| interstitial pulmonary inflammation due to microbacterium sp. after heart transplantation. | a coryneform bacterium was isolated from the bronchoalveolar aspirate of a patient with interstitial pulmonary inflammation. commercial systems identified the isolate as corynebacterium sp. or aureobacterium sp./corynebacterium aquaticum, but 16s rrna gene analysis unequivocally attributed it to the genus microbacterium. this represents the first documented case of microbacterium pulmonary infection. | 2006 | 16476799 |
| identifying the bacterial community on the surface of intralox belting in a meat boning room by culture-dependent and culture-independent 16s rdna sequence analysis. | we examined the bacterial community present on an intralox conveyor belt system in an operating lamb boning room by sequencing the 16s ribosomal dna (rdna) of bacteria extracted in the presence or absence of cultivation. rflp patterns for 16s rdna clone library and cultures were generated using haeiii and mspi restriction endonucleases. 16s rdna amplicons produced 8 distinct rflp pattern groups. rflp groups i-iv were represented in the clone library and rflp groups i and v-viii were represented ... | 2006 | 16488497 |
| microbacterium aerolatum sp. nov., isolated from the air in the 'virgilkapelle' in vienna. | three rod-shaped, gram-positive strains were isolated from the air of the chapel 'virgilkapelle' in vienna. a representative of these three strains, strain v-73t, shared the highest 16s rdna sequence similarities with members of the genus microbacterium, in particular microbacterium foliorum, microbacterium testaceum, microbacterium esteraromaticum, microbacterium keratanolyticum and microbacterium arabinogalactanolyticum. the strains displayed almost identical biochemical and physiological char ... | 2002 | 12148633 |
| the microbacteria ii. : the growth of microbacterium lacticum in a chemically defined medium. | | 1949 | 16561650 |
| planktonic replication is essential for biofilm formation by legionella pneumophila in a complex medium under static and dynamic flow conditions. | legionella pneumophila persists for a long time in aquatic habitats, where the bacteria associate with biofilms and replicate within protozoan predators. while l. pneumophila serves as a paradigm for intracellular growth within protozoa, it is less clear whether the bacteria form or replicate within biofilms in the absence of protozoa. in this study, we analyzed surface adherence of and biofilm formation by l. pneumophila in a rich medium that supported axenic replication. biofilm formation by t ... | 2006 | 16597995 |
| diverse bacteria associated with root nodules of spontaneous legumes in tunisia and first report for nifh-like gene within the genera microbacterium and starkeya. | we characterized 34 endophytic bacterial isolates associated to root nodules collected from spontaneous legumes in the arid zone of tunisia by 16s rdna polymerase chain reaction (pcr)-restriction fragment length polymorphism, whole cell protein sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page), 16s rdna and 16s-23s rdna internal transcribed spacer sequencing. phylogenetically, these isolates belong to the branches containing the genera inquilinus, bosea, rhodopseudomonas, para ... | 2006 | 16598639 |
| survival of surface ripening cultures during storage and monitoring their development on cheese. | to study the survival of bacteria isolated from the surface of smear cheese and monitor their development during cheese ripening. | 2006 | 16599999 |
| the hydantoin transport protein from microbacterium liquefaciens. | the gene hyup from microbacterium liquefaciens aj 3912 with an added his6 tag was cloned into the expression plasmid pttq18 in an escherichia coli host strain. the transformed e. coli showed transport of radioisotope-labeled 5-substituted hydantoins with apparent k(m) values in the micromolar range. this activity exhibited a ph optimum of 6.6 and was inhibited by dinitrophenol, indicating the requirement of energy for the transport system. 5-indolyl methyl hydantoin and 5-benzyl hydantoin were t ... | 2006 | 16621827 |
| screening for microbial markers in miocene sediment exposed during open-cast brown coal mining. | viable microorganisms were found in miocene lacustrine clays of the cypris formation excavated from 200-m below the surface as spoil during open-cast brown coal mining (sokolov brown coal basin, north-western bohemia, czech republic). both saprotrophic microfungi of the genera penicillium, verticillium, cladosporium and aspergillus as well as heterotrophic bacteria were isolated from an intact sediment cores. heterotrophic bacteria were classified by the mis sherlock system as representatives of ... | 2006 | 16622790 |
| rapid analysis of two food-borne microbial communities at the species level by fourier-transform infrared microspectroscopy. | the species composition of microbial communities in natural habitats may be extremely complex and therefore a quantitative analysis of the fraction each species contributes to the consortium has proven to be difficult. during recent years, the identification of bacterial pure cultures based on their infrared spectra has been established. fourier-transform infrared microspectroscopy now proceeds a step further and allows identification of microorganisms directly plated from community dilutions. i ... | 2006 | 16623742 |
| the phylogenetic significance of peptidoglycan types: molecular analysis of the genera microbacterium and aureobacterium based upon sequence comparison of gyrb, rpob, reca and ppk and 16srrna genes. | the type strains of 27 species of the genus microbacterium, family microbacteriaceae, were analyzed with respect to the phylogeny of the housekeeping genes coding for dna gyrase subunit b (gyrb), rna-polymerase subunit b (rpob), recombinase a (reca) and polyphosphate kinase (ppk). the resulting gene trees were compared to the 16s rrna gene phylogeny of the same species. the topology of neighbour-joining and maximum parsimony phylogenetic trees based upon nucleic acid sequences and protein sequen ... | 2007 | 16684595 |
| enhanced biofilm formation and increased resistance to antimicrobial agents and bacterial invasion are caused by synergistic interactions in multispecies biofilms. | most biofilms in their natural environments are likely to consist of consortia of species that influence each other in synergistic and antagonistic manners. however, few reports specifically address interactions within multispecies biofilms. in this study, 17 epiphytic bacterial strains, isolated from the surface of the marine alga ulva australis, were screened for synergistic interactions within biofilms when present together in different combinations. four isolates, microbacterium phyllosphaer ... | 2006 | 16751497 |
| sediment microbes of deep-sea bioherms on the northwest shelf of australia. | the northwest shelf of australia is a region with known petroleum reserves. recent geological investigations have revealed carbonate knolls postulated to be of biological origin. sediment microbial populations at three sites on the northwest shelf, pee shoal, mermaid reef, and scott reef, were investigated for the presence of hydrocarbon-degrading bacteria. from two sampling trips, 246 bacterial strains were purified, and 182 of these strains displayed preferential growth on hydrocarbon-selectiv ... | 2003 | 12739077 |
| contamination of bone marrow products with an actinomycete resembling microbacterium species and reinfusion into autologous stem cell and bone marrow transplant recipients. | bacterial contamination of bone marrow or peripheral blood stem cell transplant products typically occurs with skin flora or, rarely, gram-negative organisms. we describe a clonal outbreak of contamination in transplant products caused by contamination with an aerobic actinomycete that occurred at our institution during the summer of 2001. from 1 july through 12 september 2001, 73 peripheral blood or bone marrow stem cell products were obtained from 39 patients, and 34 products were found to be ... | 2003 | 12746790 |
| pair-dependent co-aggregation behavior of non-flocculating sludge bacteria. | two strains of non-flocculating sewage sludge bacteria (xanthomonas sp. s53 and microbacterium esteraromaticum s51) showed 91% and 77% co-aggregation, respectively, with acinetobacter johnsonii s35 using a spectrophometric assay. the co-aggregates in case of xanthomonas sp. s53 and a. johnsonii s35 were above 100 microm and stable against edta (2 mm) and a commercial protease (0.2 mg ml(-1)). protease/periodate pretreatment of the partners did not affect this co-aggregation. on the other hand, c ... | 2003 | 12889835 |
| substrate induction and statistical optimization for the production of chitosanase from microbacterium sp. ou01. | the chitosanase production was markedly enhanced by substrate induction, statistical optimization of medium composition and culture conditions by microbacterium sp. ou01 in shake-flask. a significant influence of (nh(4))(2)so(4), mgso(4).7h(2)o and initial ph on chitosanase production was noted with plackett-burman design. it was then revealed with the method of steepest ascent and response surface methodology (rsm) that 19.0g/l (nh(4))(2)so(4), 1.3g/l mgso(4) and an initial ph of 2.0 were optim ... | 2007 | 16930999 |
| identification of novel eubacteria from spent mushroom compost (smc) waste by dna sequence typing: ecological considerations of disposal on agricultural land. | a small study was undertaken to examine the microbiological characteristics of spent mushroom compost (smc), which is the major waste by-product of the mushroom industry and which is regularly disposed off by application to agricultural land. the primary aim of this study was to examine smc for the presence of faecal bacterial pathogens, including campylobacter spp., salmonella spp. and listeria monocytogenes. secondly it was desirable to quantify bacterial and fungal populations within smc, and ... | 2004 | 14672727 |
| microbacterium ulmi sp. nov., a xylanolytic, phosphate-solubilizing bacterium isolated from sawdust of ulmus nigra. | a xylanolytic and phosphate-solubilizing bacterium isolated from sawdust of ulmus nigra in salamanca was characterized by a polyphasic approach. the novel strain, designated xil02(t), was gram-positive, aerobic, catalase- and oxidase-negative, rod-shaped and non-motile. phylogenetically and chemotaxonomically, it was related to members of the genus microbacterium. according to 16s rrna gene sequence analysis, it is closely related to microbacterium arborescens and microbacterium imperiale; howev ... | 2004 | 15023968 |
| biofilm formation characteristics of bacterial isolates retrieved from a reverse osmosis membrane. | high-quality water purification systems using reverse osmosis (ro) membrane separation have faced a major challenge related to biofilm formation on the membrane surface, or biofouling. to understand this issue, the biofilm formation characteristics of four bacterial isolates previously retrieved from an ro membrane treating potable water were investigated. biofilm formation of all four isolates occurred to different extents in microtiter plates and could be related to one or more cell properties ... | 2005 | 16245826 |
| characterization of the novel hch-degrading strain, microbacterium sp. itrc1. | a gram-positive microbacterium sp. strain, itrc1, that was able to degrade the persistent and toxic hexachlorocyclohexane (hch) isomers was isolated and characterized. the itrc1 strain has the capacity to degrade all four major isomers of hch present in both liquid cultures and aged contaminated soil. dna fragments corresponding to the two initial genes involved in gamma-hch degradative pathway, encoding enzymes for gamma-pentachlorocyclohexene hydrolytic dehalogenase (linb) and a 2,5-dichloro-2 ... | 2006 | 16315057 |
| purification and properties of a keratinolytic metalloprotease from microbacterium sp. | this study was developed to purify and to characterize a keratinolytic protease from the bacterium microbacterium sp. strain kr10. | 2006 | 17105556 |
| activities and survival of endophytic bacteria in white clover (trifolium repens l.). | in this study, the genera, abundance, and activities of endophytic bacteria in field-grown white clover (trifolium repens) and the fate of introduced antibiotic-tolerant bacteria in white clover tissues were investigated. pseudomonas, pantoea, and corynebacterium were the most frequently isolated endophytic bacteria genera, whereas xanthomonas, microbacterium, and cellulomonas occurred less frequently. the average bacterial populations in stolons and roots were approximately 100,000 colony-formi ... | 2006 | 17110977 |
| molecular diversity studies of bacterial communities of oil polluted microbial mats from the etang de berre (france). | the biodiversity of microbial mats inhabiting the oil-contaminated lagoon etang de berre was determined by molecular approaches. the fingerprint of denaturing gradient gel electrophoresis (dgge) and automatic ribosomal intergenic spacer analysis (arisa) of mats exposed to different pollution levels showed specific microbial communities for each site but similar diversity richness. species composition of the mats were compared by constructing 16s rrna libraries. amplified rdna restriction analysi ... | 2006 | 17117996 |
| symbiotic association in chlorella culture. | chlorella sorokiniana iam c-212 has long been maintained in slant culture as a mixed strain, representing an associated natural microbial consortium. in this study, the consortium was separated and five nonalgal constituents, a fungal strain (cssf-1), and four bacterial strains (cssb-1, cssb-2, cssb-3, and cssb-4) were isolated and identified. 16s rdna sequence analysis revealed that strains cssb-1, cssb-2, cssb-3, and cssb-4 were close to ralstonia pickettii (99.8% identity), sphingomonas sp. d ... | 2005 | 16329867 |
| characterization of extracellular polymers synthesized by tropical intertidal biofilm bacteria. | this study was performed to determine the potential of tropical intertidal biofilm bacteria as a source of novel exopolymers (eps). | 2007 | 17184342 |
| chilling and cultivar type affect the diversity of bacterial endophytes colonizing sweet pepper (capsicum anuum l.). | a climate chamber experiment was conducted to assay the effect of low temperatures (chilling) on the diversity of bacteria colonizing the endospheres of two thermophilic sweet pepper (capsicum anuum l.) cultivars, milder spiral and ziegenhorn bello. structural diversity was analyzed by 16s rrna-based terminal restriction fragment length polymorphism (t-rflp) analysis and by the generation of 16s rrna gene libraries to determine dominant community members in t-rflp profiles. cultivable community ... | 2006 | 17215894 |
| a new microbacterium species isolated from the blood of a patient with fever: microbacterium pyrexiae sp. nov. | a gram-positive bacterium, smc-a8265(t), which was isolated from the blood of a patient with fever but could not be identified by a conventional microbiologic method, was finally characterized by performing phenotypic and genotypic analyses. 16s rrna gene sequence analysis revealed that the strain smc-a8265(t) belonged to the genus microbacterium, but it did not correspond to any of the previously described microbacterium spp. biochemical tests and cellular fatty acid composition analysis also c ... | 2007 | 17240108 |
| nutritional requirements of microbacterium thermosphactum. | microbacterium thermosphactum requires cysteine, alpha-lipoate, nicotinate, pantothenate, p-aminobenzoate, biotin, and thiamin for aerobic growth in glucose-mineral salts medium. glucose cannot be replaced by casamino acids or acids of the tricarboxylic acid cycle as sole carbon and energy sources. the organism can also grow anaerobically in the minimal synthetic medium. | 1979 | 16345460 |
| heavy metal resistance and genotypic analysis of metal resistance genes in gram-positive and gram-negative bacteria present in ni-rich serpentine soil and in the rhizosphere of alyssum murale. | forty-six bacterial cultures, including one culture collection strain, thirty from the rhizosphere of alyssum murale and fifteen from ni-rich soil, were tested for their ability to tolerate arsenate, cadmium, chromium, zinc, mercury, lead, cobalt, copper, and nickel in their growth medium. the resistance patterns, expressed as minimum inhibitory concentrations, for all cultures to the nine different metal ions were surveyed by using the agar dilution method. a large number of the cultures were r ... | 2007 | 17276484 |
| microbacterium paludicola sp. nov., a novel xylanolytic bacterium isolated from swamp forest. | a xylanolytic bacterium, us15t, was isolated from swamp forest soil in ulsan, korea. the cells of the novel strain were gram-positive, non-motile, short-rod-shaped and showed chemotaxonomic properties that were consistent with its classification in the genus microbacterium. chemotaxonomic results showed mk-12 and mk-11 as major menaquinones, predominating iso- and anteiso-branched cellular fatty acids, glucose, galactose and mannose as cell-wall sugars, peptidoglycan-type b2beta with glycolyl re ... | 2006 | 16514023 |
| caenorhabditis elegans mutants resistant to attachment of yersinia biofilms. | the detailed composition and structure of the caenorhabditis elegans surface are unknown. previous genetic studies used antibody or lectin binding to identify srf genes that play roles in surface determination. infection by microbacterium nematophilum identified bus (bacterially unswollen) genes that also affect surface characteristics. we report that biofilms produced by yersinia pestis and y. pseudotuberculosis, which bind the c. elegans surface predominantly on the head, can be used to identi ... | 2007 | 17339204 |
| [evaluation of api coryne system, version 2.0, for diphteroid gram-positive rods identification with clinical relevance]. | the ability of the api coryne system, version 2.0, to identify 178 strains of gram-positive rods was evaluated. seventy eight isolates belonged to genus corynebacterium and one hundred to related genera, all strains were isolated from clinical samples at the laboratory of bacteriology, hospital de clínicas josé de san martin (uba) between 1995 and 2004. the isolates were identified according to von graevenitz and funke's scheme. one hundred and sixty two out of 178 strains (91%) were correctly i ... | 2006 | 17370571 |
| bacterial diversity in a nonsaline alkaline environment: heterotrophic aerobic populations. | heterotrophic populations were isolated and characterized from an alkaline groundwater environment generated by active serpentinization, which results in a ca(oh)2-enriched, extremely diluted groundwater with ph 11.4. one hundred eighty-five strains were isolated in different media at different ph values during two sampling periods. to assess the degree of diversity present in the environment and to select representative strains for further characterization of the populations, we screened the is ... | 2004 | 15574939 |
| metabolic activity and phylogenetic diversity of reed (phragmites australis) periphyton bacterial communities in a hungarian shallow soda lake. | in the present study, the species composition and potential metabolic activities of bacterial communities of reed phragmites australis (cav.) (trin. ex steudel) periphyton from lake velencei were studied by cultivation-based and metabolic fingerprinting methods. serially diluted spring biofilm samples were used to test the community-level physiological profiling (clpp) using biolog microplates, and for plating onto different media. on the basis of their morphological, biochemical, and physiologi ... | 2007 | 17406774 |
| [reaction of microorganisms to the digestive fluid of the earthworms]. | the reaction of soil bacteria and fungi to the digestive fluid of the earthworm aporrectodea caliginosa was studied. the fluid was obtained by centrifugation of the native enzymes of the digestive tract. the inhibition of growth of certain bacteria, spores, and fungal hyphae under the effect of extracts from the anterior and middle sections of the digestive tract of a. caliginosa was discovered for the first time. in bacteria, microcolony formation was inhibited as early as 20-30 s after the app ... | 2007 | 17410875 |
| culture-independent analysis of bacterial diversity in a child-care facility. | child-care facilities appear to provide daily opportunities for exposure and transmission of bacteria and viruses. however, almost nothing is known about the diversity of microbial contamination in daycare facilities or its public health implications. recent culture-independent molecular studies of bacterial diversity in indoor environments have revealed an astonishing diversity of microorganisms, including opportunistic pathogens and many uncultured bacteria. in this study, we used culture and ... | 2007 | 17411442 |
| isolation and identification of potential phosphate solubilizing bacteria from the rhizoplane of oryza sativa l. cv. br29 of bangladesh. | a total of 30 bacteria were isolated from the rhizoplane of rice cv. br29 cultivated in mymensingh, bangladesh and from the seedlings obtained from surface-sterilized seeds of br29. upon screening, 6 isolates showed varying levels of phosphate solubilizing activity in both agar plate and broth assays using national botanical research institute's phosphate medium. the bacterial isolates were identified based on their phenotypic and 16s rrna genes sequencing data as acinetobacter sp. br-12, klebsi ... | 2007 | 17425114 |
| effects of ph amendment on metal working fluid wastewater biological treatment using a defined bacterial consortium. | the aim of this study was to determine whether ph amendment of a highly alkaline metal working fluid (mwf) wastewater would improve biological treatment in a bioreactor system following introduction of a bacterial inoculum (comprised of the following strains: agrobacterium radiobacter, comamonas testosteroni, methylobacterium mesophilicum, microbacterium esteraromaticum, and microbacterium saperdae). the ph values tested were 6, 7, 8, and 9. three replicate batch mode bioreactors inoculated with ... | 2005 | 15625673 |
| microbacterium halotolerans sp. nov., isolated from a saline soil in the west of china. | a gram-positive short rod isolated from a saline soil in china was characterized using a polyphasic approach. this actinobacterium grew over a wide salinity range [0-15 % nacl, 0-20 % kcl and 0-30 % (mgcl2).6h2o (w/v); optimum concentrations for growth were 5 % nacl, 5-10 % kcl, 10 % (mgcl2).6h2o]. the optimum growth temperature and ph were 28-30 degrees c and 7.0-8.0, respectively. chemotaxonomic features (peptidoglycan-type b2beta with glycolyl residues; major menaquinones mk-10 and mk-11; pre ... | 2005 | 15653854 |
| isolation and characterization of a xanthan-degrading microbacterium sp. strain xt11 from garden soil. | isolation and characterization of the xanthan-degrading microbacterium sp. xt11. | 2007 | 17448171 |
| expression and purification of a recombinant enantioselective amidase. | microbacterium sp. aj115 metabolises a wide range of nitriles using the two-step nitrile hydratase/amidase pathway. in this study, the amidase gene of microbacterium sp. aj115 has been inserted into the pcal-n-ek expression vector and expressed in escherichia coli bl21(de3)plyss. the expressed protein is active in e. coli and expression of the amidase gene allows e. coli to grow on acetamide as sole carbon and/or nitrogen source. expression of active amidase in e. coli was temperature dependent ... | 2005 | 15721788 |
| antimicrobial properties of two purified skin peptides from the mink frog (rana septentrionalis) against bacteria isolated from the natural habitat. | numerous peptides exhibiting antimicrobial properties have been isolated from the skins of many amphibian species. these peptides offer an innate chemical defense system against various microbial agents that exist in the amphibian's environment. amphibian skin peptides are typically tested for antimicrobial activity against microbial strains that are pathogenic to humans, but not on potential pathogenic or opportunistic bacteria that exist in the organism's habitat. two peptides, a brevinin-2-re ... | 2007 | 17499556 |
| nitrile bioconversion by microbacterium imperiale cbs 498-74 resting cells in batch and ultrafiltration membrane bioreactors. | the biohydration of acrylonitrile, propionitrile and benzonitrile catalysed by the nhase activity contained in resting cells of microbacterium imperiale cbs 498-74 was operated at 5, 10 and 20 degrees c in laboratory-scale batch and membrane bioreactors. the bioreactions were conducted in buffered medium (50 mm na(2)hpo(4)/nah(2)po(4), ph 7.0) in the presence of distilled water or tap-water, to simulate a possible end-pipe biotreatment process. the integral bioreactor performances were studied w ... | 2006 | 15739103 |
| microbacterium oleivorans sp. nov. and microbacterium hydrocarbonoxydans sp. nov., novel crude-oil-degrading gram-positive bacteria. | a taxonomic study of two crude-oil-degrading, gram-positive bacterial strains, designated bas69(t) and bnp48(t), revealed that they represent two novel microbacterium species. 16s rrna gene sequence similarity to their closest phylogenetic neighbours was 98.5 % for bas69(t) (microbacterium paraoxydans dsm 15019(t) and microbacterium saperdae dsm 20169(t)) and 99 % for bnp48(t) (microbacterium luteolum dsm 20143(t)). levels of dna-dna relatedness to the closest phylogenetic neighbours of both str ... | 2005 | 15774639 |
| microbacterium natoriense sp. nov., a novel d-aminoacylase-producing bacterium isolated from soil in natori, japan. | a rod-shaped, gram-positive bacterium, strain tnjl143-2(t), having n-acyl-d-amino acid amidohydrolase (d-aminoacylase) activity, was isolated from a soil sample from natori, japan. it was a non-spore-forming, strictly aerobic bacterium without motility, showing a temperature optimum for growth of 30 degrees c and a ph optimum for growth of 5-7. the 16s rrna gene sequence of the strain showed the highest similarities to members of the genus microbacterium, in particular, microbacterium aerolatum, ... | 2005 | 15774640 |
| reduction of cr(vi) by immobilized cells of desulfovibrio vulgaris ncimb 8303 and microbacterium sp. ncimb 13776. | hexavalent chromium, a carcinogen and mutagen, can be reduced to cr(iii) by desulfovibrio vulgaris ncimb 8303 and microbacterium sp. ncimb 13776. this study examined cr(vi) reduction by immobilized cells of the two strains in a common solution matrix using various entrapment matrices. chitosan and pva-borate beads did not retain integrity and supported low or no reduction of cr(vi) by the cells. a commercial preparation (lentikats) was stable but also did not support cr(vi) reduction. k-carragee ... | 2005 | 15818565 |
| [bacterial conversion of sulfur-and phosphorous-compounds and microbial diversity in sediments from a near-shore marine-cultural region]. | the h2s-producing bacteria and the phosphorous-cycling bacteria in sediments from near-shore marine-cultural region were investigated. results indicate that the bacterial h2s production in aerobic condition is the dominating process to produce h2s in the sediment of cultural pond. the total counts of h2s-producing bacteria utilizing cysteine and na2s2o3 were 1.6 x 10(6) and 4.35 x 10(3) cells g(-1) respectively. the counts of sulfate-reducing bacteria in the sediments were very little, only 2.5 ... | 2005 | 16004320 |
| microbial consortium bioaugmentation of a polycyclic aromatic hydrocarbons contaminated soil. | in this study we evaluated the capacity of a defined microbial consortium (five bacteria: mycobacterium fortuitum, bacillus cereus, microbacterium sp., gordonia polyisoprenivorans, microbacteriaceae bacterium, naphthalene-utilizing bacterium; and a fungus identified as fusarium oxysporum) isolated from a pahs contaminated landfarm site to degrade and mineralize different concentrations (0, 250, 500 and 1000 mg kg(-1)) of anthracene, phenanthrene and pyrene in soil. pahs degradation and mineraliz ... | 2008 | 17572084 |
| purification and characterization of a maltooligosaccharide-forming amylase that improves product selectivity in water-miscible organic solvents, from dimethylsulfoxide-tolerant brachybacterium sp. strain lb25. | a bacterium that secretes maltooligosaccharide-forming amylase in a medium containing 12.5% (vol/vol) dimethylsulfoxide (dmso) was isolated and identified as brachybacterium sp. strain lb25. the amylase of the strain was purified from the culture supernatant, and its molecular mass was 60 kda. the enzyme was stable at ph 7.0-8.5 and active at ph 6.0-7.5. the optimum temperature at ph 7.0 was 35 degrees c in the presence of 5 mm cacl(2). the enzyme hydrolyzed starch to produce maltotriose primari ... | 2007 | 17619813 |
| [isolation of phenol-degrading bacteria from natural soil and their phylogenetic analysis]. | five bacterial strains capable of utilizing phenol as sole carbon source for growth were isolated from non-contaminated natural soil sample after enrichment in the presence of phenol. they were preliminarily identified according to their phylogenetic analysis and physiological and biochemical characteristics. strain phd-2, phd-4 and phd-5 belonged to the genera of ralstonia, acinetobacter and microbacterium respectively; strain phd-1 and phd-3 were from the genus of pseudomonas. homology compari ... | 2007 | 17633645 |
| isolation of bacterial endophytes from germinated maize kernels. | the germination of surface-sterilized maize kernels under aseptic conditions proved to be a suitable method for isolation of kernel-associated bacterial endophytes. bacterial strains identified by partial 16s rrna gene sequencing as pantoea sp., microbacterium sp., frigoribacterium sp., bacillus sp., paenibacillus sp., and sphingomonas sp. were isolated from kernels of 4 different maize cultivars. genus pantoea was associated with a specific maize cultivar. the kernels of this cultivar were ofte ... | 2007 | 17668041 |
| evaluation of microbial strains for linoleic acid hydroxylation and reclassification of strain ala2. | in previous studies, a new microbial strain ala2 was isolated which produced many new products from linoleic acid [gardner h.w., hou c.t., weisleder d. and brown w. 2000. lipids 35: 1055-1060; hou c.t. 1998. 12,13,17-trihydroxy-9(z)-octodecenoic acid and derivatives and microbial isolate for production of the acid. us patent no. 5, 852, 196]. strain ala2 was preliminary identified as clavibacter sp. based on its physiological and fatty acid profiles. to determine if strain ala2 is the optimal st ... | 2005 | 16096693 |
| orchid-associated bacteria produce indole-3-acetic acid, promote seed germination, and increase their microbial yield in response to exogenous auxin. | germination of orchid seeds is a complex process. in this paper we focus on interactions between the host-plant and its bacterial partners via indole-3-acetic acid (iaa). originally isolated from the roots of the epiphytic orchid dendrobium moschatum, the strains of rhizobium, microbacterium, sphingomonas, and mycobacterium genera were among the most active iaa producers. addition of exogenous tryptophan significantly enhanced auxin formation both in mineral and complex media. the presence of ia ... | 2007 | 17687544 |
| production of volatile aroma compounds by bacterial strains isolated from different surface-ripened french cheeses. | twelve bacterial strains belonging to eight taxonomic groups: brevibacterium linens, microbacterium foliorum, arthrobacter arilaitensis, staphylococcus cohnii, staphylococcus equorum, brachybacterium sp., proteus vulgaris and psychrobacter sp., isolated from different surface-ripened french cheeses, were investigated for their abilities to generate volatile aroma compounds. out of 104 volatile compounds, 54 volatile compounds (identified using dynamic headspace technique coupled with gas chromat ... | 2007 | 17701035 |
| alkaliphilic and halophilic hydrocarbon-utilizing bacteria from kuwaiti coasts of the arabian gulf. | green animate materials from the intertidal zone of the arabian gulf coast accommodated more alkaliphilic and halophilic bacteria than inanimate materials. the alkaliphilic oil-utilizing bacteria, as identified by their 16s ribonucleic acid sequences, belonged to the following genera arranged in decreasing frequences: marinobacter, micrococcus, dietzia, bacillus, oceanobacillus, and citricoccus. the halophilic oil-utilizing bacteria belonged to the genera: marinobacter, georgenia, microbacterium ... | 2007 | 17710391 |
| molecular cloning and expression of the hyu genes from microbacterium liquefaciens aj 3912, responsible for the conversion of 5-substituted hydantoins to alpha-amino acids, in escherichia coli. | a dna fragment from microbacterium liquefaciens aj 3912, containing the genes responsible for the conversion of 5-substituted-hydantoins to alpha-amino acids, was cloned in escherichia coli and sequenced. seven open reading frames (hyup, hyua, hyuh, hyuc, orf1, orf2, and orf3) were identified on the 7.5 kb fragment. the deduced amino acid sequence encoded by the hyua gene included the n-terminal amino acid sequence of the hydantoin racemase from m. liquefaciens aj 3912. the hyua, hyuh, and hyuc ... | 2005 | 16116274 |
| synthesis and characterisation of a ligand that forms a stable tetrahedral intermediate in the active site of the aureobacterium species (-) gamma-lactamase. | the crystal structure of a (-) gamma-lactamase from an aureobacterium species showed a molecule bound covalently to the active site serine residue. this enzyme complex represented the first structure of a stably bound tetrahedral intermediate for an alpha/beta hydrolase fold enzyme. the structural elucidation of tetrahedral intermediates is important for the understanding of enzymatic mechanism, substrate recognition and enzyme inhibition. in this paper, we report the synthesis and subsequent ch ... | 2005 | 16132085 |
| vitamin requirements of microbacterium lacticum orla-jensen. | | 1947 | 17797253 |
| diversity of microorganisms in decaying maize stalks revealed by a molecular method. | microbial diversity in decaying maize stalk was characterized by constructing and analyzing rrna gene clone library. total 47 otus were obtained from 82 bacterial clones, including proteobacteria (64.6%), actinobacteria (30.5%), bacteroidetes (2.4%) and firmicutes (2.4%). most proteobacterial clones were members of rhizobium, pseudomonas and stenotrophomonas. eighty-four percent of actinobacteria was related to microbacterium. only 14 otus were identified from 124 fungal clones, including ascomy ... | 2007 | 17846593 |
| [effect of pentachlorophenol (pcp) on bacterial community of an aerobic granular sludge estimated by t-rflp]. | effect of pentachlorophenol (pcp) on cod and ammonium removal rates was investigated, and also changes of bacterial community within aerobic granular sludge were monitored by terminal restriction fragment length polymorphism (t-rflp) technique. compared with the removal rate of cod, the rate of ammonium removal is more sensitive to pcp. as the ammonium and cod removal rates decreased consistently, the bacterial genera within the aerobic granular sludge also decrease with the increase of pcp conc ... | 2006 | 16768009 |
| the selection of mixed microbial inocula in environmental biotechnology: example using petroleum contaminated tropical soils. | the impact of inorganic n and p additions on a tropical soil contaminated with petroleum hydrocarbons was investigated using molecular and culture techniques. microcosms were incubated for 42 days and sampled at 0, 1, 7, 28 and 42 days. changes in bacterial community structure were determined using denaturing gradient gel electrophoresis (dgge) of the rrna following reverse transcription pcr using primers specific to the v3 region of the 16s rrna gene. to identify which components of the microbi ... | 2006 | 16226327 |
| adsorption of microorganisms onto an artificial siderophore-modified au substrate. | the hydroxamate-type artificial siderophore, tris[2-{3-(n-acetyl-n-hydroxamino)propylamido}propyl]aminomethane (tappa) and its fe(iii) complex, fe(iii)-tappa were prepared and characterized by several spectroscopic methods. fe(iii)-tappa exhibits biological activity for the hydroxamate-type siderophore auxotrophic microorganism, microbacterium flavescens, suggesting that fe(iii)-tappa can permeate the cell membrane of the microorganism. the adsorption of the fe(iii)-siderophore complex onto a de ... | 2007 | 17890077 |
| culturable psychrotrophic bacterial communities in raw milk and their proteolytic and lipolytic traits. | during cold storage after milk collection, psychrotrophic bacterial populations dominate the microflora, and their extracellular enzymes, mainly proteases and lipases, contribute to the spoilage of dairy products. the diversity, dynamics, and enzymatic traits of culturable psychrotrophs in raw milk from four farms were investigated over a 10-month period. about 20% of the isolates were found to be novel species, indicating that there is still much to be learned about culturable psychrotrophs in ... | 2007 | 17890340 |
| diversity of cultivated endophytic bacteria from sugarcane: genetic and biochemical characterization of burkholderia cepacia complex isolates. | bacteria were isolated from the rhizosphere and from inside the roots and stems of sugarcane plants grown in the field in brazil. endophytic bacteria were found in both the roots and the stems of sugarcane plants, with a significantly higher density in the roots. many of the cultivated endophytic bacteria were shown to produce the plant growth hormone indoleacetic acid, and this trait was more frequently found among bacteria from the stem. 16s rrna gene sequence analysis revealed that the select ... | 2007 | 17905875 |
| glyceroglycolipids preventing tert-butylhydroperoxide-induced cell death from microbacterium sp. and corynebacterium aquaticum strains. | galactosyl diacylglycerols m874b and s365b obtained from the recently isolated bacteria identified as microbacterium sp. m874 and corynebacterium aquaticum s365 were found to prevent oxidative cell death induced by tert-butylhydroperoxide. their structures were determined to be 1,2-di-o-(12-methyltetradecanoyl)-3-o-beta-d-galactopyranosyl-sn-glycerol and 1-o-(14-methylhexadecanoyl)-2-o-(12-methyltetradecanoyl)-3-o-beta-d-galactopyranosyl-sn-glycerol, respectively. | 2000 | 16232721 |
| genomic clusters, putative pathogen recognition molecules, and antimicrobial genes are induced by infection of c. elegans with m. nematophilum. | the interaction between the nematode caenorhabditis elegans and a gram-positive bacterial pathogen, microbacterium nematophilum, provides a model for an innate immune response in nematodes. this pathogen adheres to the rectal and post-anal cuticle of the worm, causing slowed growth, constipation, and a defensive swelling response of rectal hypodermal cells. to explore the genomic responses that the worm activates after pathogenic attack we used microarray analysis of transcriptional changes indu ... | 2006 | 16809667 |
| purification and characterization of two types of chitosanase from a microbacterium sp. | two extracellular chitosanases (chix and chin) were extracted from microbacterium sp. ou01 with mr values of 81 kda (chix) and 30 kda (chin). chin was optimally active at ph 6.2 and 50 degrees c and chix at ph 6.6 and 60 degrees c (assayed over 15 min). both the activities increased with the degree of deacetylation (dda) of chitosan. chin hydrolyzed oligomers of glucosamine (glcn) larger than chitopentaose, and chitosan with 62-100% dda; but chix acted on chitosan and released glcn. hydrolysis o ... | 2006 | 16820974 |
| interactions with microbial pathogens. | a wide variety of bacterial pathogens, as well as several fungi, kill c. elegans or produce non-lethal disease symptoms. this allows the nematode to be used as a simple, tractable model host for infectious disease. human pathogens that affect c. elegans include gram-negative bacteria of genera burkholderia, pseudomonas, salmonella, serratia and yersinia; gram-positive bacteria enterococcus, staphylococcus and streptococcus; and the fungus cryptococcus neoformans. microbes that are not pathogenic ... | 2005 | 18050390 |
| degradation of phenanthrene on plant leaves by phyllosphere bacteria. | the activity of phyllosphere bacteria in the degradation of phenanthrene was investigated as a mechanism for the removal of atmospheric phenanthrene after its deposition on plant leaves. initially, leaf samples of six plant species were collected from two roadsides in bangkok to determine the presence of phenanthrene-degrading bacteria. the numbers of phenanthrene-degrading phyllosphere bacteria were varied and ranged from 3.5 x 10(4) to 1.95 x 10(7) cfu/g, in which the highest number was found ... | 2007 | 18057816 |
| degradation of environmental endocrine disruptor di-2-ethylhexyl phthalate by a newly discovered bacterium, microbacterium sp. strain cq0110y. | in this study di-2-ethylhexyl phthalate (dehp)-degradation strain cq0110y was isolated from activated sludge. according to the biophysical/biochemical characteristics and analysis of 16s rdna, the strain was identified as microbacterium sp. the results of this study showed the optimal ph value and optimal temperature which influenced the degradation rate in wastewater: ph 6.5-7.5, 25-35 degrees c. kinetics of degradation reaction had been performed at different initial concentrations and differe ... | 2007 | 17089119 |
| [microbial diversity in scorpion intestine (buthus martensii karsch)]. | scorpion is an important officinal animal, and has a high nutritional value. in this study, the culture-independent and culture-dependent methods were used to investigate the microbial diversity in the scorpion's intestine. results based on culture-independent method showed the bacteria to be related to alpha, beta, gamma-proteobacteria. bacteria isolated by the culture-dependent method were high g + c, gram-positive bacteria. the genera enterobacter, serratia and ochrobactrum were detected by b ... | 2007 | 18062268 |
| effect of shadowing on survival of bacteria under conditions simulating the martian atmosphere and uv radiation. | spacecraft-associated spores and four non-spore-forming bacterial isolates were prepared in atacama desert soil suspensions and tested both in solution and in a desiccated state to elucidate the shadowing effect of soil particulates on bacterial survival under simulated martian atmospheric and uv irradiation conditions. all non-spore-forming cells that were prepared in nutrient-depleted, 0.2-microm-filtered desert soil (dse) microcosms and desiccated for 75 days on aluminum died, whereas cells p ... | 2008 | 18083857 |
| the growth of microbacterium lacticum in a chemically defined medium. | | 1949 | 18108881 |
| infections caused by coryneform bacteria in pediatric oncology patients. | invasive infections caused by coryneform bacteria are uncommon but have been reported with increasing frequency in recent decades, especially in immunocompromised persons. because pediatric experience is limited, we examined the epidemiology and clinical characteristics of these infections in children undergoing cancer therapy. | 2008 | 18174873 |
| mos1 mutagenesis reveals a diversity of mechanisms affecting response of caenorhabditis elegans to the bacterial pathogen microbacterium nematophilum. | a specific host-pathogen interaction exists between caenorhabditis elegans and the gram-positive bacterium microbacterium nematophilum. this bacterium is able to colonize the rectum of susceptible worms and induces a defensive tail-swelling response in the host. previous mutant screens have identified multiple loci that affect this interaction. some of these loci correspond to known genes, but many bus genes [those with a bacterially unswollen (bus) mutant phenotype] have yet to be cloned. we em ... | 2007 | 17151260 |
| identification of diazotrophs in the culturable bacterial community associated with roots of lasiurus sindicus, a perennial grass of thar desert, india. | lasiurus sindicus is a highly nutritive, drought-tolerant, perennial grass that is endemic to the thar desert of rajasthan, india. analysis of 16s rrna coding genes of the bacterial isolates enriched in nitrogen-free semisolid medium, from the surface-sterilized roots of l. sindicus, showed predominance of gram-negative over gram-positive bacteria. according to comparative sequence analysis of 16s rdna sequence data, gram-positive bacteria with low gc content (staphylococcus warneri and bacillus ... | 2007 | 17264993 |
| 17beta-estradiol-degrading bacteria isolated from activated sludge. | fourteen phylogenetically diverse 17beta-estradiol-degrading bacteria (strains kc1-14) were isolated from activated sludge of a wastewater treatment plant. these isolates widely distributed among eight different genera--aminobacter (strains kc6 and kc7), brevundimonas (strain kc12), escherichia (strain kc13), flavobacterium (strain kc1), microbacterium (strain kc5), nocardioides (strain kc3), rhodococcus (strain kc4), and sphingomonas (strains kc8-kc11 and kc14)--of three phyla: proteobacteria, ... | 2007 | 17310711 |
| assessment of microbial diversity in effluent treatment plants by culture dependent and culture independent approaches. | microbial community structure of two distinct effluent treatment plants (etps) of pesticide and pharmaceutical industries was assessed and defined by (i) culture dependent and culture independent approaches on the basis of 16s rrna gene sequencing, (ii) diversity index analysis - operational taxonomic units (otus). a total of 38 and 44 bacterial otus having 85-99% similarity with the closest match in the database were detected among pharmaceutical and pesticide sludge samples, respectively. fift ... | 2008 | 18280146 |
| microbacterium isolates from the vicinity of a radioactive waste depository and their interactions with uranium. | three oligotrophic bacterial strains were cultured from the ground water of the deep-well monitoring site s15 of the siberian radioactive waste depository tomsk-7, russia. they were affiliated with actinobacteria from the genus microbacterium. the almost fully sequenced 16s rrna genes of two of the isolates, s15-m2 and s15-m5, were identical to those of cultured representatives of the species microbacterium oxydans. the third isolate, s15-m4, shared 99.8% of 16s rrna gene identity with them. the ... | 2007 | 17381522 |
| microbacterium hatanonis sp. nov., isolated as a contaminant of hairspray. | an aerobic, rod-shaped, gram-positive, oxidase-negative, catalase-positive bacterial isolate, strain fcc-01(t), originating as a contaminant of hairspray was characterized using phenotypic and molecular taxonomic methods. a 16s rrna gene sequence analysis revealed that the isolate belonged to the genus microbacterium and represented an evolutionary lineage that was distinct from recognized microbacterium species. cell-wall hydrolysate from the isolate contained ornithine and the cell-wall sugars ... | 2008 | 18319473 |
| characterization of a cryptic plasmid from a greenland ice core arthrobacter isolate and construction of a shuttle vector that replicates in psychrophilic high g+c gram-positive recipients. | over 60 greenland glacial isolates were screened for plasmids and antibiotic resistance/sensitivity as the first step in establishing a genetic system. sequence analysis of a small, cryptic, 1,950 bp plasmid, p54, from isolate gic54, related to arthrobacter agilis, showed a region similar to that found in theta replicating rhodococcus plasmids. a 6,002 bp shuttle vector, psvj21, was constructed by ligating p54 and puc18 and inserting a chloramphenicol acetyl transferase (cat) cassette conferring ... | 2008 | 18335166 |
| phylogenetic and enzymatic diversity of deep subseafloor aerobic microorganisms in organics- and methane-rich sediments off shimokita peninsula. | "a meta-enzyme approach" is proposed as an ecological enzymatic method to explore the potential functions of microbial communities in extreme environments such as the deep marine subsurface. we evaluated a variety of extra-cellular enzyme activities of sediment slurries and isolates from a deep subseafloor sediment core. using the new deep-sea drilling vessel "chikyu", we obtained 365 m of core sediments that contained approximately 2% organic matter and considerable amounts of methane from offs ... | 2008 | 18368287 |
| genetic diversity of endophytic bacteria which could be find in the apoplastic sap of the medullary parenchym of the stem of healthy sugarcane plants. | the genetic diversity of 29 endophytic bacterial strains isolated from apoplastic sap of the medullary parenchym of the stem of healthy sugarcane plants grown in cuba was analysed by two primers-ramdom amplified polymorphic dna fingerprinting (tp-rapd) and 16s rrna gene sequencing. the strains were distributed into 17 groups on the basis of their tp-rapd patterns, and a representative strain from each group was subjected to 16s rrna gene sequencing. analysis of these sequences showed that the is ... | 2008 | 18383223 |
| chemostat selection of a bacterial community able to degrade s-triazinic compounds: continuous simazine biodegradation in a multi-stage packed bed biofilm reactor. | using a successive transfer method on mineral salt medium containing simazine, a microbial community enriched with microorganisms able to grow on simazine was obtained. afterwards, using a continuous enrichment culture procedure, a bacterial community able to degrade simazine from an herbicide formulation was isolated from a chemostat. the continuous selector, fed with a mineral salt medium containing simazine and adjuvants present in the commercial herbicide formulation, was maintained in opera ... | 2008 | 18392868 |
| microbacterium terricolae sp. nov., isolated from soil in japan. | the taxonomic positions of two novel strains isolated from a soil sample collected in japan using glucose-peptone-meat extract (gpm) agar plates supplemented with superoxide dismutase or superoxide dismutase plus catalase were investigated based on the results of chemotaxonomic, phenotypic and genotypic characteristics. strains were gram-positive, catalase-positive, non-motile bacteria with l-ornithine as a diagnostic diamino acid of the peptidoglycan. the acyl type of the peptidoglycan was n-gl ... | 2007 | 17429156 |
| stability of the biodiversity of the surface consortia of gubbeen, a red-smear cheese. | a total of 1,052 bacteria and 828 yeasts were isolated from the surface flora of 6 batches of gubbeen cheese made in 1996-1997 and 2002-2003. stability of the microflora was evaluated over time and also during ripening at 4, 10, and 16 d (batches 4, 5, and 6) or at 4, 16, 23, and 37 d (batches 1, 2, and 3). bacteria were identified using pulsed-field gel electrophoresis, repetitive extragenic palindromic-pcr, and 16s rrna gene sequencing, and yeasts were identified by fourier transform infrared ... | 2007 | 17430918 |
| screening and distributing features of bacteria with hydantoinase and carbamoylase. | hydantoinase and carbamoylase are key biocatalysts for the production of optically pure amino acids from dl-5-substituted hydantoins (ssh). out of 364 isolated strains with hydantoinase and carbamoylase at 45 degrees c, 24 strains showed efficient hydantoinase and carbamoylase activities. among them, 17 produced l-amino acids, and 7 produced d-amino acids from both aromatic dl-5-benzylhydantoin and aliphatic dl-5-isopropylhydantoin. most of the strains that were able to form l-amino acid belonge ... | 2009 | 17498938 |
| microbacterium aquimaris sp. nov., isolated from seawater. | strains js54-2(t) and js63-1 were isolated from seawater at jeju, korea. cells of these strains were gram-positive, non-motile, non-spore-forming, short rods and formed yellow-pigmented colonies on tryptic soy agar. the strains had chemotaxonomic markers that were consistent with their classification in the genus microbacterium, i.e. mk-11, mk-12 and mk-10 as the major menaquinones, fatty acids that were predominantly iso- and anteiso-branched, galactose and glucose as the cell-wall sugars, pept ... | 2008 | 18599704 |
| microbacterium luticocti sp. nov., isolated from sewage sludge compost. | strain sc-087b(t), isolated from sewage sludge compost during a study of bacterial diversity in composts, was characterized. the isolate was a gram-positive, short rod that was motile, catalase- and oxidase-negative and able to grow at 27-45 degrees c, ph 5.5-9.7 and in up to 10 % nacl. the peptidoglycan was of the b2beta type, containing the characteristic amino acids ornithine, homoserine and hydroxyglutamic acid. the muramic acid residues of the peptidoglycan were partially glycolylated. the ... | 2008 | 18599719 |
| a novel dps-type protein from insect gut bacteria catalyses hydrolysis and synthesis of n-acyl amino acids. | a novel type of a microbial n-acyl amino acid hydrolase (aah) from insect gut bacteria was purified, cloned and functionally characterized. the enzyme was obtained from microbacterium arborescens se14 isolated from the foregut of larvae of the generalist herbivore spodoptera exigua. the substrates of aah are n-acyl-glutamines previously reported to elicit plant defence reactions after introduction into the leaf during feeding. the isolated aah catalyses the hydrolysis of the amide bond (k(m) = 3 ... | 2007 | 17504494 |
| treatment of bromoamine acid wastewater using combined process of micro-electrolysis and biological aerobic filter. | the wastewater originated from the production of bromoamine acid was treated in a sequential system of micro-electrolysis (me) and biological aerobic filter (baf). decolorization and cod(cr) removal rate of the proposed system was investigated with full consideration of the influence of two major controlling factors such as organic loading rate (olr) and hydraulic retention time (hrt). the removal rate of cod(cr) was 81.2% and that of chrominance could be up to 96.6% at an olr of 0.56 kg m(-3)d( ... | 2009 | 18621476 |
| identities of arthrobacter spp. and arthrobacter-like bacteria encountered in human clinical specimens. | after the initial description of arthrobacter spp. isolated from clinical specimens in the mid-1990s, very few further reports on arthrobacter spp. have appeared in the clinical microbiology literature. the aim of the present study was to elucidate the distribution of arthrobacter spp. and arthrobacter-like bacteria encountered in clinical specimens by studying 50 consecutively isolated or received strains of large-colony-forming, whiteish-grayish, non-cheese-like-smelling, nonfermentative gram- ... | 2008 | 18650355 |
| culture-independent nested pcr method reveals high diversity of actinobacteria associated with the marine sponges hymeniacidon perleve and sponge sp. | a culture-independent nested polymerase chain reaction (pcr) technique was used to investigate the diversity of actinobacteria communities associated with the sponges hymeniacidon perleve and sponge sp. the phylogenetic affiliation of sponge-derived actinobacteria was then assessed by 16s rrna sequencing of cloned dna fragments. a total of 196 positive clones were screened by restriction fragment length polymorphism (rflp) analysis; 48 unique operational taxonomic units (otus) were selected for ... | 2008 | 18670903 |
| chromium (vi) reduction in activated sludge bacteria exposed to high chromium loading. | a mixed-culture of bacteria collected from a wastewater treatment plant in brits, north-west province (south africa) biocatalytically reduced cr(vi) at much higher concentrations than previously observed in cultures isolated in north america. complete cr(vi) reduction in aerobic cultures was achieved at a high concentration of 200 mg/l after incubation for only 65 hours. under anaerobic conditions up to 150 mg, cr(vi)/l was completely removed after incubating for 130 to 155 hours, still higher t ... | 2008 | 18701792 |
| coevolution between attine ants and actinomycete bacteria: a reevaluation. | we reassess the coevolution between actinomycete bacteria and fungus-gardening (attine) ants. actinomycete bacteria are of special interest because they are metabolic mutualists of diverse organisms (e.g., in nitrogen-fixation or antibiotic production) and because pseudonocardia actinomycetes are thought to serve disease-suppressing functions in attine gardens. phylogenetic information from culture-dependent and culture-independent microbial surveys reveals (1) close affinities between free-livi ... | 2008 | 18752608 |
| chromium (vi) reduction in activated sludge bacteria exposed to high chromium loading: brits culture (south africa). | a mixed-culture of bacteria collected from a wastewater treatment plant in brits, north-west province (south africa) biocatalytically reduced cr(vi) at much higher concentrations than previously observed in cultures isolated in north america. cr(vi) reduction rate up to 8 times higher than the rate in previous cultures was achieved by the brits culture under aerobic conditions. near complete cr(vi) reduction was observed in batches under initial concentrations up to 200mg cr(vi)/l after incubati ... | 2008 | 18760438 |
| papaya shoot tip associated endophytic bacteria isolated from in vitro cultures and host-endophyte interaction in vitro and in vivo. | fourteen distinct bacterial clones were isolated from surface-sterilized shoot tips (approximately 1 cm) of papaya (carica papaya l. 'surya') planted on murashige and skoog (ms)-based papaya culture medium (23/50 nos.) during the 2-4 week period following in vitro culturing. these isolates were ascribed to six gram-negative genera, namely pantoea (p. ananatis), enterobacter (e. cloacae), brevundimonas (b. aurantiaca), sphingomonas, methylobacterium (m. rhodesianum), and agrobacterium (a. tumefac ... | 2007 | 17538647 |
| gene sequence phylogenies of the family microbacteriaceae. | the type strains of 32 species of 13 genera of the family microbacteriaceae were analysed with respect to gene-coding phylogeny for dna gyrase subunit b (gyrb), rna-polymerase subunit b (rpob), recombinase a (reca), and polyphosphate kinase (ppk). the resulting gene trees were compared with the 16s rrna gene phylogeny of the same strains. the topology of neighbour-joining and maximum parsimony phylogenetic trees, based on nucleic-acid sequences and protein sequences of housekeeping genes, differ ... | 2007 | 17551787 |
| effects of ultraviolet radiation on the gram-positive marine bacterium microbacterium maritypicum. | although extensive information is available on the effect ultraviolet (uv) radiation has on gram-negative marine bacteria, there is a scarcity of data concerning uv radiation and gram-positive marine bacteria. the focus of this paper is on microbacterium maritypicum, with the gram-negative vibrio natriegens being used as a standard of comparison. m. maritypicum exhibited growth over a nacl range of 0-1000 mm: , with optimum growth occurring between 0 and 400 mm: nacl. in contrast, v. natriegens ... | 2007 | 17551790 |
| enhanced removal of dimethyl sulfide from a synthetic waste gas stream using a bioreactor inoculated with microbacterium sp. ntut26 and pseudomonas putida. | the removal of dimethyl sulfide (dms) from industrial gas streams has received a high priority due to its very low odorous threshold value and relatively low biodegradability compared to other reduced sulfur compounds. a variety of bacteria that utilize dms as a carbon/energy source have been studied and the degradation pathway elucidated. however, to date, there have been few reports on the industrial application of such bacteria inoculated into a bioreactor for dms treatment. an additional pro ... | 2009 | 18815822 |