| diversity of 16s-23s rdna internal transcribed spacer (its) reveals phylogenetic relationships in burkholderia pseudomallei and its near-neighbors. | length polymorphisms within the 16s-23s ribosomal dna internal transcribed spacer (its) have been described as stable genetic markers for studying bacterial phylogenetics. in this study, we used these genetic markers to investigate phylogenetic relationships in burkholderia pseudomallei and its near-relative species. b. pseudomallei is known as one of the most genetically recombined bacterial species. in silico analysis of multiple b. pseudomallei genomes revealed approximately four homologous r ... | 2011 | 22195045 |
| discovery of dna operators for tetr and marr family transcription factors fromburkholderia xenovorans. | determining transcription factor recognition motifs or operator sites is central to understanding gene regulation, yet few operators have been characterized. in this study, we used a protein-binding microarray (pbm) to discover the dna recognition sites and putative regulons for three tetr and one marr family tfs derived from b. xenovorans, which are common to the burkholderia genus. we also describe the development and application of a more streamlined version of the pbm technology that signifi ... | 2011 | 22117006 |
| O-Acetyl location on cepacian, the principal exopolysaccharide of Burkholderia cepacia complex bacteria. | Cepacian is an exopolysaccharide produced by the majority of the isolates belonging to the Burkholderia cepacia complex bacteria, a group of 17 species, some of which infect cystic fibrosis patients, sometime with fatal outcome. The repeating unit of cepacian consists of a backbone having a trisaccharidic repeating unit with three side chains, as reported in the formula below. The exopolysaccharide is also acetylated, carrying from one to three acetyl esters per repeating unit, depending on the ... | 2011 | 22055818 |
| The CRP/FNR family protein Bcam1349 is a c-di-GMP effector that regulates biofilm formation in the respiratory pathogen Burkholderia cenocepacia. | Burkholderia cenocepacia is an opportunistic respiratory pathogen that can cause severe infections in immune-compromised individuals and is associated with poor prognosis for patients suffering from cystic fibrosis. The second messenger cyclic diguanosine monophosphate (c-di-GMP) has been shown to control a wide range of functions in bacteria, but little is known about these regulatory mechanisms in B. cenocepacia. Here we investigated the role that c-di-GMP plays in the regulation of biofilm fo ... | 2011 | 21883527 |
| Antioxidant compounds improved PCB-degradation by Burkholderia xenovorans strain LB400. | Polychlorobiphenyls (PCBs) are toxic and persistent organic pollutants that are widely distributed in the environment. Burkholderia xenovorans LB400 is capable of degrading aerobically an unusually wide range of PCBs. However, during PCB-degradation B. xenovorans LB400 generates reactive oxygen species (ROS) that affect its viability. The aim of this study was to increase the efficiency of PCB-degradation of B. xenovorans LB400 by adding antioxidant compounds that could increase tolerance to oxi ... | 2011 | 22142725 |
| Bacterial leaf symbiosis in angiosperms: host specificity without co-speciation. | Bacterial leaf symbiosis is a unique and intimate interaction between bacteria and flowering plants, in which endosymbionts are organized in specialized leaf structures. Previously, bacterial leaf symbiosis has been described as a cyclic and obligate interaction in which the endosymbionts are vertically transmitted between plant generations and lack autonomous growth. Theoretically this allows for co-speciation between leaf nodulated plants and their endosymbionts. We sequenced the nodulated Bur ... | 2011 | 21915326 |
| association of dnt genes of burkholderia sp. dnt with the substrate-blind regulator dntr draws the evolutionary itinerary of 2,4-dinitrotoluene biodegradation. | the regulation of the dnt pathway for biodegradation of 2,4-dinitrotoluene of burkholderia sp. dnt has been examined by exporting each of its components to pseudomonas putida kt2440. the cognate regulator dntr does not respond to the pathway substrate, but to the non-substrate salicylate. in order to examine whether such a response to an unrelated inducer was specific or rather a vestige of a previous evolutionary stage, the complete dnt complement or parts of it were expressed functionally for ... | 2011 | 21923773 |
| enantiomeric scaffolding of α-tetralone and related scaffolds by ekr (enzymatic kinetic resolution) and stereoselective ketoreduction with ketoreductases. | stereochemically pure compounds containing an all carbon quaternary stereocenter based on 1-tetralone, 1-indanone and 4-chromanone scaffolds have been synthesized by employing lipase ps (burkholderia cepacia) catalyzed kinetic resolution. these scaffolds are further functionalized by microbial ketoreductase enzymes (geotrichum candidum, candida parapsilosis and aspergillus niger) to access stereochemically pure diols which, on further synthetic manipulation, yield novel cyclic compounds. | 2011 | 22102058 |
| Burkholderia phytofirmans strain PsJN primes Vitis vinifera L. and confers a better tolerance to low non-freezing temperatures. | Several endophytic bacteria reportedly induce resistance to biotic stress and abiotic stress tolerance in several plant species. Burkholderia phytofirmans strain PsJN (PsJN) is a plant growth-promoting rhizobacterium (PGPR) that is able to colonize grapevine tissues and induce resistance to gray mold. Further, PsJN induces physiological changes that increase grapevine tolerance to low non-freezing temperatures. To better understand how bacteria induced the observed phenomena, stress-related gene ... | 2011 | 21942451 |
| transcriptional responses of burkholderia cenocepacia to polymyxin b in isogenic strains with diverse polymyxin b resistance phenotypes. | burkholderia cenocepacia is a gram-negative opportunistic pathogen displaying high resistance to antimicrobial peptides and polymyxins. we identified mechanisms of resistance by analyzing transcriptional changes to polymyxin b treatment in three isogenic b. cenocepacia strains with diverse polymyxin b resistance phenotypes: the polymyxin b-resistant parental strain k56-2, a polymyxin b-sensitive k56-2 mutant strain with heptoseless lipopolysaccharide (lps) (rsf34), and a derivative of rsf34 (rsf ... | 2011 | 21955326 |
| effectiveness of a simplified method for isolation of burkholderia pseudomallei from soil. | detection of environmental burkholderia pseudomallei indicates a risk for melioidosis and is important for the development of a global risk map. we describe a simple method for detecting b. pseudomallei using direct culture of soil in enrichment broth. this gives a rate of positivity comparable to that obtained with a standard method but is cheaper and labor saving. | 2011 | 22101048 |
| Probing conformational states of glutaryl-CoA dehydrogenase by fragment screening. | Glutaric acidemia type 1 is an inherited metabolic disorder which can cause macrocephaly, muscular rigidity, spastic paralysis and other progressive movement disorders in humans. The defects in glutaryl-CoA dehydrogenase (GCDH) associated with this disease are thought to increase holoenzyme instability and reduce cofactor binding. Here, the first structural analysis of a GCDH enzyme in the absence of the cofactor flavin adenine dinucleotide (FAD) is reported. The apo structure of GCDH from Burkh ... | 2011 | 21904051 |
| common features of environmental and potentially beneficial plant-associated burkholderia. | the genus burkholderia comprises more than 60 species isolated from a wide range of niches. although they have been shown to be diverse and ubiquitously distributed, most studies have thus far focused on the pathogenic species due to their clinical importance. however, the increasing number of recently described burkholderia species associated with plants or with the environment has highlighted the division of the genus into two main clusters, as suggested by phylogenetical analyses. the first c ... | 2011 | 21850446 |
| Structures of Burkholderia thailandensis nucleoside kinase: implications for the catalytic mechanism and nucleoside selectivity. | The nucleoside kinase (NK) from the mesophilic Gram-negative bacterium Burkholderia thailandensis (BthNK) is a member of the phosphofructokinase B (Pfk-B) family and catalyzes the Mg(2+)- and ATP-dependent phosphorylation of a broad range of nucleosides such as inosine (INO), adenosine (ADO) and mizoribine (MZR). BthNK is currently used in clinical practice to measure serum MZR levels. Here, crystal structures of BthNK in a ligand-free form and in complexes with INO, INO-ADP, MZR-ADP and AMP-Mg( ... | 2011 | 22101821 |
| [pneumonia-causing bacterial pathogens in intensive care patients]. | this prospective study aimed at determining etiologic agents causing nosocomial pneumonia in a precisely defined group of patients and resistance of bacterial pathogens to antimicrobial drugs. | 2011 | 22052100 |
| Enhancing plant disease suppression by Burkholderia vietnamiensis through chromosomal integration of Bacillus subtilis chitinase gene chi113. | Burkholderia vietnamiensis P418 is a plant growth-promoting rhizobacteria. A chitinase gene from Bacillus subtilis was cloned and stably integrated into the chromosome of using the transposon delivery vector, pUTkm1. Chitinase activity was detected in recombinant P418-37 but not in wild type P418. Recombinant P418-37 retained the in vitro growth rate, N(2)-fixation and phosphate and potassium-solubilizing characteristics of the wild type. P418-37 significantly (P < 0.05) inc ... | 2011 | 21972146 |
| development of an acute model of inhalational melioidosis in the common marmoset (callithrix jacchus). | studies of inhalational melioidosis were undertaken in the common marmoset (callithrix jacchus). following exposure to an inhaled challenge with aerosolized burkholderia pseudomallei, lethal infection was observed in marmosets challenged with doses below 10 cfu; a precise ld(50) determination was not possible. the model was further characterized using a target challenge dose of approximately 10(2) cfu. a separate pathogenesis time-course experiment was also conducted. all animals succumbed, betw ... | 2011 | 22122591 |
| combined docking and molecular dynamics simulations to enlighten the capacity of pseudomonas cepacia and candida antarctica lipases to catalyze quercetin acetylation. | a combined docking and molecular dynamics protocol was applied to investigate quercetin binding modes within the catalytic cavity of candida antarctica lipase b (calb) and pseudomonas cepacia lipase (pcl), aiming to explain the difference of specificity of these enzymes in acetylation reaction. for both lipases, docking of quercetin yielded two families of conformers with either the quercetin a or b-ring pointing towards the catalytic residues. molecular dynamics (md) calculations were subsequen ... | 2011 | 21933689 |
| should cystic fibrosis patients infected with burkholderia cepacia complex be listed for lung transplantation? | a best evidence topic was constructed according to a structured protocol. the question addressed was whether lung transplantation remained a beneficial treatment for cystic fibrosis (cf) patients infected or colonized with burkholderia cepacia complex (bcc) prior to lung transplantation (ltx). of the 25 papers found using a report search, five presented the best evidence to answer the clinical question. the authors, journal, date and country of publication, study type, group studied, relevant ou ... | 2011 | 21920934 |
| Regioselectivity-reversal in acylation of 6-azauridine catalyzed by Burkholderia cepacia lipase. | 3'-O-Stearoylation of 6-azauridine was achieved enzymatically for the first time. Among eight commercially available lipases, that from Burkholderia cepacia displayed a 3'-regioselectivity of 80% towards the acylation of 3-hydroxyl of 6-azauridine. Using an immobilized lipase from Burkholderia cepacia, the 3'-regioselectivities of the acylations could be reversed by lengthening the aliphatic chain of the acyl donors (C2-C18). The possible reason might be the presence of the interaction between t ... | 2012 | 21898129 |
| microvirga lupini sp. nov., microvirga lotononidis sp. nov., and microvirga zambiensis sp. nov. are alphaproteobacterial root nodule bacteria that specifically nodulate and fix nitrogen with geographically and taxonomically separate legume hosts. | strains of gram-negative, rod-shaped, non-spore-forming bacteria were isolated from nitrogen-fixing nodules of the native legumes listia angolensis (from zambia) and lupinus texensis (from texas, usa). phylogenetic analysis of the 16s rrna gene showed that the novel strains belong to the genus microvirga, with 96.1 % or greater sequence similarity with type strains of this genus. the closest relative of the representative strains lut6t and wsm3557t was m. flocculans tfbt, with 97.6-98.0 % simila ... | 2011 | 22199210 |
| characterization of the poly-β-1,6-n-acetylglucosamine polysaccharide component of burkholderia biofilms. | we demonstrated the production of poly-β-1,6-n-acetylglucosamine (pnag) polysaccharide in the biofilms of burkholderia multivorans, burkholderia vietnamiensis, burkholderia ambifaria, burkholderia cepacia, and burkholderia cenocepacia using an immunoblot assay for pnag. these results were confirmed by further studies, which showed that the pnag hydrolase, dispersin b, eliminated immunoreactivity of extracts from the species that were tested (b. cenocepacia and b. multivorans). dispersin b also i ... | 2011 | 21984237 |
| Expression of Aeromonas caviae polyhydroxyalkanoate synthase gene in Burkholderia sp. USM (JCM15050) enables the biosynthesis of SCL-MCL PHA from palm oil products. | Aims: Burkholderia sp. USM (JCM15050) isolated from oil-polluted wastewater is capable of utilizing palm oil products and glycerol to synthesize poly(3-hydroxybutyrate) [P(3HB)]. To confer the ability to produce polymer containing 3-hydroxyhexanoate (3HHx), plasmid (pBBREE32d13) harbouring the polyhydroxyalkanoate (PHA) synthase gene of Aeromonas caviae (phaC(Ac) ) was transformed into this strain. Methods and Results: The resulting transformant incorporated approximately 1 ± 0·3 mol% of 3HHx ... | 2012 | 22054430 |
| structure of 3-ketoacyl-(acyl-carrier-protein) reductase from rickettsia prowazekii at 2.25 å resolution. | rickettsia prowazekii, a parasitic gram-negative bacterium, is in the second-highest biodefense category of pathogens of the national institute of allergy and infectious diseases, but only a handful of structures have been deposited in the pdb for this bacterium; to date, all of these have been solved by the ssgcid. owing to its small genome (about 800 protein-coding genes), it relies on the host for many basic biosynthetic processes, hindering the identification of potential antipathogenic drug ... | 2011 | 21904060 |
| myd88-dependent recruitment of monocytes and dendritic cells required for protection from pulmonary burkholderia mallei infection. | the gram-negative bacterium burkholderia mallei causes rapidly fatal illness in equines and humans when contracted by inhalation and also has the potential to be used as a bioweapon. however, little is known regarding the early innate immune responses and signaling mechanisms required to generate protection from pneumonic b. mallei infection. we showed previously that monocyte chemoattractant protein 1 (mcp-1) was a critical chemokine required for protection from pneumonic b. mallei infection. w ... | 2012 | 22025508 |
| impact of conditioning films on the initial adhesion of burkholderia cepacia. | bacterial initial adhesion to inert surfaces in aquatic environments is mainly governed by the surface properties of the substratum, which can be altered significantly by the formation of conditioning films. bacteria were tested for ability to adhere to bare glass slides and to slides coated with alginate, bovine serum albumin (bsa), or suwannee river natural organic matter (sr-nom). three burkholderia cepacia strains with different extracellular polymeric substance (eps) secretion capacities we ... | 2012 | 22112498 |
| chance and necessity in the evolution of a bacterial pathogen. | the combination of genomic, epidemiological and evolutionary analyses provides a powerful toolbox for understanding how pathogens adapt to their human hosts. by sequencing 112 burkholderia dolosa genomes from an outbreak among patients with cystic fibrosis, a new study documents evolution in action and identifies a set of genes that contributed to the pathogen's adaptation. | 2011 | 22120052 |
| functional characterization and biological significance of yersinia pestis lipopolysaccharide biosynthesis genes. | in silico analysis of available bacterial genomes revealed the phylogenetic proximity levels of enzymes responsible for biosynthesis of lipopolysaccharide (lps) of yersinia pestis, the cause of plague, to homologous proteins of closely related yersinia spp. and some other bacteria (serratia proteamaculans, erwinia carotovora, burkholderia dolosa, photorhabdus luminescens and others). isogenic y. pestis mutants with single or double mutations in 14 genes of lps biosynthetic pathways were construc ... | 2011 | 21999543 |
| The NLRC4 inflammasome receptors for bacterial flagellin and type III secretion apparatus. | Inflammasomes are large cytoplasmic complexes that sense microbial infections/danger molecules and induce caspase-1 activation-dependent cytokine production and macrophage inflammatory death. The inflammasome assembled by the NOD-like receptor (NLR) protein NLRC4 responds to bacterial flagellin and a conserved type III secretion system (TTSS) rod component. How the NLRC4 inflammasome detects the two bacterial products and the molecular mechanism of NLRC4 inflammasome activation are not understoo ... | 2011 | 21918512 |
| Hydroxysteroid dehydrogenases (HSDs) in bacteria - A bioinformatic perspective. | Steroidal compounds including cholesterol, bile acids and steroid hormones play a central role in various physiological processes such as cell signaling, growth, reproduction, and energy homeostasis. Hydroxysteroid dehydrogenases (HSDs), which belong to the superfamily of short-chain dehydrogenases/reductases (SDR) or aldo-keto reductases (AKR), are important enzymes involved in the steroid hormone metabolism. HSDs function as an enzymatic switch that controls the access of receptor-active stero ... | 2011 | 21884790 |
| in vivo bioluminescence imaging of burkholderia mallei respiratory infection and treatment in the mouse model. | bioluminescent imaging (bli) technology is a powerful tool for monitoring infectious disease progression and treatment approaches. bli is particularly useful for tracking fastidious intracellular pathogens that might be difficult to recover from certain organs. burkholderia mallei, the causative agent of glanders, is a facultative intracellular pathogen and has been classified by the cdc as a category b select agent due to its highly infectious nature and potential use as a biological weapon. ve ... | 2011 | 21904535 |
| Proteomic analysis of colony morphology variants of Burkholderia pseudomallei defines a role for the arginine deiminase system in bacterial survival. | Colony morphology variation of Burkholderia pseudomallei is a notable feature of a proportion of primary clinical cultures from patients with melioidosis. Here, we examined the hypothesis that colony morphology switching results in phenotypic changes associated with enhanced survival under adverse conditions. We generated isogenic colony morphology types II and III from B. pseudomallei strain 153 type I, and compared their protein expression profiles using 2D gel electrophoresis. Numerous protei ... | 2012 | 22062159 |
| Contemporary unconventional clinical use of co-trimoxazole. | Clin Microbiol Infect 2012; 18: 8-17 ABSTRACT: In the late 1960s, the combination of trimethoprim and sulphamethoxazole (co-trimoxazole) was introduced into clinical practice and used to treat many infectious diseases, such as urinary tract infections, respiratory infections, sexually transmitted diseases, Gram-negative sepsis, enteric infections and typhoid fever. Subsequently, co-trimoxazole was reported to be effective against numerous bacterial, fungal and protozoal pathogens, including Noca ... | 2012 | 21851485 |
| burkholderia symbiotica sp. nov., isolated from root nodules of mimosa spp. native to north east brazil. | four strains, jpy345t, jpy347, jpy366 and jpy581 were isolated from nitrogen-fixing nodules on the roots of two mimosa spp. (m. cordistipula, m. misera) native to north east brazil, and their taxonomic positions were investigated by a polyphasic approach. all four strains grew at 15-43 °c (optimum, 35 °c), at ph 4.0-7.0 (optimum, ph 5.0) and with 0-2 % nacl (optimum, 0 %). on the basis of 16s rrna gene sequence analysis, a representative strain (jpy345t) showed 97.3 % sequence similarity to t ... | 2011 | 22081715 |
| Enzyme-nanoporous gold biocomposite: excellent biocatalyst with improved biocatalytic performance and stability. | Applications involving biomolecules, such as enzymes, antibodies, and other proteins as well as whole cells, are often hampered by their unstable nature at extremely high temperature and in organic solvents. | 2011 | 21912676 |
| Tsunami lung. | We encountered three cases of lung disorders caused by drowning in the recent large tsunami that struck following the Great East Japan Earthquake. All three were females, and two of them were old elderly. All segments of both lungs were involved in all the three patients, necessitating ICU admission and endotracheal intubation and mechanical ventilation. All three died within 3 weeks. In at least two cases, misswallowing of oil was suspected from the features noted at the time of the detection. ... | 2011 | 22057370 |
| Use of 3-hydroxy fatty acid concentrations in a murine air pouch infection model as a surrogate marker for LPS activity: a feasibility study using environmental Burkholderia cenocepacia isolates. | Using a murine hypodermic air pouch infection model designed to mimic the release of bacterial products at physiological levels, 3-hydroxy fatty acid (3-OH FA) and endotoxin unit levels from Burkholderia cenocepacia isolates were assessed. The B. cenocepacia environmental isolates (n=35) survived in the hypodermic air pouch but did not invade across the peritoneal epithelial layer during a 72-h infection. For all 35 strains, when the molar ratio of C(14:0) 3-OH FA to C(16:0) 3-OH FA in the air p ... | 2011 | 22008505 |
| evaluation of the microbial flora found in woodwind and brass instruments and their potential to transmit diseases. | previous studies of dental devices (toothbrushes, dentures, and protective athletic mouthguards) have demonstrated microbial contamination of these devices and possible transmission of infectious diseases to the users. since woodwind and brass instruments come into intimate contact with the musician's oral cavity and often are passed from student to student without sanitization, the question arises as to whether these instruments are contaminated and can transmit microbial diseases. the purpose ... | 2011 | 21903519 |
| diagnostic use of burkholderia pseudomallei selective media in a low prevalence setting. | routine use of selective media improves diagnosis of burkholderia pseudomallei, but resources may be limited in endemic developing countries. to maximise yield in the relatively low-prevalence setting of kuala lumpur, malaysia, b. pseudomallei selective agar and broth were compared with routine media for 154 respiratory specimens from patients with community-acquired disease. selective media detected three additional culture-positive specimens and one additional melioidosis patient, at a consuma ... | 2011 | 22112687 |
| validation of a predictive survival model in italian patients with cystic fibrosis. | in 2001 liou published a 5-year survival model using cff registry data. | 2011 | 21945182 |
| Identification of Hopanoid Biosynthesis Genes Involved in Polymyxin Resistance in Burkholderia multivorans. | A major challenge to clinical therapy of Burkholderia cepacia complex (Bcc) pulmonary infections is their innate resistance to a broad range of antimicrobials, including polycationic agents such as aminoglycosides, polymyxins, and cationic peptides. To identify genetic loci associated with this phenotype, a transposon mutant library was constructed in B. multivorans ATCC 17616 and screened for increased susceptibility to polymyxin B. Compared to the parent strain, mutant 26D7 exhibited 8- and 16 ... | 2012 | 22006009 |
| Musculoskeletal melioidosis. | Melioidosis is an infectious disease caused by Burkholderia pseudomallei, mostly affecting patients in Southeast Asia and northern Australia. The disease has been increasingly recognized around the world due to the increased levels of travel and population movement. Clinical manifestations of melioidosis range from fulminant septicemic illness to an indolent local infection. The disease often involves multiple organs, including the lung, spleen, liver, and other visceral organs. Musculoskeletal ... | 2011 | 22081283 |
| cloning, purification and crystallographic analysis of a hypothetical protein, bpsl1549, from burkholderia pseudomallei. | burkholderia pseudomallei bpsl1549, a putative protein of unknown function, has been overexpressed in escherichia coli, purified and subsequently crystallized by the hanging-drop vapour-diffusion method using peg as a precipitant to give crystals with overall dimensions of 0.15 × 0.15 × 0.1 mm. native data were collected to 1.47 å resolution at the european synchrotron radiation facility (esrf). the crystals belonged to space group p2(1)2(1)2(1), with unit-cell parameters a = 37.1, b = 45.4, c = ... | 2011 | 22139182 |
| structures of phosphopantetheine adenylyltransferase from burkholderia pseudomallei. | phosphopantetheine adenylyltransferase (ppat) catalyzes the fourth of five steps in the coenzyme a biosynthetic pathway, reversibly transferring an adenylyl group from atp onto 4'-phosphopantetheine to yield dephospho-coenzyme a and pyrophosphate. burkholderia pseudomallei is a soil- and water-borne pathogenic bacterium and the etiologic agent of melioidosis, a potentially fatal systemic disease present in southeast asia. two crystal structures are presented of the ppat from b. pseudomallei with ... | 2011 | 21904046 |
| Burkholderia species are major inhabitants of white lupin cluster roots. | The formation of cluster roots by plants represents a highly efficient strategy for acquisition of sparingly available phosphate. This particular root type is characterized by a densely branched structure and high exudation of organic acids and protons, which are likely to influence the resident bacterial community. Until now, the identity of the bacterial populations living in cluster roots has not been investigated. We applied cultivation-dependent and cultivation-independent methods to charac ... | 2011 | 21908626 |
| Bacterial communities in urban aerosols collected with wetted-wall cyclonic samplers and seasonal fluctuations of live and culturable airborne bacteria. | Airborne transmission of bacterial pathogens from point sources (e.g., ranches, dairy waste treatment facilities) to areas of food production (farms) has been suspected. Determining the incidence, transport and viability of extremely low levels of pathogens require collection of high volumes of air and characterization of live bacteria from aerosols. We monitored the numbers of culturable bacteria in urban aerosols on 21 separate days during a 9 month period using high volume cyclonic samplers a ... | 2011 | 22193549 |
| Structural characterization of two lipopolysaccharide O-antigens produced by the endofungal bacterium Burkholderia sp. HKI-402 (B4). | Two different polysaccharides were isolated and identified from the lipopolysaccharide fraction of endofungal bacterium Burkholderia sp. HKI-402 (B4). The complete structure was elucidated by chemical analysis and 2D NMR spectroscopy as the following: | 2011 | 22115719 |
| genetic relationships among italian and mexican maize-rhizosphere burkholderia cepacia complex (bcc) populations belonging to burkholderia cenocepacia iiib and bcc6 group. | abstract: | 2011 | 21995705 |
| application of a burkholderia cepacia lipase-immobilized silica monolith to batch and continuous biodiesel production with a stoichiometric mixture of methanol and crude jatropha oil. | abstract: | 2011 | 22013896 |
| lipids in cystic fibrosis. | cystic fibrosis is characterized by severe intestinal and pulmonary symptoms, but also changes in the liver, pancreas and reproductive tract. since gastrointestinal symptoms can be controlled, the quality of live and longevity of cystic fibrosis patients is mainly determined by pulmonary inflammation and chronic pulmonary infections with pseudomonas aeruginosa, staphylococcus aureus, burkholderia cepacia and haemophilus influenzae. recent studies developed novel and exciting concepts regarding t ... | 2011 | 21859272 |
| hopanoid production is required for low-ph tolerance, antimicrobial resistance, and motility in burkholderia cenocepacia. | hopanoids are pentacyclic triterpenoids that are thought to be bacterial surrogates for eukaryotic sterols, such as cholesterol, acting to stabilize membranes and to regulate their fluidity and permeability. to date, very few studies have evaluated the role of hopanoids in bacterial physiology. the synthesis of hopanoids depends on the enzyme squalene-hopene cyclase (shc), which converts the linear squalene into the basic hopene structure. deletion of the 2 genes encoding shc enzymes in burkhold ... | 2011 | 21965564 |
| Groundwater seeps facilitate exposure to Burkholderia pseudomallei. | Burkholderia pseudomallei is a saprophytic bacterium which is the causative agent of melioidosis, a common cause of fatal bacterial pneumonia and sepsis in the tropics. The incidence of melioidosis is clustered spatially and temporally and is heavily linked to rainfall and extreme weather events. Clinical case clustering has recently been reported in Townsville, Australia, and has implicated Castle Hill, a granite monolith in the city center, as a potential reservoir of infection. Topsoil and wa ... | 2011 | 21873480 |
| Molecular investigations of a locally acquired case of melioidosis in Southern AZ, USA. | Melioidosis is caused by Burkholderia pseudomallei, a Gram-negative bacillus, primarily found in soils in Southeast Asia and northern Australia. A recent case of melioidosis in non-endemic Arizona was determined to be the result of locally acquired infection, as the patient had no travel history to endemic regions and no previous history of disease. Diagnosis of the case was confirmed through multiple microbiologic and molecular techniques. To enhance the epidemiological analysis, we conducted s ... | 2011 | 22028940 |
| Burkholderia cenocepacia disrupts host cell actin cytoskeleton by inactivating Rac and Cdc42. | Burkholderia cenocepacia, a member of the Burkholderia cepacia complex, is an opportunistic pathogen that causes devastating infections in patients with cystic fibrosis. The ability of B. cenocepacia to survive within host cells could contribute significantly to its virulence in immunocompromised patients. In this study, we explored the mechanisms that enable B. cenocepacia to survive inside macrophages. We found that B. cenocepacia disrupts the actin cytoskeleton of infected macrophages, drast ... | 2011 | 22023324 |
| European Society for Evolutionary Biology meeting. Tracing the common roots of antibiotic resistance. | | 2011 | 21921169 |
| [outbreak of burkholderia cepacia complex caused by contaminated alcohol-free mouthwash]. | a total of three intensive care units (icu) at a german university hospital were involved in an outbreak of burkholderia cepacia complex (bcc). | 2012 | 22273822 |
| evaluation of the environmental specificity of fluorescence in situ hybridization (fish) using fluorescence-activated cell sorting (facs) of probe (pse1284)-positive cells extracted from rhizosphere soil. | we explicitly tested for the first time the 'environmental specificity' of traditional 16s rrna-targeted fluorescence in situ hybridization (fish) through comparison of the bacterial diversity actually targeted in the environment with the diversity that should be exactly targeted (i.e. without mismatches) according to in silico analysis. to do this, we exploited advances in modern flow cytometry that enabled improved detection and therefore sorting of sub-micron-sized particles and used probe ps ... | 2012 | 22264503 |
| heterocyclic urea derivatives and methods of use thereof (wo2010142978). | a series of ureido derivatives incorporating a highly derivatized quinolone scaffold were prepared by a succession of original steps. the lead molecules of these compounds are the quinolone antibacterials of the nalidixic acid/ciprofloxacin type and they target, similar to these clinically used agents, the dna gyrase/topoisomerase iv of pathogenic bacteria. these new quinolones are claimed to act as efficient antibacterials against a large number of drug-resistant bacteria such as staphylococcus ... | 2012 | 22264034 |
| the burkholderia cenocepacia bdsf quorum sensing fatty acid is synthesized by a bifunctional crotonase homologue having both dehydratase and thioesterase activities. | signal molecules of the diffusible signal factor (dsf) family have been shown recently to be involved in regulation of pathogenesis and biofilm formation in diverse gram-negative bacteria. dsf signals are reported to be active not only on their cognate bacteria, but also on unrelated bacteria and the pathogenic yeast, candida albicans. dsfs are monounsaturated fatty acids of medium chain length containing an unusual cis-2 double bond. although genetic analyses had identified genes involved in ds ... | 2012 | 22221091 |
| bacterial taxa associated with the lung lichen lobaria pulmonaria are differentially shaped by geography and habitat. | the correlation between the taxonomic composition of alphaproteobacteria, burkholderia and nitrogen fixers associated with the lichen lobaria pulmonaria and the geographical distribution of the host was studied across four sites in europe. results proved that the diversity of alphaproteobacteria is affected by geography, while those of burkholderia and nitrogen fixers were mostly driven by local habitat. this difference indicates a higher stability of the association between alphaproteobacteria ... | 2012 | 22268428 |
| characterisation of the burkholderia pseudomallei k96243 cps i coding region. | burkholderia pseudomallei is the causative agent of melioidosis, a disease endemic to regions of southeast asia and northern australia. both humans and a range of other animal species are susceptible to melioidosis and the production of a group 3 polysaccharide capsule in b. pseudomallei is essential for virulence. this b. pseudomallei capsular polysaccharide (cps) i comprises unbranched manno-heptopyranose residues and is encoded by a 34.5 kb locus on chromosome 1. despite the importance of thi ... | 2012 | 22252864 |
| the genetic and molecular basis of o-antigenic diversity in burkholderia pseudomallei lipopolysaccharide. | lipopolysaccharide (lps) is one of the most important virulence and antigenic components of burkholderia pseudomallei, the causative agent of melioidosis. lps diversity in b. pseudomallei has been described as typical, atypical or rough, based upon banding patterns on sds-page. here, we studied the genetic and molecular basis of these phenotypic differences. bioinformatics was used to determine the diversity of genes known or predicted to be involved in biosynthesis of the o-antigenic moiety of ... | 2012 | 22235357 |
| burkholderia and cupriavidus spp. are the preferred symbionts of mimosa spp. in southern china. | rhizobia were isolated from invasive mimosa spp. (m. diplotricha and m. pudica) in dehong district of the province of yunnan in subtropical southern china. almost all of the 98 isolates were β-rhizobia in the genera burkholderia and cupriavidus. these strains were analyzed for their distribution characteristics together with strains from a previous study from sishuangbanna. the proportion of nodules containing each β-rhizobial genus varied between mimosa species, with cupriavidus being predomina ... | 2012 | 22268711 |
| structural analysis of capsular polysaccharides expressed by burkholderia mallei and burkholderia pseudomallei. | capsular polysaccharides (cpss) were isolated from o-polysaccharide deficient strains of burkholderia mallei and burkholderia pseudomallei using a modified hot phenol/water extraction procedure. glycosyl composition, methylation, maldi-tof ms analyses as well as (1)h nmr spectroscopy including cosy, tocsy, noesy, hmbc and hsqc experiments identified the presence of two distinct cps antigens in the samples exhibiting the following structures: this study confirms the ability of b. mallei to expres ... | 2011 | 22221792 |
| extractive fermentation for enhanced production of thailandepsin a from burkholderia thailandensis e264 using polyaromatic adsorbent resin diaion hp-20. | thailandepsin a is natural product of burkholderia thailandensis e264 with potent histone deacetylase inhibitory activities and promising anticancer activities. the titer of thailandepsin a is very low (less than 10 mg/l) from limited empirical fermentation. to facilitate preclinical evaluations and potentially clinical development of thailandepsin a, systematic optimization and extractive fermentation of thailandepsin a from b. thailandensis e264 culture in flasks were investigated in this pilo ... | 2012 | 22246221 |
| new beta rhizobial strains able to efficiently nodulate parapiptadenia rigida (benth.) brenan. | among the leguminous trees natives to uruguay parapiptadenia rigida (angico), a mimosoideae legume, is one of the most promising species for agroforestry. like many other legumes, it is able to establish symbiotic associations with rhizobia and belongs to the group known as "nitrogen fixing trees", which are major components of agroforestry systems. information about rhizobial symbionts for this genus is scarce, and thus the aim of this work was to identify and characterize rhizobia associated w ... | 2012 | 22226956 |