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rhipicephalus sanguineus importation into the uk: surveillance, risk, public health awareness and one health response.as part of public health england's assessment of vectorborne disease risk to public health in the uk, tick specimens are regularly submitted by veterinarians for identification via the tick surveillance scheme. recently, a number of these specimens have been identified as the brown dog tick, rhipicephalus sanguineus this species is non-endemic to the uk and presents a risk to both human and animal health due to its role in the transmission of various tickborne pathogens. although current climati ...201727999153
rhipicephalus turanicus, a new vector of hepatozoon canis.the distribution of hepatozoon canis mainly encompasses areas where its main tick vector, rhipicephalus sanguineus sensu lato, is present. however, the detection of this pathogen in dogs, foxes and golden jackals well outside the areas inhabited by this tick species reinforced the hypothesis that additional ixodids are involved in the life cycle and transmission of this protozoon. the present study provides, for the first time, data supporting the sporogonic development of h. canis in specimens ...201627998321
"candidatus rickettsia asemboensis" in rhipicephalus sanguineus ticks, brazil."candidatus rickettsia asemboensis" is an obligate intracellular bacterium of the rickettsiales order, genetically related to species belonging to the rickettsia felis group, agents of flea-borne spotted fever. here we report for the first time the detection of "ca. r. asemboensis", a flea-associated organism, in rhipicephalus sanguineus ticks. it is the first occurrence of this emerging bacterium in brazil, which increases the geographical distribution of this r. felis-like agent.201727986544
serosurvey of borrelia in dogs, horses, and humans exposed to ticks in a rural settlement of southern brazil.the aims of the present study were to serosurvey dogs, horses, and humans highly exposed to tick bites for anti-borrelia burgdorferi s.l. antibodies, identify tick species present, and determine risk factors associated with seropositivity in a rural settlement of paraná state, southern brazil. eighty-seven residents were sampled, along with their 83 dogs and 18 horses, and individual questionnaires were administered. immunofluorescence antibody test (ifat) was performed on serum samples and posi ...201627982299
molecular detection and characterization of zoonotic and veterinary pathogens in ticks from northeastern china.tick-borne diseases are considered as emerging infectious diseases in humans and animals in china. in this study, ixodes persulcatus (n = 1699), haemaphysalis concinna (n = 412), haemaphysalis longicornis (n = 390), dermacentor nuttalli (n = 253), and dermacentor silvarum (n = 204) ticks were collected by flagging from northeastern china, and detected for infection with anaplasma, ehrlichia, babesia, and hepatozoon spp. by using nested polymerase chain reaction assays and sequencing analysis. an ...201627965644
anaplasma species of veterinary importance in japan.anaplasma species of the family anaplasmataceae, order rickettsiales are tick-borne organisms that can cause disease in animals and humans. in japan, all recognized species of anaplasma (except for anaplasma ovis) and a potentially novel anaplasma sp. closely related to anaplasma phagocytophilum have been reported. most of these detected tick-borne pathogens are believed to be lowly pathogenic in animals in japan although the zoonotic a. phagocytophilum has recently been reported to cause clinic ...201627956767
exon-intron structure and sequence variation of the calreticulin gene among rhipicephalus sanguineus group ticks.calreticulin proteins (crts) are important components of tick saliva, which is involved in the blood meal success, pathogen transmission and host allergic responses. the characterization of the genes encoding for salivary proteins, such as crts, is pivotal to understand the mechanisms of tick-host interaction during blood meal and to develop tick control strategies based on their inhibition. in hard ticks, crt genes were shown to have only one intron with conserved position among species. in thi ...201627955695
detection and molecular identification of hepatozoon canis and babesia vogeli from domestic dogs in palestine.dogs serve as hosts for a great number of parasites, which may affect their health and wellbeing. this study aimed to observe tick borne pathogens in dogs from palestine including hepatozoon canis and babesia species. the prevalence of both h. canis and babesia species infections in apparently healthy dogs, from ten districts of the west bank was surveyed. dna was extracted from blood samples obtained from dogs (n = 362) and ticks (n = 213) collected from dogs (n = 77). a primer set that amplifi ...201727938422
effect of the saliva from different triatomine species on the biology and immunity of tlr-4 ligand and trypanosoma cruzi-stimulated dendritic cells.triatomines are blood-sucking vectors of trypanosoma cruzi, the causative agent of chagas disease. during feeding, triatomines surpass the skin host response through biomolecules present in their saliva. dendritic cells (dcs) play a crucial role in the induction of the protection to aggressive agents, including blood-sucking arthropods. here, we evaluated if salivary components of triatomines from different genera evade the host immunity by modulating the biology and the function of lps- or t. c ...201627938380
laboratory and field evaluation of brown dog tick behavioral responses to potential semiochemicals.the brown dog tick, rhipicephalus sanguineus sensu lato (parasitiformis: ixodidae), is an ectoparasite of dogs that can be found worldwide. this tick poses unique difficulties in management because it can complete its entire life cycle indoors and has demonstrated acaricide resistance. the ability to monitor for tick presence and abundance is necessary for developing effective control programs. as such, an evaluation of adult brown dog tick behavioral responses to 16 potential semiochemicals was ...201727914935
a comparative laboratory trial evaluating the immediate efficacy of fluralaner, afoxolaner, sarolaner and imidacloprid + permethrin against adult rhipicephalus sanguineus (sensu lato) ticks attached to dogs.acaricides are used to treat and prevent tick infestations, and a common clinical scenario is to administer an acaricide on observing an attached tick. consequently, immediate acaricidal efficacy (onset of activity and speed of kill) results are clinically valuable. this study evaluated the immediate efficacy of four commercially available acaricides against adult rhipicephalus sanguineus (sensu lato).201627912787
anaplasma platys in dogs from uruguay.anaplasmataceae family members include vector-borne bacteria of veterinary importance that may also affect humans. ehrlichia canis and anaplasma platys are the main members of this family detected in dogs worldwide. in uruguay there are not many published studies on tick-borne pathogens affecting dogs, the only haemoparasite molecularly confirmed in dogs, is the piroplasm rangelia vitalii. the aim of the present work was to detect the presence of a. platys and e. canis in dogs and dogs-associate ...201727908772
gray wolf exposure to emerging vector-borne diseases in wisconsin with comparison to domestic dogs and humans.world-wide concern over emerging vector-borne diseases has increased in recent years for both animal and human health. in the united sates, concern about vector-borne diseases in canines has focused on lyme disease, anaplasmosis, ehrlichiosis, and heartworm which infect domestic and wild canids. of these diseases, lyme and anaplasmosis are also frequently diagnosed in humans. gray wolves (canis lupus) recolonized wisconsin in the 1970s, and we evaluated their temporal and geographic patterns of ...201627898670
notes from the field: community-based prevention of rocky mountain spotted fever - sonora, mexico, 2016.rocky mountain spotted fever (rmsf), a life-threatening tickborne zoonosis caused by rickettsia rickettsii, is a reemerging disease in mexico (1,2). r. rickettsii is an intracellular bacterium that infects vascular endothelium and can cause multisystem organ failure and death in the absence of timely administration of a tetracycline-class antibiotic, typically doxycycline. epidemic rmsf, as described in parts of arizona and mexico, is associated with massive local infestations of the brown dog t ...201627880753
dinotefuran-induced morphophysiological changes in semi-engorged females rhipicephalus sanguineus latreille, 1806 (acari: ixodidae) ticks: ultra-structural evaluation.the present study demonstrated the effects of dinotefuran (active ingredient of the acaricide protetor pet(®)) on the ovary and midgut cells of semi engorged r. sanguineus females exposed to different concentrations of this chemical. for this, 120 semi-engorged females were divided into four treatment groups with 30 individuals each: group i or control (distilled water), group ii (5000ppm), groups iii (6250ppm) and group iv (8334ppm of dinotefuran). all the ticks were immersed in the different c ...201727876644
first report of amitraz and cypermethrin resistance in rhipicephalus sanguineus sensu lato infesting dogs in mexico.engorged female rhipicephalus sanguineus sensu lato (ixodida: ixodidae) were collected from dogs in the state of yucatán, mexico. fourteen tick populations were collected from dogs at seven veterinary clinics, four residential homes and three cattle farms. the larval immersion test was used in the progeny of collected adult females to test susceptibility to amitraz and cypermethrin. dose-mortality regressions, 50% lethal concentrations (lc50 ), confidence intervals and slope were estimated by pr ...201727859488
morphological and molecular identification of the brown dog tick rhipicephalus sanguineus and the camel tick hyalomma dromedarii (acari: ixodidae) vectors of rickettsioses in egypt.rickettsioses have an epidemiological importance that includes pathogens, vectors, and hosts. the dog tick rhipicephalus sanguineus and the camel tick hyalomma dromedarii play important roles as vectors and reservoirs of rickettsiae. the aim of this study was to determine the prevalence of rickettsiae in ixodid ticks species infesting dogs and camels in egypt, in addition to, the morphological and molecular identification of r. sanguineus and h. dromedarii.201627847418
ticks (acari: ixodidae) infesting cattle and some other domestic and wild hosts on the french mediterranean island of corsica.corsica is a mountainous french island in the north-western mediterranean presenting a large diversity of natural environments where many interactions between domestic animals and wild fauna occur. despite a favourable context for ticks and tick-borne diseases (tbds), the tick fauna of corsica has not systematically been investigated.201627842608
cercopithifilaria sp. ii in vulpes vulpes: new host affiliation for an enigmatic canine filarioid.cercopithifilaria bainae and cercopithifilaria grassii (spirurida, onchocercidae) are filarioids inhabiting the skin of dogs worldwide. the microfilariae of a third species namely, cercopithifilaria sp. ii sensu otranto et al. 2013, have been morphologically and molecularly characterized but scientific knowledge of this parasite is minimal. the first case of infection of a red fox (vulpes vulpes) with the filarioid cercopithifilaria sp. ii is herein described in castro marim, portugal. microfila ...201727838834
the role of cc-chemokines in the regulation of angiogenesis.angiogenesis, the formation of new blood vessels, is critical for survival and in the regenerative response to tissue injury or ischemia. however, in diseases such as cancer and atherosclerosis, inflammation can cause unregulated angiogenesis leading to excessive neovascularization, which exacerbates disease. current anti-angiogenic therapies cause complete inhibition of both inflammatory and ischemia driven angiogenesis causing a range of side effects in patients. specific inhibition of inflamm ...201627834814
small regulatory rnas of rickettsia conorii.small regulatory rnas comprise critically important modulators of gene expression in bacteria, yet very little is known about their prevalence and functions in rickettsia species. r. conorii, the causative agent of mediterranean spotted fever, is a tick-borne pathogen that primarily infects microvascular endothelium in humans. we have determined the transcriptional landscape of r. conorii during infection of human microvascular endothelial cells (hmecs) by strand-specific rna sequencing to ident ...201627834404
ferritin 1 silencing effect in rhipicephalus sanguineus sensu lato (acari: ixodidae) during experimental infection with ehrlichia canis.rhipicephalus sanguineus sensu lato (s.l.) is a very common ectoparasite of domestic dogs able to transmit several pathogens of human and veterinary importance. tick infestations and tick-borne diseases (tbds) remain a serious and persistent problem, due to the lack of efficient control measures. it is therefore vital that novel approaches to control are pursued. whilst vaccination is recognised as a potential control method to reduce tick infestation, no anti-r. sanguineus vaccine is available. ...201727825659
tick (acari: ixodidae) infestations in cattle along geba river basin in guinea-bissau.tick infestations are a major problem for animal production in tropical areas where prevention and control remain deficient. the present study sought to assess the awareness of traditional cattle producers towards the importance of ticks and aimed at the identification of tick species infesting bovines within the geba river basin, guinea-bissau. interviews with producers revealed that the majority directly correlates the presence of ticks with the occurrence of diseases in cattle. however, insuf ...201727825658
species composition, distribution, ecological preference and host association of ticks in cyprus.tick population and species depend on the effect of biotic and abiotic factors, especially vegetation, climate and host density; cyprus, due to the mild climate, favors the appearance and spread of tick-borne infections. our objective was to identify the tick species present in the island, to investigate their geographical distribution and their epidemiological implications. during a three-year study (2004-2006) we collected ticks from domestic and wild animals over the island of cyprus. data on ...201627822784
efficacy of tagetes minuta (asteraceae) essential oil against rhipicephalus sanguineus (acari: ixodidae) on infested dogs and in vitro.ticks from rhipicephalus sanguineus complex are widely distributed in the world and one species from this complex is the most common tick on dogs in brazil, notably in urban areas. this tick is a vector of several diseases. among others it transmits the agent of canine ehrlichiosis, a major dog infectious disease and the agent of rocky mountain spotted fever. this tick can spread rapidly and develop intolerable infestations within no time. currently tick control is done with acaricides and deman ...027815656
molecular analysis of rhipicephalus sanguineus (acari: ixodidae), an incriminated vector tick for babesia vogeli in taiwan.the genetic identity of rhipicephalus sanguineus tick was determined for the first time in taiwan. the phylogenetic relationships were analyzed by comparing the sequences of mitochondrial 16s ribosomal dna gene obtained from 32 strains of ticks representing six species of rhipicephalus, two species of dermacentor and two outgroup species (haemaphysalis inermis and ixodes ricinus). seven major clades can be easily distinguished by neighbour-joining analysis and were congruent by maximum-parsimony ...201627815655
rhipicephalus sanguineus (acari: ixodidae) species complex: morphometric and ultrastructural analyses.although some studies have already been performed on rhipicephalus sanguineus (latreille) populations, there is still much disarray around its taxonomic classification. rhipicephalus sanguineus, which is also known as " (brown dog tick) is a three-host tick that parasitizes many vertebrates, including occasional human hosts. it presents a worldwide distribution and is a vector of zoonosis with acknowledged public health importance. in western europe, r. turanicus (pomerantsev) share many phenoty ...201627812830
host body size and the diversity of tick assemblages on neotropical vertebrates.identifying the factors that influence the species diversity and distribution of ticks (acari: ixodida) across vertebrate host taxa is of fundamental ecological and medical importance. host body size is considered one of the most important determinants of tick abundance, with larger hosts having higher tick burdens. the species diversity of tick assemblages should also be greater on larger-bodied host species, but empirical studies testing this hypothesis are lacking. here, we evaluate this rela ...201627812506
parasites and vector-borne diseases in client-owned dogs in albania: infestation with arthropod ectoparasites.to establish the diversity and seasonality of ectoparasite infestation in client-owned dogs in albania, 602 dogs visiting four small animal clinics in tirana from march 2010 to april 2011 inclusive were examined for ectoparasites by full body search and total body comb. in addition, ear swab specimens collected from all dogs and scrapings taken from skin lesions suspicious of mite infestation were examined for parasitic mites. overall, 93 dogs (15.4 %, 95%ci 12.6-18.6) were demonstrated to be in ...201727796564
confirmation of occurrence of <i>babesia vogeli</i> in a dog in windhoek, central namibia.although there is evidence of high seroprevalence of antibodies to babesia spp. in dogs in central namibia, clinical babesiosis is rarely diagnosed. rhipicephalus sanguineus sensu lato, the vector of babesia vogeli, is common in namibia while haemaphysalis elliptica, the vector of the highly virulent but morphologically indistinguishable babesia rossi, has rarely been recorded, mainly in northern namibia. on the basis of vector occurrence, clinical cases of canine babesiosis in windhoek, central ...201627796110
comparative biology of the tropical and temperate species of rhipicephalus sanguineus sensu lato (acari: ixodidae) under different laboratory conditions.recent studies have shown that the taxon rhipicephalus sanguineus sensu lato (s.l.) is represented in latin america by two distinct species, designated as 'tropical species' (distributed from mexico to brazil) and 'temperate species' (restricted to the southern cone of south america). since both tropical and temperate species of r. sanguineus s.l. are parasites primarily of domestic dogs, the reasons for their distinct geographical distribution in south america could be related to particular req ...201727793613
ixodid ticks in the megapolis of kyiv, ukraine.the ixodidae include the most common tick species encountered in europe. the ticks transmit a variety of bacterial and protozoan agents of medical and veterinary significance. the aim of the current work was to investigate distribution of ixodes ricinus and dermacentor reticulatus ticks in kyiv, the largest and most densely populated megapolis of ukraine. ticks were collected at various recreational areas by flagging during may, the month that showed the highest tick abundance in the past. sex d ...201727771333
spotted fever group rickettsia in the pampa biome, brazil, 2015-2016. 027767913
rangelia vitalii in a free-ranging maned wolf (chrysocyon brachyurus) and co-infections.an adult free-ranged female maned wolf was rescued from a periurban area subject to anthropogenic disturbances in the minas gerais, brazil. the animal presented poor body condition and anemia. the clinical condition rapidly deteriorated culminating in dead and a necropsy was performed. the main gross lesions were marked anemia and blood content in the intestines accompanied by many types of parasites. the protozoa rangelia vitalii was identified by histopathological analysis predominantly within ...201627761403
safety evaluation of parastar(®) plus in dogs and assessment of transferable residue of fipronil and cyphenothrin from dogs to humans.dogs are easily infested with fleas, ticks, and other ectoparasites serving as vectors for transmitting bacterial, viral, and parasitic diseases. therefore, the use of ectoparasiticides is inevitable and important. the present investigation was undertaken with two specific objectives: one, to evaluate the safety of fipronil and cyphenothrin in dogs after topical application of parastar(®) plus, and two, to determine the transferable residue of these insecticides from dogs to humans. six healthy, ...201627747219
identification, distribution and population dynamics of francisella-like endosymbiont in haemaphysalis doenitzi (acari: ixodidae).francisella-like endosymbionts (fles) with significant homology to francisella tularensis (γ-proteobacteria) have been characterized in several tick species, whereas knowledge on their distribution and population dynamics in ticks remains meager. hence, in the current study, we identified a novel francisella-like endosymbiont (fles-hd) from the tick haemaphysalis doenitzi and evaluated the putative functions of this symbiont. results indicated that fles-hd had 100% infection rate and a perfect v ...201627731377
heat-stressed metarhizium anisopliae: viability (in vitro) and virulence (in vivo) assessments against the tick rhipicephalus sanguineus.the current study investigated the thermotolerance of metarhizium anisopliae s.l. conidia from the commercial products metarril® sp organic and metarril® wp. the efficacy of these m. anisopliae formulations against the tick rhipicephalus sanguineus s.l. was studied in laboratory under optimum or heat-stress conditions. the products were prepared in water [tween® 80, 0.01 % (v/v)] or pure mineral oil. conidia from metarril® sp organic suspended in water presented markedly delayed germination afte ...201727704216
canine and ovine tick-borne pathogens in camels, nigeria.in april 2008, whole blood samples were collected from 36 dromedary camels in sokoto, north-western nigeria. following pcr and reverse line blotting, twenty-two samples (61%) resulted positive for ehrlichia/anaplasma spp. and three (8%) for theileria/babesia spp., with three (8%) cases of co-infections being found. both sequence and blast analyses identified ehrlichia/anaplasma spp. and theileria/babesia spp. positive cases as anaplasma platys and theileria ovis, respectively. this is the first ...201627692338
ectoparasites of free-roaming domestic cats in the central united states.free-roaming domestic cat (felis catus) populations serve as a valuable resource for studying ectoparasite prevalence. while they share a similar environment as owned cats, free-roaming cats do not receive routine veterinary care or ectoparasiticide application, giving insight into parasite risks for owned animals. we examined up to 673 infested cats presented to a trap-neuter-return (tnr) clinic in the central united states. ectoparasite prevalences on cats were as follows: fleas (71.6%), ticks ...201627692321
evaluation of pharmacokinetics and efficacy of ivermectin following oral administration in dogs against experimental infection of ctenocephalides felis felis and rhipicephalus sanguineus.with the increasing number of pets in home the human-animal relationship is increasingly close and care about control disease growing. ivermectin (ivm) is frequently used because its proven safety. ivm is recommended for the treatment of demodectic scabies and prevention of heartworm in dogs, but informally is extremely used to control of ctenocephalides felis felis and rhipicephalus sanguineus. the aim of this study is evaluate the use of ivm in dogs, by the oral route at 0.6μg/kg dose, against ...201627692320
first detection and molecular identification of babesia gibsoni from rhipicephalus sanguineus ticks.babesia gibsoni was firstly identified in rhipicephalus sanguineus ticks infested on dogs in asia. a total of 1195 ticks collected from veterinary practitioners and pet clinics were examined for babesia by nested-polymerase chain reaction assay based on the 18s small subunit ribosomal rna (ssrrna) gene. babesia infection was detected in nymph, male and female ticks with an infection rate of 2.42%, 0.98% and 1.97%, respectively. sequence and phylogenetic analysis revealed that these babesia spp. ...201727686959
bacterial pathogens and endosymbionts in ticks.ticks collected from goats in northern greece were tested for the presence of tick-borne bacteria. among adult ticks, 37 (57.8%) were rhipicephalus bursa, 11 (17.2%) dermacentor marginatus, 10 (15.6%) ixodes ricinus, 3 (4.7%) rhipicephalus sanguineus sensu lato and 2 (3.1%) haemaphysalis parva; one (1.6%) rhipicephalus spp. tick was nymph. rickettsia monacensis, rickettsia massilae, anaplasma phagocytophilum and anaplasma platys were detected in i. ricinus and rh. bursa ticks. a variety of coxie ...201727686386
proceedings of the ix international symposium on phlebotomine sandflies (isops ix), reims, france, june 28th-july 1st, 2016. 201627677701
species diversity and spatial distribution of ixodid ticks on small ruminants in greece.a cross sectional field study on the distribution of ixodidae ticks was carried out over two consecutive tick seasons (2012 and 2013) in small ruminants (309 farms) located in mainland and five islands of greece. a total of 2108 ticks (1199 females, 908 males, and 1 nymph) were collected from sheep and goats. two species of rhipicephalus, two of ixodes, five of hyalomma, three of haemaphysalis, and one of dermacentor were detected. the species with the widest distribution were rhipicephalus sang ...201627655133
manual for maintenance of multi-host ixodid ticks in the laboratory.use of laboratory animals as hosts for blood-sucking arthropods remains a time-proven and the most efficient method for establishment and propagation of slowly feeding ixodid ticks, despite introduction of techniques involving artificial feeding on either animal skins or synthetic membranes. new zealand white rabbits are usually the most accessible and most suitable hosts routinely used for establishment and maintenance of a large variety of multi-host tick species. here we describe standard pro ...201627651325
kampinos national park: a risk area for spotted fever group rickettsioses, central poland?ixodid ticks are important vectors of a variety of bacterial and protozoan pathogens which cause infections in humans. in this study, altogether 1041 questing ixodes ricinus (n = 305) and dermacentor reticulatus ticks (n = 736), sympatrically occurring in kampinos national park (kpn), central-east poland, were analyzed by pcr for rickettsia species. overall, the pathogen prevalence in ticks was 27.5 % for i. ricinus and 42.8 % for d. reticulatus. sequencing analysis showed that the first tick sp ...201627631765
status of tick infections among semi-wild cattle in arunachal pradesh, india.to explore the seasonal prevalence of tick infections in mithun (bos frontalis) and yak (b. grunniens) a study was conducted in papum pare district, kurung-kumey district and west kameng district of arunachal pradesh, india from december 2012 to november 2014. adult ticks were collected from both the animals during the month of december 2012 to november 2014. ectoparasites were identified on the basis of their morpho-anatomical features observed through light microscope. a total of nine differen ...201727614479
prevalence of ixodid ticks on cattle and sheep northeast of iran.a survey was carried out to investigate the prevalence of hard tick species (acari: ixodidae) on cattle and sheep north of iran. the aim of study was to determine the prevalence of hard ticks on cattle and sheep in the mountainous areas of golestan province and their geographical distribution. a total of 26 ticks were collected from 22 infested cattle and 26 ticks were collected from 12 infested sheep during activating seasons of ticks in 2013-2014. the species collected from cattle and sheep we ...201627605782
coxiella symbionts are widespread into hard ticks.ticks are blood-feeding arthropods and can harbor several bacteria, including the worldwide zoonotic disease q-fever agent coxiella burnetii. recent studies have reported a distinct group of coxiella mostly associated with ixodidae ticks, including the primary endosymbionts of amblyomma americanum. in the present work, a screening for coxiella infection was performed by 16s ribosomal dna (rdna) gene analyses in 293 tick samples of 15 different species sampled worldwide, including brazil, colombi ...201627595990
detection of rickettsiae in fleas and ticks from areas of costa rica with history of spotted fever group rickettsioses.outbreaks of spotted fevers have been reported in costa rica since the 1950s, although vectors responsible for transmission to humans have not been directly identified. in this study, species of rickettsia were detected in ectoparasites from costa rica, mostly from five study sites where cases of spotted fevers have been reported. ticks and fleas were collected using drag cloths or directly from domestic and wild animals and pooled according to species, host, and location. pools were analyzed in ...201627592065
induction of humoral immune response to multiple recombinant rhipicephalus appendiculatus antigens and their effect on tick feeding success and pathogen transmission.rhipicephalus appendiculatus is the primary vector of theileria parva, the etiological agent of east coast fever (ecf), a devastating disease of cattle in sub-saharan africa. we hypothesized that a vaccine targeting tick proteins that are involved in attachment and feeding might affect feeding success and possibly reduce tick-borne transmission of t. parva. here we report the evaluation of a multivalent vaccine cocktail of tick antigens for their ability to reduce r. appendiculatus feeding succe ...201627589998
occurrence of mycoplasma haemocanis in dogs infested by ticks in campo grande, mato grosso do sul, brazil.hemotropic mycoplasmas in dogs, such as mycoplasma haemocanis, have been described worldwide. recently, these pathogens have been reported to be causative agent of zoonosis. it is known that its transmission may occur through the action of blood-sucking arthropods (e.g. ticks or fleas), through blood transfusion, contaminated fomites and/or transplacentally. in brazil, m. haemocanis is present in practically all regions and the tick rhipicephalus sanguineus sensu lato is suspected the main vecto ...201627580391
the intracellular bacterium anaplasma phagocytophilum selectively manipulates the levels of vertebrate host proteins in the tick vector ixodes scapularis.the intracellular bacteria anaplasma phagocytophilum are emerging zoonotic pathogens affecting human and animal health, and a good model for the study of tick-host-pathogen interactions. this tick-borne pathogen is transmitted by ixodes scapularis in the united states where it causes human granulocytic anaplasmosis. tick midguts and salivary glands play a major role during tick feeding and development, and in pathogen acquisition, multiplication and transmission. vertebrate host proteins are fou ...201627561965
an update on the epidemiological situation of spotted fever in brazil.spotted fever is a tick-borne rickettsial disease. in brazil, its notification to the ministry of health is compulsory. since 2007, cases of spotted fever have been integrated to the notifiable diseases information system, and epidemiological analyzes are part of the routines on surveillance programs.201627555867
molecular evidence of bartonella species in ixodid ticks and domestic animals in palestine.ticks play an important role in disease transmission as vectors for human and animal pathogens, including the gram-negative pathogen bartonella. here, we evaluated the presence of bartonella in ixodid ticks and domestic animals from palestine. we tested 633 partly engorged ticks and 139 blood samples from domestic animals (dogs, sheep and camels) for bartonella using its-pcr. bartonella dna was detected in 3.9% of the tested ticks. none of the ticks collected from sheep and goats were positive f ...201627540374
fleas and ticks in carnivores from a domestic-wildlife interface: implications for public health and wildlife.fleas and ticks are parasites of wild and domestic mammals, and can be vectors of several pathogens. in rural areas, domestic carnivores such as the domestic dog (canis lupus familiaris l.), may act as a "bridge" between natural areas and human settlements where ectoparasites can be used as a metric of such link. the aim of this study was to identify fleas, ticks, and rickettsia spp., collected from domestic and wild carnivores in a natural reserve and surrounding human settlements in central ch ...201627539151
new records of ticks (acari: ixodidae) from dogs, cats, humans, and some wild vertebrates in alaska: invasion potential.during 2010-2016, tick specimens were solicited from veterinarians, biologists, and members of the public in alaska. eight species of ticks were recorded from domestic dogs. some ticks were collected from dogs with recent travel histories to other countries or other u.s. states, which appears to explain records of ticks not native to alaska such as amblyomma americanum (l.) (lone star tick), ixodes scapularis (say) (blacklegged tick), and ixodes ricinus (l.). however, we recorded dermacentor var ...201627524823
a novel ehrlichial agent detected in tick in french polynesia.ticks are hematophageous arthropods that are known to host and transmit miscellaneous pathogens including zoonotic bacteria. the aim of this study was to investigate the presence of tick-associated microorganisms in tahiti, french polynesia with molecular tools. a total of 658 ticks from two species including rhipicephalus sanguineus s.l. and rh. annulatus were collected with forceps on dogs and cattle respectively, or with a flag on pasture in several locations of tahiti in 2013. two rickettsia ...201627522945
effect of amblyomma maculatum (acari: ixodidae) saliva on the acute cutaneous immune response to rickettsia parkeri infection in a murine model.rickettsia parkeri luckman (rickettsiales: rickettsiaceae) is a pathogenic spotted fever group rickettsia transmitted by amblyomma maculatum koch (acari: ixodidae) in the united states. the acute innate immune response to this pathogen and the effect of tick feeding or salivary components on this response is largely unknown. we hypothesized that a. maculatum saliva enhances r. parkeri infection via downregulation of the acute cellular and cytokine immune response. c3h/hen mice were intradermally ...201627521760
temporal and spatial distribution and species diversity of hard ticks (acari: ixodidae) in the eastern region of caspian sea.ticks are important parasites because of their voracious blood-feeding activity, and being as vectors for various agents of diseases in both human and livestock. this study was conducted in a monthly schedule from october 2014 to december 2015, at 45 study sites in three regions bordering the caspian sea, according to the topography including hillside, plain and coastal areas in collecting ticks from the body of sheep. alfa and beta biodiversity indices were calculated and compared between the t ...201627519473
systemically and cutaneously distributed ectoparasiticides: a review of the efficacy against ticks and fleas on dogs.acaricidal (tick) and insecticidal (flea) efficacy of systemically and cutaneously distributed ectoparasiticide products for dogs are compared based on permethrin and fluralaner as representative molecules. results of efficacy studies against fleas and ticks are reviewed that show generally good to excellent results. both externally and systemically distributed treatments have benefits and weaknesses in potentially preventing pathogen transmission by these arthropod vectors.four general properti ...201627502490
natural transovarial and transstadial transmission of leishmania infantum by naïve rhipicephalus sanguineus ticks blood feeding on an endemically infected dog in shiraz, south of iran.the visceral leishmaniasis parasite, leishmania infantum, is naturally transmitted through the bites of phlebotomine sand flies. alternative routes of transmission are questioned. the main aim is to verify the passage of l. infantum kdna in ticks, rhipicephalus sanguineus, blood feeding on a parasitemic dog in shiraz, south of iran.201627496515
identification of a novel β-adrenergic octopamine receptor-like gene (βaor-like) and increased atp-binding cassette b10 (abcb10) expression in a rhipicephalus microplus cell line derived from acaricide-resistant ticks.the cattle tick rhipicephalus (boophilus) microplus is an economically important parasite of livestock. effective control of ticks using acaricides is threatened by the emergence of resistance to many existing compounds. several continuous r. microplus cell lines have been established and provide an under-utilised resource for studies into acaricide targets and potential genetic mutations associated with resistance. as a first step to genetic studies using these resources, this study aimed to de ...201627484910
canine babesiosis and tick activity monitored using companion animal electronic health records in the uk.recent publications highlighting autochthonous babesia canis infection in dogs from essex that have not travelled outside the uk are a powerful reminder of the potential for pathogen emergence in new populations. here the authors use electronic health data collected from two diagnostic laboratories and a network of 392 veterinary premises to describe canine babesia cases and levels of babesia concern from january 2015 to march 2016, and the activity of ticks during december 2015-march 2016. in m ...201627484328
molecular detection and identification of rickettsiales pathogens in dog ticks from costa rica.although vector-borne diseases are globally widespread with considerable impact on animal production and on public health, few reports document their presence in central america. this study focuses on the detection and molecular identification of species belonging to selected bacterial genera (ehrlichia, anaplasma and rickettsia) in ticks sampled from dogs in costa rica by targeting several genes: 16s rrna/dsb genes for ehrlichia; 16s rrna/groel genes for anaplasma, and ompa/glta/groel genes for ...201627480409
rickettsia (rickettsiales: rickettsiaceae) vector biodiversity in high altitude atlantic forest fragments within a semiarid climate: a new endemic area of spotted-fever in brazil.rickettsioses are re-emerging vector-borne zoonoses with a global distribution. recently, rickettsia sp. strain atlantic rainforest has been associated with new human spotted-fever (sf) cases in brazil, featuring particular clinical signs: eschar formation and lymphadenopathy. these cases have been associated with the tick species, amblyomma ovale from 2010 until 2015, the brazilian health department confirmed 11 human sf cases in the maciço de baturité region, ceará, brazil. the present study r ...201627480099
artisanal extraction and traditional knowledge associated with medicinal use of crabwood oil (carapa guianensis aublet.) in a peri-urban várzea environment in the amazon estuary.várzea forests of the amazon estuary contain species of importance to riverine communities. for example, the oil extracted from the seeds of crabwood trees is traditionally used to combat various illnesses and as such artisanal extraction processes have been maintained. the objectives of this study were to (1) describe the process involved in artisanal extraction of crabwood oil in the fazendinha protected area, in the state of amapá; (2) characterise the processes of knowledge transfer associat ...201627478479
countrywide serological evaluation of canine prevalence for anaplasma spp., borrelia burgdorferi (sensu lato), dirofilaria immitis and ehrlichia canis in mexico.canine vector-borne diseases (cvbd) have become a major concern for canine and human public health. the aim of the study described here is to add epidemiological data regarding four pathogens responsible for cvbd, namely anaplasmosis, borreliosis, dirofilariosis and ehrlichiosis in a national survey conducted in mexico.201627474020
autochthonous hepatozoon infection in hunting dogs and foxes from the czech republic.blood samples from 21 red foxes (vulpes vulpes) and 8 hunting dogs from the same locality in the czech republic were examined for presence of hepatozoon canis/hepatozoon sp. the dogs were selected based on their close contact with foxes during fox bolting and because they had not traveled into known endemic areas. using diagnostic pcr amplifying partial 18s rdna fragment, hepatozoon dna was detected in 20 red foxes (95 %) and 4 dogs (50 %). from 8 positive foxes and 2 positive dogs, we obtained ...201627431805
comparative speed of kill of sarolaner (simparica™ chewables) and fluralaner (bravecto(®)) against induced infestations of amblyomma americanum on dogs.the lone star tick, amblyomma americanum, infests dogs and cats in north america and transmits the pathogens ehrlichia chaffeensis and ehrlichia ewingii, which cause monocytic and granulocytic ehrlichiosis in dogs and humans, and cytauxzoon felis which causes cytauxzoonosis in cats. a parasiticide's speed of kill is important to minimize the direct deleterious effects [related to blood-feeding] of tick infestation and reduce the risk of transmission of tick-borne pathogens. in this study the spe ...201627430425
rickettsial disease in the peruvian amazon basin.using a large, passive, clinic-based surveillance program in iquitos, peru, we characterized the prevalence of rickettsial infections among undifferentiated febrile cases and obtained evidence of pathogen transmission in potential domestic reservoir contacts and their ectoparasites. blood specimens from humans and animals were assayed for spotted fever group rickettsiae (sfgr) and typhus group rickettsiae (tgr) by elisa and/or pcr; ectoparasites were screened by pcr. logistic regression was used ...201627416029
prevalence of ectoparasites in owned dogs in kerman city, southeast of iran.the present study aimed to investigate the intensity and distribution of natural infestation of ectoparasites in owned dogs during june to november 2011 in kerman city, southeast of iran. one hundred two domestic dogs (63 males, 39 females) with age ranged between 45 days to 13 years were examined for ectoparasites infestation including (tick, lice, flea and mite) in kerman city. detailed questionnaire about grooming and bathing interval, keeping place (outdoor, indoor) and purpose of keeping we ...201627413320
vector databank in the indian armed forces.medical intelligence of disease vectors deals with understanding vector distribution and control.201127408077
anaplasma marginale and theileria annulata in questing ticks from portugal.ticks are ubiquitous arthropods and vectors of several pathogenic agents in animals and humans. monitoring questing ticks is of great importance to ascertain the occurrence of pathogens and the potential vector species, offering an insight into the risk of disease transmission in a given area. in this study 428 host-seeking ticks, belonging to nine species of ixodidae and collected from 17 of the 23 portuguese mainland subregions, were screened for several tick-borne agents with veterinary relev ...201627394441
repellency to ticks (acari: ixodidae) of extracts of nigella sativa (ranunculaceae) and the anti-inflammatory dogsbestfriend™.motivated by observations that the canine anti-inflammatory cream dogsbestfriend™ (dbf) appeared to deter flies, mosquitoes, and ticks from treated animals, repellent efficacy bioassays using four species of ticks were conducted with three extracts of nigella sativa l. (ranunculaceae), a constituent of dbf. the dbf cream was tested against nymphs of lone star tick, amblyomma americanum (l.). in vertical filter paper assays, the three extracts applied at 0.413 mg extract/cm(2) filter paper repell ...201627394440
prevalence and infection intensity of rickettsia massiliae in rhipicephalus sanguineus sensu lato ticks from mendoza, argentina.rickettsia massiliae belongs to the spotted fever group and in the new world is commonly associated with the brown dog tick, rhipicephalus sanguineus. herein we investigate the presence of r. massiliae in rh. sanguineus sensu lato ticks in a location near the andean foothills (mendoza, argentina), to provide a prevalence estimate and to assess the infection intensity of this pathogen. rickettsia massiliae infection was found in 5.1% of the rh. sanguineus s.l ticks analyzed, all with high infecti ...201627394145
synergism of thymol, carvacrol and eugenol in larvae of the cattle tick, rhipicephalus microplus, and brown dog tick, rhipicephalus sanguineus.the effects of combinations of the monoterpenes thymol and carvacrol and the phenylpropanoid eugenol in larvae of rhipicephalus microplus (canestrini, 1888) (acari: ixodidae) and rhipicephalus sanguineus sensu lato (s.l.) (acari: ixodidae) were assessed by the larval packet test. the compusyn program was used to make qualitative assessments of the effects (synergistic, additive and antagonistic) of the associations. the effects of all combinations tested against r. microplus larvae were synergis ...201627392803
ticks infesting domestic dogs in the uk: a large-scale surveillance programme.recent changes in the distribution of tick vectors and the incidence of tick-borne disease, driven variously by factors such as climate change, habitat modification, increasing host abundance and the increased movement of people and animals, highlight the importance of ongoing, active surveillance. this paper documents the results of a large-scale survey of tick abundance on dogs presented to veterinary practices in the uk, using a participatory approach that allows relatively cost- and time-eff ...201627388169
diversity and distribution of tick species (acari: ixodidae) associated with human otoacariasis and socio-ecological risk factors of tick infestations in sri lanka.tick infestation in humans is a major public health concern. the diversity and distribution of tick species associated with human otoacariasis was studied in five districts: anuradhapura, kandy, kurunegala, nuwara eliya and ratnapura in the main agro-climatic zones of sri lanka. ticks from patients attending the ear, nose and throat clinics of the general hospitals were collected during a 3 year period. in total 426 ticks were collected. most human otoacariasis cases were reported from kandy (33 ...201627382981
crimean-congo hemorrhagic fever virus lineages europe 1 and europe 2 in bulgarian ticks.crimean-congo hemorrhagic fever (cchf) is a tick-borne human viral disease with fatality rate up to 30%. two genetic lineages of cchf virus (cchfv) are present in europe - lineage europe 1 which contains pathogenic cchfv strains, and lineage europe 2 consisting of ap92 and ap92-like strains. in order to investigate cchfv distribution and potential risk for humans, ticks were collected from livestock in the five districts where cchf cases have been reported in the last five years in bulgaria. cch ...201627378409
evasins: therapeutic potential of a new family of chemokine-binding proteins from ticks.blood-sucking parasites, such as ticks, remain attached to their hosts for relatively long periods of time in order to obtain their blood meal without eliciting an immune response. one mechanism used to avoid rejection is the inhibition of the recruitment of immune cells, which can be achieved by a class of chemokine-binding proteins (ckbps) known as evasins. we have identified three distinct evasins produced by the salivary glands of the common brown dog tick, rhipicephalus sanguineus. they dis ...201627375615
molecular detection and characterization of spotted fever group rickettsiae in ticks from central italy.the aim of this study was to investigate the presence of rickettsial pathogens in ticks from central italy. a total of 113 ticks hailed from latium and tuscany regions were identified and tested by pcr to detect glta, ompa, ompb genes of rickettsia. positive amplicons were sequenced and identified at species level. ticks were analyzed individually or in pools. the percentage of positivity for sfg rickettsiae was 12.4%, expressed as minimum infection rate (mir) assuming that one tick was positive ...201627365155
host specificity in a diverse neotropical tick community: an assessment using quantitative network analysis and host phylogeny.host specificity is a fundamental determinant of tick population and pathogen transmission dynamics, and therefore has important implications for human health. tick host specificity is expected to be particularly high in the tropics, where communities of ticks, hosts and pathogens are most diverse. yet the degree to which tropical tick species are host-specific remains poorly understood. combining new field data with published records, we assessed the specificity of tick-host associations in pan ...201627357506
exposure to vector-borne pathogens in candidate blood donor and free-roaming dogs of northeast italy.many vector-borne pathogens including viruses, bacteria, protozoa and nematodes occur in northeast italy, representing a potential threat to animal and human populations. little information is available on the circulation of the above vector-borne pathogens in dogs. this work aims to (i) assess exposure to and circulation of pathogens transmitted to dogs in northeast italy by ticks, sandflies, and mosquitoes, and (ii) drive blood donor screening at the newly established canine blood bank of the ...201627357128
borrelia sp. phylogenetically different from lyme disease- and relapsing fever-related borrelia spp. in amblyomma varanense from python reticulatus.species of the genus borrelia are causative agents of lyme disease and relapsing fever. lyme disease is the most commonly reported vector-borne disease in the northern hemisphere. however, in some parts of the world lyme borreliosis and relapsing fever may be caused by novel borrelia genotypes. herein, we report the presence of a borrelia sp. in an amblyomma varanense collected from python reticulatus.201627342652
rickettsia felis, an emerging flea-borne rickettsiosis.rickettsia felis is an emerging insect-borne rickettsial pathogen and the causative agent of flea-borne spotted fever. first described as a human pathogen from the usa in 1991, r. felis is now identified throughout the world and considered a common cause of fever in africa. the cosmopolitan distribution of this pathogen is credited to the equally widespread occurrence of cat fleas (ctenocephalides felis), the primary vector and reservoir of r. felis. although r. felis is a relatively new member ...201627340613
effect of heat stress and oil formulation on conidial germination of metarhizium anisopliae s.s. on tick cuticle and artificial medium.the effect of heat stress (45°c) versus non-heat stress (27°c) on germination of metarhizium anisopliae sensu stricto (s.s.) isolate ip 119 was examined with conidia formulated (suspended) in pure mineral oil or in water (tween 80, 0.01%), and then applied onto the cuticle of rhipicephalus sanguineus sensu lato (s.l.) engorged females or onto culture medium (pday). in addition, bioassays were performed to investigate the effect of conidia heated while formulated in oil, then applied to blood-eng ...201627317831
comparative efficacy of oral administrated afoxolaner (nexgard™) and fluralaner (bravecto™) with topically applied permethrin/imidacloprid (advantix(®)) against transmission of ehrlichia canis by infected rhipicephalus sanguineus ticks to dogs.the ability of the topical spot-on advantix(®) (50 % permethrin/10 % imidacloprid) to prevent transmission of ehrlichia canis by infected rhipicephalus sanguineus ticks to dogs has previously been reported. the recent market introduction of chewable tablets containing the novel compounds, afoxolaner (nexgard™) and fluralaner (bravecto™) enabled us to conduct a comparative efficacy study with respect to the ability of these three products to block transmission of e. canis by ticks to dogs. the sp ...201627317101
molecular assay on crimean congo hemorrhagic fever virus in ticks (ixodidae) collected from kermanshah province, western iran.crimean-congo hemorrhagic fever (cchf) is a feverous and hemorrhagic disease endemic in some parts of iran and caused by an arbovirus related to bunyaviridae family and nairovirusgenus. the main virus reservoir in the nature is ticks, however small vertebrates and a wide range of domestic and wild animals are regarded as reservoir hosts. this study was conducted to determine the infection rate of cchf virus in hard ticks of sarpole-zahab county, kermanshah province, west of iran.201627308296
ectoparasites of stray dogs in mazandaran, gilan and qazvin provinces, north and center of iran.the aim of the present study was to determine the prevalence of ectoparasite infestations in stray dogs in mazandaran, gilan and qazvin provinces in fall and winter in 2013(december to march).201627308294
ehrlichiosis in household dogs and parasitized ticks in kerman-iran: preliminary zoonotic risk assessment.ehrlichiosis is an emerging tick-borne zoonotic disease caused by the family of anaplasmatacea. recently, outbreak of human monocytic ehrlichiosis was reported in northern part of iran. besides, serological evidence of canine monocytic ehrlichiosis caused by ehrlichia canis was reported from southeastern of iran but the epidemiology of this disease is almost undetermined in iran. the present study was designed to use pcr for detection of ehrlichia spp. in tick infested household dogs and determi ...201627308282
anthelmintic effect of bacillus thuringiensis strains against the gill fish trematode centrocestus formosanus.parasitic agents, such as helminths, are the most important biotic factors affecting aquaculture, and the fluke centrocestus formosanus is considered to be highly pathogenic in various fish species. there have been efforts to control this parasite with chemical helminthicides, but these efforts have had unsuccessful results. we evaluated the anthelmintic effect of 37 strains of bacillus thuringiensis against c. formosanus metacercariae in vitro using two concentrations of total protein, and only ...201627294137
a review of canine babesiosis: the european perspective.canine babesiosis is a significant tick-borne disease caused by various species of the protozoan genus babesia. although it occurs worldwide, data relating to european infections have now been collected for many years. these data have boosted the publication record and increased our working knowledge of these protozoan parasites. both the large and small forms of babesia species (b. canis, b. vogeli, b. gibsoni, and b. microti-like isolates also referred to as "b. vulpes" and "theileria annae") ...201627289223
virulence genes of rickettsia rickettsii are differentially modulated by either temperature upshift or blood-feeding in tick midgut and salivary glands.rickettsia rickettsii, the etiological agent of rocky mountain spotted fever, is transmitted to humans by ticks. during tick feeding, r. rickettsii is exposed to both temperature elevation and components of the blood meal, which have previously been associated with the reactivation of its virulence. these environmental stimuli were also reported to modulate virulence genes of r. rickettsii infecting a set of organs of adult females of its natural vector, amblyomma aureolatum.201627287539
prevalence of vector-borne pathogens in dogs from haiti.canine vector-borne pathogens are common on some caribbean islands, but survey data in haiti are lacking. to determine the prevalence of selected vector-borne pathogens in dogs from haiti, we tested blood samples collected from 210 owned dogs, 28 (13.3%) of which were infested with rhipicephalus sanguineus ticks at the time of blood collection. no other tick species were identified on these dogs. a commercially available elisa identified antibodies to ehrlichia spp. in 69 (32.9%), antibodies to ...201627270383
dermacentor reticulatus: a vector on the rise.dermacentor reticulatus is a hard tick species with extraordinary biological features. it has a high reproduction rate, a rapid developmental cycle, and is also able to overcome years of unfavourable conditions. dermacentor reticulatus can survive under water for several months and is cold-hardy even compared to other tick species. it has a wide host range: over 60 different wild and domesticated hosts are known for the three active developmental stages. its high adaptiveness gives an edge to th ...201627251148
passive surveillance of ixodes scapularis (say), their biting activity, and associated pathogens in massachusetts.a passive surveillance of tick-borne pathogens was conducted over a 7-year period (2006-2012), in which a total of 3551 ticks were submitted to the university of massachusetts for pcr testing. the vast majority of these ticks were ixodes scapularis from massachusetts (n = 2088) and hence were the focus of further analysis. two taqman duplex qpcr assays were developed to test i. scapularis ticks for the presence of three human pathogens: borrelia burgdorferi, anaplasma phagocytophilum, and babesi ...201627248292
molecular detection and phylogenetic analysis of hepatozoon spp. in questing ixodes ricinus ticks and rodents from slovakia and czech republic.by amplification and sequencing of 18s rrna gene fragments, hepatozoon spp. dna was detected in 0.08 % (4/5057) and 0.04 % (1/2473) of questing ixodes ricinus ticks from slovakia and czech republic, respectively. hepatozoon spp. dna was also detected in spleen and/or lungs of 4.45 % (27/606) of rodents from slovakia. prevalence of infection was significantly higher in myodes glareolus (11.45 %) than in apodemus spp. (0.28 %) (p < 0.001). sequencing of 18s rrna hepatozoon spp. gene amplicons from ...201627245074
francisella-like endosymbiont in a tick collected from a chicken in southern thailand.francisella is a genus of bacterial pathogens potentially lethal to humans. we report here for the first time a novel francisella-like endosymbiont discovered in a hard-tick (rhipicephalus sanguineus s.l.) obtained from a chicken (gallus domesticus) in thailand. the phylogenetic results indicate the 16s rdna sequences of this francisella bacterium form a unique clade with the francisella-like endosymbiont of the tick species, amblyomma varanense and amblyomma helvolum, that have previously been ...201627244963
efficacy of fluralaner spot-on solution against induced infestations with rhipicephalus sanguineus on dogs.the efficacy of fluralaner spot-on solution administered once topically against induced infestations with rhipicephalus sanguineus was evaluated in dogs over a 12-week post-treatment period.201627241176
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