| degradation of chlorobenzenes at nanomolar concentrations by burkholderia sp. strain ps14 in liquid cultures and in soil. | the utilization of 1,2,4,5-tetrachloro-, 1,2,4-trichloro-, the three isomeric dichlorobenzenes and fructose as the sole carbon and energy sources at nanomolar concentrations was studied in batch experiments with burkholderia sp. strain ps14. in liquid culture, all chlorobenzenes were metabolized within 1 h from their initial concentration of 500 nm to below their detection limits of 0.5 nm for 1,2,4,5-tetrachloro- and 1,2,4-trichlorobenzene and 7.5 nm for the three dichlorobenzene isomers, with ... | 1999 | 10347041 |
| in vitro activities of designed antimicrobial peptides against multidrug-resistant cystic fibrosis pathogens. | the emergence of multidrug-resistant pathogens renders antibiotics ineffective in the treatment of lung infections in patients with cystic fibrosis (cf). designed antimicrobial peptides (daps) are laboratory-synthesized peptide antibiotics that demonstrate a wide spectrum of antibacterial activity. optimal conditions for susceptibility testing of these peptides have not yet been established. medium composition is clearly a major factor influencing the results and reproducibilities of susceptibil ... | 1999 | 10348766 |
| [the role of the structural parts of bacterial lipopolysaccharide in its direct immunosuppressive activity]. | the direct immunosuppressive activity of lipopolysaccharides (lps) and their structural parts (o-chains, r-core, lipid a), obtained from salmonella, pseudomonas and burkholderia, was studied. lps preparations were extracted by the phenol-water method. structural parts of lps were obtained by acetic acid hydrolysis and gel filtration. the study demonstrated that all these preparations, when injected intraperitoneally into mice, did not affect the level of delayed-type hypersensitivity (dth) to th ... | 1999 | 10356729 |
| [the efficacy and outlook for the study of live vaccines for the prevention of melioidosis]. | the effect of immunization with burkholderia pseudomallei, (pur- and ts), heterologous vaccines and the recombinant culture of francisella tularensis rm2, carrying a plasmid with fragments of b. pseudomallei chromosome, was studied on four species of experimental animals, essentially differing by their sensitivity to melioidosis. b. pseudomallei mutants formed the statistically significant level of protection in subcutaneously challenged animals, moderately sensitive to melioidosis, but were not ... | 1999 | 10356737 |
| a novel mucin-sulphatase activity found in burkholderia cepacia and pseudomonas aeruginosa. | lung infections due to burkholderia cepacia and pseudomonas aeruginosa in patients with cystic fibrosis (cf) are common, are associated with respiratory morbidity and are a cause of mortality. respiratory mucin in cf patients is highly sulphated, which increases its resistance to bacterial degradation. desulphation increases the susceptibility of mucin to degradation by bacterial glycosidases and proteinases, and subsequent deglycosylation may facilitate bacterial colonisation by increasing avai ... | 1999 | 10359304 |
| identification of burkholderia spp. in the clinical microbiology laboratory: comparison of conventional and molecular methods. | cystic fibrosis (cf) predisposes patients to bacterial colonization and infection of the lower airways. several species belonging to the genus burkholderia are potential cf-related pathogens, but microbiological identification may be complicated. this situation is not in the least due to the poorly defined taxonomic status of these bacteria, and further validation of the available diagnostic assays is required. a total of 114 geographically diverse bacterial isolates, previously identified in re ... | 1999 | 10364579 |
| differentiation of burkholderia species by pcr-restriction fragment length polymorphism analysis of the 16s rrna gene and application to cystic fibrosis isolates. | burkholderia cepacia, which is an important pathogen in cystic fibrosis (cf) owing to the potential severity of the infections and the high transmissibility of some clones, has been recently shown to be a complex of five genomic groups, i.e., genomovars i, ii (b. multivorans), iii, and iv and b. vietnamiensis. b. gladioli is also involved, though rarely, in cf. since standard laboratory procedures fail to provide an accurate identification of these organisms, we assessed the ability of restricti ... | 1999 | 10364586 |
| lung infections in pediatric lung transplantation: experience in 49 cases. | pulmonary infections, and particularly cytomegalovirus (cmv) infections, are a major cause of morbidity after lung transplantation. we report here our results in 49 pediatric lung transplantations. | 1999 | 10371127 |
| an indigenous melioidosis: a case report. | melioidosis is a rare but potentially fatal infectious disease in taiwan, although it has been endemic in southeast asia, especially northeast thailand, and northern australia. in this article, we report a male diabetes with fulminant pneumonia, and septicemia caused by burkholderia pseudomallei without traveling abroad before this episode. productive cough and intermittent chills, high fever for one week, followed by progressively deteriorating dyspnea, shock, disturbed consciousness status wer ... | 1999 | 10375872 |
| obligatory role of gamma interferon for host survival in a murine model of infection with burkholderia pseudomallei. | burkholderia pseudomallei, the causative agent of melioidosis, is a gram-negative bacterium capable of causing either acute lethal sepsis or chronic but eventually fatal disease in infected individuals. however, despite the clinical importance of this infection in areas where it is endemic, there is essentially no information on the mechanisms of protective immunity to the bacterium. we describe here a murine model of either acute or chronic infection with b. pseudomallei in taylor outbred (to) ... | 1999 | 10377144 |
| characterization of metal-resistant soil eubacteria by polymerase chain reaction--denaturing gradient gel electrophoresis with isolation of resistant strains. | contamination of soils with heavy metal ions is a major problem on industrial and defense-related sites worldwide. the bioavailability and mobility of these contaminants is partially determined by the microbial biomass present at these sites. in this study, we have assessed the effect of the addition of a mixture of toxic metal salts on the prokaryotic community of microcosms consisting of sandy-loam soil using direct molecular analysis of the recoverable eubacterial 16s rdna molecules by polyme ... | 1999 | 10380644 |
| cis-2,3-dihydro-2,3-dihydroxybiphenyl dehydrogenase and cis-1, 2-dihydro-1,2-dihydroxynaphathalene dehydrogenase catalyze dehydrogenation of the same range of substrates. | pseudomonas putida strain g7 cis-1,2-dihydro-1, 2-dihydroxynaphthalene dehydrogenase (nahb) and comamonas testosteroni strain b-356 cis-2,3-dihydro-2,3-dihydroxybiphenyl dehydrogenase (bphb) were found to be catalytically active towards cis-2,3-dihydro-2,3-dihydroxybiphenyl (specificity factors of 501 and 5850 s-1 mm-1 respectively), cis-1,2-dihydro-1, 2-dihydroxynaphthalene (specificity factors of 204 and 193 s-1 mm-1 respectively) and 3,4-dihydro-3,4-dihydroxy-2,2',5, 5'-tetrachlorobiphenyl (s ... | 1999 | 10381363 |
| identification of a putative p-transporter operon in the genome of a burkholderia strain living inside the arbuscular mycorrhizal fungus gigaspora margarita. | this article reports the identification of a putative p-transporter operon in the genome of a burkholderia sp. living in the cytoplasm of the arbuscular mycorrhizal fungus gigaspora margarita. its presence suggests that burkholderia sp. has the potential for p uptake from this environment. this finding raises new questions concerning the importance of intracellular bacteria for mycorrhizal symbiosis. | 1999 | 10383982 |
| biochemical-genetic analysis and distribution of far-1, a class a beta-lactamase from nocardia farcinica. | from genomic dna of the clinical isolate nocardia farcinica vic, a 1. 6-kb sau3ai fragment was cloned and expressed in escherichia coli jm109. the recombinant strain expressed a beta-lactamase (pi, 4.6), far-1, which conferred high levels of resistance to amoxicillin, piperacillin, ticarcillin, and cephalothin. the hydrolysis constants (kcat, km, ki, and 50% inhibitory concentration) confirmed the mic results and showed that far-1 activity is inhibited by clavulanic acid and at a low level by ta ... | 1999 | 10390216 |
| nosocomial acquisition of burkholderia gladioli in patients with cystic fibrosis. | | 1999 | 10390428 |
| amplified rdna restriction analysis and further genotypic characterisation of metal-resistant soil bacteria and related facultative hydrogenotrophs. | the level of genotypic relationship between czc+ soil bacteria mainly resistant to zinc (but also to various other metals), and related facultative hydrogenotrophs previously assigned to the genera alcaligenes, ralstonia, and burkholderia was evaluated using ardra (amplified ribosomal dna restriction analysis). the analysis included 44 strains isolated from harsh industrial environments in sediments, soils and wastes with high content of heavy metals. these strains were selected by their ability ... | 1999 | 10390877 |
| short-chain acyl-coa-dependent production of oxalate from oxaloacetate by burkholderia glumae, a plant pathogen which causes grain rot and seedling rot of rice via the oxalate production. | in burkholderia glumae (formerly named pseudomonas glumae), isolated as the causal agent of grain rot and seedling rot of rice, oxalate was produced from oxaloacetate in the presence of short-chain acyl-coa such as acetyl-coa and propionyl-coa. upon purification, the enzyme responsible was separated into two fractions (tentatively named fractions ii and iii), both of which were required for the acyl-coa-dependent production of oxalate. in conjugation with the oxalate production from oxaloacetate ... | 1999 | 10393345 |
| specificity of the lipase-specific foldases of gram-negative bacteria and the role of the membrane anchor. | folding of lipases that are secreted by pseudomonads and other gram-negative bacteria via the type ii secretion pathway is facilitated by dedicated chaperones, called lipase-specific foldases (lifs). lifs are membrane-anchored proteins with a large periplasmic domain. the functional interaction between the lif and its cognate lipase is specific, since the pseudomonas aeruginosa lif was found not to substitute for lifs from burkholderia glumae or acinetobacter calcoaceticus. however, the p. aerug ... | 1999 | 10394914 |
| tuning biphenyl dioxygenase for extended substrate specificity. | highly substituted polychlorinated biphenyls (pcbs) are known to be very resistant to aerobic biodegradation, particularly the initial attack by biphenyl dioxygenase. functional evolution of the substrate specificity of biphenyl dioxygenase was demonstrated by dna shuffling and staggered extension process (step) of the bpha gene coding for the large subunit of biphenyl dioxygenase. several variants with an extended substrate range for pcbs were selected. in contrast to the parental biphenyl diox ... | 1999 | 10397810 |
| evidence for the presence in burkholderia pseudomallei of a type iii secretion system-associated gene cluster. | burkholderia pseudomallei, the causative agent of melioidosis, contains a cluster of putative genes homologous to those encoding hpap, hrcq, hrcr, hrcs and hrpv in the plant pathogen ralstonia solanacearum. in r. solanacearum, these genes form part of a type iii secretion-associated pathogenicity island. the order of the genes in b. pseudomallei is directly equivalent to that found in r. solanacearum. the b. pseudomallei proteins share 49.5% (hpap), 52.6% (hrcq), 80.0% (hrcr), 72.1% (hrcs) and 4 ... | 1999 | 10403415 |
| analysis of flic variation among clinical isolates of burkholderia cepacia. | pcr and restriction fragment length polymorphism (rflp) typing of flagellin genes (flic) from 57 clinical isolates of burkholderia cepacia indicated that only type 11 flagellins were present. twenty-two isolates previously identified as the epidemic uk cystic fibrosis strain were indistinguishable by this method, as were 11 isolates from a pseudo-outbreak in senegal. other clinical isolates, including 19 from disparate sources in malaysia, were separated into nine flic rflp groups, exhibiting a ... | 1999 | 10403416 |
| biosynthesis and characterization of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by burkholderia cepacia d1. | copolyesters of 3-hydroxybutyrate (3hb) and 3-hydroxyvalerate (3hv) were produced by burkholderia cepacia d1 at 30 degrees c in nitrogen-free culture solutions containing n-butyric acid and/or n-valeric acid. when n-valeric acid was used as the sole carbon source, the 3hv fraction in copolyester increased from 36 to 90 mol% as the concentration of n-valeric acid in the culture solution increased from 1 to 20 g/l. the addition of n-butyric acid to the culture solution resulted in a decrease in th ... | 1999 | 10408637 |
| surgical presentation of melioidosis in india. | melioidosis, the disease caused by burkholderia pseudomallei, is common in southeast asia. it has also been reported from india, where some investigators feel it is under-diagnosed and under-reported. we report our experience with melioidosis presenting as abscesses at unusual sites. | 1999 | 10416320 |
| a murine model for infection with burkholderia cepacia with sustained persistence in the spleen. | burkholderia cepacia is an opportunistic pathogen that causes severe systemic infections in patients with chronic granulomatous disease (cgd) or with cystic fibrosis (cf), but its mechanisms of virulence are poorly understood. we developed a murine model of systemic infection in wild-type (wt) and gamma interferon knockout (gko) balb/c mice to facilitate dissection of components of pathogenicity and host defense. both wt and gko mice were susceptible to chronic splenic infection with b. cepacia, ... | 1999 | 10417170 |
| molecular characterization of genetic loci required for secretion of exoproducts in burkholderia pseudomallei. | previous studies have demonstrated that burkholderia pseudomallei secretes protease, lipase, and phospholipase c (plc) into the extracellular milieu, but their mechanisms of secretion and roles in pathogenesis have not been elucidated. in this study, we isolated and characterized 29 transposon mutants unable to secrete protease, lipase, and plc. | 1999 | 10419967 |
| the hamster model of intraperitoneal burkholderia mallei (glanders). | thirty-one female syrian hamsters (mesocricetus auratus) were inoculated intraperitoneally with a lethal dose of burkholderia mallei (budapest strain). hamsters were killed postinoculation on days 0 through 6. lesions were first noted in the spleens on postinoculation day 1, and in mediastinal and mesenteric lymph nodes, mediastinum, liver, and bone marrow on day 2. lesions were present in the lung and submandibular lymph nodes on day 3, and in the brain on day 5. the characteristic histopatholo ... | 1999 | 10421094 |
| [burkholderia mallei and burkholderia pseudomallei. study of immuno- and pathogenesis of glanders and melioidosis. heterologous vaccines]. | parameters of the infectious activity of b.mallei and b.pseudomallei for animals of various species were determined. pathomorphological characteristics of the process of malleus and melioidosis were studied on golden hamsters, mice, guinea pigs, rats and monkeys. tularemia, plague and salmonellosis vaccines were shown to have protective effects in experimental malleus and melioidosis. an insignificant cross immune response between the malleus and melioidosis pathogens was observed. | 1999 | 10422574 |
| guanidine hydrochloride-induced denaturation of pseudomonas cepacia lipase. | the guanidine hydrochloride-induced denaturation of pseudomonas cepacia lipase (pcl) was studied at ph 7 by monitoring the changes in the fluorescence and circular dichroism of the enzyme. the denaturation was irreversible as a whole, and the addition of ca2+ ions decreased the velocity of the denaturation. the denaturation process was well explained consistently by a two-step mechanism, as follows: [see equation in text] where n is the native state of pcl, d(i) an intermediate denatured-state w ... | 1999 | 10423533 |
| characterization of antarctic hydrocarbon-degrading bacteria capable of producing bioemulsifiers. | during screening for biosurfactant-producing, n-alkane-degrading marine bacteria, two heterotrophic bacterial strains were isolated from enriched mixed cultures, obtained from terra nova bay (ross sea, antarctica) by using aliphatic and artomatic hydrocarbons as the principal carbon source. these gram-positive, aerobic, cocci-shaped bacteria use a various number of organic compounds, including aliphatic hydrocarbons, volatile fatty acids, and biphenyl. during cultivation on n-alkanes as sole sou ... | 1999 | 10423744 |
| anaerobic mineralization of quaternary carbon atoms: isolation of denitrifying bacteria on dimethylmalonate. | the microbial capacity to degrade simple organic compounds with quaternary carbon atoms was demonstrated by enrichment and isolation of five denitrifying strains on dimethylmalonate as the sole electron donor and carbon source. quantitative growth experiments showed a complete mineralization of dimethylmalonate. according to phylogenetic analysis of the complete 16s rrna genes, two strains isolated from activated sewage sludge were related to the genus paracoccus within the alpha-proteobacteria ... | 1999 | 10427013 |
| regiospecificity of dioxygenation of di- to pentachlorobiphenyls and their degradation to chlorobenzoates by the bph-encoded catabolic pathway of burkholderia sp. strain lb400. | burkholderia sp. strain lb400 is one of the most potent aerobic polychlorobiphenyl (pcb)-degrading microorganisms that have been characterized. its pcb-dioxygenating activity originates predominantly or exclusively from the biphenyl dioxygenase encoded by its bph gene cluster. analysis of the dioxygenation products of several di- to pentachlorinated biphenyls formed by this enzyme revealed a complex dependence of the regiospecificity and the yield of dioxygenation on the substitution patterns of ... | 1999 | 10427057 |
| soil bacterial community shift correlated with change from forest to pasture vegetation in a tropical soil. | the change in vegetative cover of a hawaiian soil from forest to pasture led to significant changes in the composition of the soil bacterial community. dnas were extracted from both soil habitats and compared for the abundance of guanine-plus-cytosine (g+c) content, by analysis of abundance of phylotypes of small-subunit ribosomal dna (ssu rdna) amplified from fractions with 63 and 35% g+c contents, and by phylogenetic analysis of the dominant rdna clones in the 63% g+c content fraction. all thr ... | 1999 | 10427058 |
| cystic fibrosis: an inherited susceptibility to bacterial respiratory infections. | cystic fibrosis is a severe monogenic disorder of ion transport in exocrine glands. the basic defect predisposes to chronic bacterial airway infections with staphylococcus aureus, haemophilus influenzae, pseudomonas aeruginosa and burkholderia cepacia. the pseudomonas infections in cystic fibrosis are a paradigm of how versatile environmental bacteria can conquer, adapt and persist in an atypical habitat and successfully evade defence mechanisms and chemotherapy in a susceptible host. regular ch ... | 1999 | 10431168 |
| melioidosis with adrenal gland abscess. | we report a case of melioidosis with left adrenal gland abscess in a 51-year-old man from taiwan who traveled to rangoon, burma for a four-day tour on july 15, 1997. the patient developed fever and left upper abdominal pain upon returning to taiwan on july 19, 1997. ten days after returning to taiwan, he was admitted to chang gung memorial hospital in keelung, taiwan and blood culture on admission was positive for burkholderia pseudomallei. computerized tomography of the abdomen revealed left ad ... | 1999 | 10432051 |
| the effect of lipids on bongkrekic (bongkrek) acid toxin production by burkholderia cocovenenans in coconut media. | tempe bongkrek is an indonesian food made by fermentation of coconut presscake or coconut milk residue rhizopus oligosporus. consumption of tempe bongkrek is associated with a food-borne human intoxication and significant numbers of deaths annually. the bacterium burkholderia cocovenenans, which is the causative organism, produces two toxins, toxoflavin and bongkrekic acid (also commonly referred to as bongkrek acid). the reasons why these poisonings occur only in a very limited number of foods ... | 1999 | 10435074 |
| intracellular survival and saprophytic growth of isolates from the burkholderia cepacia complex in free-living amoebae. | members of the taxonomically diverse burkholderia cepacia complex have become a major health risk for patients with cystic fibrosis (cf). although patient-to-patient transmission of b. cepacia strains has been well-documented, very little is known about possible vehicles of transmission and reservoirs for these micro-organisms. in this work, it is shown that strains of the b. cepacia complex can survive within different isolates of the genus acanthamoeba. trophozoites containing bacteria develop ... | 1999 | 10439391 |
| [non fermentative gram negative bacilli isolated in a hospital laboratory]. | to report a prospective study on non fermentative gramnegative bacilli, excluded pseudomonas aeruginosa, isolated at dr. julio c. perrando hospital in resistencia (argentina). the goal of this study was to know their frequency and antimicrobial susceptibility. | 1999 | 10439535 |
| comparative in vitro activity of gatifloxacin against stenotrophomonas maltophilia and burkholderia species isolates including evaluation of disk diffusion and e test methods. | the in vitro activity of gatifloxacin, a new fluoroquinolone, was compared to that of five other fluoroquinolones against 105 stenotrophomonas maltophilia isolates and 52 burkholderia spp. isolates. the gatifloxacin mics were determined using the broth microdilution method and the e test (ab biodisk, sweden); these methods were compared for test accuracy, and 5 microg disk zone diameters were compared for interpretive accuracy using the standardized disk diffusion method. in terms of potency, ga ... | 1999 | 10442421 |
| conserved and hybrid meta-cleavage operons from pah-degrading burkholderia rp007. | we have compared the sequence and gene order of meta-cleavage pathway operons from alpha- and gamma-subgroups of the proteobacteria with operons from burkholderia sp. strain rp007 which belongs to the beta-subgroup of the proteobacteria. burkholderia rp007 was isolated for its ability to degrade phenanthrene and contains two meta-cleavage operons. one exhibits a comparable gene order to previously characterised gamma-subgroup proteobacterial (pseudomonas) meta operons, whilst the other has disti ... | 1999 | 10448110 |
| snping away at innate immunity. | | 1999 | 10449427 |
| association of mannose-binding lectin gene heterogeneity with severity of lung disease and survival in cystic fibrosis. | mannose-binding lectin (mbl) is a key factor in innate immunity, and lung infections are the leading cause of morbidity and mortality in cystic fibrosis (cf). accordingly, we investigated whether mbl variant alleles, which are associated with recurrent infections, might be risk factors for cf patients. in 149 cf patients, different mbl genotypes were compared with respect to lung function, microbiology, and survival to end-stage cf (death or lung transplantation). the lung function was significa ... | 1999 | 10449435 |
| evaluation of three oligonucleotide primer sets in pcr for the identification of burkholderia cepacia and their differentiation from burkholderia gladioli. | to evaluate three oligonucleotide primer pairs--two specific for 16s and 23s rrna sequences of burkholderia cepacia, and the third specific for internal transcribed spacer region of 16s-23s sequences of b gladioli--for the identification and differentiation of reference and clinical strains of these and other species. | 1999 | 10450174 |
| spinal cord disease due to melioidosis. | | 1999 | 10450444 |
| two novel mutations in a cystic fibrosis patient of chinese origin. | cystic fibrosis is rare in non-caucasian populations, and in such populations little is known about the spectrum of mutations and polymorphisms in the cftr gene. we studied a 23-year-old patient of chinese ethnicity with sweat chloride values of 104 mm/l, pancreatic sufficiency, an fev1 60% of normal, sputum cultures positive for staphylococcus aureus and burkholderia cepacia, and a history of allergic bronchopulmonary aspergillosis. genetic screening for 31 common cftr mutations was negative, l ... | 1999 | 10453741 |
| role of ornibactin biosynthesis in the virulence of burkholderia cepacia: characterization of pvda, the gene encoding l-ornithine n(5)-oxygenase. | burkholderia cepacia is a frequent cause of respiratory infections in cystic fibrosis patients. b. cepacia has been shown to produce at least four siderophores which may play a role in the virulence of this organism. to characterize genes involved in the synthesis of siderophores, tn5-ot182 mutants were isolated in strain k56-2, which produces two siderophores, salicylic acid (sa) and ornibactins. two mutants were characterized that did not produce zones on chrome azurol s agar in a commonly use ... | 1999 | 10456885 |
| enantioselective recognition mechanism of secondary alcohol by surfactant-coated lipases in nonaqueous media. | the enantioselective recognition mechanism of secondary alcohol by lipases originated from candida rugosa and pseudomonas cepacia was elucidated on the basis of the kinetic study of the esterification of alcohol with lauric acid in isooctane. to obtain inherent kinetic parameters, we utilized a surfactant-coated lipase whose conformation is considered to be an "open" form in a homogeneous organic solvent. based on the experimental results, the enantioselectivity of lipases was found to be derive ... | 1999 | 10458745 |
| a deadly thorn: a case of imported melioidosis. | | 1999 | 10459942 |
| nmr characterization of substrate binding in the phthalate dioxygenase system. | the paramagnetic enhancements in the nmr relaxation rates for the fluorine in fluorophthalates have been used to determine the position of the phthalate with respect to the mononuclear metal ion in native and metal-substituted derivatives of phthalate dioxygenase (pdo). these studies show directly that the substrate interacts with the mononuclear metal of pdo and provide the first structural characterization of this interaction. with a molecular mass of 200 kda, pdo is one of the largest protein ... | 1999 | 10460160 |
| electron paramagnetic resonance measurements of the ferrous mononuclear site of phthalate dioxygenase substituted with alternate divalent metal ions: direct evidence for ligation of two histidines in the copper(ii)-reconstituted protein. | the metalloenzyme phthalate dioxygenase (pdo) contains two iron-based sites. a rieske-type [2fe-2s] cluster serves as an electron-transferring cofactor, and a mononuclear iron site is the putative site of substrate oxygenation. a reductase, which contains fmn and a plant-type [2fe-2s] ferredoxin domain, transfers electrons from nadh to the rieske center. any of the metal ions, fe(ii), cu(ii), co(ii), mn(ii), and zn(ii), can be used to populate the mononuclear site, but only fe(ii) is competent f ... | 1999 | 10460161 |
| imipenem for the treatment of melioidosis. | melioidosis is a protean disease caused by burkholderia pseudomallei. it is rare in the uk and is generally only seen in patients with a travel history to endemic areas such as thailand, singapore and malaysia. cases may present with disseminated bacteraemic, non-disseminated bacteraemic, multi-focal bacteraemic or localized disease. subclinical infections also occur. following acquisition of the organism a patient may remain asymptomatic for several years before infection becomes clinically app ... | 1999 | 10461846 |
| nosocomial gram-negative bacteremia in critically ill patients: epidemiologic characteristics and prognostic factors in 147 episodes. | although gram-positive organisms are the most common causes of nosocomial bloodstream infections, gram-negative bacteremia carries higher risks of severe sepsis, septic shock, and death among critically ill patients in intensive care units (icus). we performed a prospective epidemiologic analysis of nosocomial gram-negative bacteremia episodes among icu patients and sought to identify risk factors for mortality among these patients. all episodes of nosocomial gram-negative bacteremia documented ... | 1999 | 10462994 |
| a simplified subtractive hybridization protocol used to isolate dna sequences specific to xylella fastidiosa. | a simplified protocol of subtractive hybridization based on the technique of l. m. kunkel, a. p. monaco, w. middlesworth, h. d. ochs & s. a. latt (1985, proc natl acad sci usa, 82, 4778-4782) was used to obtain dna sequences specific to xylella fastidiosa isolated from diseased citrus plants. as a driver, dna extracted from bacteria showing different degrees of relatedness was used: xy. fastidiosa 788 isolated from another host (plum), xanthomonas campestris pv. campestris and burkholderia gladi ... | 1999 | 10463163 |
| [case of melioidosis associated with acute empyema and cellulitis of the leg]. | | 1999 | 10465956 |
| epidemiology of burkholderia pseudomallei in thailand. | the distribution of burkholderia pseudomallei in soil collected from four regions of thailand and the frequency of b. pseudomallei infections in patients attending government hospitals throughout thailand in 1997 were surveyed. a total of 3,585 soil samples collected from 896 sites in four regions of thailand were cultured for b. pseudomallei using selective enrichment broth and modified ashdown's agar. the organism was recovered in 4.4%, 6.1%, 20.4%, and 5.9% of the soil samples collected from ... | 1999 | 10466977 |
| in vitro activity of newer broad spectrum beta-lactam antibiotics against enterobacteriaceae and non-fermenters: a report from austrian intensive care units. austrian carbapenem susceptibility surveillance group. | we compared the in vitro activity of broad spectrum beta-lactam antibiotics against 573 gram-negative isolates (enterobacteriaceae and non-fermenters) collected between november 1996 and may 1997 from 9 laboratories serving intensive care units throughout austria. mic's (minimal inhibitory concentration) were obtained with the e-test for meropenem, imipenem, ceftazidime, cefepime, cefpirome and piperacillin/tazobactam. pseudomonas aeruginosa was the most frequently isolated organism (22%), follo ... | 1999 | 10467641 |
| cardiac cirrhosis with cellulitis caused by burkholderia cepacia bacteremia. | | 1999 | 10476761 |
| endemic burkholderia cepacia bacteraemia: clinical features and antimicrobial susceptibilities of isolates. | burkholderia cepacia has emerged as a nosocomial pathogen, causing numerous outbreaks, particularly among cystic fibrosis (cf) patients. reports of clinical features of endemic b. cepacia bacteraemia in non-cf patients are rare. twenty-five patients with b. cepacia bacteraemia were matched with 25 controls with nosocomial escherichia coli bacteraemia at china medical college hospital, taichung, taiwan, over a period of 3 y. case-patients included 16 men and 9 women, from 13 to 75 y. all had seve ... | 1999 | 10482060 |
| lipopolysaccharide chemotypes of burkholderia cepacia. | burkholderia cepacia is an important pathogen in patients with cystic fibrosis (cf) and much is now known of its epidemiology. in contrast, its virulence mechanisms are poorly understood. the lipopolysaccharide (lps) of b. cepacia, a well-recognised virulence factor of other gram-negative bacteria, is known to be strongly endotoxic in vitro. the aim of this study was to observe if there were any links between the structure of b. cepacia lps and virulence. this has been investigated by polyacryla ... | 1999 | 10482293 |
| development of rrna-based pcr assays for identification of burkholderia cepacia complex isolates recovered from cystic fibrosis patients. | pcr assays targeting rrna genes were developed to identify species (genomovars) within the burkholderia cepacia complex. each assay was tested with 177 bacterial isolates that also underwent taxonomic analysis by whole-cell protein profile. these isolates were from clinical and environmental sources and included 107 b. cepacia complex strains, 23 burkholderia gladioli strains, 20 ralstonia pickettii strains, 10 pseudomonas aeruginosa strains, 8 stenotrophomonas maltophilia strains, and 9 isolate ... | 1999 | 10488171 |
| capillary electrophoresis-single-strand conformation polymorphism analysis for rapid identification of pseudomonas aeruginosa and other gram-negative nonfermenting bacilli recovered from patients with cystic fibrosis. | we used capillary electrophoresis-single-strand conformation polymorphism (ce-sscp) analysis of pcr-amplified 16s rrna gene fragments for rapid identification of pseudomonas aeruginosa and other gram-negative nonfermenting bacilli isolated from patients with cystic fibrosis (cf). target sequences were amplified by using forward and reverse primers labeled with various fluorescent dyes. the labeled pcr products were denatured by heating and separated by capillary gel electrophoresis with an autom ... | 1999 | 10488211 |
| melioidosis, an environmental and occupational hazard in thailand. | melioidosis is a tropical environmental hazard that causes acute and chronic pulmonary disease, abscesses of the skin and internal organs, meningitis, brain abscess and cerebritis, and acute fulminant rapidly fatal sepsis. it is more common among adults, individuals with diabetes, and individuals with chronic renal disease, but it can occur in normal hosts and children. burkholderia pseudomellei is the most prevalent cause of community-acquired pneumonia, liver and splenic abscess, and sepsis in ... | 1999 | 10495639 |
| mediation of attachment of burkholderia pseudomallei to human pharyngeal epithelial cells by the asialoganglioside gm1-gm2 receptor complex. | melioidosis is the term given to any infection caused by burkholderia pseudomallei. this bacteria is one of the important causative agents of life-threatening pulmonary infections in the tropical and subtropical areas. the initiation of respiratory infections is attachment of this bacteria to pharyngeal cells. the precise mechanism of attachment of b. pseudomallei is not known. in this study, we found that asialoganglioside gm1 at concentrations of 25, 12.5, and 5 microg/ml significantly decreas ... | 1999 | 10497993 |
| cloning and characterization of a cryptic haloacid dehalogenase from burkholderia cepacia mba4. | burkholderia cepacia mba4 has been shown to produce a single dehalogenase batch culture. moreover, other cryptic dehalogenases were also detected when the cells were grown in continuous culture. in this paper, we report the cloning and characterization of one of the cryptic dehalogenases in mba4. this cryptic haloacid dehalogenase, designated chd1, was expressed constitutively in escherichia coli. this recombinant chd1 had a relative molecular weight of 58,000 and existed predominantly as a dime ... | 1999 | 10498712 |
| characterization of the phthalate permease ophd from burkholderia cepacia atcc 17616. | the ophd gene, encoding a permease for phthalate transport, was cloned from burkholderia cepacia atcc 17616. expression of the gene in escherichia coli results in the ability to transport phthalate rapidly into the cell. uptake inhibition experiments show that 4-hydroxyphthalate, 4-chlorophthalate, 4-methylphthalate, and cinchomeronate compete for the phthalate permease. an ophd knockout mutant of 17616 grows slightly more slowly on phthalate but is still able to take up phthalate at rates equiv ... | 1999 | 10498738 |
| combination antimicrobial therapy of acute burkholderia pseudomallei infection in a mouse model. | burkholderia pseudomallei is the causative agent of melioidosis, a disease endemic in tropical and subtropical regions of south-east asia and northern australia. antimicrobial therapy regimens for treatment of acute septicemic melioidosis are of variable efficacy. ceftazidime is the current antibiotic of choice and is commonly administered with other agents such as cotrimoxazole or doxycycline. the emergence of resistant strains of b. pseudomallei and the persistence of high mortality rates prom ... | 1999 | 10503275 |
| the effect of human lactoferrin on the mics of doxycycline and rifampicin for burkholderia cepacia and pseudomonas aeruginosa strains. | the presence of lactoferrin at the concentration found in cystic fibrosis (cf) sputum (0.9 g/l) reduced mics and mbcs of doxycycline for burkholderia cepacia and pseudomonas aeruginosa strains. mics for b. cepacia fell by 32- to 64-fold, from highly resistant to clinically achievable values. rifampicin mics for b. cepacia strains were reduced by lactoferrin and for some strains mbcs were reduced. these findings suggest new therapeutic approaches to infections and question the relevance of standa ... | 1999 | 10511407 |
| cost of care for individuals with cystic fibrosis: a regression approach to determining the impact of recombinant human dnase. | we estimated direct medical costs of care and important determinants of the costs in patients with cystic fibrosis (cf), including therapy with recombinant human dnase (rhdnase). costs were estimated with resource use data from the epidemiologic study of cystic fibrosis. ordinary least squares regression was used to determine the effect of clinical and demographic variables on individual cost of care. the estimated cost of caring for 303 patients in alberta was $2,279,801 in 1996. the mean cost ... | 1999 | 10512065 |
| cystic fibrosis: inflammatory response to infection with burkholderia cepacia and pseudomonas aeruginosa. | pulmonary colonization by burkholderia cepacia in cystic fibrosis (cf) may be associated with enhanced deterioration of pulmonary function. this may be due to a more florid host inflammatory response than in colonization by pseudomonas aeruginosa, leading to greater lung injury. circulating markers of inflammation were determined during infective exacerbations and periods of clinical stability in an 18 month prospective study in adults with cf colonized by p. aeruginosa (n=41). b. cepacia (n=13) ... | 1999 | 10515426 |
| transformation of polychlorinated biphenyls by a novel bpha variant through the meta-cleavage pathway. | the transformation of 20 polychlorinated biphenyls (pcbs) through the meta-cleavage pathway by recombinant escherichia coli cells expressing the bphefgbc locus from burkholderia cepacia lb400 and the bpha genes from different sources was compared. the analysis of pcb congeners for which hydroxylation was observed but no formation of the corresponding yellow meta-cleavage product demonstrated that only lightly chlorinated congeners including one tetrachlorobiphenyl (2,2',4,4'-cb) were transformed ... | 1999 | 10518716 |
| convenient and versatile dna extraction using agarose plugs for ribotyping of problematic bacterial species. | we describe a convenient, versatile and safe method for preparing bacterial dna for ribotyping analysis. in this method, extraction of bacterial dna from salmnonella typhi and burkholderia pseudomallei. and subsequent restriction endonuclease digestion, was performed in agarose blocks/plugs thus minimizing shearing and loss of dna, problems commonly associated with liquid phase phenol extraction. digested dna in the plugs was then electrophoresed directly, transferred to nylon membranes and hybr ... | 1999 | 10520586 |
| rapid identification of burkholderia pseudomallei in blood cultures by a monoclonal antibody assay. | burkholderia pseudomallei is the causative agent of melioidosis. in northeast thailand, this gram-negative bacterium is a major cause of mortality from septicemia. the definitive diagnosis of this disease is made by bacterial culture. in this study, we produced a monoclonal antibody (mab) specific to the 30-kda protein of b. pseudomallei by in vivo and in vitro immunization of balb/c mice with a crude culture filtrate antigen. the mab could directly agglutinate with all 243 clinical isolates of ... | 1999 | 10523570 |
| cloning and characterization of a nonhemolytic phospholipase c gene from burkholderia pseudomallei. | we cloned and characterized a phosphatidylcholine-hydrolyzing phospholipase c (pc-plc) gene from burkholderia pseudomallei. dna sequence analysis of the gene indicated an open reading frame coding for 700 amino acids with a 34-amino-acid signal peptide. when cleaved, this yields a secreted 73-kda mature protein. the deduced amino acid sequence exhibited 48% similarity to that of a nonhemolytic plc from pseudomonas aeruginosa. the expressed pc-plc was heat stable, nonhemolytic for sheep erythrocy ... | 1999 | 10523590 |
| melioidosis in southern vietnam: clinical surveillance and environmental sampling. | from 1992-1998, burkholderia pseudomallei was isolated from only 9 (0.25%) of 3653 cultures of blood from febrile patients admitted to the centre for tropical diseases in ho chi minh city, an infectious disease referral center for southern vietnam. soil was sampled from 407 sites in 147 rice fields along the 5 major roads radiating from ho chi minh city. b. pseudomallei was isolated from 73 sites (18%) in 39 rice fields (27%), but only 15 (21%) of the 71 isolates from 9 (6%) of 147 fields were t ... | 1999 | 10524986 |
| regulation of expression of the nonhemolytic phospholipase c of burkholderia cepacia. | burkholderia cepacia is an opportunistic pathogen that causes serious pulmonary infections in cystic fibrosis patients. we have purified and partially characterized one potential virulence factor for the organism-a nonhemolytic phospholipase c-and we studied the effect of iron restriction and choline and phosphate concentrations on the expression of phospholipase c. iron limitation did not affect expression, the effect of choline was variable, and high phosphate concentrations repressed expressi ... | 1999 | 10525838 |
| [elastase activity of gram-negative non-fermenting bacteria and two variations of a simple method for their detection]. | the ability to produce elastase was examined in 1,168 strains of 11 representative genera of gram-negative non-fermenting bacteria isolated from weakened patients in the course of eight years. detection of the elastase activity was performed parallelly using the conventional method and its pulverisation variant. the difference between the results accomplished by the classical method and its pulverisation variant (53.9% versus 60.5% detected elastase-positive strains) was highly significant (p < ... | 1999 | 10528445 |
| differences in genomic macrorestriction patterns of arabinose-positive (burkholderia thailandensis) and arabinose-negative burkholderia pseudomallei. | we reported previously two biochemically and antigenically distinct biotypes of burkholderia pseudomallei. these two distinct biotypes could be distinguished by their ability to assimilate l-arabinose. some b. pseudomallei isolated from soil samples could utilize this substrate (ara+), whereas the other soil isolates and all clinical isolates could not (ara-). only the ara isolates were virulent in animals and reacted with monoclonal antibody directed at the surface envelope, most likely the exo ... | 1999 | 10529102 |
| changing epidemiology of infections in patients with neutropenia and cancer: emphasis on gram-positive and resistant bacteria. | over the past 3 decades, considerable changes have occurred in the types of bacteria causing infection in febrile patients with neutropenia and cancer. twenty years ago, gram-negative bacteria caused approximately 70% of bloodstream infections. as a probable consequence of long-dwelling intravascular devices, fluoroquinolone prophylaxis, and high-dose chemotherapy-induced mucositis, there has been a shift toward gram-positive coccal bacteremia. in most centers today, approximately 70% of bactere ... | 1999 | 10530434 |
| occurrence and expression of glutathione-s-transferase-encoding bphk genes in burkholderia sp. strain lb400 and other biphenyl-utilizing bacteria. | the gene bphk of burkholderia sp. strain lb400 has previously been shown to be located within the bph locus, which specifies the degradation of biphenyl (bp) and chlorobiphenyls, and to encode a glutathione s-transferase (gst) which accepts 1-chloro-2,4-dinitrobenzene (cdnb) as substrate. the specific physiological role of this gene is not known. it is now shown that the gene is expressed in the parental organism and that gst activity is induced more than 20-fold by growth of the strain on bp re ... | 1999 | 10537204 |
| bacterial populations in an anthropogenically disturbed stream: comparison of different seasons. | abstract to determine the effects of environmental changes on stream bacterial populations, assemblage- and population-level measurements were compared between an anthropogenically disturbed stream and an undisturbed reference stream during different seasons. physical and chemical variables monitored at two disturbed sites from a stream affected by multiple environmental perturbations confirmed discernibly different water quality from three reference sites: two from an adjacent, undisturbed wate ... | 1999 | 10541785 |
| soil type and maize cultivar affect the genetic diversity of maize root-associated burkholderia cepacia populations. | abstract burkholderia cepacia populations associated with the zea mays root system were investigated to assess the influence of soil type, maize cultivar, and root localization on the degree of their genetic diversity. a total of 180 b. cepacia isolates were identified by restriction analysis of the amplified 16s rdna (ardra technique). the genetic diversity among b. cepacia isolates was analyzed by the random amplified polymorphic dna (rapd) technique, using the 10-mer primer ap5. the analysis ... | 1999 | 10541789 |
| isolation of polymyxin b-susceptible mutants of burkholderia pseudomallei and molecular characterization of genetic loci involved in polymyxin b resistance. | burkholderia pseudomallei is a gram-negative bacterium that causes the disease known as melioidosis. this pathogen is endemic to southeast asia and northern australia and is particularly problematic in northeastern thailand. it has been previously reported that b. pseudomallei is resistant to the killing action of cationic antimicrobial peptides, including human neutrophil peptide, protamine sulfate, poly-l-lysine, magainins, and polymyxins. recently, we have also found that the virulent clinica ... | 1999 | 10543742 |
| in vitro susceptibilities of burkholderia mallei in comparison to those of other pathogenic burkholderia spp. | the in vitro antimicrobial susceptibilities of isolates of burkholderia mallei to 16 antibiotics were assessed and compared with the susceptibilities of burkholderia pseudomallei and burkholderia cepacia. the antibiotic susceptibility profile of b. mallei resembled that of b. pseudomallei more closely than that of b. cepacia, which corresponds to their similarities in terms of biochemistry, antigenicity, and pathogenicity. ceftazidime, imipenem, doxycycline, and ciprofloxacin were active against ... | 1999 | 10543761 |
| conservation of the pyrrolnitrin biosynthetic gene cluster among six pyrrolnitrin-producing strains. | the prnabcd gene cluster from pseudomonas fluorescens encodes the biosynthetic pathway for pyrrolnitrin, a secondary metabolite derived from tryptophan which has strong anti-fungal activity. we used the prn genes from p. fluorescens strain bl915 as a probe to clone and sequence homologous genes from three other pseudomonas strains, burkholderia cepacia and myxococcus fulvus. with the exception of the prna gene from m. fulvus59% similar among the strains, indicating that the biochemical pathway f ... | 1999 | 10547442 |
| rapid identification of burkholderia pseudomallei in blood cultures by latex agglutination using lipopolysaccharide-specific monoclonal antibody. | melioidosis, an infection caused by burkholderia pseudomallei, is endemic in southeast asia. the septicemic form of melioidosis is the leading cause of death due to community-acquired bacteremia in the northeastern part of thailand. the delay in isolation and identification of the causative organism is a major contributing factor to the high mortality. the present study describes the evaluation of a latex agglutination test for rapid identification of the bacteria directly from blood cultures. t ... | 1999 | 10548306 |
| identification of burkholderia species and genomovars from cystic fibrosis patients by aflp fingerprinting. | aflp is a genomic fingerprinting technique based on the selective amplification of restriction fragments from a total double-digest of genomic dna. the applicability of this method to differentiate between species and genomovars of the genus burkholderia was tested, with particular emphasis on taxa occurring in cystic fibrosis patients. in this study, 78 well-characterized strains and field isolates were investigated by two methods of aflp fingerprinting. in the manual procedure, a radioactively ... | 1999 | 10555347 |
| burkholderia cepacia in cystic fibrosis. variable disease course. | variable clinical course has been reported with the acquisition of burkholderia cepacia in patients who have cystic fibrosis (cf). we hypothesized that the perceived worsening with b. cepacia may reflect the underlying severity of pulmonary disease at the time of acquisition. to test this hypothesis, we matched cf patients colonized with b. cepacia with cf patients not colonized with the organism. two-year pre- and postacquisition data and long-term data were compared. patients were matched for ... | 1999 | 10556123 |
| elevated plasma concentrations of interferon (ifn)-gamma and the ifn-gamma-inducing cytokines interleukin (il)-18, il-12, and il-15 in severe melioidosis. | interferon (ifn)-gamma plays an important role in the pathogenesis of sepsis. production of ifn-gamma is stimulated by synergistic effects of interleukin (il)-18, il-12, and il-15. to investigate the regulation of ifn-gamma production during severe gram-negative infection, the plasma concentrations of ifn-gamma, il-18, il-12, and il-15 were measured in 83 patients with suspected melioidosis. the diagnosis was confirmed in 62 patients, 31 of whom had blood cultures positive for burkholderia pseud ... | 1999 | 10558944 |
| molecular epidemiological investigation using a randomly amplified polymorphic dna assay of burkholderia cepacia isolates from nosocomial outbreaks. | we experienced two burkholderia cepacia outbreaks over a 1-year period. during this period, 28 b. cepacia isolates were obtained from clinical specimens, and 2 were obtained from environmental specimens (i.e., from a nebulizer solution and a nebulizer tube). these 30 isolates were subjected to the pcr-based randomly amplified polymorphic dna (rapd) assay as well as to pulsed-field gel electrophoresis (pfge). in the first outbreak, in which eight patients hospitalized in the trauma and critical c ... | 1999 | 10565889 |
| successful treatment with methylprednisolone pulse therapy for a life-threatening pulmonary insufficiency in a patient with chronic granulomatous disease following pulmonary invasive aspergillosis and burkholderia cepacia infection. | a 14-year-old boy with x-linked chronic granulomatous disease developed severe invasive pulmonary aspergillosis. he was treated with itraconazole and amphotericin b. however, he deteriorated with progressive pulmonary lesions. burkholderia cepacia was isolated from his bronchoalveolar lavage. finally, he was given granulocyte transfusions. following this procedure, his condition rapidly worsened leading to respiratory failure. his lung biopsy demonstrated organizing pneumonia at his right middle ... | 1999 | 10575344 |
| aminoglycoside and macrolide resistance in burkholderia pseudomallei. | | 1999 | 10577350 |
| recombinant microbial lipases for biotechnological applications. | lipases, mainly of microbial origin, represent the most widely used class of enzymes in biotechnological applications and organic chemistry. modern methods of genetic engineering combined with an increasing knowledge of structure and function will allow further adaptation to industrial needs and exploration of novel applications. production of such tailored lipases requires their functional overexpression in a suitable host. hence, this article describes the functional heterologous production of ... | 1999 | 10579516 |
| novel screening methods--the key to cloning commercially successful biocatalysts. | providing sufficient biocatalyst to support the demands of multi tonne product supply can be problematical. here we describe how screening for and cloning a gamma-lactamase overcame biocatalyst supply issues, and greatly improved the actual biocatalytic process. the isolation of an expressing gamma-lactamase clone from a gene library necessitated a combination of classical molecular biology techniques together with innovative screening methods to identify a functional clone. once isolated the en ... | 1999 | 10579521 |
| visualization of enzyme-catalyzed reactions using ph indicators: rapid screening of hydrolase libraries and estimation of the enantioselectivity. | the use of ph indicators to monitor hydrolase-catalyzed reactions is described. the formation of acid following an enzyme-mediated hydrolysis causes a drop in the ph that can be visualized by a change in the color of the indicator-containing solution. the best indicators are those showing a color transition within the operational ph range of the hydrolases, like bromothymol blue and phenol red. the enantioselectivity of lipases and esterases can be estimated using single isomers under the same c ... | 1999 | 10579524 |
| sequence heterogeneity of the ferripyoverdine uptake (fpva), but not the ferric uptake regulator (fur), genes among strains of the fluorescent pseudomonads pseudomonas aeruginosa, pseudomonas aureofaciens, pseudomonas fluorescens and pseudomonas putida. | pseudomonas aeruginosa, pseudomonas aureofaciens, pseudomonas fluorescens and pseudomonas putida are of importance to medicine, agriculture and biocycling. these microbes acquire ferric ion via the use of the siderophores pyochelin and the family known as the pyoverdines or pseudobactins. the ferric uptake regulator (fur) gene is responsible, at least in part, for the regulation of siderophore synthesis and uptake in p. aeruginosa. to determine whether the organisms contain single or multiple ho ... | 1999 | 10581691 |
| fluorescent inhibitors for the qualitative and quantitative analysis of lipolytic enzymes. | we report on the determination of active enzyme components in pure and crude lipases, using fluorescent inhibitors for covalent modification and visualization of the enzymatically active proteins. lipase-specific compounds are triacylglycerol analogs, namely 1,2(2, 3)-di-o-alkylglyceroalkylphosphonic acid-p-nitrophenyl esters, containing a fluorescent substituent bound to the omega-end of an alkyl chain. inhibitors derived from single-chain alcohols, such as p-nitrophenyl esters of fluorescent a ... | 1999 | 10585746 |
| antilipopolysaccharide ii: an antibody protective against fatal melioidosis. | this was a study of igg antibody responses to two s-type lipopolysaccharides (lps i and lps ii) and flagellin of burkholderia pseudomallei in patients with melioidosis. the specificity of these antibodies was 91.7%, 90.3%, and 93.8%, respectively, when compared to responses in a population where the organism is not endemic. only the level of antibody to lps ii (anti-lps ii) was significantly higher in patients who survived than in those who died, as well as in patients with nonsepticemic vs. sep ... | 1999 | 10589895 |
| bacteremic community-acquired pneumonia in an immunocompetent adult due to burkholderia cepacia. | | 1999 | 10593827 |
| antibacterial activities of extracts from nigerian chewing sticks. | ten aqueous extracts from wooden chewing sticks widely used in nigeria for teeth cleaning were studied for antibacterial activities against 25 different bacteria using an agar diffusion assay. the extracts from five sticks, namely garcinia kola, anogeissus leiocarpus, terminalia glaucescens, sorindeia warneckei and vitex doniana, exhibited strong activities against a wide spectrum of bacteria including medically and dentally relevant bacteria. notably, these five chewing stick extracts showed po ... | 1999 | 10594937 |
| current studies on the pathogenesis of melioidosis. | burkholderia pseudomallei is a major cause of bacterial septicemias in many parts of the world, particularly thailand; the known geographic range of the organism appears to be enlarging as awareness of the organism and the disease it causes--melioidosis--increases. b. pseudomallei is intrinsically resistant to most antibiotics, and our knowledge of b. pseudomallei pathogenesis is lacking. thus, the long-term objective of our research is to define at a molecular level the pathogenesis by combinin ... | 1999 | 10594980 |
| [the epidemiology of burkholderia cepacia in cystic fibrosis patients: the initial results of a study including the genotypic typing of the strains isolated]. | | 1999 | 10596443 |