Publications

glyburide is anti-inflammatory and associated with reduced mortality in melioidosis.	patients with diabetes mellitus are more prone to bacterial sepsis, but there are conflicting data on whether outcomes are worse in diabetics after presentation with sepsis. glyburide is an oral hypoglycemic agent used to treat diabetes mellitus. this k(atp)-channel blocker and broad-spectrum atp-binding cassette (abc) transporter inhibitor has broad-ranging effects on the immune system, including inhibition of inflammasome assembly and would be predicted to influence the host response to infect ...	2011	21293047
communication with a growing family: diffusible signal factor (dsf) signaling in bacteria.	many pathogenic bacteria use cell-cell signaling to regulate the expression of factors contributing to virulence. bacteria produce signals of diverse structural classes. the signal molecule known as diffusible signal factor (dsf) is a cis-unsaturated fatty acid that was first described in the plant pathogen xanthomonas campestris. recent work has shown that structurally related molecules produced by the unrelated bacteria burkholderia cenocepacia and pseudomonas aeruginosa regulate virulence, bi ...	2011	21227698
a product analog bound form of 3-oxoadipate-enol-lactonase (pcad) reveals a multifunctional role for the divergent cap domain.	lactones are a class of structurally diverse molecules that serve essential roles in biological processes ranging from quorum sensing to the aerobic catabolism of aromatic compounds. not surprisingly, enzymes involved in the bioprocessing of lactones are often targeted for protein engineering studies with the potential, for example, of optimized bioremediation of aromatic pollutants. the enol-lactone hydrolase (elh) represents one such class of targeted enzymes and catalyzes the conversion of 3- ...	2011	21237173
macrophage and galleria mellonella infection models reflect the virulence of naturally occurring isolates of b. pseudomallei, b. thailandensis and b. oklahomensis.	burkholderia pseudomallei is the causative agent of melioidosis, a tropical disease of humans with a variable and often fatal outcome. in murine models of infection, different strains exhibit varying degrees of virulence. in contrast, two related species, b. thailandensis and b. oklahomensis, are highly attenuated in mice. our aim was to determine whether virulence in mice is reflected in macrophage or wax moth larvae (galleria mellonella) infection models.	2011	21241461
burkavidin: a novel secreted biotin-binding protein from the human pathogen burkholderia pseudomallei.	the avidin-biotin technology has many applications, including molecular detection; immobilization; protein purification; construction of supramolecular assemblies and artificial metalloenzymes. here we present the recombinant expression of novel biotin-binding proteins from bacteria and the purification and characterization of a secreted burkavidin from the human pathogen burkholderia pseudomallei. expression of the native burkavidin in escherichia coli led to periplasmic secretion and formation ...	2011	21241807
the oxalic acid biosynthetic activity of burkholderia mallei is encoded by a single locus.	although it is known that oxalic acid provides a selective advantage to the secreting microbe our understanding of how this acid is biosynthesized remains incomplete. this study reports the identification, cloning, and partial characterization of the oxalic acid biosynthetic enzyme from the animal bacterial pathogen, burkholderia mallei. the discovered gene was named oxalate biosynthetic component (obc)1. complementation of burkholderia oxalate defective (bod)1, a burkholderia glumae mutant that ...	2011	21242070
photothermal nanoblade for large cargo delivery into mammalian cells.	it is difficult to achieve controlled cutting of elastic, mechanically fragile, and rapidly resealing mammalian cell membranes. here, we report a photothermal nanoblade that utilizes a metallic nanostructure to harvest short laser pulse energy and convert it into a highly localized explosive vapor bubble, which rapidly punctures a lightly contacting cell membrane via high-speed fluidic flows and induced transient shear stress. the cavitation bubble pattern is controlled by the metallic structure ...	2011	21247066
human immune responses to burkholderia pseudomallei characterized by protein microarray analysis.	we aimed to determine the antibody and t cell responses to burkholderia pseudomallei of humans to select candidate vaccine antigens.	2011	21300673
acceptance criteria for identification results of gram-negative rods by mass spectrometry.		2011	21233293
versatile metabolic adaptations of ralstonia eutropha h16 to a loss of pdhl, the e3 component of the pyruvate dehydrogenase complex.	a previous study reported that the tn5-induced poly(3-hydroxybutyric acid) (phb)-leaky mutant ralstonia eutropha h1482 showed a reduced phb synthesis rate and significantly lower dihydrolipoamide dehydrogenase (dhldh) activity than the wild-type r. eutropha h16 but similar growth behavior. insertion of tn5 was localized in the pdhl gene encoding the dhldh (e3 component) of the pyruvate dehydrogenase complex (pdhc). taking advantage of the available genome sequence of r. eutropha h16, observation ...	2011	21296938
classification and identification of the burkholderia cepacia complex: past, present and future.	the burkholderia cepacia complex is a group of closely related species with conflicting biological properties. triggered by the devastating effect of pulmonary infections in cystic fibrosis patients, the scientific community generated an unusually large amount of taxonomic data for these bacteria during the past 15 years. this review presents the polyphasic, multilocus and genomic methodology used for the classification and identification of these bacteria. the current state-of-the-art demonstra ...	2011	21257278
demethylation of methylarsonic acid by a microbial community.	arsenic is one of the most widespread environmental carcinogens and has created devastating human health problems worldwide, yet little is known about mechanisms of biotransformation in contaminated regions. methylarsonic acid [mas(v)], extensively utilized as an herbicide, is largely demethylated to more toxic inorganic arsenite, which causes environmental problems. to understand the process of demethylation of methylarsenicals, soil samples commonly used on florida golf courses were studied. s ...	2011	21272184
bpib05, a novel metagenome-derived hydrolase acting on n-acylhomoserine lactones.	the n-acyl-homoserine lactones (n-ahls) play an important role in bacterial cell-cell signaling. up to date, however, only a few different experimentally proven classes of n-ahl ring-cleaving enzymes are known. here we report on the isolation and biochemical characterization of a novel hydrolase derived from the soil metagenome and acting on n-ahls. the identified protein designated bpib05 is weakly similar to hypothetical proteins from bacteroides fragilis, the draft genomes of two burkholderia ...	2011	21215778
autophagy targeting of listeria monocytogenes and the bacterial countermeasure.	autophagy acts as an intrinsic defense system against intracellular bacterial survival. recently, multiple cellular pathways that target intracellular bacterial pathogens to autophagy have been described. these include the atg5/lc3 pathway, which targets shigella, the ubiquitin (ub)-ndp52-lc3 pathway, which targets group a streptococcus (gas) and salmonella typhimurium, the ub-p62-lc3 pathway, which targets mycobacterium tuberculosis, listeria monocytogenes and s. typhimurium, and the diacylglyc ...	2011	21193840
differential effects of frozen storage on the molecular detection of bacterial taxa that inhabit the nasopharynx.		2011	21261963
hospital-wide outbreak of burkholderia contaminans caused by prefabricated moist washcloths.	we experienced a hospital outbreak of burkholderia contaminans (burkholderia cepacia group k) in a german university hospital with two campuses. cases were defined as the microbiological detection of b. cepacia complex (bcc) in any clinical specimen sent to the laboratory during 30 june to 21 october 2008. species identification of bcc was performed by reca gene sequencing, followed by pulsed-field gel electrophoresis (pfge; spei digest) for clonal identity. in total, 61 bcc-positive cases were ...	2011	21216034
use of culture and molecular analysis to determine the effect of antibiotic treatment on microbial community diversity and abundance during exacerbation in patients with cystic fibrosis.	anaerobic bacteria are increasingly regarded as important in cystic fibrosis (cf) pulmonary infection. the aim of this study was to determine the effect of antibiotic treatment on aerobic and anaerobic microbial community diversity and abundance during exacerbations in patients with cf.	2011	21270069
monitoring biodegradative enzymes with nanobodies raised in camelus dromedarius with mixtures of catabolic proteins.	functional studies of biodegradative activities in environmental microorganisms require molecular tools for monitoring catabolic enzymes in the members of the native microbiota. to this end, we have generated repertories of single-domain v(hh) fragments of camel immunoglobulins (nanobodies) able to interact with multiple proteins that are descriptors of environmentally relevant processes. for this, we immunized camelus dromedarius with a cocktail of up to 12 purified enzymes that are representat ...	2011	21219561
marr homologs with urate-binding signature.	bacteria that associate with living hosts require intricate mechanisms to detect and respond to host defenses. part of the early host defense against invading bacteria is the production of reactive oxygen species, and xanthine oxidase is one of the main producers of such agents. the end-product of the enzymatic activity of xanthine oxidase, urate, was previously shown to be the natural ligand for deinococcus radiodurans-encoded hucr and it was shown to attenuate dna binding by agrobacterium tume ...	2011	21207455
conformational plasticity of the calcium-binding pocket in the burkholderia glumae lipase: remodeling induced by mutation of calcium coordinating residues.	most bacterial lipases bind one or more ca²+ atoms at different locations and are a suitable case of study for investigating structural effects related to calcium binding, depletion, or mutation of calcium-binding sites. generally ca²+ in microbial lipases can play a crucial role in the stabilization of the whole three-dimensional structure by mediating long-range effects. it has been recently demonstrated that calcium binding influences thermal stability of burkholderia glumae lipase (bgl) thro ...	2011	20857506
il-8 released from human lung epithelial cells induced by cystic fibrosis pathogens burkholderia cepacia complex affects the growth and intracellular survival of bacteria.	burkholderia cepacia complex (bcc) is a group of gram-negative pulmonary pathogens associated with life-threatening infections in patients with cystic fibrosis (cf). the airway epithelium plays a crucial role in the initiation and modulation of inflammatory responses to these pathogens. interleukin (il)-8 released from epithelial cells is a potent chemoattractant for neutrophils. the aims of this study were to compare the il-8 response to bcc infection in different epithelial cell types and to i ...	2011	20829108
bactericidal effects of non-thermal argon plasma in vitro, in biofilms and in the animal model of infected wounds.	non-thermal (low-temperature) physical plasma is under intensive study as an alternative approach to control superficial wound and skin infections when the effectiveness of chemical agents is weak due to natural pathogen or biofilm resistance. the purpose of this study was to test the individual susceptibility of pathogenic bacteria to non-thermal argon plasma and to measure the effectiveness of plasma treatments against bacteria in biofilms and on wound surfaces. overall, gram-negative bacteria ...	2011	20829396
a novel light-dependent selection marker system in plants.	photosensitizers are common in nature and play diverse roles as defense compounds and pathogenicity determinants and as important molecules in many biological processes. toxoflavin, a photosensitizer produced by burkholderia glumae, has been implicated as an essential virulence factor causing bacterial rice grain rot. toxoflavin produces superoxide and h₂o₂ during redox cycles under oxygen and light, and these reactive oxygen species cause phytotoxic effects. to utilize toxoflavin as a selection ...	2011	20731786
ecological succession in long-term experimentally evolved biofilms produces synergistic communities.	many biofilm populations are known for their exceptional biodiversity, but the relative contributions of the forces that could produce this diversity are poorly understood. this uncertainty grows in the old, well-established communities found on many natural surfaces and in long-term, chronic infections. if the prevailing interactions among species within biofilms are positive, productivity should increase with diversity, but if they tend towards competition or antagonism, productivity should de ...	2011	20811470
burkholderia cepacia complex: emerging multihost pathogens equipped with a wide range of virulence factors and determinants.	the burkholderia cepacia complex (bcc) comprises at least 17 closely-related species of the beta-proteobacteria subdivision, widely distributed in natural and man-made inhabitats. bcc bacteria are endowed with an extraordinary metabolic diversity and emerged in the 1980s as life-threatening and difficult-to-treat pathogens among patients suffering from cystic fibrosis. more recently, these bacteria became recognized as a threat to hospitalized patients suffering from other diseases, in particula ...	2011	20811541
enhancing functional expression of heterologous burkholderia lipase in escherichia coli.	functional expression of lipase from burkholderia sp. c20 (lip) in various cellular compartments of escherichia coli was explored. the poor expression in the cytoplasm of e. coli was improved by several strategies, including coexpression of the cytoplasmic chaperone groel/es, using a mutant e. coli host strain with an oxidative cytoplasm, and protein fusion technology. fusing lip with the n-terminal peptide tags of t7pk, dsba, and dsbc was effective in enhancing the solubility and biological act ...	2011	20730511
the plant pathogen pseudomonas fuscovaginae contains two conserved quorum sensing systems involved in virulence and negatively regulated by rsal and the novel regulator rsam.	pseudomonas fuscovaginae is a gram-negative fluorescent pseudomonad pathogenic towards several plant species. despite its importance as a plant pathogen, no molecular studies of virulence have thus far been reported. in this study we show that p. fuscovaginae possesses two conserved n-acyl homoserine lactone (ahl) quorum sensing (qs) systems which we designated pfsi/r and pfvi/r. the pfsi/r system is homologous to the bvii/r system of burkholderia vietnamiensis and produces and responds to c10-h ...	2011	20701623
the effect of dornase alfa on ventilation inhomogeneity in patients with cystic fibrosis.	outcome measures to assess therapeutic interventions in cystic fibrosis (cf) patients with mild lung disease are lacking. our aim was to determine if the lung clearance index (lci) can detect a treatment response to dornase alfa in paediatric cf patients with normal spirometry. cf patients between 6-18 yrs of age with fev(1 )≥ 80% pred were eligible. in a crossover design, 17 patients received 4 weeks of dornase alfa and placebo in a randomised sequence separated by a 4-week washout period. the ...	2011	20693248
prhklm genes of ralstonia solanacearum encode novel activators of hrp regulon and are required for pathogenesis in tomato.	the genes in the hrp regulon encode the proteins composing type iii secretion system in ralstonia solanacearum. the hrp regulon is positively controlled by hrpb, and hrpb expression is activated by both hrpg and prhg. we have identified three genes, prhk, prhl, and prhm, which positively control the hrp regulon in strain oe1-1. these genes are likely to form an operon, and this operon is well conserved in the genera ralstonia and burkholderia. this indicates that the operon is not specific to th ...	2011	21241356
a conference report from "down under": talking autophagy at ozbio2010.	ozbio2010 was held at the melbourne convention and exhibition centre, september 26 to october 1, 2010. this international conference catered to researchers in several fields having complementary interests including biochemistry, molecular biology, cell biology, plant physiology and health-related research. it was held under the auspices of two major international scientific societies, iubmb and faobmb, and the combio2010 collective (representing nine professional societies and groupings from aus ...	2011	21116128
perasafe, virkon and bleach are bactericidal for burkholderia pseudomallei, a select agent and the cause of melioidosis.		2011	20832143
increased electrical output when a bacterial abts oxidizer is used in a microbial fuel cell.	microbial fuel cells (mfcs) are a technology that provides electrical energy from the microbial oxidation of organic compounds. most mfcs use oxygen as the oxidant in the cathode chamber. this study examined the formation in culture of an unidentified bacterial oxidant and investigated the performance of this oxidant in a two-chambered mfc with a proton exchange membrane and an uncoated carbon cathode. dna, fame profile and characterization studies identified the microorganism that produced the ...	2011	20853166
detection of autoinducer (ai-2)-like activity in food samples.	the contamination, survival, and possible foodborne disease outbreaks are major issues confronting the food industry. however, from a microbial perspective, any food whether natural or processed is just another environmental niche that is available for colonization. quorum sensing or cell-cell communication is a process by which microorganisms are thought to communicate with each other using a variety of small molecules termed autoinducers. the autoinducer ai-2 is thought to be a universal signa ...	2011	21031305
mucoid and nonmucoid burkholderia cepacia complex bacteria in cystic fibrosis infections.	infection with burkholderia cepacia complex (bcc) bacteria in cystic fibrosis (cf) is associated with an unpredictable rate of pulmonary decline. some bcc, but not others, elaborate copious mucoid exopolysaccharide, endowing them with a gross mucoid phenotype, the clinical significance of which has not been described.	2011	20709823
genetic characterization of root-nodule bacteria associated with acacia salicina and a. stenophylla (mimosaceae) across south-eastern australia.	symbiotic relationships between legumes and nitrogen-fixing soil micro-organisms are of ecological importance in plant communities worldwide. for example, nutrient-poor australian soils are often dominated by shrubby legumes (e.g. species of acacia). however, relatively few studies have quantified patterns of diversity, host-specificity and effectiveness of these ecologically important plant-microbe interactions. in this study, 16s rrna gene sequence and pcr-rflp analyses were used to examine ba ...	2011	20228207
burkholderia oxyphila sp. nov., a bacterium isolated from acidic forest soil that catabolizes (+)-catechin and its putative aromatic derivatives.	a novel bacterium, designated strain ox-01(t), was isolated from acidic soil, taxonomically investigated and identified as an agent that catabolizes (+)-catechin into taxifolin. strain ox-01(t) is a gram-reaction-negative, aerobic, non-sporulating, non-motile and rod-shaped bacterium. 16s rrna gene sequence analysis identified this strain as a member of the genus burkholderia and occupying a phylogenetic position closest to, but clearly distinct from, burkholderia sacchari. strain ox-01(t) does ...	2011	20207808
phylogenetic relationships and diversity of β-rhizobia associated with mimosa species grown in sishuangbanna, china.	in order to investigate the genetic diversity of rhizobia associated with various exotic and invasive species in tropical mainland china, 116 bacterial isolates were obtained from mimosa root nodules collected from sishuangbanna and yuanjiang districts of yunnan province. isolated rhizobia were characterized by rflp analysis of 16s rrna genes, sds-page of whole-cell proteins and box-pcr. most of the isolated strains were identified as β-rhizobia belonging to diverse populations of burkholderia a ...	2011	20228206
endofungal bacterium controls its host by an hrp type iii secretion system.	burkholderia rhizoxinica and rhizopus microsporus form a unique symbiosis in which intracellular bacteria produce the virulence factor of the phytopathogenic fungus. notably, the host strictly requires endobacteria to sporulate. in this study, we show that the endofungal bacteria possess a type iii secretion system (t3ss), which has a crucial role in the maintenance of the alliance. mutants defective in type iii secretion show reduced intracellular survival and fail to elicit sporulation of the ...	2011	20720578
fe-superoxide dismutase and 2-hydroxy-1,4-benzoquinone reductase preclude the auto-oxidation step in 4-aminophenol metabolism by burkholderia sp. strain ak-5.	burkholderia sp. strain ak-5 converts 4-aminophenol to maleylacetic acid via 1,2,4-trihydroxybenzene, which is unstable in vitro and non-enzymatically auto-oxidized to 2-hydroxy-1,4-benzoquinone. crude extract of strain ak-5 retarded the auto-oxidation and reduced the substrate analogue, 2,6-dimethoxy-1,4-benzoquinone, in the presence of nadh. the two enzymes responsible were purified to homogeneity. the deduced amino acid sequence of the enzyme that inhibited the auto-oxidation showed a high le ...	2011	20480210
photodynamic antimicrobial chemotherapy (pact) in combination with antibiotics for treatment of burkholderia cepacia complex infection.	this study aimed to determine if photodynamic antimicrobial chemotherapy (pact) was effective in the treatment of burkholderia cepacia complex infection and whether a synergistic effect was evident if pact was used in combination with antibiotics. the susceptibility of both planktonic and biofilm cultures of b. cepacia complex strains to methylene blue (mb) and meso-tetra(n-methyl-4-pyridyl)porphine tetra-tosylate (tmp)-mediated pact was determined alone and in combination with antibiotics used ...	2012	22079165
Isolation, characterization and docking studies of 2,3-dihydroxybiphenyl 1,2-dioxygenase from an activated sludge metagenome.	A 2,3-dihydroxybiphenyl-1,2-dioxygenase gene (designated as bphC_meta) was identified in activated sludge metagenome by PCR. This gene shared 99% sequence identity with BphC from Burkholderia xenovorans LB400. The enzyme was purified from recombinant Escherichia coli with a subunit molecular mass of 32 ± 1 kDa. It was optimally active at pH 9.0 and 40°C, using 2,3-dihydroxybiphenyl as a substrate. Activity toward substituted catechols was: 2,3-dihydroxybiphenyl > 3-methylcatechol > catechol > 4- ...	2012	21898128
resolution of 2-nitroalcohols by burkholderia cepacia lipase-catalyzed enantioselective acylation.	racemic 2-nitro-1-phenylethanol was resolved by via enantioselective transesterification catalyzed by burkholderia cepacia lipase. the reaction afforded excellent e values (e > 200) and enantioselectivity (up to >99% enantiomeric excesses [ee]) of both remaining substrates and acetylated product. moreover, the lipase displayed high enantioselectivity in the resolution of additional 2-nitroalcohols (e up to >200). this method provides an efficient alternative for obtaining enantiopure 2-nitroalco ...	2012	21972142
towards a rapid molecular diagnostic for melioidosis: comparison of dna extraction methods from clinical specimens.	optimising dna extraction from clinical samples for burkholderia pseudomallei type iii secretion system real-time pcr in suspected melioidosis patients confirmed that urine and sputum are useful diagnostic samples. direct testing on blood remains problematic; testing dna extracted from plasma was superior to dna from whole blood or buffy coat.	2012	22108495
screening of freeze-dried protective agents for the formulation of biocontrol strains, bacillus cereus ar156, burkholderia vietnamiensis b418 and pantoea agglomerans 2re40.	the effects of different freeze-drying protective agents on the viabilities of biocontrol strains bacillus cereus ar156, burkholderia vietnamiensis b418 and pantoea agglomerans 2re40 were investigated.	2012	22017666
antimicrobial and antibiofilm activity of ll-37 and its truncated variants against burkholderia pseudomallei.	the gram-negative bacterium burkholderia pseudomallei is the aetiological agent of melioidosis, which is an endemic disease in tropical areas of southeast asia and northern australia. burkholderia pseudomallei has intrinsic resistance to a number of commonly used antibiotics and has also been reported to develop a biofilm. resistance to killing by antimicrobial agents is one of the hallmarks of bacteria grown in biofilm. the aim of this study was to determine the antimicrobial activity and mecha ...	2012	22005071
In vitro activity of S-(3,4-dichlorobenzyl)isothiourea hydrochloride and novel structurally related compounds against multidrug-resistant bacteria, including Pseudomonas aeruginosa and Burkholderia cepacia complex.	The aim of this study was to establish the antimicrobial activities of S-(3,4-dichlorobenzyl)isothiourea hydrochloride (A22) and a series of structurally related compounds against multidrug-resistant (MDR) bacteria. The minimum inhibitory concentrations (MICs) of 21 compounds were determined against 18 strains of pathogenic bacteria in addition to Pseudomonas aeruginosa (n=19) and Burkholderia cepacia complex (BCC) (n=20) isolated from the sputa of cystic fibrosis patients. Selected compounds we ...	2012	21993484
in vitro antimicrobial studies of silver carbene complexes: activity of free and nanoparticle carbene formulations against clinical isolates of pathogenic bacteria.	silver carbenes may represent novel, broad-spectrum antimicrobial agents that have low toxicity while providing varying chemistry for targeted applications. here, the bactericidal activity of four silver carbene complexes (sccs) with different formulations, including nanoparticles (nps) and micelles, was tested against a panel of clinical strains of bacteria and fungi that are the causative agents of many skin and soft tissue, respiratory, wound, blood, and nosocomial infections.	2012	21972270
Regioselectivity-reversal in acylation of 6-azauridine catalyzed by Burkholderia cepacia lipase.	3'-O-Stearoylation of 6-azauridine was achieved enzymatically for the first time. Among eight commercially available lipases, that from Burkholderia cepacia displayed a 3'-regioselectivity of 80% towards the acylation of 3-hydroxyl of 6-azauridine. Using an immobilized lipase from Burkholderia cepacia, the 3'-regioselectivities of the acylations could be reversed by lengthening the aliphatic chain of the acyl donors (C2-C18). The possible reason might be the presence of the interaction between t ...	2012	21898129
myd88-dependent recruitment of monocytes and dendritic cells required for protection from pulmonary burkholderia mallei infection.	the gram-negative bacterium burkholderia mallei causes rapidly fatal illness in equines and humans when contracted by inhalation and also has the potential to be used as a bioweapon. however, little is known regarding the early innate immune responses and signaling mechanisms required to generate protection from pneumonic b. mallei infection. we showed previously that monocyte chemoattractant protein 1 (mcp-1) was a critical chemokine required for protection from pneumonic b. mallei infection. w ...	2012	22025508
is diversification history of maize influencing selection of soil bacteria by roots?	a wide range of plant lines has been propagated by farmers during crop selection and dissemination, but consequences of this crop diversification on plant-microbe interactions have been neglected. our hypothesis was that crop evolutionary history shaped the way the resulting lines interact with soil bacteria in their rhizospheres. here, the significance of maize diversification as a factor influencing selection of soil bacteria by seedling roots was assessed by comparing rhizobacterial community ...	2012	22126532
potato cytosine methylation and gene expression changes induced by a beneficial bacterial endophyte, burkholderia phytofirmans strain psjn.	burkholderia phytofirmans strain psjn is a highly effective plant-beneficial endophyte. we have used a combination of capillary electrophoresis and methylation-sensitive amplification length polymorphism (ce-msap) analysis to investigate the potato genomic dna cytosine methylation changes that occur in response to psjn bacterization. six weeks after psjn inoculation, over 6800 loci were identified and assessed in two in vitro grown potato varieties, the strongly-responsive red pontiac and the po ...	2012	22099516
[outbreak of burkholderia cepacia complex caused by contaminated alcohol-free mouthwash].	a total of three intensive care units (icu) at a german university hospital were involved in an outbreak of burkholderia cepacia complex (bcc).	2012	22273822
evaluation of the environmental specificity of fluorescence in situ hybridization (fish) using fluorescence-activated cell sorting (facs) of probe (pse1284)-positive cells extracted from rhizosphere soil.	we explicitly tested for the first time the 'environmental specificity' of traditional 16s rrna-targeted fluorescence in situ hybridization (fish) through comparison of the bacterial diversity actually targeted in the environment with the diversity that should be exactly targeted (i.e. without mismatches) according to in silico analysis. to do this, we exploited advances in modern flow cytometry that enabled improved detection and therefore sorting of sub-micron-sized particles and used probe ps ...	2012	22264503
heterocyclic urea derivatives and methods of use thereof (wo2010142978).	a series of ureido derivatives incorporating a highly derivatized quinolone scaffold were prepared by a succession of original steps. the lead molecules of these compounds are the quinolone antibacterials of the nalidixic acid/ciprofloxacin type and they target, similar to these clinically used agents, the dna gyrase/topoisomerase iv of pathogenic bacteria. these new quinolones are claimed to act as efficient antibacterials against a large number of drug-resistant bacteria such as staphylococcus ...	2012	22264034
bacterial taxa associated with the lung lichen lobaria pulmonaria are differentially shaped by geography and habitat.	the correlation between the taxonomic composition of alphaproteobacteria, burkholderia and nitrogen fixers associated with the lichen lobaria pulmonaria and the geographical distribution of the host was studied across four sites in europe. results proved that the diversity of alphaproteobacteria is affected by geography, while those of burkholderia and nitrogen fixers were mostly driven by local habitat. this difference indicates a higher stability of the association between alphaproteobacteria ...	2012	22268428
the genetic and molecular basis of o-antigenic diversity in burkholderia pseudomallei lipopolysaccharide.	lipopolysaccharide (lps) is one of the most important virulence and antigenic components of burkholderia pseudomallei, the causative agent of melioidosis. lps diversity in b. pseudomallei has been described as typical, atypical or rough, based upon banding patterns on sds-page. here, we studied the genetic and molecular basis of these phenotypic differences. bioinformatics was used to determine the diversity of genes known or predicted to be involved in biosynthesis of the o-antigenic moiety of ...	2012	22235357
characterisation of the burkholderia pseudomallei k96243 cps i coding region.	burkholderia pseudomallei is the causative agent of melioidosis, a disease endemic to regions of southeast asia and northern australia. both humans and a range of other animal species are susceptible to melioidosis and the production of a group 3 polysaccharide capsule in b. pseudomallei is essential for virulence. this b. pseudomallei capsular polysaccharide (cps) i comprises unbranched manno-heptopyranose residues and is encoded by a 34.5 kb locus on chromosome 1. despite the importance of thi ...	2012	22252864
burkholderia and cupriavidus spp. are the preferred symbionts of mimosa spp. in southern china.	rhizobia were isolated from invasive mimosa spp. (m. diplotricha and m. pudica) in dehong district of the province of yunnan in subtropical southern china. almost all of the 98 isolates were β-rhizobia in the genera burkholderia and cupriavidus. these strains were analyzed for their distribution characteristics together with strains from a previous study from sishuangbanna. the proportion of nodules containing each β-rhizobial genus varied between mimosa species, with cupriavidus being predomina ...	2012	22268711
extractive fermentation for enhanced production of thailandepsin a from burkholderia thailandensis e264 using polyaromatic adsorbent resin diaion hp-20.	thailandepsin a is natural product of burkholderia thailandensis e264 with potent histone deacetylase inhibitory activities and promising anticancer activities. the titer of thailandepsin a is very low (less than 10 mg/l) from limited empirical fermentation. to facilitate preclinical evaluations and potentially clinical development of thailandepsin a, systematic optimization and extractive fermentation of thailandepsin a from b. thailandensis e264 culture in flasks were investigated in this pilo ...	2012	22246221
new beta rhizobial strains able to efficiently nodulate parapiptadenia rigida (benth.) brenan.	among the leguminous trees natives to uruguay parapiptadenia rigida (angico), a mimosoideae legume, is one of the most promising species for agroforestry. like many other legumes, it is able to establish symbiotic associations with rhizobia and belongs to the group known as "nitrogen fixing trees", which are major components of agroforestry systems. information about rhizobial symbionts for this genus is scarce, and thus the aim of this work was to identify and characterize rhizobia associated w ...	2012	22226956
the burkholderia cenocepacia bdsf quorum sensing fatty acid is synthesized by a bifunctional crotonase homologue having both dehydratase and thioesterase activities.	signal molecules of the diffusible signal factor (dsf) family have been shown recently to be involved in regulation of pathogenesis and biofilm formation in diverse gram-negative bacteria. dsf signals are reported to be active not only on their cognate bacteria, but also on unrelated bacteria and the pathogenic yeast, candida albicans. dsfs are monounsaturated fatty acids of medium chain length containing an unusual cis-2 double bond. although genetic analyses had identified genes involved in ds ...	2012	22221091
Contemporary unconventional clinical use of co-trimoxazole.	Clin Microbiol Infect 2012; 18: 8-17 ABSTRACT: In the late 1960s, the combination of trimethoprim and sulphamethoxazole (co-trimoxazole) was introduced into clinical practice and used to treat many infectious diseases, such as urinary tract infections, respiratory infections, sexually transmitted diseases, Gram-negative sepsis, enteric infections and typhoid fever. Subsequently, co-trimoxazole was reported to be effective against numerous bacterial, fungal and protozoal pathogens, including Noca ...	2012	21851485
Identification of Hopanoid Biosynthesis Genes Involved in Polymyxin Resistance in Burkholderia multivorans.	A major challenge to clinical therapy of Burkholderia cepacia complex (Bcc) pulmonary infections is their innate resistance to a broad range of antimicrobials, including polycationic agents such as aminoglycosides, polymyxins, and cationic peptides. To identify genetic loci associated with this phenotype, a transposon mutant library was constructed in B. multivorans ATCC 17616 and screened for increased susceptibility to polymyxin B. Compared to the parent strain, mutant 26D7 exhibited 8- and 16 ...	2012	22006009
Proteomic analysis of colony morphology variants of Burkholderia pseudomallei defines a role for the arginine deiminase system in bacterial survival.	Colony morphology variation of Burkholderia pseudomallei is a notable feature of a proportion of primary clinical cultures from patients with melioidosis. Here, we examined the hypothesis that colony morphology switching results in phenotypic changes associated with enhanced survival under adverse conditions. We generated isogenic colony morphology types II and III from B. pseudomallei strain 153 type I, and compared their protein expression profiles using 2D gel electrophoresis. Numerous protei ...	2012	22062159
Expression of Aeromonas caviae polyhydroxyalkanoate synthase gene in Burkholderia sp. USM (JCM15050) enables the biosynthesis of SCL-MCL PHA from palm oil products.	Aims: Burkholderia sp. USM (JCM15050) isolated from oil-polluted wastewater is capable of utilizing palm oil products and glycerol to synthesize poly(3-hydroxybutyrate) [P(3HB)]. To confer the ability to produce polymer containing 3-hydroxyhexanoate (3HHx), plasmid (pBBREE32d13) harbouring the polyhydroxyalkanoate (PHA) synthase gene of Aeromonas caviae (phaC(Ac) ) was transformed into this strain. Methods and Results: The resulting transformant incorporated approximately 1 ± 0·3 mol% of 3HHx ...	2012	22054430
impact of conditioning films on the initial adhesion of burkholderia cepacia.	bacterial initial adhesion to inert surfaces in aquatic environments is mainly governed by the surface properties of the substratum, which can be altered significantly by the formation of conditioning films. bacteria were tested for ability to adhere to bare glass slides and to slides coated with alginate, bovine serum albumin (bsa), or suwannee river natural organic matter (sr-nom). three burkholderia cepacia strains with different extracellular polymeric substance (eps) secretion capacities we ...	2012	22112498