TitleAbstractYear(sorted ascending)
effects of myxothiazol and 5-undecyl-6-hydroxy-4,7-dioxobenzothiazole on the respiratory pathways of the phytopathogenic fluorescent bacteria pseudomonas cichorii and pseudomonas aptata.myxothiazol inhibited the electron transport in the cytochrome b/c segment of membrane particles from pseudomonas cichorii. a residual nadh-oxidation due to the presence of an alternative pathway via cytochrome o (em, 7 = +250 mv) was sensitive to the quinone analog 5-undecyl-6-hydroxy-4,7-dioxobenzothiazole (uhdbt). this latter inhibitor was equally effective in blocking the linear respiratory chain of pseudomonas aptata, a strain deficient in cytochromes of c type and rieske iron-sulphur centr ...19854062483
effect of oxygen limitation on the formation of the electron transport system of the phytopathogenic fluorescent bacterium pseudomonas cichorii.the composition of the membrane-bound electron transport system of the phytopathogenic bacterium pseudomonas cichorii underwent modification in response to oxygen supply. growth adaptation to low oxygen concentrations was characterized by repression of cytochromes involved in ubiquinol-cyt. c oxidoreductase and cyt. c oxidase activities. by contrast, cyto. o, i.e., the alternative cyanide-insensitive oxidase of p. cichorii, was unaffected by low oxygen tension. no a-type cytochromes could be det ...19863025191
alpha-santonin 1,2-reductase and its role in the formation of dihydrosantonin and lumisantonin by pseudomonas cichorii s.1,2-dihydrosantonin is the first stable product in the degradative pathway of alpha-santonin by pseudomonas cichorii s. its formation is catalyzed by an oxidoreductase, which is nadh or nadph dependent and has an apparent km value of 66.66 microm for santonin and 44.33 microm for nadh. the enzyme activity is stable at ph 6.0, 7.0, and 8.0, and is not affected by edta and divalent metal ions. it is postulated that the enzymic reduction of santonin occurs via formation of a transient zwitterionic ...19873690421
mechanism of uptake of alpha-santonin by pseudomonas cichorii strain s. 198718581540
copper resistance gene homologs in pathogenic and saprophytic bacterial species from tomato.copper-resistant strains of xanthomonas campestris pv. vesicatoria, pseudomonas cichorii, pseudomonas putida, pseudomonas fluorescens, and a yellow pseudomonas sp. were isolated from tomato plants or seeds. in southern hybridizations, dna from each strain showed homology with the copper resistance (cop) operon previously cloned from pseudomonas syringae pv. tomato pt23. homology was associated with plasmid and chromosomal dna in x. compestris pv. vesicatoria, p. putida, and the yellow pseudomona ...199016348118
[the lipopolysaccharides of pseudomonas solanacearum i pseudomonas cichorii].structure analysis by the methods of methylation, 1h- and 13c-n. m. r. spectroscopy has shown that o-specific polysaccharides of typical strains of pseudomonas solanacearum (biovar i) and p. cichorii are identical by their structure and constructed of branched pentasaccharide repeating links which include three residues of rhamnose (one of them is in the branching node), one residue of beta-xylose (it occupies terminal position) and one reside of n-acetyl-beta-glucosamine. the other strain of p. ...19902273995
degradation of 1,3-dichloropropene by pseudomonas cichorii 170.the gram-negative bacterium pseudomonas cichorii 170, isolated from soil that was repeatedly treated with the nematocide 1, 3-dichloropropene, could utilize low concentrations of 1, 3-dichloropropene as a sole carbon and energy source. strain 170 was also able to grow on 3-chloroallyl alcohol, 3-chloroacrylic acid, and several 1-halo-n-alkanes. this organism produced at least three different dehalogenases: a hydrolytic haloalkane dehalogenase specific for haloalkanes and two 3-chloroacrylic acid ...19989687453
a simplified subtractive hybridization protocol used to isolate dna sequences specific to xylella fastidiosa.a simplified protocol of subtractive hybridization based on the technique of l. m. kunkel, a. p. monaco, w. middlesworth, h. d. ochs & s. a. latt (1985, proc natl acad sci usa, 82, 4778-4782) was used to obtain dna sequences specific to xylella fastidiosa isolated from diseased citrus plants. as a driver, dna extracted from bacteria showing different degrees of relatedness was used: xy. fastidiosa 788 isolated from another host (plum), xanthomonas campestris pv. campestris and burkholderia gladi ...199910463163
direct production of allitol from d-fructose by a coupling reaction using d-tagatose 3-epimerase, ribitol dehydrogenase and formate dehydrogenase.allitol was produced from d-fructose via a new nadh-regenerating enzymatic reaction system using d-tagatose 3-epimerase (d-te), ribitol dehydrogenase (rdh), and formate dehydrogenase (fdh). d-fructose was epimerized to d-psicose by the d-te of pseudomonas cichorii st-24 and the d-psicose was subsequently reduced to allitol by the rdh of an rdh-constitutive mutant, x-22, derived from klebsiella pneumoniae ifo 3321. nadh regeneration for the reduction of d-psicose by the rdh was achieved by the ir ...200016232907
production of antimicrobial defensin in nicotiana benthamiana with a potato virus x vector.a recombinant plasmid,, was constructed to express the gene-encoding wasabi (wasabia japonica) defensin with the potato virus x (pvx) vector. allows the expression of defensin in the host nicotiana benthamiana, and the defensin protein wt1 can be purified from virus-infected leaves by heat treatment and affinity chromatography. wt1 exhibits strong antifungal activity toward the phytopathogenic fungi magnaporthe grisea (50% inhibitory concentration [ic50] = 5 microg/ml) and botryt ...200111204773
direct biosynthesis of poly(3-hydroxyalkanoates) bearing epoxide groups.the biosynthesis of poly(3-hydroxyalkanoates) (phas) by pseudomonas cichorii yn2 cultured with c6-c12 1-alkenes was studied. phas containing repeating units with terminal epoxide groups were obtained when c7-c12 1-alkenes were fed separately as the only carbon source, but no epoxidized unit was detected when 1-hexene was fed. the content of epoxidized units in the polymers was in the range of 4-20 mol%, which was not dependent on the c atom length of the 1-alkene used as a substrate. the polymer ...200111718827
a novel plant defensin-like gene of winter wheat is specifically induced during cold acclimation.a novel cdna clone, tad1, was isolated from crown tissue of winter wheat after differential screening of cold acclimation-induced genes. the tad1 cdna encoded a 23kda polypeptide with a potential n-terminal signal sequence. the putative mature sequence showed striking similarity to plant defensins or gamma-thionins, representing low molecular size antipathogenic polypeptides. high levels of tad1 mrna accumulation occurred within one day of cold acclimation in crown tissue and the level was maint ...200212379218
fluorescent pseudomonas species causing post-harvest decay of endives in argentina.a post-harvest bacterial decay was observed on ready-to-use french endives in argentina. affected chicons showed browning and soft-rot of inner leaves and marginal necrosis. physiological and biochemical tests allowed us to identify the isolates from endive as pseudomonas fluorescens bv. iii, pseudomonas fluorescens bv. v, and pseudomonas cichorii. pathogenicity was verified on rtu healthy endives by inoculation with each bacterial species, and also with the mixture of the 3 strains. p. cichorii ...200212600002
virus-induced silencing of wipk and sipk genes reduces resistance to a bacterial pathogen, but has no effect on the inf1-induced hypersensitive response (hr) in nicotiana benthamiana.activation of two mitogen-activated protein kinases (mapks), wound-induced protein kinase (wipk) and salicylic acid-induced protein kinase (sipk), is one of the earliest responses that occur in tobacco plants that have been wounded, treated with pathogen-derived elicitors or challenged with avirulent pathogens. we isolated cdnas for these mapks (nbwipkand nbsipk) from nicotiana benthamiana. the function of nbwipk and nbsipk in mediating the hypersensitive response (hr) triggered by infiltration ...200312838412
high-performance liquid chromatography analyses of pyoverdin siderophores differentiate among phytopathogenic fluorescent pseudomonas species.the relationship of pyoverdins produced by 41 pathovars of pseudomonas syringae and by phytopathogenic pseudomonas species was investigated. a high-performance liquid chromatography method for analyzing the culture medium proved to be superior to isoelectric focusing for detecting pyoverdin production, for differentiating slightly different pyoverdins, and for differentiating atypical from typical fe(iii)-chelated pyoverdins. nonfluorescent strains were found in pseudomonas amygdali, pseudomonas ...200312571041
cytosolic hsp90 and hsp70 are essential components of inf1-mediated hypersensitive response and non-host resistance to pseudomonas cichorii in nicotiana benthamiana.summary mitogen-activated protein kinases (mapks) play pivotal roles in the signal transduction pathway of plant defence responses against pathogens. a search for mapk-interacting proteins revealed an interaction between a nicotiana benthamiana mapk, sipk (nbsipk) and cytosolic hsp90 (nbhsp90c-1) in yeast two-hybrid assay. to study the function of hsp90 in disease resistance, we silenced nbhsp90c-1 in n. benthamiana by virus-induced gene silencing (vigs) with potato virus x (pvx). nbhsp90c-1 sil ...200320569398
mutagenic dna repair potential in pseudomonas spp., and characterization of the rulabpc operon from the highly mutable strain pseudomonas cichorii 302959.we assessed the tolerance to ultraviolet b (uvb; 290-320 nm) radiation and uvb-induced mutability in 28 pseudomonas spp. and four burkholderia cepacia strains. the uvb survival of 23 (72%) of the strains was elevated (>46% survival following irradiation with a 2250 j m-2 dose), and 17 (53%) strains were defined as mutable by uvb. a mutagenic dna repair determinant was cloned and characterized from the highly mutable strain p. cichorii 302959 and shown by sequence analysis to be an allele of rula ...200415052319
the pyoverdins of pseudomonas syringae and pseudomonas cichorii.the structure elucidation of the cyclic (lactonic) forms of the pyoverdins with a succinamide side chain originally produced by the closely related species pseudomonas syringae and p. cichorii is reported. mass spectrometry and nuclear magnetic resonance analyses as well as the determination of the configuration of the amino acids after degradation indicate that these two pyoverdins differ only by the replacement of the first in-chain serine by glycine. the pyoverdins of p. syringae and p. cicho ...200415540590
a novel enzymatic approach to the massproduction of l-galactose from l-sorbose.wild-type strain of pseudomonas cichorii st-24 was unable to grow on d -psicose and inductively produced d -tagatose 3-epimerase (d -te) with d -tagatose as an inducer. we have isolated a constitutive mutant, designated strain ka75, which had acquired a new ability to grow on a mineral salts medium containing d -psicose as a sole carbon source. the d -psicose-metabolizing mutant synthesized a high level of d -te. when grown on the culture medium supplemented with mn(2+), the mutant strain produc ...200416233647
high-throughput in planta expression screening identifies a class ii ethylene-responsive element binding factor-like protein that regulates plant cell death and non-host resistance.we performed high-throughput screening using the potato virus x (pvx) system to overexpress nicotiana benthamiana genes in planta and identify positive regulators of cell death. this screening identified nbcd1, a novel class ii ethylene-responsive element binding factor (erf), as a potent inducer of the hypersensitive response (hr)-like cell death. nbcd1 expression was induced by treatments with inf1 elicitor and a non-host pathogen pseudomonas cichorii. nbcd1 exhibited transcriptional repressor ...200516098104
induction of apoptotic cell death leads to the development of bacterial rot caused by pseudomonas cichorii.pseudomonas cichorii is the major causal agent of bacterial rot of lettuce. collapse and browning symptoms were observed in lettuce leaf tissue from 15 to 24 h after inoculation (hai) with p. cichorii; superoxide anion generation was detected at 1 to 6 hai; and cell death was induced at 6 hai, reaching a maximum at approximately 9 and 12 hai. heterochromatin condensation and dna laddering also were observed within 3 hai. pharmacological studies showed that induction of cell death and dna ladderi ...200616529373
comparative analysis of induction pattern of programmed cell death and defense-related responses during hypersensitive cell death and development of bacterial necrotic leaf spots in eggplant.pseudomonas cichorii causes necrotic leaf spots (nls), while pseudomonas syringae pv. tabaci induces a hypersensitive response (hr) in eggplant. p. cichorii induced cell death at 9 h after inoculation (hai), reaching a maximum of around 24-30 hai. on the other hand, cell death was induced 6 hai with p. syringae pv. tabaci, reaching a maximum of around 12-18 hai. superoxide generation was observed in eggplant inoculated with both bacteria. dna fragmentation, cytochrome c release into the cytosol ...200616614819
purification, crystallization and preliminary x-ray diffraction studies of d-tagatose 3-epimerase from pseudomonas cichorii.d-tagatose 3-epimerase (d-te) from pseudomonas cichorii catalyzes the epimerization of various ketohexoses at the c3 position. the epimerization of d-psicose has not been reported with epimerases other than p. cichorii d-te and d-psicose 3-epimerase from agrobacterium tumefaciens. recombinant p. cichorii d-te has been purified and crystallized. crystals of p. cichorii d-te were obtained by the sitting-drop method at room temperature. the crystal belongs to the monoclinic space group p2(1), with ...200717277456
a high-throughput screen of cell-death-inducing factors in nicotiana benthamiana identifies a novel mapkk that mediates inf1-induced cell death signaling and non-host resistance to pseudomonas cichorii.a high-throughput overexpression screen of nicotiana benthamiana cdnas identified a gene for a mitogen-activated protein kinase kinase (mapkk) as a potent inducer of the hypersensitive response (hr)-like cell death. nbmkk1 protein is localized to the nucleus, and the n-terminal putative mapk docking site of nbmkk1 is required for its function as a cell-death inducer. nbmkk1-mediated leaf-cell death was compromised in leaves where nbsipk expression was silenced by virus-induced gene silencing. a ...200717319846
crystal structures of d-tagatose 3-epimerase from pseudomonas cichorii and its complexes with d-tagatose and d-fructose.pseudomonas cichoriiid-tagatose 3-epimerase (p. cichoriid-te) can efficiently catalyze the epimerization of not only d-tagatose to d-sorbose, but also d-fructose to d-psicose, and is used for the production of d-psicose from d-fructose. the crystal structures of p. cichoriid-te alone and in complexes with d-tagatose and d-fructose were determined at resolutions of 1.79, 2.28, and 2.06 a, respectively. a subunit of p. cichoriid-te adopts a (beta/alpha)(8) barrel structure, and a metal ion (mn(2+) ...200717936787
a novel mapkk involved in cell death and defense signaling.a high-throughput in planta overexpression screen of a nicotiana benthamiana cdna library identified a mitogen activated protein kinase kinase (mapkk), nbmkk1, as a potent inducer of hypersensitive response (hr)-like cell death. nbmkk1-mediated cell death was attenuated in plants whereby expression of nbsipk, an ortholog of tobacco sipk and arabidopsis atmpk6, was knocked down by virus-induced gene silencing (vigs), suggesting that nbmkk1 functions upstream of nbsipk. in accordance with this res ...200719704612
high-throughput in planta expression screening identifies an adp-ribosylation factor (arf1) involved in non-host resistance and r gene-mediated identify positive regulators of cell death in plants, we performed a high-throughput screening, employing potato virus x-based overexpression in planta of a cdna library derived from paraquat-treated nicotiana benthamiana leaves. the screening of 30,000 cdna clones enabled the identification of an adp-ribosylation factor 1 (arf1) that induces cell death when overexpressed in n. benthamiana. overexpression of the guanosine diphosphate (gdp)-locked mutant of arf1 did not trigger cell death, sug ...200818705881
ntbzip60, an endoplasmic reticulum-localized transcription factor, plays a role in the defense response against bacterial pathogens in nicotiana tabacum.a spermine-based signal transduction pathway plays a defensive role against incompatible pathogens. we identified a novel spermine-responsive cdna from nicotiana tabacum that encodes a basic region/leucine zipper protein with a putative transmembrane domain. identity to arabidopsis thaliana atbzip60 was sufficiently high to name the novel cdna ntbzip60. expression analysis revealed that ntbzip60 is a component of the spermine-signal pathway, and is also involved in the unfolded protein response ...200818758894
the hrp genes of pseudomonas cichorii are essential for pathogenicity on eggplant but not on lettuce.pseudomonas cichorii causes necrotic lesions in eggplant and rot in lettuce. through transposon insertion into p. cichorii strain spc9018 we produced two mutants, 4-57 and 2-99, that lost virulence on eggplant but not lettuce. analyses showed that a transposon was inserted into the hrpg gene in 4-57 and the hrct gene in 2-99. nucleotide sequences of the hrp genes of spc9018 are homologous to those of pseudomonas viridiflava bs group strains. the pathogenicity of 4-57 on eggplant was restored by ...200818832299
comparative expression analysis of genes induced during development of bacterial rot and induction of hypersensitive cell death in lettuce.the development of bacterial rot disease caused by pseudomonas cichorii is closely associated with programmed cell death. to investigate the molecular events occurring during the development of bacterial rot, we isolated 20 p. cichorii-responsive genes (pcrgs) in lettuce by differential display. among these pcrgs, signal transduction-, transcription/translation- and defense/stress responses-related pcrgs were subjected to a comparative expression study. we used rna samples isolated from lettuce ...200818171591
plant voltage-dependent anion channels are involved in host defense against pseudomonas cichorii and in bax-induced cell death.the voltage-dependent anion channel (vdac) is a major outer mitochondrial membrane protein. it is well documented that vdac plays an important role in apoptosis, a kind of programmed cell death, in mammalian systems. however, little is known about the role of the plant counterpart during the process of plant-specific cell death such as pathogen-induced hypersensitive response. to address this issue, we isolated three vdac full-length cdnas (ntvdac1-3) from nicotiana tabacum. the deduced products ...200918953543
long-term effects of inducible mutagenic dna repair on relative fitness and phenotypic diversification in pseudomonas cichorii 302959.mutagenic dna repair (mdr) employs low-fidelity dna polymerases capable of replicating past dna lesions resulting from exposure to high-energy ultraviolet radiation (uvr). mdr confers uvr tolerance and activation initiates a transient mutator phenotype that may provide opportunities for adaptation. to investigate the potential role of mdr in adaptation, we have propagated parallel lineages of the highly mutable epiphytic plant pathogen pseudomonas cichorii 302959 with daily uvr activation (uvr l ...200918984570
serine palmitoyltransferase, the first step enzyme in sphingolipid biosynthesis, is involved in nonhost overexpression screen of nicotiana benthamiana cdnas identified a gene for the lcb2 subunit of serine palmitoyltransferase (spt) as a potent inducer of hypersensitive response-like cell death. the pyridoxal 5'-phosphate binding site of nblcb2 is required for its function as a cell death inducer. nblcb2 mrna is accumulated after infection by nonhost pathogen pseudomonas cichorii. resistance of n. benthamiana against p. cichorii was compromised by treatment with an spt inhibitor and in nblcb2- ...200919061400
pseudomonas cichorii as the causal agent of midrib rot, an emerging disease of greenhouse-grown butterhead lettuce in flanders.bacterial midrib rot of greenhouse-grown butterhead lettuce (lactuca sativa l. var. capitata) is an emerging disease in flanders (belgium) and fluorescent pseudomonads are suspected to play an important role in the disease. isolations from infected lettuces, collected from 14 commercial greenhouses in flanders, yielded 149 isolates that were characterized polyphasically, which included morphological characteristics, pigmentation, pathogenicity tests by both injection and spraying of lettuce, lop ...200919157742
polyamines as a common source of hydrogen peroxide in host- and nonhost hypersensitive response during pathogen infection.the hypersensitive response (hr) is a powerful resistance system that plants have developed against pathogen attack. there are two major pathways for hr induction; one is through recognition of the pathogen by a specific host protein, and is known as the host hr. the other is through common biochemical changes upon infection--the nonhost hr. we previously demonstrated that hydrogen peroxide derived from polyamine degradation by polyamine oxidase triggers the typical host hr in tobacco plants upo ...200919190986
structure of a d-tagatose 3-epimerase-related protein from the hyperthermophilic bacterium thermotoga maritima.the crystal structure of a d-tagatose 3-epimerase-related protein (tm0416p) encoded by the hypothetical open reading frame tm0416 in the genome of the hyperthermophilic bacterium thermotoga maritima was determined at a resolution of 2.2 a. the asymmetric unit contained two homologous subunits and a dimer was generated by twofold symmetry. the main-chain coordinates of the enzyme monomer proved to be similar to those of d-tagatose 3-epimerase from pseudomonas cichorii and d-psicose 3-epimerase fr ...200919255464
initial in vitro evaluations of the antibacterial activities of glucosinolate enzymatic hydrolysis products against plant pathogenic bacteria.the aim of the study was to evaluate the in vitro antibacterial effects of glucosinolate hydrolysis products (ghp) against plant pathogenic micro-organisms namely agrobacterium tumefaciens, erwinia chrysanthemi, pseudomonas cichorii, pseudomonas tomato, xanthomonas campestris and xanthomonas juglandis.200919291239
s-glycoprotein-like protein regulates defense responses in nicotiana plants against ralstonia solanacearum.rsrga4 (for ralstonia solanacearum-responsive gene a4) encodes a polypeptide similar to s-locus glycoprotein (sgp) from brassica rapa and sgp-like proteins from ipomoea trifida and medicago truncatula. therefore, we designated rsrga4 as ntsglp (for nicotiana tabacum sgp-like protein) and nbsglp (its nicotiana benthamiana ortholog). nbsglp is expressed in root, leaf, petal, gynoecium, and stamen. expression of nbsglp was strongly induced by inoculation with an avirulent strain of r. solanacearum ...201020118275
growth parameter components of adaptive specificity during experimental evolution of the uvr-inducible mutator pseudomonas cichorii 302959.mutagenic dna repair (mdr) transiently increases mutation rate through the activation of low-fidelity repair polymerases in response to specific, dna-damaging environmental stress conditions such as ultraviolet radiation (uvr) exposure. these repair polymerases also confer uvr tolerance, intimately linking mutability and survival in bacteria that colone habitats subject to regular uvr exposure.201121264242
characterization and expression of attacin, an antibacterial protein-encoding gene, from the beet armyworm, spodoptera exigua (hübner) (insecta: lepidoptera: noctuidae).to isolate antimicrobial-related genes from the beet armyworm, spodoptera exigua, we performed genefishing, a polymerase chain reaction (pcr)-based differential display technique. an attacin-like complementary dna (cdna) including a 3'-untranslated region was identified from among 18 over-expressed genes in microbial-infected larvae. the full-length attacin cdna from s. exigua cdna (seattacin) was cloned using rapid amplification of cdna ends pcr. the attacin-like cdna transcript was 765 nucleot ...201122160467
implication of an aldehyde dehydrogenase gene and a phosphinothricin n-acetyltransferase gene in the diversity of pseudomonas cichorii virulence.pseudomonas cichorii harbors the hrp genes. hrp-mutants lose their virulence on eggplant but not on lettuce. a phosphinothricin n-acetyltransferase gene (pat) is located between hrpl and an aldehyde dehydrogenase gene (aldh) in the genome of p. cichorii. comparison of nucleotide sequences and composition of the genes among pseudomonads suggests a common ancestor of hrp and pat between p. cichorii strains and p. viridiflava strains harboring the single hrp pathogenicity island. in contrast, phylo ...201124704843
genetic diversity and ecological evaluation of fluorescent pseudomonads isolated from the leaves and roots of potato plants.a total of 828 isolates of fluorescent pseudomonads (fps) were obtained from the leaves (305 isolates) and roots (523 isolates) of potato plants grown in different geographical locations in japan, and 16s rrna gene sequences of 776 isolates were successfully determined by direct pcr sequencing. clustering analysis (≥99% identity) identified 13 and 26 operational taxonomic units (otus) for leaf- and root-associated fps, respectively, and 29 otus were identified in the phytosphere of potato plants ...201222791043
a translationally controlled tumor protein negatively regulates the hypersensitive response in nicotiana benthamiana.we have been isolating and characterizing ralstonia solanacearum-responsive genes (rsrgs) in nicotiana plants. in this study we focused on rsrg308, which we renamed nbtctp (n. benthamiana translationally controlled tumor protein) because it encodes a polypeptide showing similarity to translationally controlled tumor proteins. induction of the hypersensitive response (hr) was accelerated in nbtctp-silenced n. benthamiana plants challenged with r. solanacearum 8107 (rs8107). the rs8107 population ...201323788648
new linear lipopeptides produced by pseudomonas cichorii sf1-54 are involved in virulence, swarming motility, and biofilm formation.pseudomonas cichorii is the causal agent of lettuce midrib rot, characterized by a dark-brown to green-black discoloration of the midrib. formation of necrotic lesions by several plant-pathogenic pseudomonads is associated with production of phytotoxic lipopeptides, which contribute to virulence. therefore, the ability of p. cichorii sf1-54 to produce lipopeptides was investigated. a cell-free culture filtrate of sf1-54 showed surfactant, antimicrobial, and phytotoxic activities which are typica ...201323405865
structural insight into l-ribulose 3-epimerase from mesorhizobium loti.l-ribulose 3-epimerase (l-re) from mesorhizobium loti has been identified as the first ketose 3-epimerase that shows the highest observed activity towards ketopentoses. in the present study, the crystal structure of the enzyme was determined to 2.7 å resolution. the asymmetric unit contained two homotetramers with the monomer folded into an (α/β)8-barrel carrying four additional short α-helices. the overall structure of m. loti l-re showed significant similarity to the structures of ketose 3-epi ...201324311575
an effector gene hopa1 influences on virulence, host specificity, and lifestyles of pseudomonas cichorii jbc1.pseudomonas cichorii is a devastating pathogen which infects a wide range of ornamental as well as agricultural crops worldwide. characterization of virulence genes helps to understand pathogens' infection processes, which may lead to development of resistant crops. for functional validation of novel genes, we re-constructed pucp18 vector with λ phage red operon and sacb gene (pucp18_reds), which simplified conventional marker exchange system. the effector gene hopa1 of p. cichorii jbc1 was mark ...201425127676
structure of d-tagatose 3-epimerase-like protein from methanocaldococcus jannaschii.the crystal structure of a d-tagatose 3-epimerase-like protein (mj1311p) encoded by a hypothetical open reading frame, mj1311, in the genome of the hyperthermophilic archaeon methanocaldococcus jannaschii was determined at a resolution of 2.64 å. the asymmetric unit contained two homologous subunits, and the dimer was generated by twofold symmetry. the overall fold of the subunit proved to be similar to those of the d-tagatose 3-epimerase from pseudomonas cichorii and the d-psicose 3-epimerases ...201425005083
efficacy of lactoferricin b in controlling ready-to-eat vegetable spoilage caused by pseudomonas spp.the microbial content of plant tissues has been reported to cause the spoilage of ca. 30% of chlorine-disinfected fresh vegetables during cold storage. the aim of this work was to evaluate the efficacy of antimicrobial peptides in controlling microbial vegetable spoilage under cold storage conditions. a total of 48 bacterial isolates were collected from ready-to-eat (rte) vegetables and identified as belonging to acinetobacter calcoaceticus, aeromonas media, pseudomonas cichorii, pseudomonas flu ...201526453993
cell death-inducing stresses are required for defense activation in ds1-phosphatidic acid phosphatase-silenced nicotiana benthamiana.we previously identified ds1 plants that showed resistance to compatible ralstonia solanacearum with accelerated defense responses. here, we describe activation mechanisms of defense responses in ds1 plants. after inoculation with incompatible r. solanacearum 8107, ds1 plants showed hyperinduction of hypersensitive response (hr) and reactive oxygen species (ros) generation. transient expression of popp1 and avra induced hyperinduction of hr and ros generation. furthermore, pseudomonas cichorii ( ...201526188395
characterization of cichopeptins, new phytotoxic cyclic lipodepsipeptides produced by pseudomonas cichorii sf1-54 and their role in bacterial midrib rot disease of lettuce.the lettuce midrib rot pathogen pseudomonas cichorii sf1-54 produces seven bioactive compounds with biosurfactant properties. two compounds exhibited necrosis-inducing activity on chicory leaves. the structure of the two phytotoxic compounds, named cichopeptin a and b, was tentatively characterized. they are related cyclic lipopeptides composed of an unsaturated c12-fatty acid chain linked to the n-terminus of a 22-amino acid peptide moiety. cichopeptin b differs from cichopeptin a only in the l ...201525961750
green and red light reduces the disease severity by pseudomonas cichorii jbc1 in tomato plants via upregulation of defense-related gene expression.light influences many physiological processes in most organisms. to investigate the influence of light on plant and pathogen interaction, we challenged tomato seedlings with pseudomonas cichorii jbc1 by flood inoculation and incubated the seedlings under different light conditions. tomato seedlings exposed to green or red light showed a significant reduction in disease incidence compared with those grown under white light or dark conditions. to understand the underlying mechanisms, we investigat ...201525536016
novel components of leaf bacterial communities of field-grown tomato plants and their potential for plant growth promotion and biocontrol of tomato diseases.this work aimed to characterize potentially endophytic culturable bacteria from leaves of cultivated tomato and analyze their potential for growth promotion and biocontrol of diseases caused by botrytis cinerea and pseudomonas syringae. bacteria were obtained from inner tissues of surface-disinfected tomato leaves of field-grown plants. analysis of 16s rrna gene sequences identified bacterial isolates related to exiguobacterium aurantiacum (isolates bt3 and mt8), exiguobacterium spp. (isolate gt ...201626654914
x-ray structures of the pseudomonas cichorii d-tagatose 3-epimerase mutant form c66s recognizing deoxy sugars as substrates.pseudomonas cichorii d-tagatose 3-epimerase (pcdte), which has a broad substrate specificity, efficiently catalyzes the epimerization of not only d-tagatose to d-sorbose but also d-fructose to d-psicose (d-allulose) and also recognizes the deoxy sugars as substrates. in an attempt to elucidate the substrate recognition and catalytic reaction mechanisms of pcdte for deoxy sugars, the x-ray structures of the pcdte mutant form with the replacement of cys66 by ser (pcdte_c66s) in complexes with deox ...201627368739
elucidation of the functional role of flagella in virulence and ecological traits of pseudomonas cichorii using flagella absence (δflij) and deficiency (δflii) mutants.understanding the infection mechanisms of pathogens will lead to better management of the associated diseases. the flagella of these pathogens play significant roles not only in bacterial motility, but also in virulence. in the present study, two genes involved in flagella construction, flij and flii of pseudomonas cichorii, were analyzed. the results revealed that these genes are vital for flagella formation and play significant roles not only in motility, but also in virulence. when we inocula ...201626829306
visual analysis for detection and quantification of pseudomonas cichorii disease severity in tomato plants.pathogen infection in plants induces complex responses ranging from gene expression to metabolic processes in infected plants. in spite of many studies on biotic stress-related changes in host plants, little is known about the metabolic and phenotypic responses of the host plants to pseudomonas cichorii infection based on image-based analysis. to investigate alterations in tomato plants according to disease severity, we inoculated plants with different cell densities of p. cichorii using dipping ...201627493605
genome-wide analyses of chitin synthases identify horizontal gene transfers towards bacteria and allow a robust and unifying classification into fungi.chitin, the second most abundant biopolymer on earth after cellulose, is found in probably all fungi, many animals (mainly invertebrates), several protists and a few algae, playing an essential role in the development of many of them. this polysaccharide is produced by type 2 glycosyltransferases, called chitin synthases (chs). there are several contradictory classifications of chs isoenzymes and, as regards their evolutionary history, their origin and diversity is still a matter of debate.201627881071
bacterial characterization in ambient submicron particles during severe haze episodes at ji'nan, january 2014, severe haze episodes which sweep across chinese cities have attracted public concern and interest at home and abroad. in addition to the physicochemical properties of air pollutants, bacteria are thought to be responsible for the spread of respiratory diseases and various allergies. we attempted the bacterial characterization of submicron particles (pm0.18-0.32, pm0.32-0.56, and pm0.56-1) under severe haze episodes using high-throughput sequencing and real-time quantitative pcr ...201728017418
chemical structure of the lipid a component of pseudomonas sp. strain pamc 28618 from thawing permafrost in relation to pathogenicity.climate change causes permafrost thawing, and we are confronted with the unpredictable risk of newly discovered permafrost microbes that have disease-causing capabilities. here, we first characterized the detailed chemical structure of the lipid a moiety from a pseudomonas species that was isolated from thawing arctic permafrost using maldi-based mass spectrometric approaches (i.e., maldi-tof ms and maldi-qit-tof ms(n)). the maldi multi-stage mass spectrometry (ms) analysis of lipid a extracted ...201728526845
a novel phylogroup of pseudomonas cichorii identified following an unusual disease outbreak on tomato.recently, in central florida tomato production fields, tomato foliage and fruit were observed with symptoms similar to bacterial speck. fluorescent pseudomonads were consistently isolated and the strains were characterized by standard lopat tests, pathogenicity tests, and genetic characterization using 16s ribosomal rna (rrna) sequences and multilocus sequence analysis (mlsa) of conserved housekeeping genes. lopat test results indicated that the strains were likely pseudomonas cichorii. these st ...201728613108
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