| control of charcoal rot fungus macrophomina phaseolina by extracts of datura metel. | methanolic leaf and fruit extracts of datura metel were found highly effective in suppressing against macrophomina phaseolina, the cause of charcoal rot disease. these extracts were further subjected to successive fractionation with n-hexane, chloroform, ethyl acetate and n-butanol. all the concentrations (3.125-200 mg ml(-1)) of chloroform, ethyl acetate and n-butanol fractions of leaf extract, and n-hexane fraction of fruit extract completely inhibited the target fungal growth. two compounds a ... | 2011 | 22004473 |
| High throughput screening of fungal endoglucanase activity in Escherichia coli. | Cellulase enzymes (endoglucanases, cellobiohydrolases, and ß-glucosidases) hydrolyze cellulose into component sugars, which in turn can be converted into fuel alcohols. The potential for enzymatic hydrolysis of cellulosic biomass to provide renewable energy has intensified efforts to engineer cellulases for economical fuel production. Of particular interest are fungal cellulases, which are already being used industrially for foods and textiles processing. Identifying active variants among a libr ... | 2011 | 21860379 |
| emerging phytopathogen macrophomina phaseolina: biology, economic importance and current diagnostic trends. | macrophomina phaseolina (tassi) goid. is an important phytopathogenic fungus, infecting a large number of plant species and surviving for up to 15 years in the soil as a saprophyte. although considerable research related to the biology and ecology of macrophomina has been conducted, it continues to cause huge economic losses in many crops. research is needed to improve the identification and characterization of genetic variability within their epidemiological and pathological niches. better unde ... | 2012 | 22257260 |
| transformation of pwwo in rhizobium leguminosarum dpt to engineer toluene degrading ability for rhizoremediation. | rhizoremediation of organic xenobiotics is based on interactions between plants and their associated micro-organisms. the present work was designed to engineer a bacterial system having toluene degradation ability along with plant growth promoting characteristics for effective rhizoremediation. pwwo harboring the genes responsible for toluene breakdown was isolated from pseudomonas putida mtcc 979 and successfully transformed in rhizobium dpt. this resulted in a bacterial strain (dpt(t)) which h ... | 2011 | 23729882 |
| isolation and partial characterization of antibacterial lipopeptide produced by paenibacillus polymyxa hka-15 against phytopathogen xanthomonas campestris pv. phaseoli m-5. | an antibacterial metabolite was isolated from paenibacillus polymyxa hka-15, a soybean bacterial endophyte. the purification of the crude metabolite from paenibacillus polymyxa hka-15 was done by column chromatography. in tlc, a spot with an r ( f ) value of 0.86 (±0.02) from the purified fraction showed bioactivity against xanthomonas campestris pv. phaseoli m-5. in sds-page, the purified antibiotic was separated in the molecular weight range of 3.5 kda. the exact molecular weight of the active ... | 2011 | 22805811 |
| screening of rhizobacteria for their plant growth promotion ability and antagonism against damping off and root rot diseases of broad bean (vicia faba l.). | development of microbial inoculants from rhizobacterial isolates with potential for plant growth promotion and root disease suppression require rigorous screening. fifty-four (54) fluorescent pseudomonads, out of a large collection of rhizobacteria from broad bean fields of 20 different locations within imphal valley of manipur, were initially screened for antifungal activity against macrophomina phaseolina and rhizoctonia solani, of diseased roots of broad bean and also three other reference fu ... | 2011 | 22282623 |
| synthesis, antifungal activity and structure-activity relationships of vanillin oxime-n-o-alkanoates. | vanillin oxime-n-o-alkanoates were synthesized following reaction of vanillin with hydroxylamine hydrochloride, followed by reaction of the resultant oxime with acyl chlorides. the structures of the compounds were confirmed by ir, 1h, 13c nmr and mass spectral data. the test compounds were evaluated for their in vitro antifungal activity against three phytopathogenic fungi macrophomina phaseolina, rhizoctonia solani and sclerotium rolfsii by the poisoned food technique. the moderate antifungal a ... | 2012 | 23413571 |
| xyloglucan endotransglycosylase/hydrolase genes from a susceptible and resistant jute species show opposite expression pattern following macrophomina phaseolina infection. | two of the most widely and intensively cultivated jute species, corchorus capsularis and corchorus olitorius, suffer severely from a stem rot disease caused by the fungus macrophomina phaseolina. wild jute species, c. trilocularis, shows resistance to this pathogenic fungus. in this study, the technique of differential display was applied to identify genes which are differentially expressed, under both infected and un-infected conditions, between c. trilocularis and c. olitorius var o-72. two xy ... | 2012 | 23336031 |
| in vitro and in vivo biocontrol of soil-borne phytopathogenic fungi by certain bioagents and their possible mode of action. | the study aimed to investigate new wide-spectrum biological control agents against soil-borne fungal plant pathogens. screening of 336 fungal strains and 256 bacterial strains was carried out in vitro to select the most promising isolates for controlling destructive pathogens of greenhouse-vegetables. in dual cultures, bacillus subtilis jf419701 and trichoderma harzianum jf419706 (teleomorph: hypocrea lixii) inhibited the growth of alternaria alternata, fusarium oxysporum, exserohilum rostratum, ... | 2012 | 23269217 |