| [the immunogenicity and heterogeneity of pseudomonas pseudomallei surface antigen 8]. | surface antigen 8, one of pathogenicity factors of p.pseudomallei and having an antiphagocytic action, contains glycoprotein with a mol. wt. of 200 kd and proteins with mol wt. of 25, 30 and 34 kd. according to its chemical structure, the carbohydrate part of antigen 8 is the homogeneous polymer of 6-d-d-mannoheptose, and its protein component is a collection of monomer proteins with mol. wt. of 12-120 kd. the consecutive fractionation of antigen 8 by gel and ion exchange chromatography has made ... | 1996 | 9027183 |
| identifying treatments that halt progression of pulmonary disease in cystic fibrosis. | rapid progress in cystic fibrosis research affords the possibility of halting the progress of the lung disease. we used data from 215 patients who had sputum cultures negative for burkholderia cepacia, at least one outpatient pulmonary function test during 1990, and at least one test a year later to estimate the number of subjects and study duration required to demonstrate that a hypothetical treatment reduces the rate of decline of forced expiratory volume in 1 s (fev1) to zero. mean rate of de ... | 1997 | 9029632 |
| multifactorial pathogenic mechanisms of burkholderia pseudomallei as suggested from comparison with burkholderia cepacia. | with the purpose to elucidate the pathogenesis of disease due to burkholderia pseudomallei some biological and biochemical properties of this species were studied in comparison with b. cepacia, since the difference in the level of virulence between the two species is remarkable despite of their toxonomic closeness. b. pseudomallei was distinct from b. cepacia in the capability to grow under anaerobic conditions, with positive nitrate respiration, excretion of high-molecular polysaccharides into ... | 1996 | 9031412 |
| the crystal structure of a triacylglycerol lipase from pseudomonas cepacia reveals a highly open conformation in the absence of a bound inhibitor. | . lipases, a family of enzymes which catalyze the hydrolysis of triglycerides, are widely distributed in many organisms. true lipases are distinguished from esterases by the characteristic interfacial activation they exhibit at an oil-water interface. lipases are one of the most frequently used biocatalysts for organic reactions performed under mild conditions. their biotechnological applications include food and oil processing and the preparation of chiral intermediates for the synthesis of ena ... | 1997 | 9032073 |
| the open conformation of a pseudomonas lipase. | . the interfacial activation of lipases results primarily from conformational changes in the enzymes which expose the active site and provide a hydrophobic surface for interaction with the lipid substrate. comparison of the crystallization conditions used and the structures observed for a variety of lipases suggests that the enzyme conformation is dependent on solution conditions. pseudomonas cepacia lipase (pcl) was crystallized in conditions from which the open, active conformation of the enzy ... | 1997 | 9032074 |
| identification of burkholderia cepacia isolates from patients with cystic fibrosis and use of a simple new selective medium. | we evaluated 819 isolates referred to us as "burkholderia cepacia" from cystic fibrosis (cf) clinics and research laboratories from five countries; 28 (3.4%) were not b. cepacia. a further 12 (1.5%) organisms appeared to be other burkholderia species, but identification could not be confirmed by conventional means. the most prevalently misidentified organisms were stenotrophomonas maltophilia, alcaligenes xylosoxidans, and comamonas acidovorans. many of these organisms grew on oxidation-fermenta ... | 1997 | 9041399 |
| [microbiological parameters of clinical interest in pulmonary infection in cystic fibrosis]. | a 5-year study in patients with cystic fibrosis was carried out in order to gain a better understanding of the microbiological factors influencing clinical status and evolution. | 1996 | 9044640 |
| cloning and expression of the major porin protein gene opcp of burkholderia (formerly pseudomonas) cepacia in escherichia coli. | the outer membrane protein opcp1 of burkholderia (formerly pseudomonas) cepacia is one of the subunits forming the porin oligomer opcpo by non-covalent association. opcp1 was cleaved with lysyl endopeptidase and the n-terminal amino acid (aa) sequences of the polypeptide fragments were determined. based on the sequence information, we cloned the opcp gene on a 10-kb ecori dna fragment of the b. cepacia atcc25416 chromosome. nucleotide (nt) sequencing revealed a 1086-bp open reading frame (orf), ... | 1997 | 9047353 |
| probing of pseudomonas aeruginosa, pseudomonas aureofaciens, burkholderia (pseudomonas) cepacia, pseudomonas fluorescens, and pseudomonas putida with the ferripyochelin receptor a gene and the synthesis of pyochelin in pseudomonas aureofaciens, pseudomonas fluorescens, and pseudomonas putida. | the ferripyochelin receptor a (fpta) gene codes for the transport of the ferrisiderophore ferripyochelin in pseudomonas aeruginosa. a p. aeruginosa fpta internal fragment was used to probe chromosomal dna from p. aureofaciens, b. cepacia, p. fluorescens, p. putida, and five strains of p.aeruginosa. these bacteria all contained dna that hybridized to the fpta fragment. four of the five p. aeruginosa strains displayed marked and identical patterns, indicating a high degree of sequence similarities ... | 1997 | 9058547 |
| serum igg response to an outer membrane porin protein of burkholderia cepacia in patients with cystic fibrosis. | early and accurate diagnosis of burkholderia cepacia infection is important, particularly if segregation is to prevent patient-to-patient transmission. we have examined the serum response to a b. cepacia-specific 80-kda outer membrane protein. 21 patients colonised with b. cepacia and pseudomonas aeruginosa for 2-51 months (mean 11 months) were age- and sex-matched with 21 patients colonised with p. aeruginosa but not b. cepacia. the 80-kda protein was recovered by electroelution from outer memb ... | 1997 | 9061354 |
| lung infection with burkholderia gladioli in a child with cystic fibrosis: acute clinical and spirometric deterioration. | | 1997 | 9065952 |
| fatal human melioidosis in south-eastern queensland. | two simultaneous human cases of fatal melioidosis in temperate south-eastern queensland involved patients who had had pre-existing multisystem illnesses, had sustained cutaneous lesions before illness onset, and died from overwhelming sepsis. onset of disease was preceded by unseasonably heavy rainfall. these and other features of these cases suggest that the source of infection was local, in which case the endemicity of burkholderia pseudomallei in temperate regional australia may be broader th ... | 1997 | 9066549 |
| the laboratory diagnosis of melioidosis. | the recognition of unusual, but important, pathogens such as burkholderia pseudomallei is essential for the rapid implementation of appropriate antimicrobial therapy--delays can be fatal. melioidosis should be considered as a potential diagnosis for any patient with exposure to areas of endemicity, and thus laboratories should be aware of the differential features of the disease and the causative organism. isolation of b. pseudomallei is readily achieved using standard culture media such as bloo ... | 1996 | 9069100 |
| isolation from clinical sources of burkholderia cepacia possessing characteristics of burkholderia gladioli. | further characterization of 32 clinical isolates originally identified as burkholderia cepacia by biochemical and fatty acid profiling revealed the presence of 12 strains bearing partial resemblance to the closely related species burkholderia gladioli. these strains were highly resistant to a wide range of antibiotics including ticarcillin (with or without clavulanic acid), cefsulodin, imipenem, the aminoglycosides, colistin and fosfomycin. they typically produced a red-brown pigment and possess ... | 1997 | 9069537 |
| detection of burkholderia pseudomallei in blood samples using polymerase chain reaction. | a highly sensitive, specific, rapid and simple method to detect burkholderia pseudomallei in blood samples was developed. two 22-base oligonucleotide primers, based on sequences from a specific dna probe, were used for amplification of bacterial dna by the polymerase chain reaction (pcr). amplification with these primers yielded a 178-base pair product in 100 clinical isolates of b. pseudomallei. as little as 0.5 fg of b. pseudomallei dna was detectable by this method. experiments involving inoc ... | 1997 | 9076711 |
| mutagenesis of burkholderia pseudomallei with tn5-ot182: isolation of motility mutants and molecular characterization of the flagellin structural gene. | burkholderia pseudomallei is a human and animal pathogen in tropical regions, especially southeast asia and northern australia. currently little is known about the genetics and molecular biology of this organism. in this report, we describe the mutagenesis of b. pseudomallei with the transposon tn5-ot182. b. pseudomallei 1026b transposon mutants were obtained at a frequency of 4.6 x 10(-4) per initial donor cell, and the transposon inserted randomly into the chromosome. we used tn5-ot182 to iden ... | 1997 | 9079894 |
| [insertion mutations in pseudomonas pseudomallei genome induced by transposons tn10. tn9, and tn5]. | tn10 and tn9 from temperature-sensitive replicons rpi::tn10 rep is and rp1::tn9 rep ts and tn5 from the "suicidal" vector psup5011 may integrate in the p. pseudomallei chromosome. transposons induced auxotrophic mutations of different spectrum, depending on the strain, tn element, and defects in the genes responsible for the mobility sign, function of some extracellular elements (lecithinase and lipase), hemolytic activity, and antigen 8 synthesis, which are considered as the potential factors o ... | 1997 | 9082181 |
| [creation of genomic dna libraries for pseudomonas mallei and pseudomonas pseudomallei]. | the genomic libraries of p. mallei and p. pseudomallei species were constructed in escherichia coli. the chromosomal dna of p. pseudomallei c-141 strain has been cloned into the cosmid vector phc79 and the broad host range plasmid vector pes154. the chromosomal dna of p. mallei [symbol: see text]-5 strain has been cloned into the plasmid vector psup202. the recombinant clones of the genomic libraries were screened by the enzyme-linked immunoadsorbent assay (elisa) to detect the production of pse ... | 1997 | 9082182 |
| substituted amines synergistic with tobramycin against burkholderia cepacia in vitro. | antibiotic-resistant burkholderia cepacia is an important pathogen in cystic fibrosis. we have previously shown enhanced tobramycin susceptibility of most strains in the presence of the drug amiloride. others have shown similar findings in the presence of cationic agents with amine groups. to examine the relationship of substituted amine drug structure and synergy potential with tobramycin, the in vitro activity of 45 non-antibiotic substituted amine and related compounds in the presence and abs ... | 1997 | 9084918 |
| conservation of the multidrug resistance efflux gene oprm in pseudomonas aeruginosa. | an intragenic probe derived from the multidrug resistance gene oprm hybridized with genomic dna from all 20 serotypes of pseudomonas aeruginosa and from all 34 environmental and clinical isolates tested, indicating that the mexa-mexb-oprm multidrug efflux system is highly conserved in this organism. the oprm probe also hybridized with genomic dna from pseudomonas aureofaciens, pseudomonas chlororaphis, pseudomonas syringae, burkholderia pseudomallei, and pseudomonas putida, suggesting that efflu ... | 1997 | 9087505 |
| exercise testing and prognosis in adult cystic fibrosis. | the assessment of prognosis is an important issue in cystic fibrosis. the prognostic value of exercise testing in comparison with other predictors of mortality was examined. | 1997 | 9093351 |
| a fusion promoter created by a new insertion sequence, is1490, activates transcription of 2,4,5-trichlorophenoxyacetic acid catabolic genes in burkholderia cepacia ac1100. | transposition and transcriptional activation by insertion sequences in burkholderia cepacia ac1100 were investigated. two closely related new elements, is1413 and is1490, were identified and characterized. these elements are not highly related to other insertion sequences identified in ac1100 or other b. cepacia isolates. based on their structures and the sequences of the inverted terminal repeats and the putative transposase protein, the insertion elements (is elements) are similar to ist2 of t ... | 1997 | 9098071 |
| molecular cloning, characterization, and mutagenesis of a pel gene from pseudomonas syringae pv. lachyrmans encoding a member of the erwinia chrysanthemi pelade family of pectate lyases. | the pels gene from pseudomonas syringae pv. lachrymans 859 was cloned by heterologous expression in nonpectolytic p. syringae pv. syringae buvs1, using genomic dna libraries constructed with two novel broad-host-range cosmid vectors, pcpp34 and pcpp47. screening of p. syringae pv. syringae transconjugants for the ability to pit pectate media at ph 6.0 and 8.5 yielded several overlapping clones of the same dna region. ultrathin-layer isoelectric focusing gels, activity-stained with diagnostically ... | 1997 | 9100381 |
| nosocomial acquisition of burkholderia gladioli in patients with cystic fibrosis. | burkholderia gladioli has been reported as colonizing the airways of patients with cystic fibrosis (cf) but has not previously been associated with adverse outcome. we describe six patients with cf in whom the same strain of b. gladioli, on the basis of ribotyping and biochemical characteristics, was grown in their sputum. acquisition of this organism was followed by a fatal outcome in all six patients; one had a rapid decline in respiratory status and another developed fulminant b. gladioli bac ... | 1997 | 9105090 |
| enhanced host defense after gene transfer in the murine p47phox-deficient model of chronic granulomatous disease. | the p47phox-/- mouse exhibits a phenotype similar to that of human chronic granulomatous disease (cgd) and, thus, is an excellent model for the study of gene transfer technology. using the moloney murine leukemia virus-based retroviral vector mfg-s encoding the human form of p47phox, we performed ex vivo gene transfer into sca-1+ p47phox-/- marrow progenitor cells without conditioning of donors with 5-fluorouracil. transduced progenitors were transplanted into moderately irradiated (500 cgy), g- ... | 1997 | 9116268 |
| pcr ribotyping and endonuclease subtyping in the epidemiology of burkholderia cepacia infection. | because of conflicting data about hospital-based transmission of burkholderia (pseudomonas) cepacia, an important respiratory pathogen in cystic fibrosis (cf), we compared strains found in sputum, lung, or blood of 29 cf patients in our center from 1988 to 1994, studying the relationship between strain and hospital exposure of incident and that of prevalent cases. exposure was defined as a concurrent hospital stay between a prevalent and an incident case. b. cepacia strains were determined by po ... | 1997 | 9117036 |
| comparative in vitro activity of meropenem versus other extended-spectrum antimicrobials against randomly chosen and selected resistant clinical isolates tested in 26 north american centers. | the in vitro antibacterial activity of meropenem and up to nine other antimicrobials was compared in studies at 26 north american centers from 1989 to 1992 with use of standardized and controlled procedures for determining minimal inhibitory concentrations (mics) against 12,483 recent clinical isolates and additional drug-resistant strains. overall, carbapenems were the most active drugs. the antibacterial activity of meropenem was consistent against random isolates in all centers; however, incl ... | 1997 | 9126699 |
| neurological melioidosis (burkholderia pseudomallei) mimicking guillain-barré syndrome. | | 1997 | 9127663 |
| characterization of burkholderia pseudomallei and burkholderia pseudomallei-like strains. | previous reports in the literature suggest that burkholderia pseudomallei strains can be differentiated on the basis of animal virulence. twenty environmentally and clinically derived isolates of burkholderia pseudomallei were examined for the production of exoenzymes, morphological and biochemical phenotypes and virulence for syrian golden hamsters. the partial sequence of the 16s ribosomal rna [rrna] genes from a number of these strains was also determined. based upon these observations, it is ... | 1997 | 9129590 |
| absence of burkholderia cepacia from the respiratory tract of non-cystic fibrosis patients. | | 1997 | 9131333 |
| geographical distribution of pseudomonas pseudomallei in china. | 1,366 samples of soil and water from southern china coastal provinces were examined for pseudomonas pseudomallei. data showed that 58 samples were positive for this bacteria, which is primarily distributed in hainan province and the coastal region of mainland. this paper confirmed the environmental presence of p. pseudomallei in china and showed that the distribution of this pathogen has at least reached a latitude of 25.5 degrees north. | 1995 | 9139366 |
| burkholderia pseudomallei infection in the singapore armed forces from 1987 to 1994--an epidemiological review. | between 1987 and 1994, twenty-three cases of burkholderia pseudomallei infection (melioidosis) were diagnosed in persons serving in the singapore armed forces. there were four deaths resulting from complications of the infection. unlike the situation in the general population, where the affected are mainly the elderly with underlying illness, the majority of cases in the singapore armed forces were otherwise fit and healthy young servicemen. serological surveys have shown the prevalence of the i ... | 1997 | 9140571 |
| isolation of an acanthamoeba strain with intracellular burkholderia pickettii infection. | burkholderia pickettii is a facultative pathogen that has been isolated from patient sources and environmental sources including respiratory therapy solutions, deionized water and aqueous disinfectants. the organism has been associated with septicemia and respiratory tract infections. in our investigation, burkholderia pickettii (biovar 2) was for the first time isolated from acanthamoeba sp. (group ii), a free living amoeba species recovered from the wet area of a physiotherapy unit. pathogenic ... | 1997 | 9144916 |
| antibacterial activity of bms-180680, a new catechol-containing monobactam. | the in vitro activities of a new catechol-containing monobactam, bms-180680 (sq 84,100), were compared to those of aztreonam, ceftazidime, imipenem, piperacillin-tazobactam, ciprofloxacin, amikacin, and trimethoprim-sulfamethoxazole. bms-180680 was often the most active compound against many species of the family enterobacteriaceae, with mics at which 90% of the isolates were inhibited (mic90s) of < or = 0.5 microg/ml for escherichia coli, klebsiella spp., citrobacter diversus, enterobacter aero ... | 1997 | 9145861 |
| septicemia and suppuration in a vietnam veteran. | | 1997 | 9153149 |
| the effects of panresistant bacteria in cystic fibrosis patients on lung transplant outcome. | the number of cystic fibrosis (cf) patients undergoing lung transplant continues to rise as long term survival improves. one major contraindication to this potentially life-saving intervention is infection with multi-drug-resistant bacteria. we undertook this retrospective study in 66 transplanted patients over 6 yr to determine the influence of panresistant bacteria on transplant outcome. the in vitro antibiotic susceptibility pattern of respiratory tract bacteria obtained pre- and/or intraoper ... | 1997 | 9154879 |
| identification and characterization of a novel dna marker associated with epidemic burkholderia cepacia strains recovered from patients with cystic fibrosis. | burkholderia cepacia is a problematic pathogen that may spread among patients with cystic fibrosis (cf). one highly infectious cf strain that causes epidemics in both the united kingdom and eastern canada has been shown to possess both the cable pilin subunit gene (cbla) and a unique combination of insertion sequences. however, no genetic markers linking this strain type with other types epidemic at various centers have been identified. using a randomly amplified polymorphic dna (rapd) typing sc ... | 1997 | 9157133 |
| diagnostic value of an antibody enzyme-linked immunosorbent assay using affinity-purified antigen in an area endemic for melioidosis. | melioidosis, an infection caused by burkholderia pseudomallei, is endemic in southeast asia. the septicemic form of melioidosis is the leading cause of death from nonhospital-acquired septicemia in the northeastern part of thailand. a major factor that contributes to the high mortality is the delay in isolation and identification of the causative organism. the present study was undertaken to evaluate the use of enzyme-linked immunosorbent assays based on an immunoaffinity-purified antigen for de ... | 1997 | 9158051 |
| comparison of biotyping, ribotyping, and pulsed-field gel electrophoresis for investigation of a common-source outbreak of burkholderia pickettii bacteremia. | over a 3-month period, six immunocompromised patients developed one or more episodes of burkholderia pickettii bacteremia and/or catheter infection. vials of a commercially available, "sterile" saline for injection which had been used for flushing the patients' indwelling intravenous devices were implicated as the common source of the organisms. no further cases were diagnosed once the use of this saline was discontinued. twenty-six isolates, including 9 outbreak-related strains from case patien ... | 1997 | 9163452 |
| cloning, characterization, and sequence analysis of the clce gene encoding the maleylacetate reductase of pseudomonas sp. strain b13. | a 3,167-bp psti fragment of genomic dna from pseudomonas sp. strain b13 was cloned and sequenced. the gene clce consists of 1,059 nucleotides encoding a protein of 352 amino acids with a calculated mass of 37,769 da which showed maleylacetate reductase activity. the protein had significant sequence similarities with the polypeptides encoded by tcbf of pp51 (59.4% identical positions), tfdf of pjp4 (55.1%), and tfte of burkholderia cepacia ac1100 (53.1%). the function of tcbf as maleylacetate red ... | 1997 | 9171435 |
| four genes from pseudomonas fluorescens that encode the biosynthesis of pyrrolnitrin. | pyrrolnitrin is a secondary metabolite of pseudomonas and burkholderia sp. strains with strong antifungal activity. production of pyrrolnitrin has been correlated with the ability of some bacteria to control plant diseases caused by fungal pathogens, including the damping-off pathogen rhizoctonia solani. pseudomonas fluorescens bl915 has been reported to produce pyrrolnitrin and to be an effective biocontrol agent for this pathogen. we have isolated a 32-kb genomic dna fragment from this strain ... | 1997 | 9172332 |
| isolation and identification of burkholderia pseudomallei from soil using selective culture techniques and the polymerase chain reaction. | an environmental soil survey to detect burkholderia pseudomallei was performed during the dry and wet seasons in darwin, northern territory, australia. soil was sampled at regular intervals during a 15-month period at different depths from areas which were representative of the local, soil environment. selective culture techniques using ashdown's and galimand and dodin's methods and the polymerase chain reaction (pcr) using specific 16s rrna primers were used to detect and identify the organism ... | 1997 | 9172400 |
| structure of catechol 2,3-dioxygenase gene encoded in tom plasmid of pseudomonas cepacia g4. | the catechol 2,3-dioxygenase is an extradiol-type dioxygenase which cleaves the c-c bond at the meta position of catechol to form 2-hydroxymuconic semialdehyde. a catechol 2,3-dioxygenase gene (tomb) in the tom plasmid of p. cepacia g4 has been cloned and its nucleotide sequence was analyzed. the enzyme gene consisted of 945 base pairs with an atg initiation codon and a tga termination codon which can encode a polypeptide of molecular weight 35 kda containing 314 amino acid residues, and a putat ... | 1997 | 9175755 |
| two patients with recurrent melioidosis after prolonged antibiotic therapy. | melioidosis is a tropical infectious disease caused by burkholderia (pseudomonas) pseudomalleï. clinically manifest melioidosis occurs mostly in people with underlying disorders. melioidosis is a disease with protein manifestations and a high rate of relapse. two patients with infection due to burkholderia pseudomalleï after a visit to thailand are described. both patients presented with sepsis and appropriate therapy was initiated and continued for several months. despite such long-term antimic ... | 1997 | 9181661 |
| in-vitro activity of carbapenem antibiotics against beta-lactam susceptible and resistant strains of burkholderia pseudomallei. | four carbapenem antibiotics were tested for their in-vitro activities (mics, mbcs and time-kill studies) against burkholderia pseudomallei. the carbapenems were all more active than either ceftazidime or co-amoxiclav against strains of b. pseudomallei with a normal susceptibility pattern. biapenem was the most active antibiotic tested. all four carbapenems retained bactericidal activity against 24 strains of b. pseudomallei with reduced susceptibility to ceftazidime and/or co-amoxiclav. | 1996 | 9182118 |
| affinity and response of burkholderia pseudomallei and burkholderia cepacia to insulin. | the cells of burkholderia pseudomallei, b. cepacia and pseudomonas aeruginosa grown on agar plates were stained with fluorescently-labeled insulin. the former two species were stained positively indicating insulin binding but p. aeruginosa was not. insulin exposure reduced phospholipase c and acid phosphatase activities of b. pseudomallei but did not affect those enzymatic activities of b. cepacia in the employed experimental conditions. it is suggested that b. pseudomallei have insulin receptor ... | 1996 | 9185275 |
| evolution of cell-surface acid phosphatase of burkholderia pseudomallei. | acid phosphatase active fractions were obtained from cell-free extract, outermembrane fraction and culture filtrate of burkholderia pseudomallei by column chromatography with sepharose 6b and deae cellulose. the comparison of the elution patterns of protein, sugar and enzymatic activity among these three components suggested that the enzyme is a glycoprotein evolving from premature proteins through glycosylation and that the enzyme is translocated during glycosylation from the cytoplasm to the o ... | 1996 | 9185276 |
| phylogenetic and physiological comparisons of pah-degrading bacteria from geographically diverse soils. | the diversity of bacteria isolated from creosote- contaminated soils in the united states, norway, and germany was determined by comparing their ability to degrade polycyclic aromatic hydrocarbons (pahs), their phospholipid ester-linked fatty acid (gc-fame) profiles, sole carbon source utilization patterns (biolog assays), and 16s rrna sequences. bacteria were initially obtained by enrichment with phenanthrene and fluoranthene. many were capable of degrading a broad range of the pahs found in cr ... | 1997 | 9195008 |
| extradural hematoma following maxillary sinusitis. case illustration. | | 1997 | 9202282 |
| structure of a new 6-deoxy-alpha-d-talan from burkholderia (pseudomonas) plantarii strain dsm 6535, which is different from the o-chain of the lipopolysaccharide. | an o-acetylated homopolysaccharide of 6-deoxy-d-talose (6-deoxy-alpha-d-talan polymer) was isolated from burkholderia (pseudomonas) plantarii strain dsm 6535 by extraction with 2-propanol. the structure (1) of the trisaccharide repeating unit of the polysaccharide was established by studies of the intact and o-deacetylated polysaccharides using methanolysis, methylation analysis, 1h and 13c nmr spectroscopy, including 2d cosy, heteronuclear 13c, 1h cosy, 1d noe, and computer-assisted analysis of ... | 1997 | 9203339 |
| caesarean section in a parturient with respiratory failure caused by cystic fibrosis. | we describe a 27-year-old primigravida suffering from cystic fibrosis. her chest was colonised with burkholderia cepacia and she was in respiratory failure for which she required constant nasal intermittent positive pressure ventilation. in view of her rapid deterioration, caesarean section was performed under epidural anaesthesia at 25 weeks gestation. a live 790-g boy was delivered. post-operatively she made steady progress for 5 days although still requiring nasal ventilatory support. thereaf ... | 1997 | 9203887 |
| subdural empyema due to burkholderia cepacia: an unusual complication after lung transplantation for cystic fibrosis. | | 1997 | 9204013 |
| structural elucidation of two polysaccharides present in the lipopolysaccharide of a clinical isolate of burkholderia cepacia. | based on the sugar composition, methylation analyses and smith degradation, supported by nmr spectroscopic analyses and fast-atom-bombardment ms experiments, the lipopolysaccharide produced by a clinical isolate of burkholderia cepacia was shown to contain two distinct polymers, both with linear trisaccharide repeating units; a major, containing d-rhamnose and d-galactose residues (2:1) with the structure -->3)-alpha-d-rhap(1-->3)-alpha-d-rhap(1-->4)-alpha-d-galp(1 --> (major), and a minor repea ... | 1997 | 9208925 |
| comparative activity of trovafloxacin, alone and in combination with other agents, against gram-negative nonfermentative rods. | in the first part of this study, agar dilution mics were used to test the activities of trovafloxacin, ciprofloxacin, ofloxacin, levofloxacin, sparfloxacin, clinafloxacin, ceftazidime, and imipenem against 458 gram-negative nonfermenters. the overall respective mics at which 50% of isolates are inhibited (mic50s) and mic90s were as follows: trovafloxacin, 1.0 and 16.0 microg/ml; ciprofloxacin, 2.0 and 16.0 microg/ml; ofloxacin, 2.0 and 32.0 microg/ml; levofloxacin, 1.0 and 16.0 microg/ml; sparfl ... | 1997 | 9210669 |
| a novel means to develop strain-specific dna probes for detecting bacteria in the environment. | a simple means to develop strain-specific dna probes for use in monitoring the movement and survival of bacteria in natural and laboratory ecosystems was developed. the method employed amplification of genomic dna via repetitive sequence-based pcr (rep-pcr) using primers specific for repetitive extragenic palindromic (rep) elements, followed by cloning of the amplified fragments. the cloned fragments were screened to identify those which were strain specific, and these were used as probes for to ... | 1997 | 9212434 |
| porin from the halophilic species ectothiorhodospira vacuolata: cloning, structure of the gene and comparison with other porins. | the gene coding for the anion-specific porin of the halophilic eubacterium ectothiorhodospira (ect.) vacuolata was cloned and sequenced, the first such gene so analyzed from a purple sulfur bacterium. it encodes a precursor protein consisting of 374 amino acid (aa)-residues including a signal peptide of 22-aa residues. comparison with aa sequences of porins from several other members of the proteobacteria revealed little homology. only two regions showed local homology with the previously sequen ... | 1997 | 9218724 |
| genotypic analysis of burkholderia cepacia isolates from 13 french cystic fibrosis centers. | burkholderia cepacia has been involved in outbreaks of pulmonary infection among patients with cystic fibrosis (cf), and the spread of a highly transmissible clone has been reported throughout the united kingdom and canada. these data prompted a dna-based typing study of the strains recovered in french cf centers. ninety-five isolates recovered from 71 patients attending 13 cf centers in 9 regions of france were characterized by randomly amplified polymorphic dna (rapd) analysis and pulsed-field ... | 1997 | 9230381 |
| use of bronchoalveolar lavage semiquantitative cultures in cystic fibrosis. | to assess bronchoalveolar lavage (bal) in adult cf patients with respiratory symptoms, we studied bal fluid (balf) culture results from 28 bronchoscopies in 11 patients. patients were asked to provide sputum for culture. all but two patients were receiving antibiotics at the time of bronchoscopy, with 13 bronchoscopies done on patients who had been receiving antibiotics for more than 10 d. gram stain of the balf was positive in 18 cases. in all but one balf, > 10,000 colony-forming units per mil ... | 1997 | 9230762 |
| ribotyping and dna macrorestriction analysis of isolates of burkholderia pseudomallei from cases of melioidosis in malaysia. | forty-nine isolates of burkholderia pseudomallei from sporadic cases of melioidosis in malaysia over the past 18 years were examined by bamhi ribotyping and pulsed-field gel electrophoresis (pfge) of xbai digests of total deoxyribonucleic acid (dna). twenty-four patients had septicaemic melioidosis with a mortality of 70%; mortality in the non-septicaemic disease was 16%. five ribotype patterns were identified, 2 of which accounted for 90% of all isolates. pfge revealed a number of different str ... | 1997 | 9231217 |
| toxin production by burkholderia pseudomallei strains and correlation with severity of melioidosis. | an exotoxin lethal to cells in culture (cytolethal toxin, clt) was identified in culture filtrates of burkholderia pseudomallei, the causative organism of melioidosis. clt could pass through a 10-kda cut-off ultrafilter and its properties suggest that it is a peptide. isolates from soil, animals and man showed differential cytolethality in vitro. the isolates were divided into low, medium and high clt producers with soil isolates being low producers and isolates from patients with melioidosis en ... | 1997 | 9236739 |
| ribotype differences between clinical and environmental isolates of burkholderia pseudomallei. | burkholderia pseudomallei is isolated frequently from the soil in regions where the disease melioidosis occurs. however, recent surveys in thailand have shown that the frequency of isolation of the organism from soil samples is not directly related to the incidence of melioidosis in an area. to determine whether strain populations of b. pseudomallei prevalent in soil are gentypically related to strains causing clinical disease, rrna bamhi restriction fragment length polymorphisms (rflp) of 139 s ... | 1997 | 9236740 |
| the clonal antibody response to pseudomonas aeruginosa heat shock protein is highly diverse in cystic fibrosis patients. | the groel protein of pseudomonas aeruginosa belongs to the bacterial 60-65 kda heat shock protein family. a strong antibody response to groel has been found in cystic fibrosis (cf) patients with chronic pulmonary infection caused by p. aeruginosa. clonotypes of igg1 and igg2 antibodies against groel were studied in 60 consecutive sera from 18 cf patients with chronic p. aeruginosa infection using isoelectric focusing in combination with affinity immunoblotting. the persistent antigenic stimulati ... | 1997 | 9236862 |
| activity of pseudomonas cepacia lipase in organic media is greatly enhanced after immobilization on a polypropylene support. | the purified lipase from pseudomonas cepacia was used as free and immobilized enzyme preparation for hydrolysis of p-nitrophenyl palmitate (pnpp) and p-nitrophenyl acetate (pnpa) in organic media. the free enzyme was mixed with bovine serum albumin and lyophilized. immobilization was on porous polypropylene. conditions where diffusional limitations of the substrate were not limiting the reaction rate were defined. the specific activity of the lipase was greatly enhanced upon immobilization: 16.5 ... | 1997 | 9237387 |
| speed of detection of burkholderia pseudomallei in blood cultures and its correlation with the clinical outcome. | burkholderia pseudomallei is a major cause of fatalities from nonhospital-acquired, gram-negative bacterial septicemia in northeastern part of thailand. rapid isolation of the bacterium is critical for diagnosis and treatment. bacterial culture is currently the gold standard method for laboratory diagnosis of melioidosis. the present study describes the time to detection of b. pseudomallei in blood cultures using a bact/alert automated blood culture system, and the correlation between the speed ... | 1997 | 9242327 |
| first case report of melioidosis in guadeloupe, a french west indies archipelago. | | 1997 | 9243060 |
| [the pathomorphology and pathogenesis of glanders in laboratory animals]. | the pathomorphology and cell-mediated response of the body to burkholderia mallei in laboratory animals, highly sensitive and resistant to these bacteria. in the comparative study of the pathomorphology and pathogenesis of glanders in golden hamsters and white rats quantitative and qualitative differences in the histogenesis of response reaction and the morphology of immunocompetent organs were established. cell-mediated reactions play a greater role in the limitation of the early spread of b. m ... | 1997 | 9245146 |
| genetic and biochemical characterization of the broad spectrum chlorobenzene dioxygenase from burkholderia sp. strain ps12--dechlorination of 1,2,4,5-tetrachlorobenzene. | the bacterium, burkholderia (previously pseudomonas) sp. strain ps12, reported earlier to degrade 1,2,4-trichlorobenzene is shown here to utilize also 1,2,4,5-tetrachlorobenzene (cl4-benzene) as a growth substrate. to investigate the possibility that this organism attacks cl4-benzene with a chlorobenzene dioxygenase which concomitantly causes dehalogenation, and to analyze the substrate range of the initial enzyme, a 5503-bp dna fragment from ps12, exhibiting high similarity to genes coding for ... | 1997 | 9249026 |
| low bacterial contamination of nebulizers in home treatment of cystic fibrosis patients. | many reports have shown bacterial contamination of nebulizers used by patients with cystic fibrosis (cf) at home. at the stockholm cf centre we recommend dismantling the equipment, washing, rinsing and drying it after each use, and disinfecting it once daily by boiling water or by 2% acetic acid followed by drying without rinsing. we studied whether patients comply with these recommendations and whether they are sufficient to prevent bacterial contamination. nebulizers from 49 cf patients were i ... | 1997 | 9253701 |
| microbiologic data overview of italian cystic fibrosis patients. | the aim of our study was to determine the frequency of microbiological culturing and prevalence of colonization by principal pathogens of the respiratory tract of italian cystic fibrosis patients. data on all italian cystic fibrosis patients were collected using a questionnaire sent to all italian cf centers. results were obtained of microbiological cultures from 2,521 patients. information was gained regarding the method of gathering biological samples, the percentage of patients undergoing mic ... | 1997 | 9258532 |
| [pulmonary melioidosis in a german southeast asia tourist]. | a patient who returned from a 3-year stay in thailand and india one year ago, was admitted with fever of 38.5 degrees c and productive cough for the last four weeks. he remembered wounding his foot three years ago in india with contamination by soil. subsequently, recurrent pustulae appeared on his feet. one such pustule was found on admittance. the clinical examination showed low body weight, without further abnormalities. | 1997 | 9265152 |
| comparative in vitro susceptibility of burkholderia pseudomallei to cefpirome, ceftazidime and cefepime. | | 1997 | 9271030 |
| abnormal technetium labelled white cell scan in the colitis of chronic granulomatous disease. | a child with colitis was treated for crohn's disease, diagnosed on history, clinical and colonoscopic findings, radiolabelled white cell bowel scan, and colonic histology. after septicaemia caused by an unusual organism, further investigation lead to a diagnosis of chronic granulomatous disease (cgd). the granulomatous colitis of cgd is clinically, histologically, and on white cell scanning, indistinguishable from that in crohn's disease and should be considered in atypical cases. infection with ... | 1997 | 9279152 |
| indirect hemagglutination antibodies against burkholderia pseudomallei in normal blood donors and suspected cases of melioidosis in malaysia. | interpretation of the indirect hemagglutination test (iha) for melioidosis in endemic areas is difficult because of the presence of antibodies in apparently healthy individuals. fifty-three out of 200 healthy blood donors in malaysia showed positive antibody titers (> or = 1 : 40) against burkholderia pseudomallei. seven percent had an iha titer of 1 : 40, 11% had an iha titer of 1 : 80 while 8.5% had a titer > or = 1 : 160. out of 258 sera sent for melioidosis serology, 7% of the patients had a ... | 1996 | 9279987 |
| meliodosis in india. | | 1996 | 9282627 |
| specificity and functional activity of anti-burkholderia pseudomallei polysaccharide antibodies. | the lipopolysaccharide (lps) of burkholderia pseudomallei, the causative agent of melioidosis, consists of two o-antigenic polysaccharides designated o-ps i and o-ps ii. in this study, the o-ps specificity and functional activity of a protective polyclonal antiserum and an immunoglobulin m (igm) monoclonal antibody were determined. the polyclonal antiserum recognized both o-ps i and o-ps ii, while the monoclonal antibody was o-ps ii specific. both mediated phagocytic killing of b. pseudomallei b ... | 1997 | 9284132 |
| [immunogenic potential of glanders and melioidosis agents]. | unlike the glanders agent, the superficial structures of the melioidosis agent were demonstrated to be responsible for marked was immunosuppressive activity. some antigenic fractions suppressing the blast transformation of lymphocytes, reducing the count of t helpers and profoundly potentiating the infection in vivo were isolated from p. pseudomallei cells. the immunogenic and immunosuppressive activities of both agents' superficial structures were studied by high performance chromatography. ant ... | 1997 | 9289277 |
| [effect of tra-system of plasmids rp4 and r68.45 on pseudomonas mallei virulence]. | in pseudomonas mallei, spontaneous mutants (tra- mutants) of the plasmids rp4 and r68.45, losing the ability to transfer at conjugation are formed. the plasmids rp4 and r68.45 with tra(+)-phenotype caused a decrease in p. mallei virulence for laboratory animals. at the same time, tra- mutants of these plasmids do not affect p. mallei virulence. the insertion of dna fragment of about 1900 bp into the plasmid transfer gene regions (tra-2-tra-3) gave rise to rp4 and r68.45 tra mutations detectable ... | 1997 | 9289278 |
| cross-regulation of toluene monooxygenases by the transcriptional activators tbmr and tbut. | the toluene-3-monooxygenase from burkholderia pickettii pko1 and the toluene/benzene-2-monooxygenase from burkholderia (pseudomonas) sp. strain js150 are distinct enzymes which differ not only in catalytic specificity and substrate range but also in the arrangement and sequence of the genes within the operons that encode the enzymes, tbua1ubva2c and tbmabcdef, respectively. in the present study, we examined the transcriptional activation of the ptbua1 and ptbma promoters by their cognate regulat ... | 1997 | 9293027 |
| characterization of the capsular polysaccharide of burkholderia (pseudomonas) pseudomallei 304b. | burkholderia (pseudomonas) pseudomallei is the causative agent of melioidosis, a bacterial infection of considerable morbidity in areas of endemicity of southeast asia and northern australia. clinical isolates of b. pseudomallei have been demonstrated to produce a lipopolysaccharide (lps) containing two separate and chemically distinct antigenic o polysaccharides against which infected patients produced antibodies. a putative capsular polysaccharide (cps) has also been reported and is thought to ... | 1997 | 9294419 |
| [melioidosis: a tropical time bomb that is spreading]. | melioidosis, an infectious disease that affects many mammals, was first identified in burma by whitmore in 1912. it is caused by burkholderia pseudomallei, a gram negative bacillus of the pseudomonas family, which is found in soil and water. long present in southeast asia and numerous tropical areas, melioidosis has recently appeared in temperate zones including mainland france. the incidence in endemic areas is between 6% and 20% of the population and short period of exposure is sufficient to b ... | 1997 | 9304017 |
| [the transfer of antibacterial resistance to recipients highly sensitive to glanders]. | in multiple experiments on golden hamsters, highly sensitive to glanders, the method of the transfer of antibacterial resistance was worked out. the most positive effect was obtained after the subcutaneous injection of saline-suspended marrow cells, obtained from white rats, infected with burkholderia mallei c5 in a single inoculation and passaged through noninfected white rats 5 times. out of 60 golden hamsters, treated with such material and subsequently (on day 21) injected with 1.0 x 10(2) b ... | 1997 | 9304339 |
| mobilization, cloning, and sequence determination of a plasmid-encoded polygalacturonase from a phytopathogenic burkholderia (pseudomonas) cepacia. | phytopathogenic strains of burkholderia cepacia (synonym pseudomonas cepacia) produce endopolygalacturonase, whereas strains of clinical and soil origin do not. growth of a phytopathogenic strain (atcc25416) at elevated temperatures resulted in nonpectolytic derivatives that were either cured of a resident plasmid or contained a plasmid of reduced mass. the resident 200-kb plasmid (ppec320) in strain atcc25416 was tagged with tn5-mob. the ppec320::tn5-mob (ppec321) plasmid was mobilized in b. ce ... | 1997 | 9304858 |
| identification by replicon-typing of indigenous plasmids of burkholderia (pseudomonas) solanacearum. | fifteen strains of burkholderia (pseudomonas) solanacearum were isolated, identified from different parts of india and screened for plasmid profile. they harboured 1-3 plasmids, but most of the isolates contained a single plasmid. plasmids were classified on the basis of replicon typing by southern hybridization using incompatible specific rep probes. plasmids of 9 strains hybridized with repp probe, plasmids of 5 strains hybridized with repw probe and one plasmid hybridized with repq probe. fin ... | 1997 | 9315243 |
| role of reactive nitrogen and oxygen intermediates in gamma interferon-stimulated murine macrophage bactericidal activity against burkholderia pseudomallei. | we examined the contributions of reactive nitrogen and oxygen intermediates (rni and roi, respectively) in macrophage bactericidal activity against burkholderia pseudomallei, the causative agent of melioidosis, in order to understand host defense mechanisms against infection caused by this bacterium. the bacteria multiplied in unstimulated murine macrophage cell line j774.1. however, a strong dose-dependent inhibition of intracellular bacterial growth was observed when gamma interferon (ifn-gamm ... | 1997 | 9317015 |
| priming of neutrophil respiratory burst activity by lipopolysaccharide from burkholderia cepacia. | neutrophil activation may play an important role in the pathogenesis of respiratory disease in burkholderia cepacia-colonized cystic fibrosis (cf) patients. as bacterial lipopolysaccharides (lps) are potent immunostimulatory molecules, we investigated the role of b. cepacia lps in neutrophil activation processes. lps extracted from a highly transmissible and virulent strain of b. cepacia (j2315) was found to increase neutrophil surface expression of the beta2 integrin, complement receptor 3, and ... | 1997 | 9317038 |
| arabinose assimilation defines a nonvirulent biotype of burkholderia pseudomallei. | two distinct types of burkholderia pseudomallei, differentiated by the ability to assimilate l-arabinose but with similar morphologies and antigenicities, can be isolated from soil in thailand. approximately 25% of soil isolates from northeast thailand were arabinose assimilators (ara+), but in 1,200 sequentially studied patients, only arabinose "nonassimilators" (ara-) caused melioidosis (p < 0.0001). in a murine model, there was a striking difference in virulence between ara- and ara+ b. pseud ... | 1997 | 9317042 |
| isolation rates of burkholderia pseudomallei among the four regions in thailand. | this study aimed to compare the isolation rates of burkholderia pseudomallei among community-based hospitals located in the central, north, northeast, and south of thailand. a questionnaire inquiring about the number of isolation of b. pseudomallei from various clinical specimens during 1994-95 were mailed to 141 community-based hospitals. of these, 125 hospitals (88.6%) responded to the questionnaire. microbiological laboratory was not available in thirty hospitals. data from 95 remaining hospi ... | 1997 | 9322292 |
| isolation of new 6-methylnicotinic-acid-degrading bacteria, one of which catalyses the regioselective hydroxylation of nicotinic acid at position c2. | 2-hydroxynicotinic acid is an important building block for herbicides and pharmaceuticals. enrichment strategies to increase the chances of finding microorganisms capable of hydroxylating at the c2 position and to avoid the degradation of nicotinic acid via the usual intermediate, 6-hydroxynicotinic acid, were used. three bacterial strains (mena 23/3-3c, mena 25/4-1, and mena 25/ 4-3) were isolated from enrichment cultures with 6-methylnicotinic acid as the sole source of carbon and energy. part ... | 1997 | 9325423 |
| a review of the in vitro activity of meropenem and comparative antimicrobial agents tested against 30,254 aerobic and anaerobic pathogens isolated world wide. | the in vitro activity of meropenem (formerly sm-7738), a new carbapenem, was compared with that of imipenem and five other broad-spectrum antimicrobials (ceftazidime, cefotaxime, piperacillin, piperacillin/tazobactam, and ciprofloxacin) against 30,254 clinically significant pathogens isolated in nine countries worldwide. overall, the carbapenems, meropenem and imipenem, were the most active drugs. meropenem was four- to 64-fold more active than imipenem against gram-negative bacteria, including ... | 1997 | 9327242 |
| in vitro activities of antimicrobial agents, alone and in combinations, against burkholderia cepacia isolated from blood. | burkholderia cepacia is a widespread, environmental gram-negative bacillus that is associated with nosocomial infections. this bacterium is considered to be an important pathogen in immunocompromised patients and is inherently resistant to multiple antimicrobial agents. to compare the activity of different antimicrobial agents and the potential of combinations against invasive strains of b. cepacia, we collected 36 isolates of b. cepacia from blood cultures and checked their susceptibilities to ... | 1997 | 9327247 |
| multiple pathways for toluene degradation in burkholderia sp. strain js150. | burkholderia (pseudomonas) sp. strain js150 uses multiple pathways for the metabolism of catechols that result from degradation of aromatic compounds. this suggests that the strain also uses multiple upstream pathways for the initial hydroxylation of aromatic substrates. two distinct dna fragments that allowed pseudomonas aeruginosa pao1c to grow with benzene as a sole carbon source were cloned from strain js150. one of the recombinant plasmids containing the initial steps for the degradative pa ... | 1997 | 9327568 |
| occurrence of multiple genomovars of burkholderia cepacia in cystic fibrosis patients and proposal of burkholderia multivorans sp. nov. | we performed an integrated genotypic and phenotypic analysis of 128 strains of the genera burkholderia, ralstonia, and pseudomonas in order to study the taxonomic structure of burkholderia cepacia and its relationships with other burkholderia species. our data show that presumed b. cepacia strains isolated from cystic fibrosis patients belong to at least five distinct genomic species, one of which was identified as burkholderia vietnamiensis. this group of five phenotypically similar species is ... | 1997 | 9336927 |
| should patients with cystic fibrosis infected with burkholderia cepacia undergo lung transplantation? | | 1997 | 9337823 |
| genetic relatedness of burkholderia (pseudomonas) cepacia isolates from five cystic fibrosis centers in michigan. | burkholderia cepacia isolates from patients with cystic fibrosis (cf) attending five cf centers were studied for relatedness by cellular fatty acid methyl esters (fame) and by chromosomal dna restriction analysis. twenty-eight of 32 (87.5%) isolates tested were grouped in cluster group 1 based on their fame profiles. dna analysis revealed that 29 of 32 (90.6%) b. cepacia isolates from five cf centers had one closely related dna pattern. to examine strain variation over a time period, fame profil ... | 1997 | 9338052 |
| enhancement of burkholderia cepacia antimicrobial susceptibility by cationic compounds. | infections in cystic fibrosis (cf) due to burkholderia cepacia are challenging due to their resistance to antibiotics. we explored a new strategy for increasing the permeability of b. cepacia using cationic agents, including amino compounds, to reduce the mics of standard antibiotics. twenty-eight b. cepacia isolates from four cf centres in north america and four non-cf b. cepacia were examined by standard microtitre broth dilution methods for susceptibility to a variety of antibiotics in the pr ... | 1997 | 9338485 |
| influence of growth rate and nutrient limitation on susceptibility of burkholderia cepacia to ciprofloxacin and tobramycin. | the influence of growth rate and oxygen availability on ciprofloxacin and tobramycin sensitivity and cell surface hydrophobicity in burkholderia cepacia was assessed for cells grown in a chemostat under iron-limitation. whereas susceptibility to both antibiotics decreased with increasing growth rate and oxygen repletion yet increased under oxygen depletion, hydrophobicity decreased with increasing growth rate and oxygen repletion but was relatively unaffected under oxygen depletion. these result ... | 1997 | 9338495 |
| the influence of moisture on microbial transport, survival and 2,4-d biodegradation with a genetically marked burkholderia cepacia in unsaturated soil columns. | the influence of moisture on the survival, movement and degradation activity of a 2,4-d degrading bacterium, burkholderia cepacia strain bri6001l, genetically engineered to contain bioluminescent and lactose utilization genes, was studied in unsaturated soil columns. the distance traveled by bri6001l was dependent on the clay content of the soil, higher clay contents being responsible for higher filtration coefficients. long term survival, in excess of one year, was attributed to strain bri6001l ... | 1997 | 9342881 |
| evaluation of preservative effectiveness in pharmaceutical products: the use of a wild strain of pseudomonas cepacia. | a sodium benzoate-sorbic acid preservative system of a pharmaceutical product was proved effective against a wild strain of pseudomonas cepacia, following the official method of the italian and british pharmacopoeias. however, this preservative system was ineffective against a challenge of ps. cepacia wild strain cells grown in the unpreserved pharmaceutical product and on culture media different from those described by the pharmacopoeias. the adaptive resistance of the wild strain of ps. cepaci ... | 1997 | 9351212 |
| structural studies of the o-specific side-chain of lipopolysaccharide from burkholderia gladioli pv. gladioli strain ncppb 1891. | a polymeric fraction (the o-antigenic side-chain) has been isolated from the lipopolysaccharide of burkholderia gladioli pv. gladioli strain ncppb 1891 after mild acid hydrolysis. the components of the polymer and their molar proportions were l-rha (1), d-gal (1), d-man (1), and o-acetyl (1). by means of chemical degradations and nmr studies, the repeating unit of the polymer was shown to be a linear trisaccharide of the structure shown. [formula: see text] | 1997 | 9352638 |
| four additional cases of burkholderia gladioli infection with microbiological correlates and review. | burkholderia gladioli has only recently been reported to be a human pathogen. four cases of b. gladioli infection (including bacteremia, pneumonia, and cervical adenitis) in two adults and two young children are reported. three of these four patients were severely immunocompromised. commercial systems were frequently unable to identify this bacterium correctly. antimicrobial susceptibility patterns indicated that b. gladioli strains were susceptible to the quinolones, aminoglycosides, and imipen ... | 1997 | 9356798 |