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expression of fusion proteins of aspergillus terreus reveals a novel allene oxide synthase.aspergilli oxidize c18 unsaturated fatty acids by dioxygenase-cytochrome p450 fusion proteins to signal molecules involved in reproduction and host-pathogen interactions. aspergillus terreus expresses linoleate 9r-dioxygenase (9r-dox) and allene oxide synthase (aos) activities in membrane fractions. the genome contains five genes (ateg), which may code for a 9r-dox-aos fusion protein. the genes were cloned and expressed, but none of them oxidized 18:2n-6 to 9r-hydroperoxy-10(e),12(z)-octadecadie ...201323479731
aspergillus terreus complex: an emergent opportunistic agent of onychomycosis.the incidence of onychomycosis due to non-dermatophyte moulds (ndm) is increasing. aspergillus terreus is relatively undocumented as an agent of this fungal infection. the aim of this work is to show the prevalence of onychomycosis caused by a. terreus and to describe its clinical features. nail samples were collected for microscopic examination and culturing in selective media. all cases of onychomycosis due to ndm were confirmed by a second sample. aspergillus terreus isolates were identified ...201323448599
terrein induces apoptosis in hela human cervical carcinoma cells through p53 and erk regulation.terrein, a fungal metabolite derived from aspergillus terreus, has been shown to have a variety of biological activities in human cells including inhibition of melanogenesis, as well as anti-inflammatory, antioxidant and anticancer properties. in the present study, terrein was shown to have marked anticancer activity on hela human cervical carcinoma cells. terrein exhibited inhibition of proliferation within the same ranges for other cancer cell types with an ic50 at 0.29 mm. the growth inhibiti ...201323417151
aspulvinones from a mangrove rhizosphere soil-derived fungus aspergillus terreus gwq-48 with anti-influenza a viral (h1n1) activity.a new butenolide isoaspulvinone e (1), together with two known butenolides aspulvinone e (2) and pulvic acid (3) were isolated from the marine-derived fungus, aspergillus terreus gwq-48. they showed significant anti-influenza a h1n1 virus activities, with ic50 values of 32.3, 56.9, and 29.1μg/ml, respectively. moreover, only compound 1 exhibited effective inhibitory activity against h1n1 viral neuraminidase (na), and docking of two isomers (1-2) into the active sites of na showed that the e doub ...201323411074
high-dose induction liposomal amphotericin b followed by de-escalation is effective in experimental aspergillus terreus pneumonia.aspergillus terreus is considered to be resistant to amphotericin b (amb). however, it is unknown whether higher daily doses of liposomal amb (l-amb) can overcome this resistance in vivo. we evaluated the efficacy and total lung homogenate amb concentrations of escalating intravenous doses of l-amb (3-20 mg/kg daily) versus an induction-de-escalation dosing strategy (10 mg/kg/day ×3 days, then 3 mg/kg/day) in an experimental neutropenic murine model of a. terreus pneumonia.201323349440
severe bacterial superinfection based on influenza a (h1n1) pneumonia in a heart-lung transplant recipient.a 47-year-old heart-lung transplant recipient presented to our outpatient transplant clinic with respiratory infection. her nose and throat swabs for influenza a (h1n1) infection were negative. broncheoalveolar lavage showed a positive result for h1n1 infection. antiviral therapy was initiated. because of superinfection with pseudomonas aeruginosa and aspergillus terreus, her clinical condition worsened. the clinical condition of the patient improved with antibiotic and antifungal treatment. neg ...201323344758
characterization of the biosynthetic genes for 10,11-dehydrocurvularin, a heat shock response-modulating anticancer fungal polyketide from aspergillus terreus.10,11-dehydrocurvularin is a prevalent fungal phytotoxin with heat shock response and immune-modulatory activities. it features a dihydroxyphenylacetic acid lactone polyketide framework with structural similarities to resorcylic acid lactones like radicicol or zearalenone. a genomic locus was identified from the dehydrocurvularin producer strain aspergillus terreus ah-02-30-f7 to reveal genes encoding a pair of iterative polyketide synthases (a. terreus curs1 [atcurs1] and atcurs2) that are pred ...201323335766
new insight into amphotericin b resistance in aspergillus terreus.amphotericin b (amb) is the predominant antifungal drug, but the mechanism of resistance is not well understood. we compared the in vivo virulence of an amb-resistant aspergillus terreus (atr) isolate with that of an amb-susceptible a. terreus isolate (ats) using a murine model for disseminated aspergillosis. furthermore, we analyzed the molecular basis of intrinsic amb resistance in vitro by comparing the ergosterol content, cell-associated amb levels, amb-induced intracellular efflux, and proo ...201323318794
itaconic acid--a biotechnological process in change.in the last years, itaconic acid has gained increasing interest as future bio-based platform chemical. to replace petrol-based compounds such as methacrylic acid in industry, the economic efficiency of the current biotechnological production processes with the fungus aspergillus terreus has to be improved. the recent progress in understanding the biosynthesis, the regulation and the cellular transport of itaconic acid has facilitated the optimisation of existing processes as well as the construc ...201323298766
mic values of voriconazole are predictive of treatment results in murine infections by aspergillus terreus species complex.we evaluated the efficacy of voriconazole against nine strains of aspergillus terreus with different mics (0.12 to 4 μg/ml) by using a murine model. markers of efficacy included survival, tissue burden, galactomannan antigenemia, and drug serum levels. voriconazole was especially effective in prolonging survival and reducing the fungal load in infections by strains that showed mics that were less than or equal to the epidemiological cutoff value (1 μg/ml). in vitro data might be useful for predi ...201323295929
structure revision of asperjinone using computer-assisted structure elucidation methods.the elucidated structure of asperjinone (1), a natural product isolated from thermophilic aspergillus terreus, was revised using the expert system structure elucidator. the reliability of the revised structure (2) was confirmed using 180 structures containing the (3,3-dimethyloxiran-2-yl)methyl fragment (3) as a basis for comparison and whose chemical shifts contradict the suggested structure (1).201323289877
coupled production of single cell oil as biodiesel feedstock, xylitol and xylanase from sugarcane bagasse in a biorefinery concept using fungi from the tropical mangrove wetlands.this work evaluates sugarcane bagasse (scb) conversion, in a biorefinery approach, to coproduce biodiesel and high value products using two novel mangrove fungi. on acid pre-treatment, sugarcane bagasse hydrolysate (scbh) resulted in a xylitol yield of 0.51 g/g xylose consumed in 72 h by williopsis saturnus. after scb pretreatment, sugarcane bagasse residue (scbr) was utilized using aspergillus terreus for production of xylanase (12.74 u/ml) and cell biomass (9.8 g/l) which was extracted for sin ...201323260270
isolation and characterization of three fungi with the potential of transforming glycyrrhizin.three fungi with different types of transformation of glycyrrhizin (gl) were isolated from the soil samples of glycyrrhiza glabra planting area in china. according to their morphologies and 18 s rdna gene sequence analysis, the three fungi were identified and named as penicillium purpurogenum li-3, aspergillus terreus li-20 and aspergillus ustus li-62. transforming products analysis by tlc and hplc-ms indicated that p. purpurogenum li-3, a. terreus li-20 and a. ustus li-62 could stably transform ...201323247917
a novel pathway construction in candida tropicalis for direct xylitol conversion from corncob xylan.in this study, an integrated xylitol production pathway, directly using xylan as the substrate, was constructed in candida tropicalis bit-xol-1 which could efficiently convert xylose into xylitol. in order to consolidate this bioprocessing, a β-1,4-xylanase gene (atn) and a β-xylosidase gene (atl) were cloned from aspergillus terreus, and were constructed onto episomal plasmid paur123. additionally, combination of the individual atn and atl expression cassette was also cloned onto paur123. after ...201323211479
butyrolactone and cycloheptanetrione from mangrove-associated fungus aspergillus terreus.a new butyrolactone, 7″-hydroxybutyrolactone iii (1) and three new cycloheptanetriones, terretrione a-c (2-4), together with five known compounds, butyrolactone i, cyclo(leu-pro), cyclo(val-pro), cyclo(ile-pro), cyclo(phe-pro), were isolated from mangrove-associated marine fungus aspergillus terreus. the structures of these compounds were elucidated on the basis of physical data analysis (nmr, high resolution-electrospray ionization (hr-esi)-ms), especially by 2d-nmr techniques. these compounds ...201223124567
lovastatin production by aspergillus terreus using agro-biomass as substrate in solid state fermentation.ability of two strains of aspergillus terreus (atcc 74135 and atcc 20542) for production of lovastatin in solid state fermentation (ssf) using rice straw (rs) and oil palm frond (opf) was investigated. results showed that rs is a better substrate for production of lovastatin in ssf. maximum production of lovastatin has been obtained using a. terreus atcc 74135 and rs as substrate without additional nitrogen source (157.07 mg/kg dry matter (dm)). although additional nitrogen source has no benefit ...201223118499
enhanced production of (+)-terrein in fed-batch cultivation of aspergillus terreus strain pf26 with sodium citrate.(+)-terrein is a fungal metabolite with multiple biological activities, especially with great value in medicine. however, the mass production of single configuration terrein is still a big challenge. in this study, the effects of acetic acid, sodium acetate, citric acid and sodium citrate on the (+)-terrein production by aspergillus terreus strain pf26 derived from marine sponge phakellia fusca were investigated. sodium citrate was selected for fed-batch cultivation because it showed the best ef ...201323085955
sexual reproduction in the opportunistic human pathogen aspergillus terreus.aspergillus terreus has a worldwide distribution in soil, constitutes the third most important cause of invasive aspergillosis in humans and is classically regarded as a strictly asexual species. strains of a. terreus were characterized by its, β-tubulin and calmodulin sequences. mating type was identified by amplifying and sequencing mat1-1 and mat1-2 genes. one mat1-2 strain produced hyphal masses when crossed with four mat1-1 strains on mixed cereal agar after 3 wk at 37 c. cleistothecia form ...201323074177
evolutionary imprint of catalytic domains in fungal pks-nrps hybrids.fungal hybrid enzymes consisting of a polyketide synthase (pks) and a nonribosomal peptide synthetase (nrps) module are involved in the biosynthesis of a vast array of ecologically and medicinally relevant natural products. whereas a dozen gene clusters could be assigned to the requisite pks-nrps pathways, the programming of the multifunctional enzymes is still enigmatic. through engineering and heterologously expressing a chimera of pks (lovastatin synthase, lovb) and nrps (cytochalasin synthas ...201223023987
effect of ph on biosynthesis of lovastatin and other secondary metabolites by aspergillus terreus atcc 20542.the effect of the initial ph value of the cultivation medium on lovastatin (mevinolinic acid) biosynthesis by aspergillus terreus atcc20542 was studied. it was found that if the ph value of the broth is acidic, the direct chromatographic assay of mevinolinic acid leads to the underestimated values. thus, the equilibrium curve was determined for the transformation of β-hydroxy acid form of lovastatin (mevinolinic acid) into lovastatin lactone. the calculation of the equilibrium constant shows tha ...201222995742
furandiones from an endophytic aspergillus terreus residing in malus halliana.two new furandiones named asperterone b (1) and c (2) together with four known metabolites (3-6) were isolated from the liquid culture of the endophytic fungus aspergillus terreus mhl-p22 residing in the fresh leaves of malus halliana. the structures of the new compounds were elucidated by analysis of their ms, ir, 1d- and 2d-nmr spectra. 1 and 2 showed moderate cytotoxic activities against human colorectal carcinoma sw1116 cells with ic(50) values of 57.5 and 71.0 µm, respectively. the biosynth ...201222971827
animal bone char solubilization with itaconic acid produced by free and immobilized aspergillus terreus grown on glycerol-based medium.cells of aspergillus terreus, free and immobilized in polyurethane foam, were employed in itaconic acid fermentation processes on glycerol-based media. the purpose was to assess their suitability for animal bone char solubilization and the development of a biotechnological alternative to p fertilizers chemically produced from rock phosphate. animal bones constitute a renewable source of p that can replace the traditionally used finite, nonrenewable rock phosphate as a p source. glycerol was an e ...201222956279
a new β-glucuronidase inhibiting butyrolactone from the marine endophytic fungus aspergillus terreus.an endophytic fungus aspergillus terreus, var. boedijnii (blochwitz) was isolated from red marine alga laurencia ceylanica, j. agardh, cultured in large scale and extracted with etoac. the above-mentioned extract yielded a new butyrolactone, 3-hydroxy-4-(4-hydroxyphenyl)-5-methoxycarbonyl-5-(4-hydroxy-3-formylbenzyl)-2,5-dihydro-2-furanone (1), along with the previously reported nine compounds, butyrolactone-1 (2), 6-hydroxymellin (3), (3 r, 4 r)-6,7-dimethoxy-4-hydroxymellin (4), (+)-territonin ...201322913423
production of cellulases by solid state fermentation with aspergillus terreus and enzymatic hydrolysis of mild alkali-treated rice straw.rice straw was used as substrate for cellulase production by solid state fermentation with aspergillus terreus. substrate concentration, moisture ratio, inoculum size and initial ph were optimized using response surface methodology involving box-behnken design. the predicted filter paper activity under optimized parameters was 9.73 u/g and the validated filter paper activity was 10.96 u/g. hydrolysis of the biomass pretreated with 0.125% to 1% naoh for 24h at room temperature was performed using ...201222864171
studies on the metabolism of five model drugs by fungi colonizing cadavers using lc-esi-ms/ms and gc-ms analysis.it is well-known that cadavers may be colonized by microorganisms, but there is limited information if or to what extent these microbes are capable of metabolizing drugs or poisons, changing the concentrations and metabolic pattern of such compounds in postmortem samples. the aim of the present study was to develop a fungal biotransformation system as an in vitro model to investigate potential postmortem metabolism by fungi. five model drugs (amitriptyline, metoprolol, mirtazapine, promethazine, ...201222825674
antimicrobial and synergistic effects of silver nanoparticles synthesized using soil fungi of high altitudes of eastern himalaya.fifty three fungi isolated from soils of different microhabitats of eastern himalayan range (3,400~3,600 msl) were screened for mycosynthesis of silver nanaoparticles (agnps) and their efficacy as antimicrobials were assessed in combination with commonly used antibiotics. three isolates aspergillus terreus sp5, paecilomyces lilacinus sf1 and fusarium sp. mp5 identified based on morphological and 18s rrna gene sequences were found to synthesize agnps. these nanoparticles were characterized by vis ...201222783131
in vivo emergence of aspergillus terreus with reduced azole susceptibility and a cyp51a m217i alteration.azole resistance in aspergillus terreus isolates was explored. twenty related (mb) and 6 unrelated a. terreus isolates were included. cyp51a sequencing and rapd genotyping was performed. five mb isolates were itraconazole susceptible, whereas the minimum inhibitory concentrations (mics) for 15 mb isolates were elevated (1-2 mg/l). voriconazole and posaconazole mics were 0.5-4 and 0.06-0.5 mg/l, respectively, for mb isolates but 0.25-0.5 and <0.03-0.06 mg/l, respectively, for controls. sequencing ...201222782947
microbial transformation of the sesquiterpene lactone tagitinin c by the fungus aspergillus terreus.the biotransformation of the sesquiterpene lactone tagitinin c by the fungus aspergillus terreus mt 5.3 yielded a rare derivative that was elucidated by spectrometric methods. the fungus led to the formation of a different product through an unusual epoxidation reaction between c4 and c5, formation of a c3,c10 ether bridge, and a methoxylation of the c1 of tagitinin c. the chemical structure of the product, namely 1β-methoxy-3α-hydroxy-3,10β-4,5α-diepoxy-8β-isobutyroyloxygermacr-11(13)-en-6α,12- ...201222782617
a small molecule ifb07188 inhibits proliferation of human cancer cells by inducing g2/m cell cycle arrest and apoptosis.a small molecule, 15b-β-hydroxy-5-n-acetylardeemin (ifb07188), was previously isolated from the fungi aspergillus terreus. however, the toxicological features of this natural product have never been investigated. in present study, we described the anticancer activities of ifb07188. we found that ifb07188 can decrease the viability and invasive potency of multiple cancer cell lines. further mechanistic investigation demonstrates that cell cycles are arrested at g2/m phase and the arrest is induce ...201222770989
microbial production of itaconic acid: developing a stable platform for high product concentrations.biotechnologically produced itaconic acid (ia) is a promising organic acid with a wide range of applications and the potential to open up new application fields in the area of polymer chemistry, pharmacy, and agriculture. in this study, a systematic process optimization was performed with an own isolated strain of aspergillus terreus and transferred from a 250-ml to a 15-l scale. an ia concentration of 86.2 g/l was achieved within 7 days with an overall productivity of 0.51 g/(l h), a maximum pr ...201222752264
biosynthesis of xylobiose: a strategic way to enrich the value of oil palm empty fruit bunch fiber.xylooligosaccharides are functional foods mainly produced during the hydrolysis of xylan by physical, chemical, or enzymatic methods. in this study, production of xylobiose was investigated using oil palm empty fruit bunch fiber (opefb) as a source material, by chemical and enzymatic methods. xylanase-specific xylan hydrolysis followed by xylobiose production was observed. among different xylanases, xylanase from fxy-1 released maximum xylobiose from pretreated opefb fiber, and this fungal strai ...201222713984
single cell oil of oleaginous fungi from the tropical mangrove wetlands as a potential feedstock for biodiesel.single cell oils (scos) accumulated by oleaginous fungi have emerged as a potential alternative feedstock for biodiesel production. though fungi from mangrove ecosystem have been reported for production of several lignocellulolytic enzymes, they remain unexplored for their sco producing ability. thus, these oleaginous fungi from the mangrove ecosystem could be suitable candidates for production of scos from lignocellulosic biomass. the accumulation of lipids being species specific, strain select ...201222646719
optimization of culture conditions and bench-scale production of l-asparaginase by submerged fermentation of aspergillus terreus mtcc 1782.optimization of culture conditions for l-asparaginase production by submerged fermentation of aspergillus terreus mtcc 1782 was studied using a 3-level central composite design of response surface methodology and artificial neural network linked genetic algorithm. the artificial neural network linked genetic algorithm was found to be more efficient than response surface methodology. the experimental l-asparaginase activity of 43.29 iu/ml was obtained at the optimum culture conditions of temperat ...201222580311
operating conditions optimization for (+)-terrein production in a stirred bioreactor by aspergillus terreus strain pf-26 from marine sponge phakellia fusca.terrein is a fungal metabolite with application values in the fields of medicine, cosmetology, and agriculture. however, mass production of single configuration terrein is still a big challenge. in this study, operating factors such as inoculation, agitation speed, aeration rate, ph control, and nutrient feeding were preliminarily optimized to improve the (+)-terrein production in the 5-l stirred bioreactor from the marine sponge-derived fungus aspergillus terreus pf-26. spore inoculation, low a ...201222527032
rapid identification of aspergillus terreus from bronchoalveolar lavage fluid by pcr and electrospray ionization with mass spectrometry.we describe the application of pcr and electrospray-ionization with mass spectrometry (pcr/esi-ms) to culture-negative bronchoalveolar lavage (bal) fluid in order to identify septate hyphae noted by gomori methenamine silver (gms) staining of the fluid that was obtained from an immunocompromised woman with neutropenia following induction chemotherapy for treatment of acute myelogenous leukemia (aml). the patient was treated with empirical antifungal therapy, including intrathecal amphotericin b, ...201222518857
screening of medicinal higher basidiomycetes mushrooms from turkey for lovastatin production.as a first attempt, a study was carried out to test for lovastatin production ability in local higher basidiomycetes mushroom isolates from turkey. an extended screening was performed for lovastatin production in yeast lactose agar medium, among a total of 136 macrofungi isolates from the basidiomycetes culture collection of eskisehir osmangazi university. lovastatin production was evaluated by disc diffusion method and was also confirmed by tlc and hplc. only six isolates were found to be lovas ...201222506575
murine infection models for aspergillus terreus pulmonary aspergillosis reveal long-term persistence of conidia and liver degeneration.aspergillus terreus is emerging as a causative agent of life-threatening invasive aspergillosis. prognosis for affected patients is often worse than for a. fumigatus infections. to study a. terreus-mediated disease, we developed 3 infection models. in embryonated hen's eggs and leucopenic mice, the outcome of invasive aspergillosis was similar to that described for a. fumigatus. however, 10(2)- and 10(3)-fold higher conidia concentrations were required for 100% lethality. in corticosteroid-treat ...201222438397
aspergillus terreus: how inoculum size and host characteristics affect its virulence. 201222438395
characterization of hemicellulases from thermophilic fungi.the thermophilic fungi thermomyces lanuginosus, malbranchea cinnamomea, myceliophthora fergusii and the thermotolerant aspergillus terreus were cultivated on various carbon sources, and hemicellulolytic and cellulolytic enzyme profiles were evaluated. all fungi could grow on locust bean galactomannan (lbg), solka floc, wheat bran and pectin, except t. lanuginosus, which failed to utilize lbg for growth. different levels of cellulase and hemicellulase activities were produced by these fungal stra ...201222371150
asperjinone, a nor-neolignan, and terrein, a suppressor of abcg2-expressing breast cancer cells, from thermophilic aspergillus terreus.breast cancer cells express abcg2 transporters, which mediate multidrug resistance. discovering a novel compound that can suppress abcg2 expression and restore drug sensitivity could be the key to improving breast cancer therapeutics. in the current work, one new nor-neolignan, asperjinone (1), as well as 12 other known compounds, was isolated from aspergillus terreus. the structure of the new isolate was determined by spectroscopic methods. among these isolates, terrein (2) displayed strong cyt ...201222360613
biosorption of strontium from aqueous solution by fungus aspergillus terreus.the biosorption characteristics of strontium ions using fungus aspergillus terreus were investigated. experimental parameters affecting the biosorption process such as ph, contact time, initial metal concentration, and temperature were studied.201222322289
[studies on metabolites from marine microorganism aspergillus terreus collected from nature reserve region of mangrove].to search for new antitumor active lead compounds from marine microorganism.201122256757
disseminated aspergillosis in a dog due to aspergillus alabamensis.disseminated aspergillosis is uncommon in dogs and often associated with aspergillus terreus. a case of disseminated disease in an english springer spaniel is reported from which aspergillus alabamensis was recovered by culture and identified by molecular means suggesting a potential role for this agent as a primary pathogen of dogs.201224371723
physiological studies on carboxymethyl cellulase formation by aspergillus terreus dsm 826.physiological studies were conducted to determine the optimum cultural conditions for maximal carboxymethyl cellulase (cmcase) formation by aspergillus terreus dsm 826. shaking condition at 150 rpm is favorable for the production of cmcase from rice straw and sugar cane bagasse. the highest enzyme yield was obtained at the third day of incubation at 30 °c for both cases; however cmcase formation occurred at a broad range of ph values, with maximal formation of a. terreus dsm 826 cmcase at ph 4.5 ...201224031798
modification of c and n sources for enhanced production of cyclosporin 'a' by aspergillus terreus.most of the studies regarding cyclosporin 'a' production through fungi concentrate around tolypocladium inflatum. this is mainly due to lower reported production of this drug in other fungi. the present study was therefore conducted to explore indigenous isolates of aspergillus terreus for synthesis of this drug and defining a production medium for obtaining high yield of cyclosporin 'a'. for this purpose carbon and nitrogen sources were optimized for the selected best strain of a. terreus. over ...201124031766
optimization of lipase production from aspergillus terreus by response surface methodology and its potential for synthesis of partial glycerides under solvent free conditions.aspergillus terreus produces lipase 7.01 iu/ml in 96 h after optimization by one variable at a time method. using the significant factors i.e. corn oil (a), sodium nitrate (b), casein (c), agitation rate (d) and incubation period (e) rsm was carried out resulting in 19.65 iu/ml from the combination +1(a), -1(b), -1(c), +1(d) and 0(e). the interactions between sodium nitrate, casein and agitation with corn oil were most significant. scale up of production from 250 ml shake flask to 30 l bioreacto ...201022282615
response surface methodology for optimization of production of lovastatin by solid state fermentation.lovastatin, an inhibitor of hmg-coa reductase, was produced by solid state fermentation (ssf) using a strain of aspergillus terreus uv 1718. different solid substrates and various combinations thereof were evaluated for lovastatin production. wheat bran supported the maximum production (1458 ± 46 µg g(-1) dfm) of lovastatin. response surface methodology (rsm) was applied to optimize the medium constituents. a 2(4) full-factorial central composite design (ccd) was chosen to explain the combined e ...201024031477
bioconversion of straw into improved fodder: mycoprotein production and cellulolytic acivity of rice straw decomposing fungi.sixty two out of the sixty four species of fungal isolates tested could produce both exo-β1,4-gluconase (c1) and endo-β1,4-gluconase (cx) on pure cellulose and rice straw as carbon source in czapek's medium. fifty-eight and fifteen species were able to grow at 25℃ and at 45℃, respectively. eleven species could grow at both 25℃ and 45℃ while, four species appeared only at 45℃. the most cellulolytic species at 25℃ was trichoderma koningii producing 1.164 c1 (mg glucose/1 ml culture filtrate/1 hr) ...200524049480
aspergillus terreus and its toxic metabolites as a food contaminant in some egyptian bakery products and grains.a. terreus isolates isolated from some bakery products, corn and rice were found to be able to produce territrems. 90% of thea. terreus isolated from bakery products were able to produce territrem a, with a mean of 0.09 ppm, while 80% ofa. terreus isolates produce territrem b with a mean of 0.24 ppm. on the other hand 31.8% of the isolates ofa. terreus from corn were able to produce territrem a with a mean of 0.44 ppm. concerninga. terreus isolates from rice, 66.7% were found to produce territre ...199823605064
production of phenolic compounds by aspergillus s-4 in sake cake medium and identification of terrein.high performance liquid chromatography (hplc) analysis of the culture filtrate of an isolated mold, aspergillus terreus s-4, showed a larger peak of a terrein-like substance than those of gallic acid and protocatechuic acid. the chemical structure of this substance was investigated and identified as terrein, 4,5-dihydroxy-3-propenyl- 2-cyclopenten-1-one. we also examined some culture conditions for the production of terrein using a sake cake medium for strain s-4.199327281008
bioconversion of aflatoxin g1 to aflatoxin b 3 by selected fungi.aflatoxin g1 (afg1) was transformed into aflatoxin b3 (afb3) by the fungirhodotorula sp,sporobolomyces sp,rhizopus oryzae nrrl395,pythium ultimum, aspergillus terreus, a clavatus and penicillium frequentans grown in a medium containing afg1, difco potato dextrose broth, yeast extract, and peptone both in liquid shaken cultures and in solid static cultures at 25°c in the dark. a maximum rate of transformation of 10 % was obtained after 2 to 3 weeks of incubation. the transformation was correlated ...199023605445
chromatographic quantitation of territrems a, b, and c, tremorgenic mycotoxins from aspergillus terreus.this paper describes assay procedures for territrems a, b, and c by thin layer chromatography (tlc)-fluorodensitometry and reverse phase high performance liquid chromatography (hplc).198723604807
antibiotic-producing properties of aspergillus terreus. 195424543173
progress in definition, prevention and treatment of fungal infections in cystic fibrosis.cystic fibrosis (cf) is a chronic lethal multi-system condition; however, most of the morbidity and mortality is dependent on the status of the respiratory system. progressive respiratory decline is mediated by chronic infection and inflammation, punctuated by important acute events known as pulmonary exacerbations which can lead to accelerated decline. the main bacterial species causing infections include pseudomonas aeruginosa, staphylococcus aureus, haemophilus influenzae and achromobacter xy ...201728762125
evaluation of the in vitro activity of isavuconazole and comparator voriconazole against 2635 contemporary clinical candida and aspergillus isolates.the in vitro activity of isavuconazole was determined for 1677 candida and 958 aspergillus isolates from 2012 to 2014 with voriconazole as comparator.201728373148
notable fibrolytic enzyme production by aspergillus spp. isolates from the gastrointestinal tract of beef cattle fed in lignified pastures.fungi have the ability to degrade vegetal cell wall carbohydrates, and their presence in the digestive tract of ruminants can minimize the effects of lignified forage on ruminal fermentation. here, we evaluated enzyme production by aspergillus spp. isolates from the digestive tracts of cattle grazed in tropical pastures during the dry season. filamentous fungi were isolated from rumen and feces by culture in cellulose-based medium. ninety fungal strains were isolated and identified by rdna seque ...201728850605
a comparative study on the activity of fungal lytic polysaccharide monooxygenases for the depolymerization of cellulose in soybean spent flakes.lytic polysaccharide monooxygenases (lpmos) are copper-dependent enzymes capable of the oxidative breakdown of polysaccharides. they are of industrial interest due to their ability to enhance the enzymatic depolymerization of recalcitrant substrates by glycoside hydrolases. in this paper, twenty-four lytic polysaccharide monooxygenases (lpmos) expressed in trichoderma reesei were evaluated for their ability to oxidize the complex polysaccharides in soybean spent flakes, an abundant and industria ...201728750348
molecular basis for the unusual ring reconstruction in fungal meroterpenoid biogenesis.trt14 from aspergillus terreus is involved in unusual skeletal reconstruction during the biosynthesis of the fungal meroterpenoid terretonin. detailed in vitro characterization revealed that this novel multifunctional enzyme catalyzes not only the d-ring expansion via intramolecular methoxy rearrangement, but also the hydrolysis of the expanded d-ring. the x-ray crystal structures of trt14, in complex with substrate or product, and two trt14 homologs, aush and prhc from aspergillus nidulans and ...201728759016
proteomics as a tool to identify new targets against aspergillus and scedosporium in the context of cystic fibrosis.cystic fibrosis (cf) is a genetic disorder that increases the risk of suffering microbial, including fungal, infections. in this paper, proteomics-based information was collated relating to secreted and cell wall proteins with potential medical applications from the most common filamentous fungi in cf, i.e., aspergillus and scedosporium/lomentospora species. among the aspergillus fumigatus secreted allergens, β-1,3-endoglucanase, the alkaline protease 1 (alp1/oryzin), asp f 2, asp f 13/15, chiti ...201728484941
a case of bilateral otomycosis associated with aspergillus flavus and a. terreus in taiwan.otitis externa caused by fungi (otomycosis) occurs more commonly in tropical areas with high moisture than in temperate regions. bilateral otomycosis is, however, rarely reported. in a case of bilateral otitis externa in a 56-year-old male patient in taiwan, direct microscopic examination of the cerumen as well as isolation of strains indicated the presence of two aspergillus species being different in each of both ears. the species were identified by dna sequence comparisons and additional morp ...201728501466
[one-step fermentation for producing simvastatin via rnai silencing of lovf gene in aspergillus terreus].simvastatin is one of the important prescription drugs for hypercholesterolemia. monacolin j is a key intermediate during simvastatin synthesis, and also an intermediate of lovastatin biosynthesis. in this work, we construct a monacolin j producing strain via rna interference to achieve one-step fermentation to obtain simvastatin. the lovf gene silencing plasmid pmhj137 was constructed and transformed into aspergillus terreus by agrobacterium tumefaciens mediated transformation method. precursor ...201628853269
comparative study of the production of extracellular β-glucosidase by four different strains of aspergillus using submerged fermentation.four strains of aspergillus (aspergillus niger cdbb-h-176, a. niger cdbb-h-175, a. niger atcc 9642, and aspergillus terreus cdbb-h-194) were used to produce extracellular β-glucosidase. using an orthogonal experimental design (l9), we optimized the parameters of culture medium to maximize the activity of β-glucosidase. the optimal conditions (same for the four strains) were as follows: temperature, 30°c; ph, 6.0; orbital agitation, 200 rpm; concentration of sucrose, 0.5% (w/v). the most producti ...201728631979
repurposing antipsychotic drugs into antifungal agents: synergistic combinations of azoles and bromperidol derivatives in the treatment of various fungal infections.as the number of hospitalized and immunocompromised patients continues to rise, invasive fungal infections, such as invasive candidiasis and aspergillosis, threaten the life of millions of patients every year. the azole antifungals are currently the most prescribed drugs clinically that display broad-spectrum antifungal activity and excellent oral bioavailability. yet, the azole antifungals have their own limitations and are unable to meet the challenges associated with increasing fungal infecti ...201728797882
key role of alternative oxidase in lovastatin solid-state fermentation.lovastatin is a commercially important secondary metabolite produced by aspergillus terreus, either by solid-state fermentation or by submerged fermentation. in a previous work, we showed that reactive oxygen species (ros) accumulation in idiophase positively regulates lovastatin biosynthetic genes. in addition, it has been found that lovastatin-specific production decreases with aeration in solid-state fermentation (ssf). to study this phenomenon, we determined ros accumulation during lovastati ...201728791446
isolation and characterization of "terrein" an antimicrobial and antitumor compound from endophytic fungus aspergillus terreus (jas-2) associated from achyranthus aspera varanasi, india.the present study aimed at characterizing biological potentials of endophyte aspergillus terreus jas-2 isolated from achyranthus aspera. crude extracted from endophytic fungus jas-2 was purified and chemically characterized by chromatographic and spectroscopic studies respectively. spectral assignment of nmr (nuclear magnetic resonance) data, (1)h proton and (13)c carbon analysis along with ftir data elucidated the structure of compound as 4,5-dihydroxy-3-(1-propenyl)-2-cyclopenten-1-one. after ...201728790982
optimization of submerged fermentation medium for matrine production by aspergillus terreus, an endophytic fungus harboring seeds of sophora flavescens, using response surface methodology.different endophytes isolated from the seeds of sophora flavescens were tested for their ability to produce matrine production. response surface methodology (rsm) was applied to optimize the medium components for the endophytic fungus. results indicated that endophyte aspergillus terreus had the ability to produce matrine. the single factor tests demonstrated that potato starch was the best carbon source and the combination of peptone and nh4no3 was the optimal nitrogen source for a. terreus. th ...201728781541
microbial transformation of artemisinin by aspergillus terreus.artemisinin (1) and its derivatives are now being widely used as antimalarial drugs, and they also exhibited good antitumor activities. so there has been much interest in the structural modification of artemisinin and its derivatives because of their effective bioactivities. the microbial transformation is a promising route to obtain artemisinin derivatives. the present study focuses on the microbial transformation of artemisinin by aspergillus terreus.201728775933
oxidative stress response tips the balance in aspergillus terreus amb resistance.in this study we characterize the impact of antioxidative enzymes in amb resistant (atr) and rare susceptible (ats) clinical a. terreus isolates. we elucidate the expression profiles of superoxide dismutases (sods) and catalases (cats) encoding genes, enzymatic activity of sods, superoxide anion production and signaling pathways involved in oxidative stress response (osr) under amb treatment in ats and atr strains. we show that atr possess almost doubled basal sod activity compared to ats and th ...201728739793
successful medical treatment of an aspergillus terreus mycetoma of the nostril/lip in a 16-year-old fjord pony gelding with pituitary pars intermedia dysfunction.mycetoma is a chronic, proliferative lesion of cutaneous/subcutaneous tissue characterized by draining tracts and granules in the discharge caused by actinomycetes (actinomycetoma) or filamentous fungi (eumycotic mycetoma).201728736852
reply to the comment on "melanisation of aspergillus terreus-is butyrolactone i involved in the regulation of both dopa and dhn types of pigments in submerged culture? microorganisms 2017, 5, 22".n/a.201728677647
proteome profile of aspergillus terreus conidia at germinating stage: identification of probable virulent factors and enzymes from mycotoxin pathways.aspergillus terreus is an emerging opportunistic fungal pathogen that causes invasive aspergillosis in immunocompromised individuals. the main risk group of individuals for this organism is leukopenic patients, individuals having cancers, bone marrow transplant persons and those who have immunological disorders. the lack of early diagnostic marker for a. terreus and intrinsic resistance to amphotericin b, further limits the successful therapy of a. terreus-associated infections. the germination ...201728647921
citreoviridin induces triglyceride accumulation in hepatocytes through inhibiting ppar-α in vivo and in vitro.citreoviridin (cit) is a mycotoxin produced by penicillum citreonigrum, aspergillus terreus and eupenicillium ochrosalmoneum. cit occurs naturally in moldy rice and corn. cit is associated with the development of atherosclerosis in the general population. alteration in hepatic lipid metabolism is a pathogenic factor in atherosclerosis. however the effect and the underlying mechanism of cit on hepatic lipid metabolism are largely unknown. in this study, we reported that cit induced triglyceride a ...201728645467
improving thermostability of (r)-selective amine transaminase from aspergillus terreus through introduction of disulfide bonds.to improve the thermostability of (r)-selective amine transaminase from aspergillus terreus (at-ata), we used computer software disulfide by design and modelling of disulfide bonds in proteins to identify mutation sites where the disulfide bonds were most likely to form. we obtained three stabilized mutants (n25c-a28c, r131c-d134c, m150c-m280c) from seven candidates by site-directed mutagenesis. compared to the wild type, the best two mutants n25c-a28c and m150c-m280c showed improved thermal sta ...201728639260
comment on: "melanisation of aspergillus terreus-is butyrolactone i involved in the regulation of both dopa and dhn types of pigments in submerged culture? microorganisms 2017, 5, 22".a recent article by palonen et al. describes the effect of butyrolactone i on the expression of a secondary metabolite biosynthesis gene cluster from aspergillus terreus that shows similarities to fusarubin biosynthesis gene clusters from fusarium species. the authors claim that two different types of pigments are formed in aspergillus terreus conidia, whereby one pigment is termed a dopa-type melanin and the second a dhn-type melanin. unfortunately, the terminology of the classification of mela ...201728635676
single-step production of the simvastatin precursor monacolin j by engineering of an industrial strain of aspergillus terreus.monacolin j is a key precursor for the synthesis of simvastatin (zocor), an important drug for treating hypercholesterolemia. industrially, monacolin j is manufactured through alkaline hydrolysis of lovastatin, a fungal polyketide produced by aspergillus terreus. multistep chemical processes for the conversion of lovastatin to simvastatin are laborious, cost expensive and environmentally unfriendly. a biocatalysis process for monacolin j conversion to simvastatin has been developed. however, dir ...201728619444
bio-mining the forest ecosystem of north east india for identification of antimicrobial metabolites from fungi through submerged fermentation.in this study, fungi isolated from less explored forest soil ecosystem of northeast india were studied for the production of potential antimicrobial metabolites (amm). out of the 68 fungi isolated from forest soil of manipur, 7 of them showed ama against the test pathogens. among them, aspergillus terreus (ibsd-f4) showed the most significant activity against staphylococcus aureus (atcc-25923), bacillus anthracis (ibsd-c370), pseudomonas fluorescens (atcc-13525), salmonella typhimurium (atcc-140 ...201728578806
glycoside hydrolase production by aspergillus terreus cm20 using mixture design approach for enhanced enzymatic saccharification of alkali pretreated paddy straw.a successful lignocellulosic ethanol production process needs to address the technological impediments such as cost-competitiveness and sustainability of the process. effective biomass utilization requires a repertoire of enzymes including various accessory enzymes. developing an enzyme preparation with defined hydrolytic activities can circumvent the need for supplementing cellulases with accessory enzymes for enhanced hydrolysis. with this objective, mixture design approach was used in the pre ...201628577515
production of itaconic acid by biotransformation of wheat bran hydrolysate with aspergillus terreus cicc40205 mutant.the replacement of the carbon source in the microbial production of itaconic acid (ia) with economic alternatives has attracted significant attention. in this study, an aspergillus terreus cicc40205 mutant was used to increase the ia titer and decrease the citric acid titer in the wheat bran hydrolysate compared with the parental strain. the results showed that the ia titer was increased by 33.4%, whereas the citric acid titer was decreased by 75.8%, and were in accordance with those of the impr ...201728550772
luliconazole, an alternative antifungal agent against aspergillus terreus.aspergillus terreus is the fourth leading cause of invasive and non-invasive aspergillosis and one of the causative agents of morbidity and mortality among immunocompromised and high-risk patients. a. terreus appears to have increased as a cause of opportunistic fungal infections from superficial to serious invasive infections. although, invasive aspergillosis is often treated empirically with amphotericin b, most a. terreus isolates are resistant both in vivo and in vitro to some antifungal dru ...201728483449
melanisation of aspergillus terreus-is butyrolactone i involved in the regulation of both dopa and dhn types of pigments in submerged culture?pigments and melanins of fungal spores have been investigated for decades, revealing important roles in the survival of the fungus in hostile environments. the key genes and the encoded enzymes for pigment and melanin biosynthesis have recently been found in ascomycota, including aspergillus spp. in aspergillus terreus, the pigmentation has remained mysterious with only one class of melanin biogenesis being found. in this study, we examined an intriguing, partially annotated gene cluster of a. t ...201728471414
terrein performs antitumor functions on esophageal cancer cells by inhibiting cell proliferation and synergistic interaction with cisplatin.terrein is a bioactive fungal metabolite isolated from aspergillus terreus. besides being a melanogenesis inhibitor, previous studies have revealed that terrein has antiproliferative effects on a number of types of cancer tumors. in the present study, the inhibitory effect of terrein on esophageal cancer was evaluated and the possible underlying mechanisms were investigated. the results revealed that terrein inhibited the proliferation of eca109 esophageal cancer cells in a dose- and time-depend ...201728454470
process relevant screening of cellulolytic organisms for consolidated bioprocessing.although the biocatalytic conversion of cellulosic biomass could replace fossil oil for the production of various compounds, it is often not economically viable due to the high costs of cellulolytic enzymes. one possibility to reduce costs is consolidated bioprocessing (cbp), integrating cellulase production, hydrolysis of cellulose, and the fermentation of the released sugars to the desired product into one process step. to establish such a process, the most suitable cellulase-producing organis ...201728450887
production of itaconic acid from pentose sugars by aspergillus terreus.itaconic acid (ia), an unsaturated 5-carbon dicarboxylic acid, is a building block platform chemical that is currently produced industrially from glucose by fermentation with aspergillus terreus. however, lignocellulosic biomass has potential to serve as low-cost source of sugars for production of ia. research needs to be performed to find a suitable a. terreus strain that can use lignocellulose-derived pentose sugars and produce ia. one hundred a. terreus strains were evaluated for the first ti ...201728440059
new approaches for itaconic acid production: bottlenecks and possible remedies.currently, growing attention is being devoted to the conversion of biomass into value-added products, such as itaconic acid (ia), which is considered as the cleanest alternative to petroleum-based acrylic acid. ia is an unsaturated dicarboxylic acid that is used as a building block chemical for the production of several value-added products such as poly-itaconic acid. ia and its derivatives have a wide range of potential applications in textile, paint, pharmaceutical and chemical industries. pre ...201728425297
a new approach to models of the 4,5-dihydroxycyclopentenone core of the kodaistatins a-d: elucidation of the diol configuration in kodaistatin a.the kodaistatins a-d are strongly anti-diabetic natural products from aspergillus terreus that hold some promise of a novel diabetes cure. however, considerations of that kind face two drawbacks: 1) the kodaistatins a-d contain a heavily substituted pulvinone/cyclopentenone combination; 2) they are 1,2-diols, the 3d structures of which have not been assigned yet. however, we can exclude two of the four possible stereostructures. we conclude that kodaistatin a is a trans-, not a cis-diol from nmr ...201728421637
a prospective international aspergillus terreus survey: an efisg, isham and ecmm joint study.a prospective international multicentre surveillance study was conducted to investigate the prevalence and amphotericin b susceptibility of aspergillus terreus species complex infections.201728412383
fermentative production and optimization of mevastatin in submerged fermentation using aspergillus terreus.the main objective of the study is to enhance the mevastatin production using plackett-burman (pb) and central composite design (ccd) by aspergillus terreus in submerged fermentation (smf). eight nutrients were chosen for a pb design with 12 experimental runs. a maximum mevastatin production of 170.4 mg l(-1) was obtained in pb design. response surface methodology (rsm) is a sequential procedure with an initial objective to lead the experimenter rapidly and efficiently along a path of improvemen ...201528435810
gentamicin degradation and changes in fungal diversity and physicochemical properties during composting of gentamicin production residue.an indoor co-composting of gentamicin fermentation residues (gfr) and lovastatin fermentation residues (lfr) inoculated with gentamicin-degrading aspergillus terreus fzc3 was conducted to remove gentamicin residues. the results showed that treatment mfzc3, consisting of a 10:1 blend of gfr and lfr (w/w), had the longest thermophilic phase (7days), quickest gentamicin degradation (t½=4.4days), and relatively higher gentamicin degradation percentage (96.7%) at the end of composting. addition of as ...201728847079
periprosthetic hip joint infection with aspergillus terreus: a clinical case and a review of the literature.fungal periprosthetic joint infections due to aspergillus species are rare but are associated with significant cost and morbidity. we present a case of asperigillus terreus prosthetic joint infection of the hip. the patient was successfully treated with a prolonged course of systemic antifungals along with surgical management.201728808616
absence of subchronic oral toxicity and genotoxicity of rice koji with aspergillus terreus.koji products have been considered as an effective fermented food consumed in east asia with many health benefits. particularly, rice koji with aspergillus terreus (rat) has been reported to be able to prevent hyperlipidemia and hepatic steatosis through regulating cholesterol synthesis. despite its biological activities, there is a lack of comprehensive information to give an assurance of its safety. therefore, the objective of this study was to perform a series of toxicological studies (repeat ...201728802559
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