| conservation and diversity of seed associated endophytes in zea across boundaries of evolution, ethnography and ecology. | endophytes are non-pathogenic microbes living inside plants. we asked whether endophytic species were conserved in the agriculturally important plant genus zea as it became domesticated from its wild ancestors (teosinte) to modern maize (corn) and moved from mexico to canada. kernels from populations of four different teosintes and 10 different maize varieties were screened for endophytic bacteria by culturing, cloning and dna fingerprinting using terminal restriction fragment length polymorphis ... | 2011 | 21673982 |
| reduced mortality in cystic fibrosis patients treated with tobramycin inhalation solution. | background: though tobramycin inhalation solution has been used for over a decade to improve lung function and reduce exacerbations in patients with cystic fibrosis (cf), its effects on mortality have not been well-described. this study aimed to assess the association between use of tobramycin inhaled solution and mortality in patients with cf and chronic pseudomonas aeruginosa (pa) infection. methods: longitudinal logistic regression was used to assess the association between current-year repor ... | 2011 | 21815282 |
| melioidosis imported from west africa to europe. | abstract. we report the first case of imported melioidosis in spain from a diabetic immigrant who visited west africa during the rainy season. because of the unusual presentation of this disease in africa, clinical and microbiological diagnosis of imported melioidosis from this continent can be very elusive. | 2011 | 21813848 |
| autotransporters and their role in the virulence of burkholderia pseudomallei and burkholderia mallei. | burkholderia pseudomallei and burkholderia mallei are closely related gram-negative bacteria responsible for the infectious diseases melioidosis and glanders, respectively. autotransporters (ats) comprise a large and diverse family of secreted and outer membrane proteins that includes virulence-associated invasins, adhesins, proteases, and actin-nucleating factors. the b. pseudomallei k96243 genome contains 11 predicted ats, eight of which share homologs in the b. mallei atcc 23344 genome. this ... | 2011 | 21811486 |
| genome and characterization of phages bcep22 and bcepil02, founders of a novel phage type in burkholderia cenocepacia. | within the burkholderia cepacia complex (bcc), b. cenocepacia is the most common species associated with aggressive infections in the lungs of cystic fibrosis patients, causing disease that is often refractive to treatment by antibiotics. phage therapy may be a potential alternative form of treatment for these infections. here we describe the genome of the previously described therapeutic b. cenocepacia podophage bcepil02 and its close relative, bcep22. phage bcep22 was found to contain a circul ... | 2011 | 21804006 |
| the burkholderia pseudomallei {delta}asd mutant exhibits attenuated intracellular infectivity and imparts protection against acute inhalation melioidosis in mice. | burkholderia pseudomallei, the cause of serious and life threatening diseases in humans, is of national biodefense concern because of its potential use as a bioterrorism agent. this microbe is listed as a select agent by the cdc, therefore, development of vaccines is of significant importance. here, we further investigated the growth characteristics of a recently created b. pseudomallei 1026b ?asd mutant in vitro, in a cell model, and in an animal model of infection. the mutant was typified by a ... | 2011 | 21807903 |
| extreme antimicrobial peptide and polymyxin b resistance in the genus burkholderia. | cationic antimicrobial peptides and polymyxins are a group of naturally occurring antibiotics that can also possess immunomodulatory activities. they are considered a new source of antibiotics for treating infections by bacteria that are resistant to conventional antibiotics. members of the genus burkholderia, which includes various human pathogens, are inherently resistant to antimicrobial peptides. the resistance is several orders of magnitude higher than that of other gram-negative bacteria s ... | 2011 | 21811491 |
| cable pili and the associated 22 kda adhesin contribute to burkholderia cenocepacia persistence in vivo. | infection by burkholderia cenocepacia in cystic fibrosis (cf) patients is associated with poor clinical prognosis. previously, we demonstrated that one of the highly transmissible strains, bc7, expresses cable pili and the associated 22 kda adhesin, both of which contribute to bc7 binding to airway epithelial cells. however, the contribution of these factors to induce inflammation and bacterial persistence in vivo is not known. | 2011 | 21811611 |
| highly sensitive direct detection and quantification of burkholderia pseudomallei in environmental soil samples using real-time pcr. | the soil bacterium and potential biothreat agent burkholderia pseudomallei causes the infectious disease melioidosis, which is naturally acquired through environmental contact with the bacterium. environmental detection of b. pseudomallei represents the basis for the development of a geographical risk map to humans and livestock. the aim of the present study was to develop a highly sensitive culture-independent dna-based method that allows direct quantification of b. pseudomallei from soil. we e ... | 2011 | 21803915 |
| endovascular therapy for infected aortic aneurysms. | objective: to determine the outcome of endovascular therapy for an infected aortic aneurysm in patients with or without aorto-aerodigestive/aortocaval fistulas. methods: from september 2005 to may 2010, 21 patients, 17 abdominal and four thoracic infected aortic aneurysms were treated with an endovascular stent graft at songklanagarind hospital, thailand. five patients presented with fistula complications, 1 aortoesophageal, 1 aortobronchial, 1 aortocaval, and 2 aortoenteric fistulas. lifelong a ... | 2011 | 21802238 |
| structural and functional analysis of phytotoxin toxoflavin-degrading enzyme. | pathogenic bacteria synthesize and secrete toxic low molecular weight compounds as virulence factors. these microbial toxins play essential roles in the pathogenicity of bacteria in various hosts, and are emerging as targets for antivirulence strategies. toxoflavin, a phytotoxin produced by burkholderia glumae bgr1, has been known to be the key factor in rice grain rot and wilt in many field crops. recently, toxoflavin-degrading enzyme (txde) was identified from paenibacillus polymyxa jh2, there ... | 2011 | 21799856 |
| thailandepsins: bacterial products with potent histone deacetylase inhibitory activities and broad-spectrum antiproliferative activities. | histone deacetylase (hdac) inhibitors have emerged as a new class of anticancer drugs, with one synthetic compound, saha (vorinostat, zolinza; 1), and one natural product, fk228 (depsipeptide, romidepsin, istodax; 2), approved by fda for clinical use. our studies of fk228 biosynthesis in chromobacterium violaceum no. 968 led to the identification of a cryptic biosynthetic gene cluster in the genome of burkholderia thailandensis e264. genome mining and genetic manipulation of this gene cluster fu ... | 2011 | 21793558 |
| improvement of anti-burkholderia mouse monoclonal antibody from various phage-displayed single-chain antibody libraries. | to improve anti-burkholderia monoclonal antibody (mab) binding affinity, six single chain variable fragments (scfvs) constructed previously were used as scaffolds to construct large highly-diversified phage-displayed mouse scfv random and domain libraries. first, we employed random mutagenesis to introduce random point mutations into entire variable regions, generating six random libraries. additionally, the oligonucleotide-directed mutagenesis was targeted on complementarity-determining region ... | 2011 | 21787781 |
| combining vaccination and postexposure cpg therapy provides optimal protection against lethal sepsis in a biodefense model of human melioidosis. | the gram-negative bacterium burkholderia pseudomallei is the causative agent of melioidosis, a major cause of lethal sepsis and morbidity in endemic areas of southeast asia and a potential bioterrorism threat. we have used susceptible balb/c mice to evaluate the potential of targeting vaccination and generic immunotherapy to the lung for optimal protection against respiratory challenge. intranasal vaccination with live attenuated b. pseudomallei increased survival and induced interferon-?-secret ... | 2011 | 21791666 |
| neutrophils are the predominant cell-type to associate with burkholderia pseudomallei in a balb/c mouse model of respiratory melioidosis. | a variety of studies have implicated neutrophils and the rapid induction of cytokine in the host response in melioidosis. here a balb/c mouse model of infection with aerosolised burkholderia pseudomalleik96243 has been used to understand the immune response to infection in this model and verify other infection models that show rapid growth of bacteria, colonisation of tissues and periphery, induction of cytokines and influx of neutrophils. uniquely, this study has also determined the association ... | 2011 | 21798336 |
| burkholderia pseudomallei-induced cell fusion in u937 macrophages can be inhibited by monoclonal antibodies against host cell surface molecules. | burkholderia pseudomallei induces the formation of multinucleated giant cells in cell monolayers. after infection of human macrophage-like u937 cells with b. pseudomallei, addition of monoclonal antibodies against integrin-associated protein (cd47), e-selectin (cd62e), a fusion regulatory protein (cd98), and e-cadherin (cd324) suppressed multinucleated giant cells in a concentration-dependent manner while monoclonal antibodies against other surface molecules did not inhibit fusion despite bindin ... | 2011 | 21784167 |
| antimicrobial activity of cathelicidin peptides against burkholderia pseudomallei, the causative agent of melioidosis. | | 2011 | 21782393 |
| isolation and characterization of a novel virulent podovirus which infects burkholderia pseudomallei. | abstract: burkholderia pseudomallei is a saprophytic soil bacterium and the etiological agent that causes melioidosis. it is naturally resistant to many antibiotics and therefore is difficult to treat. bacteriophages may provide an alternative source of treatment. we have isolated and characterised the bacteriophage phi bp-amp1. the phage is a member of the podoviridae family and has a genome size of ~ 45 kb. molecular data based on the gene which encodes for the phage tail tubular protein sugge ... | 2011 | 21791081 |
| blocking candida albicans biofilm formation by bdsf and trans-bdsf. | candida is an important opportunistic human fungal pathogen. infections caused by c. albicans are related to the formation of a biofilm. the biofilm enhances the resistance of the c. albicans defense system, increases its resistance to antifungal drugs, and induces increased drug tolerance and makes clinical care more challenging. the in vitro activity of bdsf (cis-2-dodecenoic acid) and trans-bdsf (trans-2-dodecenoic acid) against candida albicans growth, germ tube germination and biofilm forma ... | 2011 | 21778264 |
| toll-like receptor 4 region genetic variants are associated with susceptibility to melioidosis. | melioidosis is a tropical infection caused by the gram-negative soil saprophyte burkholderia pseudomallei. despite broad exposure of northeastern thais, disease develops in only a small proportion of individuals. although diabetes is a risk factor, the mechanisms of host susceptibility to melioidosis are still poorly understood. we postulated that toll-like receptors (tlrs) regulate host susceptibility to disease, and that genetic variation in tlrs is associated with melioidosis. we analyzed the ... | 2011 | 21776015 |
| spontaneous and evolutionary changes in the antibiotic resistance of burkholderia cenocepacia observed by global gene expression analysis. | abstract: background: burkholderia cenocepacia is a member of the burkholderia cepacia complex group of bacteria that cause infections in individuals with cystic fibrosis. b. cenocepacia isolate j2315 has been genome sequenced and is representative of a virulent, epidemic cf strain (et12). its genome encodes multiple antimicrobial resistance pathways and it is not known which of these is important for intrinsic or spontaneous resistance. to map these pathways, transcriptomic analysis was perform ... | 2011 | 21781329 |
| an outbreak of burkholderia cepacia complex associated with intrinsically contaminated nasal spray. | objective. to determine the source of burkholderia cepacia complex associated with a hospital outbreak and describe the measures taken to identify and confirm the source. setting. a 250-bed, tertiary care pediatric hospital in denver, colorado. methods. an epidemiologic investigation was used to identify possible causes for an apparent outbreak of b. cepacia complex in pediatric patients who had new positive cultures with this organism from december 2003 to february 2004. chart review, microbiol ... | 2011 | 21768765 |
| in vitro activity of sitafloxacin against burkholderia pseudomallei. | | 2011 | 21775108 |
| multi-functional characteristics of the pseudomonas aeruginosa type iii needle-tip protein, pcrv; comparison to orthologs in other gram-negative bacteria. | pseudomonas aeruginosa possesses a type iii secretion system (t3ss) to intoxicate host cells and evade innate immunity. this virulence-related machinery consists of a molecular syringe and needle assembled on the bacterial surface, which allows delivery of t3 effector proteins into infected cells. to accomplish a one-step effector translocation, a tip protein is required at the top end of the t3 needle structure. strains lacking expression of the functional tip protein fail to intoxicate host ce ... | 2011 | 21772833 |
| caenorhabditis elegans npr-1-mediated behaviors are suppressed in the presence of mucoid bacteria. | caenorhabditis elegans exhibits a diverse range of behaviors in response to bacteria. the presence of bacterial food influences c. elegans aerotaxis, aggregation, locomotion, and pathogen avoidance behaviors through the activity of the npr-1 neuropeptide receptor. here, we show that mucoid strains of bacteria that produce an exopolysaccharide matrix do not induce npr-1-dependent behaviors. in the presence of mucoid strains of bacteria, the c. elegans laboratory wild-type (wt) strain n2 exhibits ... | 2011 | 21768378 |
| survival of burkholderia pseudomallei in distilled water for 16 years. | burkholderia pseudomallei was examined after being maintained in distilled water at 25°c for 16 years. the gram stain was atypical (pale pink cocci or coccobacilli). the estimated number of live and dead b. pseudomallei was 3.8×10(7) cells/ml and 1.4×10(5) cells/ml, respectively. a colony count on agar of 1.0×10(6) cfu/ml suggested that a proportion of cells were in a viable but non-culturable state. colony morphology was different from the parental isolate for 84% of colonies. pulsed-field gel ... | 2011 | 21764093 |
| a role for the burkholderia pseudomallei type three secretion system cluster 1 bpscn gene in virulence. | burkholderia pseudomallei, the causal agent of melioidosis, employs a number of virulence factors during its infection of mammalian cells. one such factor is the type three secretion system (ttss), which is proposed to mediate the transport and secretion of bacterial effector molecules directly into host cells. the b. pseudomallei genome contains three ttss gene clusters (designated ttss1, ttss2 and ttss3). previous research has indicated that neither ttss1 nor ttss2 are involved in b. pseudomal ... | 2011 | 21768285 |
| development of a colorimetric colony-screening assay for detection of defluorination by micro-organisms. | aims: to develop a colorimetric colony-screening assay to facilitate the isolation of micro-organisms capable of defluorination. methods and results: a metal-dye chelate, zirconium-xylenol orange was used to detect fluoride ions released from a fluorinated substrate through microbial metabolism. depolymerised zirconium reagent gave the greatest visual contrast for the presence of fluoride compared to more polymerised forms of zirconium reagent. the sensitivity of the assay was greatest when th ... | 2011 | 21767280 |
| typhoon-related leptospirosis and melioidosis, taiwan, 2009. | to the editor: global climatic changes have resulted in more natural disasters worldwide. these natural disasters can then cause outbreaks of emerging infectious diseases, including leptospirosis and melioidosis (1-7). in 2009, the moderate-strength typhoon morakot, with a maximum cumulative rainfall amount up to 3,059.5 mm, damaged taiwan. after this natural disaster, unusual epidemics of leptospirosis and melioidosis occurred. the main objective of this study was to clarify whether these epide ... | 2011 | 21762606 |
| melioidosis in birds and burkholderia pseudomallei dispersal, australia. | to the editor: melioidosis is an emerging infectious disease of humans and animals caused by the gram-negative bacterium burkholderia pseudomallei, which inhabits soil and surface water in the disease-endemic regions of southeast asia and northern australia (1). the aim of this study was to assess the potential for birds to spread b. pseudomallei. birds are known carriers of various human pathogens, including influenza viruses, west nile virus, campylobacter jejuni, and antimicrobial drug-resist ... | 2011 | 21762599 |
| etymologia: melioidosis. | from the greek melis, distemper of asses, oeide-s, resemblance, and osis, a suffix indicating an abnormal condition or disease. alfred whitmore, a british pathologist serving in burma, and his assistant c. s. krishnaswami first described melioidosis in 1912. the infection became known as whitmore's disease. in 1925, ambrose t. stanton and william fletcher, the researchers who identified burkholderia pseudomallei as the infection's causative agent, renamed the infection melioidosis because of its ... | 2011 | 21762621 |
| melioidosis, phnom penh, cambodia. | we describe 58 adult patients with melioidosis in cambodia (2007-2010). diabetes was the main risk factor (59%); 67% of infections occurred during the rainy season. bloodstream infection was present in 67% of patients, which represents 12% of all bloodstream infections. the case-fatality rate was 52% and associated with inappropriate empiric treatment. | 2011 | 21762590 |
| natural burkholderia mallei infection in dromedary, bahrain. | we confirm a natural infection of dromedaries with glanders. multilocus variable number tandem repeat analysis of a burkholderia mallei strain isolated from a diseased dromedary in bahrain revealed close genetic proximity to strain dubai 7, which caused an outbreak of glanders in horses in the united arab emirates in 2004. | 2011 | 21762586 |
| epidemiology and investigation of melioidosis, southern arizona. | burkholderia pseudomallei is a bacterium endemic to southeast asia and northern australia, but it has not been found to occur endemically in the united states. we report an ostensibly autochthonous case of melioidosis in the united states. despite an extensive investigation, the source of exposure was not identified. | 2011 | 21762589 |
| melioidosis acquired by traveler to nigeria. | we describe melioidosis associated with travel to nigeria in a woman with diabetes, a major predisposing factor for this infection. with the prevalence of diabetes projected to increase dramatically in many developing countries, the global reach of melioidosis may expand. | 2011 | 21762592 |
| phb biosynthesis in catabolite repression mutant of burkholderia sacchari. | due to the effect of catabolite repression, sugar mixtures cannot be metabolized in a rapid and efficient way implicating in lower productivity in bioprocesses using lignocellulosic hydrolysates. in gram-negative bacteria, this mechanism is mediated by the phosphotransferase system (pts), which concomitantly internalizes and phosphorylates sugars. in this study, we isolated a uv mutant of burkholderia sacchari, called lfm828, which transports hexoses and pentoses by a non-pts uptake system. this ... | 2011 | 21761218 |
| a recombinant burkholderia intracellular motility a (bima) protein for immunodiagnosis of glanders. | glanders, caused by the gram-negative, nonmotile bacterium burkholderia mallei, is a contagious and highly fatal disease of equines. during the last decade number of glanders outbreaks has increased steadily. the disease also has high zoonotic significance and b. mallei is listed biological warfare agent. complement fixation test (cft) is a routinely used and internationally recognised test to screen equine sera for the glanders. however, discrepant results have been observed by cft. the low sen ... | 2011 | 21752949 |
| burkholderia pseudomallei in unchlorinated domestic bore water, tropical northern australia. | to determine whether unchlorinated bore water in northern australia contained burkholderia pseudomallei organisms, we sampled 55 bores; 18 (33%) were culture positive. multilocus sequence typing identified 15 sequence types. the b. pseudomallei sequence type from 1 water sample matched a clinical isolate from a resident with melioidosis on the same property. | 2011 | 21762588 |
| in vivo fluorescence imaging of bacteriogenic cyanide in the lungs of live mice infected with cystic fibrosis pathogens. | pseudomonas aeruginosa (pa) and burkholderia cepacia complex (bcc), commonly found in the lungs of cystic fibrosis (cf) patients, often produce cyanide (cn), which inhibits cellular respiration. cn in sputa is a potential biomarker for lung infection by cf pathogens. however, its actual concentration in the infected lungs is unknown. | 2011 | 21750709 |
| infection in immunocompromised hosts and the organ transplant recipient -essentials. | | 2011 | 21748845 |
| diversity analysis of diazotrophic bacteria associated with the roots of tea (camellia sinensis (l.) o. kuntze). | the diversity elucidation by amplified ribosomal dna restriction analysis and 16s rdna sequencing of 96 associative diazotrophs, isolated from the feeder roots of tea on enriched nitrogen-free semisolid media, revealed the predominance of gram-positive over gram-negative bacteria within the kangra valley in himachal pradesh, india. the gram-positive bacteria observed belong to two taxonomic groupings; firmicutes, including the genera bacillus and paenibacillus; and actinobacteria, represented by ... | 2011 | 21715960 |
| the dioxygenase-encoding olsd gene from burkholderia cenocepacia causes the hydroxylation of the amide-linked fatty acyl moiety of ornithine-containing membrane lipids. | burkholderia cenocepacia is an important opportunistic pathogen, and one of the most striking features of the burkholderia genus is the collection of polar lipids present in its membrane, including phosphatidylethanolamine (pe) and ornithine-containing lipids (ols), as well as the 2-hydroxylated derivatives of pe and ols (2-oh-pe and 2-oh-ols, respectively), which differ from the standard versions by virtue of the presence of a hydroxyl group at c2 (2-oh) of an esterified fatty acyl residue. sim ... | 2011 | 21707055 |
| evidence of burkholderia pseudomallei-specific immunity in patient sera persistently nonreactive by the indirect hemagglutination assay. | the indirect hemagglutination assay (iha) is the most frequently used serological test to confirm exposure to burkholderia pseudomallei. patients with culture-confirmed disease often have a nonreactive iha at presentation and occasionally fail to seroconvert on serial testing. we investigated whether using antigens derived from the cultured isolates of persistently iha-nonreactive patient sera improved the sensitivity of the iha. in addition, we assessed the antigen-specific lymphocyte response ... | 2011 | 21677111 |
| biosynthesis of novel polyhydroxyalkanoate containing 3-hydroxy-4-methylvalerate by chromobacterium sp. usm2. | aims:ôçé polyhydroxyalkanoate (pha) with enhanced physicochemical properties will be ideal for a wide range of practical applications. the incorporation of 3-hydroxy-4-methylvalerate (3h4mv) into the polymer backbone is known to improve the overall properties of the resulting polymer. however, the most suitable micro-organism and pha synthase that can synthesize this monomer efficiently still remain unknown at present. therefore, we evaluated the abilities of a locally isolated chromobacterium s ... | 2011 | 21689225 |
| legume-nodulating betaproteobacteria: diversity, host range and future prospects. | rhizobia form specialized nodules on the roots of legumes (fabaceae) and fix nitrogen in exchange for carbon from the host plant. although the majority of legumes form symbioses with rhizobium and its relatives in the alphaproteobacteria, some legumes, such as those in the large genus mimosa, are nodulated predominantly by betaproteobacteria in the genera burkholderia and cupriavidus. the principal centers of diversity of these bacteria are in central brazil and south africa. molecular phylogene ... | 2011 | 21830951 |
| mucoid morphotype variation of burkholderia multivorans during chronic cystic fibrosis lung infection is correlated with changes in metabolism, motility, biofilm formation and virulence. | burkholderia cepacia complex (bcc) bacteria are opportunistic pathogens infecting hosts such as cystic fibrosis (cf) patients. bcc long-term infection of cf patient airways has been associated with emergence of phenotypic variation. here we studied two burkholderia multivorans clonal isolates displaying different morphotypes from a chronically infected cf patient to evaluate traits development during lung infection. expression profiling of mucoid d2095 and nonmucoid d2214 isolates revealed decre ... | 2011 | 21835880 |
| a multiplexed immunoassay system based upon reciprocating centrifugal microfluidics. | a novel, centrifugal disk-based micro-total analysis system (µtas) for low cost and high throughput semi-automated immunoassay processing was developed. a key innovation in the disposable immunoassay disk design is in a fluidic structure that enables very efficient micro-mixing based on a reciprocating mechanism in which centrifugal acceleration acting upon a liquid element first generates and stores pneumatic energy that is then released by a reduction of the centrifugal acceleration, resulting ... | 2011 | 21721711 |
| discovery of a metagenome-derived enzyme that produces branched-chain acyl-(acyl-carrier-protein)s from branched-chain +¦-keto acids. | | 2011 | 21714057 |
| impact of endofungal bacteria on infection biology, food safety, and drug development. | | 2011 | 21738468 |
| [a case of melioidosis occurring after a long-term stay in vietnam that developed pulmonary cavitation and relapsed with multiple pulmonary nodules]. | a 69-year-old man complained of fever in september 2009, after returning from vietnam where he has been working for 20 years. he had diabetes mellitus and was on diabetic oral medication. he was examined at a nearby hospital, and found out to have pneumonia with cavity formation in the right upper lobe which was found out to be not due to tuberculosis. although the patient once recovered with antibacterial medicine, after a few months, in january 2010, he was admitted to our hospital because of ... | 2011 | 21735746 |
| solvent dielectric effect and side chain mutation on the structural stability of burkholderia cepacia lipase active site: a quantum mechanical/molecular mechanics study. | quantum mechanical and molecular dynamics methods were used to analyze the structure and stability of neutral and zwitterionic configurations of the extracted active site sequence from a burkholderia cepacia lipase, histidyl-seryl-glutamin (his86-ser87-gln88) and its mutated form, histidyl-cysteyl-glutamin (his86-cys87-gln88) in vacuum and different solvents. the effects of solvent dielectric constant, explicit and implicit water molecules and side chain mutation on the structure and stability o ... | 2011 | 21710316 |
| low-dose exposure of c57bl/6 mice to burkholderia pseudomallei mimics chronic human melioidosis. | burkholderia pseudomallei is the etiological agent of human melioidosis, a disease with a broad spectrum of clinical manifestations ranging from fatal septicemia to chronic localized infection or asymptomatic latent infection. most clinical and immunological studies to date have focused on the acute disease process; however, little is known about pathology and immune response in chronic melioidosis. here, we have developed a murine model of chronic disease by challenging c57bl/6 mice intranasall ... | 2011 | 21703409 |
| [analysis of an outbreak of burkholderia cepacia for risk stratification]. | the possibility of nosocomial infection is determined by factors relating to the patient, the presence and degree of immunodeficiency, and interventions that become stronger the risk. a nosocomial outbreak is the emergence of an unusual case of a specific disease and is due to an infectious agent only. our objective was to analyze the risk of burkholderia cepacia with a risk stratification criterion in a pediatric intensive care unit (icu). | 2011 | 21703140 |
| burkholderia multivorans acts as an antagonist against the growth of burkholderia pseudomallei in soil. | burkholderia multivorans nki379 has an antagonistic effect against the growth of b. pseudomallei, which we have demonstrated here using agar diffusion tests and a transwell system. bacterial representatives were isolated from agricultural crop soil and mixed to construct a partial bacterial community structure that based on the results of reproducible patterns following pcr-dgge (denaturing gradient gel electrophoresis) analysis from total soil chromosomes. the antagonistic effect of b. multivor ... | 2011 | 21752084 |
| cascade bioreactor with submerged biofilm for aerobic treatment of tunisian landfill leachate. | a bioreactor cascade with a submerged biofilm is proposed to treat young landfill leachate of jbel chakir landfill site south west from capital tunis, tunisia. the prototype was run under different organic loading charges varying from 0.6 to 16.3kgtocm(-3)day(-1). without initial ph adjustment total organic carbon (toc) removal rate varied between 65% and 97%. the total reduction of cod reached 92% at a hydraulic retention time of 36h. however, the removal of total kjeldahl nitrogen for loading ... | 2011 | 21703853 |
| incidence of bacteremic melioidosis in eastern and northeastern thailand. | burkholderia pseudomallei, the causative agent of melioidosis, is endemic in northeastern thailand. population-based disease burden estimates are lacking and limited data on melioidosis exist from other regions of the country. using active, population-based surveillance, we measured the incidence of bacteremic melioidosis in the provinces of sa kaeo (eastern thailand) and nakhon phanom (northeastern thailand) during 2006-2008. the average annual incidence in sa kaeo and nakhon phanom per 100,000 ... | 2011 | 21734135 |
| altered macrophage function is associated with severe burkholderia pseudomallei infection in a murine model of type 2 diabetes. | this study used a murine model of type 2 diabetes (bks.cg-dock7(m) +/+lepr(db)/j mice) to investigate the inflammatory and cellular mechanisms predisposing to burkholderia pseudomallei infection and co-morbid diabetes. homozygous db/db (diabetic) mice developed extreme obesity, dyslipidaemia and glucose intolerance leading to hyperglycaemia and overt type 2 diabetes. compared to their heterozygous db/+ (non-diabetic) littermates, diabetic mice rapidly succumbed to subcutaneous b. pseudomallei in ... | 2011 | 21835260 |
| burkholderia zhejiangensis sp. nov., a novel methyl parathion-degrading bacterium isolated from a wastewater-treating system. | the taxonomic status of a methyl parathion-degrading strain op-1t isolated from a wastewater-treating system was determined. the cells were gram-negative, non-spore-forming, non-motile, and rod-shaped. phylogenetic analysis based on 16s rrna gene sequence indicated that this strain belongs to the genus burkholderia, as it showed highest sequence similarities to burkholderia glathei atcc 29195t (97.4 %), burkholderia sordidicola kctc 12081t (96.5 %) and burkholderia bryophila lmg 23644t (96.3 %). ... | 2011 | 21828021 |
| clinical and economic burden of community-acquired pneumonia amongst adults in the asia-pacific region. | community-acquired pneumonia (cap) is an important cause of mortality and morbidity amongst adults in the asia-pacific region. literature published between 1990 and may 2010 on the clinical and economic burden of cap amongst adults in this region was reviewed. cap is a significant health burden with significant economic impact in this region. chronic obstructive pulmonary disease, cardiovascular disease, diabetes mellitus and advanced age were risk factors for cap. aetiological agents included s ... | 2011 | 21683553 |
| potent antimicrobial peptides with selectivity for bacillus anthracis over human erythrocytes. | in this study, 39 antimicrobial peptides, most with documented low haemolytic activity and potent efficacy against gram-negative and gram-positive bacteria, were evaluated for their haemolytic activity against human red blood cells as well as their antimicrobial activity against escherichia coli, burkholderia thailandensis, bacillus globigii and bacillus anthracis. the majority of the peptides had a minimum inhibitory concentration (mic) of <10++m against b. globigii. however, only eight of thes ... | 2011 | 21741801 |
| effects of trehalose and sorbitol on the activity and structure of pseudomonas cepacia lipase: spectroscopic insight. | the stability of enzymes with no reduction in their catalytic activity still remains a critical issue in industrial applications. naturally occurring osmolytes are commonly used as protein stabilizers. in this study we have investigated the effects of sorbitol and trehalose on the structural stability and activity of pseudomonas cepacia lipase (pcl), using uv-visible, circular dichroism (cd) and fluorescence spectroscopy. surface plasmon resonance (spr) technique was used to trace changes in the ... | 2011 | 21741990 |
| the effects of metabolite molecules produced by drinking water-isolated bacteria on their single and multispecies biofilms. | the elucidation of the mechanisms by which diverse species survive and interact in drinking water (dw) biofilm communities may allow the identification of new biofilm control strategies. the purpose of the present study was to investigate the effects of metabolite molecules produced by bacteria isolated from dw on biofilm formation. six opportunistic bacteria, viz. acinetobacter calcoaceticus, burkholderia cepacia, methylobacterium sp., mycobacterium mucogenicum, sphingomonas capsulata and staph ... | 2011 | 21732713 |
| evaluation and optimisation of bacterial genomic dna extraction for no-culture techniques applied to vinegars. | direct genomic dna extraction from vinegars was set up and suitability for pcr assays performed by pcr/dgge and sequencing of 16s rrna gene. the method was tested on 12 intermediary products of special vinegars, fruit vinegars and condiments produced from different raw materials and procedures. dnas extraction was performed on pellets by chemical, enzymatic, resin mediated methods and their modifications. suitable yield and dna purity were obtained by modification of a method based on the use of ... | 2011 | 21839388 |
| burkholderia pseudomallei: an uncommon cause of bacteraemic pneumonia in a diabetic. | a 55-year-old woman presented with fever, breathlessness and shock. she was diagnosed to have diabetes mellitus (type 2) after admission. blood culture grew burkholderia pseudomallei. the patient responded to intravenous ceftozidime for two weeks and a prolonged course of six months with cotrimoxazole and doxycycline. | 2011 | 21838203 |
| evaluation of surrogate animal models of melioidosis. | burkholderia pseudomallei is the gram-negative bacterial pathogen responsible for the disease melioidosis. b. pseudomallei establishes disease in susceptible individuals through multiple routes of infection, all of which may proceed to a septicemic disease associated with a high mortality rate. b. pseudomallei opportunistically infects humans and a wide range of animals directly from the environment, and modeling of experimental melioidosis has been conducted in numerous biologically relevant mo ... | 2010 | 21772830 |
| akt-mediated proinflammatory response of mononuclear phagocytes infected with burkholderia cenocepacia occurs by a novel gsk3+¦-dependent, i+¦b kinase-independent mechanism. | the environmental bacterium burkholderia cenocepacia causes opportunistic lung infections in immunocompromised individuals, particularly in patients with cystic fibrosis. infections in these patients are associated with exacerbated inflammation leading to rapid decay of lung function, and in some cases resulting in cepacia syndrome, which is characterized by a fatal acute necrotizing pneumonia and sepsis. b. cenocepacia can survive intracellularly in macrophages by altering the maturation of the ... | 2011 | 21697459 |
| bioluminescent diagnostic imaging to characterize altered respiratory tract colonization by the burkholderia pseudomallei capsule mutant. | pneumonia is a common manifestation of the potentially fatal disease melioidosis, caused by the select agent bacteria burkholderia pseudomallei. in this study we describe a new model system to investigate pulmonary melioidosis in vivo using bioluminescent-engineered bacteria in a murine respiratory disease model. studies were performed to validate that the stable, light producing b. pseudomallei strain jw280 constitutively produced light in biologically relevant host-pathogen interactions. hairl ... | 2011 | 21720539 |
| burkholderia cenocepacia shvr-regulated genes that influence colony morphology, biofilm formation, and virulence. | burkholderia cenocepacia is an opportunistic pathogen that primarily infects cystic fibrosis (cf) patients. previously, we reported that shvr, a lysr regulator, influences colony morphology, virulence, and biofilm formation and regulates the expression of an adjacent 24-kb genomic region encoding 24 genes. in this study, we report the functional characterization of selected genes in this region. a tn5 mutant with shiny colony morphology was identified with a polar mutation in bcas0208, predicted ... | 2011 | 21690240 |
| knockout and pullout recombineering for naturally transformable burkholderia thailandensis and burkholderia pseudomallei. | phage ++-red proteins are powerful tools for pulling and knocking out chromosomal fragments but have been limited to the +¦-proteobacteria. procedures are described here to easily knock out (ko) and pull out (po) chromosomal dna fragments from naturally transformable burkholderia thailandensis and burkholderia pseudomallei. this system takes advantage of published compliant counterselectable and selectable markers (sacb, phes, gat and the arabinose-utilization operon) and ++-red mutant proteins. ... | 2011 | 21738123 |
| resistance of the burkholderia cepacia complex to fosmidomycin and fosmidomycin derivatives. | the burkholderia cepacia complex (bcc) is a group of 17 closely related opportunistic pathogens that are able to infect the respiratory tract of cystic fibrosis patients. bcc bacteria are intrinsically resistant to many antibiotics and are therefore difficult to eradicate. fosmidomycin could be a new therapeutic agent to treat bcc infections as it inhibits 1-deoxy-d-xylulose-5-phosphate reductoisomerase (dxr), a key enzyme in the non-mevalonate pathway essential in bcc bacteria for isoprenoid sy ... | 2011 | 21724375 |
| molecular characterization of clinical burkholderia pseudomallei isolates from india. | multilocus sequence typing of seven isolates of burkholderia pseudomallei from india showed considerable diversity, with six different sequence types. possible dissemination of melioidosis by historical trading routes is supported by links to strains from southeast asia, china, and africa and the presence of the burkholderia mallei allele of the bima gene. | 2011 | 21734136 |
| sponge-associated microbial antarctic communities exhibiting antimicrobial activity against burkholderia cepacia complex bacteria. | the aerobic heterotrophic bacterial communities isolated from three different antarctic sponge species were analyzed for their ability to produce antimicrobial compounds active toward cystic fibrosis opportunistic pathogens belonging to the burkholderia cepacia complex (bcc). the phylogenetic analysis performed on the 16s rrna genes affiliated the 140 bacterial strains analyzed to 15 genera. just three of them (psychrobacter, pseudoalteromonas and arthrobacter) were shared by the three sponges. ... | 2011 | 21742025 |
| dissection of the burkholderia intracellular life cycle using a photothermal nanoblade. | burkholderia pseudomallei and burkholderia thailandensis are related pathogens that invade a variety of cell types, replicate in the cytoplasm, and spread to nearby cells. we have investigated temporal and spatial requirements for virulence determinants in the intracellular life cycle, using genetic dissection and photothermal nanoblade delivery, which allows efficient placement of bacterium-sized cargo into the cytoplasm of mammalian cells. the conserved bsa type iii secretion system (t3ss(bsa) ... | 2011 | 21730143 |
| [melioidosis: a poorly known tropical disease]. | a 35 year-old man was admitted to the hospital for fever upon returning from the caribbean area. he died 48 hours later, after developing pulmonary lesions that were complicated by multi-organ failure, despite rapid diagnosis of melioidosis by mass spectrometry on blood cultures. melioidosis is a rare bacterial disease in the traveller that is caused by burkholderia pseudomallei. although the clinical presentation is variable, pneumonia is the most frequent finding. diagnosis may be considered i ... | 2011 | 21692313 |
| distinct carbohydrate and lipid-based molecular patterns within lipopolysaccharides from burkholderia cepacia contribute to defense-associated differential gene expression in arabidopsis thaliana. | lipopolysaccharides are structural components within the cell walls of gram-negative bacteria. the lpss as microbe-associated molecular pattern (mamp) molecules can trigger defense-related responses involved in mamp-triggered immunity and basal resistance in plants, presumably from an initial perception event. lps from burkholderia cepacia as well as two fragments, the glycolipid, lipid a and the polysaccharide (ops-core) chain, were used to treat arabidopsis thaliana seedlings to evaluate the e ... | 2011 | 21733976 |
| genetic and biochemical map for the biosynthesis of occidiofungin, an antifungal produced by burkholderia contaminans strain ms14. | a striking feature of burkholderia contaminans strain ms14 is the production of a glycolipopeptide named occidiofungin. occidiofungin has a broad range of antifungal activities against plant and animal pathogens. in this study, a complete covalent structure characterization and identification of the whole genomic dna region for the occidiofungin gene (ocf) cluster are described. discovery of the presence of 2,4-diaminobutyric acid and 3-chloro-+¦-hydroxytyrosine and elucidation of the structure ... | 2011 | 21742901 |
| alanine racemase mutants of burkholderia pseudomallei and burkholderia mallei and use of alanine racemase as a non-antibiotic-based selectable marker. | burkholderia pseudomallei and burkholderia mallei are category b select agents and must be studied under bsl3 containment in the united states. they are typically resistant to multiple antibiotics, and the antibiotics used to treat b. pseudomallei or b. mallei infections may not be used as selective agents with the corresponding burkholderia species. here, we investigated alanine racemase deficient mutants of b. pseudomallei and b. mallei for development of non-antibiotic-based genetic selection ... | 2011 | 21720554 |
| colonization of the gut with gram negative bacilli, its association with neonatal sepsis and its clinical relevance in a developing country. | this study examines the pattern of colonization of the neonatal gut by aerobic gram negative bacilli (gnb) and evaluates the association between gut colonization and sepsis in the developing world. this deserves attention because of the high incidence of sepsis and the differences in hygienic environment in developing countries compared to the developed world. the study was carried out on neonates in a tertiary care government hospital. the serial gut samples were analyzed (gastric aspirate, ga- ... | 2011 | 21719575 |
| crystal structures of dntr inducer binding domains in complex with salicylate offer insights into the activation of lysr-type transcriptional regulators. | activation of lysr-type transcription factors (lttrs) is thought to result from conformational changes that occur when inducer molecules bind to their inducer binding domains (ibds). however, the exact nature of these changes remains to be fully elucidated. we present the crystal structures of two truncated constructs of the lttr dntr in their apo- forms and in complex with its natural inducer molecule, salicylate. these provide a fuller picture of the conformational changes that can occur in lt ... | 2011 | 21692874 |
| burkholderia pseudomallei infection in a child with cystic fibrosis: acquisition in the western hemisphere. | melioidosis, an infection caused by the bacterium burkholderia pseudomallei, is endemic to southeast asia and northern australia but is only very rarely seen in patients in the united states. we report pulmonary b pseudomallei infection in a young girl with cystic fibrosis (cf) who had never traveled to asia or australia. biochemical and epidemiologic investigation determined aruba as the likely site of disease acquisition. this report highlights the ability of patients with cf to acquire this o ... | 2011 | 21729895 |
| acute melioid community-acquired pneumonia. | to better understand the characteristics of patients with acute melioid community-acquired pneumonia (cap) on emergency department (ed) arrival, and the risk factors in patients with acute melioid cap that differ from those in patients with severe cap of causes other than melioidosis. | 2011 | 21696989 |
| Liver disease in adult patients with cystic fibrosis: A frequent and independent prognostic factor associated with death or lung transplantation. | BACKGROUND & AIMS: Increased life expectancy in patients with cystic fibrosis (CF) allows better knowledge of non-pulmonary complications like liver disease (CFLD). However, few data have been published in large adult cohorts. The aim of this study was to estimate the prevalence and the prognosis of CFLD in adult CF patients. METHODS: A retrospective analysis of a monocentric cohort of adult CF patients prospectively followed, at least every year, was performed. CFLD was diagnosed using publishe ... | 2011 | 21703187 |
| a novel fk-506-binding-like protein that lacks peptidyl-prolyl isomerase activity is involved in intracellular infection and in vivo virulence of burkholderia pseudomallei. | burkholderia pseudomallei is a facultative intracellular bacterial pathogen causing melioidosis, an often fatal infectious disease that is endemic in several tropical and subtropical areas around the world. we previously described a ptk2 cell-based plaque assay screening system of b. pseudomallei transposon mutants that led to the identification of several novel virulence determinants. using this approach we identified a mutant with reduced plaque formation in which the bpsl0918 gene was disrupt ... | 2011 | 21680634 |
| a naturally derived outer-membrane vesicle vaccine protects against lethal pulmonary burkholderia pseudomallei infection. | burkholderia pseudomallei, and other members of the burkholderia, are among the most antibiotic-resistant bacterial species encountered in human infection. mortality rates associated with severe b. pseudomallei infection approach 50% despite therapeutic treatment. a protective vaccine against b. pseudomallei would dramatically reduce morbidity and mortality in endemic areas and provide a safeguard for the u.s. and other countries against biological attack with this organism. in this study, we in ... | 2011 | 21871517 |
| production of polyhydroxyalkanoates by burkholderia cepacia atcc 17759 using a detoxified sugar maple hemicellulosic hydrolysate. | sugar maple hemicellulosic hydrolysate containing 71.9 g/l of xylose was used as an inexpensive feedstock to produce polyhydroxyalkanoates (phas) by burkholderia cepacia atcc 17759. several inhibitory compounds present in wood hydrolysate were analyzed for effects on cell growth and pha production with strong inhibition observed at concentrations of 1 g/l furfural, 2 g/l vanillin, 7 g/l levulinic acid, and 1 m acetic acid. gradual catabolism of lower concentrations of these inhibitors was observ ... | 2011 | 21953365 |
| comparing the chlorine disinfection of detached biofilm clusters with those of sessile biofilms and planktonic cells in single- and dual-species cultures. | although the detachment of cells from biofilms is of fundamental importance to the dissemination of organisms in both public health and clinical settings, the disinfection efficacies of commonly used biocides on detached biofilm particles have not been investigated. therefore, the question arises whether cells in detached aggregates can be killed with disinfectant concentrations sufficient to inactivate planktonic cells. burkholderia cepacia and pseudomonas aeruginosa were grown in standardized ... | 2011 | 21856824 |
| activation of mmp-9 by human lung epithelial cells in response to cystic fibrosis associated pathogen burkholderia cenocepacia reduced wound healing in vitro. | burkholderia cepacia complex is a group of bacterial pathogens which cause opportunistic infections in cystic fibrosis (cf). the most virulent of these is burkholderia cenocepacia. matrix metalloproteases (mmps) are upregulated in cf patients. the aim of this work was to examine the role of mmps in the pathogenesis of b. cepacia complex, which has not been explored to date. real-time pcr analysis showed that b. cenocepacia infection up-regulated mmp-2 and mmp-9 genes in cf lung cell line, cfbe41 ... | 2011 | 21743026 |
| small-molecule inhibitor binding to an n-acyl-homoserine lactone synthase. | quorum sensing (qs) controls certain behaviors of bacteria in response to population density. in gram-negative bacteria, qs is often mediated by n-acyl-l-homoserine lactones (acyl-hsls). because qs influences the virulence of many pathogenic bacteria, synthetic inhibitors of acyl-hsl synthases might be useful therapeutically for controlling pathogens. however, rational design of a potent qs antagonist has been thwarted by the lack of information concerning the binding interactions between acyl-h ... | 2011 | 21730159 |
| molecular investigations of pena-mediated ß-lactam resistance in burkholderia pseudomallei. | burkholderia pseudomallei is the etiological agent of melioidosis. because of the bacterium's intrinsic resistance and propensity to establish latent infections, melioidosis therapy is complicated and prolonged. newer generation ß-lactams, specifically ceftazidime, are used for acute phase therapy, but resistance to this cephalosporin has been observed. the chromosomally encoded pena gene encodes a putative twin arginine translocase (tat)-secreted ß-lactamase, and pena mutations have been implic ... | 2011 | 21747814 |
| the type vi secretion system of burkholderia cenocepacia affects multiple rho family gtpases disrupting the actin cytoskeleton and the assembly of nadph oxidase complex in macrophages. | burkholderia cenocepacia is a gram-negative opportunistic pathogen of patients with cystic fibrosis and chronic granulomatous disease. the bacterium survives intracellularly in macrophages within a membrane-bound vacuole (bccv) that precludes the fusion with lysosomes. the underlying cellular mechanisms and bacterial molecules mediating these phenotypes are unknown. here, we show that intracellular b. cenocepacia expressing a type vi secretion system (t6ss) affects the activation of the rac1 and ... | 2011 | 22023353 |
| Synthesis of the trisaccharide outer core fragment of Burkholderia cepacia pv. vietnamiensis lipooligosaccharide. | The synthesis of ß-Gal-(1?3)-a-GalNAc-(1?3)-ß-GalNAc allyl trisaccharide as the outer core fragment of Burkholderia cepacia pv. vietnamiensis lipooligosaccharide was accomplished through a concise, optimized, multi-step synthesis, having as key steps three glycosylations, that were in-depth studied performing them under several conditions. The target trisaccharide was designed with an allyl aglycone in order to open a future access to the conjugation with an immunogenic protein en route to the d ... | 2011 | 22209378 |
| [Superior vena cava obstruction without symptoms?]. | | 2011 | 21943542 |
| Study of the effect of DNA polymorphisms in the mannose-binding lectin gene (MBL2) on disease severity in Slovak cystic fibrosis patients. | Lung infections are the leading cause of morbidity and mortality in cystic fibrosis (CF). Mannose-binding lectin (MBL) is a key factor in innate immunity. We therefore investigated whether MBL2 gene variants are associated with pulmonary function or susceptibility to Pseudomonas aeruginosa and Burkholderia cepacia infection in Slovak patients affected with CF. DNA polymorphisms in exon 1 and the promoter region were typed by single base primer extension assay in 91 patients and 100 healthy contr ... | 2011 | 22131319 |
| biodegradation analyses of trichloroethylene (tce) by bacteria and its use for biosensing of tce. | trichloroethylene (tce) is a toxic, recalcitrant groundwater pollutant. tce-degrading microorganisms were isolated from various environments. the aerobic bacteria isolated from toluene- and tryptophan-containing media were pseudomonas sp. strain asa86 and burkholderia sp. strain tam17, respectively; these are necessary for inducing tce biodegradation in a selective medium. the half-degradation time of tce to a concentration of 1mg/l was 18 h for strain asa86 and 7 days for strain tam17. while id ... | 2011 | 21872018 |
| insertion sequences evolutionary patterns highlight convergent genetic inactivations and recent genomic islands acquisitions among epidemic burkholderia cenocepacia. | the burkholderia cenocepacia b&b clone was previously found responsible of an epidemic outbreak within an intensive care unit in france. this clone belongs to the st32 clonal complex which is one of the most prevalent among french cystic fibrosis patients, and is known to be related to the highly virulent et12 clonal complex. genomic repartition biases of insertion sequences (is) were investigated to improve our understanding of the evolutionary events leading to b. cenocepacia diversification a ... | 2011 | 21980044 |
| photodynamic antimicrobial chemotherapy (pact) in combination with antibiotics for treatment of burkholderia cepacia complex infection. | this study aimed to determine if photodynamic antimicrobial chemotherapy (pact) was effective in the treatment of burkholderia cepacia complex infection and whether a synergistic effect was evident if pact was used in combination with antibiotics. the susceptibility of both planktonic and biofilm cultures of b. cepacia complex strains to methylene blue (mb) and meso-tetra(n-methyl-4-pyridyl)porphine tetra-tosylate (tmp)-mediated pact was determined alone and in combination with antibiotics used ... | 2012 | 22079165 |
| A Burkholderia pseudomallei toxin inhibits helicase activity of translation factor eIF4A. | The structure of BPSL1549, a protein of unknown function from Burkholderia pseudomallei, reveals a similarity to Escherichia coli cytotoxic necrotizing factor 1. We found that BPSL1549 acted as a potent cytotoxin against eukaryotic cells and was lethal when administered to mice. Expression levels of bpsl1549 correlate with conditions expected to promote or suppress pathogenicity. BPSL1549 promotes deamidation of glutamine-339 of the translation initiation factor eIF4A, abolishing its helicase ac ... | 2011 | 22076380 |
| Theoretical study of the mechanism of proton transfer in the esterase EstB from Burkholderia gladioli. | Esterase EstB from Burkholderia gladioli belongs to a novel class of esterases homologous to penicillin binding proteins, notably DD-peptidase and class C ß-lactamases. It can cleave the side chain acetyl ester group from cephalosporins leaving the ß-lactam ring intact, which is a feature of relevance to industrial biocatalytic applications in the production of semisynthetic cephalosporin derivatives. Due to its important role as a potential biocatalyst in industry, the significance of EstB has ... | 2011 | 21910435 |
| characterization of rhamnolipid production by burkholderia glumae. | to investigate if burkholderia glumae can produce rhamnolipids, define a culture medium for good production yields, analyse their composition and determine their tensioactive properties. | 2011 | 21933203 |
| Histone deacetylase inhibitors from Burkholderia thailandensis. | Bioactivity-guided fractionation of an extract of Burkholderia thailandensis led to the isolation and identification of a new cytotoxic depsipeptide and its dimer. Both compounds potently inhibited the function of histone deacetylases 1 and 4. The monomer, spiruchostatin C (2), was tested side by side with the clinical depsipeptide FK228 (1, Istodax, romidepsin) in a murine hollow fiber assay consisting of 12 implanted tumor cell lines. Spiruchostatin C (2) showed good activity toward LOX IMVI ... | 2011 | 21967146 |