| toxicity of mono-, di- and tri-chlorophenols to lux marked terrestrial bacteria, burkholderia species rasc c2 and pseudomonas fluorescens. | burkholderia species rasc and pseudomonas fluorescens were marked with lux genes, encoding for bioluminescence and used to assess the toxicity of mono-, di- and tri-chlorophenols by determining the decline in bioluminescence following exposure to the compounds in aqueous solution. toxicity was expressed as a 50% effective concentration value (ec50, equating to the concentration of compound which caused a 50% decline in bioluminescence. comparing the toxicity values of the compounds showed that, ... | 2001 | 11297395 |
| mother-to-child transmission of burkholderia pseudomallei. | | 2001 | 11302149 |
| [avoidable "fever of unknown origin" due to ralstonia pickettii bacteremia]. | ralstonia pickettii bacteraemia from the atrial part of a ventriculo-atrial shunt was diagnosed as the cause of recurring febrile episodes with systemic toxicity in a 38-year old female. a prior neurosurgical resection of intracerebral cavernoma was complicated by postoperative recurrent meningitis with this non-fermenting gram-negative rod, due to an intra-operative contamination of a in situ shunt, from which only the intracerebral part of the ventriculo-atriostomy had been removed, following ... | 2001 | 11307484 |
| nebulized heparin in burkholderia cepacia colonized adult cystic fibrosis patients. | viscous negatively charged cystic fibrosis (cf) sputum allows colonization by pathogens, inducing a chronic inflammatory response. heparin thins sputum by decreasing the mucin molecule amino group negative charge, altering its intermolecular hydrogen bonding, and ionically shielding its polyionic moieties. it also has an anti-inflammatory effect within the lung. it may, therefore, be useful in the treatment of cf patients. in order to test this, six fully informed burkholderia cepacia colonized ... | 2001 | 11307751 |
| effects of culture conditions on enhancement of 2,4-dinitrotoluene degradation by burkholderia engineered with the vitreoscilla hemoglobin gene. | growth and degradation of 2,4-dinitrotoluene (2,4-dnt) were compared in liquid cultures in shake flasks for burkholderia sp. strain dnt and strain dnt engineered to produce vitreoscilla (bacterial) hemoglobin (strain yv1). parameters varied included aeration rate, initial 2,4-dnt concentration (50 and 200 ppm), and concentration and type of cosubstrate (yeast extract, succinate, casamino acids, and tryptic soy broth). 2,4-dnt degradation increased with increasing cosubstrate concentration and wa ... | 2001 | 11312715 |
| variability and interactions between endophytic bacteria and fungi isolated from leaf tissues of citrus rootstocks. | fungi and bacteria were isolated from surface disinfected leaf tissues of several citrus rootstocks. the principal bacterial species isolated were alcaligenes sp., bacillus spp. (including b. cereus, b. lentus, b. megaterium, b. pumilus, and b. subtilis), burkholderia cepacia, curtobacterium flaccumfaciens, enterobacter cloacae, methylobacterium extorquens, and pantoea agglomerans, with p. agglomerans and b. pumilus being the most frequently isolated species. the most abundant fungal species wer ... | 2001 | 11315114 |
| h(2)o(2) produced by viridans group streptococci may contribute to inhibition of methicillin-resistant staphylococcus aureus colonization of oral cavities in newborns. | in an accompanying report, we showed that viridans group streptococci may prevent methicillin-resistant staphylococcus aureus (mrsa) colonization of the oral cavities of newborns. in the present study, we investigated the mechanism of prevention in vitro. most viridans group streptococci had bacteriocin-like activity and killed mrsa, burkholderia cepacia, enterobacter aerogenes, and pseudomonas aeruginosa; however, escherichia coli, enterobacter cloacae, and candida albicans were resistant. the ... | 2001 | 11317240 |
| cytotoxicity associated with trichloroethylene oxidation in burkholderia cepacia g4. | the effects of trichloroethylene (tce) oxidation on toluene 2-monooxygenase activity, general respiratory activity, and cell culturability were examined in the toluene-oxidizing bacterium burkholderia cepacia g4. nonspecific damage outpaced inactivation of toluene 2-monooxygenase in b. cepacia g4 cells. cells that had degraded approximately 0.5 micromol of tce (mg of cells(-1)) lost 95% of their acetate-dependent o(2) uptake activity (a measure of general respiratory activity), yet toluene-depen ... | 2001 | 11319088 |
| 16s ribosomal dna characterization of nitrogen-fixing bacteria isolated from banana (musa spp.) and pineapple (ananas comosus (l.) merril). | nitrogen-fixing bacteria isolated from banana (musa spp.) and pineapple (ananas comosus (l.) merril) were characterized by amplified 16s ribosomal dna restriction analysis and 16s rrna sequence analysis. herbaspirillum seropedicae, herbaspirillum rubrisubalbicans, burkholderia brasilensis, and burkholderia tropicalis were identified. eight other types were placed in close proximity to these genera and other alpha and beta proteobacteria. | 2001 | 11319127 |
| the burkholderia cepacia fur gene: co-localization with omla and absence of regulation by iron. | the ferric uptake regulator (fur) functions as a transcription repressor of many genes in bacteria in response to iron, but the presence of a functional equivalent of this protein has not been demonstrated in burkholderia cepacia. a segment of the burkholderia pseudomallei fur gene was amplified using degenerate primers and used to identify chromosomal restriction fragments containing the entire fur genes of b. cepacia and b. pseudomallei. these fragments were cloned and sequenced, revealing the ... | 2001 | 11320133 |
| burkholderia cepacia genomovar vi, a new member of the burkholderia cepacia complex isolated from cystic fibrosis patients. | a polyphasic taxonomic study was performed on 23 strains isolated from cystic fibrosis (cf) patients in the usa. these strains were tentatively identified as burkholderia cepacia, burkholderia vietnamiensis and burkholderia or ralstonia sp. using biochemical tests and 16s rdna-based pcr assays. visual comparison of protein profiles indicated that they belonged to a single new group ('group 13'). the polyphasic taxonomic data showed that 18 of these strains represent a new member of the b. cepaci ... | 2001 | 11321071 |
| classification of ralstonia pickettii biovar 3/'thomasii' strains (pickett 1994) and of new isolates related to nosocomial recurrent meningitis as ralstonia mannitolytica sp. nov. | strains isolated independently from two patients could be recognized as ralstonia pickettii biovar 3/'thomasii'. the 16s rdna sequences of these strains and two other strains of r. pickettii biovar 3/'thomasii' clustered at less than 98% similarity versus all other described ralstonia species and at less than 97 % versus the two other r. pickettii biovars. the separate species status of r. pickettii biovar 3/'thomasii' was confirmed by dna-dna hybridization, indicating less than 60% dna homology ... | 2001 | 11321101 |
| complete mineralization of dodecyldimethylamine using a two-membered bacterial culture. | complete degradation of dodecyldimethylamine was achieved using a two-membered bacterial culture isolated from activated sludge. one member, identified as burkholderia cepacia, was capable of degrading the alkyl chain of the molecule. the other member, identified as stenotrophomonas maltophilia, was able to degrade dimethylamine, the product of the former. batch culture experiments revealed that the two-membered culture consisting of b. cepacia and s. maltophilia was based on a commensalistic re ... | 2001 | 11321543 |
| burkholderia cepacia complex: distribution of genomovars among isolates from the maize rhizosphere in italy. | burkholderia cepacia is a 'complex' in which seven genomic species or genomovars have so far been identified. it appears that all seven b. cepacia genomovars are capable of causing infections in vulnerable persons; in particular, the importance of burkholderia multivorans (genomovar ii) and b. cepacia genomovar iii among cystic fibrosis isolates, especially epidemic ones, has been emphasized. in order to acquire a better comprehension of the genomovar composition of environmental populations of ... | 2001 | 11321544 |
| multidrug efflux pumps and antimicrobial resistance in pseudomonas aeruginosa and related organisms. | pseudomonas aeruginosa is an opportunistic human pathogen characterized by an innate resistance to multiple antimicrobial agents. a major contribution to this intrinsic multidrug resistance is provided by a number of broadly-specific multidrug efflux systems, including mexab-oprm and mexxy-oprm. in addition, these and two additional tripartite efflux systems, mexcd-oprj and mexef-oprn, promote acquired multidrug resistance as a result of mutational hyperexpression of the efflux genes. in additio ... | 2001 | 11321581 |
| burkholderia cepacia in cystic fibrosis: novel australian cluster strain without accelerated respiratory deterioration. | to determine whether 4 years of colonization with a novel australian cluster strain (the 'hunter' strain) of burkholderia cepacia (b. cepacia) in cystic fibrosis (cf) patients was associated with more rapid decline in nutritional status and pulmonary function than in non-colonized contemporaries from the same cf clinic. | 2001 | 11328466 |
| detection of heme-binding proteins in epidemic strains of burkholderia cepacia. | a panel of 30 previously characterized strains representing five genomovars from the burkholderia cepacia complex (e. mahenthiralingam, t. coenye, j. w. chung, d. p. speert, j. r. w. govan, p. taylor, and p. vandamme, j. clin. microbiol. 38:910--913, 2000) were examined for their iron protoporphyrin ix-binding ability. these included b. cepacia genomovars i and iii and b. stabilis (formerly b. cepacia genomovar iv), b. multivorans (formerly b. cepacia genomovar ii), and b. vietnamiensis (formerl ... | 2001 | 11329449 |
| gene synthesis, expression in e. coli, and in vitro refolding of pseudomonas sp. kwi 56 and chromobacterium viscosum lipases and their chaperones. | pseudomonas lipases are industrially used as detergent additives, in the food industry, and in organic synthesis. currently, these lipases are either isolated from wild-type strains or overexpressed in recombinant pseudomonas host strains which may be subject to special safety regulations and thus be unsuitable for enzyme engineering via directed evolution. here we describe the heterologous expression of two pseudomonas lipases in escherichia coli. the lipase genes of pseudomonas sp. kwi 56 (rec ... | 2001 | 11330714 |
| diversity in kinetics of trichloroethylene-degrading activities exhibited by phenol-degrading bacteria. | whole-cell kinetics of phenol- and trichloroethylene (tce)-degrading activities expressed by 13 phenol-degrading bacteria were analyzed. the ks (apparent affinity constant in haldane's equation) values for tce were unexpectedly diverse, ranging from 11 microm to over 800 microm. the vmax/ks values for phenol were three orders of magnitude higher than the values for tce in all bacteria analyzed, suggesting that these bacteria preferentially degrade phenol rather than tce. a positive correlation b ... | 2001 | 11330722 |
| burkholderia cepacia: current clinical issues, environmental controversies and ethical dilemmas. | burkholderia cepacia is a plant phytogen and is known as a hardy and versatile organism. over the past two decades it has emerged as a pathogen in the cystic fibrosis (cf) community, with devastating effects. pulmonary colonisation can lead to an accelerated decline in lung function. in some cases, it causes a rapid and progressive pneumonic illness termed "cepacia syndrome", which is untreatable and fatal. b. cepacia is inherently resistant to multiple antibiotics and highly transmissible and v ... | 2001 | 11334134 |
| lipopolysaccharide of burkholderia cepacia and its unique character to stimulate murine macrophages with relative lack of interleukin-1beta-inducing ability. | lipopolysaccharide (lps) of burkholderia cepacia was purified by the conventional phenol-water extraction method (preparation bclps-1), followed by enzymatic treatments with dnase, rnase, trypsin, and proteinase k (preparation bclps-2), and finally by deoxycholate-phenol-water extraction (preparation bclps-3). cells of lps-hyporesponsive c3h/hej mice were activated by both the bclps-1 and the bclps-2 preparations but barely activated by bclps-3. when lps-responsive c3h/hen mice were used as targ ... | 2001 | 11349028 |
| electrokinetic movement and biodegradation of 2,4-dichlorophenoxyacetic acid in silt soil. | the coupling of electrokinetic movement of an organic contaminant, 2,4-dichlorophenoxyacetic acid (2,4-d), through soil and its biodegradation in situ has been demonstrated. in a first experiment, the direction and rate of movement of 2,4-d were determined using homogeneously contaminated soil (864 mg 2,4-d/kg dry weight soil) compacted into six individual compartments, 6 cm long, 3 cm wide, and 4 cm deep. each compartment was bordered by a carbon felt anode and a stainless steel cathode. the ap ... | 2001 | 11353409 |
| separation of 6-deoxy-heptan [correction of 6-deoxy-heptane] from a smooth-type lipopolysaccharide preparation of burkholderia pseudomallei. | smooth-type lipopolysaccharide (lps) of burkholderia pseudomallei has been reported to contain two kinds of o-antigenic polysaccharides, a 1,3-linked homopolymer of 6-deoxy-heptose and a polymer with a repeating unit of -->3)-glucose-(1-->3)-6-deoxy-talose-(1--> with o-acetyl or o-methyl modifications. a lps preparation containing these two polysaccharides was separated by gel-permeation chromatography in this study. chemical analysis of the separated fractions revealed the 6-deoxy-heptan [corre ... | 2001 | 11356562 |
| clinics in diagnostic imaging (57). melioidotic prostatic abscess. | a 46-year-old previously healthy man presented with urosepsis and lower urinary tract obstruction. both urine and blood cultures grew burkholderia pseudomallei. intravenous ceftazidime failed to control the infection. prostatic abscess formation was first detected by transrectal ultrasonography, and the extent was subsequently delineated by computed tomography. the abscess was drained by transurethral resection, which served to eradicate a possible persistent focus of infection. the diagnosis an ... | 2001 | 11361238 |
| 2,4-dichlorophenoxyacetate/alpha-ketoglutarate dioxygenases from burkholderia cepacia 2a and ralstonia eutropha jmp134. | 2,4-dichlorophenoxyacetate (2,4-d)/alpha-ketoglutarate (alpha-kg) dioxygenase has been purified to apparent homogeneity from burkholderia cepacia strain 2a, which utilizes 2,4-d as sole carbon source. the enzyme required ferrous ions, and was a homodimer composed of subunits having an mr of approximately 32,000. the reaction catalysed consumed one mol each of 2,4-d, alpha-kg and dioxygen, with the production of one mol each of succinate, 2,4-dichlorophenol and glyoxylate. maximum activity was ex ... | 2001 | 11368091 |
| the chlorocatechol degradative genes, tfdt-cdef, of burkholderia sp. strain nk8 are involved in chlorobenzoate degradation and induced by chlorobenzoates and chlorocatechols. | the modified-ortho pathway genes responsible for the degradation of chlorocatechols produced from 3- and 4-chlorobenzoate in burkholderia sp. nk8 were cloned and analyzed. the five genes predicted to encode a lysr-type transcriptional regulator, chlorocatechol 1,2-dioxygenase, chloromuconate cycloisomerase, dienelactone hydrolase, and maleylacetate reductase were designated tfdt, tfdc, tfdd, tfde, and tfdf, respectively since they show the highest similarity to the corresponding genes of the chl ... | 2001 | 11368916 |
| cellular aspects of burkholderia cepacia infection. | the gram-negative bacterium burkholderia cepacia has recently emerged as an important opportunistic pathogen in humans. this review focuses on the cellular aspects of b. cepacia infection and the dynamics of the b. cepacia-host cell interaction, including recent advances in our understanding of the ability of b. cepacia to adhere to, enter, and survive intracellularly within human cells. | 2001 | 11369280 |
| bacteriophage p4282, a parasite of ralstonia solanacearum, encodes a bacteriolytic protein important for lytic infection of its host. | to enhance bacterial wilt resistance in tobacco expressing a foreign protein, we isolated the bacteriolytic gene from a bacteriophage that infects ralstonia solanacearum. the bacteriolytic protein of phage p4282 isolated in tochigi prefecture was purified from a lysate of r. solanacearum m4s cells infected with the phage, and its bacteriolytic activity was assayed by following the decrease in the turbidity of suspensions of r. solancacearum m4s cells. the molecular weight of the bacteriolytic pr ... | 2001 | 11370877 |
| dehalogenation, denitration, dehydroxylation, and angular attack on substituted biphenyls and related compounds by a biphenyl dioxygenase. | the attack by the bph-encoded biphenyl dioxygenase of burkholderia sp. strain lb400 on a number of symmetrical ortho-substituted biphenyls or quasi ortho-substituted biphenyl analogues has been investigated. 2,2'-difluoro-, 2,2'-dibromo-, 2,2'-dinitro-, and 2,2'-dihydroxybiphenyl were accepted as substrates. dioxygenation of all of these compounds showed a strong preference for the semisubstituted pair of vicinal ortho and meta carbons, leading to the formation of 2'-substituted 2,3-dihydroxybip ... | 2001 | 11371517 |
| identification of a burkholderia mallei polysaccharide gene cluster by subtractive hybridization and demonstration that the encoded capsule is an essential virulence determinant. | little is known about the virulence factors of burkholderia mallei, the etiologic agent of glanders. we employed subtractive hybridization to identify genetic determinants present in b. mallei but not in burkholderia thailandensis, a non-pathogenic soil microbe. three subtractive hybridization products were mapped to a genetic locus encoding proteins involved in the biosynthesis, export and translocation of a capsular polysaccharide. we identified an insertion sequence (is 407 a) at one end of t ... | 2001 | 11373120 |
| induction of bpha, encoding biphenyl dioxygenase, in two polychlorinated biphenyl-degrading bacteria, psychrotolerant pseudomonas strain cam-1 and mesophilic burkholderia strain lb400. | we investigated induction of biphenyl dioxygenase in the psychrotolerant polychlorinated biphenyl (pcb) degrader pseudomonas strain cam-1 and in the mesophilic pcb degrader burkholderia strain lb400. using a counterselectable gene replacement vector, we inserted a lacz-gm(r) fusion cassette between chromosomal genes encoding the large subunit (bpha) and small subunit (bphe) of biphenyl dioxygenase in cam-1 and lb400, generating cam-10 and lb400-1, respectively. potential inducers of bpha were ad ... | 2001 | 11375179 |
| burkholderia, a genus rich in plant-associated nitrogen fixers with wide environmental and geographic distribution. | the genus burkholderia comprises 19 species, including burkholderia vietnamiensis which is the only known n(2)-fixing species of this bacterial genus. the first isolates of b. vietnamiensis were recovered from the rhizosphere of rice plants grown in a phytotron, but its existence in natural environments and its geographic distribution were not reported. in the present study, most n(2)-fixing isolates recovered from the environment of field-grown maize and coffee plants cultivated in widely separ ... | 2001 | 11375196 |
| controlled comparison of original vented aerobic fan medium with new nonvented bact/alert fa medium for culturing blood. | to evaluate the performance of bact/alert fa (fa) medium, a new aerobic bact/alert fan (fan) medium (organon teknika corporation, durham, n.c.) that does not require the added cost and inconvenience of a venting unit, we inoculated blood specimens from adult patients with suspected sepsis into an original fan aerobic culture bottle and an fa bottle. of 7,745 blood culture sets containing both bottles, 5,256 (68%) met the criteria for adequacy of filling. a total of 466 isolates judged to represe ... | 2001 | 11376041 |
| [quinoline removal in fluidized-bed bioreactor using immobilized cells]. | burkholderia pickettii, capable of using quinoline as sole source of carbon, nitrogen and energy, was immobilized by an innovative pva-h3bo3-gauze method. a fluidized-bed bioreactor, in which immobilized cells were added, was constructed to degrade quinoline in wastewater. when the initial concentrations of quinoline were 100, 350 and 500 mg/l, the bioreactor can remove it completely within 2.5, 6 and 12 hours respectively. a series of zero-order reaction equations were proposed to describe quin ... | 2001 | 11382050 |
| melioidosis: an emerging infection in taiwan? | from january 1982 to may 2000, 17 infections caused by burkholderia pseudomallei were diagnosed in 15 patients in taiwan; almost all the infections were diagnosed from 1994 to may 2000. of the 15 patients, 9 (60%) had underlying diseases, and 10 (67%) had bacteremic pneumonia. thirteen (76%) episodes of infection were considered indigenous. four patients died of melioidosis. seventeen b. pseudomallei isolates, recovered from eight patients from november 1996 to may 2000, were analyzed to determi ... | 2001 | 11384520 |
| root mucilage from pea and its utilization by rhizosphere bacteria as a sole carbon source. | plant roots secrete a complex polysaccharide mucilage that may provide a significant source of carbon for microbes that colonize the rhizosphere. high molecular weight mucilage was separated by high-pressure liquid chromatography gel filtration from low molecular weight components of pea root exudate. purified pea root mucilage generally was similar in sugar and glycosidic linkage composition to mucilage from cowpea, wheat, rice, and maize, but appeared to contain an unusually high amount of mat ... | 2001 | 11386373 |
| burkholderia pseudomallei interferes with inducible nitric oxide synthase (inos) production: a possible mechanism of evading macrophage killing. | burkholderia pseudomallei is a causative agent of melioidosis, a life threatening disease which affects humans and animals in tropical and subtropical areas. this bacterium is known to survive and multiply inside cells such as macrophages. the mechanism of host defense against this bacterium is still unknown. in this study, we demonstrated that b. pseudomallei exhibited unique macrophage activation activity compared with escherichia coli and salmonella typhi. the mouse macrophage cell line (raw ... | 2001 | 11386421 |
| comparison of the polymerase chain reaction and serologic tests for diagnosis of septicemic melioidosis. | for diagnosis of melioidosis, we compared polymerase chain reaction (pcr)-based dna detection and three serologic methods with the culture method currently used as gold standard. the diagnostic values of the serologic methods were evaluated in 130 patients. all these patients resided in an endemic area. an enzyme-linked immunosorbent assay (elisa) gave slightly higher specificity (86.2%) than a dot immunoassay (dot) (85.3%), but was superior to an indirect hemagglutination assay (iha) (79.8%). t ... | 2000 | 11388506 |
| biocatalysis in ionic liquids: markedly enhanced enantioselectivity of lipase. | [reaction: see text] lipase-catalyzed transesterifications in ionic liquids proceeded with markedly enhanced enantioselectivity. it was observed that lipases were up to 25 times more enantioselective in ionic liquids than in conventional organic solvents. | 2001 | 11388853 |
| variable correction of host defense following gene transfer and bone marrow transplantation in murine x-linked chronic granulomatous disease. | chronic granulomatous disease (cgd) is an inherited immunodeficiency in which the absence of the phagocyte superoxide-generating nicotinamide adenine dinucleotide phosphate (nadph) oxidase results in recurrent bacterial and fungal infections. a murine model of x-linked cgd (x-cgd) was used to explore variables influencing reconstitution of host defense following bone marrow transplantation and retroviral-mediated gene transfer. the outcomes of experimental infection with aspergillus fumigatus, s ... | 2001 | 11389011 |
| randomized, double-blind, controlled study of cefoperazone-sulbactam plus cotrimoxazole versus ceftazidime plus cotrimoxazole for the treatment of severe melioidosis. | we conducted a prospective randomized, double-blind, controlled study of cefoperazone-sulbactam (ratio, 1:1; cefoperazone 25 mg/kg/day) plus cotrimoxazole (trimethoprim-sulfamethoxazole [tmp-smz] at a ratio of 80:400; tmp, 8 mg/kg/day) versus ceftazidime (100 mg/kg/day) plus cotrimoxazole (tmp, 8 mg/kg/day) for the treatment of severe melioidosis. of 219 patients enrolled in the study, 102 (47%) had culture-proven melioidosis. these patients were assigned randomly to 2 treatment groups, each wit ... | 2001 | 11389491 |
| structure of an acidic exopolysaccharide produced by the diazotrophic endophytic bacterium burkholderia brasiliensis. | the structure of an exopolysaccharide (eps) produced by burkholderia brasiliensis, a diazotrophic endophytic organism originally isolated from rice roots, has been determined. the bacterium was grown in a synthetic medium, containing mannitol and glutamate, which favours the expression of two anionic epss, which were separated by anion-exchange chromatography. the structure of the repeat unit of eps a, eluted at higher ionic strength, was determined by a combination of methylation analysis, part ... | 2001 | 11389718 |
| chronic granulomatous disease: a case report. | chronic granulomatous disease is one form of the phagocyte function disorder. unlike most patients with chronic granulomatous disease who develop signs and symptoms of chronic and recurrent pyogenic infections during the first 2 years of life, patients with mild forms of the disease may not present until the teenage years or even adulthood. thus, the diagnosis in these mild-form patients is often delayed. this paper describes a patient with the mild form of chronic granulomatous disease. a 7-yea ... | 2001 | 11393130 |
| immunolocalisation of burkholderia cepacia in the lungs of cystic fibrosis patients. | infection by burkholderia cepacia is sometimes fatal in patients with cystic fibrosis (cf), as the organism can cause necrotising pneumonia and septicaemia (the cepacia syndrome), and is resistant to antibiotics. to increase knowledge of the pathogenesis of lung infection, the present study investigated the distribution of b. cepacia in lung explants from nine cf recipients of double lung transplants, of which six were colonised with both b. cepacia and pseudomonas aeruginosa and the other three ... | 2001 | 11393291 |
| [burkholderia cepacia: dangers of a phytopathogen organism for patients with cystic fibrosis]. | burkholderia cepacia is an environmental bacterium, capable of colonising vegetal and animal tissues, involved in human opportunist nosocomial infections, and above all, in pulmonary colonisations in patients with cystic fibrosis. in these patients, infection may be followed by a severe deterioration with bacteraemia, leading to death. moreover, owing to the epidemic spread of some clones within cystic fibrosis communities, strict preventive guidelines have to be instituted. early detection of b ... | 2001 | 11397673 |
| cytokinin production by plant growth promoting rhizobacteria and selected mutants. | one of the proposed mechanisms by which rhizobacteria enhance plant growth is through the production of plant growth regulators. five plant growth promoting rhizobacterial (pgpr) strains produced the cytokinin dihydrozeatin riboside (dhzr) in pure culture. cytokinin production by pseudomonas fluorescens g20-18, a rifampicin-resistant mutant (rif), and two tnphoa-derived mutants (cnt1, cnt2), with reduced capacity to synthesize cytokinins, was further characterized in pure culture using immunoass ... | 2001 | 11400730 |
| distribution of quorum-sensing genes in the burkholderia cepacia complex. | the distribution of quorum-sensing genes among strains from seven genomovars of the burkholderia cepacia complex was examined by pcr. cepr and cepi were amplified from b. cepacia genomovars i and iii, b. stabilis, and b. vietnamiensis. cepr was also amplified from b. multivorans and b. cepacia genomovar vi. bviir were amplified from b. vietnamiensis. all genomovars produced n-octanoyl-l-homoserine lactone and n-hexanoyl-l-homoserine lactone. b. vietnamiensis and b. cepacia genomovar vii produced ... | 2001 | 11402012 |
| synthesis of multiple n-acylhomoserine lactones is wide-spread among the members of the burkholderia cepacia complex. | seventy strains of the burkholderia cepacia complex, which currently comprises six genomic species, were tested for their ability to produce n-acylhomoserine lactone (ahl) signal molecules. using thin layer chromatography in conjunction with a range of ahl biosensors, we show that most strains primarily produce two ahls, namely n-octanoylhomoserine lactone (c8-hsl) and n-hexanoylhomoserine lactone (c6-hsl). furthermore, some strains belonging to b. vietnamiensis (genomovar v) produce additional ... | 2001 | 11403388 |
| acute respiratory failure in melioidosis. | in melioidosis caused by burkholderia pseudomallei, although every organ in the body may be involved, the highest mortality of 73% occurs when the respiratory system is affected. these patients invariably die of acute respiratory failure. most of them also have underlying predisposing factors like diabetes mellitus. | 2001 | 11405563 |
| interventions for treating melioidosis. | melioidosis is an infectious disease caused by a bacterium (burkholderia pseudomallei) found particularly in some areas in the tropics. it is a serious condition which can be fatal. beta lactam antibiotics have dramatically reduced the risk of death, but mortality still remains high. | 2001 | 11405983 |
| in vitro antibiotic susceptibilities of burkholderia mallei (causative agent of glanders) determined by broth microdilution and e-test. | in vitro susceptibilities to 28 antibiotics were determined for 11 strains of burkholderia mallei by the broth microdilution method. the b. mallei strains demonstrated susceptibility to aminoglycosides, macrolides, quinolones, doxycycline, piperacillin, ceftazidime, and imipenem. for comparison and evaluation, 17 antibiotic susceptibilities were also determined by the e-test. e-test values were always lower than the broth dilution values. establishing and comparing antibiotic susceptibilities of ... | 2001 | 11408233 |
| purification and catalytic properties of the chlorophenol 4-monooxygenase from burkholderia cepacia strain ac1100. | burkholderia cepacia strain ac1100 can be induced for the degradation of 2,4,5-trichlorophenol (2,4,5-tcp). we have purified the active enzyme 30-fold to apparent homogeneity with a 44% yield by a two-step chromatographic procedure, and showed that it consists of a single type of subunit of 59 kda based on sds-page using coomassie blue and sypro staining. this enzyme has no bound prosthetic group but requires exogenous addition of fad and nadh to perform the dioxygen-dependent hydroxylation in t ... | 2001 | 11410285 |
| burkholderia fungorum sp. nov. and burkholderia caledonica sp. nov., two new species isolated from the environment, animals and human clinical samples. | a polyphasic taxonomic study that included dna-dna hybridizations, dna base ratio determinations, 16s rdna sequence analyses, whole-cell protein and fatty acid analyses and an extensive biochemical characterization was performed on 16 strains isolated from the environment, animals and human clinical samples. the isolates belonged to the genus burkholderia, were phylogenetically closely related to burkholderia graminis, burkholderia caribensis and burkholderia phenazinium and had g+c contents bet ... | 2001 | 11411678 |
| sugar composition of biofilms produced by paper mill bacteria. | biofilms of paper mill bacteria were cultivated in paper mill white water-simulating conditions on glass slides or stainless steel coupons in a laboratory culture system. the sugar content and composition of the biofilms were analysed and compared with the sugar composition of paper mill slimes. acid methanolysis followed by gas chromatography revealed that burkholderia was the major biofilm producer in pure culture, producing up to 50 microg of biofilm sugar cm(-2) in 5 days in rich medium and ... | 2001 | 11414334 |
| antigenic heterogeneity of lipopolysaccharide among burkholderia pseudomallei clinical isolates. | burkholderia pseudomallei (bp) causes melioidosis, a potentially fatal human infection in the tropics. clinical isolates from different geographical locations have similar morphological and biochemical characteristics. although bp has been reported to possess 2 types of lipopolysaccharide (lps) differing in the chemical structure of their o-polysaccharide (o-ps) component, earlier report demonstrated that the clinical strains exhibited identical lps moieties. recently, we reported antigenic simi ... | 2000 | 11414445 |
| molecular diagnosis of microbial contamination in cosmetic and pharmaceutical products: a review. | molecular methodologies such as adenosine triphosphate (atp) bioluminescence and polymerase chain reaction (pcr)-based assays provide rapid quality control analysis of cosmetic and pharmaceutical finished products and raw materials. using a single enrichment broth for bacteria, yeast, and mold, atp bioluminescence detected microbial contamination within 27 h. samples were automatically lysed to release microbial atp and light production was quantitated using the celsis optocomp. however, to main ... | 2001 | 11417629 |
| the alkane hydroxylase gene of burkholderia cepacia rr10 is under catabolite repression control. | in many microorganisms the first step for alkane degradation is the terminal oxidation of the molecule by an alkane hydroxylase. we report the characterization of a gene coding for an alkane hydroxylase in a burkholderia cepacia strain isolated from an oil-contaminated site. the protein encoded showed similarity to other known or predicted bacterial alkane hydroxylases, although it clustered on a separate branch together with the predicted alkane hydroxylase of a mycobacterium tuberculosis strai ... | 2001 | 11418560 |
| nodulation of legumes by members of the beta-subclass of proteobacteria. | members of the leguminosae form the largest plant family on earth, with around 18,000 species. the success of legumes can largely be attributed to their ability to form a nitrogen-fixing symbiosis with specific bacteria known as rhizobia, manifested by the development of nodules on the plant roots in which the bacteria fix atmospheric nitrogen, a major contributor to the global nitrogen cycle. rhizobia described so far belong exclusively to the alpha-subclass of proteobacteria, where they are di ... | 2001 | 11418858 |
| burkholderia pseudomallei kills the nematode caenorhabditis elegans using an endotoxin-mediated paralysis. | we investigated a non-mammalian host model system for fitness in genetic screening for virulence-attenuating mutations in the potential biowarfare agents burkholderia pseudomallei and burkholderia mallei. we determined that b. pseudomallei is able to cause 'disease-like' symptoms and kill the nematode caenorhabditis elegans. analysis of killing in the surrogate disease model with b. pseudomallei mutants indicated that killing did not require lipopolysaccharide (lps) o-antigen, aminoglycoside/mac ... | 2001 | 11422081 |
| effects of rice seed surface sterilization with hypochlorite on inoculated burkholderia vietnamiensis. | when a combination of hydrogen peroxide and hypochlorite was used to surface sterilize rice seeds, a 10(2)- to 10(4)-fold decrease in cfu was observed during the first 15 h after inoculation of the rice rhizosphere organism burkholderia vietnamiensis tvv75. this artifact could not be eliminated simply by rinsing the seeds, even thoroughly, with sterile distilled water. when growth resumed, a significant increase in the frequency of rifampin- and nalidixic acid-resistant mutants in the population ... | 2001 | 11425720 |
| groel encodes a highly antigenic protein in burkholderia pseudomallei. | no recombinant protein is available for serodiagnosis of melioidosis. in this study, we report the cloning of the groel gene, which encodes an immunogenic protein of burkholderia pseudomallei. bidirectional dna sequencing of groel revealed that the gene contained a single open reading frame encoding 546 amino acid residues with a predicted molecular mass of 57.1 kda. basic local alignment search tool analysis showed that the putative protein encoded by groel is homologous to the chaperonins enco ... | 2001 | 11427437 |
| rapid characterization of the genomovars of the burkholderia cepacia complex by pcr-single-stranded conformational polymorphism (pcr-sscp) analysis. | four polymerase chain reaction (pcr) primer pairs (a-d), specific for the burkholderia cepacia complex of organisms were designed to encompass the entire gene (approximately 1300 bp) from sequence alignments of the rec a operon of b. cepacia. genomic bacterial dna from type strains and wild-type b. cepacia complex isolates of previously determined genomovar status was amplified employing these four primer pairs, as well as a fifth primer set (e) already published. primer sets b, c and e were suc ... | 2001 | 11428880 |
| disproportionate distribution of burkholderia cepacia complex species and transmissibility markers in cystic fibrosis. | several distinct species (genomovars) comprise bacteria previously identified merely as burkholderia cepacia. understanding how these species, collectively referred to as the b. cepacia complex, differ in their epidemiology and pathogenic potential in cystic fibrosis (cf) is important in efforts to refine management strategies. b. cepacia isolates recovered from 606 cf patients receiving care at 132 treatment centers in 105 cities in the united states were assessed to determine species within th ... | 2001 | 11435245 |
| use of selective inhibitors and chromogenic substrates to differentiate bacteria based on toluene oxygenase activity. | in whole-cell studies, two alkynes, 1-pentyne and phenylacetylene, were selective, irreversible inhibitors of monooxygenase enzymes in catabolic pathways that permit growth of bacteria on toluene. 1-pentyne selectively inhibited growth of burkholderia cepacia g4 (toluene 2-monooxygenase [t2mo] pathway) and b. pickettii pko1 (toluene 3-monooxygenase [t3mo] pathway) on toluene, but did not inhibit growth of bacteria expressing other pathways. in further studies with strain g4, chromogenic transfor ... | 2001 | 11438182 |
| clinical outcome after acquisition of burkholderia cepacia in patients with cystic fibrosis. | respiratory disease is the major cause of morbidity and mortality in cystic fibrosis (cf). the significance of burkholderia cepacia (b. cepacia) in the pathogenesis of lung disease in cf is debated, but its exact role remains unclear. | 2001 | 11440408 |
| long-term preservation of strains of burkholderia cepacia, pseudomonas spp. and stenotrophomonas maltophilia isolated from patients with cystic fibrosis. | long-term preservation methods are important in the maintenance of bacteria for downstream research applications. most clinical laboratories have only limited resources for archiving isolates and therefore require cost-effective and simple methods. an effective and cheap storage method using debrinated blood and maintenance at -80 degrees c is described. | 2001 | 11442821 |
| invasion of murine respiratory epithelial cells in vivo by burkholderia cepacia. | pulmonary infections caused by burkholderia cepacia are an important cause of morbidity and mortality in cystic fibrosis (cf) patients. several features suggestive of invasion and intracellular sequestration of b. cepacia in cf are persistence of infection in the face of antibiotic therapy and a propensity to cause bacteraemic infections in patients with cf. a mouse respiratory challenge model was used to investigate the invasion phenotype of b. cepacia in vivo. after intratracheal inoculation, ... | 2001 | 11444769 |
| production of oxalate in the culture supernate of burkholderia pseudomallei. | | 2001 | 11444779 |
| marinobacter strain nce312 has a pseudomonas-like naphthalene dioxygenase. | one strain of bacteria, designated nce312, was isolated from a naphthalene-digesting chemostat culture that was inoculated with creosote-contaminated marine sediment. the strain was isolated based on its ability to grow using naphthalene as a sole carbon source. in addition, the strain degraded 2-methylnaphthalene and 1-methylnaphthalene. analysis of a 16s rrna gene sequence from nce312 placed the isolate in the genus marinobacter. degenerate pcr primers were used to amplify a fragment of a naph ... | 2001 | 11445166 |
| burkholderia pseudomallei virulence: definition, stability and association with clonality. | clinical presentations of melioidosis, caused by burkholderia pseudomallei are protean, but the mechanisms underlying development of the different forms of disease remain poorly understood. in murine melioidosis, the level of virulence of b. pseudomallei is important in disease pathogenesis and progression. in this study, we used b. pseudomallei-susceptible balb/c mice to determine the virulence of a library of clinical and environmental b. pseudomallei isolates from australia and papua new guin ... | 2001 | 11445448 |
| enhanced susceptibility to pulmonary infection with burkholderia cepacia in cftr(-/-) mice. | progressive pulmonary infection is the dominant clinical feature of cystic fibrosis (cf), but the molecular basis for this susceptibility remains incompletely understood. to study this problem, we developed a model of chronic pneumonia by repeated instillation of a clinical isolate of burkholderia cepacia (genomovar iii, et12 strain), an opportunistic gram-negative bacterium, from a case of cf into the lungs of cftr (m1unc-/-) (cftr(-/-)) and congenic cftr(+/+) controls. nine days after the last ... | 2001 | 11447196 |
| comparative in vitro activity of gemifloxacin, ciprofloxacin, levofloxacin and ofloxacin in a north american surveillance study. | the in vitro activity of gemifloxacin, a new fluoroquinolone, was compared to three marketed fluoroquinolones; ciprofloxacin, levofloxacin and ofloxacin against over 4,000 recent clinical isolates covering 29 species isolated in the united states and canada between 1997-1999. based on mic(90)s, gemifloxacin was the most potent fluoroquinolone tested against a majority of gram-positive isolates: streptococcus pneumoniae, penicillin resistant s. pneumoniae, macrolide resistant s. pneumoniae, cipro ... | 2001 | 11448564 |
| [the effect of tannery waste products on viability of selected bacteria from activated sludge]. | in this study we have investigated the influence of sludge on the activity of bacteria strains: acinetobacter baumannii, aeromonas hydrophila and pseudomonas cepacia. to our studies we have taken waste materials used in the chromium compound leather tanning process as well as materials from leather fat and waste materials, in general. it was confirmed that 1:20 dilution of chromium tanning leather waste, retanage and leather fat allowed the survivability of bacteria in all three strains. through ... | 2001 | 11452739 |
| complex of burkholderia cepacia lipase with transition state analogue of 1-phenoxy-2-acetoxybutane: biocatalytic, structural and modelling study. | in a series of four racemic phenoxyalkyl-alkyl carbinols, 1-phenoxy-2-hydroxybutane (1) is enantioselectively acetylated by burkholderia cepacia (formerly pseudomonas cepacia) lipase with an e value > or = 200, whereas for the other three racemates e was found to be < or = 4. to explain the high preference of b. cepacia lipase for (r)-(+)-1, a precursor of its transition state analogue with a tetrahedral p-atom, (r(p),s(p))-o-(2r)-(1-phenoxybut-2-yl)methylphosphonic acid chloride was prepared an ... | 2001 | 11453990 |
| occurrence of burkholderia cepacia in foods and waters: clinical implications for patients with cystic fibrosis. | two hundred forty-eight retail "ready-to-eat" foodstuffs in eight food categories and 134 waters categorized into nine types were analyzed for the presence of the burkholderia cepacia complex of organisms. of these, 14 of 26 (53.8%) samples of raw unpasteurized bovine milk were positive for this organism. consumption of raw unpasteurized milk may therefore act as a potential source of infection with this organism, which is of particular concern for patients with cystic fibrosis, where colonizati ... | 2001 | 11456196 |
| women with cystic fibrosis and their potential for reproduction. | | 2001 | 11462069 |
| diplomats in bangkok and risk of melioidosis. | | 2001 | 11468118 |
| a novel esterase from burkholderia gladioli which shows high deacetylation activity on cephalosporins is related to beta-lactamases and dd-peptidases. | the gene (estb) encoding for a novel esterase (estb) from burkholderia gladioli (formerly pseudomonas marginata) ncppb 1891 was cloned in escherichia coli. sequence analysis showed an open reading frame encoding a polypeptide of 392 amino acid residues, with a molecular mass of about 42 kda. comparison of the amino acid sequence with those of other homologous enzymes indicated homologies to beta-lactamases, penicillin binding proteins and dd-peptidases. the serine residue (ser(75)) which is loca ... | 2001 | 11472796 |
| multiphasic kinetics of transformation of 1,2,4-trichlorobenzene at nano- and micromolar concentrations by burkholderia sp. strain ps14. | the transformation of 1,2,4-trichlorobenzene (1,2,4-tcb) at initial concentrations in nano- and micromolar ranges was studied in batch experiments with burkholderia sp. strain ps14. 1,2,4-tcb was metabolized from nano- and micromolar concentrations to below its detection limit of 0.5 nm. at low initial 1,2,4-tcb concentrations, a first-order relationship between specific transformation rate and substrate concentration was observed with a specific affinity (a(0)(a)) of 0.32 liter. mg (dry weight) ... | 2001 | 11472925 |
| burkholderia cepacia complex infection in italian patients with cystic fibrosis: prevalence, epidemiology, and genomovar status. | the prevalence, epidemiology, and genomovar status of burkholderia cepacia complex strains recovered from italian cystic fibrosis (cf) patients were investigated using genetic typing and species identification methods. four cf treatment centers were examined: two in sicily, one in central italy, and one in northern italy. b. cepacia complex bacteria were isolated from 59 out of 683 cf patients attending these centers (8.6%). for the two geographically related treatment centers in sicily, there w ... | 2001 | 11474009 |
| glanders in a military research microbiologist. | | 2001 | 11474663 |
| ready or not--preparedness for bioterrorism. | | 2001 | 11474670 |
| melioidosis: a clinical model for gram-negative sepsis. | | 2001 | 11478666 |
| flagellin gene pcr-rflp analysis of a panel of strains from the burkholderia cepacia complex. | burkholderia cepacia, an important opportunist pathogen, is genetically heterogeneous. the b. cepacia complex has been subdivided into a number of genospecies or genomovars. a flagellin gene pcr-rflp method was applied to a representative panel of strains of known genomovar. the technique was able to distinguish strains of b. multivorans from other members of the b. cepacia complex on the basis of amplicon size (typical of type i rather than type ii flagellins) with the exception of one genomova ... | 2001 | 11478677 |
| arbitrarily primed pcr and sequencing of 16s rdna for epidemiological typing and species identification of burkholderia cepacia isolates from swedish patients with cystic fibrosis reveal genetic heterogeneity. | to investigate whether arbitrarily primed (ap)-pcr and/or 16s rdna sequencing could be used as rapid methods for epidemiological typing and species identification of clinical burkholderia isolates from patients with cystic fibrosis (cf), a total of 39 clinical b. cepacia isolates, including 33 isolates from 14 cf patients, were fingerprinted. eric-2 primer was used for ap-pcr. the ap-pcr clustering analysis resulted in 14 different clusters at a 70% similarity level. the ap-prc patterns were ind ... | 2001 | 11478687 |
| antibiotic resistance in burkholderia cepacia at two regional cystic fibrosis centres in northern ireland: is there a need for synergy testing? | | 2001 | 11481312 |
| purification and characterization of a lactonase from burkholderia sp. r-711, that hydrolyzes (r)-5-oxo-2-tetrahydrofurancarboxylic acid. | a lactonase hydrolyzing (r)-5-oxo-2-tetrahydrofurancarboxylic acid to d-alpha-hydroxyglutaric acid was purified 170-fold with 2% recovery to near homogeneity from crude extracts of burkholderia sp. r-711, which had been isolated as a bacterium able to assimilate (r)-5-oxo-2-tetrahydrofurancarboxylic acid. the molecular mass was estimated to be 33 kda by gel filtration. the purified preparation migrated as a single band of molecular mass 38 kda upon sds-page. the maximum activity was observed at ... | 2001 | 11491083 |
| limnobacter thiooxidans gen. nov., sp. nov., a novel thiosulfate-oxidizing bacterium isolated from freshwater lake sediment. | two novel thiosulfate-oxidizing strains were isolated from sediment of the littoral zone of a freshwater lake (lake chiemsee, bavaria, germany). the new isolates, designated cs-k1 and cs-k2t, were gram-negative, slightly curved rods with pointed ends that were motile by means of single polar flagella. both strains were obligately aerobic and grew on a variety of organic substrates, but not autotrophically. the utilization of thiosulfate led to an increase in the growth yield, indicating that the ... | 2001 | 11491347 |
| burkholderia ambifaria sp. nov., a novel member of the burkholderia cepacia complex including biocontrol and cystic fibrosis-related isolates. | a polyphasic taxonomic study, including amplified fragment length polymorphism (aflp) fingerprinting, dna-dna hybridizations, dna base-ratio determinations, phylogenetic analysis, whole-cell fatty acid analyses and an extensive biochemical characterization, was performed on 19 burkholderia cepacia-like isolates from the environment and cystic fibrosis (cf) patients. several of the environmental isolates have attracted considerable interest due to their biocontrol properties. the polyphasic taxon ... | 2001 | 11491349 |
| burkholderia cepacia complex: a contraindication to lung transplantation in cystic fibrosis? | previous studies have indicated that pulmonary infection with burkholderia cepacia is associated with poor clinical outcome after lung transplantation in cystic fibrosis (cf). many treatment centers consider b. cepacia infection an absolute contraindication to lung transplantation. however, the b. cepacia complex actually consists of several closely related bacterial species. although each of these has been isolated from cf sputum culture, certain species are much more frequently recovered than ... | 2001 | 11493397 |
| burkholderia pseudomallei-specific recombinant protein and its potential in the diagnosis of melioidosis. | melioidosis is an important public health problem in southeast asia and northern australia. this disease is caused by the gram-negative bacilli, burkholderia pseudomallei. wide spectra of clinical manifestations are observed in melioidosis ranging from asymptomatic to septicemic infection. although serodiagnostic methods of melioidosis have been improved significantly in recent years, a highly specific diagnostic test that can differentiate asymptomatic seropositive individuals and melioidosis p ... | 2001 | 11495298 |
| analysis of transcription of the bph locus of burkholderia sp. strain lb400 and evidence that the orf0 gene product acts as a regulator of the bpha1 promoter. | although gene clusters for the degradation of biphenyls and polychlorobiphenyls have been extensively characterized, comparatively little is known about the regulation of their expression. in the present work, different aspects of transcription of the bph locus of the potent polychlorobiphenyl degrader burkholderia sp. strain lb400 were investigated. an rna blot analysis of the entire gene cluster revealed that the transcription of all genes encoding biphenyl catabolic enzymes responded similarl ... | 2001 | 11495994 |
| structural analysis of the exopolysaccharide from burkholderia caribensis strain mwap71. | burkholderia caribensis strain mwap71 was isolated from rhizosphere soil microaggregates in martinique. the extracellular polysaccharide produced by this strain was found to be composed of d-glucose (d-glc), 6-deoxy-l-talose (l-6dtal), 3-deoxy-d-manno-oct-2-ulosonic acid (kdo), and an o-acetyl group in a molar ratio of 2:1:1:1. the primary structure of the polysaccharide was shown by sugar analysis, electrospray mass spectrometry, partial acid hydrolysis and 1-d and 2-d nmr spectroscopy to consi ... | 2001 | 11502268 |
| differentiation between non-virulent and virulent burkholderia pseudomallei with monoclonal antibodies to the ara+ or ara- biotypes. | burkholderia pseudomallei is the causative agent of melioidosis, a fatal tropical infectious disease endemic in southeast asia. environmental isolates of b. pseudomallei have two distinctive biotypes. some soil isolates are arabinose-assimilators (ara+ biotype) and are non-virulent in experimental animals. the others cannot assimilate arabinose (ara- biotype) and are virulent in experimental animals. the ara- biotype is found in almost all b. pseudomallei clinical isolates. in the present study, ... | 2001 | 11504398 |
| case report: recovery from persistent septicemic melioidosis. | septicemic melioidosis is often fatal despite treatment with antibiotics such as ceftazidime to which burkholderia pseudomallei, the causal pathogen, is sensitive in vitro. we report a near-fatal case of septicemic melioidosis with persistent b. pseudomallei bacteremia despite intravenous ceftazidime in which combination therapy with meropenem and ciprofloxacin, splenectomy and correction of metabolic acidosis allowed for hospital discharge. the choice of antibiotic agents was supported by intra ... | 2001 | 11504412 |
| production in vitro, on different solid culture media, of two distinct exopolysaccharides by a mucoid clinical strain of burkholderia cepacia. | the production of exopolysaccharides (epss) by a mucoid clinical isolate of burkholderia cepacia involved in infections in cystic fibrosis patients, was studied. depending on the growth conditions, this strain was able to produce two different eps, namely ps-i and ps-ii, either alone or together. ps-i is composed of equimolar amounts of glucose and galactose with pyruvate as substituent, and was produced on all media tested. ps-ii is constituted of rhamnose, mannose, galactose, glucose and glucu ... | 2001 | 11506920 |
| biodegradation of quinoline by gel immobilized burkholderia sp. | burkholderia sp, a gram-negative, rod-shaped, aerobe, capable of degrading quinoline was immobilized in calcium alginate gel beads and used for degradation of quinoline in aqueous solution in the reactor. the optimal conditions for immobilization of the microorganism, such as alginate concentration, calcium ion concentration, initial cell loading, hardening time and bead size, were determined with a view to improving the quinoline degradation rate. the characteristics of quinoline degradation by ... | 2001 | 11513389 |
| directed evolution of biphenyl dioxygenase: emergence of enhanced degradation capacity for benzene, toluene, and alkylbenzenes. | biphenyl dioxygenase (bph dox) catalyzes the initial oxygenation of biphenyl and related compounds. bph dox is a multicomponent enzyme in which a large subunit (encoded by the bpha1 gene) is significantly responsible for substrate specificity. by using the process of dna shuffling of bpha1 of pseudomonas pseudoalcaligenes kf707 and burkholderia cepacia lb400, a number of evolved bph dox enzymes were created. among them, an escherichia coli clone expressing chimeric bph dox exhibited extremely en ... | 2001 | 11514531 |
| a case of thoracic empyema due to suppurative melioidosis. | melioidosis is considered a rare disease in hong kong, and its diagnosis and treatment can be difficult. we report the case of a patient who presented with thoracic empyema. the material sampled from the empyema was initially labelled burkholderia cepacia. the diagnosis of melioidosis due to burkholderia pseudomallei could only be made after repeated cultures, and performing arginine dihydrolase and serological tests. the patient was initially treated with imipenem for 2 weeks, and then with cip ... | 2001 | 11514758 |
| viability and stability of biological control agents on cotton and snap bean seeds. | cotton and snap bean were selected for a multi-year, multi-state regional (south-eastern usa) research project to evaluate the efficacy of both commercial and experimental bacterial and fungal biological control agents for the management of damping-off diseases. the goal for this portion of the project was to determine the viability and stability of biological agents after application to seed. the biological seed treatments used included: (1) bacillaceae bacteria, (2) non-bacillaceae bacteria, ( ... | 2001 | 11517723 |