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canine leishmaniosis in south america.abstract : canine leishmaniosis is widespread in south america, where a number of leishmania species have been isolated or molecularly characterised from dogs. most cases of canine leishmaniosis are caused by leishmania infantum (syn. leishmania chagasi) and leishmania braziliensis. the only well-established vector of leishmania parasites to dogs in south america is lutzomyia longipalpis, the main vector of l. infantum, but many other phlebotomine sandfly species might be involved. for quite som ...200919426440
tick-borne zoonotic bacteria in ticks collected from central spain.the prevalence of tick-borne and related bacteria infecting adult ticks in central spain was assessed by molecular methods. six areas were sampled monthly during a 2-year longitudinal study. a total of 1,038 questing and 442 feeding ticks, belonging to eight different species, were tested. the most abundant species were hyalomma lusitanicum (54% of captures), followed by dermacentor marginatus (23%) and rhipicephalus sanguineus (10%). four human pathogens, including seven rickettsia species, ana ...200919556569
acaricidal efficacy of thymol on engorged nymphs and females of rhipicephalus sanguineus (latreille, 1808) (acari: ixodidae).the present work was aimed at evaluating the "in vitro" efficacy of different concentrations of thymol on engorged nymphs and females of rhipicephalus sanguineus. the nymphs were separated in seven groups and immersed in different concentrations of thymol (0.25%, 0.5%, 1.0%, 1.5%, and 2.0%) for 5 min. a control group was established (water + dimethylsulfoxide) together with a positive control group (amitraz*), and mortality was evaluated after 15 days. in order to perform the experiment with fem ...200919562380
attachment of nymphal rhipicephalus sanguineus (acari: ixodidae) to a human in an urban area followed by severe adverse reaction shortly before drop-off.a case of attachment and complete engorgement of a rhipicephalus sanguineus (latreille, 1806) nymph on a woman with severe pain shortly before nymphal drop-off is described. the pain continued for about 2 weeks after tick removal. apparently, this is the first documented case of human adverse reaction developed at the very last stage of engorgement of nymphal r. sanguineus. the infestation most likely took place inside the enclosed household garden in the southern area of jerusalem where the wom ...200919391333
emerging arthropod-borne diseases of companion animals in europe.vector-borne diseases are caused by parasites, bacteria or viruses transmitted by the bite of hematophagous arthropods (mainly ticks and mosquitoes). the past few years have seen the emergence of new diseases, or re-emergence of existing ones, usually with changes in their epidemiology (i.e. geographical distribution, prevalence, and pathogenicity). the frequency of some vector-borne diseases of pets is increasing in europe, i.e. canine babesiosis, granulocytic anaplasmosis, canine monocytic ehr ...200919403239
canine babesiosis caused by babesia canis vogeli in rural areas of the state of minas gerais, brazil and factors associated with its seroprevalence.this epidemiological survey on canine babesiosis was carried out in three distinct rural regions (lavras, belo horizonte and nanuque) of the state of minas gerais, brazil. ticks and blood samples were collected during a dry season (lavras, n=92; belo horizonte, n=50; nanuque, n=102) and the subsequent rainy season (lavras, n=71; belo horizonte, n=28; nanuque, n=66) from dogs living on farms. plasma samples were analyzed by the indirect fluorescent antibody test for detection of anti-babesia cani ...200918723199
detection of coxiella burnetii in ticks collected from central spain.a total of 1482 adult ticks collected from vegetation and animals in central spain in 2003-2005 were tested for the presence of coxiella burnetii by polymerase chain reaction and subsequent reverse line blot hybridization (pcr-rlb). c. burnetii was identified in 7.7% of questing ticks (80/1039) and 3.4% of ticks collected from animals (15/443) belonging to four species: hyalomma lusitanicum, dermacentor marginatus, rhiphicephalus sanguineus, and r. pusillus. these findings show an active role of ...200918945192
identification, functional characterization and expression patterns of a water-specific aquaporin in the brown dog tick, rhipicephalus sanguineus.much is known about the physiology of tick salivation, but nothing is known about the movement of water through the cell membranes of salivary glands, a phenomenon usually associated with water channels or aquaporins (aqps). an aqp, rsaqp1, was identified in a salivary gland cdna library of rhipicephalus sanguineus. in the first functional characterization of an acarine aqp, xenopus oocytes expressing rsaqp1 became water permeable, whereas rsaqp1 did not transport glycerol or urea. rsaqp1 was in ...200919000768
antibody responses of domestic animals to salivary antigens of triatomainfestans as biomarkers for low-level infestation of triatomines.hematophagous arthropods such as triatoma infestans, the vector of trypanosoma cruzi, elicit host-immune responses during feeding. characterization of antibody responses to salivary antigens offers the potential to develop immunologically based monitoring techniques for exposure to re-emergent triatomine bug populations in peridomestic animals. igg-antibody responses to the salivary antigens of t.infestans have been detected in chickens as soon as 2 days after the first exposure to five adult bu ...200919248784
the relationship between spotted fever group rickettsiae and ixodid ticks.spotted fever group rickettsiae are predominantly transmitted by ticks. rickettsiae have developed many strategies to adapt to different environmental conditions, including those within their arthropod vectors and vertebrate hosts. the tick-rickettsiae relationship has been a point of interest for many researchers, with most studies concentrating on the role of ticks as vectors. unfortunately, less attention has been directed towards the relationship of rickettsiae with tick cells, tissues, and ...200919358804
toward diagnosing leishmania infantum infection in asymptomatic dogs in an area where leishmaniasis is endemic.the most frequently used diagnostic methods were compared in a longitudinal survey with leishmania infantum-infected asymptomatic dogs from an area of italy where leishmaniasis is endemic. in february and march 2005, 845 asymptomatic dogs were tested by an immunofluorescence antibody test (ifat), a dipstick assay (ds), and an enzyme-linked immunosorbent assay (elisa) for l. infantum and by ifat for ehrlichia canis. dogs seronegative for l. infantum were further parasitologically evaluated by mic ...200919129471
prevalence of rhipicephalus sanguineus ticks on dogs in a region on the mexico-usa border. 200919136688
transmission of cytauxzoon felis to a domestic cat by amblyomma americanum.cytauxzoon felis was transmitted to a domestic cat by amblyomma americanum. the infection was produced by the bite of a. americanum adults that were acquisition fed as nymphs on a domestic cat that naturally survived infection of c. felis. fever, inappetence, depression, and lethargy were first noted 11 days post-infestation (dpi). pale mucus membranes, splenomegaly, icterus, and dyspnea were also observed during the course of the disease. the body temperature of the experimentally infected c. f ...200919168288
molecular analysis of ixodes granulatus, a possible vector tick for borrelia burgdorferi sensu lato in taiwan.the genetic identity of ixodes granulatus ticks was determined for the first time in taiwan. the phylogenetic relationships were analyzed by comparing the sequences of mitochondrial 16s ribosomal dna gene obtained from 19 strains of ticks representing seven species of ixodes and two outgroup species (rhipicephalus sanguineus and haemaphysalis inermis). four major clades could be easily distinguished by neighbour-joining analysis and were congruent by maximum-parsimony method. all these i. granul ...200919184580
rocky mountain spotted fever in dogs, brazil.clinical illness caused by rickettsia rickettsii in dogs has been reported solely in the united states. we report 2 natural clinical cases of rocky mountain spotted fever in dogs in brazil. each case was confirmed by seroconversion and molecular analysis and resolved after doxycycline therapy.200919239764
infection and replication of bartonella species within a tick cell line.bartonella species are fastidious, gram negative bacteria, some of which are transmitted by arthropod vectors, including fleas, sandflies, and lice. there is very little information regarding the interaction and/or transmission capabilities of bartonella species by ticks. in the present study, we demonstrate successful infection of the amblyomma americanum cell line, aae12, by seven bartonella isolates and three candidatus bartonella species by electron or light microscopy. with the exception of ...200919242658
allopatric speciation in ticks: genetic and reproductive divergence between geographic strains of rhipicephalus (boophilus) microplus.the cattle tick, rhipicephalus (boophilus) microplus, economically impact cattle industry in tropical and subtropical regions of the world. the morphological and genetic differences among r. microplus strains have been documented in the literature, suggesting that biogeographical and ecological separation may have resulted in boophilid ticks from america/africa and those from australia being different species. to test the hypothesis of the presence of different boophilid species, herein we perfo ...200919243585
experimental transmission of field anaplasma marginale and the a. centrale vaccine strain by hyalomma excavatum, rhipicephalus sanguineus and rhipicephalus (boophilus) annulatus ticks.the cattle rickettsia anaplasma marginale is distributed worldwide and is transmitted by about 20 tick species, but only rhipicephalus simus, a strictly african tick species, has been shown to transmit the vaccine strain of a. centrale. the aim of the present study was to examine transmission of field strains of a. marginale and of the vaccine strain of a. centrale by three tick species -hyalomma excavatum, rhipicephalus sanguineus and rhipicephalus (boophilus) annulatus - to susceptible calves. ...200918823724
rickettsia helvetica in dermacentor reticulatus ticks.we report on the molecular evidence that dermacentor reticulatus ticks in croatia are infected with rickettsia helvetica (10%) or rickettsia slovaca (2%) or co-infected with both species (1%). these findings expand the knowledge of the geographic distribution of r. helvetica and d. reticulatus ticks.200919116063
conservation and immunogenicity of the mosquito ortholog of the tick-protective antigen, subolesin.the control of arthropod vectors of pathogens that affect human and animal health is important for the eradication of vector-borne diseases. the ortholog of the tick-protective antigen, subolesin, was identified in aedes albopictus and found to have conserved epitopes in ticks and mosquitoes. rna interference with the tick and mosquito double-stranded rna in three tick species resulted in significant gene knockdown and decreased tick weight and/or survival. feeding anopheles atroparvus, aedes ca ...200919229557
79-year-old man with fever, malaise, and jaundice. 200919252117
molecular documentation of bartonella infection in dogs in greece and italy.this study reports the occurrence of "bartonella rochalimae" in europe and the presence of bartonella vinsonii subsp. berkhoffii genotypes ii and iii in dogs in southern italy and provides dna sequencing evidence of a potentially new bartonella sp. infecting dogs in greece and italy.200919261798
mediterranean spotted fever in algeria--new trends.mediterranean spotted fever (msf) due to rickettsia conorii is the most important tick-borne disease occurring in north africa. however, there are only a few fragmentary reports on the epidemiology and clinical aspects of rickettsioses in north africa, and cases are still rarely documented. we report herein a prospective study conducted in oran, the second largest city in algeria. this disease has not been properly described in oran or in other algerian cities.200918930677
effects of climate change on ticks and tick-borne diseases in europe.zoonotic tick-borne diseases are an increasing health burden in europe and there is speculation that this is partly due to climate change affecting vector biology and disease transmission. data on the vector tick ixodes ricinus suggest that an extension of its northern and altitude range has been accompanied by an increased prevalence of tick-borne encephalitis. climate change may also be partly responsible for the change in distribution of dermacentor reticulatus. increased winter activity of i ...200919277106
a novel sphingomyelinase-like enzyme in ixodes scapularis tick saliva drives host cd4 t cells to express il-4.tick feeding modulates host immune responses. tick-induced skewing of host cd4(+) t cells towards a th2 cytokine profile facilitates transmission of tick-borne pathogens that would otherwise be neutralized by th1 cytokines. tick-derived factors that drive this th2 response have not previously been characterized. in the current study, we examined an i. scapularis cdna library prepared at 18-24 h of feeding and identified and expressed a tick gene with homology to loxosceles spider venom proteins ...200919292772
burden of tick-borne infections on american companion animals.this review examines the biology of ticks and tick-borne infections in the united states. the most common tick-borne diseases in dogs and cats are discussed. we demonstrate that there is much interest in tick-borne infections at the level of the lay public (pet owners), describe trends in the distribution and prevalence of tick-borne infections in the united states, summarize some issues in understanding the degree of ill health due to tick-borne infections, and suggest some avenues for research ...200919945085
when yellow jackets attack: recurrent and severe anaphylactic reactions to insect bites and stings. 200919862831
effects of fipronil (active ingredient of frontline) on salivary gland cells of rhipicephalus sanguineus females (latreille, 1806) (acari: ixodidae).the present study analyzed the effects of the chemical compound fipronil, active ingredient of frontline (acaricide and insecticide), on the salivary glands of unfed and semi-engorged female rhipicephalus sanguineus tick. unfed females were only exposed to the concentration of 1 ppm of fipronil, while semi-engorged females were treated with fipronil in three concentrations: 1 ppm, 5 ppm, and 10 ppm (distilled water was used as control). the histological and histochemical results revealed signifi ...200919748741
a survey of ectoparasitic arthropods on domestic animals in tak province, thailand.in july 2008 a survey of ectoparasites on domestic animals was conducted in the royal thai army areas of operation along the thai-myanmar border, tak province, thailand. eleven different ectoparasites were collected: two species of hard ticks (ixodidae), three species of fleas (siphonaptera) and 6 species of sucking or chewing lice (2 species each in the suborders anoplura, ischnocera and amblycera) were collected. domestic dogs (canis lupusfamiliaris) (n = 94) were found infested with 2 species ...200919842427
prevalence and associated risk factors for bovine tick infestation in two districts of lower punjab, pakistan.bovine tick infestation is still a serious nuisance to livestock and the dairy industry of pakistan. the current paper reports the prevalence and associated risk factors for bovine tick infestation in the districts layyah and muzaffargarh of lower punjab, pakistan. a questionnaire-based survey was conducted to identify and to quantify variation in the prevalence of bovine tick infestation with respect to host (age, species, sex, and breed) and environmental (geographical area and climate) determ ...200919782414
seasonal variation of infestation rate with lice, tick and mite among rodents in certain egyptian regions.four species of mites (ornithonyssus bacoti, haemolaelaps glusgowi, echinolaelaps echinolelaps & dermanyssus gallinae), two species of ticks (rhipicephalus sanguineus & hyalomma dromedarrii) and one species of lice (polyplax spinulose) were identified on rodents during 4 successive seasons (2005) trapped in five governorates (suez, menoufia, giza, damietta and beni-sewaf). the rodents were rattus norvegicus, rattus rattus alexandrinus, rattus rattus frugivourus, acomys cahirinus and mus musculus ...200919795768
ehrlichia chaffeensis infections in drosophila melanogaster.ehrlichia chaffeensis is an obligate, intracellular bacterium, transmitted by the tick amblyomma americanum, and is the causative agent of human monocytic ehrlichiosis infections. we previously demonstrated that e. chaffeensis is capable of growing in drosophila s2 cells. therefore, we tested the hypothesis that e. chaffeensis can infect adult drosophila melanogaster. adult drosophila organisms were experimentally challenged with intra-abdominal injections of bacteria. ehrlichia-infected flies s ...200919687202
ectoparasites of dogs and cats in albania.one hundred eighty-one dogs and 26 short-hair cats from suburban areas around tirana, albania were examined for ectoparasite infestation. the dogs were examined on several occasions from 2005 through 2009 representing three seasons: winter (december-february), spring (march-may), and summer (june-august); the cats were examined in late autumn (november). in addition, deep ear swab specimens of 30 dogs were examined for ear mites. the arthropod ectoparasite fauna of the dogs included two tick spe ...200919690887
preliminary study on the acaricidal efficacy of spinosad administered orally to dogs infested with the brown dog tick, rhipicephalus sanguineus (latreille, 1806) (acari: ixodidae).spinosad is a novel mode of action insecticide and acaricide derived from a family of natural compounds produced from fermentation of the actinomycete, saccharopolyspora spinosa. although spinosad has been shown to have rapid knockdown and 1 month of residual efficacy against fleas (ctenocephalides spp.) following oral administration in dogs, potential activity against ticks infesting dogs has not been determined. to address this possibility, a proof-of-concept laboratory efficacy study was cond ...200919713042
molecular and clinical evidence of ehrlichia chaffeensis infection in cameroonian patients with undifferentiated febrile illness.in the u.s.a., human monocytotropic ehrlichiosis (hme) caused by ehrlichia chaffeensis is an emerging tick-transmitted zoonosis. in cameroon, where e. canis, e. chaffeensis and e. ewingii have recently been detected in dogs and/or ticks (rhipicephalus sanguineus), the potential exists for human infections. patients from the coastal region of cameroon who had acute fevers of unknown aetiology were therefore checked for ehrlichial infection, using a real-time pcr that amplifies part of a genus-spe ...200920030996
ticks on domestic animals in pernambuco, northeastern brazil.the objective of this article was to discuss some aspects of ticks associated with domestic animals in the state of pernambuco, northeastern brazil, based on a literature review and present new data obtained from recent tick collections carried out in this northeastern brazilian state. from august 2007 to june 2008, 1,405 ticks were collected and five species were identified: amblyomma cajennense (fabricius, 1787), amblyomma ovale koch, 1844, dermacentor nitens neumann, 1897, rhipicephalus (boop ...200919772771
determination of lc(50) of permethrin acaricide in semi-engorged females of the tick rhipicephalus sanguineus (latreille, 1806) (acari: ixodidae).currently, the most efficient and widely used method of tick control still is the treatment with acaricides, especially permethrin (active ingredient of the advantage, max3, bayer), a pyrethroid with neurotoxic action. due to the wide use of this acaricide in the control of the tick rhipicephalus sanguineus, this study carried out laboratorial procedures to determine the lc(50) (lethal concentration fifty) of permethrin in semi-engorged females of r. sanguineus. based on the result of 14 dilutio ...200919679129
directed evolution of a filamentous fungus for thermotolerance.filamentous fungi are the most widely used eukaryotic biocatalysts in industrial and chemical applications. consequently, there is tremendous interest in methodology that can use the power of genetics to develop strains with improved performance. for example, metarhizium anisopliae is a broad host range entomopathogenic fungus currently under intensive investigation as a biologically based alternative to chemical pesticides. however, it use is limited by the relatively low tolerance of this spec ...200919709419
ticks and haemoparasites of dogs from praia, cape verde.in february 2008 an epidemiological field study on arthropod-borne infections in dogs was carried out in praia, the capital city of cape verde. for this purpose 130 dogs were included in the study. of these, 94.6% were infested with ticks. altogether, 1293 ticks of the genus rhipicephalus (in all evaluated cases r. sanguineus) were collected. examination for haemotropic parasites was performed via polymerase chain reaction (pcr). lymph node fine-needle aspirates were screened by pcr for leishman ...200919729247
tick-borne rickettsiosis in traveler returning from honduras. 200919751607
microarray analysis of gene expression changes in feeding female and male lone star ticks, amblyomma americanum (l).a collection of est clones from female tick amblyomma americanum salivary glands was hybridized to rna from different feeding stages of female tick salivary glands and from unfed or feeding adult male ticks. in the female ticks, the expression patterns changed dramatically upon starting feeding, then changed again towards the end of feeding. on beginning feeding, genes possibly involved in survival on the host increased in expression as did many housekeeping genes. as feeding progressed, some of ...200919514082
external and intestinal parasites of pet dogs with reference to zoonotic toxocariasis.examination of 3000 pet dogs over two years at the veterinary teaching hospital, faculty of veterinary medicine revealed external and internal parasites. the external parasites were rhipicephalus sanguineus, ctenocephalus felis, sarcoptes canis, and sarcoptes scabiei. the internal parasites were echinococcus granulosus, dipylidium caninum, rare heterophyes heterophyes, and toxocara canis. toxocara eggs were detected in 9.83%. the results were discussed with reference to zoonotic toxocariasis.200919530631
canine piroplasmosis in italy: epidemiological aspects in vertebrate and invertebrate hosts.in italy, canine piroplasmosis is believed to be widespread, but few data are available on its presence in most areas. in 2005 and 2006, vertebrate and invertebrate hosts were investigated in central and northern regions of the country. microscopy on blood smears, molecular tools and serological tests were applied to 420 blood samples collected from dogs, in order to evaluate the presence of these protozoa and to identify possible risk factors. moreover, ticks were analyzed by molecular techniqu ...200919632786
detection and identification of rickettsial agents in ticks from domestic mammals in eastern panama.several outbreaks of rocky mountain spotted fever have occurred in recent years in colombian communities close to the border with panama. however, little is known about rickettsiae and rickettsial diseases in eastern panamanian provinces, the darien province and the kuna yala, located north of the endemic area in colombia. in 2007, 289 ticks were collected in several towns from dogs, horses, mules, cows, and pigs. dna was extracted from 124 dermacentor nitens, 64 rhipicephalus sanguineus, 43 amb ...200919645289
characterization of rickettsial diseases in a hospital-based population in central tunisia.in tunisia, 2 rickettsial groups, spotted fever group and typhus group, have been described since the beginning of the 20th century. mediterranean spotted fever (msf), also known as boutonneuse fever, caused by rickettsia conorii and transmitted by the dog tick rhipicephalus sanguineus, is the most frequent rickettsial infection observed. its seroprevalence in our region is 9% among blood donors and 23% in hospitalized febrile patients. typhus group rickettsioses, caused by r. typhi and r. prowa ...200919538277
hepatozoon canis infecting dogs in the state of espírito santo, southeastern brazil.from may 2007 to march 2008, blood samples were collected from 92 healthy dogs living in 21 households (17 farms in rural area, and 4 homes in urban area) in 6 counties of the state of espírito santo, southeastern brazil. in addition, ticks were collected from these dogs. a mean of 4.4+/-3.0 dogs (range: 1-12) were sampled per household; 78 and 14 dogs were from rural and urban areas, respectively. polymerase chain reaction (pcr) designed to amplify fragments of the 18s rdna gene of babesia spp ...200919482427
[pediatric mediterranean spotted fever].in france, mediterranean spotted fever due to rickettsia conorii is mainly observed in the south-east. rhipicephalus sanguineus, the brown dog tick, is the vector and the reservoir of the disease. clinical diagnosis relies on the association of fever, rash and inoculation's scar during summertime. pathological lesion is a diffuse vasculitis. severity is often explained by a delay in diagnosis, but prognosis is good in pediatrics. treatment relies on doxycycline and on macrolids in children under ...200919836684
a dog naturally infected with rickettsia akari in yucatan, méxico.rickettsia akari is the causative agent of rickettsialpox, a primarily urban mite-borne rickettsiosis that is encountered in the united states and in a few countries around the world. its vector is the mite liponyssoides sanguineus, which is found on rats and mice, which serve as reservoirs for the disease. in this work we report a severe animal case of r. akari infection with two unusual features: r. akari was found in a dog, and its potential vector was a tick.200919492943
epidemiology and phylogenetic analysis of crimean-congo hemorrhagic fever viruses in xinjiang, china.in 2004 and 2005, an epidemiological survey of crimean-congo hemorrhagic fever virus (cchfv) was conducted in xinjiang, china. a total of 5,629 serum samples of human and livestock were collected and tested for the cchfv antibody, and 17,319 ticks were collected for viral identification. reverse passive hemagglutination inhibition assays showed that the average prevalence of cchfv antibody was 1.7% for the humans and 12.7% for the livestock. a relatively high antibody prevalence, ranging from 19 ...200919553586
the immunomodulatory action of sialostatin l on dendritic cells reveals its potential to interfere with autoimmunity.sialostatin l (sialol) is a secreted cysteine protease inhibitor identified in the salivary glands of the lyme disease vector ixodes scapularis. in this study, we reveal the mechanisms of sialol immunomodulatory actions on the vertebrate host. lps-induced maturation of dendritic cells from c57bl/6 mice was significantly reduced in the presence of sialol. although ova degradation was not affected by the presence of sialol in dendritic cell cultures, cathepsin s activity was partially inhibited, l ...200919494265
incongruent effects of two isolates of rickettsia conorii on the survival of rhipicephalus sanguineus ticks.rickettsia conorii, the etiologic agent of mediterranean spotted fever is widely distributed in southern europe, the middle east, africa, india and the caspian region. in the mediterranean region, the brown dog tick, rhipicephalus sanguineus, is the recognized vector of r. conorii. to study tick-pathogen relationships and pathogenesis of infection caused in model animals by the bite of an infected tick, we attempted to establish a laboratory colony of rh. sanguineus persistently infected with r. ...200919421877
[mutagenic occurrence in teleoginae of rhipicephalus sanguineus from intradomiciliary environment at rio de janeiro city].from material collected by into a residence house at rio de janeiro city, using an aspirator machine during four continues hours in all house, it was captured 1,284 rhipicephalus sanguineus including 289 females; one of them showed a mutagenic alteration like an asymmetry of opistosome, and monstrosity like a two anus. the first register of this phenomenon in r. sanguineus is reported.200919602319
influence of temperature on the attachment of rhipicephalus sanguineus ticks on rabbits. 200919438617
experimental infection of rhipicephalus sanguineus with rickettsia conorii conorii. 200919438618
new developments in canine hepatozoonosis in north america: a review.abstract : canine hepatozoonosis is caused by hepatozoon canis and hepatozoon americanum, apicomplexan parasites transmitted to dogs by ingestion of infectious stages. although the two agents are phylogenetically related, specific aspects, including characteristics of clinical disease and the natural history of the parasites themselves, differ between the two species. until recently, h. canis infections had not been clearly documented in north america, and autochthonous infection with h. america ...200919426444
closing the gaps between genotype and phenotype in rickettsia rickettsii.rocky mountain spotted fever (rmsf) caused by rickettsia rickettsii is a severe rickettsiosis that occurs in nearly every state of the continental usa. rmsf is endemic in central and southern america, with recent well-documented cases in mexico, costa rica, panama, colombia, brazil, and argentina. rmsf is the most malignant among known rickettsioses causing severe multiorgan dysfunction and high case fatality rates, which can reach 73% in untreated cases. variations in pathogenic biotypes of r. ...200919538260
transmission of rickettsia conorii conorii in naturally infected rhipicephalus sanguineus. 200919438619
rapid screening and cultivation of ehrlichia canis from refrigerated carrier blood. 200919438630
deciphering the relationships between rickettsia conorii conorii and rhipicephalus sanguineus in the ecology and epidemiology of mediterranean spotted fever.mediterranean spotted fever is the most important tick-borne disease occurring in southern europe and north africa. the first case of this life-threatening zoonosis was reported in 1910. in the 1930s, the role of the brown dog tick, rhipicephalus sanguineus, and the causative agent, rickettsia conorii were described. however, basic questions regarding the relationships between the rickettsia and its tick vector are still unresolved, and the life cycle of r. conorii is incompletely known. there i ...200919538263
structural basis of chemokine sequestration by a tick chemokine binding protein: the crystal structure of the complex between evasin-1 and ccl3.chemokines are a subset of cytokines responsible for controlling the cellular migration of inflammatory cells through interaction with seven transmembrane g protein-coupled receptors. the blocking of a chemokine-receptor interaction results in a reduced inflammatory response, and represents a possible anti-inflammatory strategy, a strategy that is already employed by some virus and parasites. anti-chemokine activity has been described in the extracts of tick salivary glands, and we have recently ...200920041127
mediterranean spotted fever, a diagnostic challenge in travellers.mediterranean spotted fever or boutonneuse fever is caused by rickettsia conorii and transmitted by the brown dog tick. the commonest symptoms are pyrexia, a maculopapular rash, lymphadenopathies and an inoculation eschar. increasingly, it is recognised as a cause of serious illness in southern europe. rickettsial infections of the spotted fever group are rarely reported in belgium. we report the case of a 20-year-old traveller returning from morocco who presented with fever and a markedly swoll ...200920101874
ectoparasite infestation on rural dogs in the municipality of são vicente férrer, pernambuco, northeastern brazil.this article presents the results of a study on ectoparasites infesting rural dogs in pernambuco, northeastern brazil. forty-one dogs from a rural community of pernambuco were examined and 70.7% were infested by ectoparasites. the prevalence rates of infestation by ticks, fleas, and lice were 58.5, 43.9 and 22%, respectively. of 24 dogs parasitized by ticks, 15 were exclusively infested by rhipicephalus sanguineus (latreille) (32 females, 66 males, 9 nymphs; prevalence, 48.8%; mean intensity, 5. ...200919772781
treatment with a novel chemokine-binding protein or eosinophil lineage-ablation protects mice from experimental colitis.eosinophils are multifunctional leukocytes implicated in numerous inflammatory diseases. the present study was conducted to clarify the precise role of eosinophils in the development of colitis by using eosinophil-depleted mice and a novel chemokine-binding protein that neutralizes ccl11 action. colitis was induced by administration of dextran sodium sulfate (dss) to wild-type and eosinophil-deficient deltadblgata-1 mice. accumulation of eosinophils in the gut of mice given dss paralleled worsen ...200919893035
fleatickrisk: a meteorological model developed to monitor and predict the activity and density of three tick species and the cat flea in europe.mathematical modelling is quite a recent tool in epidemiology. geographical information system (gis) combined with remote sensing (data collection and analysis) provide valuable models, but the integration of climatologic models in parasitology and epidemiology is less common. the aim of our model, called "fleatickrisk", was to use meteorological data and forecasts to monitor the activity and density of some arthropods. our parasitological model uses the weather research and forecasting (wrf) me ...200919908193
rickettsia spp. infection in rhipicephalus sanguineus ticks in a brazilian spotted fever endemic rural area in rio de janeiro state, brazil. 200920584167
toxicity of neem seed oil against the larvae of boophilus decoloratus, a one-host tick in cattle.the in vitro toxicity of neem seed oil (azadirachta indica a. juss, family: meliaceae, dogon yaro in hausa language in nigeria) was tested against the larvae of a one-host tick, boophilus decoloratus (family: ixodidae or hard tick, commonly known as blue tick) parasitic mainly to cattle generally found in savannas of tropical equatorial africa. the 20, 40, 60, 80 and 100% concentrations of neem seed oil were found to kill all (100% mortality) the larvae after 27, 27, 27, 27 and 24 h respectively ...200920502579
ticks (acari: ixodidae) infesting camels (camelus dromedarius) in northern sudan.monthly total body tick collections from 13-20 camels were conducted for 2 consecutive years (2000-2001) in northern sudan. tick populations were correlated with locality, season, predeliction site, sex and coat colour. hyalomma dromedarii was found to be the predominant (89%) tick species infesting the camels. other tick species found in very low numbers were hyalomma impeltatum (7.7%), hyalomma anatolicum anatolicum (3.3%), hyalomma truncatum (0.29%), hyalomma marginatum rufipes (0.25%), rhipi ...200920698438
babesia and its hosts: adaptation to long-lasting interactions as a way to achieve efficient transmission.babesia, the causal agent of babesiosis, are tick-borne apicomplexan protozoa. true babesiae (babesia genus sensu stricto) are biologically characterized by direct development in erythrocytes and by transovarial transmission in the tick. a large number of true babesia species have been described in various vertebrate and tick hosts. this review presents the genus then discusses specific adaptations of babesia spp. to their hosts to achieve efficient transmission. the main adaptations lead to lon ...200919379662
the complete mitochondrial genome of the house dust mite dermatophagoides pteronyssinus (trouessart): a novel gene arrangement among arthropods.the apparent scarcity of available sequence data has greatly impeded evolutionary studies in acari (mites and ticks). this subclass encompasses over 48,000 species and forms the largest group within the arachnida. although mitochondrial genomes are widely utilised for phylogenetic and population genetic studies, only 20 mitochondrial genomes of acari have been determined, of which only one belongs to the diverse order of the sarcoptiformes. in this study, we describe the mitochondrial genome of ...200919284646
molecular analysis of the rrna genes of babesia spp and ehrlichia canis detected in dogs from ribeirão preto, brazil.the partial dna sequences of the 18s rrna gene of babesia canis and the 16s rrna gene of ehrlichia canis detected in dogs from ribeirão preto, brazil, were compared to sequences from other strains deposited in genbank. the e. canis strain circulating in ribeirão preto is identical to other strains previously detected in the region, whereas the subspecies babesia canis vogeli is the main babesia strain circulating in dogs from ribeirão preto.200924031351
in vitro isolation and molecular characterization of an ehrlichia canis strain from são paulo, brazil.an ehrlichia canis isolate was obtained from an naturally infected dog exhibiting clinical signs of ehrlichiosis in são paulo municipality, state of são paulo, brazil. the isolate was characterized by pcr and dna sequencing of portions of the ehrlichial genes dsb, 16srrna, and p28. partial dsb and 16s rrna sequences were identical to three and five other e. canis strains, respectively, from different countries and continents (including north america, africa, asia and europe). conversely, the p28 ...200824031251
[cross-reaction evaluation of pcr-anaplasma platys positive dogs tested to anaplasma phagocytophilum antibodies by commercial elisa].anaplasma platys, agent of canine cyclic thrombocytopenia parasites exclusively dogs platelets. its probable vector is the tick rhipicephalus sanguineus. among the existent diagnostic methods, the most used include: morulae identification in blood smears; antibody detection by indirect immunofluorescence; or dna amplification by polymerase chain reaction (pcr). recently a new commercial elisa (idexx®), capable of detecting a. phagocytophilum antibodies, has been developed. according to the manuf ...200820059806
[molecular analysis of the antigenic sequence (sbm7462®) from bm86 of the rhipicephalus (boophilus) microplus and similarity with rhipicephalus sanguineus and hyalomma anatolicum anatolicum].the vaccination represents optimum method evaluated with effective cost to prevent economic losses and to increase the duration and quality of life of the production animals. . diverse vaccines are produced from the intestinal protein bm86 of the rhipicephalus. (b.) microplus. the knowledge of the conservation of the gene bm86 is very important to evaluate the vaccine efficiency and the possibility of reaction crossed between different species of ticks. samples of r. (b.) microplus come from dif ...200820059809
larval keys to the genera of ixodidae (acari) and species of ixodes (latreille) ticks established in california.in california, hard (ixodidae) ticks transmit at least 8 zoonotic disease agents (1 virus, 6 bacteria, 1 protozoan) to humans or other animals. the correct taxonomic identification of all 3 parasitic stages (larvae, nymphs, adults) of ticks is integral to understanding host-tick associations and disease dynamics, but immature ticks, especially the larvae, can be difficult to identify. here, we present larval keys to the 4 genera of ixodidae (dermacentorkoch, 1844; haemaphysaliskoch, 1844; ixodes ...200820027236
mediterranean spotted fever.rickettsial disease has recently undergone an important evolution, particularly in the field of molecular genetics. this development includes mediterranean spotted fever (msf), a tick-borne disease caused by rickettsia conorii. important changes have occurred in ecologic and epidemiologic comprehension of the disease, and in the occurrence of severe forms. however, many questions still remain, including the identity of the real vector and reservoir of r conorii, and whether other risk factors ex ...200818755388
laboratory maintenance of rickettsia rickettsii.this unit includes protocols for the laboratory maintenance of the obligate intracellular bacterium rickettsia rickettsii, including propagation in mammalian cell cultures, as well as isolation, counting, and storage procedures. regulations for working with r. rickettsii in biosafety level 3 containment are also discussed.200819016440
defining the immune response to ehrlichia species using murine models.pathogenic bacteria belonging to the family anaplasmataceae include species of the genera ehrlichia and anaplasma. ehrlichia chaffeensis, first known as the causative agent of human monocytic ehrlichiosis, also infects several vertebrate hosts including white-tailed deer, dogs, coyotes and goats. e. chaffeensis is transmitted from the bite of an infected hard tick, such as amblyomma americanum. e. chaffeensis and other tick-transmitted pathogens have adapted to both the tick and vertebrate host ...200819028013
fatal case of israeli spotted fever after mediterranean cruise. 200819046528
comparative susceptibility of larval stages of amblyomma aureolatum, amblyomma cajennense, and rhipicephalus sanguineus to infection by rickettsia rickettsii.the current study compared the susceptibility of larval stages of amblyomma cajennense (f.), amblyomma aureolatum (pallas), and rhipicephalus sanguineus (latreille) to infection by a brazilian strain of rickettsia rickettsii. guinea pigs experimentally infected by r. rickettsii were simultaneously infested by larvae of the three tick species. recovered engorged larvae were allowed to molt to nymphs and held in an incubator at 23 degrees c and 85-90% rh. subsequent flat nymphs were tested for ric ...200819058642
ticks produce highly selective chemokine binding proteins with antiinflammatory activity.bloodsucking parasites such as ticks have evolved a wide variety of immunomodulatory proteins that are secreted in their saliva, allowing them to feed for long periods of time without being detected by the host immune system. one possible strategy used by ticks to evade the host immune response is to produce proteins that selectively bind and neutralize the chemokines that normally recruit cells of the innate immune system that protect the host from parasites. we have identified distinct cdnas e ...200818678732
ectoparasites on reintroduced roe deer capreolus capreolus in israel.the ectoparasite fauna of reintroduced roe deer (capreolus capreolus) was surveyed in a mediterranean forest in israel. ectoparasites were collected from four female hand-reared deer during 2004 and 2005. seasonality, predilection sites of infestation, and the apparent effect of the parasites are presented. this is the first study of roe deer parasites in the east mediterranean. the ectoparasite fauna included three hippoboscid fly (lipoptena capreoli, hippobosca equina, and hippobosca longipenn ...200818689655
[mediterranean spotted fever: new trends and new questions]. 200818598863
a survey for infection with dirofilaria immitis, ehrlichia canis, borrelia burgdorferi, and babesia canis in feral and client-owned dogs in the turks and caicos islands, british west indies.the frequency of infection with dirofilaria immitis and babesia canis and seropositivity to ehrlichia canis and borrelia burgdorferi in feral and client-owned dogs was determined. feral dogs were 14.8 and 11.2 times more likely to be seropositive to d. immitis and e. canis, respectively, than were client-owned dogs. none of the dogs tested positive for b. burgdorferi or b. canis.200818624070
molecular survey of rickettsia spp. in sick dogs in italy.the aim of this retrospective study was to investigate the prevalence of rickettsia spp. dna in the blood of sick dogs from italy. canine blood samples (n=650) submitted for molecular testing of rickettsia spp. to a diagnostic laboratory from february 2003 to march 2006 were studied. the rickettsia spp. dna detection was performed by light cycler real-time pcr using hybridization probes separately conducted with specific primers and probes. the total percentage of rickettsia spp.-positive dog sa ...200818631232
new epidemiological data on brazilian spotted fever in an endemic area of the state of são paulo, brazil.the present work evaluated rickettsial infection in dogs and their ticks in an area endemic for brazilian spotted fever (bsf) in the metropolitan area of são paulo, brazil, where the tick amblyomma aureolatum was presumed to be the vector of the disease. ticks were collected on dogs from 185 houses, encompassing single infestations by rhipicephalus sanguineus, amblyomma aureolatum, amblyomma longirostre, or amblyomma sp. in dogs from 60 (32.4%), 77 (41.6%), 2 (1.1%), and 25 (13.5%) houses, respe ...200818847319
bartonella sp. bacteremia in patients with neurological and neurocognitive dysfunction.we detected infection with a bartonella species (b. henselae or b. vinsonii subsp. berkhoffii) in blood samples from six immunocompetent patients who presented with a chronic neurological or neurocognitive syndrome including seizures, ataxia, memory loss, and/or tremors. each of these patients had substantial animal contact or recent arthropod exposure as a potential risk factor for bartonella infection. additional studies should be performed to clarify the potential role of bartonella spp. as a ...200818632903
molecular method for discrimination between francisella tularensis and francisella-like endosymbionts.environmental studies on the distribution of francisella spp. are hampered by the frequency of francisella-like endosymbionts that can produce a misleading positive result. a new, efficient molecular method for detection of francisella tularensis and its discrimination from francisella-like endosymbionts, as well as two variants associated with human disease (unusual f. tularensis strain fnsp1 and f. tularensis subsp. novicida-like strain 3523), is described. the method is highly specific and se ...200818650358
spotted fever group rickettsia in ticks from southeastern spain natural parks.during an 8-years study, we collected from vegetation or domestic and wild mammals 1246 ticks (624 males, 511 females and 111 nymphs) belonging to 13 species in jaen province (andalusia) and we analyzed these ticks by pcr and sequencing for the presence of rickettsiae. specific rickettsiae dna was detected in 243 (19.5%) of the ticks tested. sequence analysis of amplicons of glta, ompa and ompb genes revealed that ixodes ricinus were infected with r. monacensis, including strain irs3, and r. hel ...200818677442
identification of a novel babesia sp. from a sable antelope (hippotragus niger harris, 1838).babesiosis in a sable antelope (hippotragus niger harris, 1838) was first reported in 1930; the parasite was named babesia irvinesmithi. recently, specimens from an adult sable that presented with a sudden onset of disease and that subsequently died during immobilization were submitted for molecular characterization. microscopic examination of thin blood smears revealed the presence of small piroplasms. dna was extracted from blood samples; the v4 variable region of the 18s rrna gene was amplifi ...200818508943
genetic and antigenic diversities of major immunoreactive proteins in globally distributed ehrlichia canis strains.the extent of knowledge regarding the diversity of globally distributed ehrlichia canis strains has been limited to information gained from a few evolutionarily conserved genes. in this study, e. canis strains from the united states (strain jake [us]), brazil (strain são paulo [br]), and israel (strain 611 [is] and ranana [is-r]) were used to examine the antigenic and genetic diversities of four well-characterized major immunoreactive protein genes/proteins. gp36 and gp200 were the most divergen ...200818480237
evaluation of cytotoxic effects of fipronil on ovaries of semi-engorged rhipicephalus sanguineus (latreille, 1806) (acari: ixodidae) tick female.the ovary of the rhipicephalus sanguineus dog tick female is structurally formed by oocytes in five different stages of development (from i to v) and attached to the gonad by the pedicel. the present study evaluated possible toxic effects of the acaricide fipronil (frontline) on ovaries of semi-engorged ticks. sixty partially fed females of r. sanguineus tick were distributed into four groups of 15 specimens each: i--non-treated; ii, iii and iv--treated with 1, 5 and 10 ppm of fipronil, respecti ...200818501493
toxicity of extract of magonia pubescens (sapindales: sapindaceae) st. hil. to control the brown dog tick, rhipicephalus sanguineus (latreille) (acari: ixodidae).the action of crude ethanol extract of the stem bark of the soapberry magonia pubescens st. hil. was studied upon larvae of the brown dog tick rhipicephalus sanguineus (latreille). tick larvae were obtained by maintaining gravid females in an incubator, after collecting them from naturally infested kennels. the tick larvae were placed in envelopes of filter paper impregnated with different concentrations of the extract dissolved in dimethylsulfoxide (dmso) and distilled water. four tests were re ...200818506302
host surveys, ixodid tick biology and transmission scenarios as related to the tick-borne pathogen, ehrlichia canis.the ehrlichioses have been subject to increasing interest from veterinary and public health perspectives, but experimental studies of these diseases and their etiologic agents can be challenging. ehrlichia canis, the primary etiologic agent of canine monocytic ehrlichiosis, is relatively well characterized and offers unique advantages and opportunities to study interactions between a monocytotropic pathogen and both its vertebrate and invertebrate hosts. historically, advances in tick-borne dise ...200818963493
markers of cell death in salivary glands of males of the tick rhipicephalus sanguineus (latreille, 1806) (acari, ixodidae).the salivary glands of rhipicephalus sanguineus males at stages: unfed (control), at day seven post-attachment, and at days three and seven post-detachment from the host were examined using methods of enzymatic analysis and cell viability. at these stages of feeding, different staining patterns were observed in the cells of type iv, iii, ii and i acini, which were affected by degeneration in this sequence. acid phosphatase reaction was inversely proportional to that of atpase, while atpase react ...200818514570
[control of rhipicephalus sanguineus (latrielle, 1806) (acari: ixodidae) in the kennel of the ufmg veterinary school, belo horizonte, minas gerais, brazil].the present work was aimed at proposing a control measure for rhipicephalus sanguineus in a naturally infected kennel of 72 m(2) of the ufmg veterinary school with 25 dogs of different breeds. a sensitivity test to acaricide products was applied in a sample of r. sanguineus. out of the acaricide products tested: 12.5% amitraz (product 1); 2% alfamethrin and 60% dichlorvos association (product 2); 5% deltamethrin (product 3) and the association between 77.6% trichlorfon, 1.0% coumaphos and 1.0% c ...200818554440
warmer weather linked to tick attack and emergence of severe rickettsioses.the impact of climate on the vector behaviour of the worldwide dog tick rhipicephalus sanguineus is a cause of concern. this tick is a vector for life-threatening organisms including rickettsia rickettsii, the agent of rocky mountain spotted fever, r. conorii, the agent of mediterranean spotted fever, and the ubiquitous emerging pathogen r. massiliae. a focus of spotted fever was investigated in france in may 2007. blood and tissue samples from two patients were tested. an entomological survey w ...200819015724
absence of wolbachia endobacteria in the non-filariid nematodes angiostrongylus cantonensis and a. costaricensis.abstract: the majority of filarial nematodes harbour wolbachia endobacteria, including the major pathogenic species in humans, onchocerca volvulus, brugia malayi and wuchereria bancrofti. these obligate endosymbionts have never been demonstrated unequivocally in any non-filariid nematode. however, a recent report described the detection by pcr of wolbachia in the metastrongylid nematode, angiostrongylus cantonensis (rat lungworm), a leading cause of eosinophilic meningitis in humans. to address ...200818801163
exploring the mialome of ticks: an annotated catalogue of midgut transcripts from the hard tick, dermacentor variabilis (acari: ixodidae).ticks are obligate blood feeders. the midgut is the first major region of the body where blood and microbes ingested with the blood meal come in contact with the tick's internal tissues. little is known about protein expression in the digestive tract of ticks. in this study, for analysis of global gene expression during tick attachment and feeding, we generated and sequenced 1,679 random transcripts (ests) from cdna libraries from the midguts of female ticks at varying stages of feeding.200819021911
ticks infesting domestic animals in northern greece.the tick species infesting grazing animals in the countryside of 11 prefectures in northern greece were investigated during april-july and september-december of consecutive years 2003-2006. a total of 3,249 (1,952 males, 1,297 females) adult ticks were collected from goats, sheep, cattle and dogs. ticks were identified as ixodes ricinus (44.57%), ixodes gibbosus (4.09%), rhipicephalus bursa (19.14%), rhipicephalus sanguineus, rhipicephalus turanicus (5.79%), hyalomma marginatum marginatum (12.40 ...200818563592
anaphylactic shock caused by tick bites. 200818564642
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