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preliminary investigation on tick fauna in the neighborhood of tarquinia, lazio, italy.this study represents a preliminary inquiry on tick fauna composition carried out in maremma, central italy, where very few data are available.201525857387
tick species (acari: ixodida) in antalya city, turkey: species diversity and seasonal activity.ticks (acari: ixodida) are an important group of ectoparasites of vertebrates. most species are known vectors of diseases including lyme disease, q fever, and crimean-congo hemorrhagic fever. a 3-year research was conducted in antalya, turkey, to determine tick species composition, seasonal abundance, and spatial distribution. the study was carried out in five districts (aksu, dosemealtı, kepez, konyaaltı, and muratpasa) of antalya metropolitan municipality area in turkey, between may 2010 and m ...201525869959
isolation of rickettsia massiliae from rhipicephalus sanguineus ticks, buenos aires (argentina).rickettsia massiliae , a member of the spotted fever group of rickettsia, was first isolated from a rhipicephalus turanicus tick in france. in the new world, it has been detected in rhipicephalus sanguineus ticks from different geographical locations in argentina and the united states, but it has only been isolated in arizona. the aim of this study was the isolation and genetic characterization of r. massiliae from r. sanguineus ticks collected from dogs in buenos aires city, argentina. in total ...201526301512
altitudinal and seasonal differences of tick communities in dogs from pastoralist tribes of northern kenya.studies regarding the distribution and ecology of ticks in dogs from eastern africa are scarce. our research was based on a long-term screening of ticks parasitising the domestic dogs living with indigenous people around lake turkana, mt. kulal and mt. nyiru areas, northern kenya. a total of 9977 ticks were collected from 1464 dogs of all ages and both sexes. identification was performed using morphological keys and data were analyzed using the repeated measures anova, post-hoc scheffe test and ...201526343303
an update on the ecological distribution of the ixodidae ticks in zimbabwe.in total 7657 ticks were collected from 121 dip tanks in 12 districts representative of zimbabwe's five ecological regions between september 2013 and may 2014. based on morphological traits four genera and 13 species of ticks were identified. amblyomma hebraeum (60.3 %), rhipicephalus microplus (58.7 %), rhipicephalus decoloratus (47.1 %), rhipicephalus appendiculatus (56.2 %), rhipicephalus evertsi evertsi (67.8 %), rhipicephalus (near) punctatus (13.2 %), hyalomma truncatum (38 %) and hyalomma ...201525721256
molecular detection of rickettsia species within ticks (acari: ixodidae) collected from arkansas united states.rocky mountain spotted fever (rmsf), caused by the etiological agent rickettsia rickettsii, is the most severe and frequently reported rickettsial illness in the united states, and is commonly diagnosed throughout the southeast. with the discoveries of rickettsia parkeri and other spotted fever group rickettsiae (sfgr) in ticks, it remains inconclusive if the cases reported as rmsf are truly caused by r. rickettsii or other sfgr. arkansas reports one of the highest incidence rates of rmsf in the ...201526334827
rickettsia parkeri infecting free-living amblyomma triste ticks in the brazilian pantanal.the present study evaluated the infection of rickettsiae in 151 rhipicephalus sanguineus, 59 amblyomma ovale, 166 amblyomma triste, one amblyomma dissimile and four amblyomma dubitatum ticks collected in the municipality of poconé, state of mato grosso, within the pantanal biome of brazil. ticks were individually processed by the hemolymph test with gimenez staining, isolation of rickettsia in vero cell culture by the shell vial technique, and polymerase chain reaction (pcr) targeting the citrat ...201525650348
potential of the chemical dinotefuran in the control of rhipicephalus sanguineus (latreille, 1806) (acari: ixodidae) semi-engorged female ticks.ticks are vectors of several pathogens to vertebrates, including the human being. they produce lesions on the hosts during the blood feeding and great economic losses. several chemical acaricides have been used in an attempt to control tick infestations; however these substances are harmful to both the human being and non-target organisms, and to the environment. therefore, there is a need to fight these ectoparasites through less harmful methods, less aggressive to the environment, non-target o ...201525956944
morphological effects of neem (azadirachta indica a. juss) seed oil with known azadirachtin concentrations on the oocytes of semi-engorged rhipicephalus sanguineus ticks (acari: ixodidae).the concern about the harmful effects caused by synthetic pesticides has led to the search for safe and ecological alternatives for pest control. in this context, the neem tree (azadirachta indica) stands out due to its repellent properties and effects on various arthropods, including ticks. for this reason, this study aimed to demonstrate the potential of neem as a control method for rhipicephalus sanguineus ticks, important vectors of diseases in the veterinary point of view. for this, r. sang ...201525346198
comparative efficacy of two oral treatments for dogs containing either afoxolaner or fluralaner against rhipicephalus sanguineus sensu lato and dermacentor reticulatus.the present study compares the efficacy of two recent oral ectoparasiticides containing isoxazolines (nexgard(®), containing afoxolaner and administered at a monthly regimen, and bravecto™ containing fluralaner and administered at a tri-monthly regimen) against rhipicephalus sanguineus sensu lato and dermacentor reticulatus ticks on dogs. 24 dogs were randomly allocated to untreated control, nexgard(®) treated, and bravecto™ treated groups. the treatments were administered on days 0, 28 and 56 f ...201525716658
urban stray cats infested by ectoparasites with zoonotic potential in greece.a large population of stray cats is encountered in many urban areas sharing the same environment with people, usually being in a close direct contact with them. a variety of ectoparasites can infest such cats, causing mild dermatological abnormalities to more severe systemic disorders. in order to determine the extent of which stray cats carry ectoparasites, particularly those of zoonotic potential, 341 stray cats originating from the urban area of thessaloniki, greece, were examined between 201 ...201526319525
ticks infesting wild and domestic animals and humans of sri lanka with new host records.an island-wide collection of tick species infesting humans, domesticated and wild animals and questing ticks in domestic and peridomestic environments was carried out during 2009-2011. a total of 30,461 ticks were collected from 30 different hosts and free living stages from the ground. the collection consisted of 22 tick species from 30 different hosts recording 12 tick species from humans, 19 from domesticated animals and 21 from wild animals, with a total of 97 new host records. the most comm ...201525445744
[epidemiological investigation of predominance tick and the infectious status of severe fever thrombocytopenia syndrome virus in penglai and laizhou counties, shandong province].to investigate the predominance ticks and the infectious status of severe fever with thrombocytopenia (sftsv) in penglai and laizhou counties, shandong province.201526833011
molecular screening for rickettsia, anaplasmataceae and coxiella burnetii in rhipicephalus sanguineus ticks from malaysia.a total of 44 rhipicephalus sanguineus ticks collected from 23 dogs from malaysia were screened for rickettsia, anaplasmataceae and coxiella burnetii. coxiella burnetii was detected in 59% (26/44) of ticks however rickettsia and anaplasmataceae were not detected in any of the ticks. in order to genotype the strains of c. burnetii, multispacer sequence typing (mst) was carried out using three different spacers. one of the spacers; cox2 successfully amplified a fragment for which the full length s ...201526691268
rickettsial diseases and ectoparasites from military bases in japan.ectoparasitic arthropods are often vectors of rickettsiosis. we conducted a survey of ectoparasites on u.s. military facilities throughout japan with the use of specimens submitted by pest control, public health, and veterinary personnel. over 1,600 individual ectoparasites were collected. fifteen species were identified, including several significant vectors of human diseases such as scrub typhus and rickettsial spotted fevers. these ectoparasites included ctenocephalides felis , haemaphysalis ...201525548900
multiplex pcr for molecular screening of borrelia burgdorferi sensu lato, anaplasma spp. and babesia spp.ticks transmit a great variety of pathogenic microorganisms to humans and animals. the detection of tick-borne pathogens (tbp) is mainly by molecular techniques based on polymerase chain reactions (pcr).201526706969
seroprevalence survey of babesia gibsoni infection and tick species in dogs in east china.a seroprevalence survey of babesia gibsoni infection in dogs in east china was conducted using an elisa with recombinant b. gibsoni thrombospondin-related adhesive protein (bgtrap). a total of 1170 dogs from east china were examined and the seroprevalence was 9.23%. the proportion of samples was 81.2%, 7.86% and 10.94% from pet, working and fighting dogs, respectively. the fighting dogs showed highest seroprevalence (39.8%) compared with working dogs (26.1%) and pet dogs (3.47%). these results i ...201526483164
infection with ehrlichia canis and anaplasma platys (rickettsiales: anaplasmataceae) in two lineages of rhipicephalus sanguineus sensu lato (acari: ixodidae) from argentina.natural infection with ehrlichia canis and anaplasma platys in ticks belonging to the tropical and temperate lineages of rhipicephalus sanguineus sensu lato from argentina was evaluated. samples were tested for ehrlichia canis infection by pcr assays using 16s rrna, dsb and p28 gene, while detection of a. platys was performed with 16s rrna and groesl gene. the assignment of the ticks to each lineage was corroborated with 16s rdna sequences. all ticks infected with e. canis and a. platys belonged ...201526100492
molecular detection of anaplasma platys and ehrlichia canis in dogs from kabylie, algeria.ehrlichia canis and anaplasma platys are bacteria belonging to the anaplasmataceae family that cause acute, self-limiting and sometimes fatal vector-borne infections in dogs. these bacteria have been reported worldwide and are transmitted mainly by rhipicephalus sanguineus. aside from a report on e. canis once in 1935, no other anaplasmataceae bacteria have been reported in algeria to date. the aim of this study was to identify the microbial species implicated in ehrlichiosis and anaplasmosis by ...201525583345
canine vector-borne co-infections: ehrlichia canis and hepatozoon canis in the same host monocytes.the protozoon hepatozoon canis and the rickettsia ehrlichia canis are tick-borne pathogens, transmitted by rhipicephalus sanguineus, which cause canine hepatozoonosis and canine monocytic ehrlichiosis, respectively. co-infection of the same host monocytes with h. canis and e. canis confirmed by molecular characterization of the infecting agents and quantitative assessment of co-infected cells is described for the first time in three naturally-infected dogs. blood smear evaluation indicated that ...201525560923
comparative profiling of micrornas in male and female rhipicephalus sanguineus.rhipicephalus sanguineus is an ectoparasite of medical and veterinary significance, which can transmit a number of pathogens including babesia canis, ehrlichia canis, and rickettsia conorii. micrornas (mirnas) are recognized as regulators in sex differentiation in dioecious species. we here investigated and compared the mirna profiles of male and female adults of r. sanguineus by combining solexa deep sequencing with bioinformatics platform and quantitative real-time pcr. a total of 11.88 and 16 ...201526054615
molecular identification of theileria and babesia in ticks collected from sheep and goats in the black sea region of turkey.a molecular survey was undertaken in the black sea region of turkey to determine the presence of theileria and babesia species of medical and veterinary importance. the ticks were removed from sheep and goats, pooled according to species and locations, and analyzed by pcr-based reverse line blot (rlb) and sequencing. a total of 2241 ixodid ticks belonging to 5 genus and 12 species were collected and divided into 310 pools. infection rates were calculated as the maximum likelihood estimation (mle ...201525260692
tick-borne diseases (borreliosis, anaplasmosis, babesiosis) in german and austrian dogs: status quo and review of distribution, transmission, clinical findings, diagnostics and prophylaxis.tick-borne diseases (tbd) in dogs have gained in significance in german and austrian veterinary practices. the widespread european tick species ixodes ricinus represents an important vector for spirochaetes of the borrelia burgdorferi sensu lato group and rickettsiales such as anaplasma phagocytophilum. the meadow or ornate dog tick (dermacentor reticulatus) is an important vector for babesia canis, as is the brown dog tick (rhipicephalus sanguineus) for babesia vogeli in the mediterranean regio ...201526152408
spatial distribution of anaplasma phagocytophilum and hepatozoon canis in red foxes (vulpes vulpes) in hungary.in recent years, ehrlichia canis and hepatozoon canis transmitted by rhipicephalus sanguineus were reported from hungary. the aim of the present study was to reveal the spatial distribution pattern of pathogens transmitted by r. sanguineus in a sentinel species, red fox (vulpes vulpes) in hungary and to analyse the relationship of these patterns with landscape and climate by geographical information systems. fox carcasses, representing 0.5% of the total fox population were randomly selected out ...201526065623
crimean-congo hemorrhagic fever virus clade iv (asia 1) in ticks of western iran.crimean-congo hemorrhagic fever virus (cchfv) is transmitted through the bite of an infected tick, or by direct contact with cchfv-infected patients' blood or the products of infected livestock. in 2012, ticks were collected in eight regions of lorestan province, iran. in total, 434 ticks were collected. reverse transcriptase polymerase chain reaction was used for the detection of cchfv rna. of 434 ticks, 419 (96.6%) ticks were from the family ixodidae (hard ticks) and 15 (3.5%) ticks were from ...201526336221
rhipicephalus rossicus, a neglected tick at the margin of europe: a review of its distribution, ecology and medical importance.rhipicephalus rossicus (ixodida: ixodidae) is a three-host tick with a broad host spectrum that includes wild animals, pets, livestock and humans. despite its local abundance in certain areas, most of the available information on r. rossicus was published decades ago, mainly by former soviet authors. its distribution largely overlaps the eurasian steppe. however, its range may be more extensive than is currently known because this species may have been misidentified as rhipicephalus sanguineus, ...201525761411
[factors associated with ehrlichia canis infection in dogs infested with ticks from huanuco, peru].the aim of the study was to determine the frequency and associated factors of ehrlichia canis infection in dogs. blood samples from 150 dogs infested with ticks in 10 veterinary clinics in the city of huanuco in peru were collected. the dogs were randomly selected without regard to breed, age or sex. ehrlichia canis antibodies were detected by chromatographic immunoassay.51.3% of dogs were infected with ehrlichia canis. in the multivariate analysis, factors associated with the presence of ehrlic ...201526732926
molecular diagnosis of ehrlichia canis in dogs and ticks rhipicephalus sanguineus (acari: ixodidae) in yucatan, mexico.ehrlichia canis is the etiological agent behind canine monocytic ehrlichiosis, and the tick rhipicephalus sanguineus (latreille) is its main vector. blood smear and nested polymerase chain reaction (pcr) techniques were used to identify e. canis infection in dogs and r. sanguineus, and explore factors possibly associated with infection in dogs in yucatan, mexico. blood samples were taken and ticks r. sanguineus collected from 50 dogs (10 house dogs and 40 in an animal control center). data were ...201526336286
seroprevalence of tick-borne pathogens and tick infestation in dogs from tapirapé and karajá indigenous communities, brazil.with the aim of studying some tick-borne diseases, a total of 327 dogs (114 from tapirapé and 213 from karajá indigenous ethnicity, mato grosso, mt, brazil) were sampled. serum samples were submitted to the indirect fluorescence antibody test (ifat) to detect antibodies against babesia vogeli, ehrlichia canis, and rickettsia spp. possible associations of risk factors and the occurrence of seroreativity to tick-borne agents and tick infestations were analyzed through chi-squared tests. among 327 ...201526186512
the efficacy of a generic doxycycline tablet in the treatment of canine monocytic ehrlichiosis.the objective of the present study was to evaluate the therapeutic efficacy of a generic doxycycline tablet (doxyvet) against ehrlichia canis infection in dogs. canine monocytic ehrlichiosis is caused by the bacterium e. canis and transmitted by the brown kennel tick (rhipicephalus sanguineus). six disease-free and tick-free dogs were infested with e. canis infected ticks. once diagnosed (with polymerase chain reaction [pcr] analysis and platelet counts) as positive for infection, doxycycline ta ...201526018824
survey of ehrlichia canis, babesia spp. and hepatozoon spp. in dogs from a semiarid region of brazil.this study assessed the occurrence of ehrlichia spp., babesia spp. and hepatozoon spp. infections in 100 tick-harboring dogs from a semiarid region of the state of paraíba, northeastern brazil. blood samples and ticks were collected from the animals, and a questionnaire was submitted to dog owners to obtain general data. blood samples were used to perform hemogram, direct blood smear and immunological and molecular hemoparasite detection. the 1,151 ticks collected were identified as rhipicephalu ...201525909253
a serological and molecular survey of babesia vogeli, ehrlichia canis and rickettsia spp. among dogs in the state of maranhão, northeastern brazil.this study evaluated exposure and infection by tick-borne agents (babesia vogeli, ehrlichia canis and rickettsia spp.) in 172 dogs in rural areas and 150 dogs in urban areas of the municipality of chapadinha, state of maranhão, northeastern brazil, using molecular and serological methods. overall, 16.1% of the sampled dogs (52/322) were seroreactive to b. vogeli, with endpoint titers ranging from 40 to 640. for e. canis, 14.6% of the dogs (47/322) were seroreactive, with endpoint titers from 80 ...201525909250
further thoughts on the taxonomy and vector role of rhipicephalus sanguineus group ticks.rhipicephalus sanguineus is a tick species described in 1806 by latreille, based on specimens probably collected in france. however, this is a taxon with uncertain morphological definition and recent studies have gathered irrefutable evidence supporting the existence of a cryptic species-complex under the name 'r. sanguineus', whose number of sibling species around the world has yet to be ascertained. this fact is of great medical and veterinary concern, also considering that ticks currently ide ...201525579394
rickettsia rickettsii and rickettsia felis infection in rhipicephalus sanguineus ticks and ctenocephalides felis fleas co-existing in a small city in yucatan, mexico. 201526611982
levels of certain endothelial biomarkers during the acute phase and convalescence in patients with different severity of mediterranean spotted fever.mediterranean spotted fever (msf) is a tick-borne disease caused by rickettsia conorii subspp. conorii. it is transmitted by the bite of the tick rhipicephalus sanguineus. modified by the rickettsial invasion, the micro-vascular endothelium acquires an activated inflammatory phenotype and initiates secretion of cytokines and expression of cell adhesion molecules (cams) and chemoattractans.201524712278
the role of rhipicephalus sanguineus sensu lato saliva in the dissemination of rickettsia conorii in c3h/hej mice.animal models have been developed for the study of rickettsial pathogenesis. however, to understand what occurs during the natural route of rickettsial transmission via the tick bite, the role of tick saliva should be considered in these models. to address this, we analysed the role of tick saliva in the transmission of rickettsia conorii (rickettsiales: rickettsiaceae) in a murine host by intradermally (i.d.) inoculating two groups of susceptible c3h/hej mice with this rickettsia, and infesting ...201526011701
mediterranean spotted fever and hearing impairment: a rare complication.mediterranean spotted fever (msf) is caused by rickettsia conorii and transmitted by the brown dog tick rhipicephalus sanguineus. it is prevalent in southern europe, africa and central asia. the disease usually has a benign course and is characterized by fever, myalgia and a characteristic papular rash with an inoculation eschar ('tache noir') at the site of the tick bite. severe forms of disease can have cardiac, neurologic or renal involvement. nervous system complications are unusual and may ...201525892247
identification of tick species and disseminate pathogen using hemolymph by maldi-tof ms.matrix assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) is increasingly emerging tool for identification of arthropods including tick vectors using whole or body part of specimens. the challenges of the present study were to assess maldi-tof ms profiling for the both identification of tick species and rickettsia spp. in infected ticks using hemolymph as protein mixture.201526051210
hemocytes of rhipicephalus sanguineus (acari: ixodidae): characterization, population abundance, and ultrastructural changes following challenge with leishmania infantum.few studies have examined the cellular immune response of ticks, and further research on the characterization of the hemocytes of ticks is required, particularly on those of rhipicephalus sanguineus (latreille) because of the medical and veterinary importance of this tick. the aims of this study were to characterize the morphology and the ultrastructure of the different types of hemocytes of adult r. sanguineus and to determine the population abundance and the ultrastructural changes in the hemo ...201526336264
molecular detection of crimean-congo haemorrhagic fever virus in ticks collected from infested livestock populations in a new endemic area, south of iran.crimean-congo haemorrhagic fever (cchf) is a viral zoonotic disease with potentially fatal systemic effects on man. we aimed to determine the presence of cchf virus among collected ticks from domestic livestock from october 2012 to september 2013.201626758985
molecular detection of theileria, babesia, and hepatozoon spp. in ixodid ticks from palestine.ixodid ticks transmit various infectious agents that cause disease in humans and livestock worldwide. a cross-sectional survey on the presence of protozoan pathogens in ticks was carried out to assess the impact of tick-borne protozoa on domestic animals in palestine. ticks were collected from herds with sheep, goats and dogs in different geographic districts and their species were determined using morphological keys. the presence of piroplasms and hepatozoon spp. was determined by pcr amplifica ...201626969489
determination the role of rhipicephalus sanguineus for transmission of leishmania major to reservoir animals.this study aimed to explore the role of rhipicephalus sanguineus in the transmission of leishmania major, the etiological agent of zoonotic cutaneous leishmaniasis.201628091384
molecular detection and characterization of spotted fever group rickettsiae in ticks from central italy.the aim of this study was to investigate the presence of rickettsial pathogens in ticks from central italy. a total of 113 ticks hailed from latium and tuscany regions were identified and tested by pcr to detect glta, ompa, ompb genes of rickettsia. positive amplicons were sequenced and identified at species level. ticks were analyzed individually or in pools. the percentage of positivity for sfg rickettsiae was 12.4%, expressed as minimum infection rate (mir) assuming that one tick was positive ...201627365155
occurrence of mycoplasma haemocanis in dogs infested by ticks in campo grande, mato grosso do sul, brazil.hemotropic mycoplasmas in dogs, such as mycoplasma haemocanis, have been described worldwide. recently, these pathogens have been reported to be causative agent of zoonosis. it is known that its transmission may occur through the action of blood-sucking arthropods (e.g. ticks or fleas), through blood transfusion, contaminated fomites and/or transplacentally. in brazil, m. haemocanis is present in practically all regions and the tick rhipicephalus sanguineus sensu lato is suspected the main vecto ...201627580391
effect of heat stress and oil formulation on conidial germination of metarhizium anisopliae s.s. on tick cuticle and artificial medium.the effect of heat stress (45°c) versus non-heat stress (27°c) on germination of metarhizium anisopliae sensu stricto (s.s.) isolate ip 119 was examined with conidia formulated (suspended) in pure mineral oil or in water (tween 80, 0.01%), and then applied onto the cuticle of rhipicephalus sanguineus sensu lato (s.l.) engorged females or onto culture medium (pday). in addition, bioassays were performed to investigate the effect of conidia heated while formulated in oil, then applied to blood-eng ...201627317831
efficacy of water- and oil-in-water-formulated metarhizium anisopliae in rhipicephalus sanguineus eggs and eclosing larvae.conidia of the entomopathogenic fungus metarhizium anisopliae (ascomycota: clavicipitaceae) were assessed against rhipicephalus sanguineus (arachnida: ixodidae) eggs under laboratory conditions. clusters of 25 eggs were applied either directly with the fungal conidial formulations or set on previously fungus-treated filter paper. treatments consisted of conidia formulated in water or an oil-in-water emulsion at final concentrations of 3.3 × 10(3), 10(4), 3.3 × 10(4), 10(5), or 3.3 × 10(5) conidi ...201626364059
detection of rickettsiae in fleas and ticks from areas of costa rica with history of spotted fever group rickettsioses.outbreaks of spotted fevers have been reported in costa rica since the 1950s, although vectors responsible for transmission to humans have not been directly identified. in this study, species of rickettsia were detected in ectoparasites from costa rica, mostly from five study sites where cases of spotted fevers have been reported. ticks and fleas were collected using drag cloths or directly from domestic and wild animals and pooled according to species, host, and location. pools were analyzed in ...201627592065
rickettsia (rickettsiales: rickettsiaceae) vector biodiversity in high altitude atlantic forest fragments within a semiarid climate: a new endemic area of spotted-fever in brazil.rickettsioses are re-emerging vector-borne zoonoses with a global distribution. recently, rickettsia sp. strain atlantic rainforest has been associated with new human spotted-fever (sf) cases in brazil, featuring particular clinical signs: eschar formation and lymphadenopathy. these cases have been associated with the tick species, amblyomma ovale from 2010 until 2015, the brazilian health department confirmed 11 human sf cases in the maciço de baturité region, ceará, brazil. the present study r ...201627480099
fleas and ticks in carnivores from a domestic-wildlife interface: implications for public health and wildlife.fleas and ticks are parasites of wild and domestic mammals, and can be vectors of several pathogens. in rural areas, domestic carnivores such as the domestic dog (canis lupus familiaris l.), may act as a "bridge" between natural areas and human settlements where ectoparasites can be used as a metric of such link. the aim of this study was to identify fleas, ticks, and rickettsia spp., collected from domestic and wild carnivores in a natural reserve and surrounding human settlements in central ch ...201627539151
exposure of dogs to spotted fever group rickettsiae in urban sites associated with human rickettsioses in costa rica.the zoonotic transmission cycles of rickettsia rickettsii and other spotted fever group (sfg) rickettsiae in latin america have usually been associated with rural or sylvatic environments, although domestic dogs can be implicated in more populated settings. in this study, exposure of dogs to sfg rickettsiae in the greater metropolitan area of costa rica was investigated. dogs from sites associated with human cases and from dog shelters were evaluated by indirect immunofluorescence assay (ifa) us ...201626995323
a novel ehrlichial agent detected in tick in french polynesia.ticks are hematophageous arthropods that are known to host and transmit miscellaneous pathogens including zoonotic bacteria. the aim of this study was to investigate the presence of tick-associated microorganisms in tahiti, french polynesia with molecular tools. a total of 658 ticks from two species including rhipicephalus sanguineus s.l. and rh. annulatus were collected with forceps on dogs and cattle respectively, or with a flag on pasture in several locations of tahiti in 2013. two rickettsia ...201627522945
coxiella symbionts are widespread into hard ticks.ticks are blood-feeding arthropods and can harbor several bacteria, including the worldwide zoonotic disease q-fever agent coxiella burnetii. recent studies have reported a distinct group of coxiella mostly associated with ixodidae ticks, including the primary endosymbionts of amblyomma americanum. in the present work, a screening for coxiella infection was performed by 16s ribosomal dna (rdna) gene analyses in 293 tick samples of 15 different species sampled worldwide, including brazil, colombi ...201627595990
crimean-congo hemorrhagic fever virus lineages europe 1 and europe 2 in bulgarian ticks.crimean-congo hemorrhagic fever (cchf) is a tick-borne human viral disease with fatality rate up to 30%. two genetic lineages of cchf virus (cchfv) are present in europe - lineage europe 1 which contains pathogenic cchfv strains, and lineage europe 2 consisting of ap92 and ap92-like strains. in order to investigate cchfv distribution and potential risk for humans, ticks were collected from livestock in the five districts where cchf cases have been reported in the last five years in bulgaria. cch ...201627378409
rhipicephalus turanicus, a new vector of hepatozoon canis.the distribution of hepatozoon canis mainly encompasses areas where its main tick vector, rhipicephalus sanguineus sensu lato, is present. however, the detection of this pathogen in dogs, foxes and golden jackals well outside the areas inhabited by this tick species reinforced the hypothesis that additional ixodids are involved in the life cycle and transmission of this protozoon. the present study provides, for the first time, data supporting the sporogonic development of h. canis in specimens ...201627998321
autochthonous hepatozoon infection in hunting dogs and foxes from the czech republic.blood samples from 21 red foxes (vulpes vulpes) and 8 hunting dogs from the same locality in the czech republic were examined for presence of hepatozoon canis/hepatozoon sp. the dogs were selected based on their close contact with foxes during fox bolting and because they had not traveled into known endemic areas. using diagnostic pcr amplifying partial 18s rdna fragment, hepatozoon dna was detected in 20 red foxes (95 %) and 4 dogs (50 %). from 8 positive foxes and 2 positive dogs, we obtained ...201627431805
molecular detection and phylogenetic analysis of hepatozoon spp. in questing ixodes ricinus ticks and rodents from slovakia and czech republic.by amplification and sequencing of 18s rrna gene fragments, hepatozoon spp. dna was detected in 0.08 % (4/5057) and 0.04 % (1/2473) of questing ixodes ricinus ticks from slovakia and czech republic, respectively. hepatozoon spp. dna was also detected in spleen and/or lungs of 4.45 % (27/606) of rodents from slovakia. prevalence of infection was significantly higher in myodes glareolus (11.45 %) than in apodemus spp. (0.28 %) (p < 0.001). sequencing of 18s rrna hepatozoon spp. gene amplicons from ...201627245074
natural transovarial and transstadial transmission of leishmania infantum by naïve rhipicephalus sanguineus ticks blood feeding on an endemically infected dog in shiraz, south of iran.the visceral leishmaniasis parasite, leishmania infantum, is naturally transmitted through the bites of phlebotomine sand flies. alternative routes of transmission are questioned. the main aim is to verify the passage of l. infantum kdna in ticks, rhipicephalus sanguineus, blood feeding on a parasitemic dog in shiraz, south of iran.201627496515
molecular detection and identification of rickettsiales pathogens in dog ticks from costa rica.although vector-borne diseases are globally widespread with considerable impact on animal production and on public health, few reports document their presence in central america. this study focuses on the detection and molecular identification of species belonging to selected bacterial genera (ehrlichia, anaplasma and rickettsia) in ticks sampled from dogs in costa rica by targeting several genes: 16s rrna/dsb genes for ehrlichia; 16s rrna/groel genes for anaplasma, and ompa/glta/groel genes for ...201627480409
prevalence of vector-borne pathogens in dogs from haiti.canine vector-borne pathogens are common on some caribbean islands, but survey data in haiti are lacking. to determine the prevalence of selected vector-borne pathogens in dogs from haiti, we tested blood samples collected from 210 owned dogs, 28 (13.3%) of which were infested with rhipicephalus sanguineus ticks at the time of blood collection. no other tick species were identified on these dogs. a commercially available elisa identified antibodies to ehrlichia spp. in 69 (32.9%), antibodies to ...201627270383
molecular detection of anaplasma platys, ehrlichia canis, hepatozoon canis and rickettsia monacensis in dogs from maio island of cape verde archipelago.tick-borne diseases are emerging worldwide and have an important zoonotic relevance. dogs play an important role in the epidemiology of several zoonotic tick-borne pathogens acting as sentinels and/or reservoirs. this study focused on the molecular identification of tick-borne pathogens in blood samples of 153 autochthonous asymptomatic dogs in maio island, cape verde archipelago. eighty-four (54.9%) dogs were positive for one or more pathogens. fifty-five (35.9%) dogs were infected with hepatoz ...201627177475
serological and molecular investigation of ehrlichia spp. and anaplasma spp. in ticks and blood of dogs, in the thrace region of turkey.in recent years, tick-borne diseases like ehrlichiosis and anaplasmosis became widespread worldwide threatening the health of both human and companion animals. therefore, the aim of this study was to determine the presence of anaplasma spp., and ehrlichia spp. in dogs and ticks in the thrace region of turkey. a total of 400 blood samples and 912 ticks were collected from dogs living in shelters that are located in four cities (istanbul, edirne, tekirdag and kirklareli) of the thrace region. bloo ...201626961274
molecular detection of emerging tick-borne pathogens in vojvodina, serbia.ticks play an important role in disease transmission globally due to their capability to serve as vectors for human and animal pathogens. the republic of serbia is an endemic area for a large number of tick-borne diseases. however, current knowledge on these diseases in serbia is limited. the aim of this study was to investigate the presence of new emerging tick-borne pathogens in ticks collected from dogs and the vegetation from different parts of vojvodina, serbia. a total of 187 ticks, includ ...201626565929
vector-borne diseases in stray dogs in peninsular malaysia and molecular detection of anaplasma and ehrlichia spp. from rhipicephalus sanguineus (acari: ixodidae) ticks.little data are available on the prevalence and transmission of vector-borne diseases in stray dogs in peninsular malaysia. this study was designed to determine the occurrence of vector-borne pathogens in malaysian stray dogs using serological and molecular approaches. in total, 48 dog blood samples were subjected to serological analysis using snap 4dx kit (idexx laboratories, westbrook, me). the presence of ehrlichia and anaplasma dna in the dog blood samples and rhipicephalus sanguineus (latre ...201626494821
molecular characterization of ehrlichia canis infecting dogs, buenos aires.canine monocytic ehrlichiosis (cme) is a worldwide potentially fatal tick-borne rickettsial disease of dogs caused by ehrlichia canis and transmitted by rhipicephalus sanguineus sensu lato. cme diagnosis includes indirect (serology) and direct (e.g. blood smears and pcr) methods. pcr is more sensitive and specific than direct microscopic examination and positive pcr results confirm infection, whereas positive serologic test results only confirm exposure. the aim of the present study was to perfo ...201627236582
molecular detection and characterization of anaplasma platys in dogs and ticks in cuba.canine cyclic thrombocytopenia, an infectious disease caused by anaplasma platys is a worldwide dog health problem. this study aimed to detect and characterize a. platys deoxyribonucleic acid (dna) in dogs and ticks from cuba using molecular methods. the study was conducted in four cities of cuba (habana del este, boyeros, cotorro and san josé de las lajas). blood samples were collected from 100 dogs in these cities. the animals were inspected for the detection of tick infestation and specimens ...201627132516
molecular identification and characterization of anaplasma platys and ehrlichia canis in dogs in mexico.the tick-borne pathogens ehrlichia canis and anaplasma platys are the causative agents of canine monocytic ehrlichiosis (cme) and canine cyclic thrombocytopenia (cct). although molecular evidence of e. canis has been shown, phylogenetic analysis of this pathogen has not been performed and a. platys has not been identified in mexico, where the tick vector rhipicephalus sanguineus sensu lato (s.l.) is common. the aim of this research was to screen, identify and characterize e. canis and a. platys ...201626615872
molecular detection of rickettsia conorii and other zoonotic spotted fever group rickettsiae in ticks, romania.the diverse tick fauna as well as the abundance of tick populations in romania represent potential risks for both human and animal health. spotted fever group (sfg) rickettsiae are recognized as important agents of emerging human tick-borne diseases worldwide. however, the epidemiology of rickettsial diseases has been poorly investigated in romania. in urban habitats, companion animals which are frequently exposed to tick infestation, play a role in maintenance of tick populations and as reservo ...201626507182
a survey of tick-borne pathogens in dogs and their ticks in the pantanal biome, brazil.tick and blood samples collected from domestic dogs in the brazilian pantanal were tested by molecular methods for the presence of tick-borne protozoa and bacteria. among 320 sampled dogs, 3.13% were infected by babesia vogeli (piroplasmida: babesiidae), 8.75% by hepatozoon canis (eucoccidiorida: hepatozoidae), 7.19% by anaplasma platys (rickettsiales: anaplasmataceae), and 0.94% by an unclassified anaplasma sp. in three tick species collected from dogs, the following tick-borne agents were dete ...201626467462
molecular evidence of spotted fever group rickettsiae and anaplasmataceae from ticks and stray dogs in bangladesh.emerging tick-borne diseases (tbds) are important foci for human and animal health worldwide. however, these diseases are sometimes over looked, especially in countries with limited resources to perform molecular-based surveys. the aim of this study was to detect and characterize spotted fever group (sfg) rickettsiae and anaplasmataceae in bangladesh, which are important tick-borne pathogens for humans and animals worldwide. a total of 50 canine blood samples, 15 ticks collected from dogs, and 1 ...201626573516
anaplasma marginale and theileria annulata in questing ticks from portugal.ticks are ubiquitous arthropods and vectors of several pathogenic agents in animals and humans. monitoring questing ticks is of great importance to ascertain the occurrence of pathogens and the potential vector species, offering an insight into the risk of disease transmission in a given area. in this study 428 host-seeking ticks, belonging to nine species of ixodidae and collected from 17 of the 23 portuguese mainland subregions, were screened for several tick-borne agents with veterinary relev ...201627394441
confirmation of occurrence of <i>babesia vogeli</i> in a dog in windhoek, central namibia.although there is evidence of high seroprevalence of antibodies to babesia spp. in dogs in central namibia, clinical babesiosis is rarely diagnosed. rhipicephalus sanguineus sensu lato, the vector of babesia vogeli, is common in namibia while haemaphysalis elliptica, the vector of the highly virulent but morphologically indistinguishable babesia rossi, has rarely been recorded, mainly in northern namibia. on the basis of vector occurrence, clinical cases of canine babesiosis in windhoek, central ...201627796110
genetic diversity and molecular characterization of babesia motasi-like in small ruminants and ixodid ticks from china.ovine babesioses, an important tick-borne disease of sheep and goats in china, is caused by the reproduction of intraerythrocytic protozoa of the babesia genus. babesia motasi-like is a babesia parasite that infects small ruminant in china, and two sub-groups of b. motasi-like can be subdivided based on differences in the rhoptry-associated-protein-1 gene. this study aimed to characterize the distribution, epidemiology and genetics of b. motasi-like in animals and ticks. a molecular investigatio ...201626976477
molecular epidemiological surveillance to assess emergence and re-emergence of tick-borne infections in tick samples from china evaluated by nested pcrs.an investigation was performed to detect eight pathogens in ticks collected from grass tips or animals in the southern, central and northeast regions of china. dna samples extracted from ticks were collected from ten different locations in eight provinces of china and subjected to screening for tick-borne pathogens, including borrelia burgdorferi sensu lato, ehrlichia spp., rickettsia spp., babesia/theileria spp., ehrlichia ruminantium, coxiella burnetii, and francisella tularensis, using nested ...201626943995
molecular evidence of vector-borne pathogens in dogs and cats and their ectoparasites in algiers, algeria.in algeria, only limited information is currently available on the prevalence of emergent canine and feline vector-borne diseases. the aim of the present work was to detect by qpcr vector-associated bacteria in stray dogs and cats and their ectoparasites from algiers. 18/117 (15.38%) dogs and 2/107 (1.87%) cats were positive for at least one vector-borne agent. coxiella burnetii and bartonella henselae were identified in 1/117 (0.85%) dog individually. ehrlichia canis dna was detected in 17/117 ...201627012917
serosurvey of borrelia in dogs, horses, and humans exposed to ticks in a rural settlement of southern brazil.the aims of the present study were to serosurvey dogs, horses, and humans highly exposed to tick bites for anti-borrelia burgdorferi s.l. antibodies, identify tick species present, and determine risk factors associated with seropositivity in a rural settlement of paraná state, southern brazil. eighty-seven residents were sampled, along with their 83 dogs and 18 horses, and individual questionnaires were administered. immunofluorescence antibody test (ifat) was performed on serum samples and posi ...201627982299
genetic and immunological evidences of borrelia burgdorferi in dog in thailand.lyme disease is a tick-borne zoonotic disease caused by spirochete borrelia burgdorferi. it is transmitted from animals to humans by the bite of infected ticks of the genus ixodes. although lyme disease has been reported in china and japan, the disease has never been reported in thailand. blood samples and ticks were collected from 402 dogs from 7 and 3 animal clinics in chiang mai and phuket provinces, thailand, respectively. blood samples were tested for antibodies against b. burgdorferi, anap ...201627086427
molecular identification of borrelia genus in questing hard ticks from portugal: phylogenetic characterization of two novel relapsing fever-like borrelia sp.in the last decades, several studies have reported pathogenic species of borrelia related to those that cause tick-borne relapsing fever (rf), but unexpectedly suggesting their transmission by hard ticks, known vectors of borrelia burgdorferi sensu lato (b. burgdorferi s.l.) species, rather than by soft ticks. this study was designed to update the presence of b. burgdorferi s.l. species in ticks from several districts of mainland portugal, where ixodes ricinus had been previously described. tick ...201626976475
the diversity and prevalence of hard ticks attacking human hosts in eastern siberia (russian federation) with first description of invasion of non-endemic tick species.hard ticks are the vectors of many pathogens including tick-borne encephalitis virus and the lyme disease agent borrelia burgdorferi sensu lato. in eastern siberia, ixodes persulcatus, dermacentor nuttalli, dermacentor silvarum and haemaphysalis concinna are regarded as aggressive to humans. recently, significant changes in world tick fauna have been reported and this affects the spread of tick-borne pathogens. we studied the current species diversity, population structure and prevalence of tick ...201626443685
antifungal, antiradical and cytotoxic activities of extractives obtained from tagetes patula l. (asteraceae), a potential acaricide plant species.tagetes patula l. shows a complex chemical composition, ranging from glycosylated flavonoids and thiophenes in extracts until terpenoids in the essential oil. in the present study, due to this rich flavonoidic constitution, its antioxidant potential was determined, having shown values of antiradical percentage superior to reference compounds, mainly the extracts prepared with flowers. previous studies performed emphasized the acaricide potential of t. patula and thus, the present study aimed to ...201626945559
first molecular evidence of coxiella burnetii infecting ticks in cuba.coxiella burnetii is the causative agent of q fever. in order to explore the occurrence of c. burnetii in ticks, samples were collected from horses, dogs and humans living in a cuban occidental community. the species most commonly recovered were amblyomma mixtum (67%), rhipicephalus sanguineus s.l. (27%) and dermacentor nitens (6%). specific is1111 pcr and amplicon sequencing allowed the identification of c. burnetii dna in a. mixtum collected from a domestic horse. these findings, for first tim ...201626372520
molecular analysis of rhipicephalus sanguineus (acari: ixodidae), an incriminated vector tick for babesia vogeli in taiwan.the genetic identity of rhipicephalus sanguineus tick was determined for the first time in taiwan. the phylogenetic relationships were analyzed by comparing the sequences of mitochondrial 16s ribosomal dna gene obtained from 32 strains of ticks representing six species of rhipicephalus, two species of dermacentor and two outgroup species (haemaphysalis inermis and ixodes ricinus). seven major clades can be easily distinguished by neighbour-joining analysis and were congruent by maximum-parsimony ...201627815655
molecular, biological, and morphometric comparisons between different geographical populations of rhipicephalus sanguineus sensu lato (acari: ixodidae).in this study, different geographical populations of rhipicephalus sanguineus sensu lato were compared by molecular, biological, and morphometric methods. phylogenetic trees were constructed using 12s and 16s rdna sequences and showed two distinct clades: one composed of ticks from brazil (jaboticabal, sp), cuba (havana) thailand (bangkok) and the so-called "tropical strain" ticks. the second clade was composed of ticks from spain (zaragoza), argentina (rafaela, santa fe) and the so-called "temp ...201626790741
dinotefuran-induced morphophysiological changes in the ovaries and midgut of semi-engorged females rhipicephalus sanguineus latreille, 1806 (acari: ixodidae) ticks.the present study demonstrated the effects of dinotefuran (active compound of the protetor pet® acaricide) in germ cells and the digestive processes of semi-engorged females of r. sanguineus exposed to different concentrations of the chemical. for this purpose, 120 semi-engorged females were divided into four treatment groups with 30 individuals each: group i or control (distilled water), group ii (5000 ppm), group iii (6250 ppm), and group iv (8334 ppm of dinotefuran). all ticks were immersed i ...201626614361
species diversity and spatial distribution of ixodid ticks on small ruminants in greece.a cross sectional field study on the distribution of ixodidae ticks was carried out over two consecutive tick seasons (2012 and 2013) in small ruminants (309 farms) located in mainland and five islands of greece. a total of 2108 ticks (1199 females, 908 males, and 1 nymph) were collected from sheep and goats. two species of rhipicephalus, two of ixodes, five of hyalomma, three of haemaphysalis, and one of dermacentor were detected. the species with the widest distribution were rhipicephalus sang ...201627655133
efficacy of a novel oral formulation of sarolaner (simparica™) against four common tick species infesting dogs in europe.the efficacy of single oral treatment of sarolaner (simparica™, zoetis), a novel isoxazoline compound, was evaluated against four tick species known to commonly infest dogs in europe. eight laboratory studies were conducted using adult purpose-bred beagle dogs. in each study, 16 animals were randomly allocated to one of two treatment groups based on pre-treatment host-suitability tick counts. dogs were infested with 50 unfed adult dermacentor reticulatus (two studies), ixodes hexagonus (three st ...201627068640
evaluation of pharmacokinetics and efficacy of ivermectin following oral administration in dogs against experimental infection of ctenocephalides felis felis and rhipicephalus sanguineus.with the increasing number of pets in home the human-animal relationship is increasingly close and care about control disease growing. ivermectin (ivm) is frequently used because its proven safety. ivm is recommended for the treatment of demodectic scabies and prevention of heartworm in dogs, but informally is extremely used to control of ctenocephalides felis felis and rhipicephalus sanguineus. the aim of this study is evaluate the use of ivm in dogs, by the oral route at 0.6μg/kg dose, against ...201627692320
first detection and molecular identification of babesia vogeli from rhipicephalus sanguineus (acari: ixodidae) in taiwan.a total of 578 rhipicephalus sanguineus ticks collected from dogs in taiwan were examined for babesia by species-specific polymerase chain reaction assay based on the 18s small subunit ribosomal rna (ssrrna) gene. babesia dna was detected in 1.04 % (6/578) of rh. sanguineus ticks. phylogenetic analysis revealed that these babesia spp. were genetically linked to the same clade within the genospecies of babesia vogeli and could be discriminated from other genospecies of babesia. intra-species anal ...201626796569
novel phleboviruses detected in ticks, greece.since 2009, when severe fever with thrombocytopenia syndrome virus and heartland virus have been identified and associated with disease in humans, the interest on tick-borne phleboviruses is increasing rapidly. the aim of the present study was to investigate the presence of tick-borne phleboviruses in greece and compare them with respective ones detected worldwide. ticks collected from goats and sheep in 60 sites of 13 regional units of greece were grouped in pools (1-3 ticks per pool) and teste ...201626935112
prevalence and infection intensity of rickettsia massiliae in rhipicephalus sanguineus sensu lato ticks from mendoza, argentina.rickettsia massiliae belongs to the spotted fever group and in the new world is commonly associated with the brown dog tick, rhipicephalus sanguineus. herein we investigate the presence of r. massiliae in rh. sanguineus sensu lato ticks in a location near the andean foothills (mendoza, argentina), to provide a prevalence estimate and to assess the infection intensity of this pathogen. rickettsia massiliae infection was found in 5.1% of the rh. sanguineus s.l ticks analyzed, all with high infecti ...201627394145
isolation and maintenance of rickettsia raoultii in a rhipicephalus sanguineus tick cell line.rickettsia raoultii, a member of the spotted fever group rickettsiae, has been implicated in cases of debonel/tibola/senlat, and has been detected in dermacentor spp. and rhipicephalus pumilio ticks by pcr. r. raoultii has been isolated in mammalian and tick cell lines. this study aimed to isolate r. raoultii from spanish dermacentor marginatus in tick cell lines. a single adult d. marginatus collected from vegetation in la rioja (northen spain) in october 2012 was surface-sterilised, triturated ...201626428859
notes from the field: community-based prevention of rocky mountain spotted fever - sonora, mexico, 2016.rocky mountain spotted fever (rmsf), a life-threatening tickborne zoonosis caused by rickettsia rickettsii, is a reemerging disease in mexico (1,2). r. rickettsii is an intracellular bacterium that infects vascular endothelium and can cause multisystem organ failure and death in the absence of timely administration of a tetracycline-class antibiotic, typically doxycycline. epidemic rmsf, as described in parts of arizona and mexico, is associated with massive local infestations of the brown dog t ...201627880753
distribution of spotted fever group rickettsiae in hard ticks (ixodida: ixodidae) from panamanian urban and rural environments (2007-2013).tick-borne rickettsiosis is an important emerging disease in panama; to date, there have been 12 confirmed cases, including eight fatalities. to evaluate the distribution of rickettsiae in panamanian ticks, we collected questing and on-host ticks in urban and rural towns in elevations varying between 0 and 2300 m. a total of 63 sites (13 urban and 50 rural towns) were used to develop models of spatial distributions. we found the following tick species: rhipicephalus sanguineus s.l. (present in 5 ...201627068930
ectoparasites of free-roaming domestic cats in the central united states.free-roaming domestic cat (felis catus) populations serve as a valuable resource for studying ectoparasite prevalence. while they share a similar environment as owned cats, free-roaming cats do not receive routine veterinary care or ectoparasiticide application, giving insight into parasite risks for owned animals. we examined up to 673 infested cats presented to a trap-neuter-return (tnr) clinic in the central united states. ectoparasite prevalences on cats were as follows: fleas (71.6%), ticks ...201627692321
manual for maintenance of multi-host ixodid ticks in the laboratory.use of laboratory animals as hosts for blood-sucking arthropods remains a time-proven and the most efficient method for establishment and propagation of slowly feeding ixodid ticks, despite introduction of techniques involving artificial feeding on either animal skins or synthetic membranes. new zealand white rabbits are usually the most accessible and most suitable hosts routinely used for establishment and maintenance of a large variety of multi-host tick species. here we describe standard pro ...201627651325
new records of ticks (acari: ixodidae) from dogs, cats, humans, and some wild vertebrates in alaska: invasion potential.during 2010-2016, tick specimens were solicited from veterinarians, biologists, and members of the public in alaska. eight species of ticks were recorded from domestic dogs. some ticks were collected from dogs with recent travel histories to other countries or other u.s. states, which appears to explain records of ticks not native to alaska such as amblyomma americanum (l.) (lone star tick), ixodes scapularis (say) (blacklegged tick), and ixodes ricinus (l.). however, we recorded dermacentor var ...201627524823
repellency to ticks (acari: ixodidae) of extracts of nigella sativa (ranunculaceae) and the anti-inflammatory dogsbestfriend™.motivated by observations that the canine anti-inflammatory cream dogsbestfriend™ (dbf) appeared to deter flies, mosquitoes, and ticks from treated animals, repellent efficacy bioassays using four species of ticks were conducted with three extracts of nigella sativa l. (ranunculaceae), a constituent of dbf. the dbf cream was tested against nymphs of lone star tick, amblyomma americanum (l.). in vertical filter paper assays, the three extracts applied at 0.413 mg extract/cm(2) filter paper repell ...201627394440
determination of the effective dose of a novel oral formulation of sarolaner (simparica™) for the treatment and month-long control of fleas and ticks on dogs.three laboratory studies were conducted to determine the appropriate dose of sarolaner, a novel isoxazoline, for the treatment and month-long control of infestations of fleas and ticks on dogs. in the first study, dogs were treated orally with sarolaner suspension formulations at 1.25, 2.5 or 5.0mg/kg, and infested with dermacentor reticulatus, rhipicephalus sanguineus ticks and with ctenocephalides felis felis (cat flea) prior to treatment and then weekly for up to 8 weeks. fleas and ticks were ...201626948830
efficacy of a novel oral formulation of sarolaner (simparica™) against five common tick species infesting dogs in the united states.the efficacy of a single oral treatment with sarolaner (simparica™, zoetis), a novel isoxazoline compound, was evaluated against five tick species known to infest dogs in the united states. a total of 10 laboratory studies, two against each species, were conducted using adult purpose-bred mongrels or beagle dogs. in each study, 16 dogs were randomly allocated to one of two treatment groups based on pre-treatment host-suitability tick counts. dogs were infested with approximately 50 unfed adult a ...201626935819
ticks associated with domestic dogs and cats in florida, usa.voluntary collections of ticks from domestic dogs and cats by veterinary practitioners across florida, usa, were conducted over a 10 month period. of the 1337 ticks submitted, five species of ixodid ticks were identified and included rhipicephalus sanguineus, amblyomma americanum, a. maculatum, dermacentor variabilis, and ixodes scapularis. most ticks were collected from dogs (98.4%) with the most predominant species being r. sanguineus (94.3%). of the ticks collected from cats (1.6%), a. americ ...201626888081
the putative role of rhipicephalus microplus salivary serpins in the tick-host relationship.inflammation and hemostasis are part of the host's first line of defense to tick feeding. these systems are in part serine protease mediated and are tightly controlled by their endogenous inhibitors, in the serpin superfamily (serine protease inhibitors). from this perspective ticks are thought to use serpins to evade host defenses during feeding. the cattle tick rhipicephalus microplus encodes at least 24 serpins, of which rms-3, rms-6, and rms-17 were previously identified in saliva of this ti ...201626844868
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