Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| a novel dps-type protein from insect gut bacteria catalyses hydrolysis and synthesis of n-acyl amino acids. | a novel type of a microbial n-acyl amino acid hydrolase (aah) from insect gut bacteria was purified, cloned and functionally characterized. the enzyme was obtained from microbacterium arborescens se14 isolated from the foregut of larvae of the generalist herbivore spodoptera exigua. the substrates of aah are n-acyl-glutamines previously reported to elicit plant defence reactions after introduction into the leaf during feeding. the isolated aah catalyses the hydrolysis of the amide bond (k(m) = 3 ... | 2007 | 17504494 |
| antimicrobial properties of two purified skin peptides from the mink frog (rana septentrionalis) against bacteria isolated from the natural habitat. | numerous peptides exhibiting antimicrobial properties have been isolated from the skins of many amphibian species. these peptides offer an innate chemical defense system against various microbial agents that exist in the amphibian's environment. amphibian skin peptides are typically tested for antimicrobial activity against microbial strains that are pathogenic to humans, but not on potential pathogenic or opportunistic bacteria that exist in the organism's habitat. two peptides, a brevinin-2-re ... | 2007 | 17499556 |
| screening and distributing features of bacteria with hydantoinase and carbamoylase. | hydantoinase and carbamoylase are key biocatalysts for the production of optically pure amino acids from dl-5-substituted hydantoins (ssh). out of 364 isolated strains with hydantoinase and carbamoylase at 45 degrees c, 24 strains showed efficient hydantoinase and carbamoylase activities. among them, 17 produced l-amino acids, and 7 produced d-amino acids from both aromatic dl-5-benzylhydantoin and aliphatic dl-5-isopropylhydantoin. most of the strains that were able to form l-amino acid belonge ... | 2009 | 17498938 |
| isolation and characterization of a xanthan-degrading microbacterium sp. strain xt11 from garden soil. | isolation and characterization of the xanthan-degrading microbacterium sp. xt11. | 2007 | 17448171 |
| stability of the biodiversity of the surface consortia of gubbeen, a red-smear cheese. | a total of 1,052 bacteria and 828 yeasts were isolated from the surface flora of 6 batches of gubbeen cheese made in 1996-1997 and 2002-2003. stability of the microflora was evaluated over time and also during ripening at 4, 10, and 16 d (batches 4, 5, and 6) or at 4, 16, 23, and 37 d (batches 1, 2, and 3). bacteria were identified using pulsed-field gel electrophoresis, repetitive extragenic palindromic-pcr, and 16s rrna gene sequencing, and yeasts were identified by fourier transform infrared ... | 2007 | 17430918 |
| microbacterium terricolae sp. nov., isolated from soil in japan. | the taxonomic positions of two novel strains isolated from a soil sample collected in japan using glucose-peptone-meat extract (gpm) agar plates supplemented with superoxide dismutase or superoxide dismutase plus catalase were investigated based on the results of chemotaxonomic, phenotypic and genotypic characteristics. strains were gram-positive, catalase-positive, non-motile bacteria with l-ornithine as a diagnostic diamino acid of the peptidoglycan. the acyl type of the peptidoglycan was n-gl ... | 2007 | 17429156 |
| isolation and identification of potential phosphate solubilizing bacteria from the rhizoplane of oryza sativa l. cv. br29 of bangladesh. | a total of 30 bacteria were isolated from the rhizoplane of rice cv. br29 cultivated in mymensingh, bangladesh and from the seedlings obtained from surface-sterilized seeds of br29. upon screening, 6 isolates showed varying levels of phosphate solubilizing activity in both agar plate and broth assays using national botanical research institute's phosphate medium. the bacterial isolates were identified based on their phenotypic and 16s rrna genes sequencing data as acinetobacter sp. br-12, klebsi ... | 2007 | 17425114 |
| culture-independent analysis of bacterial diversity in a child-care facility. | child-care facilities appear to provide daily opportunities for exposure and transmission of bacteria and viruses. however, almost nothing is known about the diversity of microbial contamination in daycare facilities or its public health implications. recent culture-independent molecular studies of bacterial diversity in indoor environments have revealed an astonishing diversity of microorganisms, including opportunistic pathogens and many uncultured bacteria. in this study, we used culture and ... | 2007 | 17411442 |
| [reaction of microorganisms to the digestive fluid of the earthworms]. | the reaction of soil bacteria and fungi to the digestive fluid of the earthworm aporrectodea caliginosa was studied. the fluid was obtained by centrifugation of the native enzymes of the digestive tract. the inhibition of growth of certain bacteria, spores, and fungal hyphae under the effect of extracts from the anterior and middle sections of the digestive tract of a. caliginosa was discovered for the first time. in bacteria, microcolony formation was inhibited as early as 20-30 s after the app ... | 2007 | 17410875 |
| metabolic activity and phylogenetic diversity of reed (phragmites australis) periphyton bacterial communities in a hungarian shallow soda lake. | in the present study, the species composition and potential metabolic activities of bacterial communities of reed phragmites australis (cav.) (trin. ex steudel) periphyton from lake velencei were studied by cultivation-based and metabolic fingerprinting methods. serially diluted spring biofilm samples were used to test the community-level physiological profiling (clpp) using biolog microplates, and for plating onto different media. on the basis of their morphological, biochemical, and physiologi ... | 2007 | 17406774 |
| microbacterium isolates from the vicinity of a radioactive waste depository and their interactions with uranium. | three oligotrophic bacterial strains were cultured from the ground water of the deep-well monitoring site s15 of the siberian radioactive waste depository tomsk-7, russia. they were affiliated with actinobacteria from the genus microbacterium. the almost fully sequenced 16s rrna genes of two of the isolates, s15-m2 and s15-m5, were identical to those of cultured representatives of the species microbacterium oxydans. the third isolate, s15-m4, shared 99.8% of 16s rrna gene identity with them. the ... | 2007 | 17381522 |
| [evaluation of api coryne system, version 2.0, for diphteroid gram-positive rods identification with clinical relevance]. | the ability of the api coryne system, version 2.0, to identify 178 strains of gram-positive rods was evaluated. seventy eight isolates belonged to genus corynebacterium and one hundred to related genera, all strains were isolated from clinical samples at the laboratory of bacteriology, hospital de clínicas josé de san martin (uba) between 1995 and 2004. the isolates were identified according to von graevenitz and funke's scheme. one hundred and sixty two out of 178 strains (91%) were correctly i ... | 2006 | 17370571 |
| caenorhabditis elegans mutants resistant to attachment of yersinia biofilms. | the detailed composition and structure of the caenorhabditis elegans surface are unknown. previous genetic studies used antibody or lectin binding to identify srf genes that play roles in surface determination. infection by microbacterium nematophilum identified bus (bacterially unswollen) genes that also affect surface characteristics. we report that biofilms produced by yersinia pestis and y. pseudotuberculosis, which bind the c. elegans surface predominantly on the head, can be used to identi ... | 2007 | 17339204 |
| 17beta-estradiol-degrading bacteria isolated from activated sludge. | fourteen phylogenetically diverse 17beta-estradiol-degrading bacteria (strains kc1-14) were isolated from activated sludge of a wastewater treatment plant. these isolates widely distributed among eight different genera--aminobacter (strains kc6 and kc7), brevundimonas (strain kc12), escherichia (strain kc13), flavobacterium (strain kc1), microbacterium (strain kc5), nocardioides (strain kc3), rhodococcus (strain kc4), and sphingomonas (strains kc8-kc11 and kc14)--of three phyla: proteobacteria, ... | 2007 | 17310711 |
| heavy metal resistance and genotypic analysis of metal resistance genes in gram-positive and gram-negative bacteria present in ni-rich serpentine soil and in the rhizosphere of alyssum murale. | forty-six bacterial cultures, including one culture collection strain, thirty from the rhizosphere of alyssum murale and fifteen from ni-rich soil, were tested for their ability to tolerate arsenate, cadmium, chromium, zinc, mercury, lead, cobalt, copper, and nickel in their growth medium. the resistance patterns, expressed as minimum inhibitory concentrations, for all cultures to the nine different metal ions were surveyed by using the agar dilution method. a large number of the cultures were r ... | 2007 | 17276484 |
| identification of diazotrophs in the culturable bacterial community associated with roots of lasiurus sindicus, a perennial grass of thar desert, india. | lasiurus sindicus is a highly nutritive, drought-tolerant, perennial grass that is endemic to the thar desert of rajasthan, india. analysis of 16s rrna coding genes of the bacterial isolates enriched in nitrogen-free semisolid medium, from the surface-sterilized roots of l. sindicus, showed predominance of gram-negative over gram-positive bacteria. according to comparative sequence analysis of 16s rdna sequence data, gram-positive bacteria with low gc content (staphylococcus warneri and bacillus ... | 2007 | 17264993 |
| a new microbacterium species isolated from the blood of a patient with fever: microbacterium pyrexiae sp. nov. | a gram-positive bacterium, smc-a8265(t), which was isolated from the blood of a patient with fever but could not be identified by a conventional microbiologic method, was finally characterized by performing phenotypic and genotypic analyses. 16s rrna gene sequence analysis revealed that the strain smc-a8265(t) belonged to the genus microbacterium, but it did not correspond to any of the previously described microbacterium spp. biochemical tests and cellular fatty acid composition analysis also c ... | 2007 | 17240108 |
| chilling and cultivar type affect the diversity of bacterial endophytes colonizing sweet pepper (capsicum anuum l.). | a climate chamber experiment was conducted to assay the effect of low temperatures (chilling) on the diversity of bacteria colonizing the endospheres of two thermophilic sweet pepper (capsicum anuum l.) cultivars, milder spiral and ziegenhorn bello. structural diversity was analyzed by 16s rrna-based terminal restriction fragment length polymorphism (t-rflp) analysis and by the generation of 16s rrna gene libraries to determine dominant community members in t-rflp profiles. cultivable community ... | 2006 | 17215894 |
| characterization of extracellular polymers synthesized by tropical intertidal biofilm bacteria. | this study was performed to determine the potential of tropical intertidal biofilm bacteria as a source of novel exopolymers (eps). | 2007 | 17184342 |
| mos1 mutagenesis reveals a diversity of mechanisms affecting response of caenorhabditis elegans to the bacterial pathogen microbacterium nematophilum. | a specific host-pathogen interaction exists between caenorhabditis elegans and the gram-positive bacterium microbacterium nematophilum. this bacterium is able to colonize the rectum of susceptible worms and induces a defensive tail-swelling response in the host. previous mutant screens have identified multiple loci that affect this interaction. some of these loci correspond to known genes, but many bus genes [those with a bacterially unswollen (bus) mutant phenotype] have yet to be cloned. we em ... | 2007 | 17151260 |
| molecular diversity studies of bacterial communities of oil polluted microbial mats from the etang de berre (france). | the biodiversity of microbial mats inhabiting the oil-contaminated lagoon etang de berre was determined by molecular approaches. the fingerprint of denaturing gradient gel electrophoresis (dgge) and automatic ribosomal intergenic spacer analysis (arisa) of mats exposed to different pollution levels showed specific microbial communities for each site but similar diversity richness. species composition of the mats were compared by constructing 16s rrna libraries. amplified rdna restriction analysi ... | 2006 | 17117996 |
| activities and survival of endophytic bacteria in white clover (trifolium repens l.). | in this study, the genera, abundance, and activities of endophytic bacteria in field-grown white clover (trifolium repens) and the fate of introduced antibiotic-tolerant bacteria in white clover tissues were investigated. pseudomonas, pantoea, and corynebacterium were the most frequently isolated endophytic bacteria genera, whereas xanthomonas, microbacterium, and cellulomonas occurred less frequently. the average bacterial populations in stolons and roots were approximately 100,000 colony-formi ... | 2006 | 17110977 |
| purification and properties of a keratinolytic metalloprotease from microbacterium sp. | this study was developed to purify and to characterize a keratinolytic protease from the bacterium microbacterium sp. strain kr10. | 2006 | 17105556 |
| degradation of environmental endocrine disruptor di-2-ethylhexyl phthalate by a newly discovered bacterium, microbacterium sp. strain cq0110y. | in this study di-2-ethylhexyl phthalate (dehp)-degradation strain cq0110y was isolated from activated sludge. according to the biophysical/biochemical characteristics and analysis of 16s rdna, the strain was identified as microbacterium sp. the results of this study showed the optimal ph value and optimal temperature which influenced the degradation rate in wastewater: ph 6.5-7.5, 25-35 degrees c. kinetics of degradation reaction had been performed at different initial concentrations and differe ... | 2007 | 17089119 |
| genome characterization, analysis of virulence and transformation of microbacterium nematophilum, a coryneform pathogen of the nematode caenorhabditis elegans. | a coryneform bacterium designated microbacterium nematophilum has previously been reported to act as a pathogen for caenorhabditis elegans. this bacterium is able to colonize the rectum of infected worms and cause localized swelling, constipation and slowed growth. additional isolates and analysis of this bacterium are described here. tests of pathogenicity on other caenorhabditis nematodes show that m. nematophilum infection is lethal to most species in the genus, in contrast to its relatively ... | 2006 | 17010162 |
| growth and colour development of some surface ripening bacteria with debaryomyces hansenii on aseptic cheese curd. | the growth of five bacteria isolated from red-smear cheeses, brevibacterium aurantiacum, corynebacterium casei, corynebacterium variabile, microbacterium gubbeenense and staphylococcus saprophyticus in mixed cultures with debaryomyces hansenii on aseptic model cheese curd at 10 and 14 degrees c was investigated. at both temperatures, c. casei and micro. gubbeenense had a longer lag phase than c. variabile, brevi. aurantiacum and staph. saprophyticus. in all cultures, lactose was utilised first a ... | 2006 | 16978429 |
| microbacterium deminutum sp. nov., microbacterium pumilum sp. nov. and microbacterium aoyamense sp. nov. | three novel bacterial strains were isolated from a soil sample collected in japan by culture on a gpm agar plate supplemented with superoxide dismutase and catalase. the strains were gram-positive, catalase-positive, non-motile bacteria with l-ornithine as a diagnostic diamino acid of the peptidoglycan. the acyl type of the peptidoglycan was n-glycolyl. the major menaquinones were mk-12, 13 and 14. mycolic acids were not detected. g+c contents of the dna were in the range 69-71 mol%. comparative ... | 2006 | 16957107 |
| substrate induction and statistical optimization for the production of chitosanase from microbacterium sp. ou01. | the chitosanase production was markedly enhanced by substrate induction, statistical optimization of medium composition and culture conditions by microbacterium sp. ou01 in shake-flask. a significant influence of (nh(4))(2)so(4), mgso(4).7h(2)o and initial ph on chitosanase production was noted with plackett-burman design. it was then revealed with the method of steepest ascent and response surface methodology (rsm) that 19.0g/l (nh(4))(2)so(4), 1.3g/l mgso(4) and an initial ph of 2.0 were optim ... | 2007 | 16930999 |
| purification and characterization of two types of chitosanase from a microbacterium sp. | two extracellular chitosanases (chix and chin) were extracted from microbacterium sp. ou01 with mr values of 81 kda (chix) and 30 kda (chin). chin was optimally active at ph 6.2 and 50 degrees c and chix at ph 6.6 and 60 degrees c (assayed over 15 min). both the activities increased with the degree of deacetylation (dda) of chitosan. chin hydrolyzed oligomers of glucosamine (glcn) larger than chitopentaose, and chitosan with 62-100% dda; but chix acted on chitosan and released glcn. hydrolysis o ... | 2006 | 16820974 |
| genomic clusters, putative pathogen recognition molecules, and antimicrobial genes are induced by infection of c. elegans with m. nematophilum. | the interaction between the nematode caenorhabditis elegans and a gram-positive bacterial pathogen, microbacterium nematophilum, provides a model for an innate immune response in nematodes. this pathogen adheres to the rectal and post-anal cuticle of the worm, causing slowed growth, constipation, and a defensive swelling response of rectal hypodermal cells. to explore the genomic responses that the worm activates after pathogenic attack we used microarray analysis of transcriptional changes indu ... | 2006 | 16809667 |
| [effect of pentachlorophenol (pcp) on bacterial community of an aerobic granular sludge estimated by t-rflp]. | effect of pentachlorophenol (pcp) on cod and ammonium removal rates was investigated, and also changes of bacterial community within aerobic granular sludge were monitored by terminal restriction fragment length polymorphism (t-rflp) technique. compared with the removal rate of cod, the rate of ammonium removal is more sensitive to pcp. as the ammonium and cod removal rates decreased consistently, the bacterial genera within the aerobic granular sludge also decrease with the increase of pcp conc ... | 2006 | 16768009 |
| enhanced biofilm formation and increased resistance to antimicrobial agents and bacterial invasion are caused by synergistic interactions in multispecies biofilms. | most biofilms in their natural environments are likely to consist of consortia of species that influence each other in synergistic and antagonistic manners. however, few reports specifically address interactions within multispecies biofilms. in this study, 17 epiphytic bacterial strains, isolated from the surface of the marine alga ulva australis, were screened for synergistic interactions within biofilms when present together in different combinations. four isolates, microbacterium phyllosphaer ... | 2006 | 16751497 |
| the phylogenetic significance of peptidoglycan types: molecular analysis of the genera microbacterium and aureobacterium based upon sequence comparison of gyrb, rpob, reca and ppk and 16srrna genes. | the type strains of 27 species of the genus microbacterium, family microbacteriaceae, were analyzed with respect to the phylogeny of the housekeeping genes coding for dna gyrase subunit b (gyrb), rna-polymerase subunit b (rpob), recombinase a (reca) and polyphosphate kinase (ppk). the resulting gene trees were compared to the 16s rrna gene phylogeny of the same species. the topology of neighbour-joining and maximum parsimony phylogenetic trees based upon nucleic acid sequences and protein sequen ... | 2007 | 16684595 |
| rapid analysis of two food-borne microbial communities at the species level by fourier-transform infrared microspectroscopy. | the species composition of microbial communities in natural habitats may be extremely complex and therefore a quantitative analysis of the fraction each species contributes to the consortium has proven to be difficult. during recent years, the identification of bacterial pure cultures based on their infrared spectra has been established. fourier-transform infrared microspectroscopy now proceeds a step further and allows identification of microorganisms directly plated from community dilutions. i ... | 2006 | 16623742 |
| screening for microbial markers in miocene sediment exposed during open-cast brown coal mining. | viable microorganisms were found in miocene lacustrine clays of the cypris formation excavated from 200-m below the surface as spoil during open-cast brown coal mining (sokolov brown coal basin, north-western bohemia, czech republic). both saprotrophic microfungi of the genera penicillium, verticillium, cladosporium and aspergillus as well as heterotrophic bacteria were isolated from an intact sediment cores. heterotrophic bacteria were classified by the mis sherlock system as representatives of ... | 2006 | 16622790 |
| the hydantoin transport protein from microbacterium liquefaciens. | the gene hyup from microbacterium liquefaciens aj 3912 with an added his6 tag was cloned into the expression plasmid pttq18 in an escherichia coli host strain. the transformed e. coli showed transport of radioisotope-labeled 5-substituted hydantoins with apparent k(m) values in the micromolar range. this activity exhibited a ph optimum of 6.6 and was inhibited by dinitrophenol, indicating the requirement of energy for the transport system. 5-indolyl methyl hydantoin and 5-benzyl hydantoin were t ... | 2006 | 16621827 |
| survival of surface ripening cultures during storage and monitoring their development on cheese. | to study the survival of bacteria isolated from the surface of smear cheese and monitor their development during cheese ripening. | 2006 | 16599999 |
| diverse bacteria associated with root nodules of spontaneous legumes in tunisia and first report for nifh-like gene within the genera microbacterium and starkeya. | we characterized 34 endophytic bacterial isolates associated to root nodules collected from spontaneous legumes in the arid zone of tunisia by 16s rdna polymerase chain reaction (pcr)-restriction fragment length polymorphism, whole cell protein sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page), 16s rdna and 16s-23s rdna internal transcribed spacer sequencing. phylogenetically, these isolates belong to the branches containing the genera inquilinus, bosea, rhodopseudomonas, para ... | 2006 | 16598639 |
| planktonic replication is essential for biofilm formation by legionella pneumophila in a complex medium under static and dynamic flow conditions. | legionella pneumophila persists for a long time in aquatic habitats, where the bacteria associate with biofilms and replicate within protozoan predators. while l. pneumophila serves as a paradigm for intracellular growth within protozoa, it is less clear whether the bacteria form or replicate within biofilms in the absence of protozoa. in this study, we analyzed surface adherence of and biofilm formation by l. pneumophila in a rich medium that supported axenic replication. biofilm formation by t ... | 2006 | 16597995 |
| the microbacteria ii. : the growth of microbacterium lacticum in a chemically defined medium. | 1949 | 16561650 | |
| microbacterium paludicola sp. nov., a novel xylanolytic bacterium isolated from swamp forest. | a xylanolytic bacterium, us15t, was isolated from swamp forest soil in ulsan, korea. the cells of the novel strain were gram-positive, non-motile, short-rod-shaped and showed chemotaxonomic properties that were consistent with its classification in the genus microbacterium. chemotaxonomic results showed mk-12 and mk-11 as major menaquinones, predominating iso- and anteiso-branched cellular fatty acids, glucose, galactose and mannose as cell-wall sugars, peptidoglycan-type b2beta with glycolyl re ... | 2006 | 16514023 |
| identifying the bacterial community on the surface of intralox belting in a meat boning room by culture-dependent and culture-independent 16s rdna sequence analysis. | we examined the bacterial community present on an intralox conveyor belt system in an operating lamb boning room by sequencing the 16s ribosomal dna (rdna) of bacteria extracted in the presence or absence of cultivation. rflp patterns for 16s rdna clone library and cultures were generated using haeiii and mspi restriction endonucleases. 16s rdna amplicons produced 8 distinct rflp pattern groups. rflp groups i-iv were represented in the clone library and rflp groups i and v-viii were represented ... | 2006 | 16488497 |
| interstitial pulmonary inflammation due to microbacterium sp. after heart transplantation. | a coryneform bacterium was isolated from the bronchoalveolar aspirate of a patient with interstitial pulmonary inflammation. commercial systems identified the isolate as corynebacterium sp. or aureobacterium sp./corynebacterium aquaticum, but 16s rrna gene analysis unequivocally attributed it to the genus microbacterium. this represents the first documented case of microbacterium pulmonary infection. | 2006 | 16476799 |
| microbacterium koreense sp. nov., from sea water in the south sea of korea. | microbacterium strains js53-2t and js53-5 were isolated from sea water in the south sea of korea and subjected to phenotypic, chemotaxonomic and genetic characterization. the cells were found to be gram-positive. these strains contained mk-11 and mk-12 as the main respiratory quinones and anteiso-c15:0, anteiso-c17:0 and iso-c16:0 as the major cellular fatty acids. the dna g+c content was 68 mol%. the cell-wall sugars of the isolates were galactose and xylose, and the diamino acid in the cell-wa ... | 2006 | 16449451 |
| bacteria isolated from the different developmental stages and larval organs of the obligate parasitic fly, wohlfahrtia magnifica (diptera: sarcophagidae). | wohlfahrtia magnifica (diptera: sarcophagidae) is the major myiasis-causing fly species in the whole of eurasia for most important domestic animals. the aim of the present work was to obtain data on the culturable bacteria isolated under aerobic conditions from this fly: bacteria were isolated from all developmental stages (larvae, pupa, and imago) of wohlfahrtia magnifica, and the third-stage larval organs were also sampled. to determine the possible antagonistic effects between the dominant ba ... | 2006 | 16382282 |
| glycerol ester hydrolase activity of microbacterium thermosphactum. | microbacterium thermosphactum possesses a significant glycerol ester hydrolase (lipase, ec 3.1.1.3) activity and a weak but definite carboxylic ester hydrolase (esterase, ec 3.1.1.1) activity. harvested whole cell preparations contained 53 units of lipase activity with tripropionin as the substrate. this activity decreased with an increasing chain length of fatty acid in the triglyceride to 13 units with trilaurin as the substrate and no activity with tripalmitin. maximum lipase activity was fou ... | 1971 | 16349896 |
| effect of gamma irradiation on the microflora of freshwater fish: ii. generic identification of aerobic bacteria from yellow perch fillets. | studies on the generic identification of bacteria isolated from nonirradiated and irradiated (0.3 and 0.6 mrad) yellow perch fillets during the course of microbial spoilage have been conducted. after the enumeration and tabulation of macrocolonies on petri dish cultures obtained from fillets, isolates were examined and keyed out essentially according to modified morphological and biochemical protocols of shewan. identification was further confirmed through reference to bergey's manual. data obta ... | 1966 | 16349702 |
| effect of carbon dioxide on growth of meat spoilage bacteria. | the ability of co(2) to inhibit respiration and growth of representative strains of seven species of meat spoilage bacteria was examined. enterobacter and microbacterium thermosphactum were unaffected by co(2). both respiration and growth of the other species were inhibited. with four of the species (fluorescent and nonfluorescent pseudomonas, alteromonas putrefaciens, and yersinia enterocolitica), the inhibition pattern in a complex medium was similar, and inhibition was incomplete and reached ... | 1980 | 16345503 |
| nutritional requirements of microbacterium thermosphactum. | microbacterium thermosphactum requires cysteine, alpha-lipoate, nicotinate, pantothenate, p-aminobenzoate, biotin, and thiamin for aerobic growth in glucose-mineral salts medium. glucose cannot be replaced by casamino acids or acids of the tricarboxylic acid cycle as sole carbon and energy sources. the organism can also grow anaerobically in the minimal synthetic medium. | 1979 | 16345460 |
| symbiotic association in chlorella culture. | chlorella sorokiniana iam c-212 has long been maintained in slant culture as a mixed strain, representing an associated natural microbial consortium. in this study, the consortium was separated and five nonalgal constituents, a fungal strain (cssf-1), and four bacterial strains (cssb-1, cssb-2, cssb-3, and cssb-4) were isolated and identified. 16s rdna sequence analysis revealed that strains cssb-1, cssb-2, cssb-3, and cssb-4 were close to ralstonia pickettii (99.8% identity), sphingomonas sp. d ... | 2005 | 16329867 |
| characterization of the novel hch-degrading strain, microbacterium sp. itrc1. | a gram-positive microbacterium sp. strain, itrc1, that was able to degrade the persistent and toxic hexachlorocyclohexane (hch) isomers was isolated and characterized. the itrc1 strain has the capacity to degrade all four major isomers of hch present in both liquid cultures and aged contaminated soil. dna fragments corresponding to the two initial genes involved in gamma-hch degradative pathway, encoding enzymes for gamma-pentachlorocyclohexene hydrolytic dehalogenase (linb) and a 2,5-dichloro-2 ... | 2006 | 16315057 |
| surface microflora of four smear-ripened cheeses. | the microbial composition of smear-ripened cheeses is not very clear. a total of 194 bacterial isolates and 187 yeast isolates from the surfaces of four irish farmhouse smear-ripened cheeses were identified at the midpoint of ripening using pulsed-field gel electrophoresis (pfge), repetitive sequence-based pcr, and 16s rrna gene sequencing for identifying and typing the bacteria and fourier transform infrared spectroscopy and mitochondrial dna restriction fragment length polymorphism (mtdna rflp ... | 2005 | 16269673 |
| biofilm formation characteristics of bacterial isolates retrieved from a reverse osmosis membrane. | high-quality water purification systems using reverse osmosis (ro) membrane separation have faced a major challenge related to biofilm formation on the membrane surface, or biofouling. to understand this issue, the biofilm formation characteristics of four bacterial isolates previously retrieved from an ro membrane treating potable water were investigated. biofilm formation of all four isolates occurred to different extents in microtiter plates and could be related to one or more cell properties ... | 2005 | 16245826 |
| in vitro influence of bacterial mixtures on fusarium verticillioides growth and fumonisin b1 production: effect of seeds treatment on maize root colonization. | enterobacter cloacae, microbacterium oleovorans, pseudomonas solanacearum and bacillus subtilis were investigated in order to evaluate: (i) the inoculum size of two bacterial mixtures on fusarium verticillioides growth and fumonisin b1 production in vitro at different water activities and (ii) the efficacy of a seed treatment with the best bacterial mixture on f. verticillioides root colonization in greenhouse studies. | 2005 | 16238641 |
| coaggregation between acinetobacter johnsonii s35 and microbacterium esteraromaticum strains isolated from sewage activated sludge. | the extent and nature of intergeneric coaggregations among non-flocculating sludge bacteria were studied through examination of the coaggregation abilities of acinetobacter johnsonii s35 with two other strains of non-flocculating sludge bacteria (microbacterium esteraromaticum s38 and m. esteraromaticum s51). at first, the effect of electrolyte concentration as well as the addition of edta and proteases on coaggregation were studied. changes in electrolyte concentration had little effect on the ... | 2003 | 16233476 |
| glyceroglycolipids preventing tert-butylhydroperoxide-induced cell death from microbacterium sp. and corynebacterium aquaticum strains. | galactosyl diacylglycerols m874b and s365b obtained from the recently isolated bacteria identified as microbacterium sp. m874 and corynebacterium aquaticum s365 were found to prevent oxidative cell death induced by tert-butylhydroperoxide. their structures were determined to be 1,2-di-o-(12-methyltetradecanoyl)-3-o-beta-d-galactopyranosyl-sn-glycerol and 1-o-(14-methylhexadecanoyl)-2-o-(12-methyltetradecanoyl)-3-o-beta-d-galactopyranosyl-sn-glycerol, respectively. | 2000 | 16232721 |
| the selection of mixed microbial inocula in environmental biotechnology: example using petroleum contaminated tropical soils. | the impact of inorganic n and p additions on a tropical soil contaminated with petroleum hydrocarbons was investigated using molecular and culture techniques. microcosms were incubated for 42 days and sampled at 0, 1, 7, 28 and 42 days. changes in bacterial community structure were determined using denaturing gradient gel electrophoresis (dgge) of the rrna following reverse transcription pcr using primers specific to the v3 region of the 16s rrna gene. to identify which components of the microbi ... | 2006 | 16226327 |
| microbacterium xylanilyticum sp. nov., a xylan-degrading bacterium isolated from a biofilm. | a novel xylan-degrading bacterium, s3-e(t), was isolated from the biofilm of a membrane bioreactor. the cells of this strain were gram-positive, non-motile, non-spore-forming rods, produced primary branches and formed yellow colonies on nutrient agar. the strain had chemotaxonomic markers that were consistent with classification in the genus microbacterium, i.e. mk-12, mk-11 and mk-13 as the major menaquinones, predominant iso- and anteiso-branched cellular fatty acids, glucose and galactose as ... | 2005 | 16166712 |
| synthesis and characterisation of a ligand that forms a stable tetrahedral intermediate in the active site of the aureobacterium species (-) gamma-lactamase. | the crystal structure of a (-) gamma-lactamase from an aureobacterium species showed a molecule bound covalently to the active site serine residue. this enzyme complex represented the first structure of a stably bound tetrahedral intermediate for an alpha/beta hydrolase fold enzyme. the structural elucidation of tetrahedral intermediates is important for the understanding of enzymatic mechanism, substrate recognition and enzyme inhibition. in this paper, we report the synthesis and subsequent ch ... | 2005 | 16132085 |
| [isoprenoid pigments in representatives of the family microbacteriaceae]. | by using fosmidomycin and mevinolin (inhibitors of the synthesis of isoprenoid pigments), spectrophotometry, and mass spectrometry, the presence of isoprenoid pigments is shown in 71 of the 78 strains under study. all of these strains belong to 11 genera of the family microbacteriaceae. yellow, orange, and red pigments are found to have absorption spectra typical of c40-carotenoids. eight out of the sixteen strains of the genus microbacterium are able to synthesize neurosporene, a precursor of l ... | 2005 | 16119846 |
| molecular cloning and expression of the hyu genes from microbacterium liquefaciens aj 3912, responsible for the conversion of 5-substituted hydantoins to alpha-amino acids, in escherichia coli. | a dna fragment from microbacterium liquefaciens aj 3912, containing the genes responsible for the conversion of 5-substituted-hydantoins to alpha-amino acids, was cloned in escherichia coli and sequenced. seven open reading frames (hyup, hyua, hyuh, hyuc, orf1, orf2, and orf3) were identified on the 7.5 kb fragment. the deduced amino acid sequence encoded by the hyua gene included the n-terminal amino acid sequence of the hydantoin racemase from m. liquefaciens aj 3912. the hyua, hyuh, and hyuc ... | 2005 | 16116274 |
| evaluation of microbial strains for linoleic acid hydroxylation and reclassification of strain ala2. | in previous studies, a new microbial strain ala2 was isolated which produced many new products from linoleic acid [gardner h.w., hou c.t., weisleder d. and brown w. 2000. lipids 35: 1055-1060; hou c.t. 1998. 12,13,17-trihydroxy-9(z)-octodecenoic acid and derivatives and microbial isolate for production of the acid. us patent no. 5, 852, 196]. strain ala2 was preliminary identified as clavibacter sp. based on its physiological and fatty acid profiles. to determine if strain ala2 is the optimal st ... | 2005 | 16096693 |
| multiple genes affect sensitivity of caenorhabditis elegans to the bacterial pathogen microbacterium nematophilum. | interactions with bacteria play a major role in immune responses, ecology, and evolution of all animals, but they have been neglected until recently in the case of c. elegans. we report a genetic investigation of the interaction of c. elegans with the nematode-specific pathogen microbacterium nematophilum, which colonizes the rectum and causes distinctive tail swelling in its host. a total of 121 mutants with altered response to infection were isolated from selections or screens for a bacteriall ... | 2005 | 16079230 |
| active membrane transport and receptor proteins from bacteria. | a general strategy for the expression of bacterial membrane transport and receptor genes in escherichia coli is described. expression is amplified so that the encoded proteins comprise 5-35% of e. coli inner membrane protein. depending upon their topology, proteins are produced with rgsh6 or a strep tag at the c-terminus. these enable purification in mg quantities for crystallization and nmr studies. examples of one nutrient uptake and one multidrug extrusion protein from helicobacter pylori are ... | 2005 | 16042616 |
| [bacterial conversion of sulfur-and phosphorous-compounds and microbial diversity in sediments from a near-shore marine-cultural region]. | the h2s-producing bacteria and the phosphorous-cycling bacteria in sediments from near-shore marine-cultural region were investigated. results indicate that the bacterial h2s production in aerobic condition is the dominating process to produce h2s in the sediment of cultural pond. the total counts of h2s-producing bacteria utilizing cysteine and na2s2o3 were 1.6 x 10(6) and 4.35 x 10(3) cells g(-1) respectively. the counts of sulfate-reducing bacteria in the sediments were very little, only 2.5 ... | 2005 | 16004320 |
| biodesulfurization of dibenzothiophene and other organic sulfur compounds by a newly isolated microbacterium strain zd-m2. | a new bacterial strain capable of selectively desulfurizing dibenzothiphene (dbt) was isolated from sludge. this organism, designated zd-m2, clustered most closely with members of the genus microbacterium, as determined by 16s rrna gene sequence analysis. metabolites produced by dbt desulfurization were identified by gc-ms, and two sulfur-free products, 2-methoxybiphenyl (2-mbp) and biphenyl, were detected in addition to 2-hydroxybiphenyl (2-hbp). this strain can also totally degrade 4,6-dimethy ... | 2005 | 15927746 |
| purification and properties of a new type of protease produced by microbacterium liquefaciens. | a bacterium, identified as microbacterium liquefaciens mim-cg-9535-i, was isolated from a soil sample taken from the industrial site of a gelatin manufacturer. a new type of protease, which restrictively decomposes gelatin at one or two positions, was purified from the bacterial culture. the molecular mass of the purified enzyme was 21 kda by sds-polyacrylamide gel electrophoresis. the purified enzyme specifically degraded the alpha-chain of gelatin with a molecular weight of 100 kda into two pe ... | 2005 | 15914910 |
| [auxin production by bacteria associated with orchid roots]. | bacteria associated with the roots of greenhouse tropical orchids were shown to produce indole-3-acetic acid (iaa) and to excrete it into the culture liquid. the presence and activity of iaa were demonstrated colorimetrically, by thin-layer chromatography, and by biotests. the associated bacteria varied in their ability to excrete indole compounds (1-28 microg/ml nutrient broth). addition of tryptophan to the growth medium enhanced phytohormone production. upon addition of 200 microg/ml tryptoph ... | 2005 | 15835779 |
| reduction of cr(vi) by immobilized cells of desulfovibrio vulgaris ncimb 8303 and microbacterium sp. ncimb 13776. | hexavalent chromium, a carcinogen and mutagen, can be reduced to cr(iii) by desulfovibrio vulgaris ncimb 8303 and microbacterium sp. ncimb 13776. this study examined cr(vi) reduction by immobilized cells of the two strains in a common solution matrix using various entrapment matrices. chitosan and pva-borate beads did not retain integrity and supported low or no reduction of cr(vi) by the cells. a commercial preparation (lentikats) was stable but also did not support cr(vi) reduction. k-carragee ... | 2005 | 15818565 |
| characterisation of the nitrile hydratase gene clusters of rhodococcus erythropolis strains aj270 and aj300 and microbacterium sp. aj115 indicates horizontal gene transfer and reveals an insertion of is1166. | the nitrile metabolising strains aj270, aj300 and aj115 were isolated from the same location. the strains have very similar nitrile metabolising profiles. sequencing of the 16s rrna gene indicates that strains aj270 and aj300 are novel strains of rhodococcus erythropolis while strain aj115 is a novel microbacterium strain very closely related to microbacterium oxydans and microbacterium liquefaciens. analysis of the structure of the nitrile hydratase/amidase gene clusters in the three strains in ... | 2005 | 15803388 |
| purification and characterization of hydantoin racemase from microbacterium liquefaciens aj 3912. | a hydantoin racemase that catalyzed the racemization of 5-benzyl-hydantoin was detected in a cell-free extract of microbacterium liquefaciens aj 3912, a bacterial strain known to produce l-amino acids from their corresponding dl-5-substituted-hydantoins. this hydantoin racemase was purified 658-fold to electrophoretic homogeneity by serial chromatography. the n-terminal amino acid sequence of the enzyme showed homology with known hydantoin racemases from other microorganisms. the apparent molecu ... | 2005 | 15784981 |
| microbacterium natoriense sp. nov., a novel d-aminoacylase-producing bacterium isolated from soil in natori, japan. | a rod-shaped, gram-positive bacterium, strain tnjl143-2(t), having n-acyl-d-amino acid amidohydrolase (d-aminoacylase) activity, was isolated from a soil sample from natori, japan. it was a non-spore-forming, strictly aerobic bacterium without motility, showing a temperature optimum for growth of 30 degrees c and a ph optimum for growth of 5-7. the 16s rrna gene sequence of the strain showed the highest similarities to members of the genus microbacterium, in particular, microbacterium aerolatum, ... | 2005 | 15774640 |
| microbacterium oleivorans sp. nov. and microbacterium hydrocarbonoxydans sp. nov., novel crude-oil-degrading gram-positive bacteria. | a taxonomic study of two crude-oil-degrading, gram-positive bacterial strains, designated bas69(t) and bnp48(t), revealed that they represent two novel microbacterium species. 16s rrna gene sequence similarity to their closest phylogenetic neighbours was 98.5 % for bas69(t) (microbacterium paraoxydans dsm 15019(t) and microbacterium saperdae dsm 20169(t)) and 99 % for bnp48(t) (microbacterium luteolum dsm 20143(t)). levels of dna-dna relatedness to the closest phylogenetic neighbours of both str ... | 2005 | 15774639 |
| nitrile bioconversion by microbacterium imperiale cbs 498-74 resting cells in batch and ultrafiltration membrane bioreactors. | the biohydration of acrylonitrile, propionitrile and benzonitrile catalysed by the nhase activity contained in resting cells of microbacterium imperiale cbs 498-74 was operated at 5, 10 and 20 degrees c in laboratory-scale batch and membrane bioreactors. the bioreactions were conducted in buffered medium (50 mm na(2)hpo(4)/nah(2)po(4), ph 7.0) in the presence of distilled water or tap-water, to simulate a possible end-pipe biotreatment process. the integral bioreactor performances were studied w ... | 2006 | 15739103 |
| expression and purification of a recombinant enantioselective amidase. | microbacterium sp. aj115 metabolises a wide range of nitriles using the two-step nitrile hydratase/amidase pathway. in this study, the amidase gene of microbacterium sp. aj115 has been inserted into the pcal-n-ek expression vector and expressed in escherichia coli bl21(de3)plyss. the expressed protein is active in e. coli and expression of the amidase gene allows e. coli to grow on acetamide as sole carbon and/or nitrogen source. expression of active amidase in e. coli was temperature dependent ... | 2005 | 15721788 |
| microbacterium halotolerans sp. nov., isolated from a saline soil in the west of china. | a gram-positive short rod isolated from a saline soil in china was characterized using a polyphasic approach. this actinobacterium grew over a wide salinity range [0-15 % nacl, 0-20 % kcl and 0-30 % (mgcl2).6h2o (w/v); optimum concentrations for growth were 5 % nacl, 5-10 % kcl, 10 % (mgcl2).6h2o]. the optimum growth temperature and ph were 28-30 degrees c and 7.0-8.0, respectively. chemotaxonomic features (peptidoglycan-type b2beta with glycolyl residues; major menaquinones mk-10 and mk-11; pre ... | 2005 | 15653854 |
| effects of ph amendment on metal working fluid wastewater biological treatment using a defined bacterial consortium. | the aim of this study was to determine whether ph amendment of a highly alkaline metal working fluid (mwf) wastewater would improve biological treatment in a bioreactor system following introduction of a bacterial inoculum (comprised of the following strains: agrobacterium radiobacter, comamonas testosteroni, methylobacterium mesophilicum, microbacterium esteraromaticum, and microbacterium saperdae). the ph values tested were 6, 7, 8, and 9. three replicate batch mode bioreactors inoculated with ... | 2005 | 15625673 |
| production of dihydroxy c50-carotenoid by aureobacterium sp. ferm p-18698. | the dihydroxy c(50)-carotenoid, decaprenoxanthin, was produced by aureobacterium sp. collected from sea water. the addition of d-psicose to the culture medium improved the growth of cells and the yield of the carotenoid. the (13)c-nmr spectrum of decaprenoxanthin, which has not previously been reported, was successfully measured. | 2004 | 15618644 |
| bacterial diversity in spent mushroom compost assessed by amplified rdna restriction analysis and sequencing of cultivated isolates. | spent mushroom compost (smc) is the residual by-product of commercial agaricus spp. cultivation, and it is mainly composed of a thermally treated cereal straw/animal manure mixture colonized by the fungal biomass. research on the valorization of this material is mainly focusing on its use as soil conditioner and plant fertilizer. an investigation of the bacterial diversity in smc was performed using molecular techniques in order to reveal the origin of smc microflora and its potential effect on ... | 2004 | 15612633 |
| [microbacterium oxydans, a symbiont of djungarian hamster, displaying probiotic properties]. | a resident microorganism (strain kho-17) was isolated from secretions of the specific glandular structures at the angles of mouth of djungarian hamster (phodopus campbelli). according to cultural, morphological, and physiological properties as well as to the phylogenetic analysis basing on the sequences of 16s rrna gene and analysis of the cell wall the strain was assigned to the species microbacterium oxydans. the bacterium isolated displayed probiotic properties when administered orally as a s ... | 2004 | 15609853 |
| synthesis, solution behavior, thermal stability, and biological activity of an fe(iii) complex of an artificial siderophore with intramolecular hydrogen bonding networks. | previously, an artificial siderophore complex, the iron(iii) complex with tris[2-[(n-acetyl-n-hydroxy)glycylamino]ethyl]amine (tage), was constructed in order to understand the effect of intramolecular hydrogen bonding interaction on the siderophore function, and its structural characterization in the solid state was reported (inorg. chem. 2001, 40, 190). in this paper, the solution behavior of the m(iii)-tage (m = fe, ga) system has been investigated using (1)h nmr, uv-vis, and fab mass spectro ... | 2004 | 15606204 |
| bacterial diversity in a nonsaline alkaline environment: heterotrophic aerobic populations. | heterotrophic populations were isolated and characterized from an alkaline groundwater environment generated by active serpentinization, which results in a ca(oh)2-enriched, extremely diluted groundwater with ph 11.4. one hundred eighty-five strains were isolated in different media at different ph values during two sampling periods. to assess the degree of diversity present in the environment and to select representative strains for further characterization of the populations, we screened the is ... | 2004 | 15574939 |
| bacterial diversity of a soil sample from schirmacher oasis, antarctica. | the bacterial diversity of a soil sample collected in the vicinity of lake zub, schirmacher oasis, antarctica, was determined both by establishing pure colonies of culturable bacteria and by cloning the total 16s rdna of the soil and establishing the phylogeny of the clones. analysis of the 16s rrna gene clones indicated that the bacteria belonged to the classes alpha-proteobacteria, beta-proteobacteria, gamma-proteobacteria, gemmatimonas, bacteriodetes, actinobacteria, chloroflexi and chlamydia ... | 2004 | 15559969 |
| crystallization and preliminary x-ray analysis of pyridoxal 4-dehydrogenase, the second enzyme in degradation pathway i of pyridoxine. | pyridoxal 4-dehydrogenase (pldh; ec 1.1.107) is the second enzyme in the bacterial degradation pathway i of vitamin b(6), which catalyzes the oxidation of pyridoxal to 4-pyridoxolactone using nad(+). pldh from microbacterium luteolum, a dimeric protein with a subunit molecular weight of 38 kda, was crystallized at 277 k in a drop solution comprising 15%(w/v) polyethylene glycol 4000, 0.15 m sodium acetate, 7.5 mm n-octyl-beta-d-glucoside and 0.075 m tris-hcl ph 7.5 by the sitting-drop vapour-dif ... | 2004 | 15502327 |
| two novel species of tetrodotoxin-producing bacteria isolated from toxic marine puffer fishes. | out of eight dominant discrete bacterial colonies isolated and purified from the toxic marine puffer fishes collected in hong kong waters, two novel species of non-sporing, non-acid-fast and chemoorganotrophic bacteria capable of producing tetrodotoxin (ttx, a potent non-protein neurotoxin), as well as one previously reported and confirmed ttx-producing bacterium. they were identified as microbacterium arabinogalactanolyticum, serratia marcescens and vibrio alginolyticus, respectively, all of wh ... | 2004 | 15501290 |
| srf-3, a mutant of caenorhabditis elegans, resistant to bacterial infection and to biofilm binding, is deficient in glycoconjugates. | srf-3 is a mutant of c. elegans that is resistant to infection by microbacterium nematophilum and to binding of the biofilm produced by yersinia pseudotuberculosis and yersinia pestis. recently, srf-3 was characterized as a nucleotide sugar transporter of the golgi apparatus occurring exclusively in hypodermal seam cells, pharyngeal cells, and spermatheca. based on the above observations, we hypothesized that srf-3 may have altered glyconjugates that may enable the mutant nematode to grow unaffe ... | 2004 | 15452127 |
| the erk map kinase cascade mediates tail swelling and a protective response to rectal infection in c. elegans. | the nematode caenorhabditis elegans is proving to be an attractive model organism for investigating innate immune responses to infection. among the known pathogens of c. elegans is the bacterium microbacterium nematophilum, which adheres to the nematode rectum and postanal cuticle, inducing swelling of the underlying hypodermal tissue and causing mild constipation. we find that on infection by m. nematophilum, an extracellular signal-regulated kinase (erk) mitogen-activated protein (map) kinase ... | 2004 | 15268855 |
| characterization of a protease of a feather-degrading microbacterium species. | to characterize a new feather-degrading bacterium. | 2004 | 15242459 |
| molecular cloning, expression, and properties of an unusual aldo-keto reductase family enzyme, pyridoxal 4-dehydrogenase, that catalyzes irreversible oxidation of pyridoxal. | microbacterium luteolum yk-1 has pyridoxine degradation pathway i. we have cloned the structural gene for the second step enzyme, pyridoxal 4-dehydrogenase. the gene consists of 1,026-bp nucleotides and encodes 342 amino acids. the enzyme was overexpressed under cold shock conditions with a coexpression system and chaperonin groel/es. the recombinant enzyme showed the same properties as the m. luteolum enzyme. the primary sequence of the enzyme was 54% identical with that of d-threo-aldose 1-deh ... | 2004 | 15226311 |
| effect of levan's branching structure on antitumor activity. | levan produced from microbacterium laevaniformans kctc 9732 (m-levan) was isolated and treated with an inulinase to modify its branching structure. the chemical structures of levans were characterized, and the modified levans were applied on animal tumor cells to evaluate their antitumor activity. the gc-ms analysis indicated that beta-(2,1)-linked branches of m-levan were specifically hydrolyzed. as the ratio of applied inulinase to levan increased, the branching degree decreased proportionally ... | 2004 | 15225991 |
| studies on cultured and uncultured microbiota of wild culex quinquefasciatus mosquito midgut based on 16s ribosomal rna gene analysis. | to describe the midgut microbial diversity and the candidate bacteria for the genetic manipulation for the generation of transgenic mosquitoes refractory to transmission of diseases, the microbiota of wild culex quinquefasciatus mosquito midgut was studied using a conventional culture technique and analysis of a 16s ribosomal rna (rrna) gene sequence library. the culturable microbiota was identified as acinetobacter junii, ac. calcoaceticus, aeromonas culicicola, bacillus thuringiensis, microbac ... | 2004 | 15210998 |
| characterization of bacterial diversity in pulque, a traditional mexican alcoholic fermented beverage, as determined by 16s rdna analysis. | the bacterial diversity in pulque, a traditional mexican alcoholic fermented beverage, was studied in 16s rdna clone libraries from three pulque samples. sequenced clones identified as lactobacillus acidophilus, lactobacillus strain asf360, l. kefir, l. acetotolerans, l. hilgardii, l. plantarum, leuconostoc pseudomesenteroides, microbacterium arborescens, flavobacterium johnsoniae, acetobacter pomorium, gluconobacter oxydans, and hafnia alvei, were detected for the first time in pulque. identity ... | 2004 | 15183874 |
| antitumor activity of levan polysaccharides from selected microorganisms. | levans were isolated from the cultures of gluconoacetobacter xylinus (g-levan; mw = 40,000), microbacterium laevaniformans (m; mw = 710,000), rahnella aquatilis (r; mw = 380,000), and zymomonas mobilis (z; mw = 570,000). the levans were composed mainly of fructose residues when analyzed by tlc and hplc, and their main backbones were beta-(2,6)-linkages with beta-(2,1)-branches by gc-ms and nmr. in the in vitro antitumor activity test of the levans against eight different tumor cell lines, relati ... | 2004 | 15178007 |
| potentially pathogenic features of heterotrophic plate count bacteria isolated from treated and untreated drinking water. | heterotrophic plate counts (hpcs) are commonly used to assess the general microbiological quality of drinking water. drinking water quality specifications worldwide recommend hpc limits from 100 to 500 cfu ml(-1). a number of recent studies revealed evidence that these bacteria may not be as harmless as generally accepted. it appears that immuno-compromised individuals are particularly at risk. this would include the very young and very old patients with diseases such as aids and patients on the ... | 2004 | 15145586 |
| isolation and characterization of novel bacteria degrading polycyclic aromatic hydrocarbons from polluted greek soils. | three bacterial strains, designated as wphe1, sphe1, and ophe1, were isolated from greek soils contaminated with polycyclic aromatic hydrocarbon (pah)-containing waste from the wood processing, steel, and oil refinery industries. wphe1, sphe1, and ophe1 were characterized and identified as species of pseudomonas, microbacterium, and paracoccus, respectively, based on gram staining, biochemical tests, phospholipid analysis, fame analysis, g+c content and 16s rrna gene sequence analysis. the resul ... | 2004 | 15133642 |
| loss of srf-3-encoded nucleotide sugar transporter activity in caenorhabditis elegans alters surface antigenicity and prevents bacterial adherence. | during the establishment of a bacterial infection, the surface molecules of the host organism are of particular importance, since they mediate the first contact with the pathogen. in caenorhabditis elegans, mutations in the srf-3 locus confer resistance to infection by microbacterium nematophilum, and they also prevent biofilm formation by yersinia pseudotuberculosis, a close relative of the bubonic plague agent yersinia pestis. we cloned srf-3 and found that it encodes a multitransmembrane hydr ... | 2004 | 15123614 |
| improved production of bioactive glucosylmannosyl-glycerolipid by sponge-associated microbacterium species. | the marine microbacterium species hp2 (dsm 12583), isolated from the sponge halichondria panicea, is able to produce a glucosylmannosyl-glycerolipid when grown on a complex medium with glucose. optimizing the carbon sources in shake flask experiments has shown that glycerol affords the highest specific glycoglycerolipid production. the product yield approached 300 mg/l or 25 mg/g biomass upon scaling up in a 40-l bioreactor volume. the native diglycosyl-glycerolipid ggl.2 strongly inhibited grow ... | 2004 | 15085410 |
| the crystal structure of a (-) gamma-lactamase from an aureobacterium species reveals a tetrahedral intermediate in the active site. | the structure of the recombinant (-) gamma-lactamase from an aureobacterium species has been solved at 1.73a resolution in the cubic space group f23 with unit cell parameters a=b=c=240.6a. the trimeric enzyme has an alpha/beta hydrolase fold and closely resembles the cofactor free haloperoxidases. the structure has been solved in complex with a covalently bound ligand originating from the host cell and also in the unligated form. the associated density in the former structure has been interprete ... | 2004 | 15081810 |
| microbacterium ulmi sp. nov., a xylanolytic, phosphate-solubilizing bacterium isolated from sawdust of ulmus nigra. | a xylanolytic and phosphate-solubilizing bacterium isolated from sawdust of ulmus nigra in salamanca was characterized by a polyphasic approach. the novel strain, designated xil02(t), was gram-positive, aerobic, catalase- and oxidase-negative, rod-shaped and non-motile. phylogenetically and chemotaxonomically, it was related to members of the genus microbacterium. according to 16s rrna gene sequence analysis, it is closely related to microbacterium arborescens and microbacterium imperiale; howev ... | 2004 | 15023968 |
| coenzyme precursor-assisted cooperative overexpression of an active pyridoxine 4-oxidase from microbacterium luteolum. | the overexpression system of the active pyridoxine 4-oxidase from microbacterium luteolum was developed. when chaperonin groel/es genes in plasmid pky206 were coexpressed, the pyridoxine 4-oxidase gene cloned in the vector ptrc99a was overexpressed in escherichia coli jm109 cultured in lb medium containing 50microm riboflavin, the precursor of coenzyme (fad) of the enzyme, under the cold stress at 23 degrees c. the crude extract from the cotransformant cells showed 88-fold higher specific activi ... | 2004 | 15003257 |