validation of bdccp2 as a marker for babesia divergens sexual stages in ticks.babesiosis is a tick-transmitted disease of mammalian hosts, caused by the intraerythrocytic protozoan parasites of the genus babesia. transmission of babesia parasites from the vertebrate host to the tick is mediated by sexual stages, the gametocytes which are the only intraerythrocytic stages that survive and develop inside the vector. very few data are available concerning these parasite stages and some markers are needed in order to refine our knowledge of babesia life cycle inside the tick ...201323103717
¹h, ¹⁵n and ¹³c backbone resonance assignments of a conformational mutant of the adhesion protein ∆-bd37 from babesia divergens.we report here the resonance assignment of edk-∆-bd37, conformational mutant potentially displaying the "open" conformation of bd37, a 25 kda surface protein from the apicomplexa parasite babesia divergens that could undergo drastic conformational changes during erythrocyte invasion.201322899250
antibody prevalence and molecular identification of babesia spp. in roe deer in a region-wide serologic study carried out in 2004 on free-ranging hunted roe deer in various landscapes, we found that 58% of the animals (237 out of 406) were antibody positive for babesia divergens antigen. serologic and infection status was also analyzed for 327 roe deer live-trapped in two fenced forest areas over 5 yr (2004-08). for two consecutive years during this period, 92 and 94% of the deer in these closed populations were antibody-positive for b. divergens. babesia spp. were isola ...201222493116
modelling bovine babesiosis: a tool to simulate scenarios for pathogen spread and to test control measures for the disease.tick-borne diseases are of increasing concern in many countries, particularly as a consequence of changes in land use and climate. ticks are vectors of numerous pathogens (viruses, bacteria, protozoa) that can be harmful to humans and animals. in the context of animal health, bovine babesiosis poses a recurrent threat to cattle herds. in this study, we use a modeling approach to investigate the spread of babesiosis and evaluate control measures. a previously developed tick population dynamics mo ...201222341037
evaluation of the inhibitory effect of n-acetyl-l-cysteine on babesia and theileria parasites.n-acetyl-l-cysteine is known to have antibacterial, antiviral, antimalarial, and antioxidant activities. therefore, the in vitro inhibitory effect of this hit was evaluated in the present study on the growth of babesia and theileria parasites. the in vitro growth of babesia bovis, babesia bigemina, babesia divergens, theileria equi, and babesia caballi that were tested was significantly inhibited (p < 0.05) by micromolar concentrations of n-acetyl-l-cysteine. the inhibitory effect of n-acetyl-l- ...201728655583
molecular identification and characterization of piroplasm species in hokkaido sika deer (cervus nippon yesoensis), japan.babesia and theileria species are tick-borne protozoan parasites that have a veterinary and zoonotic importance. in order to investigate the prevalence and genetic diversity of these parasites, a total of 269 sika deer blood dna samples collected from hokkaido, japan, were examined for babesia and theileria species by touch-down pcr targeting the 18s rrna gene. reverse line blot (rlb) hybridization was then used to detect 12 piroplasm species. the results revealed that 95.5% (257/269), 94.1% (25 ...201728651947
prevalence and distribution of borrelia and babesia species in ticks feeding on dogs in the u.k.ticks were collected during march-july 2015 from dogs by veterinarians throughout the u.k. and used to estimate current prevalences and distributions of pathogens. dna was extracted from 4750 ticks and subjected to polymerase chain reaction and sequence analysis to identify borrelia burgdorferi sensu lato (spirochaetales: spirochaetaceae) and babesia (piroplasmida: babesiidae) species. of 4737 ticks [predominantly ixodes ricinus linneaus (ixodida: ixodidae)], b. burgdorferi s.l. was detected in ...201728846148
high-resolution melting pcr assay, applicable for diagnostics and screening studies, allowing detection and differentiation of several babesia spp. infecting humans and animals.the goal of the study was to design a single tube pcr test for detection and differentiation of babesia species in dna samples obtained from diverse biological materials. a multiplex, single tube pcr test was designed for amplification of approximately 400 bp region of the babesia 18s rrna gene. universal primers were designed to match dna of multiple babesia spp. and to have low levels of similarity to dna sequences of other intracellular protozoa and babesia hosts. the pcr products amplified f ...201728795223
molecular identification and antigenic characterization of babesia divergens erythrocyte binding protein (bdebp) as a potential vaccine cell invasion is the only step where babesia parasites are extracellular, and their survival is menaced during this step. therefore, interfering with this critical stage is a target for an anti-babesia intervention strategy. in this regard, recombinant protein encoding babesia divergens erythrocyte binding protein (bdebp) was produced in escherichia coli in the current study, and its antiserum was prepared in mice for further molecular characterization. western blotting and indirect fluores ...201728743470
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