Publications

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a core set of microsatellite markers for conservation genetics studies of korean goral (naemorhedus caudatus) and its cross-species amplification in caprinae species.in order to screen microsatellites for conservation genetics studies of the species, a total of 23 microsatellite loci from korean goral (naemorhedus caudatus), including 15 previously developed loci and 8 new loci in this study, were tested. eleven microsatellites were screened and subjected to cross-species amplification using a test panel of four caprinae species, japanese serows (capricornis crispus), chinese gorals (naemorhedus goral), northern chamois (rupicapra rupicapra) and domestic goa ...201021113106
experimental infection of lambs with border disease virus isolated from a pyrenean chamois. 201021257444
spatial distribution and risk factors of brucellosis in iberian wild ungulates.the role of wildlife as a brucellosis reservoir for humans and domestic livestock remains to be properly established. the aim of this work was to determine the aetiology, apparent prevalence, spatial distribution and risk factors for brucellosis transmission in several iberian wild ungulates.201020205703
some pathological features of lungs from domestic and wild ruminants with single and mixed protostrongylid infections.lungs of 40 ruminants from bulgaria with natural small lungworm (nematoda: protostrongylidae) infections were investigated, including 16 goats, 15 sheep, 7 mouflons, and 2 chamois. muellerius capillaris, m. tenuispiculatus, cystocaulus ocreatus, neostrongylus linearis, and protostrongylus brevispiculum infections were predominantly associated with nodular lesions, and protostrongylus rufescens, protostrongylus hobmaieri and protostrongylus rupicaprae were associated with extensive lesions locate ...201020445790
complete genomic sequence of a border disease virus isolated from pyrenean chamois.this report describes the full-length genome sequence of the pestivirus strain h2121 which was recently isolated from pyrenean chamois and typed as border disease virus (bdv) genotype 4. comparison with full-length genomic sequences of the approved pestivirus species bovine viral diarrhea virus-1 (bvdv-1), bvdv-2, bdv, and classical swine fever virus, the tentative species represented by strain giraffe-1, as well as the atypical pestivirus strain th/04_khonkaen confirmed that the chamois pestivi ...201020638945
experimental infection of pigs with border disease virus isolated from pyrenean chamois (rupicapra pyrenaica).between 2001 and 2007, several outbreaks of disease associated with border disease virus (bdv) infection were reported in the central pyrenees (northeast spain) and were associated with a major reduction in chamois (rupicapra pyrenaica) populations. at the same time, wild boars (sus scrofa) from the same area were found to be seropositive to this pestivirus, without showing clinical signs. the present study examines the susceptibility of domestic swine and the course of the infection with a bdv ...201020453208
effects of early horn growth on reproduction and hunting mortality in female chamois.1. environmental conditions during early development can affect the growth patterns of vertebrates, influencing future survival and reproduction. in long-lived mammals, females that experience poor environmental conditions early in life may delay primiparity. in female bovids, annual horn growth increments may provide a record of age-specific reproduction and body growth. horn length, however, may also be a criterion used by hunters in selecting animals to harvest, possibly leading to artificial ...201021073455
polycystic liver disease in senile chamois (rupicaprae rupicaprae).a polycystic disease of the liver was diagnosed in 12 adult, free-living chamois (rupicaprae rupicaprae) from alpine regions in the western part of austria. the animals (eight female, four male), all in advanced age (average 13 yr old), were killed by local hunters, most because of emaciation or abnormal behavior. the livers of the animals were enlarged and contained multiple, thin-walled cysts of various sizes filled with serous, amber fluid. no evidence for parasites was found in the cysts. th ...201020688671
integrating phylogeographic patterns of microsatellite and mtdna divergence to infer the evolutionary history of chamois (genus rupicapra).the chamois, distributed over most of the medium to high altitude mountain ranges of southern eurasia, provides an excellent model for exploring the effects of historical and evolutionary events on diversification. populations have been grouped into two species, rupicapra pyrenaica from southwestern europe and r. rupicapra from eastern europe. however, a previous study of cytochrome b revealed that the two proposed species were non-monophyletic. the reconstruction of phylogenetic relationships b ...201020649956
characterization of dictyocaulus species (nematoda: trichostrongyloidea) from three species of wild ruminants in northwestern spain.specimens of dictyocaulus spp. were extracted from the respiratory tracts of 3 ruminant hosts including roe deer (capreolus capreolus), red deer (cervus elaphus), and chamois (rupicapra rupicapra) from wild populations in the province of le6n, northwestern spain. the near-complete nuclear small-subunit ribosomal rna gene, and 2 regions of the large-subunit ribosomal rna gene, were amplified by pcr and sequenced. the ssu rdna gene sequences indicated a high level of similarity between the isolate ...200920050000
investigation of the role of austrian ruminant wildlife in the epidemiology of malignant catarrhal fever viruses.malignant catarrhal fever (mcf) is an ubiquitous disease of cattle and other ruminants caused by ovine herpesvirus 2 (ovhv-2), which is endemic in sheep and transmitted from healthy carriers. further viruses of the mcf group are also able to induce mcf in ruminants. as alpine pasturing is very common in austria, possible contact with ruminant wildlife carrying and excreting mcf viruses might be suspected as an infection source. to investigate the epidemiologic role of austrian deer and chamois, ...200919395762
systemic toxoplasmosis and gram-negative sepsis in a southern chamois (rupicapra pyrenaica) from the pyrenees in northeast spain.a 6-year-old, male southern chamois (rupicapra pyrenaica) had an absence of flight response and was captured by hand in the catalan pyrenees in northeast spain. on clinical examination, the animal was in good body condition, and only atrophy of the right eye was observed. blood samples were collected and hematologic analysis performed, but no alterations were observed. the animal was sent to a wildlife rescue centre, where it developed chronic wasting and died after 32 days in captivity. at necr ...200919286506
nocardia otitidiscaviarum pneumonia in an alpine chamois (rupicapra rupicapra rupicapra).nocardia otitidiscaviarum was cultured from the lung of an alpine chamois (rupicapra rupicapra rupicapra) with suppurative bronchopneumonia. this is the first report of both nocardiosis and nocardia otitidiscaviarum in this wild ungulate species.200919361807
analysing the isotopic life history of the alpine ungulates capra ibex and rupicapra rupicapra rupicapra through their horns.the horn of ungulate grazers offers a valuable isotopic record of their diet and environment. however, there have been no reports of the spatio-temporal variation of the isotopic composition of horns. we investigated patterns of carbon (delta(13)c) and nitrogen (delta(15)n) isotopic composition along and perpendicular to the horn axis in capra ibex and rupicapra rupicapra rupicapra to assess the effects of animal age, within-year (seasonal) and inter-annual variation, natural contamination and s ...200919575404
babesia capreoli infections in alpine chamois (rupicapra r. rupicapra), roe deer (capreolus c. capreolus) and red deer (cervus elaphus) from switzerland.five cases of fatal babesiosis in free-ranging chamois (rupicapra r. rupicapra) attributed to infections with babesia capreoli were recently recorded in two regions of the swiss alps. to investigate the ecologic factors that possibly lead to those fatal b. capreoli infections in chamois, blood, ticks, and demographic data of 46 roe deer (capreolus c. capreolus), 48 chamois, and nine red deer (cervus elaphus) were collected in 2006 and 2007 in both affected regions. whereas no parasitic inclusion ...200919617485
even-toed but uneven in length: the digits of artiodactyls.in captive ruminants housed in small enclosures, hypertrophy of the outer hooves of the hindlimbs is often observed. we hypothesised that the underlying cause is overload attributable to an asymmetry of the digits, especially with respect to their length. to test this hypothesis, the bones of the digits of four species of artiodactyls, which included 11 wild chamois (rupicapra rupicapra), 11 captive fallow deer (dama dama), 11 captive bison (bison bison) and 11 european moose (alces alces; 9 wil ...200919386479
relationship between mtnr1a melatonin receptor gene polymorphism and seasonal reproduction in different goat breeds.the reproductive activity of goats bred in temperate latitude follows a seasonal pattern, influenced by annual variation in day length. daily variation in pineal melatonin secretion is the neuroendocrine signal recognized by animals through the link between this hormone and melatonin receptor 1a (mtnr1a). a total of 345 goats of different breeds (225 sarda, 30 saanen, 30 chamois coloured, 30 maltese and 30 nubian) with a kidding period in october-december or january-march were analysed to verify ...200918243602
detection of mycoplasma conjunctivae in the eyes of healthy, free-ranging alpine ibex: possible involvement of alpine ibex as carriers for the main causing agent of infectious keratoconjunctivitis in wild caprinae.mycoplasma conjunctivae is considered the major cause of infectious keratoconjunctivitis (ikc) in alpine ibex (capra i. ibex) and chamois (rupicapra r. rupicapra). while it is known that domestic sheep can act as healthy carriers for m. conjunctivae, this question has not been addressed in wild ungulates so far. in this study, bacteriological investigations and field observations were performed to assess whether free-ranging alpine ibex can be healthy carriers of m. conjunctivae. among 136 ibex ...200918834681
high prevalence of antibodies against chlamydiaceae and chlamydophila abortus in wild ungulates using two "in house" blocking-elisa tests.few data are available on the prevalence and relevance of chlamydiae in wild mammals, and even fewer studies have been conducted to determine the prevalence of chlamydophila abortus in wildlife hosts, most probably due to the absence of suitable species-specific serological assays for testing sera from wild animals. in light of this, we have developed two in-house blocking-elisa tests for detection of antibodies against chlamydiaceae and c. abortus in wild ungulates, and analyzed the relationshi ...200919010612
serologic and virologic investigations into pestivirus infection in wild and domestic ruminants in the pyrenees (ne spain).an outbreak of disease associated to a border disease virus was described in the southern chamois (rupicapra pyrenaica) in spain in 2002. sera and/or spleen samples from 57 mouflon, 15 red deer, 21 roe deer, 3 fallow deer, 55 sheep, 32 cattle, and 68 goats sharing the chamois habitat were studied. an antibody elisa test yielded an inconclusive result in 2 mouflon and positive results in 5 goat sera. comparative virus neutralization tests were performed on the 2 inconclusive mouflons, 3 of the 5 ...200919084245
first report of mycoplasma conjunctivae from wild caprinae with infectious keratoconjunctivitis in the pyrenees (ne spain).frequent outbreaks of infectious keratoconjunctivitis have been reported in wild caprinae in europe. while etiologic studies in the alps indicate that the main etiologic agent is mycoplasma conjunctivae, there are few reports from other mountain areas, such as the pyrenees, where m. conjunctivae has never been reported. in 2006 and 2007, five adult pyrenean chamois (rupicapra pyrenaica; two males and three females) and one adult male european mouflon (ovis orientalis musimon) were studied; they ...200919204357
border disease virus among chamois, spain.approximately 3,000 pyrenean chamois (rupicapra pyrenaica pyrenaica) died in northeastern spain during 2005-2007. border disease virus infection was identified by reverse transcription-pcr and sequencing analysis. these results implicate this virus as the primary cause of death, similar to findings in the previous epizootic in 2001.200919239761
genetic variability of the prion protein gene (prnp) in wild ruminants from italy and scotland.the genetics of the prion protein gene (prnp) play a crucial role in determining the relative susceptibility to transmissible spongiform encephalopathies (tses) in several mammalian species. to determine the prnp gene variability in european red deer (cervus elaphus), roe deer (capreolus capreolus) and chamois (rupicapra rupicapra), the prnp open reading frame from 715 samples was analysed to reveal a total of ten single nucleotide polymorphisms (snps). in red deer, snps were found in codons 15, ...200919461206
the mycoplasma conjunctivae genome sequencing, annotation and analysis.the mollicute mycoplasma conjunctivae is the etiological agent leading to infectious keratoconjunctivitis (ikc) in domestic sheep and wild caprinae. although this pathogen is relatively benign for domestic animals treated by antibiotics, it can lead wild animals to blindness and death. this is a major cause of death in the protected species in the alps (e.g., capra ibex, rupicapra rupicapra).200919534756
the genetic impact of translocations and habitat fragmentation in chamois (rupicapra) spp.the chamois is a useful species with which to investigate the combined genetic impact of habitat fragmentation, over hunting, and translocations. genetic variation within and between chamois (genus rupicapra) populations was analyzed in 259 individuals from 16 sampling sites located in italy, spain, slovakia, and the czech republic. two mitochondrial dna markers (control region and cytochrome b) and 11 nuclear microsatellites were typed. the principal results of this study can be summarized as f ...200919617524
cytochrome b phylogeography of chamois (rupicapra spp.). population contractions, expansions and hybridizations governed the diversification of the genus.the chamois provides an excellent model for exploring the effect of historical and evolutionary events on diversification. we investigate cytochrome b (cytb) sequences in the 10 recognized subspecies of rupicapra classified within 2 species: rupicapra pyrenaica, with the subspecies parva, pyrenaica, and ornata, and rupicapra rupicapra, with cartusiana, rupicapra, tatrica, carpatica, balcanica, asiatica, and caucasica. a fragment of 349 bp of the cytb was sequenced in 189 individuals. we identifi ...200818796461
immobilising free-ranging alpine chamois with xylazine, reversed with atipamezole.between 1996 and 2005, 215 free-ranging alpine chamois (rupicapra rupicapra) were immobilised with xylazine hydrochloride. the 110 male and 105 female animals received a mean (sd) dose of 2.5 (0.6) mg/kg with a range from 1.4 to 4.8 mg/kg. the immobilisation was reversed in 201 of the animals with an intramuscular injection of 0.3 (0.1) mg/kg atipamezole (range 0.03 to 0.76 mg/kg), corresponding to a mean ratio of atipamezole:xylazine of 1:9.4 (4.3). all the chamois were immobilised, but shorter ...200818689780
the factorial decomposition of the mahalanobis distances in habitat selection studies.the mahalanobis distances have been introduced in habitat selection studies for the estimation of environmental suitability maps (esms). the pixels of raster maps of a given area correspond to points in the multidimensional space defined by the mapped environmental variables (ecological space). the mahalanobis distances measure the distances in this space between these points and the mean of the ecological niche (i.e., the hypothesized optimum for the species) regarding the structure of the nich ...200818409444
disentangling the effects of recombination, selection, and demography on the genetic variation at a major histocompatibility complex class ii gene in the alpine chamois.the major histocompatibility complex (mhc) harbours some of the most polymorphic loci in vertebrate genomes. mhc genes are thought to be subject to some form of balancing selection, most likely pathogen-mediated selection. hence, mhc genes are excellent candidates for exploring adaptive processes. in this study, we investigated the genetic variation at exon 2 of the drb class ii mhc locus in 191 alpine chamois (rupicapra rupicapra) from 10 populations in the eastern alps of italy. in particular, ...200819238706
real-time pcr for quantitative detection of chamois (rupicapra rupicapra) and pyrenean ibex (capra pyrenaica) in meat mixtures.a real-time quantitative polymerase chain reaction (pcr) technique was developed for the quantification of chamois and pyrenean ibex dnas in meat mixtures by using a sybr green detection platform. two species-specific systems and a eukaryotic endogenous system were combined in the real-time pcr approach to quantify the target species. in the specific systems, a 133 base pair (bp) fragment of the 12s rrna gene was amplified from chamois dna, and an 88 bp fragment from the d-loop region was amplif ...200818376592
babesia divergens-like organisms from free-ranging chamois (rupicapra r. rupicapra) and roe deer (capreolus c. capreolus) are distinct from b. divergens of cattle origin - an epidemiological and molecular genetic investigation.in 2005 and 2006, three adult female chamois (rupicapra r. rupicapra) were found dead with signs of acute babesial infection in the eastern swiss alps. pcr on dna extracted from blood or spleen of the carcasses revealed sequence identity of the amplified part of the 18s rrna gene with genbank entries attributed to babesia divergens of cattle origin or b. capreoli of wild ruminant origin which have never been described before in this region. examination of 424 blood samples from 314 head of cattl ...200818400402
sarcoptic mange in red deer from spain: improved surveillance or disease emergence?concern about emerging diseases has risen in recent years, and multihost situations have become increasingly relevant for wildlife management and conservation. we present data on asturias, northern spain, where 80 mangy red deer (cervus elaphus) have been found since the beginning of the epizootic in chamois (rupicapra pyrenaica parva) in 1993. we combine field and necropsy data with the results of a serosurvey using an in-house elisa test to evaluate if deer mange due to sarcoptes scabiei is an ...200818430519
epidemiological study of border disease virus infection in southern chamois (rupicapra pyrenaica) after an outbreak of disease in the pyrenees (ne spain).in 2001 and 2002, an outbreak of a previously unreported disease, associated with a border disease virus (bdv), caused high mortality in the southern chamois (rupicapra pyrenaica) population in the alt pallars-aran national hunting reserve in the catalan pyrenees (ne spain). between 2002 and 2006, sera and/or tissue samples taken from 116 healthy chamois shot during the hunting season, plus 42 from chamois affected by different diseases, were studied. a blocking enzyme-immunosorbent assay (elisa ...200817881162
diseases and reproductive success in a wild mammal: example in the alpine chamois.density-dependent and climatic factors affect reproduction and dynamics of wild ungulates. parasites can also decrease reproductive success through either a direct abortive effect or a negative impact on host growth and body condition. however, few studies have investigated the effect of parasitism on fecundity of ungulates in natural conditions. we studied three bacterial infections caused by salmonella enterica serovar abortusovis, chlamydophila abortus and coxiella burnetii. these bacteria ar ...200818189146
authentication of meat from game and domestic species by snapshot minisequencing analysis.the aim of the present study is to develop an assay for the specific identification of meat from capreolus capreolus, cervus elaphus, capra ibex, rupicapra rupicapra, targeting sequences of the cytochrome b (cyt b) gene of mitochondrial dna. the assay is also intended to enable differentiation between meat from these wild species as well as ovis aries, capra hircus, bubalus bubalis, bos taurus and sus scrofa domestic species. the primers used in the preliminary pcr were designed in well conserve ...200822063325
pcr identification of meats from chamois (rupicapra rupicapra), pyrenean ibex (capra pyrenaica), and mouflon (ovis ammon) targeting specific sequences from the mitochondrial d-loop region.a polymerase chain reaction (pcr) assay was developed for the identification of meats from chamois (rupicapra rupicapra), pyrenean ibex (capra pyrenaica), and mouflon (ovis ammon) by using oligonucleotides targeting mitochondrial d-loop sequences. a d-loop region (~700-1000 bp) was firstly amplified and sequenced from various game and domestic meat dnas, and three primer sets were then designed on the basis of nucleotide multialignment of the generated d-loop sequences. as expected from sequence ...200722061240
molecular detection and characterization of piroplasms infecting cervids and chamois in northern spain.wildlife can act as reservoir of different tick-borne pathogens of veterinary and zoonotic importance. to investigate the role of wild ruminants as reservoir of piroplasm infection, 28 red deer, 69 roe deer and 38 chamois from northern spain were examined by reverse line blot (rlb) hybridization. the survey detected a prevalence of 85.7% in red deer, 62.3% in roe deer and 28.9% in chamois. four different piroplasms were identified: theileria sp. ot3 (previously described in sheep) as the most pr ...200717076924
transmission of a pestivirus infection in a population of pyrenean chamois.outbreaks of a previously unrecorded disease have recently affected pyrenean chamois (rupicapra pyrenaica pyrenaica) populations across the mountain range. a pestivirus was hypothesized to be the cause of this emerging disease and this type of virus can cross the species barrier and be transmitted to or from wildlife. using an epidemiological survey conducted from 1995 to 2004 at orlu, france, we characterized the virus and analyzed its transmission. a phylogenetic analysis of viral sequences an ...200717092662
severe outbreak of disease in the southern chamois (rupicapra pyrenaica) associated with border disease virus infection.an outbreak of a previously unreported disease affecting southern chamois (rupicapra pyrenaica) in the central pyrenees (ne spain) was recorded in 2001 and 2002. there was a marked temporal distribution, most animals being found between february and june. after the outbreak, the population was found to have decreased by about 42%, most probably due to the disease. we examined 20 affected chamois. clinical manifestations included depression, weakness and movement difficulties in all cases. three ...200717101242
genetic and antigenic typing of border disease virus isolates in sheep from the iberian peninsula.a selection of 10 pestiviruses isolated from sheep from the iberian peninsula from 2001 to 2004 was characterised at the molecular level. the 5' untranslated region (5'-utr) and n(pro)-coding gene were amplified by the reverse transcription-polymerase chain reaction (rt-pcr) and sequenced directly from purified products. all isolates were also typed antigenically with a panel of monoclonal antibodies (mabs) raised against representative isolates of the four recognised pestivirus species. the gen ...200717126573
analysis of mitochondrial dna for authentication of meats from chamois (rupicapra rupicapra), pyrenean ibex (capra pyrenaica), and mouflon (ovis ammon) by polymerase chain reaction-restriction fragment length polymorphism.the prevention of fraudulent labeling of game meat constitutes an important part of food regulatory control and quality assurance systems. a polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) analysis based on mitochondrial deoxyribonucleic acid (dna) was developed for authentication of meats from chamois (rupicapra rupicapra), pyrenean ibex (capra pyrenaica), and mouflon (ovis ammon). amplification and restriction site analysis of a dna fragment about 720 base pairs ( ...200717373450
diversity and evolution of the mhc-drb1 gene in the two endemic iberian subspecies of pyrenean chamois, rupicapra pyrenaica.major histocompatibility complex class ii locus drb variation was investigated by single-strand conformation polymorphism analysis and sequence analysis in the two subspecies of pyrenean chamois (rupicapra pyrenaica) endemic to the iberian peninsula. low levels of genetic variation were detected in both subspecies, with seven different alleles in r. p. pyrenaica and only three in the r. p. parva. after applying the rarefaction method to cope with the differences in sample size, the low allele nu ...200717551521
babesiosis in free-ranging chamois (rupicapra r. rupicapra) from switzerland.pathological examination of five adult chamois (rupicapra r. rupicapra) found dead in two different regions from the swiss alps revealed pale mucous membranes and musculature, swollen spleen and haemoglobinuria. histologically, haemosiderosis in the spleen and centrilobular hepatic necrosis were the predominant findings. on blood smears, small (approximately 0.84-1.47 microm), round to pyriform, peripherally located inclusions were present in the erythrocytes. pcr followed by sequencing of dna e ...200717689015
dermatophytosis caused by trichophyton mentagrophytes in the southern chamois (rupicapra pyrenaica) in the eastern pyrenees.two free-ranging southern chamois (rupicapra pyrenaica) - one 4-year-old male and one 3-year-old male - were diagnosed with clinical dermatophytosis in the french and spanish eastern pyrenees respectively. skin samples were collected for microbiological studies, trichophyton mentagrophytes being isolated and identified in both animals. the first chamois was found dead at the base of a cliff, and presented with alopecia and scaling on the dorsum and left forelimb. the second chamois showed grey-y ...200717803516
seroprevalence of neospora caninum in non-carnivorous wildlife from spain.serum samples from 1034 non-carnivorous wildlife from spain were tested for antibodies to neospora caninum by competitive screening enzyme linked immunosorbent assay (elisa) and confirmed by an indirect fluorescent antibody test (ifat). high agreement was observed between results in both techniques (kappa value higher than 0.9). prevalences of n. caninum antibodies positive by both techniques were 11.8% of 237 red deer (cervus elaphus), 7.7% of 13 barbary sheep (ammotragus lervia), 6.1% of 33 ro ...200716962706
identification and heterologous expression of a sarcoptes scabiei cdna encoding a structural antigen with immunodiagnostic potential.the mite sarcoptes scabiei causes sarcoptic mange (or scabies), a disease of considerable human and veterinary significance. an s. scabiei cdna clone of about 2 kb was isolated from a s. scabiei var. hominis expression library by immunological screening using blood serum from a naturally infected chamois (rupicapra rupicapra). the nucleotide sequence of the identified cdna contains an open reading frame of 1930 bp that encodes a 642 amino acid polypeptide. this polypeptide shows tandem repeats o ...200717506973
cytochrome b pseudogene originated from a highly divergent mitochondrial lineage in genus rupicapra.we have identified a nuclear pseudogene (numt) of cytochrome b (cytb) in chamois. the comparison of a fragment of 402 nucleotides of cytb and the pseudogene between the 2 species rupicapra rupicapra and rupicapra pyrenaica allowed direct measurement of relative rates and patterns of evolution. mitochondrial genes evolved 7 to 12 times faster than their nuclear counterparts. substitutions in the nucleus include a frameshift and a stop codon. phylogenetic analysis of nuclear and mitochondrial line ...200717416931
semen evaluation in the chamois of abruzzi (rupicapra pyrenaica ornata).the chamois of abruzzi (rupicapra pyrenaica ornata) has been classified as endangered by the world union for conservation. the objective of this study was to analyze seasonal differences in the characteristics of various male reproductive organs and in semen quality. the study was conduced during 2004 in the reserve of lama dei peligni (italy) on three chamois males aged between 2 and 5 years. males were captured during march-may months and october-december months. various testicular and scrotal ...200716540267
statistical correlations between the in-mouth textural characteristics and the chemical composition of shiraz wines.the relationships between the levels of polyphenols, acidity, and red pigments in shiraz wines and their perceived textural profiles as quantified by a trained sensory descriptive analysis panel were explored. a "chamois-like" feeling when the wine was held in the mouth appeared to be related to an absence of polyphenols. the in-mouth "chalk-like" texture was strongly associated with anthocyanin concentration and was negatively associated with alcohol level and acidity. the astringent subqualiti ...200717348678
[mesenchymal tumor in a female chamois. a case report].a free-living 15-year old female chamois was shot because of illness and emaciation. the examination of the internal organs revealed numerous firm whitish tumour nodules in liver,spleen, mesenterium and peritoneum. histologically the nature of tumour cells was identified as a metastasizing malignoma of mesenchymal origin. in addition to the blastoma, the parasite burden of the gastrointestinal tract and lungs did very likely contribute to the bad bodily condition of the chamois.200617009723
models of parasite biocoenosis dynamic: host density and gastrointestinal parasites in alpine chamois.host density is an important and widely accepted factor influencing microparasites epidemiology. in theory, host density would influence also macroparasite dynamic, although it would be achieved indirectly due to the presence of free-living infective stages of parasites. on this basis, it is expected that macroparasite abundance and prevalence would increase as host density increases, due to the higher probability for a new host to acquire infections from the environment. nevertheless, some surv ...200616881395
steroid hormone related male biased parasitism in chamois, rupicapra rupicapra rupicapra.parasites are linked with their host in a trophic interaction with implications for both hosts and parasites. interaction stretches from the host's immune response to the structuring of communities and the evolution of biodiversity. as in many species sex determines life history strategy, response to parasites may be sex-specific. males of vertebrate species tend to exhibit higher rates of parasites than females. sex-associated hormones may influence immunocompetence and are hypothesised to lead ...200616497439
transport stress in southern chamois (rupicapra pyrenaica) and its modulation by acepromazine.chamois have been translocated for more than 100 years but their stress response to transport remains to be elucidated. in this study, 21 free-ranging southern chamois were captured, physically restrained and then transported. the animals were randomly injected intramuscularly with acepromazine (nine adult males, one adult female and one yearling male) or saline (five adult males, two adult females and three yearling males). heart rate and body temperature were monitored with telemetric devices, ...200616154370
genetic typing and prevalence of border disease virus (bdv) in small ruminant flocks in spain.between 2001 and 2002, samples from 1,413 animals in 21 spanish small ruminant flocks, most of them with animals showing clinical signs compatible with border disease (bd), were screened for the presence of pestivirus antigen and antibodies by an indirect peroxidase monolayer assay (ipma) and the virus neutralization test (vnt), respectively. although all flocks harboured seropositive animals, virus could only be isolated from animals in five of the flocks. between 4 and 11 months later all anim ...200616857326
serosurvey of roe deer, chamois and domestic sheep in the central italian alps.roe deer (capreolus capreolus), chamois (rupicapra rupricapra rupicapra), and domestic sheep in the orobie alps, italy, were serologically tested for antibodies to selected pathogens that may be transmitted across species. antibodies against brucella spp. and bovine herpesvirus 1 (roe deer and chamois only) were not detected in any species. in roe deer, antibodies were detected against toxoplasma gondii (13%) and neospora caninum (3%). chamois tested positive for antibodies to t. gondii (5%), n. ...200617092903
contact rates and exposure to inter-species disease transmission in mountain ungulates.the risk for a pathogen to cross the species barrier depends on the rate of efficient contacts between the species. however, contact rates between species have rarely been estimated from observations. here we estimate contact rates and exposure of chamois rupicapra rupicapra and alpine ibex capra ibex exposed to domestic pasteurellosis and brucellosis carried by sheep or cattle herds summering in mountain pastures. we use field observation data on animal positions treated in a geographic informa ...200616409647
spiculopteragia mathevossiani ruchliadev, 1948 is the minor morph of spiculopteragia spiculoptera (gushanskaya, 1931): molecular evidence.although spiculopteragia spiculoptera is primarily a parasite of cervids, it can also but less often contaminate domestic livestock. little is known about its epidemiology and its pathogenicity in domestic ruminants and other unusual cervid species. its taxonomic status remains unclear since the hypothesis of morphological polymorphism among males has been proposed. however, accurate taxonomy is fundamental in the identification and survey of potentially pathogenic species of parasites. the seco ...200616777039
haematological and serum biochemical values of southern chamois (rupicapra pyrenaica).blood samples were taken from 75 free-ranging southern chamois (rupicapra pyrenaica) captured in drive-nets in catalonia, north-eastern spain, and 20 haematological and 24 serum biochemical variables were analysed. the values were similar to those of other species of the caprinae subfamily, except for cortisol, the concentration of which was higher. the red blood cell count (rbc), platelets and leucocytes, and the concentrations of cortisol, lactate, muscular enzymes and gamma-globulins were hig ...200616603553
abomasal nematode community in an alpine chamois (rupicapra r. rupicapra) population before and after a die-off.abomasa of 185 chamois shot during 5 consecutive hunting seasons were collected as part of a health monitoring program in an alpine area of italy and examined for nematodes. the data were obtained during both the preceding period and that following a severe die-off caused by a pneumonia outbreak. prevalence, mean abundance, mean intensity, and thul importance index were consistently high, in particular for haemonchus contortus, having a low host specificity and high pathogenic potential. species ...200617152929
prevalence of toxoplasma gondii antibodies in red deer (cervus elaphus) and other wild ruminants from spain.serum samples from 441 red deer (cervus elaphus) and 161 other wild ruminant species, collected between 1993 and 2005 from six regions of spain were tested for antibodies against toxoplasma gondii by the modified agglutination test (mat). antibodies to t. gondii (mat 1:25 or higher) were detected in 15.6% of red deer. statistically significant differences were observed among sampling sites with seroprevalence in red deer from catalonia (42.2%) being significantly higher compared with other spani ...200616359801
babesia spp. identified by pcr in ticks collected from domestic and wild ruminants in southern switzerland.concurrent infections with vector-borne pathogens affected a cattle herd in switzerland, and one of the pathogens was identified as babesia bigemina, which had never been observed in this country before. therefore, a survey of the occurrence of ruminant babesia spp. and their tick vectors in switzerland was conducted. a total of 2,017 ticks were collected from sheep, goats, cattle, and wild ruminants (deer, roe deer, and chamois) in southern parts of switzerland and identified morphologically. t ...200617021198
astrocytoma in a chamois.astrocytomas represent the most common cerebral tumors in humans and in animals, and the fibrillary cytological subtype is the most frequently observed. in this report and for the first time, a thalamic astrocytoma is described in a chamois showing depressed mentation, pleurothotonus and circling to the right side.200516259411
[mycobacterium avium subsp. paratuberculosis in wild animal species and cattle in styria/austria].infections with mycobacterium ovium ssp. paratuberculosis (m. paratuberculosis) are increasingly recognised worldwide. in addition to an increased prevalence of paratuberculosis in austrian cattle herds, recent years have also shown a rise in infections with m. paratuberculosis in wild red and roe deer, chamois and mouflon. during the period from june 2002 to september 2004, mesenteric lymph nodes were taken from a total of 483 wild animals hunted or found dead and from 338 deceased cattle. samp ...200516048043
[the incidence of bacterial cns infections in roe deer (capreolus capreolus), red deer (cervus elaphus) and chamois (rupicapra rupicapra) in bavaria].brain samples of 849 wild ruminants (654 roe deer, 189 red deer and 6 chamois) from bavaria were examined for the occurrence of encephalopathies caused by bacteria, using cultural, serological and genetic methods. in addition, 87 brain samples were investigated histologically for clarification of the pathogenetic relevance of specific microorganisms. using conventional bacteriological methods, 464 different bacteria were isolated. 229 of them could be differentiated to the genus level and 235 to ...200515690635
season effect on genitalia and epididymal sperm from iberian red deer, roe deer and cantabrian chamois.seasonality deeply affects the physiology and behavior of many species, and must be taken into account when biological resource banks (brbs) are established. we have studied the effect of seasonality on many reproductive parameters of free-ranging iberian red deer, roe deer and cantabrian chamois, living in spain. testicles from hunted animals were collected and sent to our laboratory at different times during the year. we recorded the weight and volume of testis, the weight of the epididymis an ...200515823344
seroprevalence of pestivirus in four species of alpine wild ungulates in the high valley of susa, italy.wildlife, once infected, can serve as a reservoir of infectious diseases that form a constant threat to domestic livestock. to make control and eradication programs successful in the long-term, presence of pestivirus in wildlife populations should be monitored. the goal of this study was to investigate seroprevalence of pestivirus in four alpine wild ungulates in the high valley of susa, north-west italy. species studied were: red deer (cervus elaphus), roe deer (capreolus capreolus), wild boar ...200515922522
molecular dating of caprines using ancient dna sequences of myotragus balearicus, an extinct endemic balearic mammal.myotragus balearicus was an endemic bovid from the balearic islands (western mediterranean) that became extinct around 6,000-4,000 years ago. the myotragus evolutionary lineage became isolated in the islands most probably at the end of the messinian crisis, when the desiccation of the mediterranean ended, in a geological date established at 5.35 mya. thus, the sequences of myotragus could be very valuable for calibrating the mammalian mitochondrial dna clock and, in particular, the tree of the c ...200516332256
outbreaks of infectious keratoconjunctivitis in alpine chamois and ibex in switzerland between 2001 and 2003.five outbreaks of infectious keratoconjunctivitis (ikc) affecting alpine chamois and ibex in the western and central swiss alps were recorded in 2001 to 2003. mycoplasma conjunctivae was identified from conjunctival swabs by means of a nested pcr in 27 of the 28 chamois tested. the outbreaks occurred in an area covering 1590 km(2). deep valleys acted as a barrier to the spread of the disease. a total of 409 chamois and 33 ibex with clinical signs of ikc were reported. most of the chamois were sh ...200515995237
cilia-associated respiratory (car) bacillus infection in adult red deer, chamois, and roe deer.cilia-associated respiratory (car) bacillus is an unclassified bacterium that colonizes the ciliated epithelium of airways in laboratory rats, laboratory mice, and laboratory and conventionally reared rabbits, cattle, goats, and pigs. data on the prevalence of car bacillus infection in wild animals are lacking. the present study demonstrated the occurrence of the organism in wild red deer (cervus elaphus hippelaphus), chamois (rupicapra rupicapra), and roe deer (capreolus capreolus) from the val ...200516107685
detection of a newly described pestivirus of pyrenean chamois (rupicapra pyrenaica pyrenaica) in france.a pestivirus was detected and characterized in chamois (rupicapra pyrenaica pyrenaica) originating from the french part of the pyrenees. phylogenetic analysis of the pestivirus was done on the basis of a fragment from the 5' noncoding region including 22 published nucleotide sequences of different pestivirus strains. our strain was grouped within the clade of border disease viruses (bdv). however, it had an intermediate position between clade bdv and classical swine fever viruses representing a ...200516244072
recombination and the origin of sequence diversity in the drb mhc class ii locus in chamois (rupicapra spp.).we examined the evolutionary processes contributing to genetic diversity at the major histocompatibility complex (mhc) class ii drb locus in chamois (rupicapra spp., subfamily caprinae). we characterised the pattern of intragenic recombination (or homologous gene conversion) and quantified the amount of recombination in the genealogical history of the two chamois species, pyrenean chamois (rupicapra pyrenaica) and alpine chamois (rupicapra rupicapra). we found evidence for intragenic recombinati ...200515756546
first report of white mold caused by sclerotinia sclerotiorum on hutchinsia alpina in italy.hutchinsia alpina (chamois cress), a plant belonging to the brassicaceae family, is cultivated as a potted ornamental plant in italy. in the winter of 2002, leaves and stems of plants grown in pots (14-cm diameter) in commercial farms near albenga (northern italy) developed soft and watery tissues covered with whitish mycelium at the soil line. as necrosis progressed, infected plants wilted and died. dark sclerotia developed on infected tissues. because of high planting density, the infection sp ...200430812223
a note on the identification of rupicapra rupicapra species by polymerase chain reaction product sequencing.to identify meat samples suspected of containing a protected game species (chamois-rupicapra rupicapra) polymerase chain reaction (pcr) product sequencing was performed. the amplicon was obtained with a pair of "universal" primers. the results confirm those of isoelectric focusing (ief) on the same samples.200422060886
outbreak of scabies in human beings, acquired from chamois (rupicapra rupicapra). 200415559427
pestivirus exposure in free-living and captive deer in austria.during the hunting season of 2001-02, blood and spleen samples from 59 red deer (cervus elaphus), 77 roe deer (capreolus capreolus), four fallow deer (dama dama), and five chamois (rupicapra rupicapra) were collected from nine hunting districts (n = 133) and one deer farm (n = 12) in southern austria. sera were tested for antibodies against bovine viral diarrhea virus (bvdv) with an enzyme-linked immunosorbent assay (elisa) and virus neutralization tests against three bvdvs and one border diseas ...200415650102
molecular identification of a new pestivirus associated with increased mortality in the pyrenean chamois (rupicapra pyrenaica pyrenaica) in spain.pestivirus infection was identified in 16 of 17 chamois during an outbreak of a previously unreported disease in pyrenean chamois (rupicapra pyrenaica pyrenaica) in northeastern spain in 2001-02. by analysis of the 5' noncoding regions of the virus, we assigned it to the border disease virus cluster with pairwise similarity values ranging from 82.1% to 88.1%. it will be important to investigate the association of this pestivirus with disease in pyrenean chamois.200415650103
a novel pestivirus associated with deaths in pyrenean chamois (rupicapra pyrenaica pyrenaica).during investigations into recent population decreases in pyrenean chamois (rupicapra pyrenaica pyrenaica) 21 animals found dead or dying were necropsied. immunohistochemistry revealed the presence of a pestivirus in organs from two of the 21 chamois. from one of these animals a pestivirus was isolated from the spleen, skin and serum. the virus had better growth in ovine than in bovine cells and was neutralized most effectively by an anti-border disease virus (bdv) reference antiserum. using pan ...200415557238
sequence analysis of the mhc class ii drb alleles in alpine chamois (rupicapra r. rupicapra).this study characterises the expressed mhc class ii drb gene and its genetic variation in exon 2 among 59 alpine chamois (rupicapra r. rupicapra) from several locations in the eastern alps plus one population in the western alps. the expressed drb gene contains all the expected characteristics including all of the conserved residues found in other mammalian species. the isolated expressed drb gene from chamois can be considered orthologous to mhc class ii drb genes in other mammalian species. ca ...200414642892
[the occurrence of transmissible spongiform encephalopathies in roe deer (capreolus capreolus), red deer (cervus elaphus) and chamois (rupicapra rupicapra) in bavaria].brain samples of 849 free-ranging ruminants (654 roe deer, 189 red deer and 6 chamois) from bavaria were examined for tse. in this respect, detections of prpres were carried out by elisa technique (biorad) and immunohistochemistry using the monoclonal antibody (mab) l42. validation experiments of the elisa technique for the detection of prionprotein from free-ranging ruminants showed that the examination of brains of wild ruminants lead to lower extinctions than that of bovine brain samples. due ...200414964120
biotin-avidin amplified elisa for detection of antibodies to sarcoptes scabiei in chamois (rupicapra spp.).scabies is a major threat to the well being of mountain-dwelling bovid hosts, rupicapra rupicapra and rupicapra pyrenaica. severe outbreaks are in progress over a significant part of their distribution area and resource managers demand improved methods to monitor, analyse and possibly forecast the spread and effects of scabies at the population level. an amplified capture enzyme-linked immunosorbent assay was developed to detect antibodies to sarcoptes scabiei in chamois (rupicapra spp.) serum. ...200415535959
spatial patterns of mitochondrial and nuclear gene pools in chamois (rupicapra r. rupicapra) from the eastern alps.we have assessed the variability of maternally (mtdna) and biparentally (allozymes) inherited genes of 443 chamois (rupicapra r. rupicapra) from 19 regional samples in the eastern alps, to estimate the degree and patterns of spatial gene pool differentiation, and their possible causes. based on a total mtdna-rflp approach with 16 hexanucleotide-recognizing restriction endonucleases, we found marked substructuring of the maternal gene pool into four phylogeographic groups. a hierarchical amova re ...200312886279
prevalence of antibodies to borrelia burgdorferi sensu lato in southern chamois (rupicapra pyrenaica) in spain.ungulates are involved in the epidemiology of borreliosis as maintenance hosts for vector tick species. we evaluated the prevalence of antibodies to borrelia burgdorferi sensu lato in two different populations of southern chamois in spain. forty-six sera (40.4%) were positive at titres >/=1 : 64 using an indirect immunofluorescence assay (ifat).200312864902
serological study of a population of alpine chamois (rupicapra r rupicapra) affected by an outbreak of respiratory disease.a serological survey of respiratory virus infections was carried out from 1998 to 2001 in lecco province, italy, as part of a health monitoring programme in a population of alpine chamois, many of which died of pneumonia in autumn and winter 2000 to 2001; 194 carcases of all age classes were found over a short period and in a small area. eighteen of them, which were examined postmortem, consistently showed signs of severe fibrinous lobar pneumonia or catarrhal bronchopneumonia. samples of serum ...200314640327
genetic regulation of border zone formation in female mastomys (praomys coucha) adrenal cortex.the unique border zone between the zona fasciculata and z. reticularis of the female adrenal cortex is formed in the wild-colored inbred mastomys (praomys coucha) strain, mwc, but never in the chamois-colored inbred strain, mcc. this clear strain-specific trait was genetically analyzed using f1, f2, and backcross progenies produced between mwc and mcc. reciprocal crosses gave no significant differences in the phenotypic ratio of f1 or f2 progeny. border zone formation was detected in 0% of f1 fe ...20038742820
[composition of the gastrointestinal nematode population in the chamois (rupicapra rupicapra l.) in relation to variations in the environment in some zones of the central alpine arc]. 20036544397
pcr detection and serological evidence of granulocytic ehrlichial infection in roe deer (capreolus capreolus) and chamois (rupicapra rupicapra).the role of wild mammals, such as roe deer (capreolus capreolus) and chamois (rupicapra rupicapra), in the epidemiology of granulocytic ehrlichiae in switzerland was investigated. we tested blood samples for ehrlichia phagocytophila genogroup 16s rrna gene sequences by pcr and for immunoglobulin g antibodies against granulocytic ehrlichiae by indirect fluorescent-antibody assay (ifa). overall means of 60.9% of 133 roe deer serum samples and 28.2% of 39 chamois serum samples were seroreactive by ...200211880411
ribosomal and mitochondrial dna sequence variation in sarcoptes mites from different hosts and geographical regions.in order to investigate the extent of the genetic variation in the dna sequences of sarcoptes scabiei, mite populations collected on alpine chamois ( rupicapra rupicapra), pyrenean chamois ( rupicapra pyrenaica) and red fox ( vulpes vulpes) from different localities of italy and spain were studied. sequence analyses were carried out on the second internal transcribed spacer of the nuclear ribosomal dna and on the 16s mitochondrial rrna gene. its-2 sequences showed a higher degree of genetic poly ...200212122437
a review of mutual transmission of important infectious diseases between livestock and wildlife in europe.oral vaccination of red foxes against rabies has been practiced in europe since 1978 and has succeeded in greatly reducing the occurrence of this disease in foxes: this is an example of coordinated activity against a disease that affects both wild and domestic animals as well as humans. some examples of diseases that affect both domestic and wild animals in europe are: classical swine fever (hog cholera) in wild boars and domestic swine; myxomatosis and rabbit hemorrhagic disease in domestic and ...200212381556
phylogeography of chamois (rupicapra spp.) inferred from microsatellites.evolutionary relationships among populations of chamois (rupicapra spp.) across their current range from the caucasus to the cantabrian mountains were investigated. the allelic variation in 23 microsatellite loci was assessed in eight geographical populations, recognised as subspecies of the two closely related species r. pyrenaica and r. rupicapra. analysis of variance in allele frequencies (fst, statistics) and in repeat numbers (rst, statistics) showed these data to be highly structured. two ...200212450756
infectious keratoconjunctivitis of ibex, chamois and other caprinae.infectious keratoconjunctivitis (ikc) caused by mycoplasma conjunctivae is a highly contagious ocular infection which is common in domestic sheep and goats. in the european alps, ikc is often observed in alpine chamois (rupicapra r. rupicapra) and in alpine ibex (capra i. ibex), but the disease has also been described in other wild caprinae in the pyrenees and in new zealand. the infection is characterised by inflammation of the conjunctiva and cornea, and in the most advanced stages, the cornea ...200211974619
dermatophilosis of alpine chamois (rupicapra rupicapra) in italy.a proliferative dermatitis similar to the condition generally referred to as strawberry footrot was observed in two alpine chamois (rupicapra rupicapra) from eastern alps, italy. branching septated filaments and packets of pas-positive coccoid organisms were observed in histological sections of the affected skin. the actinomycete, dermatophilus congolensis, was isolated from crusted lesions in one chamois. as wild ruminants are presumed to be a reservoir of infection in the alpine area, the auth ...200211980380
mycoplasma conjunctivae infection is not maintained in alpine chamois in eastern switzerland.the occurrence of infectious keratoconjunctivitis (ikc) was assessed in alpine chamois (rupicapra rupicapra rupicapra) in grisons (switzerland) from 1950 to 1999. the first ikc outbreaks were reported in the 1950's. since then, the number of affected subpopulations constantly increased and, by 1999, ikc outbreaks were reported in 39 of 51 (77%) chamois sub-populations. from 1992-99, a total of 243 chamois which died of the consequences of ikc were recorded. the number of cases differed between y ...200212038129
use of variograms to detect critical spatial distances for the knox's test.we aimed to find critical spatial distances (as an input quantity for the knox's test) using geostatistical methods. the spatiotemporal data set included all individual-chamois cases of scabies observed in styrian chamois populations in 137 of 2837 game preserves in styria (a province in the south of austria) over the time period between 1952 and 1998. theoretical variogram models were fitted to empirical variograms, which were calculated for cycles of 5 years. the unit of analysis was the mean ...200212062518
gastro-intestinal nematode infections in four alpine chamois herds: influence of host density on helminth egg output.a three years survey on gastro-intestinal nematode egg output was carried out in four alpine chamois herds (rupicapra rupicapra rupicapra) from belluno province (italy). data were analysed in order to detect the influence of cattle presence and chamois density on egg output, and to evaluate the stability of host-parasite relationship. as expected, variations related to the season were present, with the major peak of egg output observed in the summer. results highlighted an unexpected inverse inf ...200111921539
mitochondrial dna from myotragus balearicus, an extinct bovid from the balearic islands.dna was extracted from teeth and bones of myotragus balearicus, a bovid that evolved in isolation on the balearic islands (western mediterranean) from the end of the miocene, becoming extinct 4,000 years bp, after the arrival of humans in the islands. the numerous morphological apomorphies of myotragus, most strikingly its dwarfism, frontal eyes, and ever-growing incisors, obscure its phylogenetic relationships with extant bovids. therefore, the recovery of genetic information from myotragus is ...200010750053
immune responses to mycoplasma conjunctivae in alpine ibex, alpine chamois, and domestic sheep in switzerland.the humoral immune response of three alpine chamois (rupicapra rupicapra rupicapra), two alpine ibex (capra ibex ibex) and three domestic sheep naturally affected with infectious keratoconjunctivitis (ikc), and four ibex and two sheep experimentally infected with mycoplasma conjunctivae was analysed. in addition, the local immune response to m. conjunctivae was analysed using conjunctival washes from chamois and sheep. immunoblot analysis of sera using whole cell antigens of m. conjunctivae reve ...200010813608
effects of sarcoptic mange on serum proteins and immunoglobulin g levels in chamois (rupicapra pyrenaica) and spanish ibex (capra pyrenaica).three groups of chamois (rupicapra pyrenaica) and three groups of spanish ibex (capra pyrenaica) were established to study the effects of sarcoptic mange on serum proteins and immunoglobulin g (igg) levels. the first group of chamois consisted of 22 healthy pyrenean chamois (r. pyrenaica pyrenaica) from a non-infested area, the second group consisted of 20 healthy cantabrian chamois (r. p. parva) from an area where sarcoptic mange has been reported since 1994 and the third group consisted of 16 ...200010714470
lung-tissue extract as an alternative to serum for surveillance for brucellosis in chamois.serological surveys are the most-used way to study diseases in free-ranging wild animals. however, the difficulty in obtaining a sufficient number of suitable serum samples is a major problem. to resolve this problem, we investigated the possibility of using lung-tissue extract in place of blood serum for searching for antibodies against brucella abortus. antibodies titres against b. abortus was tested from blood serum and lung-tissue extract from 112 chamois and 99 cattle. although in complemen ...200010673058
report of a case of bronchopneumonia associated with moraxella bovis isolation in a chamois (rupicapra pyrenaica).a case of fibrinopurulent bronchopneumonia associated with moraxella bovis infection in a chamois (rupicapra pyrenaica) is described. the animal, a 4-month-old female, was referred by the staff warden of the national game reserve of freser-setcases (catalonia, north-eastern spain). the animal was in good general condition and was found 4 h before death. on necropsy the lungs were congested and oedematous, with haemorrhagic areas in the cranial and middle lobes. the microscopic lesions were those ...200010829577
host specificity of abomasal nematodes in free ranging alpine ruminants.abomasums from 641 alpine wild ruminants representing five different species (cervus elaphus, capreolus capreolus, rupicapra rupicapra, capra ibex, ovis musimon) and from 19 domestic sheep (ovis aries) from alpine areas were examined in order to investigate the host-specificity of abomasal helminths. nine out of 20 helminth species were found in at least five different host species. a discriminant analysis was able to significantly discriminate the hosts on the basis of their helminth community ...200010842002
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