| [analysis of transmission of burkholderia cepacia isolates in an intrahospital by randomly amplified polymorphic dna-pcr method]. | strains of burkholderia cepacia isolated in our hospital from november 1995 to september 1996 were classified with randomly amplified polymorphic dna-pcr (rapd-pcr) and conventional biochemical tests (id test.nf-18, api20ne, and neg combo 4j kit), and intrahospital isolates of b. cepacia were analysed. during the period 28 strains from inpatients and 2 from medical apparatus were isolated. twenty four of 28 (85.7%) were from sputum. in 1996 from january to february, 20 strains were detected from ... | 1998 | 9745217 |
| generation of a reproducible nutrient-depleted biofilm of escherichia coli and burkholderia cepacia. | an in vitro method of growing bacteria as a defined nutrient-depleted biofilm is proposed. the medium was defined nutritionally in terms of the quantitative composition and by the total amount of nutrient required to achieve a defined population size. escherichia coli and burkholderia cepacia were incubated on a filter support placed on a defined volume of solid medium. the change of biomass of the biofilm population was compared with the change in a planktonic culture. the size of the populatio ... | 1998 | 9750276 |
| the diversity of lipases from psychrotrophic strains of pseudomonas: a novel lipase from a highly lipolytic strain of pseudomonas fluorescens. | strains of pseudomonas fluorescens and ps. fragi are the predominant psychrotrophs found in raw milk and may cause spoilage due to the secretion of hydrolytic enzymes such as lipase and protease. the diversity of lipases has been examined in pseudomonas isolates from raw milk which represent different taxonomic groups (phenons). significant diversity was found using both dna hybridization and immunoblotting techniques, which has implications for the development of a diagnostic test. the lipase-e ... | 1998 | 9750283 |
| macrophage-lymphocyte interactions mediate anti-burkholderia pseudomallei activity. | the mechanisms involved in the pathogenesis of melioidosis, caused by the intracellular bacterium burkholderia pseudomallei, are unclear. c57bl/6 mice are resistant to infection, while balb/c mice are highly susceptible. previous studies have demonstrated that peritoneal exudate cell preparations enriched for macrophages are capable of effectively eliminating intracellular pathogens. in this study we present evidence showing that interaction of macrophages with lymphocytes is necessary for effic ... | 1998 | 9753000 |
| [evaluation of antibacterial activities of various antibiotics against glucose non-fermentative gram-negative rods other than pseudomonas aeruginosa]. | mics of piperacillin, sulbactam/cefoperazone, minocycline (mino), gentamicin, amikacin, flomoxef, ceftazidime, cefozopran, cefsulodin and imipenem were determined, against 189 clinical isolated strains of glucose non-fermentative gram-negative rods (nfgnr; acinetobacter baumannii (44), alcaligenes faecalis (5), alcaligenes xylosoxidans (25), burkholderia cepacia (12), chryseobacterium indologenes (23), chryseobacterium meningosepticum (9), pseudomonas fluorescens (8), pseudomonas putida (12), st ... | 1998 | 9755431 |
| hla-dr and -dq associations with melioidosis. | melioidosis is an important infectious disease of southeast asia caused by an intracellular bacterium, burkholderia pseudomallei. cellular immunity is postulated to play important roles in immunity to melioidosis that may influence the severity and clinical outcome of the disease. the present study was undertaken to investigate possible associations of melioidosis with hla class ii alleles. hla typing of hla-drb1, -dqa1, and -dqb1 was performed using polymerase chain reaction and sequence-specif ... | 1998 | 9757914 |
| optimization of differential display of prokaryotic mrna: application to pure culture and soil microcosms. | the differential display (dd) technique, which is widely used almost exclusively for eukaryotic gene discovery, was optimized to detect differential mrna transcription from both pure-culture and soil-derived bacterial rna. a model system which included toluene induction of todc1 in pseudomonas putida f1 was used to optimize the procedure. at 24-h tod induction was determined to be approximately 8 x 10(7) transcripts/microg or 0.08% of the total mrna. the primer concentration, primer length, anne ... | 1998 | 9758787 |
| characterization of genes involved in biosynthesis of a novel antibiotic from burkholderia cepacia bc11 and their role in biological control of rhizoctonia solani. | genetic manipulation of fluorescent pseudomonads has provided major insight into their production of antifungal molecules and their role in biological control of plant disease. burkholderia cepacia also produces antifungal activities, but its biological control activity is much less well characterized, in part due to difficulties in applying genetic tools. here we report genetic and biochemical characterization of a soil isolate of b. cepacia relating to its production of an unusual antibiotic t ... | 1998 | 9758823 |
| evidence for interspecies gene transfer in the evolution of 2,4-dichlorophenoxyacetic acid degraders. | small-subunit ribosomal dna (ssu rdna) from 20 phenotypically distinct strains of 2,4-dichlorophenoxyacetic acid (2,4-d)-degrading bacteria was partially sequenced, yielding 18 unique strains belonging to members of the alpha, beta, and gamma subgroups of the class proteobacteria. to understand the origin of 2,4-d degradation in this diverse collection, the first gene in the 2,4-d pathway, tfda, was sequenced. the sequences fell into three unique classes found in various members of the beta and ... | 1998 | 9758850 |
| burkholderia cepacia. management issues and new insights. | although burkholderia cepacia colonizes a relatively small proportion of individuals with cystic fibrosis (cf), it is associated with significant morbidity and mortality, and has had a profound impact on infection control practices. this article reviews the current understanding of the epidemiology of b. cepacia infection, describes important recent developments in the microbiology and taxonomy of this species, and presents issues that remain obstacles to defining the optimal management of b. ce ... | 1998 | 9759550 |
| agricultural and medical microbiology: a time for bridging gaps. | | 1998 | 9782485 |
| an ultrastructural study of the phagocytosis of burkholderia pseudomallei. | burkholderia pseudomallei causes melioidosis and is believed to be an intracellular pathogen in human and animal disease. the uptake of b. pseudomallei by mouse peritoneal macrophages and cells in tissue culture was examined by electron microscopy. in all the systems studied b. pseudomallei were phagocytosed and apparently inhibited the normal processes of intracellular killing. destruction of the phagosome membrane occurred and the bacteria escaped into the cytoplasm. | 1998 | 9785484 |
| epidemiological surveillance of melioidosis in singapore. | during the period 1989 to 1996, a total of 372 cases of melioidosis, with 147 deaths, were reported, giving a mean annual incidence rate of 1.7 per 100,000 population and a case-fatality rate of 39.5%. majority (89%) of the clinical cases were confirmed by culture of burkholderia pseudomallei, while the others were presumptive cases based on a single blood specimen with an indirect haemaglutination (iha) antibody titre of > or = 1:16. the highest incidence rate was reported in those aged 45 year ... | 1998 | 9791650 |
| ratios of carbon isotopes in microbial lipids as an indicator of substrate usage. | the occurrence and abundance of microbial fatty acids have been used for the identification of microorganisms in microbial communities. however, these fatty acids can also be used as indicators of substrate usage. for this, a systematic investigation of the discrimination of the stable carbon isotopes by different microorganisms is necessary. we grew 11 strains representing major bacterial and fungal species with four different isotopically defined carbon sources and determined the isotope ratio ... | 1998 | 9797266 |
| isolation of burkholderia cepacia by enrichment. | burkholderia cepacia is a recognised cause of respiratory failure in patients with cystic fibrosis. the value of routine use of selective enrichment broth to increase the yield of b cepacia from cystic fibrosis sputa was investigated. two hundred sputa from 86 adult and paediatric patients were cultured onto b cepacia selective agar and also in enrichment broth. the enrichment broths were subcultured after incubation onto b cepacia selective agar. fourteen sputa from eight patients yielded b cep ... | 1998 | 9797739 |
| prevalence of human melioidosis on hainan island in china. | eight human cases of melioidosis were diagnosed at hainan people's hospital over a period of one year. four of the cases were of septicemia form, while the rest were of chronic form with abscesses in different organs. all the isolates were intrinsically resistant to cefazolin, cefuroxime, and gentamicin, while being rather sensitive to ampicillin/sulbactam, ticarcillin/clavulanic acid, and imipenem. a serological survey of exotoxin antibodies at different farms showed that xinglong was a farm se ... | 1998 | 9802565 |
| monomicrobial nonneutrocytic bacterascites due to burkholderia pickettii. | | 1998 | 9820433 |
| subspecific differentiation of burkholderia cepacia isolates in cystic fibrosis. | thirty clinical isolates of burkholderia cepacia from cystic fibrosis (cf) patients in the uk and denmark were characterised, together with other clinical isolates and laboratory strains of b. cepacia, b. gladioli and b. vietnamiensis. outer-membrane protein (omp) profiles were determined, and the organisms were typed genotypically by pulsed-field gel electrophoresis after dna restriction analyses with xbal and drai. this latter method revealed four clusters among the clinical isolates studied; ... | 1998 | 9822299 |
| nosocomial burkholderia cepacia infection and colonization associated with intrinsically contaminated mouthwash--arizona, 1998. | during august 1996-june 1998, 74 patients at two hospitals in arizona had cultures positive for burkholderia cepacia. most isolates were from the respiratory tracts of patients in intensive-care units (icus). because of the large number of b. cepacia isolates, personnel at both hospitals requested the arizona department of health services assist in an investigation. this report summarizes the results of the investigation. | 1998 | 9822365 |
| [database on nucleotide sequences used as primers of microorganisms]. | the data base (db) "primers of microorganisms" for the accumulation and systematization of information on oligonucleotide sequences, used as primers in polymerase chain reaction, has been created. this db includes data on primers for the laboratory diagnostics of 20 bacterial genera (aerococcus, aeromonas, bartonella, borrelia, burkholderia, chlamydia, clostridium, corynebacterium, escherichia, francisella, helicobacter, legionella, listeria, mycobacterium, mycoplasma, salmonella, shigella, stap ... | 1998 | 9825498 |
| biodegradation pathway of 2-chlorodibenzo-p-dioxin and 2-chlorodibenzofuran in the biphenyl-utilising strain jb1. | the biphenyl-utilising burkholderia (previously alcaligenes) strain jb1 is also able to degrade a number of chlorinated dibenzo-p-dioxins and dibenzofurans. in this study, 4-chlorocatechol and a chlorotrihydroxydiphenyl ether were identified as metabolites of 2-chlorodibenzo-p-dioxin. 5-chlorosalicylic acid and a chlorotrihydroxybiphenyl were metabolites of 2-chlorodibenzofuran. these results show that degradation of these compounds follows pathways in which the initial reaction is angular dioxy ... | 1998 | 9828319 |
| characteristics of phenanthrene-degrading bacteria isolated from soils contaminated with polycyclic aromatic hydrocarbons. | ten bacterial strains were isolated from seven contaminated soils by enrichment with phenanthrene as the sole carbon source. these isolates and another phenanthrene-degrading strain were examined for various characteristics related to phenanthrene degradation and their ability to metabolize 12 other polycyclic aromatic hydrocarbons (pah), ranging in size from two to five rings, after growth in the presence of phenanthrene. fatty acid methyl ester analysis indicated that at least five genera (agr ... | 1998 | 9830104 |
| psychrotolerant bacteria isolated from arctic soil that degrade polychlorinated biphenyls at low temperatures | psychrotolerant polychlorinated biphenyl (pcb)-degrading bacteria were isolated at 7 degreesc from pcb-contaminated arctic soil by using biphenyl as the sole organic carbon source. these isolates were distinguished from each other by differences in substrates that supported growth and substrates that were oxidized. 16s ribosomal dna sequences suggest that these isolates are most closely related to the genus pseudomonas. total removal of aroclor 1242, and rates of removal of selected pcb congener ... | 1998 | 9835569 |
| species-specific oligonucleotides for enumeration of pseudomonas putida f1, burkholderia sp. strain js150, and bacillus subtilis atcc 7003 in biodegradation experiments. | species-specific sequences were identified within the v4 variable region of 16s rrna of two bacterial species capable of aromatic hydrocarbon metabolism, pseudomonas putida f1 and burkholderia sp. strain js150, and a third, bacillus subtilis atcc 7003, that can function as a secondary degrader. fluorescent in situ hybridization (fish) with species-specific oligonucleotides was used for direct counting of these species throughout a phenol biodegradation experiment in batch culture. traditional di ... | 1998 | 9835594 |
| dry-season outbreak of melioidosis in western australia. | | 1998 | 9843113 |
| novel organization of the genes for phthalate degradation from burkholderia cepacia dbo1. | burkholderia cepacia dbo1 is able to utilize phthalate as the sole source of carbon and energy for growth. two overlapping cosmid clones containing the genes for phthalate degradation were isolated from this strain. subcloning and activity analysis localized the genes for phthalate degradation to two separate regions on the cosmid clones. analysis of the nucleotide sequence of these two regions showed that the genes for phthalate degradation are arranged in at least three transcriptional units. ... | 1998 | 9851995 |
| rapid identification of burkholderia pseudomallei by latex agglutination based on an exopolysaccharide-specific monoclonal antibody. | we recently identified a constitutively expressed exopolysaccharide of burkholderia pseudomallei which is composed of a unique linear tetrasaccharide repeating unit consisting of three galactose residues and one 3-deoxy-d-manno-2-octulosonic acid residue. in this study we developed a latex agglutination test based on monoclonal antibody 3015, which is specific for this exopolysaccharide, and evaluated this test for rapid identification of b. pseudomallei grown on agar plates. all 74 environmenta ... | 1999 | 9854098 |
| molecular basis for the stabilization and inhibition of 2, 3-dihydroxybiphenyl 1,2-dioxygenase by t-butanol. | the steady-state cleavage of catechols by 2,3-dihydroxybiphenyl 1, 2-dioxygenase (dhbd), the extradiol dioxygenase of the biphenyl biodegradation pathway, was investigated using a highly active, anaerobically purified preparation of enzyme. the kinetic data obtained using 2,3-dihydroxybiphenyl (dhb) fit a compulsory order ternary complex mechanism in which substrate inhibition occurs. the km for dioxygen was 1280 +/- 70 microm, which is at least 2 orders of magnitude higher than that reported fo ... | 1998 | 9857017 |
| isolation and characterisation of disodium (4-amino-4-deoxy-beta-l- arabinopyranosyl)-(1-->8)-(d-glycero-alpha-d-talo-oct-2-ulopyranosylona te)- (2-->4)-(methyl 3-deoxy-d-manno-oct-2-ulopyranosid)onate from the lipopolysaccharide of burkholderia cepacia. | a trisaccharide was isolated from the core oligosaccharide in the lipopolysaccharide (lps) of burkholderia cepacia gifu 645 (atcc 25416, type strain) by methanolysis followed by hplc and saponification. it was identified by ms, methylation analysis and 1h and 13c nmr spectroscopy as disodium (4-amino-4-deoxy-beta-l-arabinopyranosyl)-(1-->8)-(d-glycero- alpha-d-talo-oct-2-ulopyranosylonate)-(2-->4)-(methyl 3-deoxy-d-manno-oct-2-ulopyranosid)onate. in addition to the trisaccharide derivative, meth ... | 1998 | 9861699 |
| genetic and biochemical analyses of the tec operon suggest a route for evolution of chlorobenzene degradation genes. | the teca broad-spectrum chlorobenzene dioxygenase of burkholderia sp. strain ps12 catalyzes the first step in the mineralization of 1,2,4, 5-tetrachlorobenzene. the catabolic genes were localized on a small plasmid that belongs to the incpbeta incompatibility group. pcr analysis of the genetic environment of the tec genes indicated high similarity to the transposon-organized catabolic tcb chlorobenzene degradation genes of pseudomonas sp. strain p51. sequence analysis of the regions flanking the ... | 1999 | 9864349 |
| small genes/gene-products in escherichia coli k-12. | forty-two protein spots of observed m(r) 6-15 kda were resolved by two-dimensional gel electrophoresis, stained by coomassie blue and subjected to edman microsequencing. all of the proteins could be related back to their encoding open reading frames, thereby vindicating the bioinformatic tools currently utilised in their identification. however, only 14/42 gene-products were expressed as annotated. translation was confirmed for 14 open reading frames with no attributed function (ecogene y-entrie ... | 1998 | 9868784 |
| in situ, real-time catabolic gene expression: extraction and characterization of naphthalene dioxygenase mrna transcripts from groundwater. | we developed procedures for isolating and characterizing in situ-transcribed mrna from groundwater microorganisms catabolizing naphthalene at a coal tar waste-contaminated site. groundwater was pumped through 0.22-microm-pore-size filters, which were then frozen in dry ice-ethanol. rna was extracted from the frozen filters by boiling sodium dodecyl sulfate lysis and acidic phenol-chloroform extraction. transcript characterization was performed with a series of pcr primers designed to amplify nah ... | 1999 | 9872763 |
| polycyclic aromatic hydrocarbon degradation by a new marine bacterium, neptunomonas naphthovorans gen. nov., sp. nov. | two strains of bacteria were isolated from creosote-contaminated puget sound sediment based on their ability to utilize naphthalene as a sole carbon and energy source. when incubated with a polycyclic aromatic hydrocarbon (pah) compound in artificial seawater, each strain also degraded 2-methylnaphthalene and 1-methylnaphthalene; in addition, one strain, nag-2n-113, degraded 2,6-dimethylnaphthalene and phenanthrene. acenaphthene was not degraded when it was used as a sole carbon source but was d ... | 1999 | 9872786 |
| structural characterisation of a rhamnan and a fucorhamnan, both present in the lipopolysaccharide of burkholderia vietnamiensis strain lmg 10926. | the polymeric fraction isolated after mild acid hydrolysis of the lipopolysaccharide (lps) from burkholderia vietnamiensis strain lmg 10926 contained l-rhamnose (rha) and d-fucose (fuc). from nmr studies supported by the results of methylation analysis and smith degradation, it could be inferred that the material was probably a mixture of two glycans. one component was a linear rhamnan with a trisaccharide repeating unit (1); the other was a branched fucorhamnan with a tetrasaccharide repeating ... | 1998 | 9874236 |
| flagellin gene variation between clinical and environmental isolates of burkholderia pseudomallei contrasts with the invariance among clinical isolates. | the flagellin gene sequence from a clinical isolate of burkholderia pseudomallei was used to design oligonucleotide primers for pcr/rflp analysis of flagellin gene variation among clinical and environmental isolates of b. pseudomallei. genes from four clinical and six environmental isolates were amplified and compared by rflp. the clinical isolates were indistinguishable, but variation was detected among some of the environmental isolates. sequence analysis of flagellin gene amplified products d ... | 1998 | 9877189 |
| the phn genes of burkholderia sp. strain rp007 constitute a divergent gene cluster for polycyclic aromatic hydrocarbon catabolism. | cloning and molecular ecological studies have underestimated the diversity of polycyclic aromatic hydrocarbon (pah) catabolic genes by emphasizing classical nah-like (nah, ndo, pah, and dox) sequences. here we report the description of a divergent set of pah catabolic genes, the phn genes, which although isofunctional to the classical nah-like genes, show very low homology. this phn locus, which contains nine open reading frames (orfs), was isolated on an 11.5-kb hindiii fragment from phenanthre ... | 1999 | 9882667 |
| burkholderia pseudomallei: the unbeatable foe? | although melioidosis has been recognized in thailand for many years and considerable progress in term of diagnosis and treatment was achieved, b. pseudomallei is still "the unbeatable foe", for several reasons as outlined here: under-recognition, high case-fatality rate, unacceptable relapse rate and a "time-bomb" for sero-positive patients. melioidosis is largely restricted to certain geographical areas. in thailand, it had long been considered a rare disease in thailand until ten cases with cu ... | 1998 | 9886137 |
| antigenic differences between clinical and environmental isolates of burkholderia pseudomallei. | burkholderia pseudomallei is a free-living organism that causes the potentially lethal tropical infection melioidosis. the disease is endemic in many parts of eastern asia and northern australia. the presence of two distinct biotypes in soil can be reliably distinguished by their ability to assimilate l-arabinose. whereas some soil isolates could utilize this substrate (ara+), the remaining soil isolates and all clinical isolates tested so far could not (ara-). only the ara- isolates were virule ... | 1998 | 9886145 |
| a melanin pigment purified from an epidemic strain of burkholderia cepacia attenuates monocyte respiratory burst activity by scavenging superoxide anion. | the acquisition of burkholderia cepacia in some cystic fibrosis patients is associated with symptoms of acute pulmonary inflammation that may be life threatening. the ability of lipopolysaccharide (lps) from b. cepacia to prime a monocyte cell line for enhanced superoxide anion generation was investigated and compared with the priming activities of lpss from pseudomonas aeruginosa, stenotrophomonas maltophilia, and escherichia coli. the human monocyte cell line monomac-6 (mm6) was primed overnig ... | 1999 | 9916107 |
| quorum sensing in burkholderia cepacia: identification of the luxri homologs cepri. | burkholderia cepacia has emerged as an important pathogen in patients with cystic fibrosis. many gram-negative pathogens regulate the production of extracellular virulence factors by a cell density-dependent mechanism termed quorum sensing, which involves production of diffusible n-acylated homoserine lactone signal molecules, called autoinducers. transposon insertion mutants of b. cepacia k56-2 which hyperproduced siderophores on chrome azurol s agar were identified. one mutant, k56-r2, contain ... | 1999 | 9922236 |
| biochemical and genetic evidence for meta-ring cleavage of 2,4, 5-trihydroxytoluene in burkholderia sp. strain dnt. | 2,4,5-trihydroxytoluene (tht) oxygenase from burkholderia sp. strain dnt catalyzes the conversion of tht to an unstable ring fission product. biochemical and genetic studies of tht oxygenase were undertaken to elucidate the mechanism of the ring fission reaction. the tht oxygenase gene (dntd) was previously localized to the 1.2-kb dna insert subcloned in the recombinant plasmid designated pjs76 (w. c. suen and j. c. spain, j. bacteriol. 175:1831-1837, 1993). analysis of the deduced amino acid se ... | 1999 | 9922262 |
| neonatal meningitis and septicaemia caused by burkholderia pseudomallei. | we report a case of meningitis and one of fatal septicaemia in neonates due to burkholderia pseudomallei and review the literature on neonatal melioidosis. pneumonia was the primary presentation and was complicated by shock in the latter case. the epidemiological findings suggest that the cases reported from malaysia were community-acquired in contrast with those from the usa and thailand. | 1998 | 9924578 |
| comparative in vitro activities of meropenem, imipenem, temocillin, piperacillin, and ceftazidime in combination with tobramycin, rifampin, or ciprofloxacin against burkholderia cepacia isolates from patients with cystic fibrosis. | we evaluated the activities of meropenem, imipenem, temocillin, piperacillin, and ceftazidime by determination of the mics for 66 genotypically characterized burkholderia cepacia isolates obtained from the sputum of cystic fibrosis patients. in vitro synergy assays, as performed by the time-kill methodology, of two- and three-drug combinations of the beta-lactams with tobramycin, rifampin, and/or ciprofloxacin were also performed with 10 strains susceptible, intermediate, or resistant to fluoroq ... | 1999 | 9925508 |
| inactivation of toluene 2-monooxygenase in burkholderia cepacia g4 by alkynes. | high concentrations of acetylene (10 to 50% [vol/vol] gas phase) were required to inhibit the growth of burkholderia cepacia g4 on toluene, while 1% (vol/vol) (gas phase) propyne or 1-butyne completely inhibited growth. low concentrations of longer-chain alkynes (c5 to c10) were also effective inhibitors of toluene-dependent growth, and 2- and 3-alkynes were more potent inhibitors than their 1-alkyne counterparts. exposure of toluene-grown b. cepacia g4 to alkynes resulted in the irreversible lo ... | 1999 | 9925593 |
| alterations in adhesion, transport, and membrane characteristics in an adhesion-deficient pseudomonad. | a stable adhesion-deficient mutant of burkholderia cepacia g4, a soil pseudomonad, was selected in a sand column assay. this mutant (env435) was compared to the wild-type strain by examining the adhesion of the organisms to silica sand and their transport through two aquifer sediments that differed in their sand, silt, and clay contents. we compared the longitudinal transport of the wild type and the adhesion mutant to the transport of a conservative chloride tracer in 25-cm-long glass columns. ... | 1999 | 9925613 |
| high-level formation of active pseudomonas cepacia lipase after heterologous expression of the encoding gene and its modified chaperone in escherichia coli and rapid in vitro refolding. | the lipase from pseudomonas cepacia atcc 21808 (recently reclassified as burkholderia cepacia) is widely used by organic chemists for enantioselective synthesis and is manufactured from recombinant p. cepacia harboring on a plasmid the clustered genes for lipase and its chaperone. high levels of expression of inactive lipase (40%) in escherichia coli were achieved with pcytexp1 under the control of the strong, temperature-inducible lambdaprl promoter. however, no overexpression of the lipase cha ... | 1999 | 9925617 |
| new assay for rhizobitoxine based on inhibition of 1-aminocyclopropane-1-carboxylate synthase. | rhizobitoxine is synthesized by the legume symbiont bradyrhizobium elkanii and the plant pathogen burkholderia andropogonis. rhizobitoxine competitively inhibited 1-aminocyclopropane-1-carboxylate (acc) synthase ble-acs2 from the tomato, a key enzyme in the pathway of ethylene biosynthesis. based on this inhibition of acc synthase, we have developed a new assay for rhizobitoxine. | 1999 | 9925628 |
| from the centers for disease control and prevention. nosocomial burkholderia cepacia infection and colonization associated with intrinsically contaminated mouthwash--arizona, 1998. | | 1999 | 9929074 |
| in vitro activity of meropenem against common pathogenic bacteria isolated in taiwan. | the in vitro antimicrobial activity of meropenem, in comparison with nine other antimicrobial agents, against 12 different common pathogenic bacteria were evaluated to know the susceptibility of common bacteria to meropenem in taiwan. meropenem was active against most gram-positive, gram-negative, and anaerobic bacteria, including methicillin-sensitive staphylococcus aureus, streptococcus pyogenes, enterococcus faecalis, escherichia coli, klebsiella pneumoniae, enterobacter cloacae, pseudomonas ... | 1998 | 9934544 |
| purification and characterization of tert-butyl ester-hydrolyzing lipase from burkholderia sp. yy62. | an intracellular novel lipase which can hydrolyze t-butyl octanoate (tbo) was purified to homogeneity from crude cell-free extracts of burkholderia (formerly pseudomonas) sp. yy62 with 9% overall yield. seventy-four-fold purification was achieved by ammonium-sulfate precipitation, three consecutive open-column chromatographies (deae anion-exchange, sepharose cl-6b gel-filtration, and the second deae anion-exchange columns), and two hplcs (tsk g2000swxl gel-filtration and phenyl 5pw hydrophobic i ... | 1998 | 9972257 |
| impact of microbiology practice on cumulative prevalence of respiratory tract bacteria in patients with cystic fibrosis. | investigators participating in the epidemiologic study of cystic fibrosis project began to collect microbiological, pulmonary, and nutritional data on cystic fibrosis (cf) patients at 180 north american sites in 1994. part of this study was a survey undertaken in august 1995 to determine microbiology laboratory practices with regard to pulmonary specimens from cf patients. the survey included a section on test ordering, completed by a site clinician, and a section on test performance and reporti ... | 1999 | 9986845 |
| [isolation and primary characteristics of a pseudomonas (burkholderia) pseudomallei plasmid]. | plasmid screening of reference pseudomonas pseudomallei strains isolated from patients and animals revealed cryptic plasmids with different molecular weights in 30 strains. plasmids were investigated by restriction analysis and dna-dna hybridization. cryptic plasmids were denoted as ppm1, pcm2, pcm3, and pcm4. the presence of plasmids in melioidosis agent permits their use for intraspecies typing of strains and for genetic studies. | 1998 | 9987750 |
| attachment of burkholderia pseudomallei to pharyngeal epithelial cells: a highly pathogenic bacteria with low attachment ability. | respiratory infections are initiated by the attachment of bacteria to pharyngeal epithelial cells. we studied the attachment of burkholderia pseudomallei to pharyngeal epithelial cells. after one, two, three, and four washes, there were 22.6+/-8.9, 15.7+/-7.0, 6.8+/-3.1, and 4.6+/-1.1 (mean+/-sd) attached bacteria/cell, respectively. if the bacterial concentration was maintained at 1 x 10(8) colony-forming units (cfu)/ml and three washes were done, at concentrations of 2.5 x 10(4), 5 x 10(4), an ... | 1999 | 9988329 |
| the type ii o-antigenic polysaccharide moiety of burkholderia pseudomallei lipopolysaccharide is required for serum resistance and virulence. | melioidosis, an infection caused by the gram-negative bacterial pathogen burkholderia pseudomallei, is endemic in south-east asia and northern australia. acute septicaemic melioidosis is a major cause of morbidity and mortality, especially in north-east thailand. b. pseudomallei is highly resistant to the bactericidal activity of normal human serum (nhs), and we have found that b. pseudomallei 1026b multiplies in 10-30% nhs. we developed a simple screen for the identification of serum-sensitive ... | 1998 | 9988483 |
| lipopolysaccharide (lps) from burkholderia cepacia is more active than lps from pseudomonas aeruginosa and stenotrophomonas maltophilia in stimulating tumor necrosis factor alpha from human monocytes. | whole cells and lipopolysaccharides (lpss) extracted from burkholderia cepacia, pseudomonas aeruginosa, stenotrophomonas maltophilia, and escherichia coli were compared in their ability to stimulate tumor necrosis factor alpha (tnf-alpha) from the human monocyte cell line monomac-6. b. cepacia lps, on a weight-for-weight basis, was found to have tnf-alpha-inducing activity similar to that of lps from e. coli, which was approximately four- and eightfold greater than the activity of lpss from p. a ... | 1999 | 10024601 |
| burkholderia cocovenenans (van damme et al. 1960) gillis et al. 1995 and burkholderia vandii urakami et al. 1994 are junior synonyms of burkholderia gladioli (severini 1913) yabuuchi et al. 1993 and burkholderia plantarii (azegami et al. 1987) urakami et al. 1994, respectively. | reference strains of burkholderia cocovenenans and burkholderia vandii were compared with strains of other burkholderia species using sds-page of whole-cell proteins, dna-dna hybridization and extensive biochemical characterization. burkholderia gladioli and b. cocovenenans were indistinguishable in the chemotaxonomic and biochemical analyses. burkholderia plantarii and b. vandii had indistinguishable whole-cell protein patterns but the b. vandii type strain differed from b. plantarii strains in ... | 1999 | 10028245 |
| burkholderia cepacia infections in cystic fibrosis. | | 1999 | 10048690 |
| efflux-mediated aminoglycoside and macrolide resistance in burkholderia pseudomallei. | burkholderia pseudomallei, the causative agent of melioidosis, is intrinsically resistant to a wide range of antimicrobial agents including beta-lactams, aminoglycosides, macrolides, and polymyxins. we used tn5-ot182 to mutagenize b. pseudomallei to identify the genes involved in aminoglycoside resistance. we report here on the identification of amrab-opra, a multidrug efflux system in b. pseudomallei which is specific for both aminoglycoside and macrolide antibiotics. we isolated two transposon ... | 1999 | 10049252 |
| lack of throat colonization with burkholderia cepacia among cystic fibrosis healthcare workers. | | 1999 | 10064207 |
| a nonhemolytic phospholipase c from burkholderia cepacia. | burkholderia cepacia is an opportunistic pathogen that causes serious pulmonary infections in cystic fibrosis patients. although several potential virulence factors-a protease, lipase, and two phospholipases c (one hemolytic and one nonhemolytic)-have been identified, only two, the protease and the lipase, have been described in detail. the goal of this study was to purify and characterize a nonhemolytic phospholipase c secreted by b. cepacia strain pc224c. the enzyme was concentrated from cultu ... | 1999 | 10069860 |
| effect of urokinase on the extracellular virulence properties of pseudomonas aeruginosa and burkholderia cepacia. | the influence of urokinase and oxygen availability on growth, siderophore, protease and lipase production in burkholderia cepacia and non-mucoid (pa01) and mucoid (pawh) strains of pseudomonas aeruginosa was assessed for cells grown in batch culture under iron-restriction. siderophore production decreased with increasing concentration of urokinase in b. cepacia independent of oxygen availability but decreased in both strains of p. aeruginosa only under oxygen-depleted conditions. protease activi ... | 1999 | 10071863 |
| comparison of isolation media for recovery of burkholderia cepacia complex from respiratory secretions of patients with cystic fibrosis. | burkholderia cepacia selective agar (bcsa) has previously been devised for isolation of b. cepacia from respiratory secretions of patients with cystic fibrosis and tested under research laboratory conditions. here we describe a study in which bcsa, oxidation-fermentation polymyxin bacitracin lactose agar (ofpbl), and pseudomonas cepacia agar (pca) were compared in routine culture procedures for the ability to grow b. cepacia and inhibit other organisms. three hundred twenty-eight specimens from ... | 1999 | 10074517 |
| identification and characterization of is2404 and is2606: two distinct repeated sequences for detection of mycobacterium ulcerans by pcr. | molecular analysis of mycobacterium ulcerans has revealed two new insertion sequences (iss), is2404 and is2606. is2404 was identified by complete sequencing of a previously described repetitive dna segment from m. ulcerans. this element is 1,274 bp long, contains 12-bp inverted repeats and a single open reading frame (orf) potentially encoding a protein of 327 amino acids (aa), and apparently generates 7-bp direct repeats upon transposition. amino acid similarity was found between the putative t ... | 1999 | 10074520 |
| melioidosis. | melioidosis is an infection with the gram-negative bacillus burkholdria pseudomallei, previous known as pseudomonas pseudomallei. we present a case report of a man with non-hodgkin's lymphoma, who after visiting indonesia presented himself with a urosepsis, with positive cultures to b. pseudomallei. melioidosis is endemic in areas of southeast asia and the northern part of australia. infection with the b. pseudomallei has a high mortality rate. diagnosis can be made on the basis of cultures, the ... | 1999 | 10079683 |
| all in the family: structural and evolutionary relationships among three modular proteins with diverse functions and variable assembly. | the crystal structures of three proteins of diverse function and low sequence similarity were analyzed to evaluate structural and evolutionary relationships. the proteins include a bacterial bleomycin resistance protein, a bacterial extradiol dioxygenase, and human glyoxalase i. structural comparisons, as well as phylogenetic analyses, strongly indicate that the modern family of proteins represented by these structures arose through a rich evolutionary history that includes multiple gene duplica ... | 1998 | 10082363 |
| timing of referral for lung transplantation for cystic fibrosis: overemphasis on fev1 may adversely affect overall survival. | (1) report our experience with referral for lung transplantation. (2) review survival in cvstic fibrosis (cf) patients without lung transplantation after fev1 remains < 30% predicted for 1 years. | 1999 | 10084492 |
| [melioidosis]. | | 1999 | 10088387 |
| in vitro activity of ciprofloxacin, levofloxacin, and trovafloxacin, alone and in combination with beta-lactams, against clinical isolates of pseudomonas aeruginosa, stenotrophomonas maltophilia, and burkholderia cepacia. | we tested three fluoroquinolones (ciprofloxacin, levofloxacin, and trovafloxacin), each combined with each of four beta-lactams (cefoperazone, ceftriaxone, imipenem, and meropenem) for synergy against clinical isolates of nosocomial strains of pseudomonas aeruginosa, stenotrophomonas maltophilia, and burkholderia cepacia. the ciprofloxacin-beta-lactam combinations showed synergy against none or only a small fraction (7 to 10%) of the p. aeruginosa and b. cepacia isolates. ciprofloxacin-cefoperaz ... | 1999 | 10091030 |
| structural analysis of a novel putative capsular polysaccharide from pseudomonas (burkholderia) caryophylli strain 2151. | a novel putative capsular polysaccharide consisting of d-glcp and d-fruf in the molar ratio of 1:1 was isolated as minor constituent from the lipopolysaccharide (lps) fraction of pseudomonas (burkholderia) caryophylli. its structure was determined, using mainly one- and two-dimensional nmr spectroscopy, as: -->6)-alpha-d-glcp-(1-->1)-beta-d-fruf-(2-->. | 1999 | 10092878 |
| effects of burkholderia pseudomallei and other burkholderia species on eukaryotic cells in tissue culture. | burkholderia pseudomallei causes melioidosis, a serious and often fatal bacterial infection. b. pseudomallei can behave as a facultatively intracellular organism and this ability may be important in the pathogenesis of both acute and chronic infection. the uptake of b. pseudomallei and other burkholderia spp. by cells in tissue culture was examined by electron microscopy. b. pseudomallei can invade cultured cell lines including phagocytic lines such as raw264, j774 and u937, and non-phagocytic l ... | 1998 | 10093229 |
| structural elucidation of a novel exopolysaccharide produced by a mucoid clinical isolate of burkholderia cepacia. characterization of a trisubstituted glucuronic acid residue in a heptasaccharide repeating unit. | the structure of the exopolysaccharide (eps) produced by a clinical isolate of burkholderia cepacia isolated from a patient with fibrocystic lung disease has been investigated. by means of methylation analyses, carboxyl reduction, partial depolymerization by fuming hcl and chemical degradations such as smith degradation, lithiumethylenediamine degradation and beta-elimination, supported by gc/ms and nmr spectroscopic analyses, the repeat unit of the eps has been identified and was shown to corre ... | 1999 | 10095771 |
| isolation and characterisation of a new antagonistic burkholderia strain from the rhizosphere of healthy tomato plants. | a new burkholderia strain (pvfi5a) which exhibits antagonism towards many bacterial and fungal plant pathogens has been partially characterised. this strain was isolated from the rhizosphere of tomato plants and was referred to the burkholderia cepacia complex on the basis of cultural, morphological and biochemical characteristics, including determination of the 16s ribosomal dna sequence and fatty acid profile. strain pvfi5a is a gram-negative, aerobic bacterium, oxidase- and catalase-positive, ... | 1999 | 10096133 |
| colonization and clearance of environmental microbial agents upon intranasal exposure of strain c3h/hej mice. | environmental dissemination of biotechnology agents is becoming a common practice. most applications use historically innocuous species; however, potential health effects of individual products are not scrutinized unless they contain genetically engineered microorganisms. in order to investigate possible health concerns, four surrogate microbial agents were studied in vivo. male c3h/hej (endotoxin-resistant) mice were administered intranasally (i.n.) with approximately 10(7) pseudomonas aureofac ... | 1999 | 10096364 |
| multiresistant non-fermentative gram-negative bacteria in cystic fibrosis patients: the results of an italian multicenter study. italian group for cystic fibrosis microbiology. | we carried out an epidemiological study on 2717 patients seen on a regular basis at italian cystic fibrosis centers with the aim of defining the prevalence of multiresistant gram-negative strains in italy. we found 272 multiresistant strains out of 1560 gram-negative strains isolated in 1012 italian cystic fibrosis patients. from our results in italian cystic fibrosis patients we may conclude: (1) the beta-lactam antibiotics are moderately active; ceftazidime is the most efficacious even if 59.9 ... | 1999 | 10099000 |
| activity and stability of a recombinant plasmid-borne tce degradative pathway in suspended cultures. | the retention and expression of the plasmid-borne, tce degradative toluene-ortho-monooxygenase (tom) pathway in suspended continuous cultures of transconjugant burkholderia cepacia 17616 (tom31c) were studied. acetate growth and tce degradation kinetics for the transconjugant host are described and utilized in a plasmid loss model. plasmid maintenance did not have a significant effect on the growth rate of the transconjugant. both plasmid-bearing and plasmid-free strains followed andrews inhibit ... | 1998 | 10099205 |
| modeling trichloroethylene degradation by a recombinant pseudomonad expressing toluene ortho-monooxygenase in a fixed-film bioreactor | burkholderia cepacia pr123(tom23c), expressing constitutively the tce-degrading enzyme toluene ortho-monooxygenase (tom), was immobilized on sirantrade mark glass beads in a biofilter for the degradation and mineralization of gas-phase trichloroethylene (tce). to interpret the experimental results, a mathematical model has been developed which includes axial dispersion, convection, film mass-transfer, and biodegradation coupled with deactivation of the tce-degrading enzyme. parameters used for n ... | 1998 | 10099312 |
| activity and stability of a recombinant plasmid-borne tce degradative pathway in biofilm cultures | the activity and stability of the tce degradative plasmid tom31c in the transconjugant host burkholderia cepacia 17616 was studied in selective and non-selective biofilm cultures. the activity of plasmid tom31c in biofilm cultures was measured by both tce degradative studies and the expression of the tom pathway. plasmid loss was measured using continuous flow, rotating annular biofilm reactors, and various analytical and microbiological techniques. the probability of plasmid loss in the biofilm ... | 1998 | 10099343 |
| optimization of pseudomonas cepacia lipase preparations for catalysis in organic solvents. | the activity of different lipase (from pseudomonas cepacia) forms, such as crude powder (crude pc), purified and lyophilized with peg (peg + pc), covalently linked to peg (peg-pc), cross-linked enzyme crystals (clec-pc), and immobilized in sol-gel-ak (sol-gel-ak-pc) was determined, at various water activities (aw), in carbon tetrachloride, benzene and 1,4-dioxane. the reaction of vinyl butyrate with 1-octanol was employed as a model and both transesterification (formation of 1-octyl butyrate) an ... | 1999 | 10099564 |
| [pulmonary melioidosis]. | melioidosis is most frequently encountered in pulmonary localization. melioidosis is an infectious disease caused by burkholderia pseudomallei first described by whitmore in 1912 in burma. b. pseudomallei is a gram negative rod belonging to the pseudomonadaceae family. soil and water are the natural reservoirs for the germ which is a specific pathogen for several mammal species. long endemic in southeast asia and several tropical zones, b. pseudomallei has recently been found in temperate zones, ... | 1998 | 10100350 |
| physiological and phylogenetic diversity of bacteria growing on resin acids. | resin acids are tricyclic diterpenes which are synthesized by trees and are a major cause of toxicity of pulp mill effluents. bacterial strains isolated from three different sources and which grow on resin acids were physiologically characterized. eleven strains, representating distinct groups, were further characterized physiologically and phylogenetically. the isolates had distinct specificities for use, as growth substrates, of the different resin acids tested. the isolates also used fatty ac ... | 1999 | 10188280 |
| effect of chronic intermittent administration of inhaled tobramycin on respiratory microbial flora in patients with cystic fibrosis. | pseudomonas aeruginosa endobronchial infection causes significant morbidity and mortality among cystic fibrosis patients. microbiology results from two multicenter, double-blind, placebo-controlled trials of inhaled tobramycin in cystic fibrosis were monitored for longitudinal changes in sputum microbial flora, antibiotic susceptibility, and selection of p. aeruginosa isolates with decreased tobramycin susceptibility. clinical response was examined to determine whether current susceptibility sta ... | 1999 | 10191222 |
| an epidemic of burkholderia cepacia transmitted between patients with and without cystic fibrosis. | burkholderia cepacia is an important pathogen in cystic fibrosis (cf) and an infrequent cause of nosocomial infection in non-cf patients. this report describes a large hospital outbreak that appeared to involve both patient groups, a previously unrecognized phenomenon. ribotype restriction fragment length polymorphism (rflp) profiles and pulsed-field gel electrophoresis-resolved macrochromosomal rflps were analyzed, a ribotype-based phylogenic tree was constructed, and case-control and cohort st ... | 1999 | 10191223 |
| genotypic and phenotypic relationships in burkholderia cepacia isolated from cystic fibrosis patients and the environment. | twenty-one strains of burkholderia cepacia isolated from the environment, and 21 clinical strains isolated principally from sputum of cystic fibrosis (cf) patients, were characterized genotypically by macrorestriction analysis (genome fingerprinting) and pcr ribotyping, and phenotypically by susceptibility to antibiotics and the ability to macerate onion tissue. the plasmid content of the strains was also investigated. environmental isolates showed a high degree of genetic variability, all strai ... | 1999 | 10196751 |
| rapid enumeration of physiologically active bacteria in purified water used in the pharmaceutical manufacturing process. | physiologically active bacteria in purified water used in the manufacturing process of pharmaceutical products were enumerated in situ. bacteria with growth potential were enumerated using the micro-colony technique and direct viable counting (dvc), followed by 24 h of incubation in 100-fold diluted scdb (soybean casein digest broth) at 30 degrees c. respiring and esterase-active bacteria were detected by fluorescent staining with 5-cyano-2,3-ditolyl tetrazolium chloride (ctc) and 6-carboxyfluor ... | 1999 | 10196754 |
| discrimination of burkholderia multivorans and burkholderia vietnamiensis from burkholderia cepacia genomovars i, iii, and iv by pcr. | we present a pcr procedure for identification of burkholderia cepacia, burkholderia multivorans, and burkholderia vietnamiensis. 16s and 23s ribosomal dnas (rdnas) of b. multivorans and b. vietnamiensis were sequenced and aligned with published sequences for definition of species-specific 18-mer oligonucleotide primers. specific antisense 16s rdna primers (for b. cepacia, 5'-agc act ccc rcc tct cag-3'; for b. multivorans, 5'-agc act ccc gaa tct ctt-3') and 23s rdna primers (for b. vietnamiensis, ... | 1999 | 10203482 |
| pcr-rflp analysis of the flagellin sequences for identification of burkholderia pseudomallei and burkholderia cepacia from clinical isolates. | the flagellin genes of four burkholderia pseudomallei and two burkholderia cepacia clinical isolates were studied by a polymerase chain reaction (pcr)-based isolation method using the same pair of primers. the pcr-amplification products of the isolates showed a single band of about 1.1 kb, which is similar to a type ii b. cepacia flagellin reported previously. in order to distinguish these two species based on the flagellin pcr-amplified products, pst i and xho i restriction endonuclease analysi ... | 1999 | 10208800 |
| equine glanders in turkey. | in the course of an epidemiological study of glanders on a number of turkish islands in the sea of marmara, 1128 horses were examined by using the intracutaneous mallein test. thirty-five (3-1 per cent) developed an increase in rectal temperature and a swelling at the point of injection. ten of these horses were killed and glanders was confirmed in five cases by the presence of lesions and by the immunohistological demonstration of the causative agent, burkholderia mallei. clinical and pathologi ... | 1999 | 10209817 |
| expression and functional analysis of an n-truncated nifa protein of herbaspirillum seropedicae. | in herbaspirillum seropedicae, an endophytic diazotroph, nif gene expression is under the control of the transcriptional activator nifa. we have over-expressed and purified a protein containing the central and c-terminal domains of the h. seropedicae nifa protein, n-truncated nifa, fused to a his-tag sequence. this fusion protein was found to be partially soluble and was purified by affinity chromatography. band shift and footprinting assays showed that the n-truncated nifa protein was able to b ... | 1999 | 10214962 |
| hydroxylation reaction catalyzed by the burkholderia cepacia ac1100 bacterial strain. involvement of the chlorophenol-4-monooxygenase. | the burkholderia cepacia ac1100 strain, known to degrade the herbicide, 2,4,5-trichlorophenoxyacetic acid (2,4,5-t), is able to metabolize 4-hydroxyarylaldehyde, not only into the corresponding acid, but also into a new hydroquinone, 2,5-dihydroxyarylaldehyde. when incubated with resting ac1100 cells or cell-free extracts, syringaldehyde and 3,5-dimethoxy-4-hydroxybenzaldehyde were converted into such metabolites, identified by comparison of their mass and 1h-nmr spectra with those of authentic ... | 1999 | 10215866 |
| cloning and sequence analysis of the lipase and lipase chaperone-encoding genes from acinetobacter calcoaceticus rag-1, and redefinition of a proteobacterial lipase family and an analogous lipase chaperone family. | the genes encoding the lipase (lipa) and lipase chaperone (lipb) from acinetobacter calcoaceticus rag-1 were cloned and sequenced. the genes were isolated from a genomic dna library by complementation of a lipase-deficient transposon mutant of the same strain. transposon insertion in this mutant and three others was mapped to a single site in the chaperone gene. the deduced amino acid (aa) sequences for the lipase and its chaperone were found to encode mature proteins of 313 aa (32.5kda) and 347 ... | 1999 | 10216267 |
| the many facets of melioidosis. | | 1999 | 10217823 |
| commercial use of burkholderia cepacia. | | 1999 | 10221892 |
| activity of abundant antimicrobials of the human airway. | human airways produce several antimicrobial factors; the most abundant are lysozyme and lactoferrin. despite their likely importance in preventing infection, and their possible key role in the pathogenesis of cystic fibrosis (cf), we know little about their antibacterial activity in the context of the cf airway. we found that abundant airway antimicrobial factors kill common cf pathogens, although burkholderia was relatively resistant. to study the antibacterial activity, we developed a rapid, s ... | 1999 | 10226057 |
| pyogenic lung infections. | pyogenic lung infections still occur despite the availability of effective antibiotics for the treatment of patients with acute bacterial pneumonia. our understanding of the pathogenesis and management of these conditions has steadily improved over the past few decades, although some areas remain obscure. the effect of hiv infection on the incidence of pyogenic lung infections remains largely unknown, and large studies are required to evaluate this. burkholderia (formerly pseudomonas) cepacia st ... | 1999 | 10228739 |
| nitric oxide-induced potentiation of the killing of burkholderia cepacia by reactive oxygen species: implications for cystic fibrosis. | burkholderia (formerly pseudomonas) cepacia has emerged as an important pulmonary pathogen in cystic fibrosis, and survives within the lung despite a vigorous neutrophil-dominated immune response. nitric oxide (no) contributes to the antimicrobial activity of reactive oxygen species in the normal lung, but recent evidence suggests that inducible no synthase is not expressed in the airway epithelial cells of cystic fibrosis (cf) patients. this may explain the failure of the neutrophil response to ... | 1999 | 10229538 |
| burkholderia caribensis sp. nov., an exopolysaccharide-producing bacterium isolated from vertisol microaggregates in martinique. | twenty-one exopolysaccharide-producing strains were isolated from the 5-20 microns fraction of a vertisol in the south-east of the island of martinique in the french west indies. although these strains were phenotypically identified as burkholderia cepacia or as burkholderia glathei using biolog microplates, they did not cluster genotypically by amplified rdna restriction analysis (ardra) with any described burkholderia species. a phylogenetic analysis revealed that the rrs (16s rdna) sequences ... | 1999 | 10319504 |
| role of quinolinate phosphoribosyl transferase in degradation of phthalate by burkholderia cepacia dbo1. | two distinct regions of dna encode the enzymes needed for phthalate degradation by burkholderia cepacia dbo1. a gene coding for an enzyme (quinolinate phosphoribosyl transferase) involved in the biosynthesis of nad+ was identified between these two regions by sequence analysis and functional assays. southern hybridization experiments indicate that dbo1 and other phthalate-degrading b. cepacia strains have two dissimilar genes for this enzyme, while non-phthalate-degrading b. cepacia strains have ... | 1999 | 10322007 |
| phylogenetic analysis of ara+ and ara- burkholderia pseudomallei isolates and development of a multiplex pcr procedure for rapid discrimination between the two biotypes. | a burkholderia pseudomallei-like organism has recently been identified among some soil isolates of b. pseudomallei in an area with endemic melioidosis. this organism is almost identical to b. pseudomallei in terms of morphological and biochemical profiles, except that it differs in ability to assimilate l-arabinose. these ara+ isolates are also less virulent than the ara- isolates in animal models. in addition, clinical isolates of b. pseudomallei available to date are almost exclusively ara-. t ... | 1999 | 10325345 |
| characterization of a murine model of melioidosis: comparison of different strains of mice. | melioidosis is an infectious disease caused by the saprophytic gram-negative rod burkholderia pseudomallei. the aim of this study was to establish and characterize a murine model of melioidosis to provide a basis for further investigations on the pathogenesis of the disease. after intravenous infection with b. pseudomallei, c57bl/6 mice were found to be significantly more resistant than balb/c mice. there was a marked organotropism of b. pseudomallei for the spleen and liver in both strains of m ... | 1999 | 10338496 |
| burkholderia cepacia bronchiectasis. | | 1999 | 10343643 |