Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| [outbreak of burkholderia cepacia complex caused by contaminated alcohol-free mouthwash]. | a total of three intensive care units (icu) at a german university hospital were involved in an outbreak of burkholderia cepacia complex (bcc). | 2012 | 22273822 |
| burkholderia and cupriavidus spp. are the preferred symbionts of mimosa spp. in southern china. | rhizobia were isolated from invasive mimosa spp. (m. diplotricha and m. pudica) in dehong district of the province of yunnan in subtropical southern china. almost all of the 98 isolates were β-rhizobia in the genera burkholderia and cupriavidus. these strains were analyzed for their distribution characteristics together with strains from a previous study from sishuangbanna. the proportion of nodules containing each β-rhizobial genus varied between mimosa species, with cupriavidus being predomina ... | 2012 | 22268711 |
| bacterial taxa associated with the lung lichen lobaria pulmonaria are differentially shaped by geography and habitat. | the correlation between the taxonomic composition of alphaproteobacteria, burkholderia and nitrogen fixers associated with the lichen lobaria pulmonaria and the geographical distribution of the host was studied across four sites in europe. results proved that the diversity of alphaproteobacteria is affected by geography, while those of burkholderia and nitrogen fixers were mostly driven by local habitat. this difference indicates a higher stability of the association between alphaproteobacteria ... | 2012 | 22268428 |
| evaluation of the environmental specificity of fluorescence in situ hybridization (fish) using fluorescence-activated cell sorting (facs) of probe (pse1284)-positive cells extracted from rhizosphere soil. | we explicitly tested for the first time the 'environmental specificity' of traditional 16s rrna-targeted fluorescence in situ hybridization (fish) through comparison of the bacterial diversity actually targeted in the environment with the diversity that should be exactly targeted (i.e. without mismatches) according to in silico analysis. to do this, we exploited advances in modern flow cytometry that enabled improved detection and therefore sorting of sub-micron-sized particles and used probe ps ... | 2012 | 22264503 |
| heterocyclic urea derivatives and methods of use thereof (wo2010142978). | a series of ureido derivatives incorporating a highly derivatized quinolone scaffold were prepared by a succession of original steps. the lead molecules of these compounds are the quinolone antibacterials of the nalidixic acid/ciprofloxacin type and they target, similar to these clinically used agents, the dna gyrase/topoisomerase iv of pathogenic bacteria. these new quinolones are claimed to act as efficient antibacterials against a large number of drug-resistant bacteria such as staphylococcus ... | 2012 | 22264034 |
| characterisation of the burkholderia pseudomallei k96243 cps i coding region. | burkholderia pseudomallei is the causative agent of melioidosis, a disease endemic to regions of southeast asia and northern australia. both humans and a range of other animal species are susceptible to melioidosis and the production of a group 3 polysaccharide capsule in b. pseudomallei is essential for virulence. this b. pseudomallei capsular polysaccharide (cps) i comprises unbranched manno-heptopyranose residues and is encoded by a 34.5 kb locus on chromosome 1. despite the importance of thi ... | 2012 | 22252864 |
| extractive fermentation for enhanced production of thailandepsin a from burkholderia thailandensis e264 using polyaromatic adsorbent resin diaion hp-20. | thailandepsin a is natural product of burkholderia thailandensis e264 with potent histone deacetylase inhibitory activities and promising anticancer activities. the titer of thailandepsin a is very low (less than 10 mg/l) from limited empirical fermentation. to facilitate preclinical evaluations and potentially clinical development of thailandepsin a, systematic optimization and extractive fermentation of thailandepsin a from b. thailandensis e264 culture in flasks were investigated in this pilo ... | 2012 | 22246221 |
| the genetic and molecular basis of o-antigenic diversity in burkholderia pseudomallei lipopolysaccharide. | lipopolysaccharide (lps) is one of the most important virulence and antigenic components of burkholderia pseudomallei, the causative agent of melioidosis. lps diversity in b. pseudomallei has been described as typical, atypical or rough, based upon banding patterns on sds-page. here, we studied the genetic and molecular basis of these phenotypic differences. bioinformatics was used to determine the diversity of genes known or predicted to be involved in biosynthesis of the o-antigenic moiety of ... | 2012 | 22235357 |
| new beta rhizobial strains able to efficiently nodulate parapiptadenia rigida (benth.) brenan. | among the leguminous trees natives to uruguay parapiptadenia rigida (angico), a mimosoideae legume, is one of the most promising species for agroforestry. like many other legumes, it is able to establish symbiotic associations with rhizobia and belongs to the group known as "nitrogen fixing trees", which are major components of agroforestry systems. information about rhizobial symbionts for this genus is scarce, and thus the aim of this work was to identify and characterize rhizobia associated w ... | 2012 | 22226956 |
| structural analysis of capsular polysaccharides expressed by burkholderia mallei and burkholderia pseudomallei. | capsular polysaccharides (cpss) were isolated from o-polysaccharide deficient strains of burkholderia mallei and burkholderia pseudomallei using a modified hot phenol/water extraction procedure. glycosyl composition, methylation, maldi-tof ms analyses as well as (1)h nmr spectroscopy including cosy, tocsy, noesy, hmbc and hsqc experiments identified the presence of two distinct cps antigens in the samples exhibiting the following structures: this study confirms the ability of b. mallei to expres ... | 2011 | 22221792 |
| the burkholderia cenocepacia bdsf quorum sensing fatty acid is synthesized by a bifunctional crotonase homologue having both dehydratase and thioesterase activities. | signal molecules of the diffusible signal factor (dsf) family have been shown recently to be involved in regulation of pathogenesis and biofilm formation in diverse gram-negative bacteria. dsf signals are reported to be active not only on their cognate bacteria, but also on unrelated bacteria and the pathogenic yeast, candida albicans. dsfs are monounsaturated fatty acids of medium chain length containing an unusual cis-2 double bond. although genetic analyses had identified genes involved in ds ... | 2012 | 22221091 |
| thirty years of heme catalases structural biology. | about thirty years ago the crystal structures of the heme catalases from penicillium vitale (pvc) and, a few months later, from bovine liver (blc) were published. both enzymes were compact tetrameric molecules with subunits that, despite their size differences and the large phylogenetic separation between the two organisms, presented a striking structural similarity for about 460 residues. the high conservation, confirmed in all the subsequent structures determined, suggested a strong pressure t ... | 2011 | 22209752 |
| Synthesis of the trisaccharide outer core fragment of Burkholderia cepacia pv. vietnamiensis lipooligosaccharide. | The synthesis of ß-Gal-(1?3)-a-GalNAc-(1?3)-ß-GalNAc allyl trisaccharide as the outer core fragment of Burkholderia cepacia pv. vietnamiensis lipooligosaccharide was accomplished through a concise, optimized, multi-step synthesis, having as key steps three glycosylations, that were in-depth studied performing them under several conditions. The target trisaccharide was designed with an allyl aglycone in order to open a future access to the conjugation with an immunogenic protein en route to the d ... | 2011 | 22209378 |
| Identification, origin, and evolution of leaf nodulating symbionts of Sericanthe (Rubiaceae). | Bacterial leaf symbiosis is an intimate association between bacteria and plants in which endosymbionts are housed within leaf nodules. This phenomenon has been reported in three genera of Rubiaceae (Pavetta, Psychotria, and Sericanthe), but the bacterial partner has only been identified in Psychotria and Pavetta. Here we report the identification of symbiotic bacteria in two leaf nodulating Sericanthe species. Using 16S rRNA data and common housekeeping genetic markers (recA and gyrB) we studied ... | 2011 | 22203556 |
| Cupriavidus and Burkholderia species associated with agricultural plants that grow in alkaline soils. | The presence of Burkholderia, Cupriavidus, and Ralstonia species in northeastern Mexico was investigated. An analysis of the root surrounding soil from different agricultural plants led to the isolation of Burkholderia and Cupriavidus species but no Ralstonia strains. Most Cupriavidus species were unknown and grouped into two clusters according to ARDRA profiles. The 16S rRNA sequence analysis showed that the Cupriavidus isolates were highly related among them and with different Cupriavidus spec ... | 2011 | 22203548 |
| microvirga lupini sp. nov., microvirga lotononidis sp. nov., and microvirga zambiensis sp. nov. are alphaproteobacterial root nodule bacteria that specifically nodulate and fix nitrogen with geographically and taxonomically separate legume hosts. | strains of gram-negative, rod-shaped, non-spore-forming bacteria were isolated from nitrogen-fixing nodules of the native legumes listia angolensis (from zambia) and lupinus texensis (from texas, usa). phylogenetic analysis of the 16s rrna gene showed that the novel strains belong to the genus microvirga, with 96.1 % or greater sequence similarity with type strains of this genus. the closest relative of the representative strains lut6t and wsm3557t was m. flocculans tfbt, with 97.6-98.0 % simila ... | 2011 | 22199210 |
| diversity of 16s-23s rdna internal transcribed spacer (its) reveals phylogenetic relationships in burkholderia pseudomallei and its near-neighbors. | length polymorphisms within the 16s-23s ribosomal dna internal transcribed spacer (its) have been described as stable genetic markers for studying bacterial phylogenetics. in this study, we used these genetic markers to investigate phylogenetic relationships in burkholderia pseudomallei and its near-relative species. b. pseudomallei is known as one of the most genetically recombined bacterial species. in silico analysis of multiple b. pseudomallei genomes revealed approximately four homologous r ... | 2011 | 22195045 |
| Bacterial communities in urban aerosols collected with wetted-wall cyclonic samplers and seasonal fluctuations of live and culturable airborne bacteria. | Airborne transmission of bacterial pathogens from point sources (e.g., ranches, dairy waste treatment facilities) to areas of food production (farms) has been suspected. Determining the incidence, transport and viability of extremely low levels of pathogens require collection of high volumes of air and characterization of live bacteria from aerosols. We monitored the numbers of culturable bacteria in urban aerosols on 21 separate days during a 9 month period using high volume cyclonic samplers a ... | 2011 | 22193549 |
| microbiological spectrum of brain abscess at a tertiary care hospital in south india: 24-year data and review. | intracranial abscesses are life-threatening infections that pose a diagnostic challenge not only to the neurosurgeon but also to the microbiologists. detailed studies documenting the spectrum of infecting agents involved in brain abscesses are limited from india. materials and methods. this is a retrospective analysis of 352 samples from 1987 to 2010 analyzed at a tertiary care hospital in south india from 1987 to 2010, to document the changing trends with time. results. the age of the patients ... | 2011 | 22191080 |
| the microbial ecology of wine grape berries. | grapes have a complex microbial ecology including filamentous fungi, yeasts and bacteria with different physiological characteristics and effects upon wine production. some species are only found in grapes, such as parasitic fungi and environmental bacteria, while others have the ability to survive and grow in wines, constituting the wine microbial consortium. this consortium covers yeast species, lactic acid bacteria and acetic acid bacteria. the proportion of these microorganisms depends on th ... | 2011 | 22189021 |
| Proteomics Analyses of the Opportunistic Pathogen Burkholderia vietnamiensis Using Protein Fractionations and Mass Spectrometry. | The main objectives of this work were to obtain a more extensive coverage of the Burkholderia vietnamiensis proteome than previously reported and to identify virulence factors using tandem mass spectrometry. The proteome of B. vietnamiensis was precipitated into four fractions to as extracellular, intracellular, cell surface and cell wall proteins. Two different approaches were used to analyze the proteins. The first was a gel-based method where 1D SDS-PAGE was used for separation of the protein ... | 2011 | 22187530 |
| [Detection and identification of highly pathogenic bacteria within the framework of the EQADeBa project--Part I: Samples containing living pathogens]. | Bacillus anthracis, Yersinia pestis, Francisella tularensis, Brucella sp., Bulkholderia mallei are B. pseudomallei are highly pathogenic bacteria of potential bioterrorism risk. To support the early warning and rapid response capacity to ensure an effective reaction to bioterrorist attacks the international project "Establishment of Quality Assurances for Detection of Highly Pathogenic Bacteria of Potential Bioterrorism Risk" (EQADeBa) has been established. The aim of the project was establishme ... | 2011 | 22184939 |
| diagnostic assays for glanders. | 2011 | 22184353 | |
| epidemiological tracking and population assignment of the non-clonal bacterium, burkholderia pseudomallei. | rapid assignment of bacterial pathogens into predefined populations is an important first step for epidemiological tracking. for clonal species, a single allele can theoretically define a population. for non-clonal species such as burkholderia pseudomallei, however, shared allelic states between distantly related isolates make it more difficult to identify population defining characteristics. two distinct b. pseudomallei populations have been previously identified using multilocus sequence typin ... | 2011 | 22180792 |
| Regulation of Universal Stress Protein Genes by Quorum Sensing and RpoS, and Its Role in Burkholderia glumae. | Burkholderia glumae possesses a quorum sensing (QS) system mediated by N-octanoyl-homoserine lactone (C8-HSL) and its cognate receptor TofR. TofR/C8-HSL regulates expression of a transcriptional regulator, qsmR. We identified one of the universal stress proteins (Usps), Usp2, from the genome-wide analysis of QS-dependent proteomes of B. glumae. In the whole genome of B. glumae BGR1, 11 usp genes (usp1 to 11) were identified. Among the stress conditions tested, usp1 and usp2 mutants died 1 h afte ... | 2011 | 22178971 |
| out of the ground: aerial and exotic habitats of the melioidosis bacterium burkholderia pseudomallei in grasses in australia. | melioidosis is an emerging infectious disease of humans and animals in the tropics caused by the soil bacterium burkholderia pseudomallei. despite high fatality rates, the ecology of b. pseudomallei remains unclear. we used a combination of field and laboratory studies to investigate b. pseudomallei colonization of native and exotic grasses in northern australia. multivariable and spatial analyses were performed to determine significant predictors for b. pseudomallei occurrence in plants and soi ... | 2011 | 22176696 |
| bioremediation of carbofuran contaminated soil under saturated condition: soil column study. | disturbed soil columns, 5.8 cm in diameter and 25 cm in length, were used as a basic model to simulate the movement of carbofuran in rice field soil under saturated conditions. bioaugmentation using a specific carbofuran degrader, burkholderia sp. pcl3, in free and immobilized cell forms and biostimulation using rice straw as organic amendment were applied with the aim of enhancing the degradation of carbofuran in soil and to prevent the movement of carbofuran along with the flow through. in the ... | 2011 | 22173656 |
| [Burkholderia cepacia bacteremia: a prospective analysis of 33 episodes]. | Introduction: The aim of this study is to describe clinical characteristics and outcome of Burkholderia cepacia bacteraemia, susceptibility of the isolates and differences between cases from epidemic outbreaks and sporadic cases. Material and methods: From 1993 to 2009, episodes of B. cepacia bacteraemia were prospectively collected in a university hospital. Results: A total of 33 episodes were included, of which 21 were part of two outbreaks (9 in 1994 and 12 in 2006). Outbreak cases had a med ... | 2011 | 22173191 |
| identification of differentially expressed proteins from burkholderia pseudomallei isolated during primary and relapsing melioidosis. | burkholderia pseudomallei causes septicemic melioidosis with a high rate of relapse, however microbial determinants of relapse are unknown. proteins were analyzed from sequential b. pseudomallei isolates from primary and relapsing melioidosis. analysis by isotope tagging for relative and absolute quantitation revealed that factors required for nitric oxide detoxification (hmpa) and necessary for anaerobic growth (arca, arcc and arcb) were highly expressed in the relapse isolate. two-dimensional ... | 2011 | 22172334 |
| Screening microorganisms for insulin binding reveals binding by Burkholderia multivorans and Burkholderia cenocepacia and novel attachment of insulin to Aeromonas salmonicida via the A-layer. | Exposure to microorganisms is considered an environmental factor which can contribute to Type 1 diabetes. Insulin binding proteins on microorganisms may induce production of antibodies which can react with the human insulin receptor (HIR) with possible consequences in developing a diabetic autoimmune response against HIR and insulin. The interaction of insulin with microorganisms was studied by screening forty-five microbial species for their ability to bind insulin. Binding assays were performe ... | 2011 | 22171975 |
| exposing the third chromosome of burkholderia cepacia complex strains as a virulence plasmid. | the burkholderia cepacia complex (bcc) consists of 17 closely related species of opportunistic bacterial pathogens, which are particularly problematic for cystic fibrosis patients and immunocompromised individuals. bcc genomes consist of multiple replicons, and each strain sequenced to date has three chromosomes. in addition to genes thought to be essential for survival, each chromosome carries at least one rrna operon. we isolated three mutants during a transposon mutagenesis screen that were n ... | 2011 | 22171913 |
| the fucose-binding lectin from opportunistic pathogen burkholderia ambifaria binds to both plant and human oligosaccharidic epitopes. | burkholderia ambifaria is generally associated with the rhizosphere of plants where it has biocontrol effects on other microorganisms. it is also a member of the burkhoderia cepacia complex, a group of closely related bacteria that causes lung infections in immunocompromised patients, as well as patients with granulomatous disease or cystic fibrosis. our previous work indicated that fucose on human epithelia is a frequent target for lectins and adhesins of lung pathogens (sulak et al., plos path ... | 2011 | 22170069 |
| Role of NADPH Oxidase versus Neutrophil Proteases in Antimicrobial Host Defense. | NADPH oxidase is a crucial enzyme in mediating antimicrobial host defense and in regulating inflammation. Patients with chronic granulomatous disease, an inherited disorder of NADPH oxidase in which phagocytes are defective in generation of reactive oxidant intermediates (ROIs), suffer from life-threatening bacterial and fungal infections. The mechanisms by which NADPH oxidase mediate host defense are unclear. In addition to ROI generation, neutrophil NADPH oxidase activation is linked to the re ... | 2011 | 22163282 |
| polyphasic approach for the characterization of rhizobial symbionts effective in fixing n(2) with common bean (phaseolus vulgaris l.). | common bean (phaseolus vulgaris l.) is a legume that has been reported as highly promiscuous in nodulating with a variety of rhizobial strains, often with low effectiveness in fixing nitrogen. the aim of this work was to assess the symbiotic efficiency of rhizobial strains isolated from common bean seeds, nodules of arachis hypogaea, mucuna pruriens, and soils from various brazilian agroecosystems, followed by the characterization of elite strains identified in the first screening. forty-five el ... | 2011 | 22159885 |
| adaptation mechanisms of bacteria during the degradation of polychlorinated biphenyls in the presence of natural and synthetic terpenes as potential degradation inducers. | in this study, we examined the effect of polychlorinated biphenyls (pcbs) in the presence of natural and synthetic terpenes and biphenyl on biomass production, lipid accumulation, and membrane adaptation mechanisms of two pcb-degrading bacterial strains pseudomonas stutzeri and burkholderia xenovorans lb400. according to the results obtained, it could be concluded that natural terpenes, mainly those contained in ivy leaves and pine needles, decreased adaptation responses induced by pcbs in these ... | 2011 | 22159613 |
| the antibacterial properties of docosahexaenoic omega-3 fatty acid against the cystic fibrosis multi-resistant pathogen burkholderia cenocepacia. | burkholderia cepacia complex (bcc) bacteria are opportunistic pathogens that cause multiresistant pulmonary infections in cystic fibrosis (cf) patients. in this study we evaluated the in vitro antimicrobial efficacy of eight unsaturated fatty acids against burkholderia cenocepacia k56-2, a cf epidemic strain. docosahexaenoic acid (dha) was the most active compound. its action can be either bacteriostatic or bactericidal, depending upon the concentration used. the effect of dha was also evaluated ... | 2011 | 22150831 |
| Antioxidant compounds improved PCB-degradation by Burkholderia xenovorans strain LB400. | Polychlorobiphenyls (PCBs) are toxic and persistent organic pollutants that are widely distributed in the environment. Burkholderia xenovorans LB400 is capable of degrading aerobically an unusually wide range of PCBs. However, during PCB-degradation B. xenovorans LB400 generates reactive oxygen species (ROS) that affect its viability. The aim of this study was to increase the efficiency of PCB-degradation of B. xenovorans LB400 by adding antioxidant compounds that could increase tolerance to oxi ... | 2011 | 22142725 |
| Isolation and characterization of monochloroacetic acid-degrading bacteria. | Five Burkholderia strains (CL-1, CL-2, CL-3, CL-4, and CL-5) capable of degrading monochloroacetic acid (MCA) were isolated from activated sludge or soil samples gathered from several parts of Japan. All five isolates were able to grow on MCA as the sole source of carbon and energy, and argentometry and gas chromatography-mass spectroscopy analyses showed that these five strains consumed MCA completely and released chloride ions stoichiometrically within 25 h. The five isolates also grew on mono ... | 2011 | 22139456 |
| cloning, purification and crystallographic analysis of a hypothetical protein, bpsl1549, from burkholderia pseudomallei. | burkholderia pseudomallei bpsl1549, a putative protein of unknown function, has been overexpressed in escherichia coli, purified and subsequently crystallized by the hanging-drop vapour-diffusion method using peg as a precipitant to give crystals with overall dimensions of 0.15 × 0.15 × 0.1 mm. native data were collected to 1.47 å resolution at the european synchrotron radiation facility (esrf). the crystals belonged to space group p2(1)2(1)2(1), with unit-cell parameters a = 37.1, b = 45.4, c = ... | 2011 | 22139182 |
| Characterization of DC1, a broad host range Bcep22-like podovirus. | Bcep22-like phages are a recently described group of podoviruses that infect strains of Burkholderia cenocepacia. We have isolated and characterized a novel member of this group named DC1. This podovirus shows many genomic similarities to BcepIL02 and Bcep22, but infects strains belonging to multiple Burkholderia cepacia complex (BCC) species. | 2011 | 22139000 |
| delayed activation of host innate immune pathways in streptozotocin-induced diabetic hosts leads to more severe disease during infection with burkholderia pseudomallei. | diabetes mellitus is a predisposing factor of melioidosis contributing to higher mortality rates in diabetics infected with burkholderia pseudomallei. to investigate how diabetes alters the inflammatory response, we established a streptozotocin (stz)-induced diabetic murine acute-phase melioidosis model. viable b. pseudomallei cells were consistently detected in the blood, liver and spleen during the 42 hr course of infection but the hyperglycemic environment did not increase the bacterial burde ... | 2011 | 22136109 |
| Bacterial cis-2-unsaturated fatty acids found in the cystic fibrosis airway modulate virulence and persistence of Pseudomonas aeruginosa. | There is an increasing appreciation of the polymicrobial nature of many bacterial infections such as those associated with cystic fibrosis (CF) and of the potentially important role for interspecies interactions in influencing both bacterial virulence and response to therapy. Patients with CF are often co-infected with Pseudomonas aeruginosa and other pathogens including Burkholderia cenocepacia and Stenotrophomonas maltophilia. These latter bacteria produce signal molecules of the diffusible si ... | 2011 | 22134647 |
| Study of the effect of DNA polymorphisms in the mannose-binding lectin gene (MBL2) on disease severity in Slovak cystic fibrosis patients. | Lung infections are the leading cause of morbidity and mortality in cystic fibrosis (CF). Mannose-binding lectin (MBL) is a key factor in innate immunity. We therefore investigated whether MBL2 gene variants are associated with pulmonary function or susceptibility to Pseudomonas aeruginosa and Burkholderia cepacia infection in Slovak patients affected with CF. DNA polymorphisms in exon 1 and the promoter region were typed by single base primer extension assay in 91 patients and 100 healthy contr ... | 2011 | 22131319 |
| immunogenic consensus sequence t helper epitopes for a pan-burkholderia biodefense vaccine. | background. biodefense vaccines against category b bioterror agents burkholderia pseudomallei (bpm) and burkholderia mallei (bm) are needed, as they are both easily accessible to terrorists and have strong weaponization potential. burkholderia cepaciae (bc), a related pathogen, causes chronic lung infections in cystic fibrosis patients. since bpm, bm and bc are all intracellular bacteria, they are excellent targets for t cell-based vaccines. however, the sheer volume of available genomic data re ... | 2011 | 22130150 |
| burkholderia pseudomallei: an uncommon cause of bacteraemic pneumonia in a diabetic. | 2011 | 22128627 | |
| is diversification history of maize influencing selection of soil bacteria by roots? | a wide range of plant lines has been propagated by farmers during crop selection and dissemination, but consequences of this crop diversification on plant-microbe interactions have been neglected. our hypothesis was that crop evolutionary history shaped the way the resulting lines interact with soil bacteria in their rhizospheres. here, the significance of maize diversification as a factor influencing selection of soil bacteria by seedling roots was assessed by comparing rhizobacterial community ... | 2012 | 22126532 |
| protective antigens against glanders identified by expression library immunization. | burkholderia are highly evolved gram-negative bacteria that primarily infect solipeds but are transmitted to humans by ingestion and cutaneous or aerosol exposures. heightened concern over human infections of burkholderia mallei and the very closely related species b. pseudomallei is due to the pathogens' proven effectiveness as bioweapons, and to the increased potential for natural opportunistic infections in the growing diabetic and immuno-compromised populations. these burkholderia species ar ... | 2011 | 22125550 |
| reflectron maldi tof and maldi tof/tof mass spectrometry reveal novel structural details of native lipooligosaccharides. | lipooligosaccharides (los) are powerful gram-negative glycolipids that evade the immune system and invade host animal and vegetal cells. the structural elucidation of los is pivotal to understanding the mechanisms of infection at the molecular level. the amphiphilic nature of los has been the main obstacle for structural analysis by matrix-assisted laser desorption/ionization (maldi) mass spectrometry (ms). our approach has resolved this important issue and has permitted us to obtain reflectron ... | 2011 | 22124985 |
| In Vitro Antibacterial Activity of Sphaeropsidins and Chemical Derivatives toward Xanthomonas oryzae pv. oryzae, the Causal Agent of Rice Bacterial Blight. | Sphaeropsidin A, the main phytotoxin produced by Diplodia cupressi, as well as the two natural analogues sphaeropsidins B and C and 14 derivatives obtained by chemical modifications were assayed for antibacterial activity against Xanthomonas oryzae pv. oryzae, Pseudomonas fuscovaginae, and Burkholderia glumae, the causal agents of severe bacterial rice diseases. The results showed a strong and specific activity of sphaeropsidin A against X. oryzae pv. oryzae, while no activity was observed again ... | 2011 | 22124378 |
| development of an acute model of inhalational melioidosis in the common marmoset (callithrix jacchus). | studies of inhalational melioidosis were undertaken in the common marmoset (callithrix jacchus). following exposure to an inhaled challenge with aerosolized burkholderia pseudomallei, lethal infection was observed in marmosets challenged with doses below 10 cfu; a precise ld(50) determination was not possible. the model was further characterized using a target challenge dose of approximately 10(2) cfu. a separate pathogenesis time-course experiment was also conducted. all animals succumbed, betw ... | 2011 | 22122591 |
| Microwave-assisted enzymatic synthesis of beef tallow biodiesel. | Optimal conditions for the microwave-assisted enzymatic synthesis of biodiesel have been developed by a full 2(2) factorial design leading to a set of seven runs with different combinations of molar ratio and temperature. The main goal was to reduce the reaction time preliminarily established by a process of conventional heating. Reactions yielding biodiesel, in which beef tallow and ethanol used as raw materials were catalyzed by lipase from Burkholderia cepacia immobilized on silica-PVA and mi ... | 2011 | 22120648 |
| chance and necessity in the evolution of a bacterial pathogen. | the combination of genomic, epidemiological and evolutionary analyses provides a powerful toolbox for understanding how pathogens adapt to their human hosts. by sequencing 112 burkholderia dolosa genomes from an outbreak among patients with cystic fibrosis, a new study documents evolution in action and identifies a set of genes that contributed to the pathogen's adaptation. | 2011 | 22120052 |
| intravenous immunoglobulin treatment for macrophage activation syndrome complicating chronic granulomatous disease. | objectives: chronic granulomatous disease is a rare phagocyte disorder characterized by an increased susceptibility to infections and inflammatory complications. we describe two patients with chronic granulomatous disease (cgd) complicated by macrophage activation syndrome (mas) (secondary hemophagocytic lymphohistiocytosis) treated with intravenous immunoglobulin (ivig). methods: a report of two cases of cgd com ... | 2011 | 22119934 |
| discovery of dna operators for tetr and marr family transcription factors fromburkholderia xenovorans. | determining transcription factor recognition motifs or operator sites is central to understanding gene regulation, yet few operators have been characterized. in this study, we used a protein-binding microarray (pbm) to discover the dna recognition sites and putative regulons for three tetr and one marr family tfs derived from b. xenovorans, which are common to the burkholderia genus. we also describe the development and application of a more streamlined version of the pbm technology that signifi ... | 2011 | 22117006 |
| Structural characterization of two lipopolysaccharide O-antigens produced by the endofungal bacterium Burkholderia sp. HKI-402 (B4). | Two different polysaccharides were isolated and identified from the lipopolysaccharide fraction of endofungal bacterium Burkholderia sp. HKI-402 (B4). The complete structure was elucidated by chemical analysis and 2D NMR spectroscopy as the following: | 2011 | 22115719 |
| diagnostic use of burkholderia pseudomallei selective media in a low prevalence setting. | routine use of selective media improves diagnosis of burkholderia pseudomallei, but resources may be limited in endemic developing countries. to maximise yield in the relatively low-prevalence setting of kuala lumpur, malaysia, b. pseudomallei selective agar and broth were compared with routine media for 154 respiratory specimens from patients with community-acquired disease. selective media detected three additional culture-positive specimens and one additional melioidosis patient, at a consuma ... | 2011 | 22112687 |
| impact of conditioning films on the initial adhesion of burkholderia cepacia. | bacterial initial adhesion to inert surfaces in aquatic environments is mainly governed by the surface properties of the substratum, which can be altered significantly by the formation of conditioning films. bacteria were tested for ability to adhere to bare glass slides and to slides coated with alginate, bovine serum albumin (bsa), or suwannee river natural organic matter (sr-nom). three burkholderia cepacia strains with different extracellular polymeric substance (eps) secretion capacities we ... | 2012 | 22112498 |
| Successful Cessation of Transmitting Healthcare-Associated Infections due to Burkholderia cepacia Complex in a Neonatal Intensive Care Unit in a Japanese Children's Hospital. | Background: Burkholderia cepacia strains have been known to possess the capability to cause serious infections especially in neonatal intensive care units (NICUs), and their multi-drug resistances become a severe threat in hospital settings. The aim of this investigation was to evaluate the B. cepacia complex infections in the NICU in Nagano Children's Hospital, Azumino 399-8288, Japan, and to report the intervention leading to the successful cessation of the outbreak. - Methodology: The inciden ... | 2011 | 22112360 |
| towards a rapid molecular diagnostic for melioidosis: comparison of dna extraction methods from clinical specimens. | optimising dna extraction from clinical samples for burkholderia pseudomallei type iii secretion system real-time pcr in suspected melioidosis patients confirmed that urine and sputum are useful diagnostic samples. direct testing on blood remains problematic; testing dna extracted from plasma was superior to dna from whole blood or buffy coat. | 2012 | 22108495 |
| Occidiofungin's Chemical Stability and In vitro Potency Against Candida species. | Occidiofungin is a cyclic glyco-lipopeptide produced by Burkholderia contaminans. Minimum inhibitory concentrations (MICs) against Candida species were between 0.5 and 1.0 µg/mL. Occidiofungin retains its in vitro potency in the presence of 5% and 50% human serum with a minimal lethal concentration (MLC) of 2 and 4 µg/mL, respectively. Time-kill and post-antifungal effect (PAFE) experiments of occidiofungin against Candida albicans were measured. These results demonstrate that occidiofungin is f ... | 2011 | 22106210 |
| Genetic Diversity of Endophytic Diazotrophs of the Wild Rice, Oryza alta and Identification of the New Diazotroph, Acinetobacter oryzae sp. nov. | Thirty-three endophytic diazotrophs were isolated from surface-sterilized leaves, stem, and roots of wild rice Oryza alta. The SDS-PAGE profile of total protein and insertion sequence-based polymerase chain reaction (IS-PCR) fingerprinting grouped the isolates into four clusters (I-IV). The 16S rRNA gene sequence homology of the representative strains B21, B31, B1, and B23 of clusters I, II, III, and IV were assigned to Pseudomonas oleovorans (99.2% similarity), Burkholderia fungorum (99.4% simi ... | 2011 | 22105517 |
| enantiomeric scaffolding of α-tetralone and related scaffolds by ekr (enzymatic kinetic resolution) and stereoselective ketoreduction with ketoreductases. | stereochemically pure compounds containing an all carbon quaternary stereocenter based on 1-tetralone, 1-indanone and 4-chromanone scaffolds have been synthesized by employing lipase ps (burkholderia cepacia) catalyzed kinetic resolution. these scaffolds are further functionalized by microbial ketoreductase enzymes (geotrichum candidum, candida parapsilosis and aspergillus niger) to access stereochemically pure diols which, on further synthetic manipulation, yield novel cyclic compounds. | 2011 | 22102058 |
| Structures of Burkholderia thailandensis nucleoside kinase: implications for the catalytic mechanism and nucleoside selectivity. | The nucleoside kinase (NK) from the mesophilic Gram-negative bacterium Burkholderia thailandensis (BthNK) is a member of the phosphofructokinase B (Pfk-B) family and catalyzes the Mg(2+)- and ATP-dependent phosphorylation of a broad range of nucleosides such as inosine (INO), adenosine (ADO) and mizoribine (MZR). BthNK is currently used in clinical practice to measure serum MZR levels. Here, crystal structures of BthNK in a ligand-free form and in complexes with INO, INO-ADP, MZR-ADP and AMP-Mg( ... | 2011 | 22101821 |
| 2,4-DNT removal in intimately coupled photobiocatalysis: the roles of adsorption, photolysis, photocatalysis, and biotransformation. | The removal of 2,4-dinitrotoluene (2,4-DNT) by simultaneous UV-photo(cata)lysis and biodegradation was explored using intimately coupled photolysis/photocatalysis and biodegradation (ICPB) with two novel porous carriers. First, a porous ceramic carrier was used to attach the photocatalyst (TiO(2)) on its exterior and accumulate biomass in its interior. UV irradiation alone decomposed 71% of the 2,4-DNT in 60 h, and TiO(2) catalyst improved the photolysis to 77%. Second, a macroporous sponge carr ... | 2011 | 22101782 |
| effectiveness of a simplified method for isolation of burkholderia pseudomallei from soil. | detection of environmental burkholderia pseudomallei indicates a risk for melioidosis and is important for the development of a global risk map. we describe a simple method for detecting b. pseudomallei using direct culture of soil in enrichment broth. this gives a rate of positivity comparable to that obtained with a standard method but is cheaper and labor saving. | 2011 | 22101048 |
| potato cytosine methylation and gene expression changes induced by a beneficial bacterial endophyte, burkholderia phytofirmans strain psjn. | burkholderia phytofirmans strain psjn is a highly effective plant-beneficial endophyte. we have used a combination of capillary electrophoresis and methylation-sensitive amplification length polymorphism (ce-msap) analysis to investigate the potato genomic dna cytosine methylation changes that occur in response to psjn bacterization. six weeks after psjn inoculation, over 6800 loci were identified and assessed in two in vitro grown potato varieties, the strongly-responsive red pontiac and the po ... | 2012 | 22099516 |
| antibiotic resistance and extended-spectrum β-lactamases in isolated bacteria from seawater of algiers beaches (algeria). | the aim of the study was to evaluate bacterial antibiotic resistance in seawater from four beaches in algiers. the most significant resistance rates were observed for amoxicillin and ticarcillin, whereas they were relatively low for ceftazidime, cefotaxime and imipenem. according to sampling sites, the highest resistance rates were recorded for 2 sites subjected to chemical and microbiological inputs (amoxicillin, 43% and 52%; ticarcillin, 19.6% and 47.7%), and for 2 sites relatively preserved f ... | 2011 | 22095134 |
| genetic diversity of mimosa pudica rhizobial symbionts in soils of french guiana: investigating the origin and diversity of burkholderia phymatum and other beta-rhizobia. | the genetic diversity of 221 mimosa pudica bacterial symbionts trapped from eight soils from diverse environments in french guiana was assessed by 16s rrna pcr-rflp, rep-pcr fingerprints, as well as by phylogenies of their 16s rrna and reca housekeeping genes, and by their nifh, noda and nodc symbiotic genes. interestingly, we found a large diversity of beta-rhizobia, with burkholderia phymatum and burkholderia tuberum being the most frequent and diverse symbiotic species. other species were als ... | 2011 | 22093060 |
| bopc is a type iii secreted effector protein of burkholderia pseudomallei. | burkholderia pseudomallei, the causative agent of melioidosis, exploits the bsa type iii secretion system (t3ss) to deliver effector proteins into host cells. these effectors manipulate host cell functions; thus, contributing to the ability of the bacteria to evade the immune response and cause disease. only two bsa-secreted effectors have been conclusively identified to date. here, we report the identification of the third b. pseudomallei type iii secreted effector protein, designated bopc. bop ... | 2011 | 22092682 |
| Total Synthesis and Stereochemical Assignment of Burkholdac B, a Depsipeptide HDAC Inhibitor. | Three diastereomers of burkholdac B were prepared by total synthesis, enabling the full stereochemical assignment of the natural product. It is proposed that burkholdac B is identical to thailandepsin A independently isolated by Cheng from the same strain of Burkholderia thailandensis . Burkholdac B is the most potent among depsipeptide histone deacetylase inhibitors in growth inhibition of the MCF7 breast cancer cell line with an IC(50) of 60 pM. | 2011 | 22091906 |
| Alteration in cell surface properties of Burkholderia spp. during surfactant-aided biodegradation of petroleum hydrocarbons. | Chemical surfactants may impact microbial cell surface properties, i.e., cell surface hydrophobicity (CSH) and cell surface charge, and may thus affect the uptake of components from non-aqueous phase liquids (NAPLs). This work explored the impact of Triton X-100, Igepal CA 630, and Tween 80 (at twice the critical micelle concentration, CMC) on the cell surface characteristics of Burkholderia cultures, Burkholderia cepacia (ES1, aliphatic degrader) and Burkholderia multivorans (NG1, aromatic degr ... | 2011 | 22089390 |
| characterization and inference of gene gain/loss along burkholderia evolutionary history. | a comparative analysis of 60 complete burkholderia genomes was conducted to obtain insight in the evolutionary history behind the diversity and pathogenicity at species level. a concatenated multiprotein phyletic pattern and a dataset with burkholderia clusters of orthologous genes (bucogs) were constructed. the extent of horizontal gene transfer (hgt) was assessed using a markov based probabilistic method. a reconstruction of the gene gains and losses history shows that more than half of the bu ... | 2011 | 22084562 |
| burkholderia symbiotica sp. nov., isolated from root nodules of mimosa spp. native to north east brazil. | four strains, jpy345t, jpy347, jpy366 and jpy581 were isolated from nitrogen-fixing nodules on the roots of two mimosa spp. (m. cordistipula, m. misera) native to north east brazil, and their taxonomic positions were investigated by a polyphasic approach. all four strains grew at 15-43 °c (optimum, 35 °c), at ph 4.0-7.0 (optimum, ph 5.0) and with 0-2 % nacl (optimum, 0 %). on the basis of 16s rrna gene sequence analysis, a representative strain (jpy345t) showed 97.3 % sequence similarity to t ... | 2011 | 22081715 |
| production and characteristics of the whole-cell lipase from organic solvent tolerant burkholderia sp. zyb002. | the thermostable and organic solvent tolerant whole-cell lipase (wcl) was produced by burkholderia sp. zyb002 with broad spectrum organic solvent tolerance. the production medium of the wcl was primarily optimized, which resulted in the maximum activity of 22.8 u/ml and the 5.1-fold increase of the wcl yield. the optimized culture medium was as follows (% w/v or v/v): soybean meal 2, soybean oil 0.5, manganese sulfate 0.1, k(2)hpo(4) 0.1, olive oil 0.5, initial ph 6.0, inoculum density 2, liquid ... | 2011 | 22081330 |
| Musculoskeletal melioidosis. | Melioidosis is an infectious disease caused by Burkholderia pseudomallei, mostly affecting patients in Southeast Asia and northern Australia. The disease has been increasingly recognized around the world due to the increased levels of travel and population movement. Clinical manifestations of melioidosis range from fulminant septicemic illness to an indolent local infection. The disease often involves multiple organs, including the lung, spleen, liver, and other visceral organs. Musculoskeletal ... | 2011 | 22081283 |
| parallel bacterial evolution within multiple patients identifies candidate pathogenicity genes. | bacterial pathogens evolve during the infection of their human host(1-8), but separating adaptive and neutral mutations remains challenging(9-11). here we identify bacterial genes under adaptive evolution by tracking recurrent patterns of mutations in the same pathogenic strain during the infection of multiple individuals. we conducted a retrospective study of a burkholderia dolosa outbreak among subjects with cystic fibrosis, sequencing the genomes of 112 isolates collected from 14 individuals ... | 2011 | 22081229 |
| photodynamic antimicrobial chemotherapy (pact) in combination with antibiotics for treatment of burkholderia cepacia complex infection. | this study aimed to determine if photodynamic antimicrobial chemotherapy (pact) was effective in the treatment of burkholderia cepacia complex infection and whether a synergistic effect was evident if pact was used in combination with antibiotics. the susceptibility of both planktonic and biofilm cultures of b. cepacia complex strains to methylene blue (mb) and meso-tetra(n-methyl-4-pyridyl)porphine tetra-tosylate (tmp)-mediated pact was determined alone and in combination with antibiotics used ... | 2012 | 22079165 |
| A Burkholderia pseudomallei toxin inhibits helicase activity of translation factor eIF4A. | The structure of BPSL1549, a protein of unknown function from Burkholderia pseudomallei, reveals a similarity to Escherichia coli cytotoxic necrotizing factor 1. We found that BPSL1549 acted as a potent cytotoxin against eukaryotic cells and was lethal when administered to mice. Expression levels of bpsl1549 correlate with conditions expected to promote or suppress pathogenicity. BPSL1549 promotes deamidation of glutamine-339 of the translation initiation factor eIF4A, abolishing its helicase ac ... | 2011 | 22076380 |
| growth and cesium uptake responses of phytolacca americana linn. and amaranthus cruentus l. grown on cesium contaminated soil to elevated co2 or inoculation with a plant growth promoting rhizobacterium burkholderia sp. d54, or in combination. | growth and cesium uptake responses of plants to elevated co(2) and microbial inoculation, alone or in combination, can be explored for clean-up of contaminated soils, and this induced phytoextraction may be better than the natural process. the present study used open-top chambers to investigate combined effects of burkholderia sp. d54 inoculation and elevated co(2) (860 μl l(-1)) on growth and cs uptake by phytolacca americana and amaranthus cruentus grown on soil spiked with various levels of c ... | 2011 | 22074893 |
| Proteomic analysis of colony morphology variants of Burkholderia pseudomallei defines a role for the arginine deiminase system in bacterial survival. | Colony morphology variation of Burkholderia pseudomallei is a notable feature of a proportion of primary clinical cultures from patients with melioidosis. Here, we examined the hypothesis that colony morphology switching results in phenotypic changes associated with enhanced survival under adverse conditions. We generated isogenic colony morphology types II and III from B. pseudomallei strain 153 type I, and compared their protein expression profiles using 2D gel electrophoresis. Numerous protei ... | 2012 | 22062159 |
| extractive fermentation for improved production and recovery of lipase derived from burkholderia cepacia using a thermoseparating polymer in aqueous two-phase systems. | an extractive fermentation technique was developed using a thermoseparating reagent to form a two-phase system for simultaneous cell cultivation and downstream processing of extracellular burkholderia cepacia lipase. a 10% (w/w) solution of ethylene oxide-propylene oxide (eopo) with a molecular mass of 3900g/mol and ph 8.5, a 200rpm speed, and 30°c were selected as the optimal conditions for lipase production (55u/ml). repetitive batch fermentation was performed by continuous replacement of the ... | 2011 | 22061444 |
| burkholderia gladioli - a predictor of poor outcome in cystic fibrosis patients who receive lung transplants? a case of locally invasive rhinosinusitis and persistent bacteremia in a 36-year-old lung transplant recipient with cystic fibrosis. | there have been very few reports describing postlung transplant outcomes in patients' infected⁄colonized with burkholderia gladioli pretransplant. a case involving a lung transplant recipient with cystic fibrosis who ultimately died as a result of severe rhinosinusitis due to b gladioli infection in the context of postlung transplant immunosuppression is reported. | 2011 | 22059186 |
| Tsunami lung. | We encountered three cases of lung disorders caused by drowning in the recent large tsunami that struck following the Great East Japan Earthquake. All three were females, and two of them were old elderly. All segments of both lungs were involved in all the three patients, necessitating ICU admission and endotracheal intubation and mechanical ventilation. All three died within 3 weeks. In at least two cases, misswallowing of oil was suspected from the features noted at the time of the detection. ... | 2011 | 22057370 |
| O-Acetyl location on cepacian, the principal exopolysaccharide of Burkholderia cepacia complex bacteria. | Cepacian is an exopolysaccharide produced by the majority of the isolates belonging to the Burkholderia cepacia complex bacteria, a group of 17 species, some of which infect cystic fibrosis patients, sometime with fatal outcome. The repeating unit of cepacian consists of a backbone having a trisaccharidic repeating unit with three side chains, as reported in the formula below. The exopolysaccharide is also acetylated, carrying from one to three acetyl esters per repeating unit, depending on the ... | 2011 | 22055818 |
| Expression of Aeromonas caviae polyhydroxyalkanoate synthase gene in Burkholderia sp. USM (JCM15050) enables the biosynthesis of SCL-MCL PHA from palm oil products. | Aims: Burkholderia sp. USM (JCM15050) isolated from oil-polluted wastewater is capable of utilizing palm oil products and glycerol to synthesize poly(3-hydroxybutyrate) [P(3HB)]. To confer the ability to produce polymer containing 3-hydroxyhexanoate (3HHx), plasmid (pBBREE32d13) harbouring the polyhydroxyalkanoate (PHA) synthase gene of Aeromonas caviae (phaC(Ac) ) was transformed into this strain. Methods and Results: The resulting transformant incorporated approximately 1 ± 0·3 mol% of 3HHx ... | 2012 | 22054430 |
| [pneumonia-causing bacterial pathogens in intensive care patients]. | this prospective study aimed at determining etiologic agents causing nosocomial pneumonia in a precisely defined group of patients and resistance of bacterial pathogens to antimicrobial drugs. | 2011 | 22052100 |
| Profiling of Burkholderia cepacia secretome at mid-logarithmic and early-stationary phases of growth. | Burkholderia cepacia is a Gram-negative pathogen that causes serious respiratory infections in immunocompromised patients and individuals with cystic fibrosis. This bacterium is known to release extracellular proteins that may be involved in virulence. | 2011 | 22046299 |
| Diversity of 16S rRNA genes from bacteria of sugarcane rhizosphere soil. | Sugarcane is an important agricultural product of Brazil, with a total production of more than 500 million tons. Knowledge of the bacterial community associated with agricultural crops and the soil status is a decisive step towards understanding how microorganisms influence crop productivity. However, most studies aim to isolate endophytic or rhizosphere bacteria associated with the plant by culture-dependent approaches. Culture-independent approaches allow a more comprehensive view of entire ba ... | 2011 | 22042267 |
| determination of the crystal structure and active residues of fabv, the enoyl-acp reductase from xanthomonas oryzae. | enoyl-acp reductase (enr) catalyses the last reduction reaction in the fatty acid elongation cycle in bacteria and is a good antimicrobial target candidate. fabv is the most recently discovered class of enr, but we lack information about the atomic structure and the key residues involved in reductase activity except for the known conserved tyrosine and lysine residues in the y-x(8)-k active site motif. | 2011 | 22039545 |
| the phylogenetic distribution and ecological role of carbon monoxide oxidation in the genus burkholderia. | burkholderia is a physiologically and ecologically diverse genus that occurs commonly in assemblages of soil and rhizosphere bacteria. although burkholderia is known for its heterotrophic versatility, we demonstrate that 14 distinct environmental isolates oxidized carbon monoxide (co) and possessed the gene encoding the catalytic subunit of form i co dehydrogenase (coxl). dna from a burkholderia isolate obtained from a passalid beetle also contained coxl as do the genomic sequences of species h1 ... | 2011 | 22029898 |
| Molecular investigations of a locally acquired case of melioidosis in Southern AZ, USA. | Melioidosis is caused by Burkholderia pseudomallei, a Gram-negative bacillus, primarily found in soils in Southeast Asia and northern Australia. A recent case of melioidosis in non-endemic Arizona was determined to be the result of locally acquired infection, as the patient had no travel history to endemic regions and no previous history of disease. Diagnosis of the case was confirmed through multiple microbiologic and molecular techniques. To enhance the epidemiological analysis, we conducted s ... | 2011 | 22028940 |
| myd88-dependent recruitment of monocytes and dendritic cells required for protection from pulmonary burkholderia mallei infection. | the gram-negative bacterium burkholderia mallei causes rapidly fatal illness in equines and humans when contracted by inhalation and also has the potential to be used as a bioweapon. however, little is known regarding the early innate immune responses and signaling mechanisms required to generate protection from pneumonic b. mallei infection. we showed previously that monocyte chemoattractant protein 1 (mcp-1) was a critical chemokine required for protection from pneumonic b. mallei infection. w ... | 2012 | 22025508 |
| the type vi secretion system of burkholderia cenocepacia affects multiple rho family gtpases disrupting the actin cytoskeleton and the assembly of nadph oxidase complex in macrophages. | burkholderia cenocepacia is a gram-negative opportunistic pathogen of patients with cystic fibrosis and chronic granulomatous disease. the bacterium survives intracellularly in macrophages within a membrane-bound vacuole (bccv) that precludes the fusion with lysosomes. the underlying cellular mechanisms and bacterial molecules mediating these phenotypes are unknown. here, we show that intracellular b. cenocepacia expressing a type vi secretion system (t6ss) affects the activation of the rac1 and ... | 2011 | 22023353 |
| Burkholderia cenocepacia disrupts host cell actin cytoskeleton by inactivating Rac and Cdc42. | Burkholderia cenocepacia, a member of the Burkholderia cepacia complex, is an opportunistic pathogen that causes devastating infections in patients with cystic fibrosis. The ability of B. cenocepacia to survive within host cells could contribute significantly to its virulence in immunocompromised patients. In this study, we explored the mechanisms that enable B. cenocepacia to survive inside macrophages. We found that B. cenocepacia disrupts the actin cytoskeleton of infected macrophages, drast ... | 2011 | 22023324 |
| Two Norwegian patients with melioidosis presenting with bacteraemia and splenic and prostatic abscesses. | Infections caused by Burkholderia pseudomallei are rare in nonendemic areas, such as Scandinavia. We report the first two cases of melioidosis in Norway presenting with bacteraemia and splenic and prostatic abscesses, respectively. | 2011 | 22017720 |
| screening of freeze-dried protective agents for the formulation of biocontrol strains, bacillus cereus ar156, burkholderia vietnamiensis b418 and pantoea agglomerans 2re40. | the effects of different freeze-drying protective agents on the viabilities of biocontrol strains bacillus cereus ar156, burkholderia vietnamiensis b418 and pantoea agglomerans 2re40 were investigated. | 2012 | 22017666 |
| application of a burkholderia cepacia lipase-immobilized silica monolith to batch and continuous biodiesel production with a stoichiometric mixture of methanol and crude jatropha oil. | abstract: | 2011 | 22013896 |
| characterization of the volatile profile of antarctic bacteria by using solid-phase microextraction-gas chromatography-mass spectrometry. | bacteria belonging to the burkholderia cepacia complex (bcc) are significant pathogens in cystic fibrosis (cf) patients and are resistant to a plethora of antibiotics. in this context, microorganisms from antarctica are interesting because they produce antimicrobial compounds inhibiting the growth of other bacteria. this is particularly true for bacteria isolated from antarctic sponges. the aim of this work was to characterize a set of antarctic bacteria for their ability to produce new natural ... | 2011 | 22012672 |
| Use of 3-hydroxy fatty acid concentrations in a murine air pouch infection model as a surrogate marker for LPS activity: a feasibility study using environmental Burkholderia cenocepacia isolates. | Using a murine hypodermic air pouch infection model designed to mimic the release of bacterial products at physiological levels, 3-hydroxy fatty acid (3-OH FA) and endotoxin unit levels from Burkholderia cenocepacia isolates were assessed. The B. cenocepacia environmental isolates (n=35) survived in the hypodermic air pouch but did not invade across the peritoneal epithelial layer during a 72-h infection. For all 35 strains, when the molar ratio of C(14:0) 3-OH FA to C(16:0) 3-OH FA in the air p ... | 2011 | 22008505 |
| strategies for intracellular survival of burkholderia pseudomallei. | burkholderia pseudomallei is the causative agent of melioidosis, a disease with high mortality that is prevalent in tropical regions of the world. a key component of the pathogenesis of melioidosis is the ability of b. pseudomallei to enter, survive, and replicate within mammalian host cells. for non-phagocytic cells, bacterial adhesins have been identified both on the bacterial surface and associated with type 4 pili. cell invasion involves components of one or more of the three type 3 secretio ... | 2011 | 22007185 |