Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| an immunohistochemical method for the diagnosis of melioidosis. | in order to assess the usefulness of immunohistochemistry in the diagnosis of melioidosis, an infection by burkholderia pseudomallei, polyclonal antibodies were applied to tissues from known cases of melioidosis and to other infected tissues. formalin-fixed, paraffin-embedded tissues were stained by a modified immunoperoxidase technique. in autopsy tissues with inflammatory lesions of melioidosis, the cytoplasm of phagocytes and intact bacilli, both intra- and extracellular, were stained very st ... | 1996 | 8743829 |
| the environmental risk factors associated with medical and dental equipment in the transmission of burkholderia (pseudomonas) cepacia in cystic fibrosis patients. | transmission of burkholderia (pseudomonas) cepacia by close contact with colonized patients is well documented, and minimizing social contact between cystic fibrosis (cf) patients by segregation and cohorting of b. cepacia colonized patients has achieved some success in controlling the nosocomial and community spread of this organism. however, direct and indirect environmental transmission still occurs. there is evidence for transmission of b. cepacia to cf patients via pulmonary test equipment, ... | 1996 | 8744509 |
| survival of burkholderia cepacia on environmental surfaces. | burkholderia (pseudomonas) cepacia is an important pathogen amongst persons with cystic fibrosis (cf), and evidence suggests that transmission of strains within cf clinics contributes to pulmonary colonization of some patients. in order to optimize preventive strategies, the survival of b. cepacia on various environmental surfaces, including cotton cloth, stainless steel, latex and polyvinylchloride (pvc) tubing, was investigated. for surface inoculation, bacteria were suspended in phosphate buf ... | 1996 | 8744511 |
| enzyme-linked immunosorbent assay (elisa) using a glycolipid antigen for the serodiagnosis of melioidosis. | the serodiagnosis of melioidosis is commonly performed with tests using protein or polysaccharide as antigen. however, due to the low sensitivity, specificity and difficulty in the preparation of the antigens, more simple, precise and reproducible diagnostic tests were required. a purified glycolipid antigen (gl) which is a specific lipid component of burkholderia pseudomallei has been used in an elisa. with this antigen, specific immunoglobulin g (igg) was detected in 49 out of 50 melioidosis s ... | 1995 | 8745012 |
| prognostic significance of quantitative bacteremia in septicemic melioidosis. | pour-plate blood cultures were performed for 418 adult patients with suspected septicemic melioidosis in order to determine the relationship between quantitative bacterial counts in blood and mortality. of 108 patients whose hemocultures yielded burkholderia pseudomallei, 53% had < 10 cfu/ml and 24% had > 100 cfu/ml. high blood bacterial counts were more common than reported previously with regard to other gram-negative septicemias and were significantly associated with the development of hypote ... | 1995 | 8749644 |
| [structure of the o-specific polysaccharide chain from burkholderia (pseudomonas) solanacerum icmp 750, 8093, 8115, 7864, and 7945 lipopolysaccharides]. | lipopolysaccharides (lps) have been isolated from the cells of phytopathogenic bacteria burkholderia (pseudomonas) solanacearum strains (representatives of i, ii and iv biovars) using mild acid hydrolysis followed by gel permeation chromatography of carbohydrate residues on sephadex g-50 to yield five o-specific polysaccharides (o-ps). the o-ps structures were established by 13c-nmr spectroscopy. it has been found that o-ps have a linear or branched structure. the former represents a tetrasaccha ... | 1996 | 8754268 |
| [antigenic activity of burkholderia solanacearum lipopolysaccharides]. | the interaction of the antiserum against burkholderia solanacearum icmp 8110 cells with lipopolysaccharides from 19 strains of b. solanacearum differing in o-specific polysaccharide structure has been studied using elisa. no correlation was found between the o-specific polysaccharide structure and serological activity of the lipopolysaccharide. most of the lipopolysaccharides isolated from b. solanacearum strains (except for 4157, 767 and 7942) displayed cross-reactivity with the tested antiseru ... | 1996 | 8754270 |
| pseudomonas (burkholderia) cepacia in children with cystic fibrosis: epidemiological investigation by analysis or restriction fragment length polymorphism. | since 1987, pseudomonas cepacia has been isolated with an increasing frequency in the expectorants of children with cystic fibrosis followed at the hôpital d'enfants armand trousseau (paris, france). colonization by p. cepacia may be responsible for serious secondary infections and rapid deterioration in respiratory function in these patients. among the 130 children attending our centre, 14 (8 girls and 6 boys) aged 3 to 18, exhibited chronic colonization. 132 isolates, originating from sputum o ... | 1996 | 8758491 |
| 2,4-dinitrotoluene dioxygenase from burkholderia sp. strain dnt: similarity to naphthalene dioxygenase. | 2,4-dinitrotoluene (dnt) dioxygenase from burkholderia sp. strain dnt catalyzes the initial oxidation of dnt to form 4-methyl-5-nitrocatechol (mnc) and nitrite. the displacement of the aromatic nitro group by dioxygenases has only recently been described, and nothing is known about the evolutionary origin of the enzyme systems that catalyze these reactions. we have shown previously that the gene encoding dnt dioxygenase is localized on a degradative plasmid within a 6.8-kb nsii dna fragment (w.- ... | 1996 | 8759857 |
| molecular cloning and overexpression of a glutathione transferase gene from proteus mirabilis. | the structural gene of the proteus mirabilis glutathione transferase gstb1-1 (gstb) has been isolated from genomic dna. a nucleotide sequence determination of gstb predicted a translational product of 203 amino acid residues, perfectly matching the sequence of the previously purified protein [mignogna, allocati, aceto, piccolomini, di ilio, barra and martini (1993) eur. j. biochem. 211, 421-425]. the p. mirabilis gst sequence revealed 56% identity with the escherichia coli gst at dna level and 5 ... | 1996 | 8761466 |
| douglas-fir root-associated microorganisms with inhibitory activity towards fungal plant pathogens and human bacterial pathogens. | a microbial culture collection composed of 1820 bacterial strains, including 298 actinomycete strains, was established from the roots of douglas-fir (pseudotsuga menziesii (mirb.) franco) seedlings harvested from conifer nurseries and forest sites. two hundred and thirty-four strains inhibited the growth of fusarium, cylindrocarpon, and (or) pythium spp. in in vitro assays. a significantly greater proportion of bacterial strains from actinomycete genera exhibited antifungal properties compared w ... | 1996 | 8764683 |
| a phosphonate monoester hydrolase from burkholderia caryophilli pg2982 is useful as a conditional lethal gene in plants. | a bacterial phosphonate monoester hydrolase was evaluated in plants as a conditional lethal gene useful for cell ablation and negative selection. glyphosate is a potent herbicide whereas its phosphonate monoester derivative, glyceryl glyphosate, is approximately 50-fold less active. a phosphonate monoesterase gene (peha) encoding an enzyme that hydrolyzes phosphonate esters including glyceryl glyphosate to glyphosate and glycerol was cloned from the glyphosate metabolizing bacterium, burkholderi ... | 1996 | 8771792 |
| characterization of a chromosomally encoded 2,4-dichlorophenoxyacetic acid/alpha-ketoglutarate dioxygenase from burkholderia sp. strain rasc. | the findings of previous studies indicate that the genes required for metabolism of the pesticide 2,4-dichlorophenoxyacetic acid (2,4-d) are typically encoded on broad-host-range plasmids. however, characterization of plasmid-cured strains of burkholderia sp. strain rasc, as well as mutants obtained by transposon mutagenesis, suggested that the 2,4-d catabolic genes were located on the chromosome of this strain. mutants of burkholderia strain rasc unable to degrade 2,4-d (2,4-d- strains) were ob ... | 1996 | 8779585 |
| capture of a catabolic plasmid that encodes only 2,4-dichlorophenoxyacetic acid:alpha-ketoglutaric acid dioxygenase (tfda) by genetic complementation. | the modular pathway for the metabolism of 2,4-dichlorophenoxyacetic acid (2,4-d) encoded on plasmid pjp4 of alcaligenes eutrophus jmp134 appears to be an example in which two genes, tfda and tfdb, have been recruited during the evolution of a catabolic pathway. the products of these genes act to convert 2,4-d to a chloro-substituted catechol that can be further metabolized by enzymes of a modified ortho-cleavage pathway encoded by tfdcdef. given that modified ortho-cleavage pathways are comparat ... | 1996 | 8779586 |
| no cross-reactivity with filamentous hemagglutinin of bordetella pertussis in sera from patients with nontypable haemophilus influenzae pneumonia. | 1996 | 8783367 | |
| nosocomial outbreak of burkholderia pickettii infection due to a manufactured intravenous product used in three hospitals. | forty-six cases of nosocomial infection caused by burkholderia pickettii were reported between june and november 1993 in three metropolitan hospitals in madrid. a case-control study of the outbreak was conducted to identify its cause. seventy-four percent of the patients were males; the mean age +/- sd of the patients was 54 +/- 20 years. sixty-five percent of the patients presented with some gastrointestinal disorder, and 80% had a peripheral catheter; 98% were treated with intravenous fluids, ... | 1996 | 8783718 |
| identification of is1356, a new insertion sequence, and its association with is402 in epidemic strains of burkholderia cepacia infecting cystic fibrosis patients. | burkholderia cepacia is now recognized as an important opportunistic pathogen in cystic fibrosis (cf) and other compromised patients. epidemicity among cf patients has been attributed to at least one particularly infectious strain (strain et12), and both genetic evidence and anecdotal evidence suggest that this strain, currently endemic in ontario, and those causing an epidemic in the united kingdom, are indeed the same. our study was conducted to determine whether there was any association betw ... | 1996 | 8784555 |
| differentiation of thermolysins and serralysins by monoclonal antibodies. | two monoclonal antibodies (mabs) to a 36-kda extracellular metalloprotease (pscp) from burkholderia (pseudomonas) cepacia were found to react with thermolysin, pseudomonas aeruginosa elastase, alkaline protease (apr) and lasa, serratia marcescens protease (smp), aeromonas hydrophila protease (ahp), and both the lethal factor (lf) and protective antigen (pa) of bacillus anthracis on immunoblots. the mabs were capable of neutralising the proteolytic activity of thermolysin, p. aeruginosa elastase ... | 1996 | 8810950 |
| accuracy of four commercial systems for identification of burkholderia cepacia and other gram-negative nonfermenting bacilli recovered from patients with cystic fibrosis. | burkholderia cepacia has recently been recognized as an important pathogen in chronic lung disease in patients with cystic fibrosis (cf). because of the social, psychological, and medical implications of the isolation of b. cepacia from cf patients, accurate identification of this organism is essential. we compared the accuracies of four commercial systems developed for the identification of nonfermenting, gram-negative bacilli with that of conventional biochemical testing for 150 nonfermenters ... | 1996 | 8815102 |
| rapid antigen detection assay for identification of burkholderia (pseudomonas) pseudomallei infection. | a simple antigen detection test was developed for rapid diagnosis of melioidosis caused by burkholderia (pseudomonas) pseudomallei infection. the method was based on the use of a specific monoclonal antibody in a sandwich enzyme-linked immunosorbent assay to capture antigen in clinical specimens culture positive for b. pseudomallei. the results showed the sensitivity and specificity of the test to be 75 and 98%, respectively. | 1996 | 8815121 |
| treatment of cystic fibrosis in adults. | more patients with cystic fibrosis are surviving into adulthood. primary care physicians need a basic understanding of adult cystic fibrosis and the evaluation of patients with acute decompensation. respiratory decompensation is usually the result of infective agents, including pseudomonas aeruginosa, stentrophomonas maltophilia and burkholderia cepacia, and requires treatment with intravenous antibiotics. if symptoms worsen, the possibility of another complication, such as pneumothorax, hemopty ... | 1996 | 8816573 |
| field inversion gel electrophoresis on pseudomonas cepacia strains isolated from cystic fibrosis patients. | genome fingerprinting by field inversion gel electrophoresis (fige) was utilized to typify 129 isolates of pseudomonas cepacia (pc) from 59 patients with cystic fibrosis (cf) and from environmental cultures in the cf ward. the aim of this study was to assess whether a segregation policy avoided colonization of cf patients by nosocomial strains and contamination of the environment by colonized individuals, whether or not an 'epidemic strain' was present in the ward and whether cross-colonization ... | 1996 | 8817193 |
| [thermostable pseudomonas pseudomallei hemolysin]. | the chemical nature of thermostable p.pseudomallei hemolysin has been determined. this substance is glycolipid, synthesized by bacteria at the stationary phase of growth under the conditions of the prevalence of the source of carbon over the source of nitrogen in the nutrient medium. the hemolytic activity of thermostable hemolysin has a delayed character and is more pronounced in p.pseudomallei r-forms. | 1996 | 8820670 |
| [the immune response to pseudomonas pseudomallei in vitro]. | the results of studies made with the aim of selecting conditions for inducing immune response to p.pseudomallei are presented. the study revealed that the dose of the antigen, the amount of macrophages and the development of the process in stages are the decisive factors for inducing immune response. the proliferation of macrophages till their predominance in the culture was found to depend on the dose of the antigen. some mitogens increased the antigen-induced proliferation of macrophages. the ... | 1996 | 8820682 |
| empyema and bloodstream infection caused by burkholderia gladioli in a patient with cystic fibrosis after lung transplantation. | 1996 | 8823866 | |
| identification, characterization, and cloning of a phosphonate monoester hydrolase from burkholderia caryophilli pg2982. | the glyphosate-degrading bacterium, burkholderia caryophilli pg2982, was observed to utilize glyceryl glyphosate as a sole phosphorus source. the hydrolysis of glyceryl glyphosate to glyphosate by a phosphonate ester hydrolase (peh) was identified as the first metabolic step in the mineralization pathway. this observation provides the first biological role for a phosphonate ester hydrolase activity. purified peh enzyme hydrolyzed several phosphonate esters including p-nitrophenyl phenylphosphona ... | 1996 | 8824203 |
| ptim3, a plasmid delivery vector for a transposon-based inducible marker gene system in gram-negative bacteria. | ptim3 is a suicide plasmid vector for the delivery of a transposition defective derivative of tn5, expressing inducible mercury resistance (hgr) and catechol 2,3-dioxygenase (c23o) activity, to a range of gram-negative microorganisms. ptim3 was constructed by a four-stage process from pnmm1, a derivative of psup5011 containing a modified tn5 where antibiotic-resistance determinants have been replaced by the mer operon of tn501 and tdnc (encoding a c23o). in ptim3, tdnc is fused to merd, to bring ... | 1995 | 8825369 |
| burkholderia (pseudomonas) cepacia epidemiology in a cystic fibrosis population: a genome finger-printing study. | in patients with cystic fibrosis, infection with pseudomonas cepacia is associated with poor outcomes. however, the epidemiology of burkholderia cepacia is still unclear. the aim of this study was to investigate the epidemiology of burkholderia (pseudomonas) cepacia colonization among cystic fibrosis patients attending the verona cf centre, a large specialized unit to which patients from different parts of italy are admitted. we used a genome finger-printing system to analyse the nucleotidic str ... | 1996 | 8827098 |
| 4-methylphthalate catabolism in burkholderia (pseudomonas) cepacia pc701: a gene encoding a phthalate-specific permease forms part of a novel gene cluster. | we have determined the entire nucleotide sequence of a 4.4 kbp fragment of pmop, a plasmid involved in 4-methylphthalate catabolism in burkholderia cepacia (formerly pseudomonas cepacia) pc701. two complete orfs were identified and termed mopa and mopb. mopb encodes a 4-methylphthalate permease which is a member of a superfamily of symport proteins found in both prokaryotes and eukaryotes. functionality was assigned to mopb by detailed analysis of the predicted amino acid sequence, resulting in ... | 1996 | 8828207 |
| purification and sequence analysis of 4-methyl-5-nitrocatechol oxygenase from burkholderia sp. strain dnt. | 4-methyl-5-nitrocatechol (mnc) is an intermediate in the degradation of 2,4-dinitrotoluene by burkholderia sp. strain dnt. in the presence of nadph and oxygen, mnc monooxygenase catalyzes the removal of the nitro group from mnc to form 2-hydroxy-5-methylquinone. the gene (dntb) encoding mnc monooxygenase has been previously cloned and characterized. in order to examine the properties of mnc monooxygenase and to compare it with other enzymes, we sequenced the gene encoding the mnc monooxygenase a ... | 1996 | 8830701 |
| nucleotide sequence analysis of a gene from burkholderia (pseudomonas) cepacia encoding an outer membrane lipoprotein involved in multiple antibiotic resistance. | antibiotic-resistant burkholderia (pseudomonas) cepacia is an important etiologic agent of nosocomial and cystic fibrosis infections. the primary resistance mechanism which has been reported is decreased outer membrane permeability. we previously reported the cloning and characterization of a chloramphenicol resistance determinant from an isolate of b. cepacia from a patient with cystic fibrosis that resulted in decreased drug accumulation. in the present studies we subcloned and sequenced the r ... | 1996 | 8834871 |
| laboratory investigation of ecological factors influencing the environmental presence of burkholderia pseudomallei. | factors like temperature, ph value, water content in soil, and ultraviolet rays that might have influence on the survival of environmental burkholderia pseudomallei strains were evaluated. data showed that the optimal temperature and ph value for b. pseudomallei were 24 c to 32 c and 5 to 8, respectively. water content in soil of less than 10% brought about the death of the bacteria within 70 days, while water content of more than 40% maintained bacteria life for 726 days. the bacteria were easi ... | 1996 | 8839431 |
| environmental sampling to detect burkholderia cepacia in a cystic fibrosis outpatient clinic. | 1996 | 8839652 | |
| melioidosis: two indigenous cases in taiwan. | we report the first two indigenously acquired cases of melioidosis in taiwan, diagnosed by positive culture and biochemically identified using the id 32 gn system (biomerieux vitek inc, hazelwood, mo, usa). the first patient was a 75-year-old chinese woman who had not travelled abroad since her arrival from mainland china (san-tung province) 47 years ago. she presented with spontaneous bacterial peritonitis and hepatitis c-related liver cirrhosis with septic shock. burkholderia pseudomallei (for ... | 1996 | 8840761 |
| microbial pathogenesis in cystic fibrosis: mucoid pseudomonas aeruginosa and burkholderia cepacia. | respiratory infections with pseudomonas aeruginosa and burkholderia cepacia play a major role in the pathogenesis of cystic fibrosis (cf). this review summarizes the latest advances in understanding host-pathogen interactions in cf with an emphasis on the role and control of conversion to mucoidy in p. aeruginosa, a phenomenon epitomizing the adaptation of this opportunistic pathogen to the chronic chourse of infection in cf, and on the innate resistance to antibiotics of b. cepacia, person-to-p ... | 1996 | 8840786 |
| chronic granulomatous disease presenting as severe sepsis due to burkholderia gladioli. | 1996 | 8842296 | |
| investigation of nosocomial respiratory infection due to pseudomonas cepacia by arbitrarily primed polymerase chain reaction. | we used dna fingerprinting by the arbitrarily primed polymerase chain reaction (ap-pcr) technique for an epidemiologic investigation of pseudomonas cepacia nosocomial isolates obtained from patients attending our hospital. this approach was compared with conventional phenotypic typing and pulsed-field gel electrophoresis (pfge). the patterns of gel electrophoresis of the products of ap-pcr differed significantly according to differences in the concentration of mg2+ and in ph. ap-pcr and pfge was ... | 1995 | 8849650 |
| [utility of pyrrolidonyl-arylamidase detection for typing enterobacteriaceae and non-fermenting gram-negative bacteria]. | detection of pyrrolidonyl-aryl-amidase activity (pyr) is an important tool to identify gram-positive cocci, such as staphylococci, enterococci, streptococci, and other related genera. however, only few studies evaluating its usefulness with gram-negative rods have been published. thus, a prospective study including 542 and 215 unique clinical isolates of enterobacteriaceae and non-fermentative gram-negative rods, respectively, was undertaken. strains were identified by conventional methods. pyr ... | 1995 | 8850133 |
| susceptibilities of non-pseudomonas aeruginosa gram-negative nonfermentative rods to ciprofloxacin, ofloxacin, levofloxacin, d-ofloxacin, sparfloxacin, ceftazidime, piperacillin, piperacillin-tazobactam, trimethoprim-sulfamethoxazole, and imipenem. | agar dilution mics of 10 agents against 410 non-pseudomonas aeruginosa gram-negative nonfermentative rods were determined. mics at which 50 and 90% of the isolates were inhibited, respectively, were as follows (in micrograms per milliliter): sparfloxacin, 0.5 and 8.0; levofloxacin, 1.0 and 8.0; ciprofloxacin, 2.0 and 32.0; ofloxacin, 2.0 and 32.0; d-ofloxacin, 32.0 and > 64.0; ceftazidime, 8.0 and 64.0; piperacillin with or without tazobactam, 16.0 and > 64.0; trimethoprim-sulfamethoxazole, 0.5 ... | 1996 | 8851609 |
| molecular analysis of burkholderia (pseudomonas) cepacia: differentiation between relapse and reinfection in a case of recurrent bacteremia. | 1996 | 8852993 | |
| burkholderia (pseudomonas) cepacia and cystic fibrosis: the epidemiology in belgium. | burkholderia cepacia has become an increasingly recognized pathogen among cystic fibrosis (cf) patients and its potential role in declining pulmonary function or unexpected fatal outcome has caused widespread concern. direct person-to-person transmission has been documented and a segregation policy of cf patients colonized with b.cepacia from non-colonized cf patients is widely adopted. since this policy has a dramatic impact on social behaviour of cf patients it is imperative that clinical labo ... | 1996 | 8858887 |
| immunobiological diagnosis of tropical ocular diseases: toxocara, pythium insidiosum, pseudomonas (burkholderia) pseudomallei, mycobacterium chelonei and toxoplasma gondii. | of the sera obtained from 18 patients with ocular diseases comprising ocular larva migrans (olm), toxoplasma gondii, mycobacterium chelonei, pythium insidiosum bacteria and tumour, 3 sera were positive for toxocara antibody at the titre over 1/1600 elisa. all 3 of these sera came from males with unilateral grayish fundi. we demonstrated the value of the direct immunofluorescent assay (difa) on vitreous specimens from 7 cases of toxoplasma retinitis. one vitreous specimen under electronmicroscopi ... | 1996 | 8880376 |
| antimicrobial activity of superoxidized water. | we tested the antimicrobial activity of superoxidized water against methicillin-sensitive staphylococcus aureus, methicillin-resistant staphylococcus aureus, staphylococcus epidermidis, serratia marcescens, escherichia coli, pseudomonas aeruginosa and burkholderia cepacia. the number of bacteria was reduced below detection limit following incubation in superoxidized water for 10 s. the bactericidal activity of superoxidized water was similar to that of 80% ethanol, but superior to that of 0.1% c ... | 1996 | 8880549 |
| bacterial contamination of commercially available ethacridine lactate (acrinol) products. | bacterial contamination of commercially available ethacridine lactate (acrinol) solutions and cotton gauze soaked in ethacridine lactate solution was investigated. of 56 samples from ethacridine lactate solutions (eight products, seven manufacturers), seven samples (12.5%) of two products (two manufacturers) were contaminated with 10(1)-10(4) colony forming units (cfu)/ml of burkholderia pickettii. of 67 samples obtained from gauze soaked in ethacridine lactate solution (seven products, seven ma ... | 1996 | 8880550 |
| an outbreak of pseudobacteraemia caused by burkholderia pickettii: the critical role of an epidemiological link. | a protracted outbreak of pseudobacteraemia caused by burkholderia pickettii is reported. initial investigation did not reveal the source of the outbreak. subsequently, careful examination of patient records provided an insight to the probable source, which was further confirmed by a case control study. it was found that 48% of cases had either hepatobiliary diseases or haematological malignancies, whereas only 15% of the controls had similar problems. furthermore, a coagulation study was perform ... | 1996 | 8880551 |
| distribution and transmission of pseudomonas aeruginosa and burkholderia cepacia in a hospital ward. | genotyping and antibiotic susceptibility testing were used to analyze pseudomonas aeruginosa and burkholderia cepacia strains from sink drain from 14 pediatric patients with cystic fibrosis (cf) and from hospital personnel as part of a 4 week prospective study of strain transmission in a pediatric ward. a total of 87.5% of all washbasin drains were contaminated with p. aeruginosa [10(2) to 10(5) colony forming units (cfu)/ml sink fluid], whereas b. cepacia was found only once in a sink drain. fr ... | 1996 | 8882212 |
| assay for bacteria in porous media by diffusion-weighted nmr. | in this work, an nmr technique capable of detecting bacterial cells and measuring the cell density in suspension and in porous media has been developed. it is based on the pulsed-field-gradient technique and relies on the fact that extracellular water diffuses freely while intracellular water is completely restricted by the relatively impermeable cell wall of the bacterium. at high wave vectors, the signal from extracellular water is completely suppressed while the signal from intracellular wate ... | 1996 | 8888588 |
| a genetic analysis system of burkholderia cepacia: construction of mobilizable transposons and a cloning vector. | a genetic analysis system of burkholderia cepacia (bc) was developed which included transposon mutagenesis and complementation of mutation with the cloned genes of interest. to deliver the transposon in this multidrug-resistant microorganism, two plasmids, pkn30 and pkn31, were constructed which contained tn5 derivatives, tn5-30tp and tn5-31tp, respectively, carrying kmr and tpr genes. the plasmids have the origin of cole1 replication and the mobilization gene of rp4. tn5-31tp was mobilized to b ... | 1996 | 8890733 |
| cascade regulation of the toluene-3-monooxygenase operon (tbua1ubva2c) of burkholderia pickettii pko1: role of the tbua1 promoter (ptbua1) in the expression of its cognate activator, tbut. | burkholderia pickettii pko1 metabolizes toluene and benzene via a chromosomally encoded toluene-3-monooxygenase pathway. expression of the toluene-3-monooxygenase operon (tbua1ubva2c) is activated by the regulator, tbut, in the presence of toluene. we have identified the tbut coding region downstream of the toluene-3-monooxygenase structural genes by nucleotide sequence analysis and have shown that although tbut is similar to xylr and dmpr, two members of the ntrc family of transcriptional activ ... | 1996 | 8892837 |
| purification of hydroxyquinol 1,2-dioxygenase and maleylacetate reductase: the lower pathway of 2,4,5-trichlorophenoxyacetic acid metabolism by burkholderia cepacia ac1100. | the enzyme hydroxyquinol 1,2-dioxygenase, which catalyzes ortho cleavage of hydroxyquinol (1,2,4-trihydroxybenzene) to produce maleylacetate, was purified from escherichia coli cells containing the tfth gene from burkholderia cepacia ac1100. reduction of the double bond in maleylacetate is catalyzed by the enzyme maleylacetate reductase, which was also purified from e. coli cells, these cells containing the tfte gene from b. cepacia ac1100. the two enzymes together catalyzed the conversion of hy ... | 1996 | 8900023 |
| genomic complexity and plasticity of burkholderia cepacia. | burkholderia cepacia has attracted attention because of its extraordinary degradative abilities and its potential as a pathogen for plants and for humans. this bacterium was formerly considered to belong to the genus pseudomonas in the gamma-subclass of the proteobacteria, but recently has been assigned to the beta-subclass is based on rrn gene sequence analyses and other key phenotypic characteristics. the b. cepacia genome is comprised of multiple chromosomes and is rich in insertion sequences ... | 1996 | 8900054 |
| identification and sequencing of a novel insertion sequence, is1471, in burkholderia cepacia strain 2a. | a novel insertion sequence (is), is1471, has been identified which is inserted into is element is1071 possessed by plasmid pijb1 in burkholderia cepacia strain 2a. nucleotide sequencing has revealed that is1471 is 1112 bp in length and is flanked by 22/21-bp imperfect inverted repeats with a 3-bp duplication of the target sequence is1471 contains a single open reading frame encoding a putative polypeptide of 345 amino acids with molecular mass of 39406 da. searches of dna and protein sequence da ... | 1996 | 8900064 |
| structure of the o-specific polysaccharide from burkholderia pickettii strain nctc 11149. | a polymeric fraction (the putative o antigen) has been isolated from the lipopolysaccharide of the type strain of burkholderia pickettii. the components of the polymer and their molar proportions were: l-rhamnose (3), 2-acetamido-2-deoxy-d-glucose (1), and o-acetyl (1). by means of nmr studies and chemical degradations, the basic repeating-unit of the polymer was identified as a linear tetrasaccharide of the structure shown. the o-acetyl group is probably located at position 2 of the 3-substitut ... | 1996 | 8901090 |
| microbial contamination of antiseptics and disinfectants. | there have been a number of reports on microbial contamination of antiseptics and disinfectants. at present, however, the necessity of measures to prevent contamination do not seem to be fully appreciated. we investigated microbial contamination of antiseptics and disinfectants that are used in our hospital. | 1996 | 8902114 |
| in vitro activity of sparfloxacin against selected enterobacteriaceae and pseudomonaceae including ps. pseudomallei (melioidosis). | 1995 | 8904106 | |
| is burkholderia (pseudomonas) cepacia disseminated from cystic fibrosis patients during physiotherapy? | burkholderia cepacia is well recognized as a potential respiratory pathogen in cystic fibrosis (cf) patients and there is increasing concern about nosocomial acquisition. environmental contamination with b. cepacia before, during and after physiotherapy was studied in eight adult cf patients. air was sampled using a surface air sampler, and horizontal surfaces and pillows were sampled with moistened swabs and contact plates, respectively. thirty-nine (40%) of 97 air samples were positive and cou ... | 1996 | 8904368 |
| bone graft contamination from a water de-ionizer during processing in a bone bank. | an organism resembling burkholderia (formerly pseudomonas) cepacia was isolated from cultures taken during processing of six bone grafts. the source of the contamination was traced to a water de-ionizer; the problem had begun when procedures had been changed, so that water from the de-ionizer had been used without sterilization. cultures of bone for grafting not only protect recipients from infection, but act also as useful controls for processes involved in bone banking. | 1996 | 8904374 |
| home-use nebulizers: a potential primary source of burkholderia cepacia and other colistin-resistant, gram-negative bacteria in patients with cystic fibrosis. | inhalation of aerosols contaminated with gram-negative bacteria generated from home-use nebulizers used by cystic fibrosis (cf) patients may be a primary route for bacterial colonization of the lung. burkholderia cepacia was isolated from 3 of [corrected] 35 home-use nebulizers, and stenotrophomonas maltophilia was isolated from 4 of 35 home-use nebulizers. sputum cultures for two patients whose nebulizers were contaminated with b. cepacia did not yield the organism. however, dna macrorestrictio ... | 1996 | 8904419 |
| detection of burkholderia pseudomallei dna in patients with septicemic melioidosis. | septicemic melioidosis is the most severe form of melioidosis, which is caused by burkholderia pseudomallei. it is endemic in southeast asia and is the leading cause of death from community-acquired septicemia in northeast thailand. a major factor that contributes to the high mortality is the delay in isolation and identification of the causative organism. more than half of the patients die within the first 2 days after hospital admission, before bacterial cultures become positive. the present s ... | 1996 | 8904424 |
| bronchiolitis obliterans organizing pneumonia (boop) in lung transplant recipients. | we reviewed all tissue specimens from 163 transplant patients (108 double lung transplant [dlt], 55 single lung transplant [slt]) between november 1983 and january 1994 for abnormalities indicating bronchiolitis obliterans organizing pneumonia (boop) and found 17 cases (14 dlt and 3 slt). of the three slts, boop was diagnosed by open lung biopsy (olb) in two and one was found at autopsy. of the 14 dlts, boop was diagnosed by transbronchial biopsy (tbb) specimens (9), olb specimens (2), autopsy ( ... | 1996 | 8915212 |
| identification and nucleotide sequence of rhizobium meliloti insertion sequence isrm6, a small transposable element that belongs to the is3 family. | the insertion sequence isrm6 is a small transposable element identified in rhizobium meliloti strain gr4 by sequence analysis. two copies of this is element were found in strain gr4, one of them is linked to the nfe genes located on plasmid prmegr4b. isrm6 seems to be widespread in r. meliloti. data suggest that isrm6 is active in transposition at an estimated frequency of 2 x 10(-5) per generation per cell in strain gr4. this 1269-bp element carries 27/26-bp terminal imperfect inverted repeats ... | 1996 | 8917074 |
| melioidosis presenting as epididymo-orchitis. | we report a rare case of suppurative epididymo-orchitis caused by pseudomonas pseudomallei in a 56-year-old male. this is a gram negative bacillus found mainly in tropical zones. diagnosis was reached by culture of the organisms after drainage of the scrotal abscess, and the patient was treated by a course of oral chloramphenicol 500 mg qid for 6 months. | 1995 | 8919168 |
| trichloroethylene degradation and mineralization by pseudomonads and methylosinus trichosporium ob3b. | to examine the trichloroethylene (c2hcl3)-degrading capability of five microorganisms, the maximum rate, extent, and degree of c2hcl3 mineralization were evaluated for pseudomonas cepacia g4, pseudomonas cepacia g4 pr1, pseudomonas mendocina kr1, pseudomonas putida f1, and methylosinus trichosporium ob3b using growth conditions commonly reported in the literature for expression of oxygenases responsible for c2hcl3 degradation. by varying the c2hcl3 concentration from 5 microm to 75 microm, vmax ... | 1996 | 8920197 |
| [simultaneous isolation of mrsa and pseudomonas aeruginosa using a novel selective and differential pmac agar]. | pmac agar, a novel, selective and differential medium has been developed and was subjected for evaluation of its selective and differential capability of methicillin resistant staphylococcus aureus (mrsa) and pseudomonas aeruginosa from other bacteria such as bacillus, micrococcus, gram-negative bacteria and drug resistant ones. growth of mrsa and p. aeruginosa on pmac agar was facilitated and their colonies were easily differentiated. colonies of mrsa after 24 approximately 48 h incubation at 3 ... | 1996 | 8921677 |
| lack of evidence of nosocomial cross-infection by burkholderia cepacia among danish cystic fibrosis patients. | burkholderia cepacia isolates from nine of the ten danish cystic fibrosis (cf) patients known between 1975 and the present day to carry this organism were investigated. eight distinct genotypes were found with polymerase chain reaction ribotyping and pulsed-field gel electrophoresis. the results indicate that there is little patient-to-patient cross-infection with burkholderia cepacia within the danish cf population, even though the majority of patients attend the same cf clinic on a regular bas ... | 1996 | 8922580 |
| transformation of burkholderia pseudomallei by electroporation. | conditions were defined for the successful transformation of the human pathogen burkholderia pseudomallei 4845 by electroporation, using the incq plasmid pkt230. we have obtained frequencies of up to 8 x 10(3) transformants per microgram plasmid dna with freshly prepared electrocompetent b. pseudomallei. the method also allows for easy and reproducible production of frozen cell suspensions which can produce efficiencies up to 2.5 x 10(2) transformants per microgram of plasmid dna. kanamycin had ... | 1996 | 8923967 |
| emerging & re-emerging bacterial pathogens in india. | in spite of major successes against infectious diseases in the 20th century, new infectious diseases have emerged and old ones re-emerged in recent decades in different parts of the world. a brief survey of emerging and re-emerging bacterial diseases of public health importance in india is presented in this paper. plague re-appeared in two outbreaks in maharashtra and gujarat in 1994, indicating a breakdown of the public health measures that had prevented its occurrence for several decades. lept ... | 1996 | 8926026 |
| melioidosis in india: the tip of the iceberg? | diagnosis of melioidosis by the isolation of burkholderia pseudomallei from one or more body fluid/tissue specimens of 6 indian subjects, 5 of whom had not travelled outside india, is reported. the places of residence of these 6 and one patient previously reported, namely tripura (2), kerala (2), orissa (1), tamil nadu (1) and maharashtra (1) are therefore potentially endemic for melioidosis. b.pseudomallei closely resembles common contaminant pseudomonas sp. and are easily mis-identified in mic ... | 1996 | 8926030 |
| invasion of respiratory epithelial cells by burkholderia (pseudomonas) cepacia. | pulmonary infections caused by burkholderia (pseudomonas) cepacia are an important cause of morbidity and mortality in cystic fibrosis (cf) patients. several features suggestive of cellular invasion and intracellular sequestration of b. cepacia in cf are persistence of infection in the face of antibiotic therapy to which the organism demonstrates in vitro susceptibility and a propensity to cause bacteremic infections in patients with cf. epithelial cell invasion was demonstrated in vitro in a549 ... | 1996 | 8926068 |
| the dsbb gene product is required for protease production by burkholderia cepacia. | burkholderia cepacia kf1, isolated from a pneumonia patient, produces a 37-kda extracellular metalloprotease. a protease-deficient and lipase-proficient mutant, kft1007, was complemented by a clone having an open reading frame coding for a 170-amino-acid polypeptide which showed significant homology to escherichia coli dsbb. kft1007, a presumed dsbb mutant, also failed to show motility, and both protease secretion and motility were restored by the introduction of the cloned dsbb gene of b. cepac ... | 1996 | 8926116 |
| exploration of the relationship between tetrachlorohydroquinone dehalogenase and the glutathione s-transferase superfamily. | tetrachlorohydroquinone dehalogenase is found in sphingomonas chlorophenolica, a soil bacterium that degrades pentachlorophenol, a widely used wood preservative. this enzyme converts tetrachlorohydroquinone (tchq) to trichlorohydroquinone (trichq) and trichq to dichlorohydroquinone (dchq) (xun et al. (1992) j. bacteriol. 174, 8003-8007). the reducing equivalents for each step are provided by two molecules of glutathione (xun et al. (1992) biochem. biophys. res. commun. 182, 361-366). in addition ... | 1996 | 8931562 |
| nosocomial burkholderia cepacia outbreaks and pseudo-outbreaks. | 1996 | 8934237 | |
| three-year outbreak of pseudobacteremia with burkholderia cepacia traced to a contaminated blood gas analyzer. | between november 1990 and june 1993, burkholderia cepacia was isolated from the blood cultures of 13 neonates born at the ottawa general hospital. eight of the 13 neonates appeared symptomatic, and only 4 were treated with appropriate antimicrobial therapy, but all improved clinically. in august 1993, the blood gas analyzer in the neonatal intensive-care unit was found to be contaminated heavily with b cepacia. eight available patient isolates were identical to the isolates recovered from the bl ... | 1996 | 8934241 |
| an outbreak of burkholderia cepacia lower respiratory tract infection associated with contaminated albuterol nebulization solution. | an outbreak of burkholderia cepacia lower respiratory tract colonization and infection occurred in the adult intensive-care units in various geographic locations throughout our hospital. forty-four patients became colonized or infected over an 11-month period, whereas b cepacia had been isolated from only 13 patients in the preceding 48 months. environmental cultures revealed the source to be extrinsically contaminated albuterol nebulization solution. polymerase chain reaction-ribotyping confirm ... | 1996 | 8934242 |
| biological activities of lipopolysaccharide of burkholderia (pseudomonas) pseudomallei. | endotoxic activities of lipopolysaccharide (lps) isolated from burkholderia (pseudomonas) pseudomallei, a causative agent of melioidosis, were investigated. compared to an enterobacterial lps (sae-lps), b. pseudomallei lps (bp-lps) exhibited weaker pyrogenic activity in rabbits, lethal toxicity in galactosamine-sensitized mice and murine macrophage activation, i.e. production of tumor necrosis factor, interleukin-6 and nitric oxide. bp-lps, on the other hand, exhibited stronger mitogenic activit ... | 1996 | 8935661 |
| epidemiology of burkholderia cepacia infection in patients with cystic fibrosis: analysis by randomly amplified polymorphic dna fingerprinting. | we fingerprinted a collection of 627 burkholderia cepacia isolates from 255 patients with cystic fibrosis (cf) and 43 patients without cf and from the environment, by a pcr-based randomly amplified polymorphic dna (rapd) method with primers selected for their ability to produce discriminatory polymorphisms. the rapd typing method was found to be reproducible and discriminatory, more sensitive than pcr ribotyping, and able to group epidemiologically related b. cepacia strains previously typed by ... | 1996 | 8940422 |
| burkholderia pseudomallei activates complement and is ingested but not killed by polymorphonuclear leukocytes. | the mechanism by which burkholderia pseudomallei is resistant to lysis by human serum is unknown but may include interference with complement activation, effective opsonization, or complement-mediated lysis. we investigated the interaction of b. pseudomallei with complement in the presence and absence of specific antibody to determine potential mechanisms of serum resistance. we demonstrated rapid activation and consumption of complement by b. pseudomallei which, in the absence of specific antib ... | 1996 | 8945532 |
| epidemiology of pulmonary colonization with burkholderia cepacia in cystic fibrosis patients. the french observatoire burkholderia cepacia study group. | 1996 | 8950569 | |
| measurement of three-bond coupling constants in the osmoregulated periplasmic glucan of burkholderia solanacearum. | the cyclic osmoregulated periplasmic glucan produced by burkholderia solanacearum contains 13 glucose units, all beta-(1-2) linked except for one alpha-(1-6) linkage. we report here the measurement of the 3j(c1-h2') and 3j(h1-c2') coupling constants, characterizing the glycosidic linkages, through the use of a 13c/12c double half-filtered noesy experiments. the values obtained give information about the (phi, psi) angles of the different linkages. the results presented from an important step tow ... | 1996 | 8953219 |
| biodegradation of 2,4,5-trichlorophenoxyacetic acid by burkholderia cepacia strain ac1100: evolutionary insight. | many microorganisms in nature have evolved new genes which encode catabolic enzymes specific for chlorinated aromatic substrates, allowing them to utilize these compounds as sole sources of carbon and energy. an understanding of the evolutionary mechanisms involved in the acquisition of such genes may facilitate the development of microorganisms with enhanced capabilities of degrading highly chlorinated recalcitrant compounds. a number of studies have been based on microorganisms isolated from t ... | 1996 | 8955624 |
| evaluation of contamination of the dentist and dental surgery environment with burkholderia (pseudomonas) cepacia during treatment of children with cystic fibrosis. | pulmonary colonization with burkholderia (pseudomonas) cepacia in cystic fibrosis (cf) patients has been associated with high morbidity and mortality. the aim of this study was to investigate whether the dentist and the dental surgery environment become contaminated during treatment of cf patients colonized with b. cepacia. extensive environmental sampling was carried out during aerosol-generating treatment sessions on four cf patients whose sputum was persistently colonized with b. cepacia. sam ... | 1995 | 8957838 |
| burkholderia cepacia: medical, taxonomic and ecological issues. | the increasing challenge posed by multiresistant saprophytes in medical microbiology is strikingly demonstrated by the emergence of burkholderia (formerly pseudomonas) cepacia as an opportunist pathogen in immunocompromised patients, particularly individuals with chronic granulomatous disease and cystic fibrosis (cf). best known previously as a phytopathogen and the cause of soft rot of onions, b. cepacia presents three major problems for the cf community: innate multiresistance to antimicrobial ... | 1996 | 8958242 |
| biochemical characteristics of clinical and environmental isolates of burkholderia pseudomallei. | the biochemical characteristics of 213 isolates of burkholderia pseudomallei from patients with melioidosis and 140 isolates from the soil in central and northeastern thailand were compared. whereas the biochemical profiles of all the clinical isolates were similar, all soil isolates from the central area and 25% of isolates from northeastern thailand comprised a different phenotype. this was characterised by the ability to assimilate l-arabinose (100%), adonitol (100%), 5-keto-gluconate (90%) a ... | 1996 | 8958243 |
| rp4::mu3a-mediated in vivo cloning and transfer of a chlorobiphenyl catabolic pathway. | chromosomal dna fragments encoding the ability to utilize biphenyl as sole carbon source (bph+) were mobilized by means of plasmid rp4::mu3a from strain jb1 (tentatively identified as burkholderia sp.) to alcaligenes eutrophus ch34 at a frequency of 10(-3) per transferred plasmid. the mobilized dna integrated into the recipient chromosome or was recovered as catabolic prime plasmids. three bph+ prime plasmids were transferred from a. eutrophus to escherichia coli and back to a. eutrophus without ... | 1996 | 8969525 |
| comparison of factors influencing trichloroethylene degradation by toluene-oxidizing bacteria. | the degradation of trichloroethylene (tce) by toluene-oxidizing bacteria has been extensively studied, and yet the influence of environmental conditions and physiological characteristics of individual strains has received little attention. to consider these effects, the levels of tce degradation by strains distinguishable on the basis of toluene and nitrate metabolism were compared under aerobic or hypoxic conditions in the presence and absence of nitrate and an exogenous electron donor, lactate ... | 1996 | 8975612 |
| succession and convergence of biofilm communities in fixed-film reactors treating aromatic hydrocarbons in groundwater. | community composition, succession, and performance were compared in three fluidized bed reactors (fbr) operated to test preemptive colonization and the influence of toluene compared with a mixture of benzene, toluene, and p-xylene (btx) as feeds. one reactor was inoculated with toluene-degrading strains pseudomonas putida paw1, burkholderia cepacia g4, and b. pickettii pko1. paw1 outcompeted the other two strains. when groundwater strains were allowed to challenge the steady-state biofilm develo ... | 1997 | 8979355 |
| characterization of the agrobacterium vitis peha gene and comparison of the encoded polygalacturonase with the homologous enzymes from erwinia carotovora and ralstonia solanacearum. | dna sequencing of the agrobacterium vitis peha gene revealed a predicted protein with an m(r) of 58,000 and significant similarity to the polygalacturonases of two other plant pathogens, erwinia carotovora and ralstonia (= pseudomonas or burkholderia) solanacearum. sequencing of the n terminus of the peha protein demonstrated cleavage of a 34-amino-acid signal peptide from pre-peha. mature peha accumulated primarily in the periplasm of a. vitis and peha+ escherichia coli cells during exponential ... | 1997 | 8979363 |
| disseminated melioidosis. | 1996 | 8979590 | |
| production of specific monoclonal antibodies to burkholderia pseudomallei and their diagnostic application. | hybridomas secreting monoclonal antibodies (mabs) specific to burkholderia pseudomallei were produced by immunizing balb/cj mice with crude culture filtrate of b. pseudomallei. two monoclonal antibodies were found to be highly specific to b.pseudomallei as tested by indirect enzyme-linked immunosorbent assay and immunoblotting against a panel of crude whole cell extracts from b. pseudomallei, b. cepacia, pseudomonas aeruginosa, p.putida, and escherichia coli. one of the specific mabs, clone sp-m ... | 1996 | 8980799 |
| bacterial colonization as a potential source of nosocomial respiratory infections in two types of spirometer. | the potential risk of spirometers in the transmission of respiratory infections has not been yet established. we performed a prospective cross-sectional study to determine the rate of colonization of a water-sealed spirometer and a pneumotachograph, and the potential risk of cross-transmission of microorganisms to patients using each of these devices. fifty four patients (aged 51 +/- 18 (mean +/- sd) yrs) were included in the study. all of them had undergone forced spirometry with bronchodilator ... | 1996 | 8980977 |
| trichloroethylene oxidation by purified toluene 2-monooxygenase: products, kinetics, and turnover-dependent inactivation. | trichloroethylene is oxidized by several types of nonspecific bacterial oxygenases. toluene 2-monooxygenase from burkholderia cepacia g4 is implicated in trichloroethylene oxidation and is uniquely suggested to be resistant to turnover-dependent inactivation in vivo. in this work, the oxidation of trichloroethylene was studied with purified toluene 2-monooxygenase. all three purified toluene 2-monooxygenase protein components and nadh were required to reconstitute full trichloroethylene oxidatio ... | 1997 | 8981984 |
| 2-naphthoate catabolic pathway in burkholderia strain jt 1500. | burkholderia strain (jt 1500), able to use 2-naphthoate as the sole source of carbon, was isolated from soil. on the basis of growth characteristics, oxygen uptake experiments, enzyme assays, and detection of intermediates, a degradation pathway of 2-naphthoate is proposed. the features of this pathway are convergent with those for phenanthrene. we propose a pathway for the conversion of 2-naphthoate to 1 mol (each) of pyruvate, succinate, and acetyl coenzyme a and 2 mol of co2. during growth in ... | 1997 | 8981987 |
| pigb determines a diffusible factor needed for extracellular polysaccharide slime and xanthomonadin production in xanthomonas campestris pv. campestris. | seven xanthomonadin transcriptional units (piga through pigg) were identified by transposon saturation mutagenesis within an 18.6-kbp portion of the previously identified 25.4-kbp pig region from xanthomonas campestris pv. campestris (strain b-24). since marker exchange mutant strains with insertions in one 3.7-kbp portion of pig could not be obtained, mutations in this region may be lethal to the bacterium. complementation analyses with different insertion mutations further defined and confirme ... | 1997 | 8990296 |
| systematic study of the genus vogesella gen. nov. and its type species, vogesella indigofera comb. nov. | a blue-pigmented colony that had a metallic copper-colored sheen was isolated in 1973 from a standard spread plate count preparation of oxidation pond sediment. over the next 11 years, an additional 12 strains of blue-pigmented bacteria were isolated from freshwater samples and compared to several reference strains of bacteria. morphological and biochemical tests revealed that these 13 isolates were very similar to [pseudomonas] indigofera atcc 19706t (t = type strain) and atcc 14036. a numerica ... | 1997 | 8995797 |
| molecular epidemiology of burkholderia cepacia, stenotrophomonas maltophilia, and alcaligenes xylosoxidans in a cystic fibrosis center. | burkholderia cepacia, stenotrophomonas maltophilia, and alcaligenes xylosoxidans have been isolated with increasing frequency from the sputum of patients with cystic fibrosis in a pediatric hospital. in 1994-95, 27 of 120 patients were persistently colonized, 17 with burkholderia cepacia, eight with alcaligenes xylosoxidans, and five with stenotrophomonas maltophilia. genotyping of 220 clinical isolates revealed that most of the burkholderia cepacia strains were clonally related, suggesting eith ... | 1996 | 8997562 |
| structure of the o-specific polysaccharide from burkholderia vietnamiensis strain lmg 6998. | the putative o-specific polymer containing d-mannose and l-rhamnose was isolated from the lipopolysaccharide obtained from cells walls of burkholderia vietnamiensis strain lmg 6998. nmr and degradative studies showed that the polymer has a linear trisaccharide repeating-unit of the structure shown. the same polymer carrying an o-acetyl group at position 3 of the 4-substituted mannose residue has previously been found as the o antigen in the related species burkholderia cepacia serogroup j. [form ... | 1996 | 9002192 |
| identification of neutralizing epitopes on pseudomonas aeruginosa elastase and effects of cross-reactions on other thermolysin-like proteases. | monoclonal antibodies (mabs) to a burkholderia (pseudomonas) cepacia 36-kda protease (pscp) which neutralize pscp and pseudomonas aeruginosa elastase but not p. aeruginosa alkaline protease have been isolated (c. kooi et al., infect. immun. 62:2811-2817, 1994). these mabs, designated 36-6-6 and 36-6-8, react with n-chlorosuccinimide cleavage products of p. aeruginosa elastase, consistent with the recognition of a 13.9-kda fragment which contains the active site. overlapping 9-mer peptides that s ... | 1997 | 9009299 |
| induction of biologically active interleukin-8 from lung epithelial cells by burkholderia (pseudomonas) cepacia products. | the frequency of isolation of burkholderia cepacia from the sputum of cystic fibrosis (cf) patients is increasing. using the human a549 lung epithelial cell line, we have investigated the ability of b. cepacia exoproducts to stimulate interleukin-8 (il-8) release. cell-free supernatants from a panel of cf clinical, non-cf clinical, and nonclinical b. cepacia isolates were found to stimulate il-8 release, with levels ranging from 11.8 +/- 2.8 to 80.0 +/- 3.5 ng/ml. a similar pattern was seen at t ... | 1997 | 9009321 |
| structural studies of the acidic exopolysaccharide produced by a mucoid strain of burkholderia cepacia, isolated from cystic fibrosis. | the acidic exopolysaccharide produced by a mucoid strain of burkholderia cepacia isolated from a cystic fibrosis patient, was purified by cetyltrimethylammonium bromide precipitation and/or anion-exchange chromatography. based on the sugar composition and permethylation analyses, supported by glc-ms and nmr spectroscopy analyses, the repeating-unit of the polysaccharide was established as -->3)-beta-d-glcp-(1-->3)-[4,6-o-(1-carboxyethylidene)]-alpha-d-gal p-(1-->. | 1996 | 9011377 |
| polymerase chain reaction for the detection of pseudomonas aeruginosa, stenotrophomonas maltophilia and burkholderia cepacia in sputum of patients with cystic fibrosis. | occurrence of pseudomonas aeruginosa, stenotrophomonas (xanthomonas) maltophilia and burkholderia (pseudomonas) cepacia in sputum of cystic fibrosis (cf) patients was demonstrated with a simple and rapid polymerase chain reaction (pcr) technique. the pcr was performed with a set of three primer pairs based on 16s rrna sequences after sputum preparation with dithiothreitol and naoh lysis. all three pathogens could be individually detected by the use of this technique. to prevent carry-over contam ... | 1996 | 9025076 |