| [antimicrobial activities of norfloxacin against clinical isolates from ocular infections]. | in order to evaluate antimicrobial activity of norfloxacin (nflx), minimum inhibitory concentration (mics) of nflx and control drugs were determined against clinical isolates from ocular infections that were obtained in our laboratory from july, 1993 to december, 1994. the results are summarized as follows; 1. compared to mic distributions of nflx against clinical isolates from ocular infections studied in 1986 and 1987, the mic80 of nflx against corynebacterium spp., enterobacter spp., serratia ... | 1995 | 7474324 |
| [frequency of clinical isolation of glucose non-fermentative gram-negative rods and their susceptibilities to antibacterial agents]. | a comparison was made for frequencies of isolation o glucose non-fermentative gram-negative rods ((g)nf-gnr) from clinical specimens during a period from july, 1986 to june, 1987 (the first period) and that from january, 1994 to december, 1994 (the second period). also, minimum inhibitory concentrations of principal drugs were determined against these isolates. the obtained results are summarized as follows: 1. thirty four (34) species of (g)nf-gnr were found from 35,200 clinical specimens in th ... | 1995 | 7474336 |
| a physical genome map of the burkholderia cepacia type strain. | burkholderia cepacia (basonym pseudomonas cepacia), the type species of the new genus burkholderia, is of interest, not only because of its broad catabolic capacity and its ability to antagonize soil-borne plant pathogens, but also because of its causative role in infections in man, which are particularly evident in patients with cystic fibrosis. a physical map of the 8.1 mb genome of the b. cepacia type-strain atcc 25416 was constructed by applying two-dimensional pulsed-field gel electrophores ... | 1995 | 7476209 |
| detection of pseudomonas (burkholderia) cepacia using pcr. | pseudomonas cepacia colonization of the lung is associated with increased morbidity and mortality for cystic fibrosis (cf) patients. the lack of a sensitive detection method for pseudomonas cepacia in cf sputum has resulted in controversy regarding its epidemiology. we designed a pcr method to detect p. cepacia using p. cepacia 16 s rrna sequences as the amplification target region. the pcr amplification with purified dna as template yielded the expected 209-bp products from p. cepacia, but not ... | 1995 | 7478781 |
| [infections of central venous catheters]. | the central venous catheter is considered the lifeline of patients requiring long-term parenteral nutrition, and its infectious complications accordingly represent a frequent cause for hospitalization and morbidity. modern catheters made of silastic increase the duration and efficiency of venous access, but their substitution is also more complex and expensive. in a small series of patients undergoing home parenteral nutrition due to short bowel syndrome, and carrying. silastic catheters, infect ... | 1995 | 7481456 |
| substrate specificities of bacterial polyhydroxyalkanoate depolymerases and lipases: bacterial lipases hydrolyze poly(omega-hydroxyalkanoates). | the substrate specificities of extracellular lipases purified from bacillus subtilis, pseudomonas aeruginosa, pseudomonas alcaligenes, pseudomonas fluorescens, and burkholderia cepacia (former pseudomonas cepacia) and of extracellular polyhydroxyalkanoate (pha) depolymerases purified from comamonas sp., pseudomonas lemoignei, and p. fluorescens gk13, as well as that of an esterase purified from p. fluorescens gk 13, to various polyesters and to lipase substrates were analyzed. all lipases and th ... | 1995 | 7487042 |
| burkholderia cepacia and delta f508 homozygosity in cystic fibrosis. | | 1995 | 7492181 |
| analysis of strains of burkholderia (pseudomonas) cepacia isolated in a nosocomial outbreak by biochemical and genomic typing. | we analyzed strains of burkholderia (formerly pseudomonas) cepacia isolated in a nosocomial outbreak by biochemical and genomic typing methods. one hundred isolates of b. cepacia were obtained from patients at several wards in a single hospital from march 1983 to february 1984. these isolates were classified into 12 groups by a new biochemical typing scheme on the basis of the production of six enzymes and the production of hemolytic substance and yellow pigment. among them, 33 strains collected ... | 1995 | 7494027 |
| characterization of a lipopolysaccharide o antigen containing two different trisaccharide repeating units from burkholderia cepacia serotype e (o2). | the o antigen of the lipopolysaccharide of burkholderia cepacia serotype e (o2) was shown by a combination of methylation analysis, partial hydrolysis, nmr, and mass spectrometric methods to be a high molecular weight polysaccharide composed of two different trisaccharide repeating units in the ratio 2:1. the major trisaccharide component is composed of two alpha-d-mannopyranosyl and one beta-d-galactopyranosyl residues with the structure, [-->2)-alpha-d-man p-(1-->2)-alpha-d-man p-(1-->4)-beta- ... | 1995 | 7497480 |
| [cystic fibrosis and burkholderia (formerly pseudomonas) cepacia: an increasing clinical and psychosocial problem]. | | 1995 | 7501071 |
| iron-regulated salicylate synthesis by pseudomonas spp. | two iron-regulated compounds have been found in acidified ethyl acetate extracts from culture supernatants of pseudomonas aeruginosa and pseudomonas cepacia type-strains. synthesis of both compounds paralleled iron-deficient growth, and was repressed in the presence of 100 microm-fecl3. yields of these substances varied among different strains and attained maximum levels during stationary phase. thin layer chromatographic analysis in five different solvent systems revealed that the slower-moving ... | 1993 | 7504066 |
| isolation of pseudomonas cepacia in cystic fibrosis patient. | pulmonary infection on cystic fibrosis (cf) patients are associated with a limited qualitative number of microorganisms. during the colonization process, staphylococcus aureus usually precedes pseudomonas aeruginosa. this latter is at first non-mucoid, being replaced or associated to a mucoid morphotype which is rare in other diseases. in 1980, pseudomonas cepacia appeared as an important agent in cf pulmonary infections with a mean frequency of about 6.1% isolations in different parts of the wo ... | 1993 | 7504157 |
| pilot study of exposure to pseudomonas pseudomallei in northern vietnam. | | 1993 | 7504340 |
| arbitrarily primed polymerase chain reaction as a rapid method to differentiate crossed from independent pseudomonas cepacia infections in cystic fibrosis patients. | we used dna fingerprinting by the arbitrarily primed polymerase chain reaction (ap-pcr) technique for an epidemiological investigation of 23 pseudomonas cepacia isolates obtained from 11 cystic fibrosis (cf) patients attending our cf center. this approach was compared with ribotyping, pulsed-field gel electrophoresis (pfge), and conventional phenotypic typing. ap-pcr and ribotyping were identical in resolving power, since the two methods generated four different profiles and identified the same ... | 1993 | 7504684 |
| epidemic of pseudomonas cepacia in an adult cystic fibrosis unit: evidence of person-to-person transmission. | an epidemic of pseudomonas cepacia occurred in an adult cystic fibrosis center in the united kingdom, despite a policy of segregation of infected and noninfected patients within the hospital. investigation of the outbreak by ribotyping and pulsed-field gel electrophoresis to characterize p. cepacia strain genomes together with inquiry into social contacts between patients revealed evidence of person-to-person transmission outside the hospital environment. segregation policies aimed at reducing t ... | 1993 | 7505294 |
| nosocomial outbreak of pseudomonas cepacia respiratory infection in immunocompromised patients associated with contaminated nebulizer devices. | from may 1990 to august 1991, 36 patients admitted to the department of internal medicine in a medical school hospital with hematological malignancies or solid tumors, developed respiratory tract colonization with pseudomonas cepacia. sixteen (44.4%) of these patients developed pneumonia, and four (11.1%) died of respiratory failure due to p. cepacia pneumonia. extensive survey of the hospital environment as well as equipment showed that nebulizer devices used by the patients for inhalation were ... | 1993 | 7505795 |
| immunological studies of an artificial antigen with specificity of a common polysaccharide antigen of pseudomonas aeruginosa. | synthetic d-rhamnan, with the structure of pseudomonas aeruginosa common polysaccharide antigen (cpa), was conjugated with bsa. the artificial antigen obtained, and the natural antigens, lipopolysaccharides (lps) of p. aeruginosa and pseudomonas cerasi with rhamnan chains of the same structure, were studied by elisa with rabbit antibodies to the d-rhamnan-bsa conjugate and to the p. cerasi o-antigen. immunological relations between the lps of p. aeruginosa and p. cerasi determined by cpa as well ... | 1993 | 7506094 |
| construction and application of chromosomally integrated lac-lux gene markers to monitor the fate of a 2,4-dichlorophenoxyacetic acid-degrading bacterium in contaminated soils. | a reporter gene system, containing luxab and laczy, was constructed and integrated, using tn7 transposition, into the chromosome of a 2,4-dichlorophenoxyacetic acid (2,4-d)-degrading soil bacterium, pseudomonas cepacia (bri6001), to monitor its fate when introduced into soil microcosms. the genes were stably maintained in the modified strain of bri6001, bri6001l, for more than 300 generations in the absence of selection pressure, and had no apparent effects on biochemical or physiological proper ... | 1993 | 7506623 |
| virulence and susceptibility to phagocytosis of pseudomonas pseudomallei r- and s-forms for ground squirrels (citellus citellus l.). | virulence and susceptibility to phagocytosis of pseudomonas pseudomallei r- and s-forms for ground squirrels were studied. the alveolar macrophages and blood leucocytes (polymorphonuclear and mononuclear) was used. s-forms survived and multiplied intracellularly more actively in alveolar macrophages than r-forms and their virulence was considerably higher. | 1993 | 7506861 |
| [epidemiological study on nosocomial infection of pseudomonas cepacia by plasmid analyses]. | in order to clarify the usefulness of plasmid analyses in epidemiological study, plasmid dna profiles, restriction fragment patterns of chromosomal dna analysed by pulsed-field gel electrophoresis (pfge), and patterns of extracellular products were performed in twenty-four nosocomial strains of pseudomonas cepacia isolated at the kagawa medical school hospital. fifteen strains were obtained from 15 patients admitted in a ward, three strains were obtained from nebulizer devices used in a ward, 5 ... | 1993 | 7507505 |
| burkholderia pseudomallei and melioidosis: be aware in temperate area. | | 1993 | 7507550 |
| chronic granulomatous disease presenting in childhood with pseudomonas cepacia septicaemia. | two children who presented with fever, enlarged liver and spleen and ascites were found to have pseudomonas cepacia septicaemia which proved fatal despite appropriate antibiotics and maximum supportive care. chronic granulomatous disease of childhood (cgd) was subsequently diagnosed in both children. the possibility of cgd needs to be considered in any child with unexplained p. cepacia infection. | 1993 | 7508484 |
| identification and characterization of a new plasmid carrying genes for degradation of 2,4-dichlorophenoxyacetate from pseudomonas cepacia csv90. | pseudomonas cepacia csv90 is able to utilize 2,4-dichlorophenoxyacetate (2,4-d) and 2-methyl-4-chlorophenoxyacetate as sole sources of carbon and energy. mutants of the strain csv90 which had lost this ability appeared spontaneously on a nonselective medium. the wild-type strain harbored a 90-kb plasmid, pmab1, whereas 2,4-d-negative mutants either lost the plasmid or had a 70-kb plasmid, pmab2. the plasmid pmab2 was found to have undergone a deletion of a 20-kb fragment of pmab1. the plasmid-fr ... | 1994 | 7509586 |
| structure of the o9 antigen from burkholderia (pseudomonas) cepacia. | the o9 antigen of burkholderia (pseudomonas) cepacia has the following disaccharide repeating-unit: -->4)-alpha-d-glcp-(1-->3)-alpha-l-rhap-(1--> the same unit is present in the o antigens of serratia marcescens strain s1254 and serogroup o4 (predominantly acetylated at o-2 of rhamnose in the latter case). | 1994 | 7511122 |
| physiological aspects of disinfection resistance in pseudomonas cepacia. | a pseudomonas cepacia population was isolated which had reduced susceptibility to iodine and maintained resistance when subcultured several times in phosphate buffer. this population was also resistant to iodine after growth in a minimal medium containing glycerol but not glucose. addition of camp to glucose-grown cells caused increased resistance to iodine. iodine-resistant cultures also demonstrated reduced susceptibility to chlorination but not to heat or metals (cu/ag). the results indicate ... | 1994 | 7511576 |
| effect of sub-mic antibiotics on the cell surface and extracellular virulence determinants of pseudomonas cepacia. | the effects of sub-mics of ciprofloxacin and tobramycin on the cell surface characteristics and extracellular virulence factors of pseudomonas cepacia were evaluated. cells were grown in batch culture under iron-deficient and iron-replete conditions. at sub-mic levels that did not affect bacterial growth cell surface hydrophobicity decreased under both iron-replete and iron-depleted conditions with ciprofloxacin, but increased with tobramycin under iron-sufficient conditions. exopolysaccharide s ... | 1994 | 7511577 |
| susceptibility of pseudomonas pseudomallei to some newer beta-lactam antibiotics and antibiotic combinations using time-kill studies. | | 1994 | 7512547 |
| the 1990-1991 outbreak of melioidosis in the northern territory of australia: epidemiology and environmental studies. | from november 1990 to june 1991 33 acute cases of melioidosis occurred in the northern territory, australia; 25 cases were reported in the capital city, darwin. we carried out an epidemiological investigation to exclude a common source outbreak, describe the risk factors for disease, and develop and institute appropriate control measures. we compared population based attack rates among various risk groups using logistic regression, and the demographic, medical and behavioral risk factors for mel ... | 1993 | 7512752 |
| development of a pcr probe test for identifying pseudomonas aeruginosa and pseudomonas (burkholderia) cepacia. | to develop a system of species specific polymerase chain reaction (pcr) and dna hybridisation based on 16s ribosomal rna sequences for the identification of pseudomonas aeruginosa and pseudomonas (burkholderia) cepacia in sputum from children with cystic fibrosis. | 1994 | 7512994 |
| lung transplantation for mechanically ventilated patients. | as lung transplantation has become more successful, the selection criteria have broadened; however, some relative contraindications to lung transplantation are controversial. some programs consider mechanical ventilation to be a major contraindication to lung transplantation because airway colonization with bacteria may lead to nosocomial infection and respiratory muscle deconditioning may necessitate prolonged postoperative ventilatory support. we report our experience of seven double lung tran ... | 1994 | 7513185 |
| isolation and characterization of pseudomonas pseudomallei flagellin proteins. | flagellin proteins from several different strains of pseudomonas pseudomallei have been isolated and purified to homogeneity by mechanical shearing and differential centrifugation techniques. analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis yielded flagellin monomer protein bands with an estimated m(r) of 43,400. no lipopolysaccharide contamination of the purified protein preparations was detectable by silver staining of flagellin displayed on polyacrylamide gels and by west ... | 1994 | 7513308 |
| virulence factors of burkholderia cepacia. | | 1994 | 7513590 |
| acquisition of pseudomonas cepacia at summer camps for patients with cystic fibrosis. summer camp study group. | to assess the risk of acquisition of pseudomonas cepacia by person-to-person transmission at cystic fibrosis summer camps, we conducted in 1990 a study at three camps attended by patients with cystic fibrosis who had p. cepacia infection and patients without p. cepacia infection but who were considered susceptible to infection. we obtained sputum or throat cultures from campers on their arrival at, weekly during, at the end of, and 14 to 30 days after camp. we compared the incidence of sputum co ... | 1994 | 7513755 |
| the potential of nosocomial transmission of pseudomonas cepacia exists at cardiopulmonary transplant centers. | | 1994 | 7514120 |
| pseudomonas cepacia. | | 1994 | 7514392 |
| effect of ph and co2 on in vitro susceptibility of pseudomonas cepacia to beta-lactams. | inhibition of pseudomonas cepacia (but not pseudomonas aeruginosa) by beta-lactams was decreased in 5% co2 in air compared with air alone. the effect of co2 and ph (range, 6.0 to 8.0) on beta-lactam susceptibility, beta-lactamase expression, and outer membrane proteins was studied in isolates recovered from the sputum of children with cystic fibrosis. incubation in 5% co2 decreased the activity of piperacillin, piperacillin/tazobactam, and ceftazidime, although isolates were still clinically sen ... | 1994 | 7514780 |
| analysis of the pena gene of pseudomonas cepacia 249. | | 1994 | 7514860 |
| pseudomonas cepacia empyema necessitatis after lung transplantation in two patients with cystic fibrosis. | lung transplantation is an accepted modality for patients with cystic fibrosis (cf) who have end-stage respiratory failure. the postoperative course of these patients is often complicated by serious infections with organisms such as pseudomonas aeruginosa and pseudomonas cepacia that may be multiply resistant to conventional antimicrobial agents. we describe two patients with cf who, after double lung transplantation, developed the unusual complication of empyema and empyema necessitatis due to ... | 1994 | 7515778 |
| polysaccharide production in pseudomonas cepacia. | the effects of glucose, osmolarity, temperature and mode of growth on exopolysaccharide production in pseudomonas cepacia was studied in batch culture using a chemically defined growth medium. polymer production was maximal under conditions of a 2% (w/v) glucose supplement, 0.4 m nacl and an incubation temperature of 35 degrees c. in addition, polysaccharide composition and molecular weight varied with mode of growth. on agar culture there was a decrease in pyruvate and rhamnose content yet an i ... | 1994 | 7515963 |
| infectious complications of lung transplantation. impact of cystic fibrosis. | it has been suggested that the presence of airway pathogens prior to lung transplantation (lt) in patients with cystic fibrosis (cf) may place these patients at a higher risk for infectious complications after lt. there is particular concern regarding patients colonized with multiresistant pseudomonas, including p. cepacia, and fungi, including aspergillus. we report our experience with lt for patients with cf and compare the results with those of patients with lt for other indications. between ... | 1994 | 7516251 |
| neutralizing monoclonal antibodies to an extracellular pseudomonas cepacia protease. | pseudomonas cepacia produces at least two extracellular proteases with apparent molecular masses of 36,000 and 40,000 da. the 36-kda protease has high proteolytic activity and the 40-kda protease has low proteolytic activity with hide powder azure as a substrate. monoclonal antibodies (mabs) were raised against the purified 36- and 40-kda proteases. several mabs directed against the 36-kda protease were found to recognize the 40-kda protease by western immunoblot analysis. similarly, a mab direc ... | 1994 | 7516312 |
| isolation and characterization of the outer-membrane proteins of burkholderia (pseudomonas) pseudomallei. | membranes obtained from whole-cell lysates of burkholderia (pseudomonas) pseudomallei (strain 319a) were separated into four fractions by sucrose density gradient centrifugation. membranes were characterized by enzymic and chemical analyses, and by sds-page. cytoplasmic membranes and two forms of outer membranes (om-1, om-2) were detected. the major outer-membrane proteins had m(r) values of 70,000, 38,000, 31,000, 24,000 and 17,000. to determine which outer-membrane proteins were common to b. p ... | 1994 | 7516793 |
| multiple replicons constituting the genome of pseudomonas cepacia 17616. | macrorestriction fragment analysis of dna from pseudomonas cepacia 17616, in conjunction with southern hybridization experiments using junction fragments containing rare restriction enzyme sites as probes, indicated that this bacterium contains three large circular replicons of 3.4, 2.5, and 0.9 megabases (mb). inclusion of the 170-kb cryptic plasmid present in this strain gave an overall estimate of genome size of 7 mb. other southern hybridization experiments indicated that the three large rep ... | 1994 | 7517389 |
| phenotypic comparison between rhizosphere and clinical isolates of burkholderia cepacia. | the phenotypic characteristics of four burkholderia cepacia strains isolated from the rhizosphere and the clinical environment were compared. tests included optimum growth temperature, utilization of carbon sources, production of hcn, indole-3-acetic acid (iaa) and siderophores, proteolytic activity, nitrogen fixation, inhibition of some phytopathogenic fungi, adherence to human mucosal and plant root epithelia, and greenhouse-based plant-growth promotion experiments using cucumber (cucumis sati ... | 1994 | 7517765 |
| linkage analysis of geographic and clinical clusters in pseudomonas cepacia infections by multilocus enzyme electrophoresis and ribotyping. | multilocus enzyme electrophoresis and ribotyping were used to characterize 83 strains of pseudomonas cepacia, mostly isolated from cystic fibrosis (cf) patients, although a number of isolates from non-cf nosocomial infections and reference environmental strains were represented. twenty enzyme electrophoretic types (ets) were determined; of these, one clone (et12) was associated with six of nine ribotypes (rts) said to be geographically representative of the united kingdom and all of the ontario ... | 1994 | 7517953 |
| construction of a specific dna probe for diagnosis of melioidosis and use as an epidemiological marker of pseudomonas pseudomallei. | pseudomonas pseudomallei is a causative agent of melioidosis. the disease manifestations range from fulminant sepsis to asymptomatic seroconversion. in septicemic cases, a mortality rate of 80-90% is reported. rapid and specific diagnosis has become important to the clinical microbiology laboratory. we have developed a p. pseudomallei-specific dna probe. the cloned fragment, herein designated pkku-s23l, contained 1.5 kb of p. pseudomallei chromosomal dna. a radioactively labelled pkku-s23l inser ... | 1994 | 7518036 |
| characteristics of is401, a new member of the is3 family implicated in plasmid rearrangements in pseudomonas cepacia. | we have determined the nucleotide sequence of is401, an insertion sequence implicated in rearrangements of a 170-kb cryptic plasmid from pseudomonas cepacia. our analysis focused on a 4066-bp plasmid fragment containing adjacent copies of is401 and of is408, an element reported previously to activate gene expression in p. cepacia. one objective was to determine if an apparent increase in the copy number of is401 in strains carrying adjacent plasmid copies of these two elements might be due to re ... | 1994 | 7518087 |
| melioidosis survey in kenya. | | 1994 | 7518617 |
| possible nosocomial transmission of pseudomonas cepacia in patients with cystic fibrosis. | to determine whether nosocomial transmission of pseudomonas cepacia occurred at a hospital with endemic p cepacia infection of patients with cystic fibrosis. | 1994 | 7519103 |
| application of pyrolysis mass spectroscopy and sds-page in the study of the epidemiology of pseudomonas cepacia in cystic fibrosis. | representative isolates of pseudomonas cepacia from 15 cystic fibrosis (cf) patients attending the respiratory unit of alder hey childrens' hospital were investigated by sds-page of whole-cell polypeptides and by pyrolysis mass spectroscopy (pms). sds-page was less discriminatory than pms. eleven isolates were indistinguishable by pms and considered to represent re-isolates of an endemic strain; four isolates were distinct from this group, and from one another. p. cepacia was first isolated on t ... | 1994 | 7519269 |
| detection of pseudomonas pseudomallei by pcr and hybridization. | a molecular method for the detection of pseudomonas pseudomallei was developed on the basis of the differences in the 23s rrna sequences of related species of the genus pseudomonas. an 18-base oligonucleotide probe, designed following partial sequencing of 23s ribosomal dna (rdna), was used for the identification and detection of p. pseudomallei either by hybridization or by direct pcr. optimal detection was obtained by hybridization of the probe with pcr-amplified rdna rather than with total ge ... | 1994 | 7519629 |
| characterization of hemolytic and antifungal substance, cepalycin, from pseudomonas cepacia. | hemolytic and antifungal substances, cepalycin i and cepalycin ii, have been isolated from pseudomonas cepacia jn106. a large amount of cepalycins were produced by growing the cells on 1% glycerin-nutrient agar medium covered with a cellophane membrane. the cell-washed supernatant was applied to an amberlite xad2 column, and cepalycins were eluted with 70% ethanol containing 1mm hcl. cepalycins were separated by reverse phase hplc in two fractions which were designated as cepalycin i and cepalyc ... | 1994 | 7519715 |
| post-transplantation survival of cystic fibrosis patients infected with pseudomonas cepacia. | | 1994 | 7520109 |
| serum sensitivity of burkholderia (pseudomonas) cepacia isolates from patients with cystic fibrosis. | bacterial strains which are sensitive to the bactericidal activity of serum are generally considered to be less virulent than serum-resistant strains and are seldom associated with bacteraemia. burkholderia (pseudomonas) cepacia is an important pathogen in cystic fibrosis and is associated with rapid fatal pulmonary decline and bacteraemia in 20% of colonised patients. in this study 19 isolates of b. cepacia expressing either rough or smooth lps were investigated to determine the degree of serum ... | 1994 | 7520313 |
| pseudomonas cepacia in cystic fibrosis. | | 1994 | 7520606 |
| application of indirect immunofluorescence microscopy to colony identification of pseudomonas pseudomallei. | indirect immunofluorescence microscopy was used as a colony identification method of pseudomonas pseudomallei isolates. the antisera against lipopolysaccharide and protein fractions of p. pseudomallei were prepared in guinea pigs and rabbits. with these antisera and fluorescence-labelled anti-guinea pig igg and anti-rabbit igg prepared in sheep (goat), indirect immunofluorescence microscopy was conducted on the colonies of p. pseudomallei and other species of bacteria. the overall results indica ... | 1993 | 7521644 |
| enzymic acylation of methyl d- and l-glycopyranosides: influence of the 3-hydroxyl group. | porcine pancreatic (ppl), candida cylindracea (ccl) and pseudomonas cepacia (lps) lipases, suspended in organic solvents, were used to regioselectively acylate methyl 6-o-butyryl-alpha-d- and l-allopyranosides and methyl 6-o-butyryl-3-deoxy-alpha-d- and l-ribo-hexopyranosides. both the d- and the l-3-deoxy sugars showed a complete regioselectivity, while the reactions of the allosides proved to be less regioselective. this indicates that the presence of the hydroxyl group at c-3 is an unfavourab ... | 1993 | 7521747 |
| melioidosis in a diabetic sailor. | melioidosis was diagnosed in a diabetic sailor who presented with a history and chest radiograph that suggested tuberculosis. melioidosis is a tropical disease with protean manifestations: from asymptomatic infection to chronic cavitary lung disease to overwhelming sepsis. the diagnosis is easily made, even in nonendemic areas when duly considered by the clinicians and microbiology laboratory. ceftazidime has dramatically improved outcomes in hospitalized patients with severe melioidosis. | 1994 | 7521816 |
| transmissibility of pseudomonas cepacia infection in clinic patients and lung-transplant recipients with cystic fibrosis. | in patients with cystic fibrosis, infection with pseudomonas cepacia is associated with poor outcomes. however, the extent of person-to-person transmission and the source of p. cepacia infection after lung transplantation are not well defined. using dna-based typing systems, we sought to determine the genetic relatedness of p. cepacia infection at one cystic fibrosis center. | 1994 | 7521938 |
| formation of chlorocatechol meta cleavage products by a pseudomonad during metabolism of monochlorobiphenyls. | pseudomonas cepacia p166 was able to metabolize all monochlorobiphenyls to the respective chlorobenzoates. although they transiently accumulated, the chlorobenzoate degradation intermediates were further metabolized to chlorocatechols, which in turn were meta cleaved. 2- and 3-chlorobiphenyl both produced 3-chlorocatechol, which was transformed to an acyl halide upon meta cleavage. 3-chlorocatechol metabolism was toxic to the cells and impeded monochlorobiphenyl metabolism. in the case of 2-chlo ... | 1994 | 7521996 |
| inhibition of fungal growth by pseudomonas aeruginosa and pseudomonas cepacia isolated from patients with cystic fibrosis. | this study was undertaken because of the infrequency of infections due to candida species in patients with cystic fibrosis despite their extensive treatment with broad-spectrum antibiotics. in vitro susceptibility studies revealed significant inhibition of 11 strains of fungi known to infect human beings by 10 strains of pseudomonas aeruginosa and nine strains of pseudomonas cepacia isolated from the sputum of patients with cystic fibrosis. the fungi were candida krusei, candida keyfr, candida g ... | 1994 | 7522262 |
| in vitro suppression of pseudomonas cepacia after limited exposure to subinhibitory concentrations of amiloride and 5-(n,n-hexamethylene) amiloride. | amiloride (a) for aerosolized therapy in cystic fibrosis (cf) is under investigation. antipseudomonal properties of a and a analogs in vitro have recently been described. this study was performed to determine if there was suppression of p. cepacia growth after a limited 2-hour exposure to (subinhibitory) concentrations of a +/- tobramycin (t) or to the analog 5-(n,n-hexamethylene) amiloride (hma) in vitro. the mic of each drug against five different p. cepacia strains was determined. cells were ... | 1994 | 7522319 |
| effect of antibiotic treatment on inflammatory markers and lung function in cystic fibrosis patients with pseudomonas cepacia. | the acquisition of pseudomonas cepacia in patients with cystic fibrosis is associated with increasing deterioration in lung function and more frequent hospital admissions. pseudomonas cepacia is usually resistant to several antibiotics in vitro, but the response of patients colonised with the organism has not been extensively studied in vivo. | 1994 | 7522353 |
| reaction of phthalate dioxygenase reductase with nadh and nad: kinetic and spectral characterization of intermediates. | phthalate dioxygenase reductase (pdr) is an electron transferase that contains fmn, which accepts a hydride from nadh, and a [2fe-2s] center, which transfers electrons to phthalate dioxygenase. the reduction of pdr by nadh has been studied by stopped-flow spectroscopy. data from studies using both portio- and deuterio-nadh were analyzed by nonlinear curve fitting and numerical simulation techniques. the results of these analyses indicate that the reductive half-reaction of pdr consists of five d ... | 1994 | 7522555 |
| lipase of pseudomonas cepacia for biotechnological purposes: purification, crystallization and characterization. | commercial lipase (triacylglycerol lipase, ec 3.1.1.3) of pseudomonas cepacia (amano) has been purified to homogeneity by a single chromatography on phenyl sepharose. the eluted lipase crystallized spontaneously at 4 degrees c in the eluent, containing 58-69% 2-propanol. the yield of the lipase was 87-100% and the specific activity during the hydrolysis of triolein 5800 u/mg protein. this protein has a molecular weight of 34.1 kda as analyzed by sodium dodecyl sulfate-polyacrylamide gel electrop ... | 1994 | 7522571 |
| pseudomonas pseudomallei isolates collected over 25 years from a non-tropical endemic focus show clonality on the basis of ribotyping. | between 1966 and 1991, melioidosis, a disease caused by pseudomonas pseudomallei that is mostly confined to tropical regions, occurred in farm animals and a farmer in temperate south-west western australia. using an escherichia coli probe containing a ribosomal rna operon, p. pseudomallei dna from isolates from 8 animals, a soil sample and the human case showed an identical ribotype on southern blotting. the ribotype was different from the 3 commonest ribotypes seen in tropical australia. this m ... | 1994 | 7523158 |
| pseudomonas cepacia of the temporal bone: malignant external otitis in a patient with cystic fibrosis. | | 1994 | 7524007 |
| [the immunodiagnosis of melioidosis by a solid-phase variant of radioimmunological analysis (ria)]. | a variant of the solid-phase radioimmunoassay (ria) has been developed for the detection of specific immunoglobulins in laboratory animals infected with pseudomonas pseudomallei. the proposed method has advantages over the indirect hemagglutination test and the enzyme immunoassay in its sensitivity and specificity. the newly developed ria variant, based on group-specific antigens 6 + d, makes it possible to classify the strain causing the disease with the asian or australian serovar of p. pseudo ... | 1994 | 7524250 |
| pyogenic psoas muscle abscess: report of three cases. | we report three cases of primary psoas muscle abscess. two of these cases were treated by open drainage through a lumbar incision and the other was by ultrasonography (usg)-guided percutaneous aspiration of the abscess. antibiotic therapy was also instituted in all cases. post-operatively, the patients recovered well with no sign of recurrence. usg-guided percutaneous drainage combined with appropriate antibiotic therapy appears to be the first choice procedure for treatment of the primary psoas ... | 1994 | 7524289 |
| degradation of 2,4-dichlorophenoxyacetic acid by pseudomonas cepacia dbo1(pro101) in a dual-substrate chemostat. | to determine the effect of a secondary carbon source on biodegradation of a chloroaromatic compound, pseudomonas cepacia dbo1(pro101) was grown in continuous cultures on basal salts media containing various mixtures of 2,4-dichlorophenoxyacetic acid (2,4-d) and succinate. both succinate and 2,4-d were metabolized over the entire range of dilution rates and compositions analyzed (0.05 to 0.6 h-1). 2,4-dichlorophenol (dcp), the only intermediate detected, accumulated to significant amounts (10 to ... | 1994 | 7524443 |
| cometabolic degradation of trichloroethylene by pseudomonas cepacia g4 in a chemostat with toluene as the primary substrate. | pseudomonas cepacia g4 is capable of cometabolic degradation of trichloroethylene (tce) if the organism is grown on certain aromatic compounds. to obtain more insight into the kinetics of tce degradation and the effect of tce transformation products, we have investigated the simultaneous conversion of toluene and tce in steady-state continuous culture. the organism was grown in a chemostat with toluene as the carbon and energy source at a range of volumetric tce loading rates, up to 330 mumol/li ... | 1994 | 7524444 |
| melioidosis. another etiology of granulomatous osteomyelitis. report of 2 cases. | two cases of granulomatous osteomyelitis caused by melioidosis are reported. histologically, the granulomatous lesions were indistinguishable from tuberculosis and required confirmation with microbiological culture. it is important that melioidosis be considered in the differential diagnosis of granulomatous osteomyelitis since the treatment is quite different from tuberculosis. | 1994 | 7525133 |
| interaction of pseudomonas pseudomallei with macrophages. | | 1994 | 7525379 |
| fatal melioidosis in a tourist returning from thailand. | severe infections with pseudomonas pseudomallei, the causative agent of melioidosis, is a common cause of community acquired septicaemia in south east asia and parts of northern australia, but infection in travellers returning to the united kingdom is extremely rare. we describe the case of a tourist who acquired melioidosis in thailand. despite intensive intravenous therapy with antibiotics to which the organism was sensitive, the patient's infection proved fatal. ps. pseudomallei was isolated ... | 1994 | 7525733 |
| recent advances in biomedical sciences of burkholderia pseudomallei (basonym: pseudomonas pseudomallei). | | 1994 | 7526019 |
| epidemiology of pseudomonas cepacia colonization among patients with cystic fibrosis. | colonization with pseudmonas cepacia in patients with cystic fibrosis (cf) has been associated with increased morbidity and early death, compared with colonization by p. aeruginosa. the mode of acquisition of p. cepacia is not fully understood, although person-to-person spread appears likely. recent epidemiologic studies support the importance of social contact in the spread of p. cepacia among patients with cf. this study was undertaken to investigate the epidemiology of p. cepacia colonization ... | 1994 | 7526323 |
| inapparent transmission of pseudomonas (burkholderia) cepacia among patients with cystic fibrosis. | pseudomonas cepacia is a significant pathogen in children and young adults with cystic fibrosis, and prevention of its acquisition has become an important goal in patient management. although it is now clear that this bacterium can be transmitted from person to person, the frequency of this mode of acquisition and the measures required to prevent it are controversial. in this report we describe the use of a novel genotyping method to extend our previous investigation of person to person transmis ... | 1994 | 7526328 |
| molecular typing of pseudomonas pseudomallei from imported primates in britain. | | 1994 | 7526531 |
| intraspecific metabolic diversity among strains of burkholderia cepacia isolated from decayed onions, soils, and the clinical environment. | a collection of 218 strains of burkholderia cepacia (including 18% strain replicates) was assembled from organic soils, decayed onions, and clinical sources. each strain was characterized for virulence to onion, catabolic ability using the biolog gn microtiter plate, and several other behaviors. overall test reproducibility was estimated at 98%. the results obtained using the biolog gn system corresponded well to those obtained using standard methods. three coefficients of resemblance (gower sim ... | 1994 | 7526797 |
| identification of a meta-cleavage pathway for metabolism of phenoxyacetic acid and phenol in pseudomonas cepacia ac1100. | pseudomonas cepacia strain ac1100 grows luxuriantly on 2,4,5-trichlorophenoxyacetic acid (2,4,5-t) but does not utilize phenoxyacetic acid. after long-term selective pressure on phenoxyacetic acid, mutants designated as strain paa, capable of utilizing phenoxyacetic acid as well as phenol, emerged spontaneously at a frequency of 1 x 10(-8). a deletion mutant strain pt88, which is devoid of a part of 2,4,5-t metabolic pathway, generated neither phenoxyacetic acid utilizing nor phenol-utilizing mu ... | 1994 | 7526858 |
| outbreak of plague-like illness caused by pseudomonas pseudomallei in maharashtra, india. | | 1994 | 7527114 |
| detection of pseudomonas pseudomallei antigen in urine for the diagnosis of melioidosis. | an enzyme-linked immunosorbent assay using a fluorescein isothiocyanate (fitc)-anti-fitc amplification system, has been developed to detect pseudomonas pseudomallei antigen in urine. the assay was evaluated in 135 patients with acute melioidosis, 194 hospitalized patients with other disorders, and 40 healthy controls. antigen was detected in the urine of 123 (91%) patients with melioidosis. urinary antigen was found in 85 (96%) of 89 patients with septicemic melioidosis, all six patients with p. ... | 1994 | 7527187 |
| isolation and partial purification of a carbapenem-hydrolysing metallo-beta-lactamase from pseudomonas cepacia. | a metallo-beta-lactamase has been isolated from a clinical strain of pseudomonas cepacia and partially purified using cibacron blue f3ga coupled agarose. the resulting preparation showed a single band of beta-lactamase activity (pi 8.45) after analytical isoelectric focusing. the enzyme was particularly effective in the hydrolysis of imipenem. meropenem, biapenem, cephaloridine, ceftazidime, benzylpenicillin, ampicillin and carbenicillin were also hydrolysed, although at a lower rate. an unusual ... | 1994 | 7527361 |
| [identification of the bacterium pseudomonas mallei using pseudomonas pseudomallei bacteriophages]. | phage production by pseudomonas pseudomallei and pseudomonas mallei strains has been studied. 32 p. pseudomallei bacteriophages have been isolated. their spectrum of lytic action against p. pseudomallei, p. mallei and other pseudomonas sp. has been defined. it has been shown that p. pseudomallei bacteriophages pp19, pp23, pp33 may be used for identification p. mallei among related pseudomonas. | 1994 | 7527479 |
| pseudomonas cepacia in the sputum of cystic fibrosis patients. | colonization of sputum by pseudomonas cepacia has been associated with progressive respiratory deterioration and a worsening prognosis for some cystic fibrosis patients. after laboratory methods were changed and a selective medium introduced, p. cepacia was isolated from the sputa of 16 out of 82 patients with cystic fibrosis attending this hospital: a prevalence rate of 20%. p. cepacia was found in the first sputum examined of 12 patients after the new methods were introduced, indicating that c ... | 1994 | 7527513 |
| nucleotide sequence and functional analysis of the genes encoding 2,4,5-trichlorophenoxyacetic acid oxygenase in pseudomonas cepacia ac1100. | pseudomonas cepacia ac1100 is able to use the chlorinated aromatic compound 2,4,5-trichlorophenoxyacetic acid (2,4,5-t) as the sole source of carbon and energy. one of the early steps in this pathway is the conversion of 2,4,5-t to 2,4,5-trichlorophenol (2,4,5-tcp). 2,4,5-tcp accumulates in the culture medium when ac1100 is grown in the presence of 2,4,5-t. a dna region from the ac1100 genome has been subcloned as a 2.7-kb ssti-xbai dna fragment, which on transfer to pseudomonas aeruginosa pao1 ... | 1994 | 7527626 |
| infection with pseudomonas cepacia in chronic granulomatous disease: role of nonoxidative killing by neutrophils in host defense. | pseudomonas aeruginosa and pseudomonas cepacia are catalase-producing bacteria, but only p. cepacia causes infections in patients with chronic granulomatous disease (cgd). the in vitro killing of p. aeruginosa and p. cepacia by polymorphonuclear leukocytes (pmnl) from patients with cgd and from healthy adults was assessed. of 6 patients with cgd who developed severe infections with p. cepacia, 4 died. pmnl from the 2 survivors and 6 other patients with cgd killed p. aeruginosa strains efficientl ... | 1994 | 7527826 |
| cloning of the hsp70 (dnak) genes from rhizobium meliloti and pseudomonas cepacia: phylogenetic analyses of mitochondrial origin based on a highly conserved protein sequence. | the genes for hsp70 (or dnak) have been cloned and sequenced from rhizobium meliloti and pseudomonas cepacia, two bacterial species belonging to the alpha- and beta-subdivisions of gram-negative proteobacteria, respectively. on the basis of global alignment of hsp70 proteins, several sequence signatures have been identified that are distinctive of mitochondrial homologs and gram-negative proteobacteria on the one hand and the chloroplasts and cyanobacteria on the other. detailed phylogenetic ana ... | 1994 | 7528198 |
| purification and characterization of a protease from pseudomonas pseudomallei. | pseudomonas pseudomallei is the causative agent of melioidosis, a glanders-like disease of humans and animals. the pathogenesis of melioidosis is not well understood, and the role of various extracellular enzymes produced by p. pseudomallei in the development of this disease is not known. the present studies were designed to purify and characterize an extracellular protease produced by p. pseudomallei isolates and to test the hypothesis that this protease may play a role in melioidosis. the prot ... | 1994 | 7528633 |
| chaperone-mediated activation in vivo of a pseudomonas cepacia lipase. | an extracellular pseudomonas cepacia lipase, lipa, is inactive when expressed in the absence of the product of the lima gene. evidence has been presented that lima is a molecular chaperone. the lipa and lima genes have been cloned in separate and independently inducible expression systems in escherichia coli. these systems were used to test the molecular chaperone hypothesis by investigating whether lima could activate presynthesized prelipase and whether presynthesized lima could activate newly ... | 1994 | 7528875 |
| metabolism of polychlorinated phenols by pseudomonas cepacia ac1100: determination of the first two steps and specific inhibitory effect of methimazole. | resting cells of 2,4,5-trichlorophenoxyacetic acid-grown pseudomonas cepacia ac1100 metabolize both dichlorophenols, such as 2,4-dichlorophenol, 2,6-dichlorophenol, 3,4-dichlorophenol, and 3,5-dichlorophenol, and more highly substituted phenols, such as 2,4,6-trichlorophenol and pentachlorophenol, to the corresponding chlorohydroquinones. the first hydroxylation occurs in the para position of the phenol regardless of whether this position is replaced by a chlorine substituent. the first evidence ... | 1995 | 7529225 |
| characterization of pcr-ribotyping for burkholderia (pseudomonas) cepacia. | ribotyping, a method of genotyping bacterial isolates for epidemiologic study, uses rrna as a probe to detect chromosomal restriction fragment length polymorphisms. although ribotyping is accurate, its utility is limited by the labor and time necessary for southern blot analysis. pcr-ribotyping uses pcr to amplify the 16s-23s intergenic spacer region of the bacterial rrna operon. length heterogeneity in the spacer region has previously been found to be useful as an alternative to standard riboty ... | 1994 | 7529239 |
| plague in india. | | 1995 | 7529862 |
| effects of tunicamycin on the ph-activity pattern of acid phosphatase in pseudomonas pseudomallei. | the liquid culture of pseudomonas pseudomallei shows a complex feature in in the ph-activity pattern of acid phosphatase, not a single peak curve. there was an evident tendency that the higher activity shifted to the higher ph range with the growth of culture. the culture in the presence of tunicamycin (20 micrograms/ml) showed a decreased activity selectively in the higher ph range, while the activity in the lower ph was more heat-labile. the bacterial cells grown on agar plates containing tuni ... | 1994 | 7529943 |
| [antigenic bacterial polysaccharides. structure of o-specific polysaccharides from pseudomonas cepacia serogroups c, i, o1, and o4]. | like some pseudomonas cepacia serogroups studied earlier, serogroups c, i (nakamura), o1 and o4 (heidt) are characterized by the presence of at least two structurally different o-antigenic polysaccharide chains in cell-wall lipopolysaccharides. on the basis of acid hydrolysis, methylation, 1h- and 13c-nmr spectroscopy, including computer-assisted 13c-nmr-based analysis, the complete structures of the predominant polysaccharides of serogroups i (i), c and o4 (iii) and the minor polysaccharides of ... | 1994 | 7530009 |
| multi-resistance isolates possessing characteristics of both burkholderia (pseudomonas) cepacia and burkholderia gladioli from patients with cystic fibrosis. | multi-resistant strains from three uk centres, previously identified as burkholderia (formerly pseudomonas) cepacia, and associated with morbidity, mortality and transmission among patients with cystic fibrosis have been further characterised. biochemical tests and fatty acid analyses indicate these strains to possess some characteristics atypical of b. cepacia but bearing close resemblance to burkholderia gladioli, an organism previously regarded solely as a plant pathogen and a hindrance to th ... | 1994 | 7530242 |
| are all carbapenems created equal? | | 1994 | 7530243 |
| airborne dissemination of burkholderia (pseudomonas) cepacia from adult patients with cystic fibrosis. | burkholderia (pseudomonas) cepacia is an increasingly important pathogen in patients with cystic fibrosis but it is unclear how it spreads from patient to patient. a study was undertaken to determine whether b cepacia could be recovered from room air occupied by colonised adult patients with cystic fibrosis. | 1994 | 7530384 |
| [pulmonary melioidosis. report of 4 cases and literature reviewed]. | in this report, we present 4 patients with pulmonary melioidosis. the literatures on epidemiology, clinical manifestation, roentogenology, diagnosis and therapeutics of melioidosis are reviewed. | 1994 | 7530604 |
| cloning, nucleotide sequence, and expression of the plasmid-encoded genes for the two-component 2-halobenzoate 1,2-dioxygenase from pseudomonas cepacia 2cbs. | the two-component nonheme iron dioxygenase system 2-halobenzoate 1,2-dioxygenase from pseudomonas cepacia 2cbs catalyzes the double hydroxylation of 2-halobenzoates with concomitant release of halogenide and carbon dioxide, yielding catechol. the gene cluster encoding this enzyme, cbdabc, was localized on a 70-kbp conjugative plasmid designated pbah1. the nucleotide sequences of cbdabc and flanking regions were determined. in the deduced amino acid sequence of the large subunit of the terminal o ... | 1995 | 7530709 |