| a rhamnolipid biosurfactant reduces cadmium toxicity during naphthalene biodegradation. | a model cocontaminated system was developed to determine whether a metal-complexing biosurfactant, rhamnolipid, could reduce metal toxicity to allow enhanced organic biodegradation by a burkholderia sp. isolated from soil. rhamnolipid eliminated cadmium toxicity when added at a 10-fold greater concentration than cadmium (890 microm), reduced toxicity when added at an equimolar concentration (89 microm), and had no effect at a 10-fold smaller concentration (8.9 microm). the mechanism by which rha ... | 2000 | 11010924 |
| brain abscess as the presenting feature of melioidosis. | central nervous system involvement in melioidosis is rare and there are only a few reports of the causative organism, burkholderia pseudomallei, causing a brain abscess. we report a patient who presented to us with a brain abscess due to this organism and emphasize the need for a high degree of suspicion for this disease in tropical countries and treatment with the appropriate antibiotics, as the mortality associated with this disease is very high. | 1998 | 11013674 |
| preferential adherence of cable-piliated burkholderia cepacia to respiratory epithelia of cf knockout mice and human cystic fibrosis lung explants. | the burkholderia cepacia complex consists of at least five well-documented bacterial genomovars, each of which has been isolated from the sputum of different patients with cystic fibrosis (cf). although the world-wide prevalence of this opportunist pathogen in cf patients is low (1-3%), 'epidemic' clusters occur in geographically isolated regions. prevalence in some of these clusters is as high as 30-40%. the majority of cf b. cepacia isolates belong to genomovar iii, but the relationship betwee ... | 2000 | 11023184 |
| ralstonia pickettii colonization of patients in an obstetric ward caused by a contaminated irrigation system. | | 2000 | 11023730 |
| molecular cloning and characterization of a chitosanase from the chitosanolytic bacterium burkholderia gladioli strain chb101. | a chitosanase was purified from the culture fluid of the chitino- and chitosanolytic bacterium burkholderia gladioli strain chb101. the purified enzyme (chitosanase a) had a molecular mass of 28 kda, and catalyzed the endo-type cleavage of chitosans having a low degree of acetylation (0-30%). the enzyme hydrolyzed glucosamine oligomers larger than a pentamer, but did not exhibit any activity toward n-acetylglucosamine oligomers and colloidal chitin. the gene coding for chitosanase a (csna) was i ... | 2000 | 11030572 |
| phospholipid compositional changes of five pseudomonad archetypes grown with and without toluene. | bacterial physiological responses to toluene exposure were investigated in five reference pseudomonad strains that express different toluene degradation pathways: pseudomonas putida mt-2, pseudomonas putida f1, burkholderia cepacia g4, burkholderia pickettii pko1, and pseudomonas mendocina kr1. the intact phospholipids of these archetypes, grown with and without toluene, were characterized using liquid chromatography/electrospray ionization/mass spectrometry. all strains showed significant chang ... | 2000 | 11030576 |
| sequencing and characterization of a novel serine metalloprotease from burkholderia pseudomallei. | burkholderia pseudomallei, a gram-negative bacterium is found in the soil and water, mainly in southeast asia and northern australia. it is responsible for melioidosis in human and animals. the bacteria produce several potential virulent factors such as extracellular protease, hemolysin, lipase and lecithinase. the isolation of virulence genes and the study of their functions will contribute to our understanding of bacterial pathogenesis. previous studies have implicated protease as a contributi ... | 2000 | 11040430 |
| rhizosphere competitiveness of trichloroethylene-degrading, poplar-colonizing recombinant bacteria. | indigenous bacteria from poplar tree (populus canadensis var. eugenei 'imperial carolina') and southern california shrub rhizospheres, as well as two tree-colonizing rhizobium strains (atcc 10320 and atcc 35645), were engineered to express constitutively and stably toluene o-monooxygenase (tom) from burkholderia cepacia g4 by integrating the tom locus into the chromosome. the poplar and rhizobium recombinant bacteria degraded trichloroethylene at a rate of 0.8 to 2.1 nmol/min/mg of protein and w ... | 2000 | 11055909 |
| case reports of death during pregnancy in patients with cystic fibrosis--three out of four patients were colonized with burkholderia cepacia. | | 2000 | 11059957 |
| aerobic degradation of mixtures of chlorinated aliphatics by cloned toluene-o-xylene monooxygenase and toluene o-monooxygenase in resting cells. | recombinant strains of escherichia coli constitutively expressing toluene-o-xylene monooxygenase (tomo) of pseudomonas stutzeri ox1 and toluene o-monooxygenase (tom) of burkholderia cepacia g4 were investigated for their ability to oxidize trichloroethylene (tce), 1,1-dichloroethylene (1,1-dce), cis-1,2-dichloroethylene (cis-dce), trans-1,2-dichloroethylene (trans-dce), vinyl chloride (vc), and chloroform (cf), individually as well as in various mixtures. tomo oxidized all of these individual co ... | 2000 | 11064339 |
| the use of protocatechuate dioxygenase for maintaining anaerobic conditions in biochemical experiments. | the study of redox-active systems often requires the maintenance of anaerobic conditions. the glucose oxidase system has often been used to maintain anaerobic conditions, but it has some drawbacks, such as the production of h(2)o(2) and limitations on stability. protocatechuate dioxygenase from burkholderia cepacia and the substrate, protocatechuate, constitute an alternate effective oxygen-scrubbing system that can be used in a wide variety of biochemical experiments. we have shown its suitabil ... | 2000 | 11067739 |
| cloning and analysis of genomic differences unique to burkholderia pseudomallei by comparison with b. thailandensis. | melioidosis is an infectious disease caused by burkholderia pseudomallei. genomic subtractive hybridisation was performed with the closely related avirulent species b. thailandensis to identify virulence genes of b. pseudomallei. the subtractive hybridisation products were highly specific for b. pseudomallei. sequence analysis revealed a number of putative virulence factors, as well as apparently novel sequences of unknown function. the subtracted library contained dna regions of relatively low ... | 2000 | 11073153 |
| genetic and biochemical characterization of 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase and its role in the protocatechuate 4,5-cleavage pathway in sphingomonas paucimobilis syk-6. | protocatechuate (pca) is the key intermediate metabolite in the lignin degradation pathway of sphingomonas paucimobilis syk-6 and is metabolized to pyruvate and oxaloacetate via the pca 4,5-cleavage pathway. we characterized the 4-carboxy-2-hydroxymuconate-6-semialdehyde (chms) dehydrogenase gene (ligc). chms is the 4,5-cleavage product of pca and is converted into 2-pyrone-4,6-dicarboxylate (pdc) by ligc. we found that ligc was located 295 bp downstream of ligb, which encodes the large subunit ... | 2000 | 11073908 |
| bias caused by using different isolation media for assessing the genetic diversity of a natural microbial population. | the influence of isolation medium on the biodiversity of burkholderia cepacia strains recovered from the rhizosphere of zea mays was evaluated by comparing the genetic diversity of isolates obtained by plating serial dilutions of root macerates on the two selective media tb-t and pcat. from each medium, 50 randomly chosen colonies were isolated. on the basis of the restriction patterns of dna coding for 16s rrna (16s rdna) amplified by means of pcr (ardra), all strains isolated from tb-t medium ... | 2000 | 11080375 |
| aquifer protist response and the potential for tce bioremediation with burkholderia cepacia g4 pr1. | bacterivorous protists have been recovered from pristine and contaminated aquifer environments, but the ecological role of these organisms in bioremediation strategies has not been well defined. burkholderia cepacia g4 pr1 constitutively expresses a toluene ortho-monooxygenase (tom) due to a secondary transposition of a tn5 transposable element in a trichloroethylene (tce) degradative plasmid (tom). groundwater and sediment from a potential site for a tce bioremediation field demonstration were ... | 2000 | 11080377 |
| melioidosis research in china. | research on melioidosis and its pathogen has been ongoing in china for more than two decades. it has been demonstrated that the natural foci are located predominantly in hainan, guangdong and guangxi province, where there is a good correlation between soil isolation and the serum prevalence of antibodies to burkholderia pseudomallei. the cases of melioidosis reported up to now are concentrated in the hainan and zhanjiang peninsula. investigations on serotype, virulence, ecology, antibiotic susce ... | 2000 | 11080506 |
| genotype analysis of burkholderia pseudomallei using randomly amplified polymorphic dna (rapd): indicative of genetic differences amongst environmental and clinical isolates. | burkholderia pseudomallei is the causative agent of melioidosis, an infectious disease common in the tropics. melioidosis is most prevalent in the northeastern part of thailand. the diseases has diverse clinical manifestations ranging from mild localized to fatal septicemic forms. the bacterial genetic factors contributing to the severity of melioidosis have not been completely identified. we have developed a genotyping method based upon randomly amplified polymorphic dna (rapd) analysis. eighte ... | 2000 | 11080514 |
| identification of a siderophore receptor required for ferric ornibactin uptake in burkholderia cepacia. | ornibactins are linear hydroxamate siderophores produced by burkholderia cepacia with peptide structures similar to that of pyoverdines produced by the fluorescent pseudomonads. the gene encoding the outer membrane receptor (orba) was identified, sequenced, and demonstrated to have significant homology with hydroxamate receptors produced by other organisms. the orba precursor was predicted to be a protein with a molecular mass of 81 kda. an orba mutant was constructed and demonstrated to be unab ... | 2000 | 11083764 |
| pgstp: an ivet-compatible promoter probe vector conferring resistance to trimethoprim. | | 2000 | 11084853 |
| computational studies of essential dynamics of pseudomonas cepacia lipase. | in order to investigate the interfacial activation of a lipase from pseudomonas cepacia (pcl), molecular dynamics (md) simulations and essential dynamics (ed) analysis were performed in different solvent environments: vacuum and explicit water solvents. starting from the active (open) structure of pcl, the essential dynamics analysis of the simulations revealed large correlated motions, which may be responsible for the activation of the enzyme. fluctuations in the u1 (active-site lid) and u2 dom ... | 2000 | 11089650 |
| outbreak of nosocomial burkholderia cepacia infection and colonization associated with intrinsically contaminated mouthwash. | from august 1996 through june 1998, 69 ventilated, intensive care unit patients at two arizona hospitals had nosocomial respiratory tract cultures positive for burkholderia cepacia. intrinsically contaminated alcohol-free mouthwash was identified by pulsed-field gel electrophoresis as the source of the outbreak. | 2000 | 11089663 |
| expression of a mineral phosphate solubilizing gene from erwinia herbicola in two rhizobacterial strains. | a genetic construction was carried out using the broad host range vector pkt230 and plasmid pmcg898, which encodes the erwinia herbicola pyrroloquinoline quinone (pqq) synthase, a gene involved in mineral phosphate solubilization (mps). the final construction was transformed and expressed in escherichia coli mc1061, and the recombinant plasmids were transferred to burkholderia cepacia is-16 and pseudomonas sp. pss recipient cells by conjugation. clones containing recombinant plasmids produced hi ... | 2001 | 11090687 |
| studies on polyhydroxyalkanoate (pha) accumulation in a pha synthase i-negative mutant of burkholderia cepacia generated by homogenotization. | in the genome of burkholderia cepacia strain ipt64, which accumulates a blend of the two homopolyesters poly(3-hydroxybutyrate), poly(3hb), and poly(3-hydroxy-4-pentenoic acid), poly(3h4pe), from sucrose or gluconate as single carbon source, the polyhydroxyalkanoate (pha) synthase structural gene was disrupted by the insertion of a chloramphenicol-resistant gene cassette (phac1::cm). the suicide vector psup202 harboring phac1::cm was transferred to b. cepacia by conjugation. the inactivated gene ... | 2000 | 11094298 |
| zoospore homing and infection events: effects of the biocontrol bacterium burkholderia cepacia ammdr1 on two oomycete pathogens of pea (pisum sativum l.). | burkholderia cepacia ammdr1 is a biocontrol agent that protects pea and sweet corn seeds from pythium damping-off in field experiments. the goal of this work was to understand the effect of b. cepacia ammdr1 on pythium aphanidermatum and aphanomyces euteiches zoospore homing events and on infection of pea seeds or roots. in vitro, b. cepacia ammdr1 caused zoospore lysis, prevented cyst germination, and inhibited germ tube growth of both oomycetes. b. cepacia ammdr1 also reduced the attractivenes ... | 2000 | 11097889 |
| bacterial lipases from pseudomonas: regulation of gene expression and mechanisms of secretion. | lipases from pseudomonas bacteria are widely used for a variety of biotechnological applications. overexpression in heterologous hosts like escherichia coli failed to produce enzymatically active lipase prompting to study the molecular mechanisms underlying the regulation of lipase gene expression and secretion. the prototype lipase from p. aeruginosa is encoded in a bicistronic operon which is transcribed from two different promotors, one of which depends on the alternative sigma factor rpon (s ... | 2000 | 11099799 |
| unusual respiratory bacterial flora in cystic fibrosis: microbiologic and clinical features. | pulmonary infections continue to be a significant source of morbidity and mortality among patients with cystic fibrosis. although our understanding of the pathogenesis and clinical consequences of pulmonary infections with pseudomonas aeruginosa has increased greatly in recent years, very little is known about potentially emerging pathogens such as burkholderia cepacia complex, stenotrophomonas maltophilia, alcaligenes xylosoxidans, and methicillin-resistant staphylococcus aureus. in this review ... | 2000 | 11100967 |
| kinetic resolution of racemic alpha-methyl-beta-propiothiolactone by lipase-catalyzed hydrolysis. | kinetic resolution of racemic alpha-methyl-beta-propiothiolactone (rac-mptl) using lipases in organic solvent was studied. the lipase from pseudomonas cepacia (pcl) showed the highest (s)-enantioselectivity (e > 100), and cyclohexane containing 1% (v/v) buffer was identified as the best reaction medium for maintaining high enantioselectivity as well as high reaction rate. while the substrate inhibition was not observed up to 300 mm rac-mptl, severe product inhibition was observed even at 50 mm r ... | 2000 | 11101323 |
| identification and detection of stenotrophomonas maltophilia by rrna-directed pcr. | stenotrophomonas maltophilia has recently emerged as an important nosocomial pathogen in immunocompromised patients, in transplant recipients, and in persons with cystic fibrosis (cf). while this organism is nonpathogenic in healthy individuals, it is increasingly associated with morbidity and mortality in susceptible populations. recent studies have indicated that for approximately 10% of cf patients with moderate lung disease, s. maltophilia can be cultured from respiratory tract secretions. i ... | 2000 | 11101555 |
| microbial pathogens associated with cystic fibrosis: special focus on pseudomonas aeruginosa. | cystic fibrosis (cf) is the most common inherited lethal disease among caucasians. the disease is characterized by a high sodium sweat concentration, malabsorption, malnutrition, and chronic bronchopulmonary infection. although cf is a multisystem disorder it is the deterioration of lung function, due mainly to bacterial colonization, that is the major determinant contributing to the high morbidity and mortality of affected patients. a relatively large variety of microbial species can be recover ... | 1998 | 11101911 |
| 16s rdna analysis for characterization of denitrifying bacteria isolated from three agricultural soils. | bacteria capable of denitrification are spread among phylogenetically diverse groups. in the present investigation, molecular methods (amplified ribosomal dna restriction analysis (ardra) and partial 16s rdna gene sequencing) were used to determine the genetic diversity of culturable denitrifying soil bacteria. the purpose of this work was to study the microbial density and diversity of denitrifying communities isolated from two luvisols and a rendosol. the denitrifying bacterial density was sig ... | 2000 | 11102689 |
| [evaluation of bacterial contamination in disinfectants for domestic use]. | to evaluate disinfectants for domestic use for the presence of bacteria, identify them, and determine their tolerance level to benzalkonium chloride. | 2000 | 11105107 |
| mouse model of sublethal and lethal intraperitoneal glanders (burkholderia mallei). | sixty male balb/c mice were inoculated intraperitoneally with either a sublethal or a lethal dose of burkholderia mallei china 7 strain, then killed at multiple time points postinoculation. histopathologic changes were qualitatively similar in both groups and consisted of pyogranulomatous inflammation. in sublethal study mice, changes were first seen at 6 hours in mediastinal lymph nodes, then in spleen, liver, peripheral lymph nodes, and bone marrow at day 3. these changes generally reached max ... | 2000 | 11105952 |
| characterization of burkholderia pseudomallei isolated in thailand and malaysia. | a total of 35 burkholderia pseudomallei isolates from thailand (16 clinical and eight soil isolates) and malaysia (seven animal, two isolate each from clinical and soil) were investigated by their antimicrobial resistance, plasmid profiles and were typed by randomly amplified polymorphic dna analysis. all isolates were found to be resistant to six or more of the 12 antimicrobial agents tested. only two small plasmids of 1.8 and 2.4 megadalton were detected in two clinical isolates from thailand. ... | 2000 | 11109011 |
| travel-associated burkholderia pseudomallei infection (melioidosis) in a patient with cystic fibrosis: a case report. | in september 1997, a 25-year-old italian woman with cystic fibrosis (cf) spent 3 weeks in thailand. in august 1998, her pulmonary function rapidly declined, with productive cough and intermittent fever. chest x-ray films revealed diffuse, small, patchy opacities in the upper lobes. burkholderia pseudomallei (bp) was isolated from specimens of the patient's sputum and was identified by means of 16s rdna sequencing. the diagnosis of melioidosis was serologically confirmed. continuous therapy with ... | 2001 | 11112669 |
| phylogeny of the major head and tail genes of the wide-ranging t4-type bacteriophages. | we examined a number of bacteriophages with t4-type morphology that propagate in different genera of enterobacteria, aeromonas, burkholderia, and vibrio. most of these phages had a prolate icosahedral head, a contractile tail, and a genome size that was similar to that of t4. a few of them had more elongated heads and larger genomes. all these phages are phylogenetically related, since they each had sequences homologous to the capsid gene (gene 23), tail sheath gene (gene 18), and tail tube gene ... | 2001 | 11114936 |
| detection of bacterial virulence genes by subtractive hybridization: identification of capsular polysaccharide of burkholderia pseudomallei as a major virulence determinant. | burkholderia pseudomallei, the etiologic agent of melioidosis, is responsible for a broad spectrum of illnesses in humans and animals particularly in southeast asia and northern australia, where it is endemic. burkholderia thailandensis is a nonpathogenic environmental organism closely related to b. pseudomallei. subtractive hybridization was carried out between these two species to identify genes encoding virulence determinants in b. pseudomallei. screening of the subtraction library revealed a ... | 2001 | 11119486 |
| kinetic studies of the production of nitric oxide (no) and tumour necrosis factor-alpha (tnf-alpha) in macrophages stimulated with burkholderia pseudomallei endotoxin. | the mechanism by which burkholderia pseudomallei survives in macrophages is not clearly understood. in this study, we demonstrated that the mouse macrophage cell line (raw 264.7) treated with lipopolysaccharide (lps) from b. pseudomallei (bp-lps) produced significantly less no and tnf-alpha compared with those stimulated with the lps from escherichia coli and salmonella typhi. the time required for the bp-lps to trigger substantial no and tnf-alpha release was at least 30 min, compared with < 5 ... | 2000 | 11122236 |
| burkholderia pseudomallei--abscess in an unusual site. | meloidosis in a unusual site has been reported in a child. complete identification of the organism has been presented. | 1999 | 11127385 |
| synthesis of neoglycoproteins containing d-glycero-d-talo-oct-2-ulopyranosylonic acid (ko) ligands corresponding to core units from burkholderia and acinetobacter lipopolysaccharide. | glycal esters of kdo derivatives were converted into 2,3-anhydro intermediates, which were transformed into d-glycero-d-talo-oct-2-ulopyranosylonic acid (ko), as well as 3-o- and 4-o-p-nitrobenzoyl-ko derivatives. the exo-allyl orthoester derivative, methyl [5,7,8-tri-o-acetyl-4-o-(4-nitrobenzoyl)-2,3-o-[(1-exo-allyloxy)-ethylidene]-d-glycero-beta-d-talo-oct-2-ulopyranos]onate, prepared from the 4-o-pnbz-protected ko derivative, was elaborated into the alpha-ko allyl ketoside, the reducing disac ... | 2000 | 11128584 |
| the treatment of respiratory pseudomonas infection in cystic fibrosis: what drug and which way? | pseudomonas aeruginosa is a non-capsulate and non-sporing gram-negative bacillus that most commonly affects the lower respiratory system in humans. burkholderia (previously pseudomonas) cepacia has emerged as an important respiratory pathogen in patients with cystic fibrosis (cf). the ability of p. aeruginosa to persist and multiply in moist environments and equipment, such as humidifiers in hospital wards, bathrooms, sinks and kitchens, maybe of importance in cross-infection. p. aeruginosa infe ... | 2000 | 11129122 |
| monoclonal antibody-based rapid identification of burkholderia pseudomallei in blood culture fluid from patients with community-acquired septicaemia. | a monoclonal antibody-based latex agglutination (mab-la) test was employed for the rapid identification of burkholderia pseudomallei in blood culture fluid from patients with community-acquired septicaemia. these patients were admitted to 12 hospitals in the northeastern part of thailand which is a region known to be endemic for melioidosis. blood samples were collected and immediately added to the blood culture bottles which were incubated in either automated (five hospitals) or manual (seven h ... | 2000 | 11129718 |
| regioselective enzymatic aminoacylation of lobucavir to give an intermediate for lobucavir prodrug. | synthesis of lobucavir prodrug, l-valine, [(1s,2r,3r)-3-(2-amino-1,6-dihydro-6-oxo-9h-purin-9-yl)-2-(hydroxymethyl) cyclobutyl]methyl ester monohydrochloride (bms 233866), requires regioselective coupling of one of the two hydroxyl groups of lobucavir (bms 180194) with valine. either hydroxyl group of lobucavir could be selectively aminoacylated with valine by using enzymatic reactions. n-[(phenylmethoxy)carbonyl]-l-valine, [(1r,2r,4s)-2-(2-amino-6-oxo-1h-purin-9-yl)-4-(hydroxymethyl)cyclobutyl] ... | 2000 | 11131158 |
| infections in e-beta thalassemia. | infection is a major complication and the leading cause of death in thalassemia, especially e-beta thalassemia. the spectrum of infections in e-beta thalassemia include mild and severe infections, therapy-related infections such as yersinia enterocolitica infection associated with desferrioxamine (dfo) therapy, and transfusion-transmitted disease, as well as unique infections such as with pythiosis. prospective studies in thailand indicate that patients with e-beta thalassemia had more frequent ... | 2000 | 11132234 |
| contemporary assessment of antimicrobial susceptibility testing methods for polymyxin b and colistin: review of available interpretative criteria and quality control guidelines. | the emergence of infections caused by multidrug-resistant pseudomonas aeruginosa and acinetobacter spp. has necessitated the search for alternative parenteral agents such as the polymyxins. the national committee for clinical laboratory standards (nccls) documents do not currently provide interpretative criteria for the testing of the polymyxins, colistin and polymyxin b. therefore, an evaluation of the antimicrobial activity of colistin and polymyxin b was initiated using 200 bloodstream infect ... | 2001 | 11136768 |
| clinical aspects of chronic granulomatous disease. | data from a registry of 368 patients with chronic granulomatous disease (cgd) documenta shift in the most common infecting organisms away from staphylococci and enteric bacteria to aspergillus species, although staphylococci remain a threat. a. nidulans appears to have a particular virulence in cgd. burkholderia cepacia sepsis/pneumonia was the second most lethal infection in patients in the registry. seventy-six percent of registry patients had the x-linked recessive (xlr) form of cgd. choriore ... | 2001 | 11138621 |
| characterization of bphf, a rieske-type ferredoxin with a low reduction potential. | bphf is a small, soluble, rieske-type ferredoxin involved in the microbial degradation of biphenyl. the rapid, anaerobic purification of a heterologously expressed, his-tagged bphf yielded 15 mg of highly homogeneous recombinant protein, rcbphf, per liter of cell culture. the reduction potential of rcbphf, determined using a highly oriented pyrolytic graphite (hopg) electrode, was -157+/- 2 mv vs the standard hydrogen electrode (she) (20 mm mops, 80 mm kcl, and 1 mm dithiothreitol, ph 7.0, 22 de ... | 2001 | 11141059 |
| bystander activation of cd8+ t cells contributes to the rapid production of ifn-gamma in response to bacterial pathogens. | the bacterium burkholderia pseudomallei causes a life-threatening disease called melioidosis. in vivo experiments in mice have identified that a rapid ifn-gamma response is essential for host survival. to identify the cellular sources of ifn-gamma, spleen cells from uninfected mice were stimulated with b. pseudomallei in vitro and assayed by elisa and flow cytometry. costaining for intracellular ifn-gamma vs cell surface markers demonstrated that nk cells and, more surprisingly, cd8(+) t cells w ... | 2001 | 11145690 |
| [bact-alert system for hemocultures: evaluation of terminal subcultures]. | few laboratory microbiological procedures are as important as the isolation of microorganisms from blood. to evaluate the usefulness of the terminal subcultures, 5669 blood cultures giving negative results after 7 days of incubation in the bact/alert system (organon teknika) were studied. bottles were distributed as follows: 1562 adult aerobic bottles, 119 adult anaerobic bottles, 3960 pediatric bottles and 28 fan bottles. from 5669 blood cultures, 10 subcultures that yielded growth had not been ... | 2000 | 11149151 |
| cloning and characterization of estc from burkholderia gladioli, a novel-type esterase related to plant enzymes. | by screening a genomic library of burkholderia gladioli (formerly pseudomonas marginata) for clones exhibiting esterolytic activity, the gene for a novel-type esterase (estc) showing significant homology to plant enzymes could be isolated. high homology was found to two hydroxynitrile lyases originating from hevea brasiliensis (tropical rubber tree) and manihot esculenta (cassava), and to two proteins from oryza sativa (rice) that are specifically induced upon infection by pseudomonas syringae p ... | 2000 | 11152069 |
| recent developments in pulmonary infections. | | 2000 | 11153574 |
| pseudomonas antimicrobica attafuah and bradbury 1990 is a junior synonym of burkholderia gladioli (severini 1913) yabuuchi et al. 1993. | comparison of the 16s rdna sequence of pseudomonas antimicrobica lmg 18920t with published 16s rdna sequences from other pseudomonads indicated that pseudomonas antimicrobica belongs to the genus burkholderia, with burkholderia gladioli, burkholderia glumae and burkholderia plantarii as its closest neighbours. dna-dna hybridizations confirmed that pseudomonas antimicrobica and burkholderia gladioli represent the same species. strain lmg 18920t and other burkholderia gladioli strains were also in ... | 2000 | 11155989 |
| nitrogen fixation genes in an endosymbiotic burkholderia strain. | in this paper we report the identification and characterization of a dna region containing putative nif genes and belonging to a burkholderia endosymbiont of the arbuscular mycorrhizal fungus gigaspora margarita. a genomic library of total dna extracted from the fungal spores was also representative of the bacterial genome and was used to investigate the prokaryotic genome. screening of the library with azospirillum brasilense nifhdk genes as the prokaryotic probes led to the identification of a ... | 2001 | 11157237 |
| burkholderia cepacia genomovar iii is a common plant-associated bacterium. | a polyphasic taxonomic study involving dna-dna hybridization, whole-cell protein electrophoresis, and 16s ribosomal dna sequence analysis revealed that a group of burkholderia cepacia-like organisms isolated from the rhizosphere or tissues of maize, wheat, and lupine belong to b. cepacia genomovar iii, a genomic species associated with "cepacia syndrome" in cystic fibrosis patients. the present study also revealed considerable protein electrophoretic heterogeneity within this species and demonst ... | 2001 | 11157274 |
| comparative specificities of two evolutionarily divergent hydrolases involved in microbial degradation of polychlorinated biphenyls. | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate (hopda) hydrolase (bphd) is a key determinant in the aerobic transformation of polychlorinated biphenyls (pcbs) by burkholderia sp. strain lb400 (s. y. k. seah, g. labbé, s. nerdinger, m. johnson, v. snieckus, and l. d. eltis, j. biol. chem. 275:15701-15708, 2000). to determine whether this is also true in divergent biphenyl degraders, the homologous hydrolase of rhodococcus globerulus p6, bphd(p6), was hyperexpressed, purified to apparent homogeneity, a ... | 2001 | 11160080 |
| in vitro resistance of burkholderia cepacia complex isolates to reactive oxygen species in relation to catalase and superoxide dismutase production. | the burkholderia cepacia complex comprises groups of genomovars (genotypically distinct strains with very similar phenotypes) that have emerged as important opportunistic pathogens in cystic fibrosis (cf) patients. the inflammatory response against bacteria in the airways of cf individuals is dominated by polymorphonuclear cells and involves the generation of oxidative stress, which leads to further inflammation and tissue damage. bacterial catalase, catalase-peroxidase and superoxide dismutase ... | 2001 | 11160804 |
| identification of the acid phosphatase (acpa) gene homologues in pathogenic and non-pathogenic burkholderia spp. facilitates tnphoa mutagenesis. | burkholderia pseudomallei and burkholderia mallei are pathogens responsible for disease in both humans and animals. burkholderia thailandensis, while phylogenetically similar, is considered avirulent in comparison. these three species exhibit phosphatase activity when grown on media containing chromogenic substrates such as 5-bromo-4-chloro-3-indolyl phosphate (xp). tn5-ot182 mutagenesis has been utilized to isolate mutants of b. pseudomallei and b. thailandensis unable to hydrolyse xp. sequence ... | 2001 | 11160805 |
| the chlorobenzoate dioxygenase genes of burkholderia sp. strain nk8 involved in the catabolism of chlorobenzoates. | burkholderia sp. nk8 grows abundantly on 3-chlorobenzoate (3cb),4-chlorobenzoate (4cb) and benzoate. the genes encoding the oxidation of (chloro)benzoates (cbeabcd) and catechol (cata, catbc), the lysr-type regulatory gene cber and the gene cbee with unknown function, all of which form a single cluster in nk8, were cloned and analysed. the protein sequence of chlorobenzoate 1,2-dioxygenase (cbeabc) is 50-65% identical to the benzoate dioxygenase (benabc) of acinetobacter sp. adp1, toluate dioxyg ... | 2001 | 11160806 |
| antibiotic susceptibility of burkholderia pseudomallei from tropical northern australia and implications for therapy of melioidosis. | from a prospective melioidosis study commencing in 1989 at royal darwin hospital, 170 initial isolates of burkholderia pseudomallei were available for susceptibility testing. of these 163 (96%) were susceptible to meropenem/imipenem, ceftazidime, trimethoprim-sulphamethoxazole (smx/tmp) and doxycycline. seven (4%) showed primary resistance; three had low-level resistance to smx/tmp, one to ceftriaxone and amoxycillin/clavulanate (amox/ca) and three to doxycycline. of 167 patients who survived th ... | 2001 | 11165114 |
| new broad-host-range promoter probe vectors based on the plasmid rk2 replicon. | broad-host-range plasmid rk2-based promoter probe vectors with a known nucleotide sequence were constructed. in the absence of an upstream promoter, the expression of two tested reporter genes (luc and lacz) in escherichia coli was virtually zero, while insertion of the ptrc promoter resulted in strong inducer-dependent expression. the lacz-based vectors were mobilized into pseudomonas fluorescens st, pseudomonas putida kt2442, sphingomonas spp. and burkholderia spp. lb400, and expression analys ... | 2001 | 11167001 |
| study on the pathophysiology of experimental burkholderia pseudomallei infection in mice. | burkholderia pseudomallei is the etiological agent of melioidosis, a potentially fatal disease occurring in man and animals. the aim of this study was to investigate the pathophysiological course of experimental melioidosis, and to identify the target organs, in an animal model. for this purpose swiss mice were infected intraperitoneally with the virulent strain b. pseudomallei 6068. the bacterial load of various organs was quantified daily by bacteriological analysis and by an enzyme-linked imm ... | 2001 | 11172992 |
| detection of immunoglobulins m and g using culture filtrate antigen of burkholderia pseudomallei. | igm and igg based elisa systems were developed using the culture filtrate antigen (cfa) of burkholderia pseudomallei. the assays were evaluated using 95 sera from 66 septicemic cases and 47 sera from 20 cases with localized melioidosis. in addition 65 sera from culture negative cases that were also serologically negative for other endemic infections clinically suspected of melioidosis were included. these were compared with sera from 260 non-melioidosis cases, 169 sera from individuals with high ... | 2001 | 11173184 |
| phenotypic and phylogenetic characterization of burkholderia (pseudomonas) sp. strain lb400. | the relevant phenotypic traits and phylogenetic relationships between burkholderia (pseudomonas) sp. strain lb400 and b. cepacia atcc 25416(t) were compared to determine the degree to which these two strains might be related. strain lb400 degrades chlorinated biphenyls and has been a model system for potential use in the bioremediation of polychlorinated biphenyls, while some strains of b. cepacia are plant and human pathogens. the fatty acid methyl ester profile, sole carbon source utilization, ... | 2001 | 11178728 |
| screening and characterization of trehalose-oleate hydrolyzing lipase. | various soil samples were collected to screen the presence of microorganisms which have ability to degrade toe. one strain (aku-883) with good toe degrading activity was isolated and identified as burkholderia cepacia and the extracellular enzyme was purified to homogeneity. the purification was achieved by ultrafiltration, super q anion-exchange chromatography and superdex 200hr gel-filtration in the presence of triton x. the enzyme was purified to 85-fold, and specific activity of 4.910 ku mg ... | 2001 | 11179657 |
| expression of 2-halobenzoate dioxygenase genes (cbdsabc) involved in the degradation of benzoate and 2-halobenzoate in burkholderia sp. th2. | burkholderia sp. th2, isolated from soil, utilizes 2-chlorobenzoate (2cb) and benzoate (ba) as its sole source of carbon and energy. the genes for 2-halobenzoate dioxygenase (cbdabc) from burkholderia sp. th2 were cloned and sequenced. the predicted amino acid sequences of all the gene products are highly similar to the cbd gene products of pseudomonas sp. 2cbs. disruption of the promoter region of cbda resulted in loss of growth on 2cb and ba, indicating that these genes are involved in the gro ... | 2001 | 11179677 |
| activity of plant flavonoids against antibiotic-resistant bacteria. | thirty eight plant-derived flavonoids representing seven different structural groups were tested for activities against antibiotic-resistant bacteria using the disc-diffusion assay and broth dilution assay. among the flavonoids examined, four flavonols (myricetin, datiscetin, kaempferol and quercetin) and two -flavones (flavone and luteolin) exhibited inhibitory activity against methicillin-resistant staphylococcus aureus (mrsa). myricetin was also found to inhibit the growth of multidrug-resist ... | 2001 | 11180521 |
| melioidosis and pandora's box in the lao people's democratic republic. | melioidosis has not been recognized previously in laos, but within months of starting a prospective study of community acquired septicemia in vientiane, 2 patients with melioidosis were identified. one was a previously healthy, 44-year-old female rice farmer who presented with supraclavicular lymphadenitis and the other was a 74-year-old man with diabetes and renal calculi who was receiving corticosteroids and had septicemia and septic arthritis. | 2001 | 11181133 |
| a cluster exposed: structure of the rieske ferredoxin from biphenyl dioxygenase and the redox properties of rieske fe-s proteins. | ring-hydroxylating dioxygenases are multicomponent systems that initiate biodegradation of aromatic compounds. many dioxygenase systems include rieske-type ferredoxins with amino acid sequences and redox properties remarkably different from the rieske proteins of proton-translocating respiratory and photosynthetic complexes. in the latter, the [fe2s2] clusters lie near the protein surface, operate at potentials above +300 mv at ph 7, and express ph- and ionic strength-dependent redox behavior. t ... | 2000 | 11188691 |
| distribution of immunoglobulin classes and igg subclasses against a culture filtrate antigen of burkholderia pseudomallei in melioidosis patients. | the class and subclass distribution of antibody response to the culture filtrate antigen (cfa) of burkholderia pseudomallei was examined in the sera of 45 septicaemic and 17 localised melioidosis cases and 40 cases clinically suspected of melioidosis and the results were compared with those from high-risk and healthy control groups. the geometric mean titre index (gmti) values for all classes and subclasses of immunoglobulins examined were higher for sera from the proven and clinically suspected ... | 2001 | 11192506 |
| the isolation of burkholderia cepacia in a hospital setting in sri lanka. | burkholderia cepacia is widely prevalent in nature. the natural habitat of this organism is soil, water and vegetation, but the hospital environment remains the main source of infection. it causes a wide variety of infections in hospitalised patients. since there are no reports of its prevalence in sri lanka, a data retrieval and analysis was undertaken to determine its incidence among patients at sri jayawardenepura general hospital (sjgh). | 2000 | 11192990 |
| identification of the metabolically active members of a bacterial community in a polychlorinated biphenyl-polluted moorland soil. | the presumptive metabolically active members of a bacterial community in a moorland soil in germany, highly polluted with polychlorinated biphenyls (pcbs), were identified by sequencing of cloned reverse transcription-polymerase chain reaction (rt-pcr) amplification products of 16s rrna generated from total rna extracts. analysis of the 16s rrna clone library revealed a considerable diversity of metabolically active bacteria in the soil, despite the acidic ph and high concentrations of pcbs. clo ... | 1999 | 11207739 |
| rrna probe-based cell fishing of bacteria. | we have developed a new, cultivation-independent, fast and flexible method for the rrna-targeted probe-based enrichment of bacteria. the target cells were labelled by in situ hybridization with biotinylated polyribonucleotide probes. these probes were generated by in vitro transcription of amplified rdna of a variable region in domain iii of the 23s rrna molecules. the probes were about 300 nucleotides in length and were labelled by incorporation of biotin-utp during the transcription. probes we ... | 1999 | 11207744 |
| development and characterization of a lux-modified 2,4-dichlorophenol-degrading burkholderia sp. rasc. | lux-marked biosensors for assessing the toxicity and bioremediation potential of polluted environments may complement traditional chemical techniques. luxcdabe genes were introduced into the chromosome of the 2,4-dichlorophenol (2,4-dcp)-mineralizing bacterium, burkholderia sp. rasc c2, by biparental mating using the tn4431 system. experiments revealed that light output was constitutive and related to cell biomass concentration during exponential growth. the transposon insertion was stable and d ... | 1999 | 11207758 |
| infection with burkholderia cepacia in cystic fibrosis: outcome following lung transplantation. | as a result of concern over excessive mortality after lung transplantation, many transplant programs refuse to accept cystic fibrosis (cf) patients infected with burkholderia cepacia. as a significant proportion of patients with cf in our community are infected with this organism, we have continued to provide lung transplantation as an option. a retrospective review was conducted of medical records of all patients with cf transplanted between march 1988 and september 1996. fifty-six transplant p ... | 2001 | 11208624 |
| [pathogenicity of burkholderia pseudomallei: extracellular and surface antigen functions]. | the data of literature and the results of investigations carried out by the authors on the analysis of b. pseudomallei pathogenicity factors. they include mucoid, endotoxin, lecithinase, proteases, hemolysins, etc. in addition to fimbriae and pili, the adhesive properties of b. pseudomallei are supposed to be associated with surface capsule-like structures whose composition includes ag8. the characterization of exoproteases, hemolysins and lethal toxins is given. the virulence and immunogenicity ... | 2000 | 11210655 |
| type iii secretion systems and pathogenicity islands. | some bacterial pathogens have evolved by acquiring pathogenicity islands (pis), which are clusters of genes encoding virulence traits. pis encoding the secretion of effector molecules via type iii secretion (tts) systems have been discovered in several gram-negative pathogens. tts systems are involved in contact-dependent secretion of virulence factors and can facilitate delivery of toxins directly into target cells. the expanding list of bacteria found to contain clusters of tts genes includes ... | 2001 | 11211218 |
| genotypic and chemotaxonomic evidence for the reclassification of pseudomonas woodsii (smith 1911) stevens 1925 as burkholderia andropogonis (smith 1911) gillis et al. 1995. | it was reported before that [pseudomonas] woodsii and burkholderia andropogonis are phenotypically indistinguishable and probably represent the same taxon, for which the name b. andropogonis has been proposed. in the present study, it was found that [p.] woodsii and b. andropogonis strains were indistinguishable by whole-cell protein electrophoresis and have a highly similar cellular fatty acid composition. a high dna-dna binding value of 95% was found between the type strains of both species. i ... | 2001 | 11211258 |
| transient asceptic arthritis of knees in a patient with melioidosis. | osteomyelitis and septic arthritis are known manifestations of melioidosis. whether reactive arthritis can occur as a sequele to b. pseudomallei infection, is yet to be seen. as more and more cases of melioidosis will be reported in future, this aspect has to be kept in mind. | 2000 | 11214516 |
| preferential occurrence of diazotrophic endophytes, azoarcus spp., in wild rice species and land races of oryza sativa in comparison with modern races. | several diazotrophic species of azoarcus spp. occur as endophytes in the pioneer plant kallar grass. the purpose of this study was to screen asian wild rice and cultivated oryza sativa varieties for natural association with these endophytes. populations of culturable diazotrophs in surface-sterilized roots were characterized by 16s rdna sequence analysis, and azoarcus species were identified by genomic fingerprints. a. indigens and azoarcus sp. group c were detected only rarely, whereas azoarcus ... | 2000 | 11220300 |
| outbreak of subclinical mastitis in a flock of dairy sheep associated with burkholderia cepacia complex infection. | an outbreak of subclinical mastitis in a flock of 620 milking sheep was investigated. microbiological and epidemiological analyses identified the causative agent as belonging to the burkholderia cepacia complex (formerly pseudomonas cepacia). every ewe in the milking flock was individually tested for subclinical mastitis on two separate occasions, 6 weeks apart, by the california (rapid) mastitis test (cmt). the proportion of cmt-positive ewes was 69 of 393 (17.6%) on the first sampling and 27 o ... | 2001 | 11230416 |
| phenotypic methods for determining genomovar status of the burkholderia cepacia complex. | recent taxonomic advances have demonstrated that burkholderia cepacia is a cluster of at least seven closely related genomic species (or genomovars) collectively referred to as the b. cepacia complex, all of which may cause infections among cystic fibrosis patients and other vulnerable individuals. thus, it is important for clinical microbiologists to be able to differentiate genomovars. prior to this study, 361 b. cepacia complex isolates and 51 isolates easily confused with b. cepacia complex ... | 2001 | 11230429 |
| [immunotropic and immunogenic properties of burkholderia pseudomallei surface and membrane antigens]. | the immunotropic and immunogenic properties of some chromatographic fractions of b. pseudomallei surface antigenic complex, as well as the preparations of b. pseudomallei outer and cytoplasmic membranes, were studied. the difference between the biopolymers under study in cytotoxicity, humoral and cell-mediated immunity characteristics, phagocytic activity were established. some antigenic fractions (b, c, c1, h) showed perceptible protective activity (25-60%) in experiments on mice infected with ... | 2001 | 11236497 |
| melioidosis splenic abscess--an unusual presentation as osteomyelitis of rib. | we report an unusual case of splenic melioidosis abscess presenting as osteomyelitis. | 2001 | 11242625 |
| different portions of the maize root system host burkholderia cepacia populations with different degrees of genetic polymorphism. | in order to acquire a better understanding of the spatial and temporal variations of genetic diversity of burkholderia cepacia populations in the rhizosphere of zea mays, 161 strains were isolated from three portions of the maize root system at different soil depths and at three distinct plant growth stages. the genetic diversity among b. cepacia isolates was analysed by means of the random amplified polymorphic dna (rapd) technique. a number of diversity indices (richness, shannon diversity, ev ... | 2000 | 11243257 |
| role of commensal relationships on the spatial structure of a surface-attached microbial consortium. | a flow cell-grown model consortium consisting of two organisms, burkholderia sp. lb400 and pseudomonas sp. b13(fr1), was studied. these bacteria have the potential to interact metabolically because pseudomonas sp. b13(fr1) can metabolize chlorobenzoate produced by burkholderia sp. lb400 when grown on chlorobiphenyl. the expected metabolic interactions in the consortium were demonstrated by high performance liquid chromatography (hplc) analysis. the spatial structure of the consortium was studied ... | 2000 | 11243263 |
| burkholderia gladioli: recurrent abscesses in a patient with cystic fibrosis. | we describe a case of an adult patient with cystic fibrosis who developed chronic pulmonary infection and multiple episodes of soft tissue abscesses with burkholderia gladioli; this organism should be added to the list of potential pathogens for individuals with cystic fibrosis. | 2001 | 11243758 |
| regulation of ornibactin biosynthesis and n-acyl-l-homoserine lactone production by cepr in burkholderia cepacia. | the cepr-cepi quorum-sensing system has been shown to regulate production of the siderophore ornibactin, extracellular proteases, and n-octanoyl-homoserine-l-lactone (ohl) in burkholderia cepacia strain k56-2. to examine the effect of cepir on production of other siderophores, cepr mutants were constructed in strains that produce pyochelin in addition to salicylic acid and ornibactins. pc715j-r1 (cepr::tp) hyperproduced ornibactin but produced parental levels of pyochelin and salicylic acid, sug ... | 2001 | 11244059 |
| fulminant septic melioidosis after a vacation in thailand. | severe infection with burkholderia pseudomallei (formerly pseudomonas pseudomallei), the bacterium causing melioidosis, is a common cause of acquired septicaemia in south-east asia and northern australia. a few cases of infected travellers returning to european countries have been reported. melioidosis is a tropical disease, the clinical presentation ranging from asymptomatic infection to fulminant sepsis. predisposing conditions such as impaired cellular immunity, preexisting renal failure or d ... | 2000 | 11244616 |
| bacterial community changes and enrichment of burkholderia-like bacteria induced by chlorinated benzoates in a peat-forest soil-microcosm. | bacterial community shifts in a peat-forest soil spiked with 3-chlorobenzoate (3cba) or 2,5-dichlorobenzoate (2,5dcb) were monitored by pcr-amplification of the v6 to v8 regions of the 16s rrna and rdna, followed by separation of the amplicons by temperature gradient gel electrophoresis. 3cba disappeared to non-detectable levels after 15 days by a biologically mediated process, while 2,5dcb remained at the initial concentration values. the experiments were conducted under microcosms systems. add ... | 2000 | 11249031 |
| the gender gap in cystic fibrosis survival. | females with cystic fibrosis have significantly higher mortality than males from age 1 to age 20, resulting in an approximate four-year difference in median survival age. survival is associated with a lack of colonization by pathogenic bacteria, as well as better pulmonary function, weight for height, and fitness. this statistically significant gender gap has been observed for decades and, therefore, is not likely to be the result of differential response to one of the newer treatments for cysti ... | 1999 | 11252861 |
| incidence of the cbla major subunit pilin gene amongst burkholderia species. | pcr-amplification has been used to screen 75 isolates of the burkholderia cepacia complex for the cbla pilin gene. pcr-amplified products of the correct size (664 bp) were cloned and sequenced and the sequences compared. apart from in the control, epidemic cystic fibrosis (cf)-associated b. cepacia lineage we also identified, for the first time, cbla genes in a unique, non-cf clinical isolate from france and a plant (onion) pathogenic isolate from italy. the sequence of the cbla gene amplified f ... | 2001 | 11257549 |
| [pulmonary function after liver transplantation in cystic fibrosis. report of four cases]. | the aim of this study was to evaluate pulmonary function in four patients with cystic fibrosis (cf) after liver transplantation. | 2001 | 11262249 |
| susceptibility of burkholderia cepacia and other pathogens of importance in cystic fibrosis to u.v. light. | to investigate the potential usefulness of u.v. germicidal irradiation (uvgi) in preventing the spread of burkholderia cepacia, an important pathogen in cystic fibrosis (cf), the in-vitro susceptibility of b. cepacia to uvgi was determined. five strains were exposed to uvgi from a 7.2-w source. burkholderia cepacia was less susceptible to uvgi than other important cf-related pathogens, namely staphylococcus aureus and pseudomonas aeruginosa, but was more susceptible than stenotrophomonas maltoph ... | 2001 | 11264740 |
| antimicrobial activity of streblus asper leaf extract. | bactericidal activity was found in the 50% ethanol (v/v) extract of streblus asper leaves. the extract possessed a selective bactericidal activity towards streptococcus, especially to streptococcus mutans which has been shown to be strongly associated with dental caries. the extract had no effect on cultures of escherichia coli, staphylococcus aureus and pseudomonas aeruginosa, staphylococcus coagulase positive, staphylococcus coagulase negative, serratia marcescens, klebsiella pneumoniae, enter ... | 2001 | 11268109 |
| invasion of human type ii pneumocytes by burkholderia cepacia. | burkholderia cepacia is known to invade and survive within respiratory epithelial cells. previous studies have employed transformed cell lines and it is not known whether the bacterium is capable of manifesting the same phenomena in primary cell culture. two strains of b. cepacia of environmental (nctc 10661) and clinical origin (c1359) were examined for their ability to invade and survive (over a 24 h period) within type ii pneumocytes in primary culture using a gentamicin-ceftazidime antibioti ... | 2001 | 11273742 |
| multicentre study of the in vitro activity of cefepime, a broad-spectrum cephalosporin, compared to other broad-spectrum agents. | the in vitro activity of cefepime was compared to that of a range of other broad-spectrum agents, using a gradient diffusion mic method, against 995 recent clinical isolates of enterobacteriaceae, pseudomonas aeruginosa, other nonfermentative gram-negative bacilli, staphylococci (except oxacillin-resistant staphylococcus aureus), streptococci, enterococci, and aerobic gram-positive bacilli. cefepime had excellent activity against enterobacteriaceae, including eight presumptive extended-spectrum ... | 2001 | 11280610 |
| combined use of 16s ribosomal dna and 16s rrna to study the bacterial community of polychlorinated biphenyl-polluted soil. | the bacterial diversity assessed from clone libraries prepared from rrna (two libraries) and ribosomal dna (rdna) (one library) from polychlorinated biphenyl (pcb)-polluted soil has been analyzed. a good correspondence of the community composition found in the two types of library was observed. nearly 29% of the cloned sequences in the rdna library were identical to sequences in the rrna libraries. more than 60% of the total cloned sequence types analyzed were grouped in phylogenetic groups (a c ... | 2001 | 11282645 |
| chronic melioidosis in a patient with cystic fibrosis. | burkholderia pseudomallei, the causative agent of melioidosis, is endemic in southeast asia and northern australia, where it can be found in soil and surface water. we report a case of chronic pulmonary melioidosis in a patient with cystic fibrosis who had traveled to an area where b. pseudomallei is endemic. | 2001 | 11283117 |
| capsule essential for fatal tropical disease pathogen. | | 2001 | 11286767 |
| cloning and characterisation of male in burkholderia pseudomallei. | no recombinant protein is available for serodiagnosis or skin test in the diagnosis of melioidosis. this report describes the cloning of the male gene, which encodes an immunogenic protein of burkholderia pseudomallei. bi-directional dna sequencing of male revealed that the gene contained a single open reading frame encoding 416 amino acid residues with a predicted molecular mass of 44.4 kda. blast analysis showed that the putative protein encoded by male is homologous to the maltose-binding pro ... | 2001 | 11289518 |
| stochastic-convective transport with nonlinear reaction and mixing: application to intermediate-scale experiments in aerobic biodegradation in saturated porous media. | aerobic biodegradation of benzoate by pseudomonas cepacia sp. in a saturated heterogeneous porous medium was simulated using the stochastic-convective reaction (scr) approach. a laboratory flow cell was randomly packed with low permeability silt-size inclusions in a high permeability sand matrix. in the scr upscaling approach, the characteristics of the flow field are determined by the breakthrough of a conservative tracer. spatial information on the actual location of the heterogeneities is not ... | 2001 | 11291477 |