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animal models of tick-borne hemorrhagic fever viruses.tick-borne hemorrhagic fever viruses (tbhfv) are detected throughout the african and eurasian continents and are an emerging or re-emerging threat to many nations. due to the largely sporadic incidences of these severe diseases, information on human cases and research activities in general have been limited. in the past decade, however, novel tbhfvs have emerged and areas of endemicity have expanded. therefore, the development of countermeasures is of utmost importance in combating tbhfv as elim ...201325437041
an emerging tick-borne disease of humans is caused by a subset of strains with conserved genome structure.the prevalence of tick-borne diseases is increasing worldwide. one such emerging disease is human anaplasmosis. the causative organism, anaplasma phagocytophilum, is known to infect multiple animal species and cause human fatalities in the u.s., europe and asia. although long known to infect ruminants, it is unclear why there are increasing numbers of human infections. we analyzed the genome sequences of strains infecting humans, animals and ticks from diverse geographic locations. despite exten ...201325437207
pathogens in ticks collected from dogs in berlin/brandenburg, germany.tick-borne diseases are a major health risk for humans and dogs. in addition to collection and analysis of questing ticks, analysis of host-associated ticks for the presence of pathogens is a valuable method to gain insight into transmission patterns of tick-borne diseases.201425441762
borrelia burgdorferi (lyme disease). 201425452659
genetic variability of babesia parasites in haemaphysalis spp. and ixodes persulcatus ticks in the baikal region and far east of russia.to study babesia diversity in ixodid ticks in russia, ixodes persulcatus, haemaphysalis japonica, haemaphysalisconcinna, dermacentor silvarum, and dermacentor nuttalli ticks collected in the far east and baikal region were assayed for the presence of babesia spp. using nested pcr. in total, babesia dna was detected in 30 of the 1125 (2.7%) i. persulcatus, 17 of the 573 (3.0%) h. concinna, and 12 of the 543 (2.2%) h. japonica but was undetectable in any of the 294 analyzed dermacentor spp. partia ...201425460820
morphological differentiation of ixodes persulcatus and i. ricinus hybrid larvae in experiment and under natural conditions.the objective of the study was detection of hybrid larvae in ixodes persulcatus and ixodes ricinus cohabitation sites. to this end, the following three tasks were solved: interspecies crossing of ticks, evaluation of typical morphological signs of the hybrid larvae, and analysis of collected specimens from sites of sympatry. under experimental conditions, hybrid larvae of i. persulcatus (female) and i. ricinus (male) were obtained that differed from the parental species by the size of setae on t ...201525468762
[conjugated variability of spontaneous activity and behavioral response to olfactory stimuli in the taiga tick (ixodes persulcatus)].according to -the automatic tracing of the movement of ticks in a petri dish, motivational variability of the spontaneous activity and behavioral response of the taiga tick to olfactory stimuli was analyzed. in the studied sample, two groups of ticks that differ in the movement trajectory in the absence of stimulus were isolated, including ticks that mainly moved on the edge of the dish at maximum accessible height (group 1) and ticks that mainly moved at the bottom and wall of the dish (group 2 ...201625510114
antibodies against borrelia burgdorferi sensu lato among adults, germany, 2008-2011.to assess borrelia burgdorferi sensu lato (the cause of lyme borreliosis) seropositivity in germany, we tested serum samples from health survey (2008-2011) participants. seroprevalence was 5.8% among women and 13.0% among men; infection risk was highest among persons >60 years of age. public health interventions, including education about risk factors and preventive measures, are needed.201525531140
whole-genome sequence of borrelia garinii strain 935t isolated from ixodes persulcatus in south korea.we report here the genome sequence of borrelia garinii strain 935t isolated from ixodes persulcatus in south korea. the 1,176,739 bp (g+c content, 27.73%) genome consists of 1,194 coding regions, 4 rrna genes, and 33 aminoacyl-trna synthetase genes. this is the first whole-genome report of a korean borrelia species isolate.201425540341
complete genome sequencing of borrelia valaisiana and borrelia afzelii isolated from ixodes persulcatus ticks in western siberia.lyme disease, caused by bacteria of the borrelia burgdorferi sensu lato complex, is the most frequent tick-borne infection in eurasia. here, we report the complete genome sequence of the borrelia valaisiana tom 4006 and borrelia afzelii tom 3107 strains isolated from ixodes persulcatus ticks in western siberia.201425540343
tick-borne encephalitis virus subtypes emerged through rapid vector switches rather than gradual evolution.tick-borne encephalitis is the most important human arthropod-borne virus disease in europe and russia, with an annual incidence of about 13 thousand people. tick-borne encephalitis virus (tbev) is distributed in the natural foci of forest and taiga zones of eurasia, from the pacific to the atlantic coast. currently, there are three mutually exclusive hypotheses about the origin and distribution of tbev subtypes, although they are based on the same assumption of gradual evolution. recently, we h ...201425540692
louping ill virus (liv) in the far east.this study focused on finding, culturing, and identifying the biological and genetic characteristics of three louping ill virus (liv) strains in the south of the russian far east. the primorye-155-77 and primorye-20-79 virus strains were isolated from ixodes persulcatus ticks, and the primorye-185-91 strain was isolated from the blood of a person after a tick bite. according to the hemagglutination and neutralization tests, primorye-155-77, primorye-20-79 and primorye-185-91 had weak reactivity ...201525543207
rickettsial diseases and ectoparasites from military bases in japan.ectoparasitic arthropods are often vectors of rickettsiosis. we conducted a survey of ectoparasites on u.s. military facilities throughout japan with the use of specimens submitted by pest control, public health, and veterinary personnel. over 1,600 individual ectoparasites were collected. fifteen species were identified, including several significant vectors of human diseases such as scrub typhus and rickettsial spotted fevers. these ectoparasites included ctenocephalides felis , haemaphysalis ...201525548900
lyme borreliosis: a review of data on transmission time after tick attachment.lyme borreliosis is increasing rapidly in many parts of the world and is the most commonly occurring vector-borne disease in europe and the usa. the disease is transmitted by ticks of the genus ixodes. they require a blood meal at each stage of their life cycle and feed on a wide variety of wild and domestic animals as well as birds and reptiles. transmission to humans is incidental and can occur during visits to a vector habitat, when host mammals and their associated ticks migrate into the urb ...201425565881
tick-borne pathogens and the vector potential of ticks in china.ticks, as obligate blood-sucking ectoparasites, attack a broad range of vertebrates and transmit a great diversity of pathogenic microorganisms. they are considered second only to mosquitoes as vectors of human disease, and the most important vector of pathogens of domestic and wild animals. of the 117 described species in the chinese tick fauna, 60 are known to transmit one or more diseases: 36 species isolated within china and 24 species isolated outside china. moreover, 38 of these species ca ...201525586007
molecular characterization of a defensin gene from a hard tick, dermacentor silvarum.ticks are distributed worldwide and considered as vectors of many human diseases. tick defensins, a family of antimicrobial peptides, form the first line of defense against pathogens.201525588982
investigation of tick-borne bacteria (rickettsia spp., anaplasma spp., ehrlichia spp. and borrelia spp.) in ticks collected from andean tapirs, cattle and vegetation from a protected area in ecuador.ixodid ticks play an important role in the transmission and ecology of infectious diseases. information about the circulation of tick-borne bacteria in ticks is lacking in ecuador. our aims were to investigate the tick species that parasitize andean tapirs and cattle, and those present in the vegetation from the buffer zone of the antisana ecological reserve and cayambe-coca national park (ecuador), and to investigate the presence of tick-borne bacteria.201525616567
long-term monitoring and population dynamics of ixodid ticks in tomsk city (western siberia).monitoring of two tick species (ixodes persulcatus schulze 1930 and ixodes pavlovskyi pomerantsev) was performed in four city parks of tomsk, russia (camp garden, university park, southern cemetery, and polytechnic stadium) and in a control wild biotope in city environs (kolarovo) in 2002-2013. ticks were collected by flagging repeatedly after each 10 ± 1 days, starting from the disappearance of the snow cover till the end of tick activity (april-august). gradual penetration of both tick species ...201525633264
guideline for veterinary practitioners on canine ehrlichiosis and anaplasmosis in europe.canine ehrlichiosis and anaplasmosis are important tick-borne diseases with a worldwide distribution. information has been continuously collected on these infections in europe, and publications have increased in recent years. prevalence rates are high for ehrlichia and anaplasma spp. infections in dogs from different european countries. the goal of this article was to provide a practical guideline for veterinary practitioners on the diagnosis, treatment, and prevention of ehrlichiosis and anapla ...201525649069
accelerated phenology of blacklegged ticks under climate warming.the phenology of tick emergence has important implications for the transmission of tick-borne pathogens. a long lag between the emergence of tick nymphs in spring and larvae in summer should increase transmission of persistent pathogens by allowing infected nymphs to inoculate the population of naive hosts that can subsequently transmit the pathogen to larvae to complete the transmission cycle. in contrast, greater synchrony between nymphs and larvae should facilitate transmission of pathogens t ...201525688016
the lyme disease pathogen has no effect on the survival of its rodent reservoir host.zoonotic pathogens that cause devastating morbidity and mortality in humans may be relatively harmless in their natural reservoir hosts. the tick-borne bacterium borrelia burgdorferi causes lyme disease in humans but few studies have investigated whether this pathogen reduces the fitness of its reservoir hosts under natural conditions. we analyzed four years of capture-mark-recapture (cmr) data on a population of white-footed mice, peromyscus leucopus, to test whether b. burgdorferi and its tick ...201525688863
multilocus spacer analysis revealed highly homogeneous genetic background of asian type of borrelia miyamotoi.borrelia miyamotoi, a member of the relapsing fever group borreliae, was first isolated in japan and subsequently found in ixodes ticks in north america, europe and russia. currently, there are three types of b. miyamotoi: asian or siberian (transmitted mainly by ixodes persulcatus), european (ixodesricinus) and american (ixodesscapularis and ixodespacificus). despite the great genetic distances between b. miyamotoi types, isolates within a type are characterised by an extremely low genetic vari ...201525697887
borrelia miyamotoi infection in nature and in humans.borrelia miyamotoi is a relapsing fever borrelia group spirochete that is transmitted by the same hard-bodied (ixodid) tick species that transmit the agents of lyme disease. it was discovered in 1994 in ixodes persulcatus ticks in japan. b. miyamotoi species phylogenetically cluster with the relapsing fever group spirochetes, which usually are transmitted by soft-bodied (argasid) ticks or lice. b. miyamotoi infects at least six ixodes tick species in north america and eurasia that transmit lyme ...201525700888
influence of altitude on tick-borne encephalitis infection risk in the natural foci of the altai republic, southern siberia.the altai republic is a highly endemic area as far as tick-borne encephalitis (tbe) is concerned. the aim of the research was to study the effect of altitude on the risk of tick-borne encephalitis infection in the altai republic. the paper analyzes the following data: the study of ixodid ticks collected from the vegetation in 116 sites at the 200-2383m elevation above sea level in 2012-2014, tbe virus prevalence of these vectors, tick-bite incidence rate, and tbe incidence rate of the population ...201525748512
ngs population genetics analyses reveal divergent evolution of a lyme borreliosis agent in europe and asia.borrelia bavariensis is a recently described agent of lyme disease within the b. burgdorferi sensu lato species complex and exhibits a strong capacity for human pathogenicity. b. bavariensis strains are widely distributed in eurasia spanning the distribution range of the tick vectors ixodes persulcatus and i. ricinus. it has been suggested that b. bavariensis forms two populations, one of which arose through vector adaptation and geographic expansion. we have performed phylogenetic and populatio ...201525766392
tissue distribution of the ehrlichia muris-like agent in a tick vector.human pathogens transmitted by ticks undergo complex life cycles alternating between the arthropod vector and a mammalian host. while the latter has been investigated to a greater extent, examination of the biological interactions between microbes and the ticks that carry them presents an equally important opportunity for disruption of the disease cycle. in this study, we used in situ hybridization to demonstrate infection by the ehrlichia muris-like organism, a newly recognized human pathogen, ...201525781930
an investigation of binding ability of ixodes persulcatus schulze salp15 with lyme disease spirochetes.salp15, a 15-kda tick salivary gland protein, has several suppressive modes of activity against host immunity and plays a critical role in the transmission of lyme disease spirochetes in ixodes scapularis and ixodes ricinus, major vectors of lyme disease in north america and western europe. salp15 adheres to borrelia burgdorferi and specifically interacts with its outer surface protein c (ospc), protecting the spirochete from antibody-mediated cytotoxicity and facilitating infection in the mice. ...201525796479
complete genome sequence of borrelia afzelii k78 and comparative genome analysis.the main borrelia species causing lyme borreliosis in europe and asia are borrelia afzelii, b. garinii, b. burgdorferi and b. bavariensis. this is in contrast to the united states, where infections are exclusively caused by b. burgdorferi. until to date the genome sequences of four b. afzelii strains, of which only two include the numerous plasmids, are available. in order to further assess the genetic diversity of b. afzelii, the most common species in europe, responsible for the large variety ...201525798594
nairobi sheep disease virus rna in ixodid ticks, china, 2013. 025811222
identification of 24h ixodes scapularis immunogenic tick saliva proteins.ixodes scapularis is arguably the most medically important tick species in the united states. this tick transmits 5 of the 14 human tick-borne disease (tbd) agents in the usa: borrelia burgdorferi, anaplasma phagocytophilum, b. miyamotoi, babesia microti, and powassan virus disease. except for the powassan virus disease, i. scapularis-vectored tbd agents require more than 24h post attachment to be transmitted. this study describes identification of 24h immunogenic i. scapularis tick saliva prote ...201525825233
comparative analysis of the roles of ixodes persulcatus and i. trianguliceps ticks in natural foci of ixodid tick-borne borrelioses in the middle urals, russia.long-term studies on natural foci of ixodid tick-borne borrelioses (itbb) have been performed in chusovskoi district of perm region, the middle urals, where the vectors of these infections are represented by two ixodid tick species: the taiga tick ixodes persulcatus and many times less abundant vole tick i. trianguliceps. over 10 years, more than 6000 half-engorged ticks were collected from small forest mammals using the standard procedure, and 1027 i. persulcatus and 1142 i. trianguliceps ticks ...201525843812
natural hybridization of the ticks ixodes persulcatus and ixodes pavlovskyi in their sympatric populations in western siberia.hybridization of ticks of the genus ixodes has been described for several species under laboratory conditions although no molecular genetics evidence confirming interspecific hybridization in nature is available. we have designed a real time pcr targeted on nuclear (its2) and mitochondrial (cox1) markers to accurately identify tick species and to detect interspecific hybrids of ixodes persulcatus and ixodes pavlovskyi in their sympatric populations in western siberia. a survey of 783 individual ...201525858121
an invasive mammal (the gray squirrel, sciurus carolinensis) commonly hosts diverse and atypical genotypes of the zoonotic pathogen borrelia burgdorferi sensu lato.invasive vertebrate species can act as hosts for endemic pathogens and may alter pathogen community composition and dynamics. for the zoonotic pathogen borrelia burgdorferi sensu lato, the agent of lyme borreliosis, recent work shows invasive rodent species can be of high epidemiological importance and may support host-specific strains. this study examined the role of gray squirrels (sciurus carolinensis) (n = 679), an invasive species in the united kingdom, as b. burgdorferi sensu lato hosts. w ...201525888168
[genetic characterisation of powassan virus (powv) isolated from haemophysalis longicornis ticks in primorye and two strains of tick-borne encephalitis virus (tbev) (flaviviridae, flavivirus): alma-arasan virus (aav) isolated from ixodes persulcatus ticks in kazakhstan and malyshevo virus isolated from aedes vexans nipponii mosquitoes in khabarovsk kray].the complete genomes of the three tick-borne flaviviruses (genus flavivirus, fam. bunyaviridae) were sequenced: povassan virus (powv, strain leiv-3070prm, isolated from haemophysalis logicornis in primorsky krai, russia in 1977), alma-arasan virus (aav, strain leiv-1380kaz, isolated from ixodes persulcatus ticks in kazakhstan in 1977) and malyshevo virus (isolated from a pool of aedes vexans nipponii mosquitoes, in the khabarovsk krai, russia in 1978). it is shown that aav and malyshevo virus ar ...201425895206
comparison of two commercial tick-borne encephalitis virus igg enzyme-linked immunosorbent assays.despite the availability of protective vaccines, tick-borne encephalitis virus (tbev) infections have been increasingly reported to the european centre for disease prevention and control in the past 2 decades. since the diagnosis of tbev exposure relies on serological testing, we compared two commercial enzyme-linked immunosorbent assays (elisas), i.e., immunozym fsme igg assay (elisa-1) and euroimmun fsme vienna igg assay (elisa-2). both assays use whole tbev antigens, but they differ in viral ...201525924768
tick microbiome: the force within.ticks are obligate blood-feeders and serve as vectors of human and livestock pathogens worldwide. defining the tick microbiome and deciphering the interactions between the tick and its symbiotic bacteria in the context of tick development and pathogen transmission will likely reveal new insights and spawn new paradigms to control tick-borne diseases. descriptive observations on the tick microbiome that began almost a century ago serve as forerunners to the gathering momentum to define the tick m ...201525936226
length of tick repellency depends on formulation of the repellent compound (icaridin = saltidin®): tests on ixodes persulcatus and ixodes ricinus placed on hands and clothes.the present study had the aim to test the repellent potential of the compound icaridin = saltidin® against the tick species ixodes ricinus and ixodes persulcatus using different formulations of the compound. tests were done on backs of impregnated human hands, on impregnated linen cloth and versus impregnated dog hair. it was found that 1. ixodes persulcatus-the common eastern european, russian ixodes species is significantly sensitive to icaridin = saltidin® as i. ricinus protecting for the tes ...201525952705
babesia spp. in questing ticks from eastern poland: prevalence and species diversity.a total of 853 questing ixodes ricinus males, females, and nymphs and of 582 questing dermacentor reticulatus males and females were collected from vegetation on the territory of the lublin province (eastern poland). the ticks were examined for the presence of babesia by pcr detecting part of 18s ribosomal rna (rrna) gene and nuclear small subunit rrna (ss-rdna) for determining of babesia spp. and babesia microti, respectively. the overall incidence of babesia strains in i. ricinus ticks was 4.6 ...201525976982
the recent evolution of a maternally-inherited endosymbiont of ticks led to the emergence of the q fever pathogen, coxiella burnetii.q fever is a highly infectious disease with a worldwide distribution. its causative agent, the intracellular bacterium coxiella burnetii, infects a variety of vertebrate species, including humans. its evolutionary origin remains almost entirely unknown and uncertainty persists regarding the identity and lifestyle of its ancestors. a few tick species were recently found to harbor maternally-inherited coxiella-like organisms engaged in symbiotic interactions, but their relationships to the q fever ...201525978383
tick-borne encephalitis: a review of epidemiology, clinical characteristics, and management.tick-borne encephalitis is an infection of central nervous system caused by tick-borne encephalitis virus transmitted to humans predominantly by tick bites. during the last few decades the incidence of the disease has been increasing and poses a growing health problem in almost all endemic european and asian countries. most cases occur during the highest period of tick activity, in central europe mainly from april to november. tick-borne encephalitis is more common in adults than in children. cl ...201525984517
interactions between tick and transmitted pathogens evolved to minimise competition through nested and coherent networks.natural foci of ticks, pathogens, and vertebrate reservoirs display complex relationships that are key to the circulation of pathogens and infection dynamics through the landscape. however, knowledge of the interaction networks involved in transmission of tick-borne pathogens are limited because empirical studies are commonly incomplete or performed at small spatial scales. here, we applied the methodology of ecological networks to quantify >14,000 interactions among ticks, vertebrates, and path ...201525993662
study of a cohort of 1,886 persons to determine changes in antibody reactivity to borrelia burgdorferi 3 months after a tick bite.lyme borreliosis is a tick-borne disease caused by the bacterium borrelia burgdorferi. the most frequent clinical manifestation is a rash called erythema migrans. changes in antibody reactivity to b. burgdorferi 3 months after a tick bite are measured using enzyme-linked immunosorbent assays (elisas). one assay is based on native purified flagellum antigen (igg), and the other assay is based on a recombinant antigen called c6 (igg or igm). paired samples were taken at the time of a tick bite and ...201525994550
human granulocytic anaplasmosis.human granulocytic anaplasmosis, a deer tick-transmitted rickettsial infection caused by anaplasma phagocytophilum, is a common cause of undifferentiated fever in the northeast and upper midwest united states. patients are often initially diagnosed with a mild viral infection, and illness readily resolves in most cases. however, as many as 3% develop life-threatening complications and nearly 1% die from the infection. although coinfections with borrelia burgdorferi and babesia microti occur, the ...201525999228
[parasitological factors impeding the transmission of the agent of babesiosis (babesia microti) to man from the tick ixodes persulcatus].based on the analysis of own and literature data, it is concluded that the following ma- in permanent system of ecologicalarasitological factors prevents the effective vector functions of the tick i. persulcatus in transmission of b. microti: lack of distinct nymphs' anthropophily; small spontaneous invasion of hungry adults; a duration of the parasitic phase in humans is insufficient to complete the sporogonic development, because victims interrupt the phase. therefore, not excluding the possib ...201526016332
isolation and characterization of tick-borne encephalitis virus from ixodes persulcatus in mongolia in 2012.tick-borne encephalitis virus (tbev) is a zoonotic virus belonging to the genus flavivirus, in the family flaviviridae. the virus, which is endemic in europe and northern parts of asia, causes severe encephalitis. tick-borne encephalitis (tbe) has been reported in mongolia since the 1980s, but details about the biological characteristics of the endemic virus are lacking. in this study, 680 ticks (ixodes persulcatus) were collected in selenge aimag, northern mongolia, in 2012. nine mongolian tbev ...201526025267
chronic lyme disease: misconceptions and challenges for patient management.lyme disease, infection with the tick-borne spirochete borrelia burgdorferi, causes both specific and nonspecific symptoms. in untreated chronic infection, specific manifestations such as a relapsing large-joint oligoarthritis can persist for years, yet subside with appropriate antimicrobial therapy. nervous system involvement occurs in 10%-15% of untreated patients and typically involves lymphocytic meningitis, cranial neuritis, and/or mononeuritis multiplex; in some rare cases, patients have p ...201526028977
eco-epidemiology of borrelia miyamotoi and lyme borreliosis spirochetes in a popular hunting and recreational forest area in hungary.borrelia miyamotoi, the newly discovered human pathogenic relapsing fever spirochete, and borrelia burgdorferi sensu lato are maintained in natural rodent populations. the aim of this study was to investigate the natural cycle of b. miyamotoi and b. burgdorferi s.l. in a forest habitat with intensive hunting, forestry work and recreational activity in southern hungary.201526048373
vaccination with cyclin-dependent kinase tick antigen confers protection against ixodes infestation.among arthropods, ticks lead as vectors of animal diseases and rank second to mosquitoes in transmitting human pathogens. cyclin-dependent kinases (cdk) participate in cell cycle control in eukaryotes. cdks are serine/threonine protein kinases and these catalytic subunits are activated or inactivated at specific stages of the cell cycle. to determine the potential of using cdks as anti-tick vaccine antigens, hamsters were immunized with recombinant ixodes persulcatus cdk10, followed by a homolog ...201526073111
field and laboratory evaluations of the efficacy of deet repellent against ixodes ticks.the objective of this study was to clarify the efficacy of a currently available n,n-diethyl-m-toluamide (deet) repellent against tick species in japan. we performed 2 different field trials: "human trap," and "flag-dragging." in total, 482 ticks were collected from white flannel cloths in the field studies. the collected tick species were ixodes persulcatus and i. ovatus, which accounted for 5.3% and 94.7% of the ticks in the human trap test and 31.4% and 68.6% in the flag-dragging test, respec ...201626073735
neoehrlichiosis: an emerging tick-borne zoonosis caused by candidatus neoehrlichia mikurensis.candidatus neoehrlichia mikurensis is an emerging tick-borne pathogen causing a systemic inflammatory syndrome mostly in persons with underlying hematologic or autoimmune diseases. as it is neither well-known nor well-recognized, it might be misdiagnosed as recurrence of the underlying disease or as an unrelated arteriosclerotic vascular event. the pathogen is transmitted by hard ticks of the genus ixodes and is closely associated with rodents in which transplacental transmission occurs. transov ...201626081117
complete genome sequences of four european subtype strains of tick-borne encephalitis virus from eastern siberia, russia.three tick-borne encephalitis virus (tbev) strains were isolated from ixodes persulcatus ticks, and one was isolated from a shrew in the territory of eastern siberia (russia). the level of sequence identity compared to neudoerfl (the european prototype strain) is 97.2 to 97.3%.201526089416
detection and identification of rickettsia species in ixodes tick populations from estonia.a total of 1640 ticks collected in different geographical parts of estonia were screened for the presence of rickettsia species dna by real-time pcr. dna of rickettsia was detected in 83 out of 1640 questing ticks with an overall prevalence of 5.1%. the majority of the ticks infected by rickettsiae were ixodes ricinus (74 of 83), while 9 of the 83 positive ticks were ixodes persulcatus. for rickettsial species identification, a part of the citrate synthase glta gene was sequenced. the majority o ...201526096853
c6 peptide-based multiplex phosphorescence analysis (phosphan) for serologic confirmation of lyme borreliosis.a single-tier immunoassay using the c6 peptide of vlse (c6) from borrelia burgdorferi sensu stricto (bb) has been proposed as a potential alternative to conventional two-tier testing for the serologic diagnosis of lyme disease in the united states and europe.201526147441
comparative metagenomic profiling of symbiotic bacterial communities associated with ixodes persulcatus, ixodes pavlovskyi and dermacentor reticulatus ticks.ixodes persulcatus, ixodes pavlovskyi, and dermacentor reticulatus ticks inhabiting western siberia are responsible for the transmission of a number of etiological agents that cause human and animal tick-borne diseases. because these ticks are abundant in the suburbs of large cities, agricultural areas, and popular tourist sites and frequently attack people and livestock, data regarding the microbiomes of these organisms are required. using metagenomic 16s profiling, we evaluate bacterial commun ...201526154300
genetic variability of rickettsia spp. in ixodes persulcatus/ixodes trianguliceps sympatric areas from western siberia, russia: identification of a new candidatus rickettsia species.rickettsia spp. are the causative agents of a number of diseases in humans. these bacteria are transmitted by arthropods, including ixodid ticks. dna of several rickettsia spp. was identified in ixodes persulcatus ticks, however, the association of ixodes trianguliceps ticks with rickettsia spp. is unknown. in our study, blood samples of small mammals (n=108), unfed adult i. persulcatus ticks (n=136), and i. persulcatus (n=12) and i. trianguliceps (n=34) ticks feeding on voles were collected in ...201526190452
genetic diversity of ixodes pavlovskyi and i. persulcatus (acari: ixodidae) from the sympatric zone in the south of western siberia and kazakhstan.the most epidemiologically significant tick species in siberia involved in transmission of a large number of pathogens causing human infectious diseases is ixodes persulcatus. ixodes pavlovskyi, being more active, also poses epidemiological threats. these tick species share morphology, activity seasons and geographic distribution range. in this paper, we characterize the geographic and genetic structures of i. persulcatus and i. pavlovskyi populations inhabiting the southern part of western sibe ...201526201397
seroprevalence of lyme disease and associated risk factors in rural population of beijing.a seroepidemiological survey of 801 local residents from 28 villages was conducted to assess the seroprevalence of lyme disease and to identify the risk factors of becoming seropositive for lyme disease in the northern suburb of beijing. forty-one serum samples were positive for igg against b burgdorferi and the seroprevalence was 5.1% (41/801), indicating that lyme disease is endemic in the rural population. in the multivariable analysis, sowing and harvesting in summer (or, 2.377, 95% ci, 1.23 ...201526221360
the pcr detection and phylogenetic characterization of babesia microti in questing ticks in mongolia.babesia microti is a tick-transmitted zoonotic hemoprotozoan parasite. in the present study, we investigated b. microti infection in questing ticks in mongolia. a total of 219 questing ticks were collected from three different mongolian provinces (bayan-olgii, khovsgol, and selenge). of these, 63 from selenge were identified as ixodes persulcatus, while the remaining 156 (from all three provinces) were identified as dermacentor nuttalli. when the tick dna samples were screened using a b. microti ...201526225461
detection, identification and genotyping of borrellia spp. in rodents in slovenia by pcr and culture.borrelia burgdorferi sensu lato, the agent of lyme borreliosis, is mainly maintained in natural foci through the transmission cycles of competent tick vectors (ixodes sp.) and a vertebrate reservoir. specific rodents have been identified as the principal reservoir of borrelia burgdorferi sensu lato in europe. borrelia miyamotoi is the only relapsing fever spirochete transmitted by the same tick. the aim of the present study was to perform an epidemiological survey to determine the presence of b. ...201526253121
prevalence of candidatus rickettsia tarasevichiae-like bacteria in ixodid ticks at 13 sites on the chinese-russian border.to investigate the likely source population and candidate vectors of candidatus rickettsia tarasevichiae, the prevalence of this bacteria was quantified in specimens of four tick species that mainly parasitize humans collected from 13 sites along the chinese-russian border. the presence of the bacteria was determined by detecting its specific citrate synthase (glta) partial gene and outer membrane protein a (ompa) partial gene. only ixodes persulcatus schulze was found to be naturally infected w ...201426309321
opening the black box of anaplasma phagocytophilum diversity: current situation and future perspectives.anaplasma phagocytophilum is a zoonotic obligate intracellular bacterium known to be transmitted by ticks belonging to the ixodes persulcatus complex. this bacterium can infect several mammalian species, and is known to cause diseases with variable symptoms in many domestic animals. specifically, it is the causative agent of tick-borne fever (tbf), a disease of important economic impact in european domestic ruminants, and human granulocytic anaplasmosis (hga), an emerging zoonotic disease in asi ...201526322277
a broad-range survey of ticks from livestock in northern xinjiang: changes in tick distribution and the isolation of borrelia burgdorferi sensu stricto.borreliosis is highly prevalent in xinjiang uygur autonomous region, china. however, little is known about the presence of borrelia pathogens in tick species in this region, in addition borrelia pathogens have not been isolated from domestic animals.201526337627
effects of parasites and antigenic challenge on metabolic rates and thermoregulation in northern red-backed voles (myodes rutilus).perturbations in host energetics are considered to be an essential pathway for parasite impact on host fitness. however, direct estimations of parasite-induced variations in basal metabolic rates of vertebrate hosts have so far provided contradictory results. the energy requirements of immunity and other vital functions may be compromised in energy-demanding conditions in comparison to comfortable conditions; therefore, in our study performed on the wild red-backed vole, myodes rutilus, we compa ...201526341798
rickettsia raoultii in haemaphysalis erinacei from marbled polecats, china-kazakhstan border.we found rickettsia raoultii dna in 2 out of 32 (6.25 %) haemaphysalis erinacei ticks. result showed that the sequences of five genes (17-kda, glta, ompa, rrs, and ompb) were 100 % identity with that of r. raoultii in genbank. this study is the first report on the presence of r. raoultii in h. erinacei from wild marbled polecat, vormela peregusna. our findings suggest that h. erinacei parasitizing wild marbled polecat may serve as reservoir and carriers for r. raoultii in areas around the china- ...201526383238
molecular survey of anaplasma species in small ruminants reveals the presence of novel strains closely related to a. phagocytophilum in tunisia.a survey of anaplasma species in small ruminants is still lacking in north african countries. in this study, the presence of a. phagocytophilum, a. phagocytophilum-related species, and a. ovis was investigated in a total of 563 healthy small ruminants (303 goats and 260 sheep), from 25 randomly selected flocks sampled in tunisia. anaplasma spp. and a. ovis overall infection rates were 95.0% and 93.8% in sheep and 69.6% and 65.3% in goats, respectively. a. phagocytophilum was not detected in any ...201526394065
molecular detection of vector-borne agents in dogs from ten provinces of china.although many vector-borne agents are potential zoonoses and cause substantial morbidity and mortality in dogs worldwide, there are limited data on these organisms in dogs of china.201526428085
the diversity and prevalence of hard ticks attacking human hosts in eastern siberia (russian federation) with first description of invasion of non-endemic tick species.hard ticks are the vectors of many pathogens including tick-borne encephalitis virus and the lyme disease agent borrelia burgdorferi sensu lato. in eastern siberia, ixodes persulcatus, dermacentor nuttalli, dermacentor silvarum and haemaphysalis concinna are regarded as aggressive to humans. recently, significant changes in world tick fauna have been reported and this affects the spread of tick-borne pathogens. we studied the current species diversity, population structure and prevalence of tick ...201626443685
emerging tick-borne infections in mainland china: an increasing public health threat.since the beginning of the 1980s, 33 emerging tick-borne agents have been identified in mainland china, including eight species of spotted fever group rickettsiae, seven species in the family anaplasmataceae, six genospecies in the complex borrelia burgdorferi sensu lato, 11 species of babesia, and the virus causing severe fever with thrombocytopenia syndrome. in this review we have mapped the geographical distributions of human cases of infection. 15 of the 33 emerging tick-borne agents have be ...201526453241
natural hybridization between ixodes ricinus and ixodes persulcatus ticks evidenced by molecular genetics methods.the recently shown phenomenon of natural hybridization between ixodes persulcatus and ixodes pavlovskyi ticks (kovalev et al., 2015) stimulated similar studies in the sympatric zones of other tick species. in the present paper, 265 ixodes ricinus and i. persulcatus ticks from estonia were subjected to a search for interspecific hybrids based on nuclear (its2) and mitochondrial (cox1) markers as well as morphological features. surprisingly, only 72.1% of ticks morphologically identified as i. ric ...201626460161
identification and the preliminary in vitro characterization of iris homologue from salivary glands of ixodes persulcatus schulze.ixodes ricinus immunosuppressor (iris) is a tick salivary gland protein derived from i. ricinus. in this study, iris homolog was identified in the salivary glands of ixodes persulcatus, which is the specific vector of the lyme disease agent in japan. the homolog was named ipis-1. to investigate the function of ipis-1, we prepared a recombinant ipis-1 expressed in cos-7 cells as a rabbit igg fc-fused protein (ipis-1-ig). cell proliferation assay and ifn-γ elisa showed that ipis-1-ig inhibits the ...201626460162
prevalence and phylogenetic analysis of babesia spp. in ixodes ricinus and ixodes persulcatus ticks in latvia.babesia spp. are tick-borne protozoan parasites that have been reported in many european countries and are considered to be emerging pathogens. several babesia spp. have been identified in ticks in latvia. recently, canine babesiosis cases were diagnosed for the first time in latvia; therefore, continued studies on the prevalence and occurrence of new species are warranted. in the present study, questing tick samples collected in 2005-2007 were screened for the presence of babesia spp.; in total ...201626481239
serological survey of borrelia infection of dogs in sapporo, japan, where borrelia garinii infection was previously detected.a serological survey of borrelia infection of dogs was performed in sapporo, japan, where borrelia garinii infection in dogs was detected in 2011. a total of 314 serum samples were collected from dogs that visited three animal hospitals in sapporo from 2012 to 2014. the two-step evaluation method, involving screening elisa followed by western blot analysis, was used to detect antibodies against borrelia species. a total of 34 samples were positive by elisa. among those 34 samples, 32 were positi ...201526522809
ixodes scapularis and ixodes ricinus tick cell lines respond to infection with tick-borne encephalitis virus: transcriptomic and proteomic analysis.ixodid ticks are important vectors of a wide variety of viral, bacterial and protozoan pathogens of medical and veterinary importance. although several studies have elucidated tick responses to bacteria, little is known about the tick response to viruses. to gain insight into the response of tick cells to flavivirus infection, the transcriptomes and proteomes of two ixodes spp cell lines infected with the flavivirus tick-borne encephalitis virus (tbev) were analysed.201526582129
borrelia miyamotoi disease: neither lyme disease nor relapsing fever.borrelia miyamotoi disease (bmd) is a newly recognized borreliosis globally transmitted by ticks of the ixodes persulcatus species complex. once considered to be a tick symbiont with no public health implications, b miyamotoi is increasingly recognized as the agent of a nonspecific febrile illness often misdiagnosed as acute lyme disease without rash, or as ehrlichiosis. the frequency of its diagnosis in the northeastern united states is similar to that of human granulocytic ehrlichiosis. a diag ...201526593262
coinfection by ixodes tick-borne pathogens: ecological, epidemiological, and clinical consequences.ixodes ticks maintain a large and diverse array of human pathogens in the enzootic cycle, including borrelia burgdorferi and babesia microti. despite the poor ecological fitness of b. microti, babesiosis has recently emerged in areas endemic for lyme disease. studies in ticks, reservoir hosts, and humans indicate that coinfection with b. burgdorferi and b. microti is common, promotes transmission and emergence of b. microti in the enzootic cycle, and causes greater disease severity and duration ...201526613664
gis and remote sensing use in the exploration of lyme disease epidemiology.given the relatively recent recognition of lyme disease (ld) by cdc in 1990 as a nationally notifiable infectious condition, the rise of reported human cases every year argues for a better understanding of its geographic scope. the aim of this inquiry was to explore research conducted on spatiotemporal patterns of lyme disease in order to identify strategies for implementing vector and reservoir-targeted interventions. the focus of this review is on the use of gis-based methods to study populati ...201526633445
diversity of haemaphysalis-associated piroplasms of ruminants in central-eastern europe, hungary.increasing numbers of genetic variants are being recognized among piroplasms, but the precise taxonomical status, the tick vector and the geographical range of several species or genotypes are still unknown. bovine piroplasmosis was reported to re-emerge in north-east hungary. because theileria-infection was newly diagnosed in one cattle herd in the same region of the country, the aim of this study was to molecularly identify the relevant agent, to find its local vector tick species, and to exam ...201526652614
neurotropic virus infections as the cause of immediate and delayed neuropathology.a wide range of viruses from different virus families in different geographical areas, may cause immediate or delayed neuropathological changes and neurological manifestations in humans and animals. infection by neurotropic viruses as well as the resulting immune response can irreversibly disrupt the complex structural and functional architecture of the central nervous system, frequently leaving the patient or affected animal with a poor or fatal prognosis. mechanisms that govern neuropathogenes ...201526659576
[polymorphism in the phenotypic structure of a population of taiga tick and its epidemiological significance].the paper presents the results of 10-year (2005-2014) observations of an ixodespersulcatus schulze population. the purpose of this investigation was to trace long-term changes in the structure of the taiga tick population from the proportion of specimens with external skeletal anomalies and to assess a relationship between the pattern of imago phenotypic variation and the virus percentage of a carrier. there were a total of reports of the external skeletal structure of 1123 females gathered from ...201626720971
detection of babesia venatorum, anaplasma phagocytophilum and candidatus neoehrlichia mikurensis in ixodes persulcatus ticks from mongolia.information about the prevalence and geographical distribution of tick-borne pathogens anaplasma phagocytophilum, candidatus neoehrlichia mikurensis, and babesia spp. is still rare in mongolia. we tested 275 ixodes persulcatus ticks for a. phagocytophilum, cand. n. mikurensis and babesia spp. and 125 dermacentor nuttalli ticks especially for babesia spp. using different pcr methods. ticks were collected from three provinces (selenge, arkhangai, khentii) in mongolia. dna of a. phagocytophilum, ca ...201626739031
prevalence of borrelia miyamotoi in ixodes persulcatus in irkutsk city and its neighboring territories, russia.adult ixodes persulcatus were collected in highly populated districts in irkutsk city, russia, and in popular recreational and professional areas in its neighboring territories. borrelia miyamotoi infection in i. persulcatus was examined using multiplex taqman-pcr targeting 16s rdna, and nested pcr and sequencing analyses targeting flab and 16s rdna. b. miyamotoi and lyme disease borrelia species were detected in 13 (infection rate, 2.9%) and 77 (17.3%) out of 445 i. persulcatus ticks, respectiv ...201626750571
climate change, biodiversity, ticks and tick-borne diseases: the butterfly effect.we have killed wild animals for obtaining food and decimated forests for many reasons. nowadays, we are burning fossil fuels as never before and even exploring petroleum in deep waters. the impact of these activities on our planet is now visible to the naked eye and the debate on climate change is warming up in scientific meetings and becoming a priority on the agenda of both scientists and policy decision makers. on the occasion of the impact of environmental changes on infectious diseases (iec ...201526835253
[study on co-infection of tick-borne pathogens in ixodes persulcatus in charles hilary, xinjiang uygur autonomous region].to study the co-infection of tick-borne pathogens in ixodes persulcatus collected in charles hilary, xinjiang uygur autonomous region (xinjiang).201526837364
biosurveillance in central asia: successes and challenges of tick-borne disease research in kazakhstan and kyrgyzstan.central asia is a vast geographic region that includes five former soviet union republics: kazakhstan, kyrgyzstan, tajikistan, turkmenistan, and uzbekistan. the region has a unique infectious disease burden, and a history that includes silk road trade routes and networks that were part of the anti-plague and biowarfare programs in the former soviet union. post-soviet union biosurveillance research in this unique area of the world has met with several challenges, including lack of funding and res ...201626870722
functional structure and antimicrobial activity of persulcatusin, an antimicrobial peptide from the hard tick ixodes persulcatus.antimicrobial peptides (amps) are considered promising candidates for the development of novel anti-infective agents. in arthropods such as ticks, amps form the first line of defense against pathogens in the innate immune response. persulcatusin (ip) was found in the ixodes persulcatus midgut, and its amino acid sequence was reported. however, the complete structure of ip has not been identified. we evaluated the relation between structural features and antimicrobial activity of ip, and its pote ...201626873587
the role of particular tick developmental stages in the circulation of tick-borne pathogens affecting humans in central europe. 1. the general pattern.tick-borne pathogens are common in the natural environment, but their occurrence has a focal character. they occur in the natural environment in the form of the enzootic sources of infection. the general components include the animal reservoir, amplifiers and the efficient vector. however, the particular role of components can differ depending on the pathogen, the host range and possible transmission routes. animal reservoir of pathogen are vertebrate animals, being the hosts of pathogens. in eu ...201526878618
evaluation of different nested pcrs for detection of anaplasma phagocytophilum in ruminants and ticks.anaplasma phagocytophilum is a causative agent of granulocytic anaplasmosis in mammals, which has a broad geographical distribution and a high degree of clinical diversity. currently, numerous pcr assays have been developed and used for the detection of a. phagocytophilum in various specimens. however, their performance varies. the aim of this study was to evaluate the performance of five nested pcr assays by detection of 363 ruminant and tick samples, and to select the most appropriate methods ...201626911835
molecular epidemiological surveillance to assess emergence and re-emergence of tick-borne infections in tick samples from china evaluated by nested pcrs.an investigation was performed to detect eight pathogens in ticks collected from grass tips or animals in the southern, central and northeast regions of china. dna samples extracted from ticks were collected from ten different locations in eight provinces of china and subjected to screening for tick-borne pathogens, including borrelia burgdorferi sensu lato, ehrlichia spp., rickettsia spp., babesia/theileria spp., ehrlichia ruminantium, coxiella burnetii, and francisella tularensis, using nested ...201626943995
[ixodid ticks (parasitiformes: ixodidae) from small mammals in deforested boreal habitats in the northern european russia].changes in the population density of two hard tick species, ixodes (exopalpiger) trianguliceps birula, 1895 and ixodes persulcatus schulze, 1930, were examined in 1998-2001, and in 2003-2004 near gomselga village (kondopoga district, 62° 04' n, 33° 55' e) in central karelia. data on the abundance of ixodid ticks and the species composition of their hosts in 4 forest sites at different stages of post-felling regeneration (secondary succession), i. e. 7-14, 12-19, 25-32, and 80-87 after logging we ...201626946827
prevalence of anaplasma and bartonella spp. in ticks collected from korean water deer (hydropotes inermis argyropus).deer serve as reservoirs of tick-borne pathogens that impact on medical and veterinary health worldwide. in the republic of korea, the population of korean water deer (kwd, hydropotes inermis argyropus) has greatly increased from 1982 to 2011, in part, as a result of reforestation programs established following the korean war when much of the land was barren of trees. eighty seven haemaphysalis flava, 228 haemaphysalis longicornis, 8 ixodes nipponensis, and 40 ixodes persulcatus (21 larvae, 114 ...201626951985
trans-atlantic exchanges have shaped the population structure of the lyme disease agent borrelia burgdorferi sensu stricto.the origin and population structure of borrelia burgdorferi sensu stricto (s.s.), the agent of lyme disease, remain obscure. this tick-transmitted bacterial species occurs in both north america and europe. we sequenced 17 european isolates (representing the most frequently found sequence types in europe) and compared these with 17 north american strains. we show that trans-atlantic exchanges have occurred in the evolutionary history of this species and that a european origin of b. burgdorferi s. ...201626955886
genetic diversity and molecular characterization of babesia motasi-like in small ruminants and ixodid ticks from china.ovine babesioses, an important tick-borne disease of sheep and goats in china, is caused by the reproduction of intraerythrocytic protozoa of the babesia genus. babesia motasi-like is a babesia parasite that infects small ruminant in china, and two sub-groups of b. motasi-like can be subdivided based on differences in the rhoptry-associated-protein-1 gene. this study aimed to characterize the distribution, epidemiology and genetics of b. motasi-like in animals and ticks. a molecular investigatio ...201626976477
diversity of spotted fever group rickettsia infection in hard ticks from suifenhe, chinese-russian border.in order to investigate the diversity of spotted fever group (sfg) rickettsia infection in hard ticks, ticks were harvested from the forest areas in suifenhe city, along the chinese-russian border and conventional pcr was carried out using universal sfg rickettsia primers targeting glta and ompa genes to screen for their infection with sfg rickettsia organisms. results showed that of the 215 ticks belonging to ixodes persulcatus, haemaphysalis concinna and haemaphysalis japonica warburton, 1908 ...201626976703
life cycle of the taiga tick ixodes persulcatus (acari: ixodidae) in the north-west of russia.the life cycle of ixodes persulcatus lasts 3 years in the conditions of the leningrad province (north-west russia), the development of each phase taking a year. the normal age of the taiga tick is 3 years. the calendar age of larvae and nymphs reaches 11-12 months under favorable abiotic and biotic factors, while the calendar age of adults does not exceed 11 months. at the preimaginal phases of development the ticks that breed in august can feed before or after winter. however, their metamorphos ...201626979586
morphological features of ixodes persulcatus and i. ricinus hybrids: nymphs and adults.our aim was to reveal morphological features of first-generation ixodes persulcatus and i. ricinus hybrids (nymphs and adults) obtained under laboratory conditions for further study of natural populations of these species in sympatry foci. in 65 nymphs of three groups i. ricinus (23 specimens), i. persulcatus (21 specimens), and hybrids (21 specimens), 16 parameters were evaluated (length/width of the scutum and capitulum, length of the hypostome, palp, tarsus i, coxa i, sternal setae, and vario ...201626984610
tick-borne bacterial pathogens in southwestern finland.ixodes ricinus and ixodes persulcatus are the main vectors of lyme borreliosis spirochetes and several other zoonotic bacteria in northern europe and russia. however, few studies screening bacterial pathogens in finnish ticks have been conducted. therefore, reports on the occurrence and prevalence of several bacterial pathogens detected from ticks elsewhere in europe and russia are altogether missing from finland. the main aim of the current study was to produce novel data on the occurrence and ...201627004834
[effect of pyrethroids on taiga ticks (ixodes persulcatus ixodidae)].nonspecific prevention of infections, the agents of which are transmitted by ixodes ticks, is aimed at stopping the suction of the ticks to humans and is substantially based one the use of acaricides. the most interesting group of compounds to be used to individually protect humans is pyrethroids that cause different nerve conduction disturbances in the ticks, which result in their paralysis and death more significantly rapidly than the compounds from other chemical groups. the effect of 8 pyret ...201627029149
tick-borne infections in human and animal population worldwide.the abundance and activity of ectoparasites and its hosts are affected by various abiotic factors, such as climate and other organisms (predators, pathogens and competitors) presenting thus multiples forms of association (obligate to facultative, permanent to intermittent and superficial to subcutaneous) developed during long co-evolving processes. ticks are ectoparasites widespread globally and its eco epidemiology are closely related to the environmental conditions. they are obligatory hematop ...201527047089
genomic characteristics of chinese borrelia burgdorferi isolates.in china, b. burgdorferi, b.garinii, b. afzelii and b. yangtze sp. nov have been reported; b.garinii and b. afzelii are the main pathogenic genotypes. but until now only one chinese strain was reported with whole genome sequence. in order to further understand the genomic characteristics and diversity of chinese borrelia strains, 5 isolates from china were sequenced and compared with the whole genome sequences of strains in other areas. the results showed a high degree of conservation within the ...201627093540
tick repellents and acaricides of botanical origin: a green roadmap to control tick-borne diseases?arthropods are dangerous vectors of agents of deadly diseases, which may hit as epidemics or pandemics in the increasing world population of humans and animals. among them, ticks transmit more pathogen species than any other group of blood-feeding arthropods worldwide. thus, the effective and eco-friendly control of tick vectors in a constantly changing environment is a crucial challenge. a number of novel routes have been attempted to prevent and control tick-borne diseases, including the devel ...201627146901
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