Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| use of high inoculum for early metabolic signalling and rapid susceptibility testing of aspergillus species. | to develop and evaluate a new method for rapid susceptibility testing of aspergillus spp. based on early metabolic signalling of high-inoculum biomass. | 2007 | 17185296 |
| biodegradation of crude oil and n-alkanes by fungi isolated from oman. | ten fungal species isolated from tar balls collected from the beaches of oman were tested for their abilities to grow and degrade n-alkanes and crude oil. the abilities of aspergillus niger, a. ochraceus and penicillium chrysogenum to degrade n-alkanes (c13-c18), crude oil were compared and their mycelial biomass was measured. significant differences were found in the utilization of c15, c16, c17 and c18 by the three fungi. similarly, significant differences we found in the amount of biomass pro ... | 2007 | 17904586 |
| characterization of xylanase from lentinus edodes m290 cultured on waste mushroom logs. | extracellular enzymes from lentinus edodes m290 on normal woods (quercus mongolica) and waste logs from oak mushroom production were comparatively investigated. endoglucanase, cellobiohydrolase, beta-glucosidase, and xylanase activities were higher on waste mushroom logs than on normal woods after l. edodes m290 inoculation. xylanase activity was especially different, with a three times higher activity on waste mushroom logs. when the waste mushroom logs were used as a carbon source, a new 35 kd ... | 2007 | 18092465 |
| the effect of antifungal agents and human monocytes on in vitro galactomannan release by aspergillus spp. in liquid culture medium. | invasive pulmonary aspergillosis is increasing in incidence in immunosuppressed patients. diagnosis of this infection is problematic, relying on clinical suspicion and computerized tomography of the thorax and sinuses. an assay capable of detecting the fungal cell wall component galactomannan (gm) as a sign of aspergillus infection is in use in patients with hematological malignancies. the aim of this study was to investigate the release of gm during growth of two medically important species, as ... | 2007 | 18184406 |
| a 7-dimethylallyltryptophan synthase from aspergillus fumigatus: overproduction, purification and biochemical characterization. | a putative prenyltransferase gene, afu3g12930, was identified in the genome sequence of aspergillus fumigatus. eal92290, encoded by afu3g12930, consists of 472 aa, with a molecular mass of about 53 kda. the coding sequence of afu3g12930 was cloned in pqe60, and overexpressed in escherichia coli. the soluble his(6)-fusion protein was purified to apparent homogeneity, and characterized biochemically. the enzyme was found to catalyse the prenylation of trp at the c-7 position of the indole moiety, ... | 2007 | 17906140 |
| in vitro determination of phagocytosis and intracellular killing of aspergillus species by mononuclear phagocytes. | we investigated phagocytosis and intracellular killing of clinical and environmental isolates of aspergillus spp. by human monocyte-derived macrophages (mdms). serial pathogens such as aspergillus fumigatus, aspergillus flavus and aspergillus terreus were examined with a microbiological assay. phagocytosis for resting conidia of aspergillus spp. was similar for all isolates tested. during 30 min of incubation phagocytosis ranged from 49.9% to 85.5% for clinical isolates and from 40.3% to 87.1% f ... | 2007 | 17458710 |
| voriconazole in the management of nosocomial invasive fungal infections. | voriconazole is a new triazole developed for the treatment of life-threatening fungal infections. the drug is available for both oral and intravenous administration; the oral formulation has excellent bioavailability. the side-effect profile of voriconazole presents an acceptable safety and tolerability spectrum: transient visual disturbances, liver enzyme abnormalities, and skin rashes are the most frequently reported side effects but rarely lead to discontinuation. the potential for drug-drug ... | 2006 | 18360588 |
| antifungal interactions within the triple combination of amphotericin b, caspofungin and voriconazole against aspergillus species. | the in vitro effects of caspofungin combined with voriconazole and amphotericin b were tested in triplicate experiments against nine clinical isolates of aspergillus fumigatus, aspergillus flavus and aspergillus terreus. | 2006 | 17071635 |
| stimulation of alkalothermophilic aspergillus terreus xylanase by low-intensity laser radiation. | in this study, aspergillus terreus was irradiated by a 7.3 mw he-ne laser in the presence of crystal violet, toluidine blue o and hematoporphyrin as photosensitizers. xylanases recovered from non-irradiated and irradiated fungi were purified and characterized. the maximum production of xylanase (42.2 u/ml) was obtained after 5 min of laser irradiation in the absence of the photosensitizer. the irradiation of the sensitized fungus diminished the production of xylanase. on purification using g-100 ... | 2006 | 16810525 |
| exposure to airborne fungi during conservative dental treatment. | the aim of the study was a mycological assessment of bioaerosol forming during conservative dental treatment, taking into account concentration and type of fungal microflora, and evaluation of the influence of duwl disinfecting protocol on the fungal contamination of the bioaerosol. the research was conducted on 25 operative sites located in public dental clinics. the air contained in the space between a patient and a dentist during conservative dental treatment with the use of a high-speed hand ... | 2006 | 16841889 |
| comparison of the eucast-afst broth dilution method with the clsi reference broth dilution method (m38-a) for susceptibility testing of posaconazole and voriconazole against aspergillus spp. | the susceptibilities of 40 clinical isolates of aspergillus spp. (aspergillus fumigatus, aspergillus flavus, aspergillus niger, aspergillus terreus) were determined for posaconazole and voriconazole by the clsi m38-a and eucast-afst broth dilution methods. where a discrepancy was observed between the methods, the eucast method tended to give higher mic values. overall, the level of agreement was 92.5% and the intra-class correlation coefficient was > 0.90. | 2006 | 16882296 |
| in-vitro susceptibility testing by agar dilution method to determine the minimum inhibitory concentrations of amphotericin b, fluconazole and ketoconazole against ocular fungal isolates. | to standardize in-vitro antifungal susceptibility testing by agar dilution method to find out the minimum inhibitory concentration (mic) of amphotericin b, fluconazole and ketoconazole on ocular fungal isolates. | 2006 | 17185846 |
| processing of soybean products by semipurified plant and microbial alpha-galactosidases. | galactooligosaccharides (go) are responsible for intestinal disturbances following ingestion of legume-derived products. enzymatic reduction of go level in these products is highly desirable to improve their acceptance. for this purpose, plant and microbial semipurified alpha-galactosidases were used for go hydrolysis in soybean flour and soy molasses. alpha-galactosidases from soybean germinating seeds, aspergillus terreus, and penicillium griseoroseum presented maximal activities at ph 4.0-5.0 ... | 2006 | 17177558 |
| susceptibility of fungi isolated from the respiratory tract of falcons to amphotericin b, itraconazole and voriconazole. | the minimum inhibitory concentrations (mics) of fungi isolated from the air sacs of falcons before (group 1), and during antifungal treatment with amphotericin b nebulisation and oral itraconazole or voriconazole (group 2), or with itraconazole alone (group 3) or voriconazole alone (group 4) were determined. before treatment, 95 per cent of the isolates, including aspergillus fumigatus, aspergillus flavus, aspergillus niger and aspergillus terreus, were susceptible to voriconazole at mics up to ... | 2006 | 16946311 |
| analysis of subunit interactions in the iterative type i polyketide synthase atx from aspergillus terreus. | 2006 | 17004275 | |
| efficacy of caspofungin against aspergillus flavus, aspergillus terreus, and aspergillus nidulans. | the echinocandin caspofungin is a potent inhibitor of the activity of 1,3-beta-d-glucan synthase from aspergillus flavus, aspergillus terreus, and aspergillus nidulans. in murine models of disseminated infection, caspofungin prolonged survival and reduced the kidney fungal burden. caspofungin was at least as effective as amphotericin b against these filamentous fungi in vivo. | 2006 | 17015628 |
| hexadecylphosphocholine (miltefosine) has broad-spectrum fungicidal activity and is efficacious in a mouse model of cryptococcosis. | the alkyl phosphocholine drug miltefosine is structurally similar to natural substrates of the fungal virulence determinant phospholipase b1 (plb1), which is a potential drug target. we determined the mics of miltefosine against key fungal pathogens, correlated antifungal activity with inhibition of the plb1 activities (plb, lysophospholipase [lpl], and lysophospholipase-transacylase [lpta]), and investigated its efficacy in a mouse model of disseminated cryptococcosis. miltefosine inhibited sec ... | 2006 | 16436691 |
| enzymatic characteristics of ligninperoxidases from penicillium citrinum, fusarium oxysporum and aspergillus terreus using n-propanol as substrate. | the activities of ligninperoxidases from penicillium citrinum mtcc 3565, fusarium oxysporum mtcc 3379 and aspergillus terreus mtcc 3374 have been assayed and the enzymatic characteristics like km, ph and temperature optima using n-propanol as the substrate have been reported. the results suggest that n-propanol can substitute veratryl alcohol as substrate for assaying ligninperoxidase activities from different fungal strains, without affecting the enzymatic characteristics. the above strains wer ... | 2006 | 16955752 |
| antifungal efficacy and pharmacodynamics of posaconazole in experimental models of invasive fungal infections. | posaconazole is a novel lipophilic antifungal triazole with potent and broad-spectrum activity against opportunistic, endemic and dermatophytic fungi. this activity extends to organisms that are often refractory to existing triazoles, amphotericin b or echinocandins such as candida glabrata, candida krusei, aspergillus terreus, fusarium spp. and the zygomycetes. a large number of experimental animal models of invasive fungal infections has demonstrated the potent and broad-spectrum efficacy of p ... | 2006 | 16961576 |
| early detection of aspergillus terreus in a tenckhoff catheter. | 2006 | 17047247 | |
| cloning, expression, and characterization of a xylanase 10 from aspergillus terreus (bcc129) in pichia pastoris. | a full-length xylanase gene, encoding 326 amino acids belonging to the fungal glycosyl hydrolase family 10, from aspergillus terreus bcc129 was cloned and sequenced. sequence analysis suggested that the first 25 amino acids of this enzyme is the signal peptide. therefore, only the mature xylanase gene of 906 bp was cloned into a yeast expression vector, ppiczalphaa, for heterologous expression in pichia pastoris. a band of approximately, 33 kda was observed on the sds-page gel after one day of m ... | 2006 | 16275128 |
| intrinsic in vitro susceptibility of primary clinical isolates of aspergillus fumigatus, aspergillus terreus, aspergillus nidulans, candida albicans and candida lusitaniae against amphotericin b. | a total of 60 clinical fungal isolates from patients without prior amphotericin b treatment and three control strains were evaluated for their intrinsic susceptibility to amphotericin b (amb) using microdilution, etest and disc diffusion assays, on three media each, roswell park memorial institute (rpmi) 1640, antibiotic medium 3 (am3) and high resolution medium. the fungal strains included isolates of aspergillus fumigatus (n = 10), aspergillus terreus (n = 12), aspergillus nidulans (n = 9), ca ... | 2006 | 16466441 |
| disseminated aspergillosis in two dogs in israel. | aspergillus terreus, normally a soil or plant saprophyte, causes disseminated systemic infection, involving primarily the skeletal and the cardiopulmonary system in humans and dogs.(1, 2) we describe two cases of german shepherd dogs that were referred to koret school of veterinary medicine teaching hospital with a history of anorexia and weakness. case 1 suffered from neurological deficits, paraparesis and lumbar pain whereas case 2 suffered from unilateral uveitis and exophthalmus. both dogs w ... | 2006 | 16466447 |
| effects of the sporulation conditions on the lovastatin production by aspergillus terreus. | the production of biomass and lovastatin by spore-initiated submerged fermentations of aspergillus terreus atcc 20542 was shown to depend on the age of the spores used for inoculation. cultures started from older spores produced significantly higher titers of lovastatin. for example, the lovastatin titer increased by 52% when the spore age at inoculation rose from 9 to 16 days. the lovastatin titer for a spore age of 16 days was 186.5+/-20.1 mg l(-1). the time to sporulation on surface cultures ... | 2006 | 16491374 |
| functional characterization of alcohol oxidases from aspergillus terreus mtcc 6324. | short chain alcohol oxidase (scao), long chain alcohol oxidase (lcao), secondary alcohol oxidase (sao), and aryl alcohol oxidase (aao) activities were localized in the microsome of aspergillus terreus during growth of the fungi on n-hexadecane. zymogram analysis of the microsomes of n-hexadecane-grown cells in polyacrylamide gel electrophoresis showed distinct bands, h4, h3, h2, and h1, in a sequence of their molecular weight (mr) from high to low. the mr of the isozymes corresponding to the ban ... | 2006 | 16547701 |
| antimicrobial, wound healing and antioxidant activity of plagiochasma appendiculatum lehm. et lind. | plagiochasma appendiculatum (aytoniaceae) of the order marchantiales is widely used in the form of paste ethnomedicinally by gaddi tribe in kangra valley for treating skin diseases. in this context, antimicrobical potential of plagiochasma appendiculatum against a wide range of microorganisms was studied. to validate the ethnotherapeutic claims of the plant in skin diseases, wound healing activity was studied, besides antioxidant activity to understand the mechanism of wound healing activity. th ... | 2006 | 16600543 |
| raw starch degrading amylase production by various fungal cultures grown on cassava waste. | the solid waste of sago industry using cassava was fermented by aspergillus niger, aspergillus terreus and rhizopus stolonifer in solid state fermentation. cassava waste contained 52 per cent starch and 2.9 per cent protein by dry weight. the amylase activity was maintained at a high level and the highest amylase activity was observed on the 8(th) day in r. stolonifer mediated fermentation. r. stolonifer was more efficient than aspergillus niger and aspergillus terreus in bioconverting cassava w ... | 2006 | 24039485 |
| bioconversion of straw into improved fodder: mycoprotein production and cellulolytic acivity of rice straw decomposing fungi. | sixty two out of the sixty four species of fungal isolates tested could produce both exo-β1,4-gluconase (c1) and endo-β1,4-gluconase (cx) on pure cellulose and rice straw as carbon source in czapek's medium. fifty-eight and fifteen species were able to grow at 25℃ and at 45℃, respectively. eleven species could grow at both 25℃ and 45℃ while, four species appeared only at 45℃. the most cellulolytic species at 25℃ was trichoderma koningii producing 1.164 c1 (mg glucose/1 ml culture filtrate/1 hr) ... | 2005 | 24049480 |
| total structural assignment and absolute configuration of terreinol by 13c and 1h nmr. | the carbon-carbon connectivity of terreinol, a new metabolite isolated from aspergillus terreus, and its previous (13)c assignments were confirmed by a two-dimensional inadequate experiment using a few milligrams of the compound with natural (13)c abundance. the carbon-carbon correlations were determined by computational analysis (with >99% probability) of this experiment. additionally, the absolute configuration of terreinol was achieved indirectly via its corresponding secondary alcohol by the ... | 2005 | 15625725 |
| bioremediation of endosulfan using aspergillus terreus and cladosporium oxysporum. | 2005 | 16400595 | |
| efficacy of caspofungin against aspergillus terreus. | we investigated the in vitro and in vivo activities of caspofungin against aspergillus terreus. the drug increased survival and reduced tissue fungal burden in neutropenic mice. therefore, our data support the role of caspofungin in treating systemic infections due to this emerging pathogen. | 2005 | 16304185 |
| analysis of the influence of tween concentration, inoculum size, assay medium, and reading time on susceptibility testing of aspergillus spp. | the influence of several test variables on susceptibility testing of aspergillus spp. was assessed. a collection of 28 clinical isolates was tested against amphotericin b, itraconazole, voriconazole, and terbinafine. inoculum size (10(4) cfu/ml versus 10(5) cfu/ml) and glucose supplementation (0.2% versus 2%) did not have significant effects on antifungal susceptibility testing results and higher inoculum size and glucose concentration did not falsely elevate mics. in addition, antifungal suscep ... | 2005 | 15750092 |
| studies on the application of immobilization technique for the production of cyclosporin a by a local strain of aspergillus terreus. | the formation of cyclosporin a (cy a) by immobilized spores and mycelia of aspergillus terreus was investigated. different carriers were tested as immobilizing carriers, whereby ca-alginate was selected for further experimentation. the role of alginate concentration, biomass weight, ph value of the cultivation medium, repeated utilization of the immobilized fungus as well as the supplementation of different amino acid precursors were studied. best cy a outputs were attained with ca-alginate 3% ( ... | 2005 | 16107751 |
| sesterterpenoids, terretonins a-d, and an alkaloid, asterrelenin, from aspergillus terreus. | four new sesterterpenoids, terretonins a-d (1-4), and a new alkaloid, asterrelenin (5), together with five known compounds, were isolated from the ethyl acetate extract of a solid-state fermented culture of aspergillus terreus. their structures were elucidated on the basis of spectroscopic analysis. the structures of 1, 2, and 5 were confirmed by x-ray crystallographic analysis. | 2005 | 16124769 |
| nad deamidation "a new reaction" by an enzyme from aspergillus terreus dsm 826. | nad deamidation is a non-previously recognized reaction. this reaction has been found to be catalyzed by extracts of aspergillus terreus dsm 826. conversion of nad to the biosynthetic intermediate, deamido nad, by these extracts, at the optimum ph and temperature did not exceed about 55 of the amount of the substrate added. completion of the reaction was achieved when the extracts were pre-heated at 50 degrees c for 15 min in absence of the substrate. in a very similar manner, the extracts catal ... | 2005 | 15793621 |
| amphotericin b lipid complex in the management of invasive aspergillosis in immunocompromised patients. | the efficacy and renal safety of amphotericin b lipid complex (ablc) were assessed in 398 patients with invasive aspergillosis. the most common underlying conditions were hematopoietic stem-cell transplantation (101/398 [25%]), hematologic malignancy (101/398 [25%]), and solid-organ transplantation (109/398 [27%]). the most common reason for administration of ablc was lack of response to prior antifungal therapy. overall, 65% of patients had a favorable clinical response: 44% were cured or impro ... | 2005 | 15809925 |
| non-dermatophytic molds as agents of onychomycosis in izmir, turkey - a prospective study. | the purpose of this study was to determine the prevalence of causative non-dermatophytic filamentous fungi in onychomycosis. totally 1,222 (1,222 x 3 = 3,666) samples of nail scrapings from 1,146 patients (from 76 patients two specimens: both from finger- and toe-nails) with prediagnosis of onychomycosis sent to the mycology laboratory from the clinic of dermatology, ege university hospital, izmir, turkey, july 2001-december 2003, were prospectively studied with conventional mycological procedur ... | 2005 | 16170607 |
| in vitro activities of amphotericin b and voriconazole against aleurioconidia from aspergillus terreus. | this study used aleurioconidia as inoculum and compared the mics of amphotericin b and voriconazole to those obtained for conidia of 31 aspergillus terreus strains. for conidia and aleurioconidia, the mic at which 90% of strains were inhibited was 2.5 microg/ml and 5 microg/ml with amphotericin b and 1 microg/ml and 2 microg/ml with voriconazole. | 2005 | 15917567 |
| aspergillus terreus endophthalmitis. | we report a case of aspergillus terreus endophthalmitis and review the 4 previously reported cases. immunosuppression, intravenous drug use, intraocular surgery or trauma, and a compatible clinical picture should raise suspicion of the diagnosis. this species often exhibits resistance to amphotericin b and outcomes are poor despite current therapies. | 2005 | 16012022 |
| occurrence of fructosyl-amino acid oxidase-reactive compounds in fungal cells. | fructosyl-amino acid oxidase (faod)-reactive fraction (fry) was found in commercial yeast extract. fry showed very hydrophilic property and was adsorbed to phenylboronate silica gel, indicating that it contained the amadori compound. tlc and amino acid analyses revealed that glucosone, lysine, and arginine were produced from fry after incubation with faod. tof-ms analysis confirmed that fry is a mixture of fructosyl lysine and fructosyl arginine. these compounds were also detected in mycelial ex ... | 2005 | 15665502 |
| micafungin in combination with voriconazole in aspergillus species: a pharmacodynamic approach for detection of combined antifungal activity in vitro. | preclinical and clinical evidence indicate that echinocandin-triazole combinations provide enhanced killing versus triazoles alone against some aspergillus isolates, however, in vitro test results designed to detect this combined effect are difficult to interpret. | 2005 | 16188916 |
| posaconazole: clinical pharmacology and potential for management of fungal infections. | posaconazole is a novel lipophilic antifungal triazole that inhibits cytochrome p450-dependent 14-alpha demethylase in the biosynthetic pathway of ergosterol. inhibition of this enzyme leads to an accumulation of toxic 14-alpha methylsterols and a depletion of ergosterol, resulting in a perturbation of the function of the fungal cell membrane and blockage of cell growth and division. in vitro, posaconazole has potent and broad-spectrum activity against opportunistic, endemic and dermatophytic fu ... | 2005 | 16107193 |
| development of a dna microarray for detection and identification of fungal pathogens involved in invasive mycoses. | invasive fungal infections have emerged as a major cause of morbidity and mortality in immunocompromised patients. conventional identification of pathogenic fungi in clinical microbiology laboratories is time-consuming and, therefore, often imperfect for the early initiation of an adequate antifungal therapy. we developed a diagnostic microarray for the rapid and simultaneous identification of the 12 most common pathogenic candida and aspergillus species. oligonucleotide probes were designed by ... | 2005 | 16207946 |
| assessment of ribosomal large-subunit d1-d2, internal transcribed spacer 1, and internal transcribed spacer 2 regions as targets for molecular identification of medically important aspergillus species. | molecular approaches are now being developed to provide a more rapid and objective identification of fungi compared to traditional phenotypic methods. ribosomal targets, especially the large-subunit rna gene (d1-d2 region) and internal transcribed spacers 1 and 2 (its1 and its2 regions), have shown particular promise for the molecular identification of some fungi. we therefore conducted an assessment of these regions for the identification of 13 medically important aspergillus species: aspergill ... | 2005 | 15872227 |
| use of fluorescent probes to determine mics of amphotericin b and caspofungin against candida spp. and aspergillus spp. | we investigated the utility of mechanism-based fluorescent probes for determination of mics (fmics) of amphotericin b and caspofungin against candida spp. and aspergillus spp. amphotericin b was selected as a membrane-active antifungal agent, and caspofungin was selected as a cell wall-active agent. fmics were also compared to the mic determined by clsi (formerly nccls) methods. five isolates per species of candida albicans, candida glabrata, candida parapsilosis, aspergillus fumigatus, and aspe ... | 2005 | 16081911 |
| the anticancer activity of the fungal metabolite terrecyclic acid a is associated with modulation of multiple cellular stress response pathways. | tumors are dependent on cellular stress responses, in particular the heat shock response, for survival in their hypoxic, acidotic, and nutrient-deprived microenvironments. using cell-based reporter assays, we have identified terrecyclic acid a (tca) from aspergillus terreus, a fungus inhabiting the rhizosphere of opuntia versicolor of the sonoran desert, as a small-molecule inducer of the heat shock response that shows anticancer activity. further characterization suggested that tca also affects ... | 2005 | 16227407 |
| evolutionary relationships among aspergillus terreus isolates and their relatives. | aspergillus terreus is a ubiquitous fungus in our environment. it is an opportunistic human pathogen and economically important as the main producer of lovastatin, a cholesterol lowering drug. our aim was to examine the genetic variability of a. terreus and closely related species using molecular and analytical techniques. lovastatin production was examined by hplc. lovastatin was produced by seven isolates belonging to the species a. terreus. rapd analyses were carried out using 25 different ra ... | 2005 | 16096690 |
| epidemiology and outcome of infections due to aspergillus terreus: 10-year single centre experience. | aspergillus terreus, a less common pathogen, appears to be an emerging cause of infection at our institution, the medical university hospital of innsbruck. thus the epidemiology and outcome of a. terreus infections over the past 10 years was assessed. we analysed 67 cases of proven invasive aspergillosis (ia) according to the european organisation for research and treatment of cancer/mycoses study group criteria, investigated antifungal susceptibility of amphotericin b (amb), voriconazole and ca ... | 2005 | 16197450 |
| determination of antifungal drug susceptibilities of aspergillus species by a fluorescence-based microplate assay. | we have investigated the use of a viability dye, chloromethylfluorescein di-acetate (cmfda), for antifungal susceptibility testing in a fluorescence microplate (fm) assay format. | 2005 | 15546970 |
| isoterreulactone a, a novel meroterpenoid with anti-acetylcholinesterase activity produced by aspergillus terreus. | a new seven-membered lactone type meroterpenoid, isoterreulactone a, was isolated from the solid state fermentation of aspergillus terreus and its structure was established by various spectral analysis. isoterreulactone a inhibited acetylcholinesterase with an ic(50) value of 2.5 microm while did not inhibit butyrylcholinesterase even at 500 microm. | 2005 | 15603953 |
| novel method for cell immobilization and its application for production of organic acid. | the aim was to develop a novel and simple technique for the entrapment of fungal hyphae. | 2005 | 15715641 |
| parallel capillary electrophoresis for the quantitative screening of fermentation broths containing natural products. | directed molecular evolution is a recursive process of controlled genetic diversification and functional screening. the success of this approach is dependent on both the quality of the genetic diversity and the ability to accurately screen a large population of individual genetic variants for those having improved function. in this paper, the application of parallel capillary electrophoresis to rapidly quantitate lovastatin production levels by aspergillus terreus mutants is described. a paralle ... | 2005 | 15721810 |
| pyrene and benzo(a)pyrene metabolism by an aspergillus terreus strain isolated from a polycylic aromatic hydrocarbons polluted soil. | a strain of aspergillus terreus was isolated from a polycyclic aromatic hydrocarbons (pahs) polluted soil. the metabolism of pyrene and benzo(a)pyrene by this fungus was investigated in liquid submerged culture added of 50 and 25 ppm respectively of each compound. depletion of pyrene and benzo(a)pyrene was evident during the first stages of growth and was 60% and 27.5% respectively of the added amount after nine days of culture. solvent extracts of the fermentation broth and mycelium were analys ... | 2004 | 15068369 |
| rational elimination of aspergillus terreus sulochrin production. | elimination of undesirable co-metabolites from industrial fermentations is often required due to the toxicities associated with the contaminants and/or due to difficulties in removing the contaminants during downstream processing. sulochrin is a co-metabolite produced during the aspergillus terreus lovastatin fermentation. examination of the sulochrin biosynthetic pathway identifies the emodin anthrone polyketide synthase (pks) at the origin. thus, genetically disrupting the emodin anthrone pks ... | 2004 | 15129726 |
| aristolochene synthase: mechanistic analysis of active site residues by site-directed mutagenesis. | incubation of farnesyl diphosphate (1) with penicillium roqueforti aristolochene synthase yielded (+)-aristolochene (4), accompanied by minor quantities of the proposed intermediate (s)-(-)germacrene a (2) and the side-product (-)-valencene (5) in a 94:4:2 ratio. by contrast, the closely related aristolochene synthase from aspergillus terreus cyclized farnesyl diphosphate only to (+)-aristolochene (4). site-directed mutagenesis of amino acid residues in two highly conserved mg(2+)-binding domain ... | 2004 | 15186158 |
| comparison of the sensititre yeastone colorimetric antifungal panel with a modified nccls m38-a method to determine the activity of voriconazole against clinical isolates of aspergillus spp. | the susceptibilities of 63 isolates of aspergillus spp. to voriconazole were evaluated by a modified nccls m38-a method and the sensititre yeastone method. the overall agreement was 82.5%, ranging from 100% for aspergillus niger and aspergillus terreus to 62.5% for aspergillus flavus. discrepancies between the methods were due to higher sensititre mics. the sensititre yeastone method could have potential value for susceptibility testing of aspergillus spp. to voriconazole. | 2004 | 15365044 |
| synthesis and biological evaluation of analogues of butyrolactone i and molecular model of its interaction with cdk2. | a series of analogues of butyrolactone i, a natural product isolated from aspergillus terreus that selectively inhibits the cdk2 and cdk1 kinases and that has been found to exhibit an interesting antiproliferative activity, have been synthesized. its antitumor activity has been tested. molecular models of the complex between butyrolactone i and the cdk2 active site have been built using a combination of conformational search and automated docking techniques. the stability of the resulting comple ... | 2004 | 15227538 |
| a multicenter, open-label clinical study of micafungin (fk463) in the treatment of deep-seated mycosis in japan. | the efficacy and safety of micafungin (fk463), which is a new lipopeptide antifungal agent of the echinocandin class and is active against both aspergillus and candida species, were investigated in patients with deep-seated mycosis in this study. 70 patients were treated with micafungin 12.5-150 mg/d intravenously for up to 56 d. the overall clinical response rates were 60% (6/10) in invasive pulmonary aspergillosis, 67% (6/9) in chronic necrotizing pulmonary aspergillosis, 55% (12/22) in pulmon ... | 2004 | 15287383 |
| fractionation of the naringinase complex from aspergillus terreus by dye affinity chromatography. | affinity chromatography with immobilised triazine dyes was used to separate the main enzymes present in the naringinase complex produced by aspergillus terreus cect 2663. one alpha-l-rhamnosidase and two beta-d-glucosidases (beta g1 and beta g2) were separated by a simple two-step procedure involving chromatography with red he-3b immobilised on sepharose 4b first at ph 5.5 and then at ph 4.7. maximum activity of the beta-d-glucosidases was from ph 4 to 6 and at 65 degrees c. both glucosidases we ... | 2004 | 15483384 |
| aspergillus terreus peritonitis in a capd patient: report of a case. | 2004 | 15104352 | |
| production of polyclonal antibodies against territrem b and detection of territrem b in the conidia of aspergillus terreus 23-1 by immunoelectron microscopy. | territrem b, a fungal metabolite isolated from aspergillums terreus 23-1, is a tremorgenic mycotoxin. immunoelectron microscopy using anti-territrem b polyclonal antibody was used to detect territrem b in the fungal body of a. terreus 23-1 at different times of culture without shaking on potato dextrose (pd) agar medium. the anti-territrem b serum was produced by immunization of rabbits with 4beta-hydroxymethyl-4beta-demethylterritrem b-sccinate bound by a linker to keyhole limpet hemocyanin. th ... | 2004 | 15161198 |
| receptor assay-guided isolation of anti-gabaergic insecticidal alkaloids from a fungal culture. | the gamma-aminobutyric acid (gaba) receptor bears sites of action for insecticides. to discover gaba receptor-directed insecticides in natural products, fungal culture extracts were screened for their ability to inhibit specific binding of the radiolabeled noncompetitive antagonist [3h]1-(4-ethynylphenyl)-4-n-propyl-2,6,7-trioxabicyclo[2.2.2]octane to housefly head membranes. the screening efforts led to the isolation of two alkaloids from aspergillus terreus: pf1198a (alantrypinone) and pf1198b ... | 2004 | 15186111 |
| 4-hydroxykigelin and 6-demethylkigelin, root growth promoters, produced by aspergillus terreus. | root growth promoters, 4-hydroxykigelin (1) and 6-demethylkigelin (2), together with 6-hydroxymellein (3) were isolated from cultures of the fungus aspergillus terreus and their structures were identified by spectroscopic analysis. the biological activities of the three dihydroisocoumarins, 1, 2, and 3, have been examined using a bioassay method with lettuce seedlings. furthermore, interactions between the dihydroisocoumarins and indole-3-acetic acid against the root growth have been examined. | 2004 | 15241930 |
| intracellular expression of vitreoscilla hemoglobin in aspergillus terreus to alleviate the effect of a short break in aeration during culture. | a continuous supply of o(2) is important for itaconic acid production in aspergillus terreus. any interruption of aeration significantly reduces itaconic acid production. to overcome this effect, a. terreus m8 was transformed with the vitreoscilla hemoglobin gene (vgb) which, as shown by southern hybridization, was integrated into the recipient chromosome. the activity of the expressed hemoglobin was confirmed by a co-difference spectrum. during itaconic acid production, the effect of a break in ... | 2004 | 15218381 |
| in vitro analyses, animal models, and 60 clinical cases of invasive aspergillus terreus infection. | 2004 | 15328076 | |
| lovastatin inhibits its own synthesis in aspergillus terreus. | lovastatin suppresses its own synthesis in the microfungus aspergillus terreus. the inhibitory effect was documented by spiking identical batch cultures with pure lovastatin (0, 50, 100 and 250 mg/l) 24 h after initiation from spores. | 2004 | 14740252 |
| clinical identification of common species of dermatophytes by pcr and pcr-rflp. | to find a fast and efficient way of identifying seven common dermatophytes in clinical practice, we used the techniques of polymerase chain reaction (pcr) and pcr-restriction fragment length polymorphism (rflp) targeting topoisomerase ii gene. the dna of 7 dermatophytes, along with candida albicans, aspergillus terreus and aspergillus flavus were amplified by consensus primer dpsd1. they were then subjected to a second pcr with primers dpsd2 and species-specific primers pst and psme separately. ... | 2004 | 15791867 |
| infections due to aspergillus terreus: a multicenter retrospective analysis of 83 cases. | current in vitro and in vivo data indicate that invasive aspergillosis due to aspergillus terreus is resistant to treatment with amphotericin b. because little clinical data are available to guide therapy, we performed a retrospective cohort study of cases of invasive a. terreus infections from 1997-2002 to determine whether the use of voriconazole, compared with use of other antifungal therapies, led to an improved patient outcome. we analyzed a total of 83 cases of proven or probable invasive ... | 2004 | 15307028 |
| aspergillus terreus: an emerging amphotericin b-resistant opportunistic mold in patients with hematologic malignancies. | invasive aspergillosis (ia) has emerged as a common cause of morbidity and mortality among immunocompromised patients. at the university of texas m. d. anderson cancer center (houston, tx), aspergillus terreus is second to a. fumigatus as the most common cause of ia. in the current study, the authors compared the risk factors and outcomes associated with ia caused by a. terreus and ia caused by a. fumigatus. | 2004 | 15378491 |
| [invasive infection by aspergillus terreus treated successfully with liposomal amphotericin b]. | a woman of 75 years old was admitted at our hospital for evaluation of worsening and weakness in inferior limbs. several vertebral fractures by crushing, one doubtful discitis, an infiltrate with cavitation in the right superior lobe and one infiltrate in the left superior lobe were detected. in the biopsy of the consolidation a filamentous fungus was watched and in the bronchial washing specimen culture grew aspergillus terreus. the infiltrates disappeared with liposomal amphotericin b remainin ... | 2004 | 15195481 |
| serotonin (5-ht) enhances the activity of amphotericin b against aspergillus fumigatus in vitro. | we investigated the in vitro synergistic antifungal potential of combining serotonin (5-ht) and sertraline with amphotericin b and itraconazole against clinical isolates of aspergillus spp. synergy tests were performed using the chequerboard microdilution method. activity was measured against aspergillus fumigatus (n = 7), aspergillus flavus (n = 3) and aspergillus terreus (n = 2), and compared with that for candida albicans and candida parapsilosis. the fractional inhibitory concentration (fic) ... | 2004 | 15380269 |
| antifungal therapy of murine aspergillus terreus infection. | aspergillus terreus is a species which is being seen increasingly frequently and which is highly resistant to amphotericin b in vitro and clinically. we evaluated amphotericin b, caspofungin, and posaconazole in a murine model of acute invasive aspergillosis. caspofungin and posaconazole both appeared beneficial and may be reasonable treatment alternatives for infection with a. terreus. | 2004 | 15388425 |
| rapid differentiation of aspergillus species from other medically important opportunistic molds and yeasts by pcr-enzyme immunoassay. | we developed a pcr-based assay to differentiate medically important species of aspergillus from one another and from other opportunistic molds and yeasts by employing universal, fungus-specific primers and dna probes in an enzyme immunoassay format (pcr-eia). oligonucleotide probes, directed to the internal transcribed spacer 2 region of ribosomal dna from aspergillus flavus, aspergillus fumigatus, aspergillus nidulans, aspergillus niger, aspergillus terreus, aspergillus ustus, and aspergillus v ... | 2004 | 15297489 |
| production of l-asparaginase by filamentous fungi. | l-asparaginase production was investigated in the filamentous fungi aspergillus tamarii and aspergillus terreus. the fungi were cultivated in medium containing different nitrogen sources. a. terreus showed the highest l-asparaginase (activity) production level (58 u/l) when cultivated in a 2% proline medium. both fungi presented the lowest level of l-asparaginase production in the presence of glutamine and urea as nitrogen sources. these results suggest that l-asparaginase production by of filam ... | 2004 | 15543411 |
| detection of aflatoxin-producing molds in korean fermented foods and grains by multiplex pcr. | an assay based on multiplex pcr was applied for the detection of potential aflatoxin-producing molds in korean fermented foods and grains. three genes, avfa, omta, and ver-1, coding for key enzymes in aflatoxin biosynthesis, were used as aflatoxin-detecting target genes in multiplex pcr. dna extracted from aspergillus flavus, aspergillus parasiticus, aspergillus oryzae, aspergillus niger, aspergillus terreus, penicillium expansum, and fusarium verticillioides was used as pcr template to test spe ... | 2004 | 15553652 |
| in vitro evaluation of double and triple combinations of antifungal drugs against aspergillus fumigatus and aspergillus terreus. | microdilution broth checkerboard techniques based on the national committee for clinical laboratory standards methodology were used to study double and triple antifungal combinations against clinical isolates of aspergillus fumigatus and a. terreus. the influences of the end-point definition (partial or complete inhibition) and the mode of reading (visually or spectrophotometrically) were determined. interactions between antifungal drugs were also evaluated by agar diffusion tests. combinations ... | 2004 | 14982791 |
| aspergillus endocarditis presenting as femoral artery embolism. | fungal valvular endocarditis is an unusual cause of endocarditis, yet very important because of its historically poor prognosis. we report two fatal cases of fungal valvular endocarditis following cardiovascular surgery, presenting as femoral artery embolism. aspergillus terreus and a. flavus were the causative agents of endocarditis in the two patients. diagnosis was established very early by culture of the emboli and was confirmed later by isolation of the same aspergillus species from the res ... | 2004 | 15189195 |
| [farmer's lung cases of a farmer and his son with high bal fluid beta-d glucan levels]. | a farmer and his son, who treated straw in a cowshed, were admitted to our hospital because of severe dyspnea during summer time. their chest x-ray films revealed bilateral reticulonodular shadows in the middle to lower lung fields. bronchoalveolar lavage (bal) fluid analyses showed a high proportion of lymphocytes and an increased cd4/8 ratio. they were diagnosed with farmer's lung and treated with pulse therapy with methylprednisolone and tapering of steroid. hypoxemia and interstitial shadow ... | 2004 | 15678910 |
| cardiac aspergillosis with pedunculated mass in the left ventricle. | immunosuppression can allow organisms that are not usually pathogenetic to cause disease; under such circumstances, aspergillus species frequently form large masses of fungal elements. we describe the case of a 12-year-old girl with hematologic remission of leukemia. she had a left ventricular pedunculated mass that was detected by echocardiographic study; at surgery, the presence of aspergillus terreus was confirmed. | 2004 | 15745301 |
| pellet morphology, culture rheology and lovastatin production in cultures of aspergillus terreus. | pellet growth of aspergillus terreus atcc 20542 in submerged batch fermentations in stirred bioreactors was used to examine the effects of agitation (impeller tip speed u(t) of 1.01-2.71 ms(-1)) and aeration regimens (air or an oxygen-enriched mixture containing 80% oxygen and 20% nitrogen by volume) on the fungal pellet morphology, broth rheology and lovastatin production. the agitation speed and aeration methods used did not affect the biomass production profiles, but significantly influenced ... | 2004 | 15652430 |
| fungal infection of sutureless self-sealing incision for cataract surgery. | to report the clinical picture and outcome of fungal infection of self-sealing wounds in cataract surgery. | 2003 | 14597526 |
| [biodegradation of herbicide pendimethalin by fungi and its characteristics]. | fungi, which can grow on and degrade pendimethalin, were isolated from activated sludge and soil. the biodegradation characterization in vitro was studied. the results showed that pendimethalin could be degraded by fungi, 16 fungi were isolated by enrichment culture, among which, 10 fungi could degrade more than 60% pendimethalin in 5 days in culture fluid. according to the physiological resistance and the capacity to degrade pendimethalin, three fungi were considered as efficiently degrading st ... | 2003 | 14997649 |
| epidemiology of aspergillus terreus at a university hospital. | invasive fungal infections due to aspergillus species have become a major cause of morbidity and mortality among immunocompromised patients. aspergillus terreus, a less common pathogen, appears to be an emerging cause of infection at our institution, the university of alabama hospital in birmingham. we therefore investigated the epidemiology of a. terreus over the past 6 years by using culture data; antifungal susceptibility testing with amphotericin b, voriconazole, and itraconazole; and molecu ... | 2003 | 14662934 |
| [a case of pulmonary aspergillosis caused by aspergillus terreus]. | 2003 | 15004970 | |
| detection and identification of fungi from fungus balls of the maxillary sinus by molecular techniques. | the aim of this study was to find a reliable method for the detection and identification of fungi in fungus balls of the maxillary sinus and to evaluate the spectrum of fungi in these samples. one hundred twelve samples were obtained from patients with histologically proven fungal infections; 81 samples were paraffin-embedded tissue sections of the maxillary sinus. in 31 cases, sinus contents without paraffin embedding were sent for investigation. pcr amplification with universal fungal primers ... | 2003 | 12574250 |
| effect of increasing inoculum sizes of aspergillus hyphae on mics and mfcs of antifungal agents by broth microdilution method. | in order to investigate the influence of different hyphal inoculum sizes on minimal inhibition concentrations (mics) and minimum fungicidal concentrations (mfcs) of amphotericin b (amb), voriconazole and itraconazole, five isolates each of aspergillus fumigatus, aspergillus flavus, aspergillus niger and aspergillus terreus were studied using a broth microdilution method. three inoculum sizes were used: 1 x 10(3)-5 x 10(3), 1 x 10(4)-5 x 10(4) and 1 x 10(5)-5 x 10(5) cfu/ml. mics and mfcs were re ... | 2003 | 12636983 |
| in vitro activities of three licensed antifungal agents against spanish clinical isolates of aspergillus spp. | the aim of the present study was to identify retrospectively trends in the species distributions and the susceptibility patterns of aspergillus species causing fungal infections in spanish medical centers from 2000 to 2002. the susceptibilities of 338 isolates to amphotericin b, itraconazole, and voriconazole were tested. aspergillus fumigatus was the most common species (54.7%), followed by aspergillus terreus (14.8%) and aspergillus flavus (13.9%). non-a. fumigatus species were encountered in ... | 2003 | 14506013 |
| microbial transformation of terreusinone, an ultraviolet-a (uv-a) protecting dipyrroloquinone, by streptomyces sp. | biotransformation study was conducted on the marine dipyrroloquinone, terreusinone (1) isolated from the marine-derived fungus aspergillus terreus. preparative-scale fermentation of terreusinone with streptomyces sp. has resulted in the isolation of a new oxidized metabolite, terreusinol (2). the structure was elucidated as 2-[(1r)-1-hydroxyisobutyl]-6-[(1r)-1,2-dihydroxyisobutyl]-1h,5h-pyrrolo[2,3-b]indole-4,8-dione (2) on the basis of physicochemical evidence. terreusinol (2) showed an ultravi ... | 2003 | 14646333 |
| disseminated aspergillus terreus infection arising from cutaneous inoculation treated with caspofungin. | a case of disseminated aspergillus terreus infection in a patient with prolonged neutropenia after stem cell transplant for myeloma is reported. the isolate was resistant to amphotericin b in vitro, and the patient was successfully managed with surgical debridement and the recently licensed antifungal agent caspofungin. there are many challenges associated with treating invasive aspergillosis, particularly that due to a. terreus, and the early use of caspofungin should be considered. | 2003 | 14686991 |
| cytotoxic constituents of aspergillus terreus from the rhizosphere of opuntia versicolor of the sonoran desert. | a novel cyclopentenedione, asterredione (1), two new terrecyclic acid a derivatives, (+)-5(6)-dihydro-6-methoxyterrecyclic acid a (2) and (+)-5(6)-dihydro-6-hydroxyterrecyclic acid a (3), and five known compounds, (+)-terrecyclic acid a (4), (-)-quadrone (5), betulinan a (6), asterriquinone d (7), and asterriquinone c-1 (8), were isolated from aspergillus terreus occurring in the rhizosphere of opuntia versicolor, using bioassay-guided fractionation. acid-catalyzed reaction of 2 under mild condi ... | 2003 | 14695798 |
| terreulactones a, b, c, and d: novel acetylcholinesterase inhibitors produced by aspergillus terreus. i. taxonomy, fermentation, isolation and biological activities. | in the course of screening for selective inhibitors of acetylcholinesterase from the microbial metabolites, four new meroterpenoid compounds, terreulactones a, b, c and d were isolated from solid state fermentation of aspergillus terreus fb000501. they showed potent inhibitory activities against acetylcholinesterase with ic50 values in range of 0.06 to approximately 0.42 microm. in addition, they exhibited more than 500 to approximately 3000 times selectivity for acetylcholinesterase compared wi ... | 2003 | 12817807 |
| terreulactones a, b, c, and d: novel acetylcholinesterase inhibitors produced by aspergillus terreus. ii. physico-chemical properties and structure determination. | terreulactones a, b, c, and d, new meroterpenoid compounds with potent anti-acetylcholinesterase activity, were determined to be mixed polyketides-terpenoid structures by spectroscopic studies. | 2003 | 12817808 |
| integrating transcriptional and metabolite profiles to direct the engineering of lovastatin-producing fungal strains. | we describe a method to decipher the complex inter-relationships between metabolite production trends and gene expression events, and show how information gleaned from such studies can be applied to yield improved production strains. genomic fragment microarrays were constructed for the aspergillus terreus genome, and transcriptional profiles were generated from strains engineered to produce varying amounts of the medically significant natural product lovastatin. metabolite detection methods wer ... | 2003 | 12536215 |
| acute myelogenous leukemia complicated by acute necrotizing ulcerative gingivitis due to aspergillus terreus. | infections caused by aspergillus terreus are rare but have been associated with a poor outcome in immunocompromised patients due to frequent resistance to conventional antifungal therapy. this report describes a case of a woman who developed acute necrotizing ulcerative gingivitis (anug) due to a. terreus during induction chemotherapy for acute myelogenous leukemia. she initially failed to respond to treatment with amphotericin b but the infection resolved following the introduction of oral itra ... | 2003 | 12769350 |
| modification of rapid susceptibility assay for antifungal susceptibility testing of aspergillus fumigatus. | to improve objectivity and speed of current antifungal mold susceptibility testing, the yeast rapid susceptibility assay (rsa) was adapted for aspergillus species. the rsa is based on glucose utilization in the presence of an antifungal drug. aspergillus fumigatus conidia were incubated in 0.2% glucose rpmi 1640 containing 0.03 to 16 micro g of amphotericin b or itraconazole/ml. drug-related inhibition of glucose utilization correlated with suppression of conidial germination. following incubati ... | 2003 | 12958253 |
| activity of micafungin (fk463) against an itraconazole-resistant strain of aspergillus fumigatus and a strain of aspergillus terreus demonstrating in vivo resistance to amphotericin b. | we compared the activity of four doses of micafungin (fk463) with that of amphotericin b, liposomal amphotericin b and itraconazole in a murine model of disseminated aspergillosis. temporarily neutropenic mice were infected with a lethal dose of either an itraconazole-resistant aspergillus fumigatus isolate or aspergillus terreus, a species that is less susceptible to amphotericin b. treatment was started 24 h after infection and lasted for 7 days. mice were treated with either amphotericin b (0 ... | 2003 | 12654746 |
| in vitro susceptibility of aspergillus spp. clinical isolates to albendazole. | the in vitro antifungal activity of albendazole, a benzimidazole widely used as an antihelmintic drug in humans, was investigated and assessed for its activity against aspergillus spp. forty-eight isolates, representing the most frequent species found in human pathology [aspergillus fumigatus (n = 27), aspergillus flavus (n = 10), aspergillus terreus (n = 7), aspergillus nidulans (n = 3) and aspergillus niger (n = 1)], and one quality control strain (a. niger atcc 9804 83435) were tested accordi ... | 2003 | 12716778 |
| peritonitis due to aspergillus and zygomycetes in patients undergoing peritoneal dialysis: report of 2 cases and review of the literature. | fungal peritonitis in patients on continuous ambulatory peritoneal dialysis (capd) has been associated with high mortality and high capd-discontinuation rates. most cases are due to candida spp. while aspergillus spp. and zygomycetes have only rarely been implicated. we report one case each of capd-related peritonitis caused by aspergillus terreus and mucor sp., which have previously been described in the literature once and twice, respectively. the former had a slowly progressive course, did no ... | 2003 | 12742319 |
| diagnosis of invasive septate mold infections. a correlation of microbiological culture and histologic or cytologic examination. | we correlated results of microbiologic culture and histopathologic examination for 2,891 consecutive samples from autopsy tissue, surgical or biopsy tissue, and bronchoalveolar lavage (bal) or bronchial washing (bw) specimens. for 23 autopsy cases with suspected invasive septate mold infections by histopathologic examination, culture yielded a mold in 12 cases (52%). for 1,683 surgical or biopsy samples, histopathologic evidence of invasive septate mold infection was present in 30 samples, 9 of ... | 2003 | 12817433 |
| transformations of cyclic nonaketides by aspergillus terreus mutants blocked for lovastatin biosynthesis at the lova and lovc genes. | two mutants of aspergillus terreus with either the lovc or lova genes disrupted were examined for their ability to transform nonaketides into lovastatin 1, a cholesterol-lowering drug. the lovc disruptant was able to efficiently convert dihydromonacolin l 5 or monacolin j 9 into 1, and could also transform desmethylmonacolin j 15 into compactin 3. in contrast, the lova mutant has an unexpectedly active beta-oxidation system and gives only small amounts of 1 upon addition of the immediate precurs ... | 2003 | 12929390 |