| antifungal and cytotoxic assessment of lapachol derivatives produced by fungal biotransformation. | in the screening for biological active compounds, the biotransformation processes catalyzed by filamentous fungi are useful because they can provide information about the possible appearance of toxic metabolites after oral administration and also generate new leads. in this paper, biotransformation of lapachol (1) by three fungal strains, mucor circinelloides nrrl3631, botrytis cinerea uca992 and botrytis cinerea 2100, has been investigated for the first time. lapachol (1) was biotransformed int ... | 2016 | 26996030 |
| rnai function, diversity, and loss in the fungal kingdom. | rnai is conserved and has been studied in a broad cross-section of the fungal kingdom, including neurospora crassa, schizosaccharomyces pombe, cryptococcus neoformans, and mucor circinelloides. and yet well known species, including the model yeast saccharomyces cerevisiae and the plant pathogen ustilago maydis, have lost rnai, providing insights and opportunities to illuminate benefits conferred both by the presence of rnai and its loss. some of the earliest studies of rnai were conducted in neu ... | 2013 | 24173579 |
| the interaction of fungi with the environment orchestrated by rnai. | the fungal kingdom has been key in the investigation of the biogenesis and function of small rnas (srnas). the discovery of phenomena such as quelling in neurospora crassa represents pioneering work in the identification of the main elements of the rna interference (rnai) machinery. recent discoveries in the regulatory mechanisms in some yeast and filamentous fungi are helping us reach a deeper understanding of the transcriptional and post-transcriptional gene-silencing mechanisms involved in ge ... | 2016 | 26932186 |
| the calcineurin pathway inhibitor tacrolimus enhances the in vitro activity of azoles against mucorales via apoptosis. | the calcineurin pathway regulates antifungal drug resistance and the virulence of several major human-pathogenic fungi, including the recalcitrant mucorales. we hypothesized that the fungistatic triazoles posaconazole (pcz) and itraconazole (icz) become fungicidal in the setting of the calcineurin inhibitor tacrolimus (tcr) and that such an effect is mediated through apoptosis. fungicidal activity and apoptosis were studied using standard microbiological techniques and hyphal metabolic and vital ... | 2013 | 23851337 |
| [characterization of a malic enzyme isoform v from mucor circinelloides]. | we aimed at characterizing a malic enzyme isoform v from mucor circinelloides. | 2016 | 27373079 |
| a new regulatory mechanism controlling carotenogenesis in the fungus mucor circinelloides as a target to generate β-carotene over-producing strains by genetic engineering. | carotenoids are natural pigments with antioxidant properties that have important functions in human physiology and must be supplied through the diet. they also have important industrial applications as food colourants, animal feed additives and nutraceuticals. some of them, such as β-carotene, are produced on an industrial scale with the use of microorganisms, including fungi. the mucoral blakeslea trispora is used by the industry to produce β-carotene, although optimisation of production by mol ... | 2016 | 27266994 |
| increased virulence of cunninghamella bertholletiae in experimental pulmonary mucormycosis: correlation with circulating molecular biomarkers, sporangiospore germination and hyphal metabolism. | members of the order mucorales are emerging invasive molds that cause infections in immunocompromised patients. however, little is known about the relation between different species of mucorales and their virulence in invasive pulmonary mucormycosis. based upon our earlier epidemiological studies, we hypothesized that cunninghamella bertholletiae would demonstrate increased virulence. therefore, we studied the relative virulence of c. bertholletiae (cb), rhizopus oryzae (ro), r. microsporus (rm) ... | 2013 | 22686246 |
| effect of the filamentous fungus mucor circinelloides on the development of eggs of the rumen fluke calicophoron daubneyi (paramphistomidae). | ruminants infected by paramphistomidae flukes shed eggs in the feces, which pass through different stages in the environment until the infective stages (metacercariae) are reached. the activity of the soil fungus mucor circinelloides on the development of eggs of the rumen fluke calicophoron daubneyi was presently tested with 3 probes, i.e., in petri plates, feces and an aqueous environment (tubes). the effect of the fungus was assessed by recording the numbers of undeveloped, non-viable and emb ... | 2017 | 28165868 |
| a white collar 1-like protein mediates opposite regulatory functions in mucor circinelloides. | protein ubiquitylation plays a major role in the regulation of many cellular processes by altering the stability, localization or function of target proteins. crga is a protein of mucor circinelloides that shows the characteristics of ubiquitin ligases and is involved in the regulation of carotenogenesis and asexual sporulation in this fungus. crga, which belongs to a poorly characterized group of proteins present in almost all eukaryotes, represses carotenogenesis through the proteolysis-indepe ... | 2013 | 23357353 |
| identification and characterization of d-xylulokinase from the d-xylose-fermenting fungus, mucor circinelloides. | d-xylulokinase catalyzes the phosphorylation of d-xylulose in the final step of the pentose catabolic pathway to form d-xylulose-5-phosphate. the d-xylulokinase activity was found to be induced by both d-xylose and l-arabinose, as well as some of the other enzymes involved in the pentose catabolism, in the d-xylose-fermenting zygomycetous fungus, mucor circinelloides nbrc 4572. the putative gene, xyl3, which may encode d-xylulokinase, was detected in the genome sequence of this strain. the amino ... | 2014 | 25163569 |
| mixed production of filamentous fungal spores for preventing soil-transmitted helminth zoonoses: a preliminary analysis. | helminth zoonoses are parasitic infections shared by humans and animals, being the soil-transmitted helminths (sths) mainly caused by roundworms (ascarids) and hookworms. this study was aimed to assess the individual and/or mixed production of two helminth-antagonistic fungi, one ovicide (mucor circinelloides) and other predator (duddingtonia flagrans). fungi were grown both in petri plates and in a submerged culture (composed by water, nacl, na2hpo4 · 12 h2o, and wheat (triticum aestivum)). a f ... | 2013 | 23710451 |
| isolation of fungal pathogens to an edible mushroom, pleurotus eryngii, and development of specific its primers. | fungal pathogens have caused severe damage to the commercial production of pleurotus eryngii, the king oyster mushroom, by reducing production yield, causing deterioration of commercial value, and shortening shelf-life. four strains of pathogenic fungi, including trichoderma koningiopsis dc3, phomopsis sp. mp4, mucor circinelloides mp5, and cladosporium bruhnei mp6, were isolated from the bottle culture of diseased p. eryngii. a species-specific primer set was designed for each fungus from the i ... | 2013 | 24493949 |
| quantitative multiplexed detection of common pulmonary fungal pathogens by labeled primer polymerase chain reaction. | invasive fungal infections are an important cause of morbidity and mortality among immunocompromised patients. | 2014 | 25357108 |
| effects of 7-hydroxycalamenene isolated from croton cajucara essential oil on growth, lipid content and ultrastructural aspects of rhizopus oryzae. | the leaves and bark of croton cajucara, a shrub from the amazon region, have been used in folk medicine to treat diabetes, malaria, and gastrointestinal and liver disorders. the essential oil from the leaves, rich in linalool, presented antileishmanial and antimicrobial activities. a chemotype of this species was found with an essential oil rich in 7-hydroxycalamenene. during our studies of the c. cajucara essential oil, we isolated 7-hydroxycalamenene at > 98 % purity. the minimum inhibitory co ... | 2014 | 24841967 |
| genetic basis of carotenoid overproduction in fusarium oxysporum. | the phytopathogenic fungus fusarium oxysporum is a model organism in the study of plant-fungus interactions. as other fusarium species, illuminated cultures of f. oxysporum exhibit an orange pigmentation because of the synthesis of carotenoids, and its genome contains orthologous light-regulated car genes for this biosynthetic pathway. by chemical mutagenesis, we obtained carotenoid overproducing mutants of f. oxysporum, called cars, with upregulated mrna levels of the car genes. to identify the ... | 2012 | 22750191 |
| in vitro antifungal susceptibility of filamentous fungi causing rare infections: synergy testing of amphotericin b, posaconazole and anidulafungin in pairs. | mucormycetes (formerly known as zygomycetes of the order mucorales) and hyaline moulds such as those of the genus fusarium or paecilomyces are emerging as significant human pathogens. the aim of the study was to determine the in vitro antifungal susceptibility of these fungi to older and newer antifungals and to investigate the antifungal activity of amphotericin b, posaconazole and anidulafungin in dual combinations. | 2012 | 22535624 |
| in vitro activity of isavuconazole and comparators against clinical isolates of the mucorales order. | the in vitro activity of isavuconazole against mucorales isolates measured by eucast e.def 9.2 and clsi m38-a2 methodologies was investigated in comparison with those of amphotericin b, posaconazole, and voriconazole. seventy-two isolates were included: 12 of lichtheimia corymbifera, 5 of lichtheimia ramosa, 5 of group i and 9 of group ii of mucor circinelloides, 9 of rhizomucor pusillus, 26 of rhizopus microsporus, and 6 of rhizopus oryzae. species identification was confirmed by internal trans ... | 2015 | 26438494 |
| (13)c-metabolic flux analysis of lipid accumulation in the oleaginous fungus mucor circinelloides. | the oleaginous fungus mucor circinelloides is of industrial interest because it can produce high levels of polyunsaturated fatty acid γ-linolenic acid. m. circinelloides cbs 277.49 is able to accumulate less than 15% of cell dry weight as lipids, while m. circinelloides wj11 can accumulate lipid up to 36%. in order to better understand the mechanisms behind the differential lipid accumulation in these two strains, tracer experiments with (13)c-glucose were performed with the growth of m. circine ... | 2015 | 26318243 |
| a non-canonical rna degradation pathway suppresses rnai-dependent epimutations in the human fungal pathogen mucor circinelloides. | mucorales are a group of basal fungi that includes the casual agents of the human emerging disease mucormycosis. recent studies revealed that these pathogens activate an rnai-based pathway to rapidly generate drug-resistant epimutant strains when exposed to stressful compounds such as the antifungal drug fk506. to elucidate the molecular mechanism of this epimutation pathway, we performed a genetic analysis in mucor circinelloides that revealed an inhibitory role for the non-canonical rdrp-depen ... | 2017 | 28339467 |
| anthropogenic impact on environmental filamentous fungi communities along the mediterranean littoral. | we hypothesised that anthropogenic influences impact the filamentous fungi community structure and that particular species or species patterns might serve as markers to characterise ecosystems. this study aimed to describe the filamentous fungi community structure in various biotopes along the mediterranean shore that were exposed to various levels of anthropogenic influence. we sampled filamentous fungi from yellow-legged gull faecal samples at five study sites along the mediterranean littoral ... | 2017 | 28276198 |
| enhanced bioremediation of lead-contaminated soil by solanum nigrum l. with mucor circinelloides. | strain selected from mine tailings in anshan for pb bioremediation was characterized at the genetic level by internal transcribed spacer (its) sequencing. results revealed that the strain belongs to mucor circinelloides. bioremediation of lead-contaminated soil was conducted using solanum nigrum l. combined with m. circinelloides. the removal efficacy was in the order microbial/phytoremediation > phytoremediation > microbial remediation > control. the bioremediation rates were 58.6, 47.2, and 40 ... | 2017 | 28251531 |
| ftir spectroscopy for evaluation and monitoring of lipid extraction efficiency for oleaginous fungi. | to assess whether fourier transform infrared (ftir) spectroscopy could be used to evaluate and monitor lipid extraction processes, the extraction methods of folch, bligh and lewis were used. biomass of the oleaginous fungi mucor circinelloides and mortierella alpina were employed as lipid-rich material for the lipid extraction. the presence of lipids was determined by recording infrared spectra of all components in the lipid extraction procedure, such as the biomass before and after extraction, ... | 2017 | 28118388 |
| rna interference in fungi: retention and loss. | rna interference (rnai) is a mechanism conserved in eukaryotes, including fungi, that represses gene expression by means of small noncoding rnas (srnas) of about 20 to 30 nucleotides. its discovery is one of the most important scientific breakthroughs of the past 20 years, and it has revolutionized our perception of the functioning of the cell. initially described and characterized in neurospora crassa, the rnai is widespread in fungi, suggesting that it plays important functions in the fungal k ... | 2016 | 28087943 |
| generation of lycopene-overproducing strains of the fungus mucor circinelloides reveals important aspects of lycopene formation and accumulation. | to generate lycopene-overproducing strains of the fungus mucor circinelloides with interest for industrial production and to gain insight into the catalytic mechanism of lycopene cyclase and regulatory process during lycopene overaccumulation. | 2017 | 27909822 |
| feeding horses with industrially manufactured pellets with fungal spores to promote nematode integrated control. | the usefulness of pellets industrially manufactured with spores of parasiticide fungi as a contribution to integrated nematode control was assessed in grazing horses throughout sixteen months. two groups of 7 pura raza galega autochthonous horses (g-t and g-p) were dewormed pour-on (1mg ivermectin/kg bw) at the beginning of the trial, and other group (g-c) remained untreated. the g-p was provided daily with commercial pellets to which was added a mixture of fungal spores during the industrial ma ... | 2016 | 27809976 |
| monotherapy or combination therapy of isavuconazole and micafungin for treating murine mucormycosis. | previously we demonstrated the benefit of isavuconazole in treating murine mucormycosis due to rhizopus. we wanted to determine the efficacy of isavuconazole in treating murine mucormycosis caused by mucor, the second most common cause of the disease. furthermore, because we previously determined that rhizopus possesses the target enzyme for echinocandins and micafungin has activity against murine mucormycosis, we compared the activity of combination therapy (isavuconazole + micafungin) with pla ... | 2017 | 27798213 |
| detection of the dimorphic phases of mucor circinelloides in blood cultures from an immunosuppressed female. | mucormycosis fungemia is rarely documented since blood cultures are nearly always negative. we describe a case of mucor circinelloides fungemia in a patient with a history of a sinus infection, sarcoidosis, and igg deficiency. the identity of the isolate was supported by its microscopic morphology and its ability to convert into yeast forms under anaerobic conditions. the early detection, initiation of liposomal amphotericin b treatment, and reversal of underlying predisposing risk factors resul ... | 2016 | 27777804 |
| antifungal activity of rhamnolipids against dimorphic fungi. | in this paper, rhamnolipids are investigated, for the first time, for their feasibility for inhibiting dimorphic fungi. rhamnolipids were found to effectively inhibit a dimorphic fungus isolated from tomato plants which was identified as mucor circinelloides according to characterizations by morphologies as well as 28s rdna sequences. rhamnolipids markedly reduced growth of this fungus in both the yeast-like form and the filamentous form. such an inhibitive effect was similarly obtained with ver ... | 2016 | 27666589 |
| fungal evolution: mucor and phycomyces see double. | newly available genome sequences of two mucoralean fungi, phycomyces blakesleeanus and mucor circinelloides, provide evidence for an ancient whole-genome duplication that contributed to the generation of expanded gene families. these fungi have robust responses to light that can be correlated with the expansion of gene networks involved in light sensing and signaling. | 2016 | 27554660 |
| selection of reference genes for quantitative real time rt-pcr during dimorphism in the zygomycete mucor circinelloides. | mucor circinelloides is a dimorphic fungal model for studying several biological processes including cell differentiation (yeast-mold transitions) as well as biodiesel and carotene production. the recent release of the first draft sequence of the m. circinelloides genome, combined with the availability of analytical methods to determine patterns of gene expression, such as quantitative reverse transcription-polymerase chain reaction (qrt-pcr), and the development of molecular genetic tools for t ... | 2015 | 25391770 |
| rhinocerebral mucor circinelloides infection in immunocompromised patient following yogurt ingestion. | the purpose of this case report is to illustrate the cause of this patient's headache and sinus pain in the setting of a unique environmental exposure: the patient ingested yogurt only days before presentation. this particular brand of yogurt caused controversy in early september 2013 when the manufacturer voluntarily recalled all flavors. the yogurt was found to be contaminated with mucor circinelloides. the recall was triggered by the fda, after receiving many complaints from consumers affecte ... | 2014 | 25252436 |
| lipase genes in mucor circinelloides: identification, sub-cellular location, phylogenetic analysis and expression profiling during growth and lipid accumulation. | lipases or triacylglycerol hydrolases are widely spread in nature and are particularly common in the microbial world. the filamentous fungus mucor circinelloides is a potential lipase producer, as it grows well in triacylglycerol-contained culture media. so far only one lipase from m. circinelloides has been characterized, while the majority of lipases remain unknown in this fungus. in the present study, 47 potential lipase genes in m. circinelloides wj11 and 30 potential lipase genes in m. circ ... | 2016 | 27535142 |
| dna barcoding in mucorales: an inventory of biodiversity. | the order mucorales comprises predominantly fast-growing saprotrophic fungi, some of which are used for the fermentation of foodstuffs but it also includes species known to cause infections in patients with severe immune or metabolic impairments. to inventory biodiversity in mucorales its barcodes of 668 strains in 203 taxa were generated covering more than two thirds of the recognised species. using the its sequences, molecular operational taxonomic units were defined by a similarity threshold ... | 2013 | 24027345 |
| vaginal colonisation by mucor circinelloides. case report with cytopathology, molecular sequencing and epidemiology. | | 2016 | 27000466 |
| genome-scale metabolic modeling of mucor circinelloides and comparative analysis with other oleaginous species. | we present a novel genome-scale metabolic model iwv1213 of mucor circinelloides, which is an oleaginous fungus for industrial applications. the model contains 1213 genes, 1413 metabolites and 1326 metabolic reactions across different compartments. we demonstrate that iwv1213 is able to accurately predict the growth rates of m. circinelloides on various nutrient sources and culture conditions using flux balance analysis and phenotypic phase plane analysis. comparative analysis of three oleaginous ... | 2016 | 26911256 |
| proteomics analysis of high lipid-producing strain mucor circinelloides wj11: an explanation for the mechanism of lipid accumulation at the proteomic level. | the oleaginous fungus, mucor circinelloides, is attracting considerable interest as it produces oil rich in γ-linolenic acid. nitrogen (n) deficiency is a common strategy to trigger the lipid accumulation in oleaginous microorganisms. although a simple pathway from n depletion in the medium to lipid accumulation has been elucidated at the enzymatic level, global changes at protein levels upon n depletion have not been investigated. in this study, we have systematically analyzed the changes at th ... | 2016 | 26867592 |
| phylogenetic analysis of fungal heterotrimeric g protein-encoding genes and their expression during dimorphism in mucor circinelloides. | in fungi, heterotrimeric g proteins are key regulators of biological processes such as mating, virulence, morphology, among others. mucor circinelloides is a model organism for many biological processes, and its genome contains the largest known repertoire of genes that encode putative heterotrimeric g protein subunits in the fungal kingdom: twelve gα (mcgpa1-12), three gβ (mcgpb1-3), and three gγ (mcgpg1-3). phylogenetic analysis of fungal gα showed that they are divided into four distinct grou ... | 2015 | 26615741 |
| rnai pathways in mucor: a tale of proteins, small rnas and functional diversity. | the existence of an rna-mediated silencing mechanism in the opportunistic fungal pathogen mucor circinelloides was first described in the early 2000. since then, mucor has reached an outstanding position within the fungal kingdom as a model system to achieve a deeper understanding of regulation of endogenous functions by the rna interference (rnai) machinery. m. circinelloides combines diverse components of its rnai machinery to carry out functions not only limited to the defense against invasiv ... | 2016 | 26593631 |
| role of malic enzyme during fatty acid synthesis in the oleaginous fungus mortierella alpina. | the generation of nadph by malic enzyme (me) was postulated to be a rate-limiting step during fatty acid synthesis in oleaginous fungi, based primarily on the results from research focusing on me in mucor circinelloides. this hypothesis is challenged by a recent study showing that leucine metabolism, rather than me, is critical for fatty acid synthesis in m. circinelloides. to clarify this, the gene encoding me isoform e from mortierella alpina was homologously expressed. me overexpression incre ... | 2014 | 24532075 |
| role of malate transporter in lipid accumulation of oleaginous fungus mucor circinelloides. | fatty acid biosynthesis in oleaginous fungi requires the supply of reducing power, nadph, and the precursor of fatty acids, acetyl-coa, which is generated in the cytosol being produced by atp: citrate lyase which requires citrate to be, transported from the mitochondrion by the citrate/malate/pyruvate transporter. this transporter, which is within the mitochondrial membrane, transports cytosolic malate into the mitochondrion in exchange for mitochondrial citrate moving into the cytosol (fig. 1). ... | 2016 | 26512004 |
| distinct rnai pathways in the regulation of physiology and development in the fungus mucor circinelloides. | the basal fungus mucor circinelloides has become, in recent years, a valuable model to study rna-mediated gene silencing or rna interference (rnai). serendipitously discovered in the late 1900s, the gene silencing in m. circinelloides is a landscape of consensus and dissents. although similar to other classical fungal models in the basic design of the essential machinery that is responsible for silencing of gene expression, the existence of small rna molecules of different sizes generated during ... | 2015 | 26410030 |
| a zebrafish larval model reveals early tissue-specific innate immune responses to mucor circinelloides. | mucormycosis is an emerging fungal infection that is clinically difficult to manage, with increasing incidence and extremely high mortality rates. individuals with diabetes, suppressed immunity or traumatic injury are at increased risk of developing disease. these individuals often present with defects in phagocytic effector cell function. research using mammalian models and phagocytic effector cell lines has attempted to decipher the importance of the innate immune system in host defence agains ... | 2015 | 26398938 |
| complete genome sequence of a high lipid-producing strain of mucor circinelloides wj11 and comparative genome analysis with a low lipid-producing strain cbs 277.49. | the genome of a high lipid-producing fungus mucor circinelloides wj11 (36% w/w lipid, cell dry weight, cdw) was sequenced and compared with that of the low lipid-producing strain, cbs 277.49 (15% w/w lipid, cdw), which had been sequenced by joint genome institute. the wj11 genome assembly size was 35.4 mb with a g+c content of 39.7%. the general features of wj11 and cbs 277.49 indicated that they have close similarity at the level of gene order and gene identity. whole genome alignments with mau ... | 2015 | 26352831 |
| screening of phosphorus-accumulating fungi and their potential for phosphorus removal from waste streams. | while bacteria have been primarily studied for phosphorus (p) removal in wastewater treatment, fungi and their ability to accumulate intracellular polyphosphate are less investigated. p-accumulating fungal strains were screened from soybean plants and surrounding soil by flask cultivation with potato dextrose broth and kh2po4 in this study. mucor circinelloides was selected for its high efficiency in p removal efficiency and high cellular p content. neisser staining and growth-curve analysis con ... | 2015 | 26280802 |
| comparison of biochemical activities between high and low lipid-producing strains of mucor circinelloides: an explanation for the high oleaginicity of strain wj11. | the oleaginous fungus, mucor circinelloides, is one of few fungi that produce high amounts of γ-linolenic acid (gla); however, it usually only produces <25% lipid. nevertheless, a new strain (wj11) isolated in this laboratory can produce lipid up to 36% (w/w) cell dry weight (cdw). we have investigated the potential mechanism of high lipid accumulation in m. circinelloides wj11 by comparative biochemical analysis with a low lipid-producing strain, m. circinelloides cbs 277.49, which accumulates ... | 2015 | 26046932 |
| [isolation of filamentous fungi capable of enhancing sludge dewaterability and study of mechanisms responsible for the sludge dewaterability enhancement]. | to study the influence of filamentous fungi on the sludge dewaterability is very significant for the development of biological treatment methods for enhancing sludge dewaterability. in this study, filamentous fungi capable of enhancing sludge dewaterability were isolated from sewage sludge and the related mechanisms responsible for the sludge dewaterability enhancement were investigated. a filamentous fungus mucor circinelloides zg-3 was successfully isolated from sludge, and sludge dewaterabili ... | 2015 | 26031090 |
| calcineurin orchestrates dimorphic transitions, antifungal drug responses and host-pathogen interactions of the pathogenic mucoralean fungus mucor circinelloides. | calcineurin plays essential roles in virulence and growth of pathogenic fungi and is a target of the natural products fk506 and cyclosporine a. in the pathogenic mucoralean fungus mucor circinelloides, calcineurin mutation or inhibition confers a yeast-locked phenotype indicating that calcineurin governs the dimorphic transition. genetic analysis in this study reveals that two calcineurin a catalytic subunits (out of three) are functionally diverged. homology modeling illustrates modes of resist ... | 2015 | 26010100 |
| identification of fungal ene-reductase activity by means of a functional screening. | bioeconomy stresses the need of green processes promoting the development of new methods for biocatalyzed alkene reductions. a functional screening of 28 fungi belonging to ascomycota, basidiomycota, and zygomycota isolated from different habitats was performed to analyze their capability to reduce c=c double bonds towards three substrates (cyclohexenone, α-methylnitrostyrene, and α-methylcinnamaldehyde) with different electron-withdrawing groups, i.e., ketone, nitro, and aldehyde, respectively. ... | 2015 | 25986545 |
| the rnai machinery controls distinct responses to environmental signals in the basal fungus mucor circinelloides. | rna interference (rnai) is a conserved mechanism of genome defence that can also have a role in the regulation of endogenous functions through endogenous small rnas (esrnas). in fungi, knowledge of the functions regulated by esrnas has been hampered by lack of clear phenotypes in most mutants affected in the rnai machinery. mutants of mucor circinelloides affected in rnai genes show defects in physiological and developmental processes, thus making mucor an outstanding fungal model for studying e ... | 2015 | 25880254 |
| rapid screening for human-pathogenic mucorales using rolling circle amplification. | mucormycosis has emerged as a relatively common severe mycosis in patients with haematological and allogeneic stem cell transplantation. source of transmission is from unidentified sources in the environment. early diagnosis of infection and its source of contamination are paramount for rapid and appropriate therapy. in this study, rolling circle amplification (rca) is introduced as a sensitive, specific and reproducible isothermal dna amplification technique for rapid molecular identification o ... | 2014 | 25187214 |
| zygomycota associated with traditional meju, a fermented soybean starting material for soy sauce and soybean paste. | various zygomycota species were detected during a study of the mycobiota of meju, a brick of dried fermented soybeans, used in korean cuisine. two hundred and sixty-eight strains were isolated from 98 finished meju products collected in various regions of korea from 2009 to 2011, and 96 strains were isolated from in-process meju on various farms from 2010 to 2011. the isolated zygomycota were identified using phenotypic characteristics combined with dna sequences of the internal transcribed spac ... | 2012 | 22752901 |
| biosynthesis, characterization and enzymatic transesterification of single cell oil of mucor circinelloides--a sustainable pathway for biofuel production. | the filamentous fungus mucor circinelloides urm 4182 was tested to determine its ability to produce single-cell oil suitable for obtaining biodiesel. cell growth and lipid accumulation were investigated in a medium containing glucose as the main carbon source. a microwave-assisted ethanol extraction technique (microwave power ⩽200 w, 50-60 °c) was established and applied to lipid extraction from the fungal hyphae to obtain high lipid concentration (44%wt) of the dry biomass, which was considerab ... | 2015 | 25625466 |
| overexpression of malic enzyme (me) of mucor circinelloides improved lipid accumulation in engineered rhodotorula glutinis. | the oleaginous yeast rhodotorula glutinis has been known to be a potential feedstock for lipid production. in the present study, we investigated the enhancement of expression of malic enzyme (me; nadp(+) dependent; ec 1.1.1.40) from mucor circinelloides as a strategy to improve lipid content inside the yeast cells. the 26s rdna and 5.8s rdna gene fragments isolated from rhodotorula glutinis were used for homologous integration of me gene into r. glutinis chromosome under the control of the const ... | 2013 | 23179623 |
| a non-canonical rna silencing pathway promotes mrna degradation in basal fungi. | the increasing knowledge on the functional relevance of endogenous small rnas (esrnas) as riboregulators has stimulated the identification and characterization of these molecules in numerous eukaryotes. in the basal fungus mucor circinelloides, an emerging opportunistic human pathogen, esrnas that regulate the expression of many protein coding genes have been described. these esrnas share common machinery for their biogenesis consisting of an rnase iii endonuclease dicer, a single argonaute prot ... | 2015 | 25875805 |
| when you can't see the wood for the trees. mucor circinelloides: a rare case of primary cutaneous zygomycosis. | a patient with refractory diffuse lymphoma treated for pulmonary invasive aspergillosis developed a concomitant primary cutaneous mucormycosis. the mucormycete was identified by sequencing as mucor circinelloides. this case confirms the importance of a rapid pathogen diagnosis in immunocompromised patients and the usefulness of molecular methods for identification of rare fungal species. | 2015 | 25840851 |
| comparative studies of oleaginous fungal strains (mucor circinelloides and trichoderma reesei) for effective wastewater treatment and bio-oil production. | biological wastewater treatment typically requires the use of bacteria for degradation of carbonaceous and nitrogenous compounds present in wastewater. the high lipid containing biomass can be used to extract oil and the contents can be termed as bio-oil (or biodiesel or myco-diesel after transesterification). the separate experiments were conducted on actual wastewater samples with 5% v/v inoculum of mucor circinelloides mtcc1297 and trichoderma reesei ncim992 strains. the observed reductions i ... | 2014 | 25530884 |
| expression of xanthophyllomyces dendrorhous cytochrome-p450 hydroxylase and reductase in mucor circinelloides. | carotenoids are natural pigments that act as powerful antioxidants and have various beneficial effects on human and animal health. mucor circinelloides (mucoromycotina) is a carotenoid producing zygomycetes fungus, which accumulates β-carotene as the main carotenoid but also able to produce the hydroxylated derivatives of β-carotene (i.e. zeaxanthin and β-cryptoxanthin) in low amount. these xanthophylls, together with the ketolated derivatives of β-carotene (such as canthaxanthin, echinenone and ... | 2015 | 25504221 |
| identification of a β-glucosidase from the mucor circinelloides genome by peptide pattern recognition. | mucor circinelloides produces plant cell wall degrading enzymes that allow it to grow on complex polysaccharides. although the genome of m. circinelloides has been sequenced, only few plant cell wall degrading enzymes are annotated in this species. we applied peptide pattern recognition, which is a non-alignment based method for sequence analysis to map conserved sequences in glycoside hydrolase families. the conserved sequences were used to identify similar genes in the m. circinelloides genome ... | 2014 | 25442948 |
| effects of 20 standard amino acids on the growth, total fatty acids production, and γ-linolenic acid yield in mucor circinelloides. | twenty standard amino acids were examined as single nitrogen source on the growth, total fatty acids production, and yield of γ-linolenic acid (gla) in mucor circinelloides. of the amino acids, tyrosine gave the highest biomass and lipid accumulation and thus resulted in a high gla yield with respective values of 17.8 g/l, 23 % (w/w, dry cell weight, dcw), and 0.81 g/l, which were 36, 25, and 72 % higher than when the fungus was grown with ammonium tartrate. to find out the potential mechanism u ... | 2014 | 25117649 |
| lipid accumulation by pelletized culture of mucor circinelloides on corn stover hydrolysate. | microbial oil accumulated by fungal cells is a potential feedstock for biodiesel production, and lignocellulosic materials can serve as the carbon source to support the fungal growth. the dilute acid pretreatment of corn stover can effectively break down its lignin structure, and this process generates a hydrolysate containing mostly xylose at very dilute concentration and numerous by-products that may significantly inhibit the cell growth. this study utilized corn stover hydrolysate as the cult ... | 2014 | 25080382 |
| expansion of signal transduction pathways in fungi by extensive genome duplication. | plants and fungi use light and other signals to regulate development, growth, and metabolism. the fruiting bodies of the fungus phycomyces blakesleeanus are single cells that react to environmental cues, including light, but the mechanisms are largely unknown [1]. the related fungus mucor circinelloides is an opportunistic human pathogen that changes its mode of growth upon receipt of signals from the environment to facilitate pathogenesis [2]. understanding how these organisms respond to enviro ... | 2016 | 27238284 |
| detection of 3-nitropropionic acid and cytotoxicity inmucor circinelloides. | mucorales are regarded as the aetiological agents of mucormycosis. their capabilities to produce mycotoxins are not profoundly investigated, in contrast to those of the fungi from the generapenicillium, aspergillus, orfusarium. the aim of this study was to isolate and identify fungi of the order mucorales and investigate mycotoxins production. twelve samples of visibly moulded grass silage and eight samples of damaged whole crop maize silage were analysed. malt extract agar plates were used for ... | 2008 | 23604749 |
| the selectivity of some fungal lipases. | selectivity is one of the most important lipase properties which depends on a wide range of factors. in order to choose the right enzyme for a special purpose, it is necessary to check its selectivity. fatty acid selectivity of lipases determined for natural substrales was different from that determined for p-nitrophenyl esters and those determined for each substrate. enantoiselectivity of lipase from mucor circinelloides (mcl) determined for 2 was over 100 (e > > 100). in this case, inversion o ... | 2003 | 24757816 |
| characterization and control of mucor circinelloides spoilage in yogurt. | consumer confidence in the food industry is severely affected by large-scale spoilage incidents. however, relatively little research exists on spoilage potential of members of the fungal subphylum mucormycotina (e.g. mucor), which includes dimorphic spoilage organisms that can switch between a yeast-like and hyphal phase depending on environmental conditions. the presence of mucor circinelloides in yogurt may not cause spoilage, but growth and subsequent changes in quality (e.g. container bloati ... | 2016 | 27085035 |
| analysis of a food-borne fungal pathogen outbreak: virulence and genome of a mucor circinelloides isolate from yogurt. | food-borne pathogens are ongoing problems, and new pathogens are emerging. the impact of fungi, however, is largely underestimated. recently, commercial yogurts contaminated with mucor circinelloides were sold, and >200 consumers became ill with nausea, vomiting, and diarrhea. mucoralean fungi cause the fatal fungal infection mucormycosis, whose incidence has been continuously increasing. in this study, we isolated an m. circinelloides strain from a yogurt container, and multilocus sequence typi ... | 2014 | 25006230 |
| mucor circinelloides whole-cells as a biocatalyst for the production of ethyl esters based on babassu oil. | the intracellular lipase production by mucor circinelloides urm 4182 was investigated through a step-by-step strategy to attain immobilized whole-cells with high lipase activity. physicochemical parameters, such as carbon and nitrogen sources, inoculum size and aeration, were studied to determine the optimum conditions for both lipase production and immobilization in polyurethane support. olive oil and soybean peptone were found to be the best carbon and nitrogen sources, respectively, to enhanc ... | 2014 | 24958521 |
| genome sequence and transcriptome analyses of the thermophilic zygomycete fungus rhizomucor miehei. | the zygomycete fungi like rhizomucor miehei have been extensively exploited for the production of various enzymes. as a thermophilic fungus, r. miehei is capable of growing at temperatures that approach the upper limits for all eukaryotes. to date, over hundreds of fungal genomes are publicly available. however, zygomycetes have been rarely investigated both genetically and genomically. | 2014 | 24746234 |
| statistical analysis and modeling of pelletized cultivation of mucor circinelloides for microbial lipid accumulation. | microbial oil accumulation via oleaginous fungi has some potential benefits because filamentous fungi can form pellets during cell growth and these pellets are easier to harvest from the culture broth than individual cells. this research studied the effect of various culture conditions on the pelletized cell growth of mucor circinelloides and its lipid accumulation. the results showed that cell pelletization was positively correlated to biomass accumulation; however, pellet size was negatively c ... | 2014 | 24549800 |
| purification and characteristics of an inducible by polycyclic aromatic hydrocarbons nadp(+)-dependent naphthalenediol dehydrogenase (ndd) in mucor circinelloides yr-1. | we detected nadp(+)-dependent dihydrodiol dehydrogenase (dd) activity in a cell-free extract from mucor circinelloides yr-1, after high-speed centrifugation. we analyzed the enzymatic activity in the cytosolic fraction by zymograms, as described previously, and eight different dd activity bands were revealed. five constitutive dd activities (dd1-5) were present when glucose was used as carbon source and three inducible activities (ndd, pdd1 and pdd2) when aromatic hydrocarbon compounds were used ... | 2014 | 24530565 |
| repertoire of malic enzymes in yeast and fungi: insight into their evolutionary functional and structural significance. | malic enzyme (me) is one of the important enzymes for furnishing the cofactor nad(p)h for the biosynthesis of fatty acids and sterols. due to the existence of multiple me isoforms in a range of oleaginous microbes, a molecular basis for the evolutionary relationships amongst the enzymes in oleaginous fungi was investigated using sequence analysis and structural modelling. evolutionary distance and structural characteristics were used to discriminate the mes of yeasts and fungi into several group ... | 2013 | 24065718 |
| two new β-glucosidases from ethanol-fermenting fungus mucor circinelloides nbrc 4572: enzyme purification, functional characterization, and molecular cloning of the gene. | two β-glucosidases (bgls 1 and 2) were purified to homogeneity from the extracellular enzyme preparations of the ethanol-fermenting mucor circinelloides nbrc 4572 statically grown on rice straw. bgls 1 and 2 are monomeric glycoproteins whose apparent molecular masses (ms) are around 78 kda, which decreased by approximately 10 kda upon enzymatic deglycosylation. both bgls showed similar enzyme characteristics in optimal temperature and ph, stability, and inhibitors. they were active against a wid ... | 2013 | 24061417 |
| calcineurin plays key roles in the dimorphic transition and virulence of the human pathogenic zygomycete mucor circinelloides. | many pathogenic fungi are dimorphic and switch between yeast and filamentous states. this switch alters host-microbe interactions and is critical for pathogenicity. however, in zygomycetes, whether dimorphism contributes to virulence is a central unanswered question. the pathogenic zygomycete mucor circinelloides exhibits hyphal growth in aerobic conditions but switches to multi-budded yeast growth under anaerobic/high co₂ conditions. we found that in the presence of the calcineurin inhibitor fk ... | 2013 | 24039585 |
| genomic, proteomic, and biochemical analyses of oleaginous mucor circinelloides: evaluating its capability in utilizing cellulolytic substrates for lipid production. | lipid production by oleaginous microorganisms is a promising route to produce raw material for the production of biodiesel. however, most of these organisms must be grown on sugars and agro-industrial wastes because they cannot directly utilize lignocellulosic substrates. we report the first comprehensive investigation of mucor circinelloides, one of a few oleaginous fungi for which genome sequences are available, for its potential to assimilate cellulose and produce lipids. our genomic analysis ... | 2013 | 24023719 |
| a single argonaute gene participates in exogenous and endogenous rnai and controls cellular functions in the basal fungus mucor circinelloides. | the mechanism of rnai is well described in metazoans where it plays a role in diverse cellular functions. however, although different classes of endogenous small rnas (esrnas) have been identified in fungi, their biological roles are poorly described due, in part, to the lack of phenotype of mutants affected in the biogenesis of these esrnas. argonaute proteins are one of the key components of the rnai pathways, in which different members of this protein family participate in the biogenesis of a ... | 2013 | 23935973 |
| effect of immobilized polygalacturonase from mucor circinelloides itcc-6025 on wine fermentation. | pectinases are among the most widely distributed enzymes in bacteria, fungi, and plants. almost all the commercial preparations of pectinases are produced from fungal sources. mucor circinelloides itcc-6025 produced polygalacturonase when grown in riviere's medium containing pectin (methyl ester) as the sole source of carbon. immobilization of purified polygalacturonase was done on silica gel with 86% efficiency. the enzyme took 60 min to bind maximally on the support. the immobilized enzyme sho ... | 2013 | 23600573 |
| antimicrobial activity of water and acetone extracts of some eucomis taxa. | to evaluate the antibacterial and antifungal properties of acetone and water extracts of selected eucomis taxa. | 2017 | 29080618 |
| entomophthoromycosis and mucormycosis as causes of pneumonia in vietnamese potbellied pigs. | two vietnamese potbellied pigs ( sus scrofa) had respiratory disease and, on autopsy, both pigs had large masses in the lungs and thoracic cavity. microscopically, pulmonary and pleural masses contained large areas with hyphae surrounded by hypereosinophilic cellular debris rimmed by abundant eosinophils, lymphocytes, plasma cells, and histiocytes with occasional multinucleate giant cells. the hypereosinophilic debris usually formed tight cuffs, or "sleeves" around the hyphae, compatible with sp ... | 2018 | 29034814 |
| occurrence and species distribution of pathogenic mucorales in unselected soil samples from france. | mucormycosis is a life-threatening invasive fungal disease that affects a variety of patient groups. although mucorales are mostly opportunistic pathogens originating from soil or decaying vegetation, there are currently few data on prevalence of this group of fungi in the environment. the aim of the present study was to assess the prevalence and diversity of species of mucorales from soil samples collected in france. two grams of soil were homogenized in sterile saline and plated on sabouraud d ... | 2017 | 28992337 |
| genome-scale analysis of the metabolic networks of oleaginous zygomycete fungi. | microbial lipids are becoming an attractive option for the industrial production of foods and oleochemicals. to investigate the lipid physiology of the oleaginous microorganisms, at the system level, genome-scale metabolic networks of mortierella alpina and mucor circinelloides were constructed using bioinformatics and systems biology. as scaffolds for integrated data analysis focusing on lipid production, consensus metabolic routes governing fatty acid synthesis, and lipid storage and mobilisat ... | 2013 | 23541380 |
| effect of potassium ions on protoplast generation during yeast induction from mucor circinelloides tieghem. | mucor circinelloides aerobically exhibits coenocytic thallic growth habit with straight and circinate sporangiophores which culminate in globose or pyriform columellae enclosed within sporangial walls. it undergoes dimorphic switch with its conversion to multipolar budding yeast-like cells or thallic conidia. this paper confirms the induction of plurality of reproductive structures of the pleomorphic microorganism in minimal medium. furthermore, construction of ph differentials at inflection poi ... | 2013 | 25969779 |
| isolation and identification of fungi from a meju contaminated with aflatoxins. | a home-made meju sample contaminated naturally with aflatoxins was used for isolation of fungal strains. overall, 230 fungal isolates were obtained on dichloran rosebengal chloramphenicol (drbc) and dichloran 18% glycerol (dg18) agar plates. morphological characteristics and molecular analysis of a partial beta-tubulin gene and the internal transcribed spacer (its) of rdna were used for the identification of the isolates. the fungal isolates were divided into 7 genera: aspergillus, eurotium, pen ... | 2012 | 23221538 |
| malic enzyme activity is not the only bottleneck for lipid accumulation in the oleaginous fungus mucor circinelloides. | commercial interest in microbial lipids is increasing due to their potential use as feedstock for biodiesel production. the supply of nadph generated by malic enzyme (me; nadp+-dependent; ec 1.1.1.40) has been postulated as being the rate-limiting step for fatty acid biosynthesis in oleaginous fungi, based mainly on data from the zygomycete mucor circinelloides studies. this fungus contains five genes that code for six different me isoforms. one of these genes, mala, codes for the isoforms iii a ... | 2013 | 23053085 |
| metabolic engineering of mucor circinelloides for zeaxanthin production. | mucor circinelloides is a β-carotene producing zygomycete amenable to metabolic engineering using molecular tools. the crts gene of the heterobasidiomycetous yeast xanthophyllomyces dendrorhous encodes the enzymatic activities β-carotene hydroxylase and ketolase, allowing this yeast to produce the xanthophyll called astaxanthin. here we describe the fermentation of x. dendrorhous in astaxanthin producing conditions to purify mrna for the cloning of the cdna from the crts gene by rt-pcr. further ... | 2012 | 22711122 |
| gene fusions for the directed modification of the carotenoid biosynthesis pathway in mucor circinelloides. | several fungal species, particularly some included in the mucorales, have been used to develop fermentation processes for the production of β-carotene. oxygenated derivatives of β-carotene are more valuable products, and the preference by the market of carotenoids from biological sources has increased the research in different carotenoid-producing organisms. we currently use mucor circinelloides as a model organism to develop strains able to produce new, more valuable, and with an increased cont ... | 2012 | 22711120 |
| molecular tools for carotenogenesis analysis in the zygomycete mucor circinelloides. | the carotene producer fungus mucor circinelloides is the zygomycete more amenable to genetic manipulations by using molecular tools. since the initial development of an effective procedure of genetic transformation, more than two decades ago, the availability of new molecular approaches such as gene replacement techniques and gene expression inactivation by rna silencing, in addition to the sequencing of its genome, has made mucor a valuable organism for the study of a number of processes. here ... | 2012 | 22711119 |
| protein kinase a regulatory subunit isoforms regulate growth and differentiation in mucor circinelloides: essential role of pkar4. | the protein kinase a (pka) signaling pathway plays a role in regulating growth and differentiation in the dimorphic fungus mucor circinelloides. pka holoenzyme is comprised of two catalytic (c) and two regulatory (r) subunits. in m. circinelloides, four genes encode the pkar1, pkar2, pkar3, and pkar4 isoforms of r subunits. we have constructed null mutants and demonstrate that each isoform has a different role in growth and differentiation. the most striking finding is that pkar4 is an essential ... | 2012 | 22635921 |
| annotation and analysis of malic enzyme genes encoding for multiple isoforms in the fungus mucor circinelloides cbs 277.49. | based on the newly-released genomic data of mucor circinelloides cbs 277.49, we have annotated five genes encoding for malic enzyme: all code for proteins that contain conserved domains/motifs for malic acid binding, nad(+) binding and nad(p)(+) binding. phylogenetic analysis for malic enzyme genes showed that genes id 78524 and 11639 share ~80% amino acid identity and are grouped in cluster 1; genes id 182779, 186772 and 116127 share ~66% amino acid identity are grouped in cluster 2. genes id 7 ... | 2012 | 22367279 |
| in vitro and in vivo activities of posaconazole and amphotericin b in a murine invasive infection by mucor circinelloides: poor efficacy of posaconazole. | the in vitro susceptibility of 17 strains of mucor circinelloides to amphotericin b and posaconazole was ascertained by using broth microdilution and disk diffusion methods and by determining the minimal fungicidal concentration (mfc). we evaluated the efficacy of posaconazole at 40 mg/kg of body weight/day and amphotericin b at 0.8 mg/kg/day in a neutropenic murine model of disseminated infection by m. circinelloides by using 6 different strains tested previously in vitro. in general, most of t ... | 2012 | 22290952 |
| lipid formation and γ-linolenic acid production by mucor circinelloides and rhizopus sp., grown on vegetable oil. | the fungi strains were tested in bioscreen automated system to select the best nutritional source. following, shaking submserse cultures were studied in media containing sole carbon or nitrogen source. the growth of these strains improved in media containing vegetable oil, with high concentration of lipids. the high concentration of γ-linolenic acid was obtained with m. circinelloides in culture containing sesame oil. | 2009 | 24031370 |
| antifungal drug resistance evoked via rnai-dependent epimutations. | microorganisms evolve via a range of mechanisms that may include or involve sexual/parasexual reproduction, mutators, aneuploidy, hsp90 and even prions. mechanisms that may seem detrimental can be repurposed to generate diversity. here we show that the human fungal pathogen mucor circinelloides develops spontaneous resistance to the antifungal drug fk506 (tacrolimus) via two distinct mechanisms. one involves mendelian mutations that confer stable drug resistance; the other occurs via an epigenet ... | 2014 | 25079329 |
| construction of a recyclable genetic marker and serial gene deletions in the human pathogenic mucorales mucor circinelloides. | mucor circinelloides is a human pathogen, biofuel producer, and model system that belongs to a basal fungal lineage; however, the genetics of this fungus are limited. in contrast to ascomycetes and basidiomycetes, basal fungal lineages have been understudied. this may be caused by a lack of attention given to these fungi, as well as limited tools for genetic analysis. nonetheless, the importance of these fungi as pathogens and model systems has increased. m. circinelloides is one of a few geneti ... | 2017 | 28476909 |
| mucor circinelloides induces platelet aggregation through integrin αiibβ3 and fcγriia. | thrombosis is a hallmark of the fatal fungal infection mucormycosis. yet, the platelet activation pathway in response to mucormycetes is unknown. in this study we determined the platelet aggregation potential of mucor circinelloides (m. circinelloides) nrrl3631, characterized the signaling pathway facilitating aggregation in response to fungal spores, and identified the influence of the spore developmental stage upon platelet aggregation potential. using impedance and light-transmission aggregom ... | 2018 | 29297721 |
| control of morphology and virulence by adp-ribosylation factors (arf) in mucor circinelloides. | mucor circinelloides is a dimorphic fungus used to study cell differentiation that has emerged as a model to characterize mucormycosis. in this work, we identified four adp-ribosylation factor (arf)-encoding genes (arf1-arf4) and study their role in the morphogenesis and virulence. arfs are key regulators of the vesicular trafficking process and are associated with both growth and virulence in fungi. arf1 and arf2 share 96% identity and arf3 and arf4 share 89% identity, which suggests that the g ... | 2017 | 29264641 |
| identification and characterization of diacylglycerol acyltransferase from oleaginous fungus mucor circinelloides. | acyl-coa:diacylglycerol acyltransferase (dgat) is a pivotal regulator of triacylglycerol (tag) synthesis. the oleaginous fungus mucor circinelloides has four putative dgats: mcdgat1a, mcdgat1b, mcdgat2a, and mcdgat2b, classified into the dgat1 and dgat2 subfamilies, respectively. to identify and characterize dgats in m. circinelloides, these four genes were expressed in saccharomyces cerevisiae h1246 (tag-deficient quadruple mutant), individually. tag biosynthesis was restored only by the expres ... | 2018 | 29260551 |
| development of a plasmid free crispr-cas9 system for the genetic modification of mucor circinelloides. | mucor circinelloides and other members of mucorales are filamentous fungi, widely used as model organisms in basic and applied studies. although genetic manipulation methods have been described for some mucoral fungi, construction of stable integrative transformants by homologous recombination has remained a great challenge in these organisms. in the present study, a plasmid free crispr-cas9 system was firstly developed for the genetic modification of a mucoral fungus. the described method offer ... | 2017 | 29196656 |
| intrinsic short-tailed azole resistance in mucormycetes is due to an evolutionary conserved aminoacid substitution of the lanosterol 14α-demethylase. | mucormycoses are emerging and potentially lethal infections. an increase of breakthrough infections has been found in cohorts receiving short-tailed azoles prophylaxis (e.g. voriconazole (vcz)). although vcz is ineffective in vitro and in vivo, long-tailed triazoles such as posaconazole remain active against mucormycetes. our goal was to validate the molecular mechanism of resistance to short-tailed triazoles in mucorales. the paralogous cytochrome p450 genes (cyp51 f1 and cyp51 f5) of rhizopus ... | 2017 | 29162893 |
| biosurfactant production by mucor circinelloides on waste frying oil and possible uses in crude oil remediation. | biosurfactants are biocompatible surface active agents which many microorganisms produce. this study investigated the production of biosurfactants by mucor circinelloides. the effects of different factors on biosurfactant production, including carbon sources and concentrations, nitrogen sources, and iron (ii) concentration, were studied and the optimum condition determined. finally, the strain's ability to remove the crude oil and its relationship with biosurfactant production was evaluated. the ... | 2017 | 28991787 |
| antioxidants potential of the filamentous fungi (mucor circinelloides). | three important strains of mucorcircinelloides grown in complete and minimal media for specified period (72 h, 120 h and 168 h) under submerged fermentation conditions were investigated for their potential antioxidants/secondary metabolite production. all mycelial extracts demonstrated effective antioxidant activities in terms of β-carotene/linoleic acid bleaching, radical scavenging, reduction of metal ions and chelating abilities against ferrous ions. different extraction methods and solvent s ... | 2017 | 28991177 |
| a protein complex bearing an oxidase with napthalene dihydrodiol dehydrogenase activity is induced in mucor circinelloides strain yr-1 during growth on polycyclic aromatic compounds. | fungi are organisms capable of growing in a myriad of conditions and respond to counteract environmental cues. several locations in the world are polluted with oil and its derivatives, and some microorganisms tolerant to these compounds have been isolated. some fungi can grow in the presence of molecules such as polycyclic aromatic hydrocarbons as sole carbon sources. in this report, we further characterized the induced enzymes with phenanthrene from mucor circinelloides yr-1 strain, isolated fr ... | 2018 | 28975438 |
| old yellow enzyme homologues in mucor circinelloides: expression profile and biotransformation. | the reduction of c=c double bond, a key reaction in organic synthesis, is mostly achieved by traditional chemical methods. therefore, the search for enzymes capable of performing this reaction is rapidly increasing. old yellow enzymes (oyes) are flavin-dependent oxidoreductases, initially isolated from saccharomyces pastorianus. in this study, the presence and activation of putative oye enzymes was investigated in the filamentous fungus mucor circinelloides, which was previously found to mediate ... | 2017 | 28935878 |