Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| latency of bovine herpesvirus type 5 (bohv-5) in tonsils and peripheral blood leukocytes. | bovine herpesvirus types 1 (bohv-1) and 5 (bohv-5) can both establish latency in the trigeminal ganglion. non-neural sites of latency have been described for bohv-1 but not for bohv-5. the aim of this study was to determine whether peripheral blood leukocytes and tonsils are targets for bohv-5 infection and to establish whether all stages of that virus's infectious cycle can occur in those cell types. comparisons with bohv-1 infection of these tissues were also made in order to better understand ... | 2014 | 25155304 |
| toll-like receptor expression in the nervous system of bovine alpha-herpesvirus-infected calves. | in this study, the expression levels of viral toll-like receptors (tlrs) in the nervous system of bovine herpesvirus type 5 (bohv-5)-infected calves were investigated. a significant increase in the expression of tlrs 3 and 7-9 was found in the anterior cerebral cortex during acute infection and viral reactivation. in the trigeminal ganglia, only tlr9 expression was significantly affected. the magnitude of the increase was lower in bohv-1-infected calves, suggesting that a restricted immune respo ... | 2014 | 25172667 |
| bovine herpesvirus glycoprotein d: a review of its structural characteristics and applications in vaccinology. | the viral envelope glycoprotein d from bovine herpesviruses 1 and 5 (bohv-1 and -5), two important pathogens of cattle, is a major component of the virion and plays a critical role in the pathogenesis of herpesviruses. glycoprotein d is essential for virus penetration into permissive cells and thus is a major target for virus neutralizing antibodies during infection. in view of its role in the induction of protective immunity, gd has been tested in new vaccine development strategies against both ... | 2014 | 25359626 |
| bovine herpesvirus type 5 in semen samples from bulls in iran. | bovine herpesvirus 5 (bohv-5) is an important pathogen of the central nervous system and has already been described in the genital tract of cattle and in semen. this virus is responsible for sporadic epizootics of fatal meningoencephalitis of calves. the objective of the present study was the identification and characterization of bohv-5 in semen samples from bulls for the first time in iran. dna was extracted from bull semen samples, and the glycoprotein d (gd) gene of bohv-5 and also the thymi ... | 2015 | 25362547 |
| identification of a novel herpesvirus associated with a penile proliferative lesion in a beluga (delphinapterus leucas). | the carcass of an adult male beluga (delphinapterus leucas) was found beach cast in 2008 on the shore of the st. lawrence estuary at rivière-ouelle, quebec, canada. the carcass was transported to the faculté de médecine vétérinaire of the université de montréal for postmortem examination. aspiration pneumonia was the probable cause of death. necropsy revealed a focal papilloma-like penile lesion, characterized by focal mucosal thickening with disorganization of the epithelial layers and lymphopl ... | 2015 | 25375944 |
| sequence analysis of the 5' third of glycoprotein c gene of south american bovine herpesviruses 1 and 5. | bovine herpesviruses 1 (bohv-1) and 5 (bohv-5) share high genetic and antigenic similarities, but exhibit marked differences in tissue tropism and neurovirulence. the amino-terminal region of glycoprotein c (gc), which is markedly different in each of the viruses, is involved in virus binding to cellular receptors and in interactions with the immune system. this study investigated the genetic and antigenic differences of the 5' region of the gc (5' gc) gene (amino-terminal) of south american boh ... | 2015 | 25760029 |
| virus, strain, and epitope specificities of neutralizing bovine monoclonal antibodies to bovine herpesvirus 1 glycoproteins gb, gc, and gd, with sequence and molecular model analysis. | three bovine monoclonal antibodies (bomab) raised to bovine herpesvirus (bohv) 1.1 and specific for the viral glycoproteins gb, gc, and gd were tested for reactivity to two isolates of bohv-1.1, one of bohv-1.2, and two of bohv-5 in virus neutralization and indirect fluorescent antibody assays. they were also tested with other herpesviruses infecting cattle and other mammalian alphaherpesviruses, and found negative or of negligible reactivity. their bohv-1.1 epitope specificity was examined usin ... | 2015 | 25771882 |
| ntpdase and 5'-nucleotidase activities in synaptosomes of rabbits experimentally infected with bohv-5. | bovine herpesvirus type 5 (bohv-5) is the causative agent of herpetic meningoencephalitis in cattle. the purinergic system is described as a modulator of the immune response and neuroinflammation. these functions are related to the extracellular nucleotides concentration. ntpdase and 5'-nucleotidase are enzymes responsible for controlling the extracellular concentration of adenosine triphosphate (atp), adenosine diphosphate (adp), adenosine monophosphate (amp), and adenosine (ado). the aim of th ... | 2015 | 26025330 |
| outbreak control and clinical, pathological, and epidemiological aspects and molecular characterization of a bovine herpesvirus type 5 on a feedlot farm in são paulo state. | this paper describes the control, epidemiological, pathological, and molecular aspects of an outbreak of meningoencephalitis in calves due to bovine herpesvirus 5 at a feedlot with 540 animals in são paulo state, brazil. the introduction of new animals and contact between the resident animals and the introduced ones were most likely responsible for virus transmission. bovine herpesvirus 1 vaccine was used, resulting in the efficacy of the outbreak control, although two bovine herpesvirus 1 posit ... | 2015 | 26090469 |
| epidemiology and genetic characterization of bvdv, bhv-1, bhv-4, bhv-5 and brucella spp. infections in cattle in turkey. | the aim of the study was to determine the epidemiological data of bovine viral diarrhea virus (bvdv), bovine herpesvirus-1 (bhv-1), bovine herpesvirus-4 (bhv-4), bovine herpesvirus-5 (bhv-5) and brucella-associated cattle that were previously reported to have abortion and infertility problems in ankara, corum, kirikkale and yozgat provinces, turkey. whole blood and sera samples were obtained from 656 cattle, and antibodies against brucella spp. were detected in 45 (6.86%) and 41 (6.25%) animals ... | 2015 | 26096964 |
| development of a pyrosequencing assay for the typing of alphaherpesviruses. | identification of herpesvirus in biological material is usually carried out by real-time pcr. with the aim to classify the strain of virus identified, real-time pcr must be often supported by time-consuming capillary electrophoresis sequencing analysis. here we provide a protocol for the rapid and reliable identification of 5 closely related herpesviruses by pyromark q24 sequencing system. pyromark performs dna sequencing analysis using pyrosequencing, a technology based on the detection of rele ... | 2015 | 26150971 |
| comparative analysis of the replication of bovine herpesvirus 1 (bhv1) and bhv5 in bovine-derived neuron-like cells. | members of the subfamily alphaherpesvirinae use the epithelium of the upper respiratory and/or genital tract as preferential sites for primary replication. however, bovine herpesvirus 5 (bohv5) is neurotropic and neuroinvasive and responsible for meningoencephalitis in cattle and in animal models. a related virus, bohv1 has also been occasionally implicated in natural cases of neurological infection and disease in cattle. the aim of the present study was to assess the in vitro effects of bohv1 a ... | 2015 | 26239341 |
| a new method for simultaneous detection and discrimination of bovine herpesvirus types 1 (bohv-1) and 5 (bohv-5) using real time pcr with high resolution melting (hrm) analysis. | bovine herpesvirus types 1 (bohv-1) and 5 (bohv-5) are antigenically and genetically similar. the aim of this study was to develop a simple and reliable one-step real time pcr assay with high resolution melting (hrm) analysis for the simultaneous detection and differentiation of bohv-1 and bohv-5. optimization of assay conditions was performed with dna from reference strains. then, dna from field isolates, clinical samples and tissue samples of experimentally infected animals were studied by rea ... | 2016 | 26478539 |
| role of bovine herpesvirus type 5 (bohv-5) in diseases of cattle. recent findings on bohv-5 association with genital disease. | bovine herpesvirus type 5 (bohv-5) belongs to the family herpesviridae, subfamily alphaherpesvirinae, genus varicellovirus. this virus is a major causative agent of non-suppurative meningoencephalitis in young cattle. it was first isolated in 1962 from a neurological disease outbreak in australia. bohv-5 is genetically and antigenically related to bovine herpesvirus type 1 (bohv-1), a highly prevalent virus responsible for respiratory and genital disease in cattle. initially, bohv-5 was consider ... | 2012 | 26623291 |
| search for the genome of bovine herpesvirus types 1, 4 and 5 in bovine semen. | bovine herpesvirus type 1 (bohv-1) causes respiratory and reproductive disorders in cattle. recently, bovine herpesvirus type 5 (bohv-5) and bovine herpesvirus type 4 (bohv-4) have been identified to be associated with genital disease. in this study, the presence of the genome of bohv-1, bohv-4 and bohv-5 in bovine semen of argentinean and international origin was analyzed by pcr assays. the most important finding of this study is the detection of the genome of bohv-1 and bohv-4 in semen of bull ... | 2013 | 26623325 |
| development of an indirect elisa for serological diagnosis of bovine herpesvirus 5. | bovine herpesviruses 1 and 5 (bohv-1 and bohv-5) are economically important pathogens, associated with a variety of clinical syndromes, including respiratory and genital disease, reproductive failure and meningoencephalitis. the standard serological assay to diagnose bohv-1 and bohv-5 infections is the virus neutralization test (vnt), a time consuming procedure that requires manipulation of infectious virus. in the present study a highly sensitive and specific single dilution indirect elisa was ... | 2016 | 26866923 |
| comparison between dna detection in trigeminal nerve ganglia and serology to detect cattle infected with bovine herpesviruses types 1 and 5. | bovine herpesviruses (bohvs) types 1 (bohv-1) and 5 (bohv-5) are alphaherpesviruses of major importance to the bovine production chain. such viruses are capable of establishing latent infections in neuronal tissues. infected animals tend to develop a serological response to infection; however, such response-usually investigated by antibody assays in serum-may eventually not be detected in laboratory assays. nevertheless, serological tests such as virus neutralization (vn) and various enzyme-link ... | 2016 | 27224314 |
| bovine herpesviruses induce different cell death forms in neuronal and glial-derived tumor cell cultures. | oncolytic viruses have the ability to infect tumor cells and leave healthy cells intact. in this study, bovine herpesvirus 1 (bhv1; los angeles, cooper, and sv56/90 strains) and bovine herpesvirus 5 (bhv5; sv507/99 and gu9457818 strains) were used to infect two neuronal tumor cell lineages: neuro2a (mouse neuroblastoma cells) and c6 (rat glial cells). bhv1 and bhv5 strains infected both cell lines and positively correlated with viral antigen detection (p < 0.005). when neuro2a cells were infecte ... | 2016 | 27311457 |
| role of the suppressor of cytokine signaling 2 (socs2) during meningoencephalitis caused by bovine herpesvirus 5 (bohv-5). | the role of suppressors of cytokine signaling (socs) in meningoencephalitis caused by bovine herpesvirus 5 (bohv-5) was evaluated by intracranial infection in c57bl/6 wild-type mice (wt) and socs2 deficient mice (socs2(-/-)). both infected groups presented weight loss, ruffled fur and hunched posture. additionally, infected socs2(-/-) mice showed swollen chamfer and progressive depression. infected wt animals developed mild meningitis, characterized by infiltration of mononuclear cells. moreover ... | 2016 | 27477504 |
| the latency related gene of bovine herpesvirus types 1 and 5 and its modulation of cellular processes. | bovine herpesvirus type 1 (bohv-1) and bovine herpesvirus type 5 (bohv-5) are important pathogens of cattle. the diseases they produce are quite different, with bohv-5 being more neuropathogenic than bohv-1 which mainly induces respiratory symptoms. the sequencing of the entire bohv-5 genome has shown that most of the differences between these viruses are found in the immediate early and lr (latency related) genes. the lr gene is the only viral gene abundantly expressed in latently infected neur ... | 2016 | 27659677 |
| cellular response markers and cytokine gene expression in the central nervous system of cattle naturally infected with bovine herpesvirus 5. | the present study reports an investigation on the phenotype of inflammatory and immune cells, cytokine and viral gene expression in the brains of cattle naturally infected with bovine herpesvirus 5 (bhv5). brain sections of 38 affected animals were analysed for the nature and extent of perivascular cuffs in the virchow-robin space and parenchyma. histopathological changes were severe in the olfactory bulbs (obs), hippocampus, piriform, frontal, temporal and parietal cortices/lobes and were chara ... | 2016 | 27938713 |
| genome sequence of bubaline alphaherpesvirus 1 (buhv1) isolated in australia in 1972. | bubaline alphaherpesvirus 1 (buhv1) is a member of the family herpesviridae, subfamily alphaherpesvirinae, genus varicellovirus. to date, no full genome sequence of buhv has been published. here, we report the complete genome sequence of bubaline alphaherpesvirus 1 (buhv1) strain b6 (buhv1-b6), isolated from a water buffalo (bubalus bubalis) in 1972 in australia. the virus was multiplied in mdbk cells, and the dna was extracted and subjected to high-throughput sequencing. the reads were aligned ... | 2017 | 28063080 |
| prevalence of bovine herpesvirus type 4 in aborting dairy cows. | bovine herpesvirus type 4 (bhv-4) is related to many different conditions: infertility, postpartal metritis, vulvovaginitis, mastitis, encephalitis, calf pneumonia, keratoconjunctivitis, cutaneous lesions, digital dermatitis and abortion. in this study a retrospective pcr examination of 100 extracted dna samples from aborting cows was performed in order to determine: prevalence of bhv-4 in abortive cattle, whether coinfections bhv-4 with other abortifacient pathogens are present in the same samp ... | 2016 | 28092611 |
| expression of mir-155 associated with toll-like receptors 3, 7, and 9 transcription in the olfactory bulbs of cattle naturally infected with bhv5. | bovine herpesvirus 5 (bhv5) infection of young cattle is frequently associated with fatal neurological disease and, as such, represents an attractive model for studying the pathogenesis of viral-induced meningoencephalitis. following replication in the nasal mucosa, bhv5 invades the central nervous system (cns) mainly through the olfactory pathway. the innate immune response triggered by the host face to virus replication through the olfactory route is poorly understood. recently, an upregulatio ... | 2017 | 28831740 |
| susceptibility of mice to bovine herpesvirus type 5 infection in the central nervous system. | bovine herpesvirus type 5 (bohv-5) is an important pathogen that causes meningoencephalitis in cattle. few studies have used the mouse as a model for bohv-5 infection. despite the fact that bohv-5 can infect mice with immune deficiencies, little is known about viral replication, immune response, and the course of infection in the central nervous system (cns) of wild-type mice. therefore, the aim of this study was to evaluate the response in the cns of balb/c mice acutely infected with bohv-5 at ... | 2017 | 28942490 |
| evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5. | closely related bovine alphaherpesviruses 1 (bohv-1) and 5 (bohv-5) co-circulate in certain countries, rendering cattle co-infection possible. this is a prerequisite for bohv recombination. here, we report the first identification of homologous recombination between field isolates of bohv-1 and bohv-5, two alphaherpesviruses belonging to two distinct species with an average genomic similarity of 82.3%. three isolates of bohv-5, previously classified as subtype "bohv-5b", were phylogenetically st ... | 2017 | 28963062 |
| bacillus toyonensis improves immune response in the mice vaccinated with recombinant antigen of bovine herpesvirus type 5. | probiotics modulate the immune response and can increase the effectiveness of vaccines. bacillus toyonensis is widely used as a probiotic in animal feed. the aim of this study was to assess the effects of b. toyonensis administration on the immune response to an experimental recombinant vaccine against bovine herpesvirus type 5 (bohv-5) in mice. mice were vaccinated with bohv-5 recombinant glycoprotein d and supplemented with the probiotic b. toyonensis in two regimes: one group received the pro ... | 2018 | 29022386 |
| inhibition of apoptosis in bhv-1-infected cells depends on us3 serine/threonine kinase and its enzymatic activity. | us3 protein is a serine/threonine kinase conserved within the alphaherpesvirinae subfamily of herpesviruses. the us3 homologs of herpes simplex virus, pseudorabies virus, and bovine herpesvirus type 5 have been shown to block apoptosis triggered by viral infection or exogenous inducers. to determine whether these characteristics are shared by bovine herpesvirus type 1 us3, we constructed two viral mutants: bhv-1 us3 deletion mutant (bhv-1δus3) and a kinase-dead mutant (bhv-1kd). flow cytometry a ... | 2018 | 29073463 |
| probiotics bacillus toyonensis and saccharomyces boulardii improve the vaccine immune response to bovine herpesvirus type 5 in sheep. | there have been significant efforts toward the development of more efficient vaccines for animal health. a strategy that may be used to improve vaccine efficacy is the use of probiotics. bovine herpesvirus 5 (bohv-5) is an example of an important animal pathogen for which vaccines have provided only limited protection. in this study, we examined the use of the probiotics bacillus toyonensis and saccharomyces boulardii as a potential immune modulator to improve vaccine efficiency. thirty, 5-month ... | 2017 | 29331687 |
| neurotropic bovine astrovirus-associated encephalitis: an underdiagnosed disease in south america? | we describe a case of neurotropic bovine astrovirus-associated encephalitis in a jersey dairy cow from the department of san josé, uruguay. this represents the second case of this condition reported in the southern hemisphere. the cow was the only one affected in a herd of 70 cows, showing neurological signs with a 2-day clinical course, before dying spontaneously. histopathological examination revealed lymphocytic, histiocytic, and plasmacytic meningoencephalitis with neuronal necrosis, without ... | 2021 | 34148730 |