide, including in brazil. among the rgm, the mycobacterium abscessus complex is the most pathogenic and related to multidrug resistance. the aim of this study was to evaluate the antimicrobial susceptibility and molecular profile of rgm isolates involved in new postsurgical infection outbreaks in brazil since 2007. of the 109 cases reported in the state of rio grande do sul between 2007 and 2011, 4 ... | 2014 | 25038135 |
| species identification of mycobacterium abscessus subsp. abscessus and mycobacterium abscessus subsp. bolletii using rpob and hsp65, and susceptibility testing to eight antibiotics. | to separate mycobacterium abscessus subsp. bolletii from mycobacterium abscessus subsp. abscessus using species identification, and to investigate the in vitro activity of amikacin, cefoxitin, imipenem, levofloxacin, moxifloxacin, clarithromycin, azithromycin, and linezolid against mycobacterium abscessus. | 2014 | 24932856 |
| multidrug-resistant nontuberculous mycobacteria isolated from cystic fibrosis patients. | worldwide, nontuberculous mycobacteria (ntm) have become emergent pathogens of pulmonary infections in cystic fibrosis (cf) patients, with an estimated prevalence ranging from 5 to 20%. this work investigated the presence of ntm in sputum samples of 129 cf patients (2 to 18 years old) submitted to longitudinal clinical supervision at a regional reference center in rio de janeiro, brazil. from june 2009 to march 2012, 36 ntm isolates recovered from 10 (7.75%) out of 129 children were obtained. mo ... | 2014 | 24920766 |
| bacteraemia caused by mycobacterium abscessus subsp. abscessus and m. abscessus subsp. bolletii: clinical features and susceptibilities of the isolates. | mycobacterium abscessus complex (m. abscessus subsp. abscessus and m. abscessus subsp. bolletii) is an emerging pathogen causing various human infections. however, few studies have focused on m. abscessus complex bacteraemia with detailed species differentiation. the clinical characteristics of patients with bacteraemia due to m. abscessus complex treated at national taiwan university hospital from 2005-2012 were evaluated. species identification was performed by molecular methods, and minimum i ... | 2014 | 24718088 |
| demonstration of plasmid-mediated drug resistance in mycobacterium abscessus. | plasmid-mediated kanamycin resistance was detected in a strain of mycobacterium abscessus subsp. bolletii responsible for a nationwide epidemic of surgical infections in brazil. the plasmid did not influence susceptibility to tobramycin, streptomycin, trimethoprim-sulfamethoxazole, clarithromycin, or ciprofloxacin. plasmid-mediated drug resistance has not been described so far in mycobacteria. | 2014 | 24574286 |
| the first korean case of nontuberculous mycobacterial lung disease caused by mycobacterium abscessus subspecies bolletii in a patient with bronchiectasis. | we report the first korean case of lung diseases caused by mycobacterium abscessus subsp. bolletii in a previously healthy male, except for a previous history of pulmonary tuberculosis and bronchiectasis. all serial isolates are identified as m. abscessus subsp. bolletii by multi-locus sequence analysis based on the hsp65, rpob, and 16s rrna fragments. at the genetic level, the isolate has the erm(41) gene with a t28 sequevar, associated with clarithromycin resistance, and no rrl mutation. the i ... | 2014 | 24523815 |
| mycobacterium abscessus complex lung infection in a toddler with a tracheostomy. | mycobacterium abscessus complex and other non-tuberculous mycobacteria are infrequently encountered respiratory pathogens in patients with tracheostomies. we report a 4-year-old girl with a tracheostomy, placed during infancy for management of severe bronchopulmonary dysplasia and laryngeal stenosis, who developed a m. abscessus complex lung infection. there was clear evidence of parenchymal involvement and true infection beyond colonization. she demonstrated dramatic clinical, laboratory, and r ... | 2014 | 23460506 |
| mixed mycobacterial infection in an adult koi carp cyprinus carpio l. | 2014 | 23937602 | |
| complete genome sequence and comparative genomic analysis of mycobacterium massiliense jcm 15300 in the mycobacterium abscessus group reveal a conserved genomic island mmgi-1 related to putative lipid metabolism. | mycobacterium abscessus group subsp., such as m. massiliense, m. abscessus sensu stricto and m. bolletii, are an environmental organism found in soil, water and other ecological niches, and have been isolated from respiratory tract infection, skin and soft tissue infection, postoperative infection of cosmetic surgery. to determine the unique genetic feature of m. massiliense, we sequenced the complete genome of m. massiliense type strain jcm 15300 (corresponding to ccug 48898). comparative genom ... | 2014 | 25503461 |
| risk factors for mycobacterium abscessus subsp. bolletii infection after laparoscopic surgery during an outbreak in brazil. | objective to identify risk factors related to mycobacterium abscessus subsp. bolletii infection during an outbreak, associated with laparoscopic surgery and to propose recommendations for preventing new cases. design a retrospective cohort study. setting a private hospital in manaus, brazil. patients a cohort of 222 patients who underwent laparoscopic surgery between july 2009 and august 2010 by a single surgical team. methods we collected information about the patients and the surgical procedur ... | 2015 | 25627765 |
| inducible and acquired clarithromycin resistance in the mycobacterium abscessus complex. | clarithromycin was considered the cornerstone for the treatment of mycobacterium abscessus complex infections. genetic resistance mechanisms have been described and many experts propose amikacin as an alternative. nevertheless, clarithromycin has several advantages; therefore, it is necessary to identify the non-functional erm(41) allele to determine the most suitable treatment. the aims of this study were to characterize the molecular mechanisms of clarithromycin resistance in a collection of m ... | 2015 | 26448181 |
| intracranial mycobacterium abscessus infection in a healthy toddler. | we present the first case of pediatric intracranial mycobacterium abscessus infection in a 16-month-old female with neurofibromatosis type 1. we describe a successful treatment regimen including excisional biopsy combined with high-dose steroids and 16 weeks of triple antimicrobial therapy that resulted in clinical cure and an excellent neurologic outcome. | 2015 | 25144796 |
| caspase-independent apoptosis in infected macrophages triggered by sulforaphane via nrf2/p38 signaling pathways. | mycobacterium abscessus (mabs), a non-tuberculous mycobacterium, is an emerging and rapidly growing opportunistic pathogen that is frequently found in patients with cystic fibrosis and in immunosuppressed patients. its high tolerance to antibiotics is of great concern for public health. in this study, our results showed that human thp-1-derived macrophages infected with m. abscessus presented an increase in ros production and cell necrosis. in addition, m. abscessus infection triggered activatio ... | 2015 | 27551455 |
| nontuberculous mycobacteria: skin and soft tissue infections. | skin and soft tissue infections caused by nontuberculous mycobacteria are increasing in incidence. the nontuberculous mycobacteria are environmental, acid-fast bacilli that cause cutaneous infections primarily after trauma, surgery and cosmetic procedures. skin findings include abscesses, sporotrichoid nodules or ulcers, but also less distinctive signs. important species include mycobacterium marinum and the rapidly growing mycobacterium: m. fortuitum, m. abscessus and m. chelonae. obtaining tis ... | 2015 | 26143432 |
| inhaled antibiotics for refractory nontuberculous mycobacteria and non-cystic fibrosis bronchiectasis and the significance of mycobacterium abscessus subsp. abscessus isolation during m. avium complex lung disease therapy. | 2015 | 26131990 | |
| iatrogenic mycobacterium abscessus infection in a trigger finger. | an immunocompetent 63-year-old lady developed mycobacterium abscessus soft tissue infection of the hand following local corticosteroid injection for trigger finger. the patient was successfully treated with repeated radical debridement and prolonged antimicrobial therapy. atypical mycobacterial infections, including those caused by m. abscessus, albeit rare, should be considered in cases of late-onset indolent infection following local injury surgical procedures, and injections. clinical vigilan ... | 2015 | 26117482 |
| ifitms from mycobacteria confer resistance to influenza virus when expressed in human cells. | interferon induced transmembrane proteins (ifitms) found in vertebrates restrict infections by specific viruses. ifitm3 is known to be essential for restriction of influenza virus infections in both mice and humans. vertebrate ifitms are hypothesized to have derived from a horizontal gene transfer from bacteria to a primitive unicellular eukaryote. since bacterial ifitms share minimal amino acid identity with human ifitm3, we hypothesized that examination of bacterial ifitms in human cells would ... | 2015 | 26075508 |
| evaluation of pcr-reverse blot hybridization assay for the differentiation and identification of mycobacterium species in liquid cultures. | the purpose of this study was to evaluate the usefulness of reba myco-id(®) assay based on the principle of reverse blot hybridization assay (reba) for the rapid detection of mycobacterium tuberculosis (mtb), the differentiation of mtb from nontuberculous mycobacteria (ntm) and the identification of ntm species in liquid cultures. | 2015 | 25346202 |
| time-kill kinetics of antibiotics active against rapidly growing mycobacteria. | this study was conducted to generate basic pharmacodynamic information on the relationship between antibiotic concentrations and the growth of rapidly growing mycobacteria (rgm), and thereby contribute to a better understanding of current and future drug regimens for diseases caused by rgm. | 2015 | 25344808 |
| the drug susceptibility profile and inducible resistance to macrolides of mycobacterium abscessus and mycobacterium massiliense in korea. | we conducted drug susceptibility testing (dst) against various antimicrobial agents, including new candidate drugs, and investigated the relationship between inducible resistance (ir) to macrolides and erm(41) gene in mycobacterium abscessus complex. sixty-two isolates of m. abscessus complex from 2 tertiary care hospitals in south korea were tested against 10 antimicrobial agents. thirty-five isolates were m. abscessus, and 27 were mycobacterium massiliense. amikacin, moxifloxacin, linezolid, c ... | 2015 | 25467784 |
| whole-genome sequencing and epidemiological analysis do not provide evidence for cross-transmission of mycobacterium abscessus in a cohort of pediatric cystic fibrosis patients. | mycobacterium abscessus has emerged as a major pathogen in cystic fibrosis (cf) patients and has been associated with poor clinical outcomes, particularly following lung transplant. we investigated the acquisition of this bacterium in a cohort of pediatric cf patients. | 2015 | 25452595 |
| risk factors for microbiologic failure among taiwanese adults with mycobacterium abscessus complex pulmonary disease. | the mycobacterium abscessus complex is a common cause of pulmonary nontuberculous mycobacteria infections in taiwan. we examined the risk factors associated with treatment outcome in taiwanese adults with pulmonary disease caused by the m. abscessus complex. | 2015 | 25440976 |
| non-tuberculous mycobacterial cochlear implant infection: an emerging pathogen. | to report one of the first cases of a cochlear implant infected by non-tuberculous mycobacteria (ntm), an emerging otologic pathogen. | 2015 | 25747861 |
| rapid detection and immune characterization of mycobacterium abscessus infection in cystic fibrosis patients. | cystic fibrosis patients are highly susceptible to infections with non-tuberculous mycobacteria. especially mycobacterium abscessus infections are common but reliable diagnosis is hampered by non-specific clinical symptoms and insensitive mycobacterial culture. in the present study we established novel methods for rapid detection and immune characterization of mycobacterium abscessus infection in cystic fibrosis patients. we performed mycobacterium abscessus specific dna-strip- and quantitative ... | 2015 | 25742660 |
| impact of β-lactamase inhibition on the activity of ceftaroline against mycobacterium tuberculosis and mycobacterium abscessus. | the production of β-lactamases bla(mab) and blac contributes to β-lactam resistance in mycobacterium abscessus and mycobacterium tuberculosis, respectively. ceftaroline was efficiently hydrolyzed by these enzymes. inhibition of m. tuberculosis blac by clavulanate decreased the ceftaroline mic from ≥ 256 to 16 to 64 μg/ml, but these values are clinically irrelevant. in contrast, the ceftaroline-avibactam combination should be evaluated against m. abscessus since it inhibited growth at lower and p ... | 2015 | 25733512 |
| mycobacterium abscessus pneumonia in a south pacific islander. | 2015 | 25731702 | |
| lessons learnt from an atypical mycobacterium infection post-anterior cruciate ligament reconstruction. | infections following anterior cruciate ligament reconstruction are rare, with no previous reports citing mycobacterium abscessus as the culprit pathogen. a 22-year-old man presented twice over three years with a painful discharging sinus over his right tibia tunnel site necessitating repeated arthroscopy and washout, months of antibiotic therapy, and ultimately culminating in the removal of the implants. in both instances, m. abscessus was present in the wound cultures, along with a coinfection ... | 2015 | 25729530 |
| detection of mycobacterium abscessus from deep pharyngeal swabs in cystic fibrosis. | 2015 | 25727723 | |
| treatment of mycobacterium abscessus subsp. massiliense tricuspid valve endocarditis. | 2015 | 25695799 | |
| population-based incidence of pulmonary nontuberculous mycobacterial disease in oregon 2007 to 2012. | pulmonary nontuberculous mycobacteria (ntm) disease is a chronic, nonreportable illness, making it difficult to monitor. although recent studies suggest an increasing prevalence of ntm disease in the united states, the incidence and temporal trends are unknown. | 2015 | 25692495 |
| nontuberculous mycobacterial infections in cystic fibrosis. | nontuberculous mycobacteria (ntm) are important emerging cystic fibrosis (cf) pathogens. factors including the steady aging of the cf population, the apparent increase of ntm in the environment, and the potential for patient-to-patient transmission, may contribute to increased acquisition. diagnosis of ntm disease is challenging due to disease overlap; thus, comprehensive care of the cf patient must be optimized to assess the clinical impact of the ntm (indolent versus active), and to improve re ... | 2015 | 25676523 |
| nontuberculous mycobacteria infections in immunosuppressed hosts. | diseases and therapies that reduce cell-mediated immunity increase the risk of nontuberculous mycobacterial (ntm) disease. extrapulmonary ntm disease, including disseminated, skin, and catheter-related disease, is more common in immunosuppressed than immunocompetent patients. mycobacterium avium complex remains the most common cause of ntm infection, but rapid growers including mycobacterium abscessus, mycobacterium chelonae, and mycobacterium fortuitum play an important role in skin and cathete ... | 2015 | 25676522 |
| the treatment of rapidly growing mycobacterial infections. | rapidly growing mycobacteria (rgm) include a diverse group of species. we address the treatment of the most commonly isolated rgm-m abscessus complex, m fortuitum, and m chelonae. the m abscessus complex is composed of 3 closely related species: m abscessus senso stricto (hereafter m abscessus), m massiliense, and m bolletii. most studies address treatment of m abscessus complex, which accounts for 80% of lung disease caused by rgm and is the second most common rgm to cause extrapulmonary diseas ... | 2015 | 25676520 |
| preliminary results of bedaquiline as salvage therapy for patients with nontuberculous mycobacterial lung disease. | bedaquiline is an oral antimycobacterial agent belonging to a new class of drugs called diarylquinolines. it has low equivalent minimal inhibitory concentrations for mycobacterium tuberculosis and nontuberculous mycobacterial (ntm) lung disease, especially mycobacterium avium complex (mac) and mycobacterium abscessus (mab). bedaquiline appears to be effective for the treatment of multidrug-resistant tb but has not been tested clinically for ntm disease. | 2015 | 25675393 |
| mycobacterium abscessus morphotype comparison in a murine model. | pulmonary infections with mycobacterium abscessus (m. abscessus) are increasingly prevalent in patients with lung diseases such as cystic fibrosis. m. abscessus exists in two morphotypes, smooth and rough, but the impact of morphotype on virulence is unclear. we developed an immune competent mouse model of pulmonary m. abscessus infection and tested the differences in host inflammatory response between the morphotypes of m. abscessus. smooth and rough morphotypes of m. abscessus were isolated fr ... | 2015 | 25675351 |
| crystallization and preliminary x-ray diffraction analysis of a putative carbon-carbon bond hydrolase from mycobacterium abscessus 103. | the phlg protein from mycobacterium abscessus 103 (mphlg), which shares 30% sequence identity with phloretin hydrolase from eubacterium ramulus and 38% sequence identity with 2,4-diacetylphloroglucinol hydrolase from pseudomonas fluorescens pf-5, is a putative carbon-carbon bond hydrolase. here, the expression, purification and crystallization of mphlg are reported. crystals were obtained using a precipitant consisting of 100 mm citric acid ph 5.0, 1.0 m lithium chloride, 8%(w/v) polyethylene gl ... | 2015 | 25664803 |
| clinical significance of nontuberculous mycobacteria from respiratory specimens in stem cell transplantation recipients. | the clinical importance of the isolation of nontuberculous mycobacteria (ntm) from respiratory specimens of stem cell transplant (sct) recipients is not clear. we investigated the characteristics and clinical impact of ntm isolation in this population. medical records of adult patients who underwent sct at the blood and marrow transplantation center at a university hospital in south korea between january 2004 and december 2013 were retrospectively reviewed. twenty-five of 2,658 patients were enr ... | 2015 | 25663510 |
| rapidly growing mycobacteria in singapore, 2006-2011. | nontuberculous mycobacteria infection is a growing global concern, but data from asia are limited. this study aimed to describe the distribution and antibiotic susceptibility profiles of rapidly growing mycobacterium (rgm) isolates in singapore. clinical rgm isolates with antibiotic susceptibility tests performed between 2006 and 2011 were identified using microbiology laboratory databases and minimum inhibitory concentrations of amikacin, cefoxitin, clarithromycin, ciprofloxacin, doxycycline, i ... | 2015 | 25658536 |
| development of a peptide nucleic acid-based multiprobe real-time pcr method targeting the hsp65 gene for differentiation among mycobacterium abscessus strains. | recently, the need to distinguish between members of the mycobacterium abscessus group has gained increasing attention. here, we introduced a novel peptide nucleic acid (pna) real-time pcr method targeting the hsp65 gene in order to distinguish between four subspecies within the m. abscessus group (m. abscessus and 3 types of m. massiliense). | 2015 | 25653415 |
| utility of sequencing the erm(41) gene in isolates of mycobacterium abscessus subsp. abscessus with low and intermediate clarithromycin mics. | the erm(41) gene confers inducible macrolide resistance in mycobacterium abscessus subsp. abscessus, calling into question the usefulness of macrolides for treating m. abscessus subsp. abscessus infections. with an extended incubation (14 days), isolates with mics of ≥8 μg/ml are considered macrolide resistant by current clsi guidelines. our goals were to determine the incidence of macrolide susceptibility in u.s. isolates, the validity of currently accepted mic breakpoints, and the erm(41) sequ ... | 2015 | 25653399 |
| mycobacterium abscessus. "pleased to meet you, hope you guess my name...". | mycobacterium abscessus is a formidable and difficult-to-treat mycobacterial pathogen with multiple drug-resistance mechanisms. the most important of these mechanisms is the presence of an inducible erythromycin methylase (erm) gene, because it confers macrolide resistance. it has recently been found that "m. abscessus" can be split into three species or subspecies based on gene sequence analysis other than the 16s rrna gene and the presence or absence of a functional erm(41) gene. several names ... | 2015 | 25643064 |
| increasing patients with pulmonary mycobacterium avium complex disease and associated underlying diseases in japan. | this study was conducted to evaluate trends in the isolation of strains of nontuberculous mycobacteria (ntm) and trends in the number of patients with pulmonary mycobacterium avium complex (mac) disease. we retrospectively reviewed microbiological results and clinical data to identify patients who were diagnosed with pulmonary mac disease at kyoto university hospital in japan between 2000 and 2013. ntm were isolated from 6327 of 80,285 samples (7.9%) for mycobacterial culture. the proportion of ... | 2015 | 25640532 |
| induction of heme oxygenase-1 contributes to survival of mycobacterium abscessus in human macrophages-like thp-1 cells. | mycobacterium abscessus (m.abs) is a rapidly growing mycobacterial species that infects macrophages, and is an important pathogen in patients with cystic fibrosis. we studied the early stages of m.abs infection of macrophages, with emphasis on the role of heme-oxygenase-1 (ho-1) in this infection. thp-1 cells were activated using tpa into macrophage-like cells and infected with m.abs for different time points. m.abs infection robustly induced ho-1 expression in the thp-1 cells. production of ho- ... | 2015 | 25638774 |
| non-tuberculous mycobacterial infections after solid organ transplantation: a survival analysis. | the relationship of non-tuberculous mycobacterial (ntm) infections and survival among solid organ transplant recipients is unknown. we conducted a retrospective cohort study to measure the impact of ntm infection on survival in this patient population, comparing the effect of mycobacterium abscessus infection with that of infections due to other pathogenic ntm species. we identified 33 patients with ntm infection post-transplantation, 18 with infection that was diagnosed within the first year. a ... | 2015 | 25636926 |
| aminoglycoside-modifying enzymes determine the innate susceptibility to aminoglycoside antibiotics in rapidly growing mycobacteria. | infections caused by the rapidly growing mycobacterium (rgm) mycobacterium abscessus are notoriously difficult to treat due to the innate resistance of m. abscessus to most clinically available antimicrobials. aminoglycoside antibiotics (aga) are a cornerstone of antimicrobial chemotherapy against m. abscessus infections, although little is known about intrinsic drug resistance mechanisms. we investigated the role of chromosomally encoded putative aminoglycoside-modifying enzymes (ame) in aga su ... | 2015 | 25604746 |
| evaluation of the speed-oligo mycobacteria assay for the identification of nontuberculous mycobacteria. | nontuberculous mycobacteria (ntm) causing human infectious disease have become increasingly common. rapid and accurate identification to the species level is, therefore, critical. the speed-oligo mycobacteria assay is an oligochromatographic method that was made available recently for the identification and differentiation of mycobacteria. the present study aimed to evaluate the performance of the speed-oligo mycobacteria assay for the identification of ntm. we examined a total of 62 strains (9 ... | 2015 | 25596120 |
| investigation of the population structure of mycobacterium abscessus complex strains using 17-locus variable number tandem repeat typing and the further distinction of mycobacterium massiliense hsp65 genotypes. | mycobacterium abscessus complex is a significant pathogen in patients with non-cystic fibrosis (non-cf). nevertheless, there is little description of the genetic diversity of this species. the aims of this study were to investigate the distribution of m. abscessus complex isolated from respiratory specimens by variable number tandem repeat (vntr) typing. the results of 104 clinical isolates from 104 non-cf patients were compared using pfge, hsp65 genotypes and clarithromycin susceptibility. the ... | 2015 | 25596119 |
| nontuberculous mycobacterial ocular infections--comparing the clinical and microbiological characteristics between mycobacterium abscessus and mycobacterium massiliense. | to analyze the clinical characteristics of nontuberculous mycobacterial (ntm) ocular infections and the species-specific in vitro antimicrobial susceptibility. | 2015 | 25581038 |
| comparing mycobacterium massiliense and mycobacterium abscessus lung infections in cystic fibrosis patients. | mycobacterium massiliense is closely related to mycobacterium abscessus and is also a frequent cause of mycobacterial lung disease in patients with cystic fibrosis (cf). there has been no previous investigation of possible differences between m. massiliense and m. abscessus infections in the setting of cf. | 2015 | 25085077 |
| a novel inhibitor of gyrase b is a potent drug candidate for treatment of tuberculosis and nontuberculosis mycobacterial infections. | new drugs to treat drug-resistant tuberculosis are urgently needed. extensively drug-resistant and probably the totally drug-resistant tuberculosis strains are resistant to fluoroquinolones like moxifloxacin, which target gyrase a, and most people infected with these strains die within a year. in this study, we found that a novel aminobenzimidazole, vxc-486, which targets gyrase b, potently inhibits multiple drug-sensitive isolates and drug-resistant isolates of mycobacterium tuberculosis in vit ... | 2015 | 25534737 |
| several siblings with cystic fibrosis as a risk factor for poor outcome. | occurrence of cystic fibrosis (cf) in more than one member in a family is not uncommon. the aim of our study was to assess the influence of multiple siblings with cf on disease expression and outcome. | 2015 | 25528949 |
| β-lactamase inhibition by avibactam in mycobacterium abscessus. | two β-lactams, cefoxitin and imipenem, are part of the reference treatment for pulmonary infections with mycobacterium abscessus. m. abscessus has recently been shown to produce a broad-spectrum β-lactamase, blamab, indicating that the combination of β-lactams with a blamab inhibitor may improve treatment efficacy. the objectives of this study were to evaluate the impact of blamab production on the efficacy of β-lactams in vitro and to assess the benefit of blamab inhibition on the activity of β ... | 2015 | 25525201 |
| increasing nontuberculous mycobacteria infection in cystic fibrosis. | nontuberculous mycobacteria (ntm) are emerging infections in the cf population. | 2015 | 24917112 |
| the challenge of pulmonary nontuberculous mycobacterial infection. | the incidence of nontuberculous mycobacterial (ntm) lung disease is increasing. current treatment strategies are largely based on expert opinion. the lack of randomized clinical trials to inform treatment leave clinicians with many questions regarding the most effective and safe regimens. the risk-benefit ratio of therapy is often thought to favor observation given the chronic nature of the disease, multiple long-term antibiotics recommended for therapy, side effects associated with treatment, a ... | 2015 | 26877911 |
| post liposuction mycobacterium abscessus surgical site infection in a returned medical tourist complicated by a paradoxical reaction during treatment. | rapidly growing mycobacterial skin and soft tissue infections are known to complicate cosmetic surgical procedures. treatment consists of more surgery and prolonged antibiotic therapy guided by drug susceptibility testing. paradoxical reactions occurring during antibiotic therapy can further complicate treatment of non-tuberculous mycobacterial infections. we report a case of post liposuction mycobacterium abscessus surgical site infection in a returned medical tourist and occurrence of paradox ... | 2015 | 26753088 |
| high-throughput transcriptomics reveals common and strain-specific responses of human macrophages to infection with mycobacterium abscessus smooth and rough variants. | mycobacterium abscessus (mab) is an emerging pathogen causing pulmonary infections in those with inflammatory lung disorders, such as cystic fibrosis (cf), and is associated with the highest fatality rate among rapidly growing mycobacteria (rgm). phenotypically, mab manifests as either a smooth (mab-s) or a rough (mab-r) morphotype, which differ in their levels of cell wall glycopeptidolipids (gpls) and in their pathogenicity in vivo. as one of the primary immune cells encountered by mab, we sou ... | 2015 | 26654095 |
| amikacin pharmacokinetics/pharmacodynamics in a novel hollow-fiber mycobacterium abscessus disease model. | the treatment of pulmonary mycobacterium abscessus disease is associated with very high failure rates and easily acquired drug resistance. amikacin is the key drug in treatment regimens, but the optimal doses are unknown. no good preclinical model exists to perform formal pharmacokinetics/pharmacodynamics experiments to determine these optimal doses. we developed a hollow-fiber system model of m. abscessus disease and studied amikacin exposure effects and dose scheduling. we mimicked amikacin hu ... | 2015 | 26643339 |