| candidatus rickettsia andeanae, a spotted fever group agent infecting amblyomma parvum ticks in two brazilian biomes. | adult ticks of the species amblyomma parvum were collected from the vegetation in the pantanal biome (state of mato grosso do sul) and from horses in the cerrado biome (state of piauí) in brazil. the ticks were individually tested for rickettsial infection via polymerase chain reaction (pcr) targeting three rickettsial genes, glta, ompa and ompb. overall, 63.5% (40/63) and 66.7% (2/3) of a. parvum ticks from pantanal and cerrado, respectively, contained rickettsial dna, which were all confirmed ... | 0 | 24714968 |
| a systems biology approach to the characterization of stress response in dermacentor reticulatus tick unfed larvae. | dermacentor reticulatus (fabricius, 1794) is distributed in europe and asia where it infests and transmits disease-causing pathogens to humans, pets and other domestic and wild animals. however, despite its role as a vector of emerging or re-emerging diseases, very little information is available on the genome, transcriptome and proteome of d. reticulatus. tick larvae are the first developmental stage to infest hosts, acquire infection and transmit pathogens that are transovarially transmitted a ... | 2014 | 24586875 |
| rickettsia felis, an emerging flea-borne rickettsiosis. | rickettsia felis is an emerging insect-borne rickettsial pathogen and the causative agent of flea-borne spotted fever. first described as a human pathogen from the usa in 1991, r. felis is now identified throughout the world and considered a common cause of fever in africa. the cosmopolitan distribution of this pathogen is credited to the equally widespread occurrence of cat fleas (ctenocephalides felis), the primary vector and reservoir of r. felis. although r. felis is a relatively new member ... | 2016 | 27340613 |
| comparative evaluation of infected and noninfected amblyomma triste ticks with rickettsia parkeri, the agent of an emerging rickettsiosis in the new world. | the distribution of rickettsia parkeri in south america has been associated with amblyomma triste ticks. the present study evaluated under laboratory conditions two colonies of a. triste: one started from engorged females that were naturally infected by r. parkeri (designated as infected group); the other started from noninfected females (designated as control group). both colonies were reared in parallel for five consecutive generations. tick-naïve domestic rabbits were used for feeding of each ... | 2013 | 23936795 |
| rickettsia parkeri rickettsiosis, arizona, usa. | in the united states, all previously reported cases of rickettsia parkeri rickettsiosis have been linked to transmission by the gulf coast tick (amblyomma maculatum). here we describe 1 confirmed and 1 probable case of r. parkeri rickettsiosis acquired in a mountainous region of southern arizona, well beyond the recognized geographic range of a. maculatum ticks. the likely vector for these 2 infections was identified as the amblyomma triste tick, a neotropical species only recently recognized in ... | 0 | 27089251 |
| expanding range of amblyomma americanum and simultaneous changes in the epidemiology of spotted fever group rickettsiosis in the united states. | spotted fever group (sfg) rickettsia species are etiologic agents of a wide range of human infections from asymptomatic or mild infections to severe, life-threatening disease. in the united states, recent passive surveillance for sfg rickettsiosis shows an increased incidence and decreased severity of reported cases. the reasons for this are not well understood; however, we hypothesize that less pathogenic rickettsiae are causing more human infections, while the incidence of disease caused by mo ... | 2015 | 26503270 |
| discovery of filarial nematode dna in amblyomma americanum in northern virginia. | ticks collected in 2011 were screened for the presence of filarial nematode genetic material, and positive samples were sequenced for analysis. monanema-like filarial nematode dna was recently discovered in amblyomma americanum in northern virginia, marking the first time genetic material from this parasite has been discovered in ticks in the state. phylogenetic analysis revealed that this material was directly related to a previously discovered filarial nematode in a. americanum populations in ... | 2015 | 26707835 |
| correction: amblyomma maculatum feeding augments rickettsia parkeri infection in a rhesus macaque model: a pilot study. | | 2015 | 26322516 |
| the phenology of ticks and the effects of long-term prescribed burning on tick population dynamics in southwestern georgia and northwestern florida. | some tick populations have increased dramatically in the past several decades leading to an increase in the incidence and emergence of tick-borne diseases. management strategies that can effectively reduce tick populations while better understanding regional tick phenology is needed. one promising management strategy is prescribed burning. however, the efficacy of prescribed burning as a mechanism for tick control is unclear because past studies have provided conflicting data, likely due to a fa ... | 2014 | 25375797 |
| importation of exotic ticks and tick-borne spotted fever group rickettsiae into the united states by migrating songbirds. | birds are capable of carrying ticks and, consequently, tick-transmitted microorganisms over long distances and across geographical barriers such as oceans and deserts. ticks are hosts for several species of spotted fever group rickettsiae (sfgr), which can be transmitted to vertebrates during blood meals. in this study, the prevalence of this group of rickettsiae was examined in ticks infesting migratory songbirds by using polymerase chain reaction (pcr). during the 2009 and 2010 spring migratio ... | 2013 | 24252263 |
| rhipicephalus microplus serine protease inhibitor family: annotation, expression and functional characterisation assessment. | rhipicephalus (boophilus) microplus evades the host's haemostatic system through a complex protein array secreted into tick saliva. serine protease inhibitors (serpins) conform an important component of saliva which are represented by a large protease inhibitor family in ixodidae. these secreted and non-secreted inhibitors modulate diverse and essential proteases involved in different physiological processes. | 2015 | 25564202 |
| prevalence of rickettsiales in ticks removed from the skin of outdoor workers in north carolina. | tick-transmitted rickettsial diseases, such as ehrlichiosis and spotted fever rickettsiosis, are significant sources of morbidity and mortality in the southern united states. because of their exposure in tick-infested woodlands, outdoor workers experience an increased risk of infection with tick-borne pathogens. as part of a double blind randomized-controlled field trial of the effectiveness of permethrin-treated clothing in preventing tick bites, we identified tick species removed from the skin ... | 2014 | 25533148 |
| detection of rickettsia rickettsii, rickettsia parkeri, and rickettsia akari in skin biopsy specimens using a multiplex real-time polymerase chain reaction assay. | rickettsia rickettsii, rickettsia parkeri, and rickettsia akari are the most common causes of spotted fever group rickettsioses indigenous to the united states. infected patients characteristically present with a maculopapular rash, often accompanied by an inoculation eschar. skin biopsy specimens are often obtained from these lesions for diagnostic evaluation. however, a species-specific diagnosis is achieved infrequently from pathologic specimens because immunohistochemical stains do not diffe ... | 2014 | 24829214 |
| detection of rickettsia parkeri from within piura, peru, and the first reported presence of candidatus rickettsia andeanae in the tick rhipicephalus sanguineus. | domestic farm animals (n=145) were sampled for the presence of ectoparasites in northwestern peru during march, 2008. ninety domestic animals (62%) were positive for the presence of an ectoparasite(s) and produced a total collection of the following: 728 ticks [amblyomma maculatum, anocentor nitens, rhipicephalus (boophilus) microplus, rhipicephalus sanguineus, and otobius megnini], 12 lice (haematopinus suis), and 3 fleas (ctenocephalides felis). a rickettsia genus-specific qpcr assay was perfo ... | 2013 | 23488453 |
| spotted fever group rickettsiae in multiple hard tick species from fairfax county, virginia. | spotted fever group rickettsiosis (sfgr) is a potentially fatal disease that has displayed increasing incidence in the united states in recent years. the most well-known and severe type of this disease is rocky mountain spotted fever, but there are other mild forms that occur. recently, human infection with rickettsia parkeri has been reported and linked with the tick amblyomma maculatum. in 2010, a population of r. parkeri-infected a. maculatum was discovered in fairfax county, virginia, leadin ... | 2014 | 24978651 |
| cultivation of rickettsia amblyommii in tick cells, prevalence in florida lone star ticks (amblyomma americanum). | rickettsia amblyommii is a bacterium in the spotted fever group of organisms associated with the lone star tick (lst), amblyomma americanum. the lst is the most commonly reported tick to parasitize humans in the southeastern us. within this geographic region, there have been suspected cases of rocky mountain spotted fever (rmsf) where the causative agent, r. rickettsii, was not identified in the local tick population. in these areas, patients with clinical signs of rmsf had low or no detectable ... | 2014 | 24927809 |
| amblyomma maculatum feeding augments rickettsia parkeri infection in a rhesus macaque model: a pilot study. | rickettsia parkeri is an emerging eschar-causing human pathogen in the spotted fever group of rickettsia and is transmitted by the gulf coast tick, amblyomma maculatum. tick saliva has been shown to alter both the cellular and humoral components of the innate and adaptive immune systems. however, the effect of this immunomodulation on rickettsia transmission and pathology in an immunocompetent vertebrate host has not been fully examined. we hypothesize that, by modifying the host immune response ... | 2015 | 26244337 |
| immunoproteomic profiling of rickettsia parkeri and rickettsia amblyommii. | rickettsia parkeri is an amblyomma-associated, spotted fever group rickettsia species that causes an eschar-associated, febrile illness in multiple countries throughout the western hemisphere. many other rickettsial species of known or uncertain pathogenicity have been detected in amblyomma spp. ticks in the americas, including rickettsia amblyommii, "candidatus rickettsia andeanae" and rickettsia rickettsii. in this study, we utilized an immunoproteomic approach to compare antigenic profiles of ... | 2015 | 26234571 |
| frequency and distribution of rickettsiae, borreliae, and ehrlichiae detected in human-parasitizing ticks, texas, usa. | to describe the presence and distribution of tickborne bacteria and their vectors in texas, usa, we screened ticks collected from humans during 2008-2014 for rickettsia, borrelia, and ehrlichia spp. thirteen tick species were identified, and 23% of ticks carried bacterial dna from at least 1 of the 3 genera tested. | 0 | 26811941 |
| a francisella-like endosymbiont in the gulf coast tick evolved from a mammalian pathogen. | ticks (order ixodida) vector pathogenic bacteria that cause diseases in humans and other mammals. they also contain bacteria that are closely related to pathogens but function as endosymbionts that provide nutrients that are missing from mammalian blood-their sole food source. for instance, mammalian pathogens such as coxiella burnetii and francisella tularensis, as well as coxiella-like and francisella-like endosymbionts (cles and fles, respectively) occur in ticks worldwide. however, it is not ... | 2016 | 27645766 |
| rangelia vitalii in a free-ranging maned wolf (chrysocyon brachyurus) and co-infections. | an adult free-ranged female maned wolf was rescued from a periurban area subject to anthropogenic disturbances in the minas gerais, brazil. the animal presented poor body condition and anemia. the clinical condition rapidly deteriorated culminating in dead and a necropsy was performed. the main gross lesions were marked anemia and blood content in the intestines accompanied by many types of parasites. the protozoa rangelia vitalii was identified by histopathological analysis predominantly within ... | 2016 | 27761403 |
| the case for a 'one health' approach to combating vector-borne diseases. | | 2015 | 26027713 |
| high throughput techniques to reveal the molecular physiology and evolution of digestion in spiders. | spiders are known for their predatory efficiency and for their high capacity of digesting relatively large prey. they do this by combining both extracorporeal and intracellular digestion. whereas many high throughput ("-omics") techniques focus on biomolecules in spider venom, so far this approach has not yet been applied to investigate the protein composition of spider midgut diverticula (md) and digestive fluid (df). | 2016 | 27604083 |
| decreased small mammal and on-host tick abundance in association with invasive red imported fire ants (solenopsis invicta). | invasive species may impact pathogen transmission by altering the distributions and interactions among native vertebrate reservoir hosts and arthropod vectors. here, we examined the direct and indirect effects of the red imported fire ant (solenopsis invicta) on the native tick, small mammal and pathogen community in southeast texas. using a replicated large-scale field manipulation study, we show that small mammals were more abundant on treatment plots where s. invicta populations were experime ... | 0 | 27651533 |
| prediction of g protein-coupled receptor encoding sequences from the synganglion transcriptome of the cattle tick, rhipicephalus microplus. | the cattle tick, rhipicephalus (boophilus) microplus, is a pest which causes multiple health complications in cattle. the g protein-coupled receptor (gpcr) super-family presents a candidate target for developing novel tick control methods. however, gpcrs share limited sequence similarity among orthologous family members, and there is no reference genome available for r. microplus. this limits the effectiveness of alignment-dependent methods such as blast and pfam for identifying gpcrs from r. mi ... | 2016 | 26922323 |
| experimental infection of cotton rats and bobwhite quail with rickettsia parkeri. | amblyomma maculatum is the primary vector for rickettsia parkeri, a spotted fever group rickettsia (sfgr) and human pathogen. cotton rats and quail are known hosts for larval and nymphal a. maculatum; however, the role of these hosts in the ecology of r. parkeri is unknown. | 2013 | 23497681 |
| molecular characterization and functional significance of the vti family of snare proteins in tick salivary glands. | exocytosis involves membrane fusion between secretory vesicles and the plasma membrane. the soluble n-ethylmaleimide-sensitive factor attachment proteins (snaps) and their receptor proteins (snares) interact to fuse vesicles with the membrane and trigger the release of their sialosecretome out of the tick salivary gland cells. in this study, we examined the functional significance of the vti family of snare proteins of blood-feeding amblyomma maculatum and amblyomma americanum. vti1a and vti1b h ... | 2013 | 23499931 |
| effect of amblyomma maculatum (acari: ixodidae) saliva on the acute cutaneous immune response to rickettsia parkeri infection in a murine model. | rickettsia parkeri luckman (rickettsiales: rickettsiaceae) is a pathogenic spotted fever group rickettsia transmitted by amblyomma maculatum koch (acari: ixodidae) in the united states. the acute innate immune response to this pathogen and the effect of tick feeding or salivary components on this response is largely unknown. we hypothesized that a. maculatum saliva enhances r. parkeri infection via downregulation of the acute cellular and cytokine immune response. c3h/hen mice were intradermally ... | 2016 | 27521760 |
| comparative speed of kill of sarolaner (simparica™ chewables) and fluralaner (bravecto(®)) against induced infestations of amblyomma americanum on dogs. | the lone star tick, amblyomma americanum, infests dogs and cats in north america and transmits the pathogens ehrlichia chaffeensis and ehrlichia ewingii, which cause monocytic and granulocytic ehrlichiosis in dogs and humans, and cytauxzoon felis which causes cytauxzoonosis in cats. a parasiticide's speed of kill is important to minimize the direct deleterious effects [related to blood-feeding] of tick infestation and reduce the risk of transmission of tick-borne pathogens. in this study the spe ... | 2016 | 27430425 |
| deorphanization and target validation of cross-tick species conserved novel amblyomma americanum tick saliva protein. | we previously identified a cross-tick species conserved tick feeding stimuli responsive amblyomma americanum (aam) av422 gene. this study demonstrates that aamav422 belongs to a novel group of arthropod proteins that is characterized by 14 cysteine amino acid residues: c(23)-x7/9-c(33)-x23/24-c(58)-x8-c(67)-x7-c(75)-x23-c(99)-x15-c(115)-x10-c(126)-x24/25/33-c(150)c(151)-x7-c(159)-x8-c(168)-x23/24-c(192)-x9/10-c(202) predicted to form seven disulfide bonds. we show that aamav422 protein is a ubiq ... | 2013 | 23428900 |
| evasins: therapeutic potential of a new family of chemokine-binding proteins from ticks. | blood-sucking parasites, such as ticks, remain attached to their hosts for relatively long periods of time in order to obtain their blood meal without eliciting an immune response. one mechanism used to avoid rejection is the inhibition of the recruitment of immune cells, which can be achieved by a class of chemokine-binding proteins (ckbps) known as evasins. we have identified three distinct evasins produced by the salivary glands of the common brown dog tick, rhipicephalus sanguineus. they dis ... | 2016 | 27375615 |
| an overview of the components of aw-ipm campaigns against the new world screwworm. | the new world screwworm, cochliomyia hominivorax (coquerel), is one of the most damaging parasites of livestock, causing millions of dollars in annual losses to producers. the fly is an obligate parasite of warm-blooded animals, including humans. after a successful 50-year eradication campaign, c. hominivorax has been eradicated from the usa, mexico and central america by an area-wide integrated pest management approach. recently, caribbean and south american countries have expressed an interest ... | 2012 | 26466720 |
| rickettsia parkeri and candidatus rickettsia andeanae in gulf coast ticks, mississippi, usa. | | 0 | 23018026 |
| factors associated with tick bites and pathogen prevalence in ticks parasitizing humans in georgia, usa. | the incidence and emergence of tick-borne diseases has increased dramatically in the united states during the past 30 years, yet few large-scale epidemiological studies have been performed on individuals bitten by ticks. epidemiological information, including disease development, may provide valuable information regarding effectiveness of tick bite prevention education, pathogen transmission, human-disease dynamics, and potential implications for under reporting of tick-borne diseases. | 2016 | 26935205 |
| comparative speed of kill of oral treatments with simparica™ (sarolaner) and bravecto®(fluralaner) against induced infestations of rhipicephalus sanguineus on dogs. | rhipicephalus sanguineus is the most widely distributed tick species infesting dogs worldwide, which may cause discomfort to the host and transmit diseases. acaricides with a rapid and sustained speed of kill are thus important to prevent infestation and to reduce the risk of disease transmission. in this study, the speed of kill of a monthly administered simparica™ (sarolaner) treatment against induced infestations with r. sanguineus on dogs was evaluated and compared with a single dose of brav ... | 2016 | 26911244 |
| de novo transcriptome of the hemimetabolous german cockroach (blattella germanica). | the german cockroach, blattella germanica, is an important insect pest that transmits various pathogens mechanically and causes severe allergic diseases. this insect has long served as a model system for studies of insect biology, physiology and ecology. however, the lack of genome or transcriptome information heavily hinder our further understanding about the german cockroach in every aspect at a molecular level and on a genome-wide scale. to explore the transcriptome and identify unique sequen ... | 2014 | 25265537 |
| comparative speed of kill after treatment with simparica™ (sarolaner) and advantix®(imidacloprid + permethrin) against induced infestations of dermacentor reticulatus on dogs. | ticks are common ectoparasites that infest dogs globally. acaricides with rapid and sustained speed of kill are critical to control infestations and to reduce the risk of disease transmission. this study evaluated the speed of kill for 5 weeks after a single dose of orally administered simparica™ (sarolaner) against induced infestations with dermacentor reticulatus on dogs, compared to advantix® spot-on solution for dogs (imidacloprid + permethrin). | 2016 | 26911323 |
| deep sequencing analysis of the ixodes ricinus haemocytome. | ixodes ricinus is the main tick vector of the microbes that cause lyme disease and tick-borne encephalitis in europe. pathogens transmitted by ticks have to overcome innate immunity barriers present in tick tissues, including midgut, salivary glands epithelia and the hemocoel. molecularly, invertebrate immunity is initiated when pathogen recognition molecules trigger serum or cellular signalling cascades leading to the production of antimicrobials, pathogen opsonization and phagocytosis. we pres ... | 2015 | 25970599 |
| tissue- and time-dependent transcription in ixodes ricinus salivary glands and midguts when blood feeding on the vertebrate host. | ixodes ricinus is a tick that transmits the pathogens of lyme and several arboviral diseases. pathogens invade the tick midgut, disseminate through the hemolymph, and are transmitted to the vertebrate host via the salivary glands; subverting these processes could be used to interrupt pathogen transfer. here, we use massive de novo sequencing to characterize the transcriptional dynamics of the salivary and midgut tissues of nymphal and adult i. ricinus at various time points after attachment on t ... | 2015 | 25765539 |
| systematic revision of the adeleid haemogregarines, with creation of bartazoon n. g., reassignment of hepatozoon argantis garnham, 1954 to hemolivia, and molecular data on hemolivia stellata. | life cycles and molecular data for terrestrial haemogregarines are reviewed in this article. collection material was re-examined: hepatozoon argantis garnham, 1954 in argas brumpti was reassigned to hemolivia as hemolivia argantis (garnham, 1954) n. comb.; parasite dna was extracted from a tick crush on smear of an archived slide of hemolivia stellata in amblyomma rotondatum, then the 18s ssrrna gene was amplified by pcr. a systematic revision of the group is proposed, based on biological life c ... | 2015 | 26551414 |
| bioinformatic analyses of male and female amblyomma americanum tick expressed serine protease inhibitors (serpins). | serine protease inhibitors (serpins) are a diverse family of proteins that is conserved across taxa. the diversity of amblyomma americanum serpins (aas) is far more complex than previously thought as revealed by discovery of 57 and 33 aas transcripts that are respectively expressed in male and female a. americanum ticks, with 30 found in both. while distinct reproductively, both male and female metastriate ticks, such as a. americanum, require a blood meal. thus, 30 aas sequences found in both m ... | 2014 | 25238688 |
| tick-borne rickettsial pathogens in questing ticks, removed from humans and animals in mexico. | tick-borne rickettsial diseases (tbrd) are commonly encountered in medical and veterinary clinical settings. the control of these diseases is difficult, requiring disruption of a complex transmission chain involving a vertebrate host and ticks. the geographical distribution of the diseases is related to distribution of the vector, which is an indicator of risk for the population. a total of 1107 were collected by tick drag from forests, ecotourism parks and hosts at 101 sites in 22 of the 32 sta ... | 0 | 26726019 |
| dramatic expansion of the black widow toxin arsenal uncovered by multi-tissue transcriptomics and venom proteomics. | animal venoms attract enormous interest given their potential for pharmacological discovery and understanding the evolution of natural chemistries. next-generation transcriptomics and proteomics provide unparalleled, but underexploited, capabilities for venom characterization. we combined multi-tissue rna-seq with mass spectrometry and bioinformatic analyses to determine venom gland specific transcripts and venom proteins from the western black widow spider (latrodectus hesperus) and investigate ... | 2014 | 24916504 |
| ehrlichia and spotted fever group rickettsiae surveillance in amblyomma americanum in virginia through use of a novel six-plex real-time pcr assay. | the population of the lone star tick amblyomma americanum has expanded in north america over the last several decades. it is known to be an aggressive and nondiscriminatory biter and is by far the most common human-biting tick encountered in virginia. few studies of human pathogen prevalence in ticks have been conducted in our state since the mid-twentieth century. we developed a six-plex real-time pcr assay to detect three ehrlichia species (e. chaffeensis, e. ewingii, and panola mountain ehrli ... | 2014 | 24746145 |
| functional characterisation of a nicotinic acetylcholine receptor α subunit from the brown dog tick, rhipicephalus sanguineus. | ticks and tick-borne diseases have a major impact on human and animal health worldwide. current control strategies rely heavily on the use of chemical acaricides, most of which target the cns and with increasing resistance, new drugs are urgently needed. nicotinic acetylcholine receptors (nachrs) are targets of highly successful insecticides. we isolated a full-length nachr α subunit from a normalised cdna library from the synganglion (brain) of the brown dog tick, rhipicephalus sanguineus. phyl ... | 2013 | 24291321 |
| an insight into the microbiome of the amblyomma maculatum (acari: ixodidae). | the aim of this study was to survey the bacterial diversity of amblyomma maculatum koch, 1844, and characterize its infection with rickettsia parkeri. pyrosequencing of the bacterial 16s rrna was used to determine the total bacterial population in a. maculatum. pyrosequencing analysis identified rickettsia in a. maculatum midguts, salivary glands, and saliva, which indicates successful trafficking in the arthropod vector. the identity of rickettsia spp. was determined based on sequencing the ric ... | 0 | 24605461 |
| de novo characterization of the gene-rich transcriptomes of two color-polymorphic spiders, theridion grallator and t. californicum (araneae: theridiidae), with special reference to pigment genes. | a number of spider species within the family theridiidae exhibit a dramatic abdominal (opisthosomal) color polymorphism. the polymorphism is inherited in a broadly mendelian fashion and in some species consists of dozens of discrete morphs that are convergent across taxa and populations. few genomic resources exist for spiders. here, as a first necessary step towards identifying the genetic basis for this trait we present the near complete transcriptomes of two species: the hawaiian happy-face s ... | 2013 | 24314324 |
| a confirmed case of rickettsia parkeri infection in a traveler from uruguay. | the first confirmed case of rickettsia parkeri infection in uruguay is reported. to date, in south america, molecularly confirmed cases of human infection have been found in argentina and probably, brazil. our patient returned to spain after a 7-day trip to colonia suiza (southwestern uruguay). he presented fever (39°c), chills, and two eschars (tache noire-like) surrounded by an indurated, erythematous halo on the inner side of the left ankle besides a maculopapular rash on the legs. after trea ... | 2013 | 24166040 |
| molecular characterization of tick salivary gland glutaminyl cyclase. | glutaminyl cyclase (qc) catalyzes the cyclization of n-terminal glutamine residues into pyroglutamate. this post-translational modification extends the half-life of peptides and, in some cases, is essential in binding to their cognate receptor. due to its potential role in the post-translational modification of tick neuropeptides, we report the molecular, biochemical and physiological characterization of salivary gland qc during the prolonged blood feeding of the black-legged tick (ixodes scapul ... | 2013 | 23770496 |
| detecting rickettsia parkeri infection from eschar swab specimens. | the typical clinical presentation of several spotted fever group rickettsia infections includes eschars. clinical diagnosis of the condition is usually made by analysis of blood samples. we describe a more sensitive, noninvasive means of obtaining a sample for diagnosis by using an eschar swab specimen from patients infected with rickettsia parkeri. | 0 | 23647926 |
| redescription of hepatozoon felis (apicomplexa: hepatozoidae) based on phylogenetic analysis, tissue and blood form morphology, and possible transplacental transmission. | a hepatozoon parasite was initially reported from a cat in india in 1908 and named leucocytozoon felis domestici. although domestic feline hepatozoonosis has since been recorded from europe, africa, asia and america, its description, classification and pathogenesis have remained vague and the distinction between different species of hepatozoon infecting domestic and wild carnivores has been unclear. the aim of this study was to carry out a survey on domestic feline hepatozoonosis and characteriz ... | 2013 | 23587213 |
| rna interference-mediated depletion of n-ethylmaleimide sensitive fusion protein and synaptosomal associated protein of 25 kda results in the inhibition of blood feeding of the gulf coast tick, amblyomma maculatum. | the signalling pathways in tick salivary glands that control 'sialo-secretome' secretion at the tick-host interface remain elusive; however, this complex process is essential for successful feeding and manipulation of the host haemostatic response. exocytosis of the sialo-secretome in the salivary glands requires a core of soluble n-ethylmaleimide-sensitive fusion (nsf) attachment proteins (snaps) and receptor proteins (snares). snares have been identified as the key components in regulating the ... | 2013 | 23437815 |
| targeting of insect epicuticular lipids by the entomopathogenic fungus beauveria bassiana: hydrocarbon oxidation within the context of a host-pathogen interaction. | broad host range entomopathogenic fungi such as beauveria bassiana attack insect hosts via attachment to cuticular substrata and the production of enzymes for the degradation and penetration of insect cuticle. the outermost epicuticular layer consists of a complex mixture of non-polar lipids including hydrocarbons, fatty acids, and wax esters. long chain hydrocarbons are major components of the outer waxy layer of diverse insect species, where they serve to protect against desiccation and microb ... | 2013 | 23422735 |
| evidence of antibodies to spotted fever group rickettsiae in small mammals and quail from mississippi. | rickettsia parkeri is a recently recognized human pathogen primarily associated with the gulf coast tick amblyomma maculatum, with immature stages of this tick reported from wild vertebrates. to better understand the role of vertebrates in the natural history of this bacterium, we evaluated small mammals and ground-dwelling birds for evidence of infection with r. parkeri or exposure to the organism. we sampled small mammals (n=39) and passerines (n=47) in both north-central and southeast mississ ... | 2012 | 23199266 |
| rubber elongation factor (ref), a major allergen component in hevea brasiliensis latex has amyloid properties. | ref (hevb1) and srpp (hevb3) are two major components of hevea brasiliensis latex, well known for their allergenic properties. they are obviously taking part in the biosynthesis of natural rubber, but their exact function is still unclear. they could be involved in defense/stress mechanisms after tapping or directly acting on the isoprenoid biosynthetic pathway. the structure of these two proteins is still not described. in this work, it was discovered that ref has amyloid properties, contrary t ... | 2012 | 23133547 |
| "scanning mutagenesis" of the amino acid sequences flanking phosphorylation site 1 of the mitochondrial pyruvate dehydrogenase complex. | the mitochondrial pyruvate dehydrogenase complex (mtpdc) is regulated by reversible seryl-phosphorylation of the e1α subunit by a dedicated, intrinsic kinase. the phospho-complex is reactivated when dephosphorylated by an intrinsic pp2c-type protein phosphatase. both the position of the phosphorylated ser-residue and the sequences of the flanking amino acids are highly conserved. we have used the synthetic peptide-based kinase client (kic) assay plus recombinant pyruvate dehydrogenase e1α and e1 ... | 2012 | 22811682 |
| efficacy of an imidacloprid/flumethrin collar against fleas, ticks, mites and lice on dogs. | the studies reported here were conducted to ascertain the efficacy of imidacloprid/flumethrin incorporated in a slow-release matrix collar, against infestations of dogs by fleas, ticks, mites and lice. efficacy was evaluated against the flea ctenocephalides felis felis, the ticks rhipicephalus sanguineus, ixodes ricinus, ixodes scapularis, dermacentor reticulatus and dermacentor variabilis, the mite sarcoptes scabiei and the biting louse trichodectes canis. | 2012 | 22647530 |
| crystallization and preliminary crystallographic characterization of the n-terminal kunitz domain of boophilin. | boophilin is a tight-binding thrombin inhibitor composed of two canonical kunitz-type domains in a tandem arrangement. thrombin-bound boophilin can inhibit a second trypsin-like serine proteinase, most likely through the reactive loop of its n-terminal kunitz domain. here, the crystallization and preliminary crystallographic analysis of the isolated n-terminal domain of boophilin is reported. the crystals belonged to the orthorhombic space group p2(1)2(1)2(1) and diffracted to beyond 1.8 å resol ... | 2012 | 22505414 |
| cyp52x1, representing new cytochrome p450 subfamily, displays fatty acid hydroxylase activity and contributes to virulence and growth on insect cuticular substrates in entomopathogenic fungus beauveria bassiana. | infection of insects by the entomopathogenic fungus beauveria bassiana proceeds via attachment and penetration of the host cuticle. the outermost epicuticular layer or waxy layer of the insect represents a structure rich in lipids including abundant amounts of hydrocarbons and fatty acids. a member of a novel cytochrome p450 subfamily, cyp52x1, implicated in fatty acid assimilation by b. bassiana was characterized. b. bassiana targeted gene knockouts lacking bbcyp52x1 displayed reduced virulence ... | 2012 | 22393051 |
| the legacy of dr. ralph seal paffenbarger, jr. - past, present, and future contributions to physical activity research. | | 0 | 21253445 |
| survey of ticks collected from tennessee cattle and their pastures for anaplasma and ehrlichia species. | anaplasma marginale is the causative agent for bovine anaplasmosis (ba) and ehrlichia ruminantium is the causative agent for heartwater, 2 devastating diseases of cattle. ba is common in the united states and frequently reported in western tennessee cattle; however, cases of heartwater are not yet established in the continental united states. because both pathogens are transmitted via the bites of infected ticks, the objective of this study was to survey cattle and pastures for ticks and for eac ... | 2016 | 26348980 |
| molecular characterization of anaplasma marginale in ticks naturally feeding on buffaloes. | anaplasma marginale is the most prevalent pathogen transmitted by ticks in cattle in tropical and subtropical regions of the world. however, the tick species involved in the transmission of a. marginale in buffaloes in brazil have not been identified. the objective of the present study was to determine the presence of a. marginale in ticks parasitizing water buffaloes. a total of 200 samples of rhipicephalus microplus, dermacentor nitens, amblyomma cajennense, and amblyomma maculatum were collec ... | 2015 | 26209411 |
| evaluation of gulf coast ticks (acari: ixodidae) for ehrlichia and anaplasma species. | amblyomma maculatum koch (the gulf coast tick) is an aggressive, human-biting ixodid tick distributed throughout much of the southeastern united states and is the primary vector for rickettsia parkeri, an emerging human pathogen. amblyomma maculatum has diverse host preferences that include white-tailed deer, a known reservoir for ehrlichia and anaplasma species, including the human pathogens e. ewingii and e. chaffeensis to examine more closely the potential role of a. maculatum in the maintena ... | 2016 | 28031351 |
| seroepidemiologic study of human infections with spotted fever group rickettsiae in north carolina. | increasing entomologic and epidemiologic evidence suggests that spotted fever group rickettsiae (sfgr) other than rickettsia rickettsii are responsible for spotted fever rickettsioses in the united states. a retrospective seroepidemiologic study was conducted on stored acute- and convalescent-phase sera that had been submitted for rocky mountain spotted fever testing to the north carolina state laboratory of public health. we evaluated the serologic reactivity of the paired sera to r. rickettsii ... | 2014 | 25187639 |
| rickettsia parkeri and rickettsia montanensis, kentucky and tennessee, usa. | we found that 14.3% (15/105) of amblyomma maculatum and 3.3% (10/299) of dermacentor variabilis ticks collected at 3 high-use military training sites in west-central kentucky and northern tennessee, usa, were infected with rickettsia parkeri and rickettsia montanensis, respectively. these findings warrant regional increased public health awareness for rickettsial pathogens and disease. | 0 | 25271771 |
| rickettsial disease in the peruvian amazon basin. | using a large, passive, clinic-based surveillance program in iquitos, peru, we characterized the prevalence of rickettsial infections among undifferentiated febrile cases and obtained evidence of pathogen transmission in potential domestic reservoir contacts and their ectoparasites. blood specimens from humans and animals were assayed for spotted fever group rickettsiae (sfgr) and typhus group rickettsiae (tgr) by elisa and/or pcr; ectoparasites were screened by pcr. logistic regression was used ... | 2016 | 27416029 |
| diversity of piroplasms detected in blood-fed and questing ticks from several states in the united states. | piroplasms in the genera babesia, theileria, and cytauxzoon are tick-borne parasites that may be animal and human pathogens. most piroplasms with known life cycles are transmitted by ixodid ticks; however, for many species, the vector is unknown. this study was conducted to determine the prevalence and diversity of piroplasms in ticks from several us states. piroplasm-specific polymerase chain reaction (pcr) assays were used to test 1631 ticks from georgia (n=486), kentucky (n=103), pennsylvania ... | 2014 | 24709338 |
| the evolving medical and veterinary importance of the gulf coast tick (acari: ixodidae). | amblyomma maculatum koch (the gulf coast tick) is a three-host, ixodid tick that is distributed throughout much of the southeastern and south-central united states, as well as several countries throughout central and south america. a considerable amount of scientific literature followed the original description of a. maculatum in 1844; nonetheless, the gulf coast tick was not recognized as a vector of any known pathogen of animals or humans for >150 years. it is now identified as the principal v ... | 2015 | 26336308 |
| bacteria of the genus rickettsia in ticks (acari: ixodidae) collected from birds in costa rica. | the aim of this study was to document the presence of rickettsia spp. in ticks parasitizing wild birds in costa rica. birds were trapped at seven locations in costa rica during 2004, 2009, and 2010; then visually examined for the presence of ticks. ticks were identified, and part of them was tested individually for the presence of rickettsia spp. by polymerase chain reaction (pcr) using primers targeting fragments of the rickettsial genes glta and ompa. pcr products were dna-sequenced and analyz ... | 2015 | 25869035 |
| molecular detection of rickettsia species within ticks (acari: ixodidae) collected from arkansas united states. | rocky mountain spotted fever (rmsf), caused by the etiological agent rickettsia rickettsii, is the most severe and frequently reported rickettsial illness in the united states, and is commonly diagnosed throughout the southeast. with the discoveries of rickettsia parkeri and other spotted fever group rickettsiae (sfgr) in ticks, it remains inconclusive if the cases reported as rmsf are truly caused by r. rickettsii or other sfgr. arkansas reports one of the highest incidence rates of rmsf in the ... | 2015 | 26334827 |
| catalase is a determinant of the colonization and transovarial transmission of rickettsia parkeri in the gulf coast tick amblyomma maculatum. | the gulf coast tick (amblyomma maculatum) has evolved as a competent vector of the spotted-fever group rickettsia, rickettsia parkeri. in this study, the functional role of catalase, an enzyme responsible for the degradation of toxic hydrogen peroxide, in the colonization of the tick vector by r. parkeri and transovarial transmission of this pathogen to the next tick generation, was investigated. catalase gene (cat) expression in midgut, salivary glands and ovarian tissues exhibited a 2-11-fold ... | 2017 | 28370634 |
| rickettsia parkeri and "candidatus rickettsia andeanae" in questing amblyomma maculatum (acari: ixodidae) from mississippi. | amblyomma maculatum koch (acari: ixodidae), the primary vector for rickettsia parkeri, may also be infected with a rickettsia of unknown pathogenicity, "candidatus rickettsia andeanae." infection rates with these rickettsiae vary geographically, and coinfected ticks have been reported. in this study, infection rates of r. parkeri and "ca r. andeanae" were evaluated, and rickettsial dna levels quantified, in 335 questing adult a. maculatum collected in 2013 (n = 95), 2014 (n = 139), and 2015 (n = ... | 2016 | 27773866 |
| [amblyomma triste and amblyomma tigrinum (acari: ixodidae) in sympatry in santa fe province, argentina]. | the aim of this communication is to report, for the first time, the occurrence of amblyomma triste in santa fe province, argentina, and to add a new isolation place for amblyomma tigrinum. both species of ticks are vectors of rickettsia parkeri, a spotted fever group rickettsia. ticks were recovered from tourists in august 2014 and december 2015 at the federico wildermuth foundation (31° 59's, 61° 24'o), san martin department, santa fe province. five adult ticks were morphologically identified a ... | 2016 | 27723618 |
| evaluation of human attachment by larval amblyomma maculatum (acari: ixodidae). | the tick, amblyomma maculatum koch (gulf coast tick), has recently been shown to be an important disease vector of both medical and veterinary concern. although much is known about the behavior and ecology of adults, little is known of the immatures. larval feeding on humans has never been demonstrated (and thus, there are no collection records from humans). in this experiment, 10 larval a. maculatum, amblyomma americanum (l.) (a positive control), and dermacentor variabilis (say) (a negative co ... | 2016 | 26576936 |
| rickettsia parkeri transmission to amblyomma americanum by cofeeding with amblyomma maculatum (acari: ixodidae) and potential for spillover. | amblyomma americanum (l.) is a human-biting ixodid tick distributed throughout much of the southeastern united states. rickettsia parkeri is a member of the spotted fever group rickettsiae and causes a febrile illness in humans commonly referred to as "tidewater spotted fever" or "r. parkeri rickettsiosis." although the gulf coast tick, amblyomma maculatum koch, is the primary vector of r. parkeri, a small proportion of a. americanum have also been shown to harbor r. parkeri. the purpose of this ... | 2015 | 26336226 |
| ultrastructure of presumed "candidatus rickettsia andeanae" in amblyomma maculatum (acari: ixodidae). | amblyomma maculatum koch, 1844 (also known as the gulf coast tick) is found in parts of the americas, including the central and southern united states. its primary importance is as the vector of rickettsia parkeri, a spotted fever group rickettsia that causes an illness similar to, but milder than, rocky mountain spotted fever. a second spotted fever group rickettsia, "candidatus rickettsia andeanae," was detected in gulf coast ticks approximately 10 yr ago. however, the significance of this org ... | 2014 | 26309324 |
| experimental vertical transmission of rickettsia parkeri in the gulf coast tick, amblyomma maculatum. | rickettsia parkeri, an obligate intracellular bacterium, is a member of the spotted fever group of rickettsiae (sfgr), and is transmitted to humans and other animals by invertebrate vectors. in the united states, the primary vector of r. parkeri is the gulf coast tick, amblyomma maculatum koch. this study investigates the vertical transmission dynamics of r. parkeri within a field-derived, naturally infected colony of a. maculatum. transovarial and transstadial transmission of the pathogen was o ... | 2015 | 25958197 |
| gulf coast tick rash illness in mississippi caused by rickettsia parkeri. | | 2014 | 25252425 |
| in vitro isolation and infection intensity of rickettsia parkeri in amblyomma triste ticks from the paraná river delta region, argentina. | in the present study, we report the first in vitro isolation and infection intensity of rickettsia parkeri in amblyomma triste ticks from argentina. no genetic differences in the molecular targets evaluated were found between r. parkeri isolates from argentina and those r. parkeri isolates reported in uruguay and brazil, both obtained from a. triste. only a minor difference was observed when compared to r. parkeri isolated from amblyomma maculatum from united states. moreover, the prevalence of ... | 2014 | 25113981 |
| isolation of "candidatus rickettsia andeanae" (rickettsiales: rickettsiaceae) in embryonic cells of naturally infected amblyomma maculatum (ixodida: ixodidae). | the gulf coast tick, amblyomma maculatum koch, has become increasingly important in public health for its role as a vector of the recently recognized human pathogen, rickettsia parkeri. more recently, these ticks were also found to harbor a novel spotted fever group rickettsia, "candidatus rickettsia andeanae." first identified in peru, and subsequently reported in ticks collected in the united states, chile, and argentina, "ca. r. andeanae" remains largely uncharacterized, in part because of th ... | 2013 | 24180118 |
| population analyses of amblyomma maculatum ticks and rickettsia parkeri using single-strand conformation polymorphism. | gulf coast ticks, amblyomma maculatum, and the zoonotic agents they transmit, rickettsia parkeri, are expanding into areas in the united states where they were not previously reported, and are emerging threats for public and veterinary health. the dynamics of this tick-pathogen system and implications for disease transmission are still unclear. to assess genetic variation of tick and rickettsial populations, we collected adult a. maculatum from 10 sites in mississippi, 4 in the northern, one in ... | 2013 | 23856472 |
| action on the surface: entomopathogenic fungi versus the insect cuticle. | infections mediated by broad host range entomopathogenic fungi represent seminal observations that led to one of the first germ theories of disease and are a classic example of a co-evolutionary arms race between a pathogen and target hosts. these fungi are able to parasitize susceptible hosts via direct penetration of the cuticle with the initial and potentially determining interaction occurring between the fungal spore and the insect epicuticle. entomogenous fungi have evolved mechanisms for a ... | 2013 | 26462424 |
| update on tick-borne rickettsioses around the world: a geographic approach. | tick-borne rickettsioses are caused by obligate intracellular bacteria belonging to the spotted fever group of the genus rickettsia. these zoonoses are among the oldest known vector-borne diseases. however, in the past 25 years, the scope and importance of the recognized tick-associated rickettsial pathogens have increased dramatically, making this complex of diseases an ideal paradigm for the understanding of emerging and reemerging infections. several species of tick-borne rickettsiae that wer ... | 0 | 24092850 |
| prevalence of antibodies to spotted fever group rickettsia spp. and ehrlichia spp. in coyotes (canis latrans) in oklahoma and texas, usa. | coyotes (canis latrans) are commonly infested with ticks, including amblyomma americanum, the predominant vector of ehrlichia chaffeensis and ehrlichia ewingii; dermacentor variabilis, an important vector of rickettsia rickettsii; and amblyomma maculatum, a major vector of rickettsia parkeri, a spotted fever group (sfg) rickettsia. to determine the degree to which coyotes are infected with or exposed to tick-borne bacterial disease agents, serum samples collected from coyotes in oklahoma and tex ... | 2013 | 23778619 |
| prevalence of rickettsia species in dermacentor variabilis ticks from ontario, canada. | relatively little is known about the prevalence of rickettsial species in dermacentor ticks in eastern canada. in this study, dermacentor ticks from the province of ontario, canada, were tested for the presence of spotted fever group rickettsial (sfgr) species, coxiella burnetii and francisella tularensis. rickettsia rickettsii was not detected in any ticks tested, but r. montanensis was detected at a prevalence of 2.2% in d. variabilis (17/778). two other sfgr species, r. parkeri and candidatus ... | 2016 | 27318438 |
| multiplex qpcr assay for identification and differentiation of amblyomma americanum, amblyomma cajennense, and amblyomma maculatum (ixodida: ixodidae) tick species in the eastern united states. | many ticks of the genus amblyomma are vectors of human pathogens, and the correct species identification is medically and epidemiologically important. morphological identification is time-consuming and requires a high level of expertise. identification of engorged, immature, or damaged ticks and the differentiation of closely related species remain problematic. here, we report the development of a real-time taqman assay for the genomic identification and differentiation of amblyomma americanum ( ... | 2014 | 25118411 |
| occurrence and county-level distribution of ticks (acari: ixodoidea) in nebraska using passive surveillance. | a 100 yr (1911-2011) examination of tick submissions was compiled from the u.s. national tick collection and three state databases to determine tick species occurrence in nebraska sixteen tick species were identified including amblyomma americanum (l.), dermacentor variabilis (say), dermacentor albipictus (packard), dermacentor andersoni stiles, haemaphysalis leporispalustris (packard), rhipicephalus sanguineus (latreille), and otobius megrini (dughs). amblyomma maculatum koch and ixodes scapula ... | 2014 | 24724283 |
| determination of the effective dose of a novel oral formulation of sarolaner (simparica™) for the treatment and month-long control of fleas and ticks on dogs. | three laboratory studies were conducted to determine the appropriate dose of sarolaner, a novel isoxazoline, for the treatment and month-long control of infestations of fleas and ticks on dogs. in the first study, dogs were treated orally with sarolaner suspension formulations at 1.25, 2.5 or 5.0mg/kg, and infested with dermacentor reticulatus, rhipicephalus sanguineus ticks and with ctenocephalides felis felis (cat flea) prior to treatment and then weekly for up to 8 weeks. fleas and ticks were ... | 2016 | 26948830 |
| emerging tick-borne rickettsia and ehrlichia at joint base langley-eustis, fort eustis, virginia. | four species of ticks known to parasitize humans (amblyomma americanum (lone star tick), dermacentor variabilis (american dog tick), amblyomma maculatum (gulf coast tick), and ixodes scapularis (black-legged tick)) were collected at joint base langley-eustis, fort eustis, virginia during 2009. these ticks were tested individually (adults and nymphs) and in pools of 15 (larvae) for pathogens of public health importance within the genera: rickettsia, borrelia, and ehrlichia, by quantitative real-t ... | 2016 | 27613206 |
| the effects of solenopsis invicta (hymenoptera: formicidae) and burned habitat on the survival of amblyomma americanum (acari: ixodidae) and amblyomma maculatum (acari: ixodidae). | identifying ways in which humans can reduce tick populations is important for preventing the spread and emergence of diseases. during a recent study on effects of long-term prescribed burning on ticks, differences in species composition were observed with lone star ticks, amblyomma americanum (l.), preferring unburned habitats and gulf coast ticks, amblyomma maculatum (koch), preferring burned habitats. interestingly, the red imported fire ant, solenopsis invicta buren, is found predominantly in ... | 2013 | 23540113 |
| evaluation of the speed of kill of sarolaner (simparica™) against induced infestations of three species of ticks (amblyomma maculatum, ixodes scapularis, ixodes ricinus) on dogs. | the rapid speed of kill of sarolaner (simparica™, zoetis), a novel isoxazoline compound, was demonstrated against three tick species known to infest dogs in europe or the united states. efficacy was measured against an existing infestation and against subsequent weekly re-infestations for 35 days after treatment. dogs were randomly allocated to treatment with a single oral dose of either placebo or sarolaner (2mg/kg) based on pre-treatment host-suitability tick counts. dogs were infested with ap ... | 2016 | 26928659 |
| assessment of tick antioxidant responses to exogenous oxidative stressors and insight into the role of catalase in the reproductive fitness of the gulf coast tick, amblyomma maculatum. | as obligate blood-sucking ectoparasites, to avoid tissue damage, ticks must neutralize the reactive oxygen species (ros) generated from uptake and digestion of a bloodmeal. consequently, ticks utilize a battery of antioxidant molecules, including catalase (cat), an enzyme that converts hydrogen peroxide (h2 o2 ) into water and oxygen. here, we investigated the tick antioxidant machinery by exogenous injection of sublethal doses of h2 o2 or paraquat. the relative transcript levels of selected amb ... | 2016 | 26919203 |
| field observations of questing and dispersal by colonized nymphal amblyomma maculatum koch (acari: ixodidae). | : almost nothing is known about the questing and dispersal behavior of immature gulf coast ticks (amblyomma maculatum), a vector of both medical and veterinary concern. this experiment examined host-seeking (questing) and dispersal of marked, previously colonized, nymphal a. maculatum released in field plots in rural oktibbeha county, mississippi during 2015. a total of 500 (250 per replication) a. maculatum nymphs were painted and released in 5 plots (50 ticks each time). observations were the ... | 2016 | 26824469 |
| structural insights into divalent cation modulations of atp-gated p2x receptor channels. | p2x receptors are trimeric atp-gated cation channels involved in physiological processes ranging widely from neurotransmission to pain and taste signal transduction. the modulation of the channel gating, including that by divalent cations, contributes to these diverse physiological functions of p2x receptors. here, we report the crystal structure of an invertebrate p2x receptor from the gulf coast tick amblyomma maculatum in the presence of atp and zn(2+) ion, together with electrophysiological ... | 2016 | 26804916 |
| genomics, phylogeny and in silico analysis of mitochondrial glutathione s-transferase-kappa from the camel camelus dromedarius. | the domesticated one-humped camel, camelus dromedarius, is one of the most important animals in the arabian peninsula. for most of its life, this species is exposed to both intrinsic and extrinsic genotoxic factors that cause gross dna alterations in many organisms. gst enzymes constitute an important supergene family involved in protection against the deleterious effects of oxidative stress and xenobiotics. cloning the camel mitochondrial gst kappa (gstk) gene and comparing its structural simil ... | 2014 | 24810173 |
| a third amblyomma species and the first tick-borne rickettsia in chile. | during november 2010, three ticks were collected from three dogs living in the rural area of arica, northern chile. morphological analyses of the ticks in the laboratory revealed that they were most similar to amblyomma maculatum koch and amblyomma triste koch. however, because of unique metatarsal spurs, neither of the chilean specimens could be assigned with certainty to a. maculatum or a. triste, based on external morphology. the mitochondrial 16s rrna gene partial sequences obtained from two ... | 2012 | 22308792 |
| investigating the adult ixodid tick populations and their associated anaplasma, ehrlichia, and rickettsia bacteria at a rocky mountain spotted fever hotspot in western tennessee. | ehrlichiosis and rickettsiosis are two common bacterial tick-borne diseases in the southeastern united states. ehrlichiosis is caused by ehrlichiae transmitted by amblyomma americanum and rickettsiosis is caused by rickettsiae transmitted by amblyomma maculatum and dermacentor variabilis. these ticks are common and have overlapping distributions in the region. the objective of this study was to identify anaplasma, ehrlichia, and rickettsia species associated with questing ticks in a rocky mounta ... | 2017 | 28598270 |
| comparative vertical transmission of rickettsia by dermacentor variabilis and amblyomma maculatum. | the geographical overlap of multiple rickettsia and tick species coincides with the molecular detection of a variety of rickettsial agents in what may be novel tick hosts. however, little is known concerning transmissibility of rickettsial species by various tick hosts. to examine the vertical transmission potential between select tick and rickettsial species, two sympatric species of ticks, dermacentor variabilis and amblyomma maculatum, were exposed to five different rickettsial species, inclu ... | 2017 | 28433729 |
| efficacy of a novel oral formulation of sarolaner (simparica™) against five common tick species infesting dogs in the united states. | the efficacy of a single oral treatment with sarolaner (simparica™, zoetis), a novel isoxazoline compound, was evaluated against five tick species known to infest dogs in the united states. a total of 10 laboratory studies, two against each species, were conducted using adult purpose-bred mongrels or beagle dogs. in each study, 16 dogs were randomly allocated to one of two treatment groups based on pre-treatment host-suitability tick counts. dogs were infested with approximately 50 unfed adult a ... | 2016 | 26935819 |
| comparison of novel and conventional methods of trapping ixodid ticks in the southeastern u.s.a. | tick-borne disease surveillance and research rely on resource-effective methods for tick collection. this study compared the respective performances of several trapping methods in a mixed grassland-forest habitat in western tennessee. to test for temporal differences in effectiveness, sites were sampled monthly (april-august 2013) using dry ice, dragging, flagging, sweep netting, carbon dioxide (co2 ) dragging and co2 flagging methods. to evaluate the effect of habitat on method effectiveness, f ... | 2016 | 26801319 |