Publications

TitleAbstractYear
Filter
PMID
Filter
structural and serological characterization of the major glycolipid from rothia mucilaginosa.structural studies on the major glycolipid isolated from rothia mucilaginosa were carried out utilising specific chemical degradation, nmr spectroscopy and matrix-assisted laser-desorption/ionization time of flight mass spectrometry (maldi tof-ms). the glycolipid was found to be a dimannosylacylmonoglyceride in which the carbohydrate part was the glycerol-linked dimannoside alpha-d-manp-(1-->3)-alpha-d-manp-(1-->3)-sn-gro (man a-man b-gro), of which man b was esterified at o-6 by a fatty acid re ...200415535967
bacterial diversity in a nonsaline alkaline environment: heterotrophic aerobic populations.heterotrophic populations were isolated and characterized from an alkaline groundwater environment generated by active serpentinization, which results in a ca(oh)2-enriched, extremely diluted groundwater with ph 11.4. one hundred eighty-five strains were isolated in different media at different ph values during two sampling periods. to assess the degree of diversity present in the environment and to select representative strains for further characterization of the populations, we screened the is ...200415574939
evaluation of bacterial nitrate reduction in the human oral cavity.it is hypothesized that the enterosalivary nitrate circulation encourages nitrate reducing bacteria to reside within the oral cavity. nitrite production may then limit the growth of acidogenic bacteria as a result of the production of antimicrobial oxides of nitrogen, including nitric oxide. this study was carried out with 10 subjects to characterize oral nitrate reduction and identify the bacteria responsible. nitrate reduction varied between individuals (mean 85.4 +/- 15.9 nmol nitrite min(-1) ...200515693824
stomatococcus mucilaginosis infection leading to early cervical necrotizing fasciitis. 200515806065
severe acute tonsillitis caused by rothia dentocariosa in a healthy child.a 4-year-old japanese girl developed a sore throat and high fever. her tonsils were enlarged, red and covered with a thick white membrane. there was marked leukocytosis (26,600 leukocytes per mm) and elevated c-reactive protein levels (23.3 mg/dl). rothia dentocariosa was recovered from the throat swab; many gram-positive cocci were observed in the smear from the pseudomembrane covering the tonsil.200515876953
macrolide and lincosamide resistance in the gram-positive nasal and tonsillar flora of pigs.macrolide and lincosamide resistance phenotypes and the presence of the erm(a), erm(b), erm(c), and mef(a) genes were determined in 344 bacterial strains belonging to 34 species and nine genera, isolated from the tonsils and nasal cavities of 2-week- and 6-week-old piglets, derived from four different farms. these piglets had never before been treated with macrolides or lincosamides. macrolide and lincosamide resistance was most frequently present in streptococcus and enterococcus strains, of wh ...200312959408
bacteremia caused by rothia mucilaginosa in a patient with shwachman-diamond syndrome. 200717565469
efficacy of amikacin combinations for nocardiosis.we isolated five bacterial strains from patients diagnosed as having nocardiosis. bacterial species were identified based on the similarities in the nucleotide sequences of 16s ribosomal rnas. three of the five strains were identified as nocardia asteroids, but unexpectedly other two were streptomyces hygroscopicus and rothia dentocariosa. the latter two species are not members of the family nocardiaceae. we investigated the susceptibilities of these five strains to the following nine antimicrob ...200314649737
bacterial meningitis from rothia mucilaginosa in patients with malignancy or undergoing hematopoietic stem cell transplantation.opportunistic infections contribute to morbidity and mortality of patients undergoing hematopoietic stem cell transplantation and treatment for malignancies. rothia mucilaginosa, a gram-positive bacterium, is responsible for rare, but often fatal meningitis in severely immunocompromised patients. we describe two cases of meningitis from discrete strains of r. mucilaginosa on our pediatric bone marrow transplant unit, summarize the published cases of r. mucilaginosa meningitis in oncology and ste ...200817588235
stomatococcus mucilaginosus meningitis in a patient with acute lymphoblastic leukemia. 199517590615
stability of microbial communities in goat milk during a lactation year: molecular approaches.the microbial communities in milks from one herd were evaluated during 1-year of lactation, using molecular methods to evaluate their stability and the effect of breeding conditions on their composition. the diversity of microbial communities was measured using two approaches: molecular identification by 16s and 18s rdna sequencing of isolates from counting media (two milks), and direct identification using 16s rdna from clone libraries (six milks). the stability of these communities was evaluat ...200717604934
eight cases of lower respiratory tract infection caused by stomatococcus mucilaginosus.stomatococcus mucilaginosus was isolated from 8 patients suffering from lower respiratory tract infections over a 4-y period (1999-2003). the infections ranged from mild cases of pneumonia to a life-threatening case of recurrent lung abscesses in a neutropenic patient. the various strains were cultured from specimens obtained by bronchoscopy, blood, and sputum specimens. all strains were fully susceptible to rifampicin, vancomycin and gentamicin. they showed variable susceptibility to penicillin ...200717886126
intrauterine fetal death associated with rothia dentocariosa: a case report.we report a case of intrauterine fetal death at term with a possible association to an oral bacterium, rothia dentocariosa. a healthy primiparous woman was admitted at term with an intrauterine fetal death after an uncomplicated pregnancy. r. dentocariosa was found in the fetal blood culture.200717980173
recurrent chronic ambulatory peritoneal dialysis-associated infection due to rothia dentocariosa.rothia dentocariosa is a commensal organism of the human oropharynx. clinical infection due to this organism is rare. a case of recurrent peritoneal dialysis-related peritonitis caused by r dentocariosa and a review of the literature is reported. isolation of r dentocariosa from dialysate fluid should not be dismissed as a contaminant. although there are no interpretive criteria for antimicrobial susceptibility testing, r dentocariosa appears to be susceptible to a variety of antibiotics includi ...200418159489
inhibition of microbial adhesion to silicone rubber treated with biosurfactant from streptococcus thermophilus a.microbial adhesion of four bacterial and two yeast strains isolated from explanted voice prostheses to silicone rubber before and after conditioning with a biosurfactant obtained from the probiotic bacterium streptococcus thermophilus a was investigated in a parallel plate flow chamber. the silicone rubber with and without an adsorbed biosurfactant layer was characterized using contact angle measurements. water contact angles indicated that the silicone rubber surface with adsorbed biosurfactant ...200616420603
rothia terrae sp. nov. isolated from soil in taiwan.a cream-white-coloured, aerobic, gram-positive, ovoid to spherical-shaped bacterial strain, designated l-143(t), was isolated from soil in taiwan. the highest 16s rrna gene sequence similarities of strain l-143(t) (98.3-95.8%) were with members of the genus rothia. chemotaxonomic and phenotypic properties of this organism were consistent with its classification in the genus rothia. the novel isolate was distinguished from all rothia species by several phenotypic characteristics. the peptidoglyca ...200818175688
[rothia mucilaginosa]. 200818273521
in vitro activity of daptomycin against clinical isolates of gram-positive bacteria.we determined the activity of daptomycin, a recently fda-approved antimicrobial agent, against clinical isolates of gram-positive bacteria, including viridans group streptococci (16 streptococcus mitis species group, 12 s. mutans species group, 9 s. anginosus species group, 8 s. sanguinis species group, 5 s. salivarius species group) from patients with infective endocarditis, 32 methicillin-resistant staphylococcus aureus, 32 high-level penicillin-resistant streptococcus pneumoniae, 38 vancomyci ...200516133715
rothia aeria as a cause of sepsis in a native joint.rothia aeria is a recently described gram-positive rod from the family micrococcaceae. an elderly woman with rheumatoid arthritis and dental abscesses who was undergoing immunosuppression had r. aeria isolated from synovial fluid. this report characterizes this rare organism and contributes to the literature on its pathogenicity and likely oral source.201020504983
stomatococcus mucilaginosus meningitis in a healthy 2-month-old child.stomatococcus mucilaginosus is a gram-positive, catalase-variable organism considered part of the normal human oral and upper respiratory tract flora. although traditionally believed to be an organism of low virulence, stomatococcus mucilaginosus has been reported to be an opportunistic pathogen in immunocompromised patients. we describe what we believe is the first reported case of stomatococcus mucilaginosus meningitis in a healthy child. the isolate was multidrug-resistant, susceptible only t ...200818287304
mlpa diagnostics of complex microbial communities: relative quantification of bacterial species in oral biofilms.a multitude of molecular methods are currently used for identification and characterization of oral biofilms or for community profiling. however, multiplex pcr techniques that are able to routinely identify several species in a single assay are not available. multiplex ligation-dependent probe amplification (mlpa) identifies up to 45 unique fragments in a single tube pcr. here we report a novel use of mlpa in the relative quantification of targeted microorganisms in a community of oral microbiot ...200818824042
microbiological profiles in saliva and supragingival plaque from caries-active adolescents before and after a short-term daily intake of milk supplemented with probiotic bacteria - a pilot study.the aim of the present pilot study was to investigate the microbial profile in saliva and supragingival plaque samples collected from caries-active adolescents before and after a daily short-term intake of milk supplemented with the probiotic bacteria.201021180676
study of microbial diversity in raw milk and fresh curd used for fontina cheese production by culture-independent methods.the bacterial populations of raw milk employed for the production of fontina cheese in alpine farms located in different valleys and altitudes (from 700 to 2246 m above sea level) were investigated by culture independent techniques. total microbial dna was isolated from milk and curd samples and used as template in polymerase chain reaction (pcr) to study the hypervariable v3 region of the bacterial 16s rrna gene and analyzed by denaturing gradient gel electrophoresis (dgge). representative band ...200919232767
bacterial diversity in the human saliva from different ages.to obtain primary idea on oral bacterium species that are generally present in periodotally healthy koreans, the oral bacterial flora in the saliva of four periodontally healthy koreans at different ages (5, 32, 35, 65) was investigated in this study. for this investigation, 16srrna gene clone libraries were generated from the saliva of the four healthy koreans, and 50 clones were randomly selected from each saliva clone library and sequenced. totally, 37 different kinds of bacterial 16s rrna ge ...200617082753
first case report of sepsis due to rothia aeria in a neonate.rothia aeria, a gram-positive coccoid- to rod-shaped bacterium with irregular morphology, is an extremely rare causative organism of infections in humans. we report the first case of r. aeria sepsis clinically manifested in a female neonate soon after birth.200919261789
[oral cavity microbiocenose in healthy teenagers and patients with chronic gastritis and gastroduodenitis].microbial association distinctive features and colonization degree of different oral cavity biotope were established in healthy teenagers and patients with chronic gastritis and gastroduodenitis. there were studied mucous membrane surfaces of cheek, tongue, content of gingival crevice, oral fluid and dental plaque of 24 healthy persons of the age 12-17 years and 29 patients of the same age with gastritis and gastroduodenitis. on the mucous membranes of the ill teenagers there were found porphiro ...200919491778
rothia dentocariosa isolated from a corneal ulcer.rothia dentocariosa is a common commensal in the oropharyngeal cavity but a rare human pathogen. ocular culture has been documented only twice previously, both from vitreous samples taken in the context of endophthalmitis. these cases, and other reports of human rothia infection, have proposed hematogenous spread from the oropharynx as the mode of transmission.200617133072
caries-related plaque microcosm biofilms developed in microplates.in vivo dental plaque biofilms consist of complex communities of oral bacteria that are a challenge to replicate in vitro. the aim of this investigation was to establish human dental plaque microcosms in microplates to reflect conditions that are relevant to dental caries. microcosm plaque biofilms were initiated from the saliva of two different donors, grown for up to 10 days in 24-welled microplates on thermanox coverslips in various types of artificial saliva with and without sucrose, which w ...200717311629
determination of the genetic support for tet(w) in oral bacteria.the dna sequence flanking a tet(w) gene in an oral rothia sp. was determined. the gene was linked to two different transposases, and these were flanked by two almost identical mef (macrolide efflux) genes. this structure was found in 4 out of 20 tet(w)-containing oral bacteria investigated.200717371816
rothia dentocariosa bacteremia in children: report of two cases and review of the literature.rothia dentocariosa, a pleomorphic, fastidious, gram-positive rod, is a normal inhabitant of the oropharynx. it is a well-known causative agent of dental plaques and periodontal disease. generally regarded as of low virulence to humans, r. dentocariosa has been increasingly recognized as a pathogen in adults and often associated with infective endocarditis. it should not necessarily be regarded as a contaminant when the isolate comes from areas other than the oropharynx, especially from the bloo ...200717493907
proximal small intestinal microbiota and identification of rod-shaped bacteria associated with childhood celiac disease.alterations in the composition of the microbiota in the intestine may promote development of celiac disease (cd). using scanning electron microscopy (sem) we previously demonstrated that rod-shaped bacteria were present on the epithelium of proximal small intestine in children with cd but not in controls. in this study we characterize the microbiota of proximal small intestine in children with cd and controls and identify cd-associated rod-shaped bacteria.200919755974
infectious granulomatous dermatitis associated with rothia mucilaginosa bacteremia: a case report.infections with rare pathogens are being recognized with increasing frequency in severely immunocompromised patients. as a result of these patients' underlying compromised defenses and susceptibility to atypical organisms, tissue biopsies from patients within this population may demonstrate nonclassical histopathological findings. here, we describe an unusual granulomatous reaction to gram-positive cocci in the skin of a 52-year-old man undergoing salvage chemotherapy for acute myeloid leukemia. ...201019940746
septic arthritis caused by rothia dentocariosa in a patient with rheumatoid arthritis receiving etanercept therapy. 200919966198
defining the healthy "core microbiome" of oral microbial communities.most studies examining the commensal human oral microbiome are focused on disease or are limited in methodology. in order to diagnose and treat diseases at an early and reversible stage an in-depth definition of health is indispensible. the aim of this study therefore was to define the healthy oral microbiome using recent advances in sequencing technology (454 pyrosequencing).200920003481
late prosthetic joint infection due to rothia mucilaginosa.we report a chronic hip arthroplasty infection with rothia mucilaginosa, a gram-positive germ belonging to the normal flora of the human oral cavity. successful treatment was achieved by a two-stage hip arthroplasty revision and intravenous administration of vancomycin. this case report illustrates the potential virulence of r. mucilaginosa in patients with a joint prosthesis. we propose to routinely perform specific staining and prolonged culturing techniques for unusual germs such as rothia mu ...200717515244
relationship between oral malodor and the global composition of indigenous bacterial populations in saliva.oral malodor develops mostly from the metabolic activities of indigenous bacterial populations within the oral cavity, but whether healthy or oral malodor-related patterns of the global bacterial composition exist remains unclear. in this study, the bacterial compositions in the saliva of 240 subjects complaining of oral malodor were divided into groups based on terminal-restriction fragment length polymorphism (t-rflp) profiles using hierarchical cluster analysis, and the patterns of the microb ...201020228112
prevalence and diversity of microbes in the amniotic fluid, the fetal inflammatory response, and pregnancy outcome in women with preterm pre-labor rupture of membranes.the role played by microbial invasion of the amniotic cavity (miac) in preterm pre-labor rupture of membranes (pprom) is inadequately characterized, in part because of reliance on cultivation-based methods.201020331587
rothia aeria acute bronchitis: the first reported case. 201020358247
[first case report of respiratory infection with rothia aeria].a 53-year-old woman was admitted to our hospital with dysbasia and forgetfulness. her past history included uveitis at age 39. medical examinations led to a diagnosis of neurosarcoidosis. although she was treated with prednisolone, her symptoms remained, so she received steroid pulse therapy twice, and administration of azathioprine. in early january 2007, a chest x-ray film showed nodules in the right upper lung that rapidly increased in size and number. a ct scan revealed multiple nodules incl ...201020387527
a case of peritonitis due to rothia mucilaginosa. 201020424204
bacteria that masquerade as fungi: actinomycosis/nocardia.the order actinomycetales includes phylogenetically diverse but morphologically similar aerobic and anaerobic bacteria that exhibit filamentous branching structures which fragment into bacillary or coccoid forms. the aerobic actinomyces are a large, diverse group of gram-positive bacteria including nocardia, gordona, tsukamurella, streptomyces, rhodococcus, streptomycetes, mycobacteria, and corynebacteria. the anaerobic genera of medical importance include actinomyces, arachnia, rothia, and bifi ...201020463251
microbial diversity of supra- and subgingival biofilms on freshly colonized titanium implant abutments in the human mouth.supra- and subgingival biofilm formation is considered to be mainly responsible for early implant failure caused by inflammations of periimplant tissues. nevertheless, little is known about the complex microbial diversity and interindividual similarities around dental implants. an atraumatic assessment was made of the diversity of microbial communities around titanium implants by single strand conformation polymorphism (sscp) analysis of the 16s rrna gene amplicons as well as subsequent sequence ...201020931254
16s rrna gene-based identification of cultured bacterial flora from host-seeking ixodes ricinus, dermacentor reticulatus and haemaphysalis concinna ticks, vectors of vertebrate pathogens.a total of 151 bacterial isolates were recovered from different developmental stages (larvae, nymphs and adults) of field-collected ticks (67 strains from ixodes ricinus, 38 from dermacentor reticulatus, 46 from haemaphysalis concinna). microorganisms were identified by means of 16s rrna gene sequencing. almost 87 % of the strains belonged to g(+) bacteria with predominantly occurring genera bacillus and paenibacillus. other g(+) strains included arthrobacter, corynebacterium, frigoribacterium, ...200919937215
review of airway illnesses by kytococcus and rothia and a look at inhalatory vancomycin as a treatment support.seriously compromised patients may acquire deep airway ailments by gram positive, commensal bacteria including kytococcus and rothia, pathogenic tracts of which still remain quite unexplored. resistances they express have been poorly investigated over the years, and no published guidelines for susceptibility testing and antibiotic therapy exist. we would therefore revise the current knowledge about these opportunistic organisms' clinical impact, as well as discuss on recent patents focusing on l ...201121210763
microbial communities of the upper respiratory tract and otitis media in children.streptococcus pneumoniae asymptomatically colonizes the upper respiratory tract of children and is a frequent cause of otitis media. patterns of microbial colonization likely influence s. pneumoniae colonization and otitis media susceptibility. this study compared microbial communities in children with and without otitis media. nasal swabs and clinical and demographic data were collected in a cross-sectional study of philadelphia, pa, children (6 to 78 months) (n=108) during the 2008-2009 winter ...201121285435
rothia dentocariosa endocarditis with mitral valve prolapse: case report and brief review. 201121311946
alkaloids of rothia trifoliata and rothia hirsuta. 198821401151
culturable bacterial diversity at the princess elisabeth station (utsteinen, sør rondane mountains, east antarctica) harbours many new taxa.we studied the culturable heterotrophic bacterial diversity present at the site of the new princess elisabeth station at utsteinen (dronning maud land, east antarctica) before construction. about 800 isolates were picked from two terrestrial microbial mat samples after incubation on several growth media at different temperatures. they were grouped using rep-pcr fingerprinting and partial 16s rrna gene sequencing. phylogenetic analysis of the complete 16s rrna gene sequences of 93 representatives ...201121501941
using ruditapes philippinarum conglutination mud to produce bioflocculant and its applications in wastewater treatment.a novel bioflocculant-producing bacterium, zht4-13, was isolated from ruditapes philippinarum conglutination mud. by biomicroscope morphological observation, 16s rdna sequence identification and physiological and biochemical characteristics, strain zht4-13 was identified as rothia sp. the bioflocculant mbf4-13 produced by strain zht4-13 had a flocculating efficiency of 86.22% for 5 g l(-1) kaolin clay suspension when the initial ph was 9 and the temperature was 20 degrees c. it had flocculating ...200919535246
[paucisymptomatic pneumonia due to rothia mucilaginosa: case report and literature review].rothia mucilaginosa is a gram-positive, coagulase-negative, encapsulated, non-spore-forming coccus considered part of the commensal flora of the oral cavity and the upper respiratory tract in humans. its involvement has been reported in an increasing spectrum of infections, above all among immunocompromised patients. to date, only 11 cases of pneumonia due to rothia mucilaginosa have been described in the literature. the authors report a case of pneumonia due to rothia mucilaginosa in a 72-year- ...200919602923
comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray.this study compared the subgingival microbiota of subjects with refractory periodontitis (rp) to those in subjects with treatable periodontitis (grs = good responders) or periodontal health (ph) using the human oral microbe identification microarray (homim).200919722792
detection of nine microorganisms from the initial carious root lesions using a taqman-based real-time pcr.oral diseases (2011) objective:  the purpose of this study was to quantify nine selected cariogenic bacteria in plaque from sound root surfaces and initial carious root lesions using taqman pcr and to analyse a putative dependence on the kind of initial periodontal treatment. material and methods:  fifty-four subjects with generalized chronic periodontitis were randomly allocated to one of the following initial periodontal therapies: full-mouth disinfection, full-mouth scaling and root planing o ...201121605286
identification of airborne bacterial and fungal species in the clinical microbiology laboratory of a university teaching hospital employing ribosomal dna (rdna) pcr and gene sequencing techniques.universal or "broad-range" pcr-based ribosomal dna (rdna) was performed on a collection of 58 isolates (n = 30 bacteria + 28 fungi), originating from environmental air from several locations within a busy clinical microbiology laboratory, supporting a university teaching hospital. a total of 10 bacterial genera were identified including both gram-positive and gram-negative genera. gram-positive organisms accounted for 27/30 (90%) of total bacterial species, consisting of seven genera and include ...200920183192
septic arthritis due to rothia mucilaginosa. 201021153041
[studies on the actinobacterial diversity in aiding lake sediments].this study aims at investigating the diversity of actinobacteria in aiding lake, a hypersaline lake and the lowest land point in china.201122097767
rothia dentocariosa sepsis in a pediatric renal transplant recipient having post-transplant lymphoproliferative disorders.rothia dentocariosa (rd) is a gram-positive rod that colonizes the human oral cavity and can cause infective endocarditis.200616677365
tuberculosis-like pneumonias by the aerobic actinomycetes rhodococcus, tsukamurella and gordonia.the order actinomycetales includes phylogenetically diverse but morphologically similar aerobic and anaerobic organisms, exhibiting filamentous branching structures which fragment into rods or coccoid forms. lung pathogens of the order comprise mycobacterium, nocardia, corynebacterium, actinomyces, kytococcus, rothia, williamsia, as well as gordonia, tsukamurella and rhodococcus. particularly, members of the last three genera are uncommon aerobic agents of lung cavitations and tuberculosis(tb)-l ...201122192786
identification of rothia bacteria as gluten-degrading natural colonizers of the upper gastro-intestinal tract.gluten proteins, prominent constituents of barley, wheat and rye, cause celiac disease in genetically predisposed subjects. gluten is notoriously difficult to digest by mammalian proteolytic enzymes and the protease-resistant domains contain multiple immunogenic epitopes. the aim of this study was to identify novel sources of gluten-digesting microbial enzymes from the upper gastro-intestinal tract with the potential to neutralize gluten epitopes.201121957450
[pneumonia and rothia dentocariosa]. 201121908119
Comparative analysis of salivary bacterial microbiome diversity in edentulous infants and their mothers or primary care givers using pyrosequencing.Bacterial contribution to oral disease has been studied in young children, but there is a lack of data addressing the developmental perspective in edentulous infants. Our primary objectives were to use pyrosequencing to phylogenetically characterize the salivary bacterial microbiome of edentulous infants and to make comparisons against their mothers. Saliva samples were collected from 5 edentulous infants (mean age?=?4.6±1.2 mo old) and their mothers or primary care givers (mean age?=?30.8±9.5 y ...201121853142
phylogenetic perspectives of nitrogen-fixing actinobacteria.it was assumed for a long time that the ability to catalyze atmospheric nitrogen (diazotrophy) has a narrow distribution among actinobacteria being limited to the genus frankia. recently, the number of nitrogen fixation (nifh) genes identified in other non-frankia actinobacteria has dramatically increased and has opened investigation on the origin and emergence of diazotrophy among actinobacteria. during the last decade, mycobacterium flavum, corynebacterium autotrophicum and a fluorescent arthr ...201121779790
Displaying items 201 - 261 of 261