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a preliminary parasitological survey of hepatozoon spp. infection in dogs in mashhad, iran.we attempted to determine the prevalence of hepatozoon spp. infection in mashhad, northeast of iran, via blood smear parasitology.201223323098
hitch-hiker taken for a ride: an unusual cause of myocarditis, septic shock and adult respiratory distress syndrome.rocky mountain spotted fever (rmsf) is a serious tick-borne illness caused by rickettsia rickettsii that is endemic in southeastern usa. although rmsf has been described as causing the classic clinical triad of fever, headache and a characteristic rash, serious and potentially life-threatening manifestations can occur. cardiopulmonary involvement, although infrequent, may occur with severe cases of rmsf. rickettsial myocarditis is an uncommon occurrence. we present a case of a previously healthy ...201323314875
a golden jackal (canis aureus) from austria bearing hepatozoon canis--import due to immigration into a non-endemic area?the protozoan hepatozoon canis, which is transmitted via ingestion of infected ticks by canine hosts, is not endemic to mid-latitude regions in europe. its distribution is supposed to be linked to the occurrence of its primary tick vector rhipicephalus sanguineus. a young male golden jackal (canis aureus) found as road kill close to vienna, austria, was infected by this pathogen. cloning and sequencing of the pcr product revealed 6 different haplotypes of h. canis. based on the sequences, no cle ...201323306030
molecular detection of ehrlichia canis in dogs in malaysia.an epidemiological study of ehrlichia canis infection in dogs in peninsular malaysia was carried out using molecular detection techniques. a total of 500 canine blood samples were collected from veterinary clinics and dog shelters. molecular screening by polymerase chain reaction (pcr) was performed using genus-specific primers followed by pcr using e. canis species-specific primers. ten out of 500 dogs were positive for e. canis. a phylogenetic analysis of the e. canis malaysia strain showed th ...201323301114
prevention of transmission of ehrlichia canis by rhipicephalus sanguineus ticks to dogs treated with a combination of fipronil, amitraz and (s)-methoprene (certifect®).the ability of certifect(®) (a combination of fipronil, amitraz and (s)-methoprene) to prevent transmission of ehrlichia canis was studied in two groups of eight dogs. one group was treated with certifect while the other group remained untreated. all dogs were exposed to e. canis-infected rhipicephalus sanguineus ticks on days 7, 14, 21 and again on day 28 post-treatment by releasing ticks into the kennels of the dogs to simulate the natural way of infestation. animals were examined in situ for ...201323298566
chemical identification of tagetes minuta linnaeus (asteraceae) essential oil and its acaricidal effect on ticks.the control of tick species that affect animal production is vital for the economic welfare of the cattle industry. this study focused on testing the acaricidal activity of the essential oil from the leaves and stems of tagetes minuta against several brazilian tick species, including rhipicephalus (boophilus) microplus, rhipicephalus sanguineus, amblyomma cajennense and argas miniatus. the chemical composition of the essential oil was determined by chromatography and spectroscopy analyses, which ...201323295821
a survey on ectoparasite infestations in companion dogs of ahvaz district, south-west of iran.the objective was to determine the prevalence of ectoparasite infestations in referred companion dogs to veterinary hospital of shahid chamran university of ahvaz, from 2009 to 2010.201223293781
confirmation of the efficacy of a novel fipronil spot-on for the treatment and control of fleas, ticks and chewing lice on dogs.a novel spot-on formulation containing fipronil (eliminall(®)/exproline vet™) spot-on solution for dogs, pfizer animal health, registered and manufactured by krka, d.d, novo mesto) was evaluated in three laboratory studies to confirm efficacy against fleas, ticks and chewing lice on dogs for at least one month. control of two laboratory strains of cat flea (ctenocephalides felis), two tick species (rhipicephalus sanguineus and dermacentor reticulatus) and the chewing louse (trichodectes canis) w ...201223290278
zoonotic pathogens associated with hyalomma aegyptium in endangered tortoises: evidence for host-switching behaviour in ticks?hyalomma aegyptium is a hard-tick with a typical three-host life cycle. the main hosts are palearctic tortoises of genus testudo. however, other hosts can be used by immature ticks for feeding in natural conditions. given this complex ecology and multiple host use, the circulation of pathogens by h. aegyptium between various hosts can be important from epidemiological point of view. the aim of this study was to evaluate the role of h. aegyptium as natural carrier of four important zoonotic patho ...201223273169
on-host control of the brown dog tick rhipicephalus sanguineus latreille (acari: ixodidae) by metarhizium brunneum (hypocreales: clavicipitaceae).ticks are obligatory blood-sucking arthropods. their life cycle includes a relatively short period of feeding on a vertebrate host and a long off-host period spent in the upper layer of the soil. entomopathogenic fungi are known to be highly effective tick pathogens and the on-host application of these fungi may be a promising economic approach for tick control. in this study, we evaluated the tick control provided by spraying metarhizium brunneum onto the tick's vertebrate host, specifically ge ...201323267821
effect of egg clustering on the fitness of rhipicephalus sanguineus larvae.several biological aspects of rhipicephalus sanguineus have been studied, but scant information is available on the effect of egg clustering on incubation period and larval survival. herein, r. sanguineus eggs were separated in groups composed by 1 (gi), 10 (gii), 50 (giii), 100 (giv), 500 (gv) and 1,000 (gvi) eggs (10 replicates), and incubation and larval survival period were recorded. no correlation was observed between the amount of egg batches and hatching percentage. larvae from gi hatched ...201323254589
acaricidal activity of ethanolic extract from aerial parts of tagetes patula l. (asteraceae) against larvae and engorged adult females of rhipicephalus sanguineus (latreille, 1806).the tick rhipicephalus sanguineus is the species with the largest worldwide distribution and is proven to be involved in the transmission of pathogens such as babesia canis, ehrlichia canis, coxiella burnetii, rickettsia ricketsii, rickettsia conorii, among others. studies have demonstrated acquisition of resistance to some of the active principles used in commercial formulations of acaricides. tagetes patula (asteraceae) is a plant with highlighted economic and commercial importance due to the ...201223244493
[detection of rickettsia in ectoparasites of wild and domestic mammals from the cerro chucanti private reserve and from neighboring towns, panamá, 2007-2010].ectoparasites are the main vectors of rickettsiosis. in panama, however, limited data are available concerning the arthropod species that serve as vectors or reservoirs.201223242292
new species of ehrlichia isolated from rhipicephalus (boophilus) microplus shows an ortholog of the e. canis major immunogenic glycoprotein gp36 with a new sequence of tandem repeats.ehrlichia species are the etiological agents of emerging and life-threatening tick-borne human zoonoses that inflict serious and fatal infections in companion animals and livestock. the aim of this paper was to phylogeneticaly characterise a new species of ehrlichia isolated from rhipicephalus (boophilus) microplus from minas gerais, brazil.201223231731
ixodid fauna and zoonotic agents in ticks from dogs: first report of rickettsia rickettsii in rhipicephalus sanguineus in the state of mato grosso do sul, mid-western brazil.ticks from 148 dogs from the urban area of the municipality of campo grande, state of mato grosso do sul, brazil, were collected, classified and analyzed using polymerase chain reaction (pcr) for the identification of rickettsia spp., trypanosoma cruzi and leishmania spp. a total of 2015 ticks were collected. the species rhipicephalus sanguineus (98.9 %) and amblyomma cajennense (1.1 %) were identified. molecular analysis revealed that no tick samples were infected by t. cruzi. regarding leishma ...201323229491
inoculation of salivary gland extracts obtained from female of rhipicephalus sanguineus (latreille, 1806) (acari, ixodidae) with 2, 4, and 6 days of feeding in rabbit: i--histopathology of the feeding lesion.this study analyzed the histopathology of rabbit skin, previously immunized with sge2, sge4, and sge6 gland extracts prepared from salivary glands of rhipicephalus sanguineus female with 2, 4, and 6 days of feeding, at the region of the r. sanguineus female feeding lesion 2, 4, and 6 days after tick attachment. in this work, infestation-naïve new zealand white rabbits were inoculated either with the extracts (test group (tg)) or with phosphate buffer and complete freund's adjuvant mixture (contr ...201323229316
matrix-assisted laser desorption ionization-time of flight mass spectrometry for rapid identification of tick vectors.a method for rapid species identification of ticks may help clinicians predict the disease outcomes of patients with tick bites and may inform the decision as to whether to administer postexposure prophylactic antibiotic treatment. we aimed to establish a matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) spectrum database based on the analysis of the legs of six tick vectors: amblyomma variegatum, rhipicephalus sanguineus, hyalomma marginatum rufipes, ix ...201323224087
transcriptome walking: a laboratory-oriented gui-based approach to mrna identification from deep-sequenced data.deep sequencing technology provides efficient and economical production of large numbers of randomly positioned, relatively short, estimates of base identities in dna molecules. application of this technology to mrna samples allows rapid examination of the molecular genetic environment in individual cells or tissues, the transcriptome. however, assembly of such short sequences into complete mrna creates a challenge that limits the usefulness of the technology, particularly when no, or limited, g ...201223217191
detection of rickettsia massiliae in rhipicephalus sanguineus from the eastern united states.we report the first evidence of rickettsia massiliae in the brown dog tick, rhipicephalus sanguineus, from the east coast of the united states. as part of routine pathogen surveillance, dna samples from ixodid ticks were tested for spotted fever group rickettsiae by nested pcr. a r. massiliae-positive tick was collected off a beagle mix recently rescued from north carolina. infection was confirmed by partial sequence analysis of the htra, glta, ompb, ompa, and sca4 genes, which had 100% identity ...201223199270
forest refugia in western and central africa as 'museums' of mesozoic biodiversity.the refugial speciation model, or 'species pump', is widely accepted in the context of tropical biogeography and has been advocated as an explanation for present species distributions in tropical western and central africa. in order to test this hypothesis, a phylogeny of the cryptic arachnid order ricinulei, based on four nuclear and mitochondrial dna markers, was inferred. this ancient clade of litter-dwelling arthropods, endemic to the primary forests of western and central africa and the neo ...023193047
serological survey on ehrlichia sp. among dogs in the central region of rio grande do sul.a serological survey on ehrlichia canis was conducted among dogs in the central area of the state of rio grande do sul, where the tick rhipicephalus sanguineus is a common parasite of dogs. out of a total of 316 dogs attended at the veterinary teaching hospital in the municipality of santa maria, only 14 (4.43%) reacted positively to e. canis antigens in the indirect immunofluorescence assay, with the following endpoint titers: 80 (three dogs), 160 (five), 320 (four), 640 (one) and 1280 (one). l ...201323184320
detection of leishmania infantum, babesia canis, and rickettsiae in ticks removed from dogs living in italy.the aims of this study were to determine natural infections by anaplasma phagocytophilum/anaplasma platys, bartonella henselae, ehrlichia canis, leishmania infantum, rickettsia spp., babesia spp., and hepatozoon spp. by molecular methods in ticks (n=91) removed from dogs with clinical signs and laboratory abnormalities compatible with tick-borne diseases (n=22) living in italy and to assess the distribution and species of ticks encountered. ticks from dogs living in southern italy were all ident ...201223182545
borrelia, rickettsia, and ehrlichia species in bat ticks, france, 2010.argas vespertilionis, an argasid tick associated with bats and bat habitats in europe, africa, and asia has been reported to bite humans; however, studies investigating the presence of vector-borne pathogens in these ticks are lacking. using molecular tools, we tested 5 a. vespertilionis ticks collected in 2010 from the floor of a bat-infested attic in southwestern france that had been converted into bedrooms. rickettsia sp. avbat, a new genotype of spotted fever group rickettsiae, was detected ...201223171714
tick parasites of rodents in romania: host preferences, community structure and geographical distribution.ticks are among the most important vectors of zoonotic diseases in temperate regions of europe, with widespread distribution and high densities, posing an important medical risk. most ticks feed on a variety of progressively larger hosts, with a large number of small mammal species typically harbouring primarily the immature stages. however, there are certain ixodidae that characteristically attack micromammals also during their adult stage. rodents are widespread hosts of ticks, important vecto ...201223171665
first molecular detection of ehrlichia canis and anaplasma platys in ticks from dogs in cebu, philippines.ehrlichia canis infection of dogs in the philippines has been detected by serological and peripheral blood smear examination methods, but not by molecular means. anaplasma platys infection in dogs has not yet been officially reported, although it is suspected to occur in the country. thus, sensitive and specific molecular techniques were used in this study to demonstrate the presence of both e. canis and a. platys in the philippines. a total of 164 rhipicephalus sanguineus ticks was collected fr ...201223168049
anopheles salivary gland proteomes from major malaria vectors.antibody responses against anopheles salivary proteins can indicate individual exposure to bites of malaria vectors. the extent to which these salivary proteins are species-specific is not entirely resolved. thus, a better knowledge of the diversity among salivary protein repertoires from various malaria vector species is necessary to select relevant genus-, subgenus- and/or species-specific salivary antigens. such antigens could be used for quantitative (mosquito density) and qualitative (mosqu ...201223148599
ehrlichia canis in rhipicephalus sanguineus ticks in the ivory coast.canine monocytic ehrlichiosis caused by ehrlichia canis is distributed globally, but its prevalence in africa is poorly known. in the study reported herein, 27% of rhipicephalus sanguineus ticks collected from watchdogs in abidjan, ivory coast, were positive for e. canis using quantitative real-time pcr. a new molecular strategy is proposed that can be used not only for epidemiological study, but also for the diagnosis of canine monocytic ehrlichiosis. our findings show for the first time the pr ...201223141106
urban family cluster of spotted fever rickettsiosis linked to rhipicephalus sanguineus infected with rickettsia conorii subsp. caspia and rickettsia massiliae.here, we report an epidemiological and entomological investigation of a cluster of cases of spotted fever group (sfg) rickettsiosis occurring in southern france. a family of 3 (husband, wife, and their son) presented with symptoms compatible with sfg rickettsiosis. for 2 patients, serum samples presented increased levels of igm and igg for sfg rickettsia. the patients' home was investigated, and rhipicephalus sanguineus ticks were collected from the floor from behind the furniture. of 22 ticks c ...201223140893
spotted fever group rickettsiae in ticks and fleas from the democratic republic of the congo.little is known about the prevalence of spotted fever group (sfg) rickettsiae in ticks and fleas in the democratic republic of the congo. in 2008, 12 amblyomma compressum ticks were collected from 3 pangolins (manis gigantea). two haemaphysalis punctaleachi ticks were collected from 2 african civets (civettictis civetta congica), and one was collected from an antelope (onotragus leche). a total of 111 rhipicephalus sanguineus ticks, 23 ctenocephalides canis fleas, 39 c. felis fleas, and 5 tricho ...201223137572
rhipicephalus sanguineus (acari: ixodidae) female ticks exposed to castor oil (ricinus communis): an ultrastructural overview.tick control has been accomplished through the use of synthetic acaricides, which has created resistant individuals, as well as contaminating the environment and nontarget organisms. substances of plant origin, such as oils and extracts of eucalyptus and neem leaves, have been researched as an alternative to replace the synthetic acaricides. ricinoleic acid esters from castor oil have recently been shown as a promising alternative in eliminating bacterial contamination during ethanol fermentatio ...201323086445
experimental evaluation of birds as disseminators of the cosmopolitan tick rhipicephalus sanguineus (acari: ixodidae).rhipicephalus sanguineus is believed to be the most widespread tick species of the world and its dissemination seems to rely on the diffusion of its main host, the dog. empirical observations indicate that several bird species in urban areas regularly steal dog food. such circumstances create a chance for r. sanguineus ticks to climb on birds and carry ticks to another site. in this work we evaluated experimentally the likelihood of birds (chicks) to either feed and/or carry r. sanguineus ticks ...201223078993
tick-box for 3'-end formation of mitochondrial transcripts in ixodida, basal chelicerates and drosophila.according to the trna punctuation model, the mitochondrial genome (mtdna) of mammals and arthropods is transcribed as large polycistronic precursors that are maturated by endonucleolytic cleavage at trna borders and rna polyadenylation. starting from the newly sequenced mtdna of ixodes ricinus and using a combination of mitogenomics and transcriptional analyses, we found that in all currently-sequenced tick lineages (prostriata, metastriata and argasidae) the 3'-end of the polyadenylated nad1 an ...201223077630
abiotic and biotic factors associated with tick population dynamics on a mammalian host: ixodes hexagonus infesting otters, lutra lutra.the eurasian otter, lutra lutra, hosts several parasites with zoonotic potential. as this semiaquatic mammal has large ranges across terrestrial, freshwater and marine habitats, it has the capacity for wide dispersion of pathogens. despite this, parasites of otters have received relatively little attention. here, we examine their ectoparasite load and assess whether this is influenced by abiotic or biotic variables. climatic phenomena such as the north atlantic oscillation (nao) affect weather c ...201223071736
parasites of domestic and wild canids in the region of serra do cipó national park, brazil.over recent decades, diseases have been shown to be important causes of extinctions among wild species. greater emphasis has been given to diseases transmitted by domestic animals, which have been increasing in numbers in natural areas, along with human populations. this study had the aim of investigating the presence of intestinal helminths in wild canids (maned wolf, chrysocyon brachyurus, and crab-eating fox, cerdocyon thous) in the serra do cipó national park (43-44º w and 19-20º s) and endo ...201223070438
bioinformatics and expression analyses of the ixodes scapularis tick cystatin family.the cystatins are inhibitors of papain- and legumain-like cysteine proteinases, classified in merops subfamilies i25a-i25c. this study shows that 84 % (42/50) of tick cystatins are putatively extracellular in subfamily i25b and the rest are putatively intracellular in subfamily i25a. on the neighbor joining phylogeny guide tree, subfamily i25a members cluster together, while subfamily i25b cystatins segregate among prostriata or metastriata ticks. two ixodes scapularis cystatins, aay66864 and is ...201223053911
morphology of the midgut of rhipicephalus sanguineus (latreille, 1806) (acari: ixodidae) adult ticks in different feeding stages.the intestinal epithelial cells of ticks are fundamental for their full feeding and reproductive success, besides being considered important sites for the development of pathogens. rhipicephalus sanguineus ticks are known for their great medical and veterinary importance, and for this reason, the knowledge of their intestinal morphology may provide relevant subsidies for the control of these animals, either by direct acaricidal action over these cells or by the production of vaccines. therefore, ...201323052783
effect of ricinoleic acid esters from castor oil (ricinus communis) on the oocyte yolk components of the tick rhipicephalus sanguineus (latreille, 1806) (acari: ixodidae).rhipicephalus sanguineus are bloodsucking ectoparasites, whose main host is the domestic dog, thus being present in urban areas and closely located to people. eventually, this tick species parasitize humans and can become a potential vector of infectious diseases. methods to control this type of pest have been the focus of many research groups worldwide. the use of natural products is increasingly considered nowadays, due to the low toxicity levels to the host and low waste generation to the env ...201323040769
identification of salivary antigenic markers discriminating host exposition between two european ticks: rhipicephalus sanguineus and dermacentor reticulatus.to succeed blood meal, ticks inject salivary proteins to mammalian hosts, eliciting an antibody response against these foreign antigens. although this immune response has been proposed as a surrogate marker of exposure to tick bites, identification of the corresponding antigens remains elusive. for this aim, a comparison by immunoblots of the kinetic igg responses to protein salivary gland extracts from two european tick species, rhipicephalus sanguineus or dermacentor reticulatus, in rabbits wa ...201323040662
candidatus bartonella merieuxii, a potential new zoonotic bartonella species in canids from iraq.bartonellae are emerging vector-borne pathogens infecting erythrocytes and endothelial cells of various domestic and wild mammals. blood samples were collected from domestic and wild canids in iraq under the united states army zoonotic disease surveillance program. serology was performed using an indirect immunofluorescent antibody test for b. henselae, b. clarridgeiae, b. vinsonii subsp. berkhoffii and b. bovis. overall seroprevalence was 47.4% in dogs (n = 97), 40.4% in jackals (n = 57) and 12 ...201223029597
a survey on endoparasites and ectoparasites of stray cats from mashhad (iran) and association with risk factors.as there appeared to be no data available on parasite infection of stray cats in the region and considering the potential threat of stray cats for animal and public health, the present study was carried out using biological samples and necropsy finding collected from cats captured in mashhad city in the northeast of iran. from a total 52 stray cats examined, 18 (34.6%) were male and 34 (65.4%) were female. ten species of endoparasites including helminthes and protozoa and two species of ectopara ...201123024505
serodetection of ehrlichia canis infection in dogs from ludhiana district of punjab, india.dot-elisa (enzyme-linked immunosorbent assay) immunocomb(®) assay was conducted to detect the presence of antibodies against ehrlichia canis in blood samples of 60 privately owned dogs suspected to be infected with e. canis from the small animal clinics, college of veterinary science, guru angad dev veterinary and animal sciences university, ludhiana, punjab (india). antibodies reactive to e. canis were detected in plasma in 48 samples out of 60 samples by immunocomb(®) dot-elisa. out of these 3 ...201123024503
fluazuron-induced morphophysiological changes in the cuticle formation and midgut of rhipicephalus sanguineus latreille, 1806 (acari: ixodidae) nymphs.the present study demonstrated the effects of the arthropod growth regulator, fluazuron (acatak®), in the formation of the integument and digestive processes of rhipicephalus sanguineus nymphs fed on rabbits treated with different doses of this chemical acaricide. for this, three different doses of fluazuron (20, 40, or 80 mg/kg) were applied "pour on" to the hosts (groups ii, iii, and iv), as well as distilled water to the control group. on the first day after treatment (24 h), the hosts were a ...201322992894
unusual bacterial infections and the pleura.rickettsiosis, q fever, tularemia, and anthrax are all bacterial diseases that can affect the pleura. rocky mountain spotted fever (rmsf) and mediterranean spotted fever (msf) are caused by rickettsia rickettsii and rickettsia conorii, respectively. pleural fluid from a patient with msf had a neutrophil-predominant exudate. coxiellaburnetii is the causative agent of q fever. of the two cases described in the literature, one was an exudate with a marked eosinophilia while the other case was a tra ...201222977649
an assessment of whole blood and fractions by nested pcr as a dna source for diagnosing canine ehrlichiosis and anaplasmosis.ehrlichiosis and anaplasmosis are tick-borne diseases. ehrlichia canis and anaplasma platys infect mainly white cells and platelets, respectively. the main dna source for pcr is peripheral blood, but the potential of blood cell fractions has not been extensively investigated. this study aims at assessment of whole blood (wb) and blood fractions potential in nested pcr (npcr) to diagnose canine ehrlichiosis and anaplasmosis. the 16s rrna gene was amplified in 71.4, 17.8, 31.57, and 30% of the wb, ...201222973174
action of andiroba oil (carapa guianensis) on rhipicephalus sanguineus (latreille, 1806) (acari: ixodidae) semi-engorged females: morphophysiological evaluation of reproductive system.because of the increasing medical-veterinary importance of ticks, the development of alternative control methods, less aggressive to the host and the environment has become the target of several researches. in this sense, the present study analyzed the action of different concentrations (5, 10, and 20%) of andiroba seed oil (carapa guianensis) on the reproductive system of rhipicephalus sanguineus females, through histochemical techniques and the quantification of the reproductive efficiency ind ...201222972770
migratory birds, ticks, and bartonella.bartonella spp. infections are considered to be vector-borne zoonoses; ticks are suspected vectors of bartonellae. migratory birds can disperse ticks infected with zoonotic pathogens such as rickettsia and tick-borne encephalitis virus and possibly also bartonella. thus, in the present study 386 tick specimens collected in spring 2009 from migratory birds on the mediterranean islands capri and antikythera were screened for bartonella spp. rna. one or more ticks were found on 2.7% of the birds. m ...201122957116
[survey on ticks and detection of new bunyavirus in some vect in the endemic areas of fever, thrombocytopenia and leukopenia syndrome (ftls) in henan province].to investigate the distribution, species, seasonal fluctuation of ticks and detect new bunyavirus in some hematophagus in the endemic areas of fever thrombocytopenia and leukopenia syndrome (ftls) in henan province.201222943894
genome sequence of rickettsia conorii subsp. israelensis, the agent of israeli spotted fever.rickettsia conorii subsp. israelensis is the agent of israeli spotted fever. the present study reports the draft genome of rickettsia conorii subsp. israelensis strain istt cdc1, isolated from a rhipicephalus sanguineus tick collected in israel.022933760
off-host aggregation in the non-fed, female brown dog tick, rhipicephalus sanguineus (latreille), is induced by tick excreta and enhanced by low relative humidity.we report that rhipicephalus sanguineus (ixodida: ixodidae) faeces and its main component, guanine, act as assembly pheromones in short-range petri plate bioassays. arrestment activity in response to guanine was lower than that in response to natural excreta, indicating the presence of other active ingredients in natural excreta. the selective removal of appendages was used to establish the important roles played by the palps and the front pair of legs in the detection of the pheromone. reaction ...201322928633
rickettsia species in ticks removed from humans in istanbul, turkey.a total of 167 ticks collected from humans in istanbul (turkey) in 2006 were screened for rickettsia species, and nested pcrs targeting glta and ompa rickettsial fragment genes were carried out. rickettsia monacensis (51), r. aeschlimannii (8), r. conorii subsp. conorii (3), r. helvetica (2), r. raoultii (1), r. africae (1), r. felis (1), and other rickettsia spp. (2), were detected. to our knowledge, these rickettsia species (except r. conorii) had never been reported in ticks removed from huma ...201222925016
some epidemiological features of the mediterranean spotted fever re-emerging in bulgaria.the mediterranean spotted fever (msf), caused by rickettsia conorii conorii strain malish, is transmitted by the brown dog tick rhipicephalus sanguineus. in bulgaria, cases of msf occurred in two epidemic waves, the first in 1948-1970, (after there were no report of new cases more than for 20 years) and the second started in 1993 and is still going on. the aim of the study was to investigate the epidemiological characteristics of the re-emerging msf in plovdiv city and its suburbs, which is the ...201222908829
identification of endosymbionts in ticks by broad-range polymerase chain reaction and electrospray ionization mass spectrometry.many organisms, such as insects, filarial nematodes, and ticks, contain heritable bacterial endosymbionts that are often closely related to transmissible tickborne pathogens. these intracellular bacteria are sometimes unique to the host species, presumably due to isolation and genetic drift. we used a polymerase chain reaction/electrospray ionization-mass spectrometry assay designed to detect a wide range of vectorborne microorganisms to characterize endosymbiont genetic signatures from amblyomm ...201222897044
genome sequence of rickettsia conorii subsp. caspia, the agent of astrakhan fever.rickettsia conorii subsp. caspia is the agent of astrakhan fever, a spotted fever group rickettsiosis endemic to astrakhan, russia. the present study reports the draft genome of rickettsia conorii subsp. caspia strain a-167.022887666
a fatal urban case of rocky mountain spotted fever presenting an eschar in san jose, costa rica.this study reports the first urban human case of rocky mountain spotted fever caused by rickettsia rickettsii, in costa rica. an 8-year-old female who died at the national children's hospital 4 days after her admission, and an important and significant observation was the presence of an "eschar" (tache noire), which is typical in some rickettsial infections but not frequent in rocky mountain spotted fever cases.201222855769
association of environmental traits with the geographic ranges of ticks (acari: ixodidae) of medical and veterinary importance in the western palearctic. a digital data set.we compiled information on the distribution of ticks in the western palearctic (11°w, 45°e; 29°n, 71°n), published during 1970-2010. the literature search was filtered by the tick's species name and an unambiguous reference to the point of capture. records from some curated collections were included. we focused on tick species of importance to human and animal health, in particular: ixodes ricinus, dermacentor marginatus, d. reticulatus, haemaphysalis punctata, h. sulcata, hyalomma marginatum, h ...201222843316
ectoparasites of dogs in home environments on the caribbean slope of costa rica.reports on ectoparasites on dogs in central america are scarce. the aim of this study was to identify flea, louse and tick species infesting dogs in home environments on the caribbean slope of costa rica, and determine their frequency and coexistence. ectoparasites were collected from dogs in 83 rural homes at five study sites. specimens were identified and separated according to species. fleas were the most common ectoparasite (g = 22,217, df = 8, p = 0.004). ctenocephalides felis and pulex sim ...201322832763
high prevalence of intestinal infections and ectoparasites in dogs, minas gerais state (southeast brazil).in the present study, 155 dogs euthanized by the zoonotic disease unit of uberlândia in minas gerais state (southeast brazil) were autopsied. ectoparasites were collected, and the intestinal content of dogs was systematically examined for the presence of helminthic parasites. in total, we isolated 5,155 metazoan parasites of eight species (three intestinal helminth species, five ectoparasite species). the cestode dipylidium caninum was present in 57 dogs (36.8 %), the nematodes ancylostoma canin ...201222825284
mitochondrial dna analysis of rhipicephalus sanguineus sensu lato (acari: ixodidae) in the southern cone of south america.a genetic analysis of partial sequences of the mitochondrial 16s and 12s rdna genes of rhipicephalus sanguineus sensu lato collected in the southern cone of south america was carried out. also, sequences of ticks belonging to this taxon from europe, africa and other south american localities were included. tcs networks constructed with 16s rdna sequences showed two clusters of haplotypes, namely, southern lineage (st) and northern lineage (nt). haplotypes representing the specimens coming from l ...201222818199
pcr-detection of coxiella burnetii in ticks collected from sheep and goats in southeast iran.there is a little data on coxiella burnetii (q fever agent) in iran. ticks may play a significant role in the transmission of c. burnetii among animals. the aim of this study was to use polymerase chain reaction for the detection of c. burnetii in ticks collected in southeast iran.201122808404
hard ticks on domestic ruminants and their seasonal population dynamics in yazd province, iran.ticks are the main vectors for transmission of different pathogens to human and animals. this survey was performed to find out distribution of ticks, which infested the domestic ruminants in yazd province, central iran during year 2008-2009.201022808391
[investigation of the cases presenting to dokuz eylül university hospital with tick bites].determination of the properties of ticks, which are the vectors of many infectious diseases and the patients with tick bites are important for the prevention of these diseases. for tist reason, the purpose of this study is to determine the important properties of the cases presenting with tick bites to dokuz eylul university hospital and of the removed ticks from the cases.201222801910
cytotoxic effects of andiroba oil (carapa guianensis) in reproductive system of rhipicephalus sanguineus (latreille, 1806) (acari: ixodidae) semi-engorged females.the present study performed an analysis about the effects of andiroba seed oil (carapa guianensis) in the ovary of rhipicephalus sanguineus semi-engorged females; once, there are few studies about the action of natural products on the reproductive system, a vital organ for the biological success of this animal group. the results showed that andiroba oil is a potent natural agent which causes significant structural changes in the oocytes, such as the emergence of large vacuolated cytoplasmic regi ...201222797575
survey of borreliae in ticks, canines, and white-tailed deer from arkansas, u.s.a.in the eastern and upper midwestern regions of north america, ixodes scapularis (l.) is the most abundant tick species encountered by humans and the primary vector of b. burgdorferi, whereas in the southeastern region amblyomma americanum (say) is the most abundant tick species encountered by humans but cannot transmit b. burgdorferi. surveys of borreliae in ticks have been conducted in the southeastern united states and often these surveys identify b. lonestari as the primary borrelia species, ...201222781030
anaplasma phagocytophilum infection in domestic animals in ten provinces/cities of china.a nationwide epidemiologic investigation of domestic animal infections has been conducted in nine provinces and one city during 2007-2010. serum samples from a total of 707 goats, 433 cattle, and 219 dogs were collected for detecting anaplasma phagocytophilum igg antibody by immunofluorescence assays and the average seroprevalences were 10.05% for dogs, 3.82% for goats, and 0.69% for cattle, respectively. a total of 472 goats, 201 cattle, 102 dog blood clots, and 1,580 ticks were collected for p ...022764312
action of permethrin on rhipicephalus sanguineus (latreille, 1806) (acari: ixodidae) semi-engorged females: morpho-physiological evaluation of salivary glands.currently the most effective method of tick control is the use of acaricides, among which stands out permethrin (active ingredient of acaricide advantage(®) max3, bayer), a neurotoxic pyrethroid. however, assessments of their effects on other tick systems such as glandular are still scarce. thus, this study provides information, through histochemical techniques, about the toxic effect of this pyrethroid on the morphophysiology of salivary glands of semi-engorged rhipicephalus sanguineus females ...201222749738
why are there so few rickettsia conorii conorii-infected rhipicephalus sanguineus ticks in the wild?rickettsia conorii conorii is the etiological agent of mediterranean spotted fever, which is transmitted by the brown dog tick, rhipicephalus sanguineus. the relationship between the rickettsia and its tick vector are still poorly understood one century after the first description of this disease.201222724035
epidemiology of brazilian spotted fever in the atlantic forest, state of são paulo, brazil.the tick-borne bacterium rickettsia rickettsii is the aetiological agent of brazilian spotted fever (bsf). the present study evaluated tick infestations on wild and domestic animals, and the rickettsial infection in these animals and their ticks in 7 forest areas adjacent to human communities in the são paulo metropolitan area (spma). the results were compared to ecological traits of each sampled area. two main tick species, amblyomma aureolatum and rhipicephalus sanguineus, were collected from ...201222716923
cutaneous distribution and localization of cercopithifilaria sp. microfilariae in dogs.the aim of this study was to investigate the anatomical distribution of microfilariae of a recently described cercopithifilaria sp. and the relationship with the preferred attachment sites of its vector, the brown dog tick rhipicephalus sanguineus. skin samples from 20 dogs were collected from eight anatomical sites and soaked twice in saline solution at 37°c. all samples were also molecularly processed for the specific amplification of partial cytochrome c oxidase 1 gene. microfilariae were une ...201222698796
tick paralysis: first zoonosis record in egypt.tick paralysis caused by the secretion of toxin with saliva while taking a blood meal is an important veterinary disease, but is rare in humans. although it has certain geographical proclivities, it exists worldwide. tick paralysis was demonstrated for the first time in egypt among four children living in rural area at giza governorate. the clinical pictures were confused with rabies; myasthensia gravis; botulism; diphtheritic polyneuropathy encountered in rural areas. the recovery of tick infes ...201222662597
molecular method for the characterization of coxiella burnetii from clinical and environmental samples: variability of genotypes in spain.coxiella burnetii is a highly clonal microorganism which is difficult to culture, requiring bsl3 conditions for its propagation. this leads to a scarce availability of isolates worldwide. on the other hand, published methods of characterization have delineated up to 8 different genomic groups and 36 genotypes. however, all these methodologies, with the exception of one that exhibited limited discriminatory power (3 genotypes), rely on performing between 10 and 20 pcr amplifications or sequencing ...201222656068
efficacy of an imidacloprid/flumethrin collar against fleas, ticks, mites and lice on dogs.the studies reported here were conducted to ascertain the efficacy of imidacloprid/flumethrin incorporated in a slow-release matrix collar, against infestations of dogs by fleas, ticks, mites and lice. efficacy was evaluated against the flea ctenocephalides felis felis, the ticks rhipicephalus sanguineus, ixodes ricinus, ixodes scapularis, dermacentor reticulatus and dermacentor variabilis, the mite sarcoptes scabiei and the biting louse trichodectes canis.201222647530
rickettsia parkeri: a rickettsial pathogen transmitted by ticks in endemic areas for spotted fever rickettsiosis in southern uruguay.at first rickettsia conorii was implicated as the causative agent of spotted fever in uruguay diagnosed by serological assays. later rickettsia parkeri was detected in human-biting amblyomma triste ticks using molecular tests. the natural vector of r. conorii, rhipicephalus sanguineus, has not been studied for the presence of rickettsial organisms in uruguay. to address this question, 180 r. sanguineus from dogs and 245 a. triste from vegetation (flagging) collected in three endemic localities w ...201222634883
genome sequence of rickettsia conorii subsp. indica, the agent of indian tick typhus.rickettsia conorii subsp. indica is the agent of indian tick typhus. the present study reports the draft genome of rickettsia conorii subsp. indica strain ittr (atcc vr-597).022628514
copulation is necessary for the completion of a gonotrophic cycle in the tick rhipicephalus sanguineus (latreille, 1806) (acari: ixodidae).ovarian development and egg maturation are essential stages in animal reproduction. for bisexual ixodid ticks, copulation is an important prerequisite for the completion of the gonotrophic cycle. in this study, we aimed to characterize the morpho-histological changes in the ovary and oocytes of the tick rhipicephalus sanguineus, together with the identification of feeding and reproductive parameters associated with mating. virgin and cross-mated females (with r. turanicus males) weighed 60% less ...201222626791
morphological characterization of the nymphs rhipicephalus sanguineus ticks (latreille, 1806) (acari: ixodidae). description of the testes, integument, malpighian tubules, and midgut on the detachment day.this study presents the morpho-histological and histochemical characterization of the testes, integument, malpighian tubules, and midgut of engorged rhipicephalus sanguineus nymphs on the detachment day, showing the morphological and physiological characteristics to this phase in the life cycle of these individuals. the testis is constituted by germinative cells (only spermatogonia) with large, round-shaped and strongly stained nuclei which are organized into cysts by a thin layer of somatic cel ...201222615106
detection of leishmania infantum dna mainly in rhipicephalus sanguineus male ticks removed from dogs living in endemic areas of canine leishmaniosis.sand flies are the only biologically adapted vectors of leishmania parasites, however, a possible role in the transmission of leishmania has been proposed for other hematophagous ectoparasites such as ticks. in order to evaluate natural infection by leishmania infantum in rhipicephalus sanguineus ticks, taking into account its close association with dogs, 128 adult r. sanguineus ticks removed from 41 dogs living in endemic areas of canine leishmaniosis were studied.201222613502
central nervous system of rhipicephalus sanguineus ticks (acari: ixodidae): an ultrastructural study.this study performed the ultrastructural description of the synganglion of rhipicephalus sanguineus males and females, aiming to contribute to the understanding of the cellular organization of this organ. the results show that the central nervous system of these individuals consists of a mass of fused nerves, named synganglion, from where nerves emerge towards several parts of the body. it is surrounded by the neural lamella, a uniform and acellular layer, constituted by repeated layers of homog ...201222610445
effect of calea serrata less. n-hexane extract on acetylcholinesterase of larvae ticks and brain wistar rats.acetylcholinesterase (ache), an enzyme that hydrolyses acetylcholine (ach) at cholinergic synapses, is a target for pesticides and its inhibition by organophosphates leads to paralysis and death of arthropods. it has been demonstrated that the n-hexane extract of calea serrata had acaricidal activity against larvae of rhipicephalus (boophilus) microplus and rhipicephalus sanguineus. the aim of the present study was to understand the mechanism of the acaricidal action of c. serrata n-hexane extra ...201222608349
haemoparasites of free-roaming dogs associated with several remote aboriginal communities in australia.tick-borne haemoparasites babesia vogeli and anaplasma platys are common among the free-roaming canine populations associated with aboriginal communities in australia, whilst the prevalence of haemoplasmas, which are also suspected to be tick-borne, remained unexplored. the aim of this study was to determine the prevalence of haemoplasma infection in these populations, and to identify any correlation with other haemoparasites. blood was collected from 39 dogs associated with four aboriginal comm ...201222584120
ectoparasites of livestock, dogs, and wild rodents in the chittagong hill tracts in southeastern bangladesh.a large variety of ectoparasites parasitizing on livestock, dogs, and rodents are documented throughout the world, of which several are proven vectors for major (including zoonotic) diseases affecting humans and/or livestock. however, there remains a significant lack of knowledge in regard to the ectoparasite fauna in remote regions of the developing world, such as southeastern bangladesh, and an urgent need to investigate this fauna to improve diagnostic options. in the course of the present st ...201222576854
multiple live subconjunctival dipetalonema: report of a case.parasitic infestations of the eye have been reported since centuries, affecting various parts of the eye. some are subtle, coexisting with vision, while many others damage and destroy, in part or totally, the gift of sight. this report describes a patient with live subconjunctival dipetalonema infestation of the right eye, with 22 parasites removed live in one sitting from one eye.022569390
morphological, biological and molecular characteristics of bisexual and parthenogenetic haemaphysalis longicornis.the reproductive mechanism of haemaphysalis longicornis is quite different from many other animal species. in this article, several characteristics of parthenogenetic and bisexual populations of h. longicornis were analyzed, including some important micro-structures, synchronized life cycle feature and sequences of mitochondrial 16s rrna gene. the results suggested even though many observations of the two populations were similar to each other, some important differences also existed. the genita ...201222560314
apparent tick paralysis by rhipicephalus sanguineus (acari: ixodidae) in dogs.certain tick species including ixodes holocyclus can inoculate neurotoxins that induce a rapid, ascending flaccid paralysis in animals. rhipicephalus sanguineus, the most widespread tick of dogs, is recognized as a vector of several pathogens causing diseases in dogs and humans. a single report suggests its role as cause of paralysis in dogs. this study presents the clinical history of 14 young dogs heavily infested by r. sanguineus (intensity of infestation, 63-328) in an endemic area of southe ...201222546547
synopsis of the hard ticks (acari: ixodidae) of romania with update on host associations and geographical distribution.the current paper is a synoptic review of the distribution and host associations of the 25 species of hard tick fauna (family ixodidae) in romania. in addition to a full literature survey, original data is presented, based on eight years of occasional or targeted sample collection. the literature data on geographical distribution was transposed digitally to the decimal degree coordinate system. for each species, an updated distribution map is given together with all historical data and new host ...201222544174
ticks (ixodida) on humans from central panama, panama (2010-2011).from january 2010 to december 2011, a total of 138 cases of ticks feeding on humans were reported from 11 locations in central panama. five of these locations were situated in forest environments, three in rural landscapes and three in urban areas. the ticks were submitted to the gorgas memorial institute, where nine species were identified among 65 specimens: amblyomma cajennense s.l., a. dissimile, a. naponense, a. oblongoguttatum, a. ovale, a. sabanerae, a. tapirellum, haemaphysalis juxtakoch ...201222544074
efficacy of an imidacloprid/flumethrin collar against fleas and ticks on cats.the objectives of the studies listed here were to ascertain the therapeutic and sustained efficacy of 10% imidacloprid (w/w) and 4.5% flumethrin (w/w) incorporated in a slow-release matrix collar, against laboratory-infestations of fleas and ticks on cats. efficacy was evaluated against the flea ctenocephalides felis felis, and the ticks ixodes ricinus, amblyomma americanum and rhipicephalus turanicus. the number of studies was so large that only a general overview can be presented in this abstr ...201222541037
crossbreeding between different geographical populations of the brown dog tick, rhipicephalus sanguineus (acari: ixodidae).brown dog ticks are distributed world-wide, and their systematics and phylogeny are the subject of an ongoing debate. the present study evaluates the reproductive compatibility between rhipicephalus sanguineus ticks from north america, israel, and africa. female ticks of the parent generation were mated with males from the same and alternate colonies. every pure and hybrid cohort was maintained separately into the f2 generation with f1 females being allowed to mate only with males from the same ...201222527838
ectoparasite infestation patterns of domestic dogs in suburban and rural areas in borneo.domestic dogs, canis lupus, have been one of the longest companions of humans and have introduced their own menagerie of parasites and pathogens into this relationship. here, we investigate the parasitic load of 212 domestic dogs with fleas (siphonaptera) chewing lice (phthiraptera), and ticks (acarina) along a gradient from rural areas with near-natural forest cover to suburban areas in northern borneo (sabah, malaysia). we used a spatially-explicit hierarchical bayesian model that allowed us t ...201222526293
efficacy of slow-release collar formulations of imidacloprid/flumethrin and deltamethrin and of spot-on formulations of fipronil/(s)-- methoprene, dinotefuran/pyriproxyfen/permethrin and (s) -methoprene/amitraz/fipronil against rhipicephalus sanguineus and ctenocephalides felis felis on dogs.two studies evaluating the efficacy of an imidacloprid/flumethrin collar (seresto®, bayer animal health, ivp), a deltamethrin collar (scalibor®, msd, cp1), a fipronil/(s)-methoprene spot-on (frontline combo®, merial, cp2), a dinotefuran/pyriproxyfen/permethrin spot-on (vectra 3d®, ceva, cp3) and an amitraz/fipronil/(s)-methoprene spot-on (certifect®, merial, cp4/cp5) against repeated infestations with rhipicephalus sanguineus and ctenocephalides felis felis on dogs were conducted over periods of ...201222520338
resistant ticks inhibit metarhizium infection prior to haemocoel invasion by reducing fungal viability on the cuticle surface.we studied disease progression of, and host responses to, four species in the metarhizium anisopliae complex expressing green fluorescent protein (gfp). we compared development and determined their relative levels of virulence against two susceptible arthropods, the cattle tick rhipicephalus annulatus and the lepidopteran galleria mellonella, and two resistant ticks, hyalomma excavatum and rhipicephalus sanguineus. metarhizium brunneum ma7 caused the greatest mortality of r. annulatus, metarhizi ...201222507442
the synergistic action of imidacloprid and flumethrin and their release kinetics from collars applied for ectoparasite control in dogs and cats.the control of tick and flea burdens in dogs and cats has become essential to the control of important and emerging vector borne diseases, some of which are zoonoses. flea worry and flea bite hypersensitivity are additionally a significant disease entity in dogs and cats. owner compliance in maintaining the pressure of control measures has been shown to be poor. for these reasons efforts are continuously being made to develop ectoparasiticides and application methods that are safe, effective and ...201222498105
fatal rocky mountain spotted fever in the united states, 1999-2007.death from rocky mountain spotted fever (rmsf) is preventable with prompt, appropriate treatment. data from two independent sources were analyzed to estimate the burden of fatal rmsf and identify risk factors for fatal rmsf in the united states during 1999-2007. despite increased reporting of rmsf cases to the centers for disease control and prevention, no significant changes in the estimated number of annual fatal rmsf cases were found. american indians were at higher risk of fatal rmsf relativ ...201222492159
prevalence of tick infestation (rhipicephalus sanguineus and hyalomma anatolicum anatolicum) in dogs in punjab, pakistan.this study was conducted to identify ticks that infest dogs in the rawalpindi region of punjab, pakistan. a total of 525 dogs were examined between june and september 2010. of these, 60 (11.42%) were infested with ticks. the morphological features of the ticks were identified by using a stereomicroscope to confirm their identity using morphological keys. two species were observed, namely: rhipicephalus sanguineus and hyalomma anatolicum anatolicum. the prevalence rate of these species was 98.33% ...201322485006
ecdysteroid levels changed by permethrin action in female rhipicephalus sanguineus (latreille, 1806) (acari: ixodidae) ticks.as recent studies have shown that ecdysteroids may play a major role in the regulation of vitellogenesis in ixodidae, the present study quantified, by means of a radioimmunoassay, the levels of ecdysteroids present in the hemolymph of semi-engorged females of rhipicephalus sanguineus ticks obtained from control females (exposed to distilled water) and those exposed to increasing concentrations of permethrin. the levels of ecdysteroids decreased significantly as the concentration of permethrin in ...201222483974
rickettsia monacensis as cause of mediterranean spotted fever-like illness, italy. 022469314
composition and seasonal variation of rhipicephalus turanicus and rhipicephalus sanguineus bacterial communities.a 16s rrna gene approach, including 454 pyrosequencing and quantitative pcr (qpcr), was used to describe the bacterial community in rhipicephalus turanicus and to evaluate the dynamics of key bacterial tenants of adult ticks during the active questing season. the bacterial community structure of rh. turanicus was characterized by high dominance of coxiella and rickettsia and extremely low taxonomic diversity. parallel diagnostic pcr further revealed a novel coxiella species which was present and ...201222467507
potential of the insect growth regulator, fluazuron, in the control of rhipicephalus sanguineus nymphs (latreille, 1806) (acari: ixodidae): determination of the ld95 and ld50.conventional pesticides have suffered two main drawbacks: (a) broad spectrum of action and (b) selection of target species resistant to the different active ingredients. thus compounds that are less harmful to the environment and to human health, more specific and that do not induce resistance need to be developed. one alternative are insect growth regulators, such as fluazuron. the present study examined the efficacy of fluazuron (active ingredient of the acaricide acatak®) and the sensitivity ...201222465612
evaluation of the long-term efficacy and safety of an imidacloprid 10%/flumethrin 4.5% polymer matrix collar (seresto®) in dogs and cats naturally infested with fleas and/or ticks in multicentre clinical field studies in europe.the objective of these two gcp multicentre european clinical field studies was to evaluate the long-term efficacy and safety of a new imidacloprid/flumethrin collar (seresto®, bayer animalhealth, investigational veterinary product(ivp)) in dogs and cats naturally infested with fleas and/or ticks in comparison to a dimpylat collar ("ungezieferband fuer hunde/fuer katzen", beaphar, control product (cp)).201222463745
ecological approaches to informing public health policy and risk assessments on emerging vector-borne zoonoses.pathogens associated with vector-borne zoonoses occur in enzootic cycles within nature. they are driven by a combination of vertebrate host and invertebrate vector population dynamics, which in turn respond to changes in environmental stimuli. human involvement in these cycles, and hence the occurrence of human disease, is often to act as incidental host. from a public health perspective our ability to better predict human outbreaks of these diseases and prepare intervention and mitigation strat ...201022460391
occurrence of pathogenic fungi to amblyomma cajennense in a rural area of central brazil and their activities against vectors of rocky mountain spotted fever.two isolates of beauveria bassiana and one of purpureocillium lilacinum (=paecilomyces lilacinus) were found infecting amblyomma cajennense engorged females collected on horses (0.15% infection rate from a total of 1982 specimens) and another two isolates of p. lilacinum and one metarhizium anisopliae detected in soils (2.1% from 144 samples) collected in typical pasture habitats of this tick in central brazil from october 2009 to march 2011. fungi were isolated from soils with rhipicephalus san ...201222459109
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