Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| current studies on the pathogenesis of melioidosis. | burkholderia pseudomallei is a major cause of bacterial septicemias in many parts of the world, particularly thailand; the known geographic range of the organism appears to be enlarging as awareness of the organism and the disease it causes--melioidosis--increases. b. pseudomallei is intrinsically resistant to most antibiotics, and our knowledge of b. pseudomallei pathogenesis is lacking. thus, the long-term objective of our research is to define at a molecular level the pathogenesis by combinin ... | 1999 | 10594980 |
| [the epidemiology of burkholderia cepacia in cystic fibrosis patients: the initial results of a study including the genotypic typing of the strains isolated]. | 1999 | 10596443 | |
| burkholderia cepacia is resistant to the antimicrobial activity of airway epithelial cells. | there has been much interest recently in the antimicrobial properties of cationic peptides called beta-defensins from epithelial cells. human beta-defensin (hbd)-1 and -2 have been particularly implicated in cystic fibrosis (cf) patients, where their inhibition by high salt concentrations may explain in part the susceptibility of the cf lung to bacterial infection. in this work, we have employed a simple co-culture system using the 16-hbe human bronchial epithelial cell line to assess growth inh ... | 1999 | 10598883 |
| invasion and intracellular survival of burkholderia cepacia. | burkholderia cepacia has emerged as an important pulmonary pathogen in immunocompromised patients and in patients with cystic fibrosis (cf). little is known about the virulence factors and pathogenesis of b. cepacia, although the persistent and sometimes invasive infections caused by b. cepacia suggest that the organism possesses mechanisms for both cellular invasion and evasion of the host immune response. in this study, cultured human cells were used to analyze the invasion and intracellular s ... | 2000 | 10603364 |
| stable marker on flagellin gene sequences related to arabinose non-assimilating pathogenic burkholderia pseudomallei. | using pcr-based isolation and sequence analysis of the flagellin gene from two distinct biotypes of burkholderia pseudomallei, a 15-bp deletion was found within the variable domain of the gene in isolates capable of assimilating arabinose (ara+). this finding led to the development of a pcr-based method in order to differentiate and identify pathogenic b. pseudomallei for epidemiological study. a pair of specific primers was designed covering the 15-bp deletion region at the variable domain. pcr ... | 1999 | 10609608 |
| continuous degradation of mixtures of 4-nitrobenzoate and 4-aminobenzoate by immobilized cells of burkholderia cepacia strain pb4. | although isolated on 4-aminobenzoate, burkholderia cepacia strain pb4 is also able to grow on 4-nitrobenzoate. degradation of an equimolar mixture of the nitroaromatic compound 4-nitrobenzoate and its corresponding aminoaromatic derivative 4-aminobenzoate by this strain was investigated. batch experiments showed that, irrespective of preculturing conditions, both compounds were degraded simultaneously. the mixture-degrading ability of b. cepacia strain pb4 was subsequently tested in continuous p ... | 1999 | 10610092 |
| species versus biotype status. | 1999 | 10610379 | |
| an exotic pulmonary infection in thailand: melioidosis. | melioidosis is an infectious disease from burkholderia pseudomallei and is confined in specific geographic areas such as southeast asia. its highest prevalence in thailand is in the north-eastern part. most infected patients had worked paddy fields or had underlying diseases such as diabetes mellitus. melioidosis can manifest clinically, with either disseminated or localized features. in the disseminated form patients developed an acute and progressive course with septicaemia. in contrast, patie ... | 1999 | 10612579 |
| pulmonary function, serum markers of inflammation, and igg antibodies to core lipopolysaccharide of burkholderia cepacia in adults with cystic fibrosis, following colonization with burkholderia cepacia. | eight patients with cystic fibrosis [cf] colonized with pseudomonas aeruginosa (p. aeruginosa) had serial lung function, peripheral blood inflammatory markers, and serum igg antibodies to burkholderia cepacia (b. cepacia) lipopolysaccharide measured in the months preceding and following colonisation with b. cepacia. one patient experienced a fall in fev(1) from 33% to 19% of predicted values, coinciding with the first sputum isolation of b. cepacia, and he died 12 weeks later. he had a rise in i ... | 2000 | 10613780 |
| identification of a new class of 5'-adenylylsulfate (aps) reductases from sulfate-assimilating bacteria. | a gene was cloned from burkholderia cepacia dbo1 that is homologous with escherichia coli cysh encoding 3'-phosphoadenylylsulfate (paps) reductase. the b. cepacia gene is the most recent addition to a growing list of cysh homologs from a diverse group of sulfate-assimilating bacteria whose products show greater homology to plant 5'-adenylylsulfate (aps) reductase than they do to e. coli cysh. the evidence reported here shows that the cysh from one of the species, pseudomonas aeruginosa, encodes ... | 2000 | 10613872 |
| pathogenicity of microbes associated with cystic fibrosis. | cystic fibrosis patients are exceptionally prone to colonisation by a narrow spectrum of pathogenic bacteria. since pulmonary infection presently, and for the foreseeable future, plays such a major role in cf lung disease, we review the microbes that are classically associated with cf and the virulence, inflammatory potential and resistance mechanisms which contribute to the reduction in life expectancy for colonised cf patients. | 1999 | 10617932 |
| species-specific pcr as a tool for the identification of burkholderia gladioli. | burkholderia gladioli colonizes the respiratory tracts of patients with cystic fibrosis and chronic granulomatous disease. however, due to the high degree of phenotypic similarity between this species and closely related species in the burkholderia cepacia complex, accurate identification is difficult. incorrect identification of these species may have serious repercussions for the management of patients with cystic fibrosis. to develop an accurate procedure for the identification of b. gladioli ... | 2000 | 10618102 |
| natural transformation of acinetobacter sp. strain bd413 with cell lysates of acinetobacter sp., pseudomonas fluorescens, and burkholderia cepacia in soil microcosms. | to elucidate the biological significance of dead bacterial cells in soil to the intra- and interspecies transfer of gene fragments by natural transformation, we have exposed the kanamycin-sensitive recipient acinetobacter sp. strain bd413(pfg4) to lysates of the kanamycin-resistant donor bacteria acinetobacter spp., pseudomonas fluorescens, and burkholderia cepacia. detection of gene transfer was facilitated by the recombinational repair of a partially (317 bp) deleted kanamycin resistance gene ... | 2000 | 10618225 |
| detection and characterization of plasmid pjp4 transfer to indigenous soil bacteria. | prior to gene transfer experiments performed with nonsterile soil, plasmid pjp4 was introduced into a donor microorganism, escherichia coli atcc 15224, by plate mating with ralstonia eutropha jmp134. genes on this plasmid encode mercury resistance and partial 2, 4-dichlorophenoxyacetic acid (2,4-d) degradation. the e. coli donor lacks the chromosomal genes necessary for mineralization of 2,4-d, and this fact allows presumptive transconjugants obtained in gene transfer studies to be selected by p ... | 2000 | 10618238 |
| detection of francisella tularensis in biological specimens using a capture enzyme-linked immunosorbent assay, an immunochromatographic handheld assay, and a pcr. | the early detection of francisella tularensis, the causative agent of tularemia, is important for adequate treatment by antibiotics and the outcome of the disease. here we describe a new capture enzyme-linked immunosorbent assay (celisa) based on monoclonal antibodies specific for lipopolysaccharide (lps) of francisella tularensis subsp. holarctica and francisella tularensis subsp. tularensis. no cross-reactivity with francisella tularensis subsp. novicida, francisella philomiragia, and a panel ... | 2000 | 10618283 |
| burkholderia pseudomallei infections. | 2000 | 10619778 | |
| intracellular survival of burkholderia cepacia complex isolates in the presence of macrophage cell activation. | strains of the burkholderia cepacia complex have emerged as a serious threat to patients with cystic fibrosis due to their ability to infect the lung and cause, in some patients, a necrotizing pneumonia that is often lethal. it has recently been shown that several strains of the b. cepacia complex can escape intracellular killing by free-living amoebae following phagocytosis. in this work, the ability of two b. cepacia complex strains to resist killing by macrophages was explored. using fluoresc ... | 1999 | 10627044 |
| [septicemic melioidosis in southern taiwan: a case report]. | the patient was a 56 year-old man, a resident of hen-tsueng township in ping-tung county. he worked as a ranger at ken-ting farm in southern taiwan, and had been to thailand for sight-seeing 5 years ago. he came to our outpatient department about one month prior to hospitalization for intermittent fever of one week duration. at that time, complete blood count was within normal limits and a chest roentgenogram was unremarkable. he was given erythromycin without showing any clinical improvement. t ... | 1998 | 10627169 |
| serum bactericidal and inhibitory titres in the management of melioidosis. | a retrospective evaluation of the relationship between serum bactericidal and inhibitory titres and treatment outcome in 195 adult thai patients with severe melioidosis was conducted. drug regimens included ceftazidime (52% of patients), co-amoxiclav (24%), imipenem (11%) or the conventional four-drug combination (11%). pre- and 1 h post-dose serum samples were collected after 48-72 h of therapy, and serum inhibitory and bactericidal titrations determined. median post-dose titres were: bacterici ... | 2000 | 10629024 |
| in vitro analysis of roles of a disulfide bridge and a calcium binding site in activation of pseudomonas sp. strain kwi-56 lipase. | the expression of lipase from pseudomonas sp. strain kwi-56 (recently reclassified as burkholderia cepacia) had been found to be dependent on an activator gene (act) downstream of its structural gene (lip). in this work, the mature lipase was synthesized in an enzymatically active form with a cell-free escherichia coli s30 coupled transcription-translation system by expressing a recombinant lipase gene (rlip) encoding the mature lipase in the presence of its purified activator or by coexpression ... | 2000 | 10629173 |
| assessment of the extent and activity of melioidosis with technetium labelled leukocyte scanning. | 1999 | 10630658 | |
| purification and properties of extracellular carboxyl proteases of acid-tolerant bacteria, isolated from compost. | four strains of acid-tolerant and protein-using bacteria were isolated from compost. two of them, gram-negative strains mb8 and mb11, were identified as a new genus close to stenotrophomonas species mb8 and burkholderia species mb11, respectively. both bacteria produced extracellular carboxyl proteases (cp) in acid-casein-starch medium. the enzymes, termed cp mb8 and cp mb11, purified through ion exchange and gel filtration chromatographies had molecular weights of 61,000 (cp mb8) and 36,000 (cp ... | 1999 | 10635557 |
| a scalable protocol for the isolation of large-sized genomic dna within an hour from several bacteria. | 2000 | 10640359 | |
| effect of inoculation of a tol plasmid containing mycorrhizosphere bacterium on development of scots pine seedlings, their mycorrhizosphere and the microbial flora in m-toluate-amended soil. | the purpose of this study was to evaluate the influence of introduced bacteria containing a contaminant degrading plasmid on the growth and survival of pine seedlings and mycorrhizosphere microbial flora in contaminated soil. the pseudomonas fluorescens strain os81, originally isolated from fungal hyphae in contaminated soil, was supplied with the tol plasmid pww0::km (to generate os81(pww0::km)) and inoculated in humus-soil microcosms with and without pine seedlings mycorrhized with suillus bov ... | 2000 | 10640666 |
| skull osteomyelitis and multiple brain abscesses. | 2000 | 10644399 | |
| a comparison of lysis centrifugation, pour plate, and conventional blood culture methods in the diagnosis of septicaemic melioidosis. | to determine whether quantitative blood culture methods could improve the diagnosis of septicaemic melioidosis. | 1999 | 10645233 |
| initial in vitro characterisation of phosphonopyruvate hydrolase, a novel phosphate starvation-independent, carbon-phosphorus bond cleavage enzyme in burkholderia cepacia pal6. | a novel, inducible carbon-phosphorus bond cleavage enzyme, phosphonopyruvate hydrolase, was detected in cell-free extracts of burkholderia cepacia pal6, an environmental isolate capable of mineralising l-phosphonoalanine as carbon, nitrogen and phosphorus source. the activity was induced only in the presence of phosphonoalanine, did not require phosphate starvation for induction and was uniquely specific for phosphonopyruvate, producing equimolar quantities of pyruvate and inorganic phosphate. t ... | 2000 | 10648102 |
| acetyl substitution of the o-specific caryan from the lipopolysaccharide of pseudomonas (burkholderia) caryophylli leads to a block pattern. | an exceptionally large repeating unit has been proposed for one of the two o-specific polysaccharides of the lipopolysaccharide from pseudomonas (burkholderia) caryophylli, which is a homopolymer of the sugar caryose. this proposal is based on the unusual acetylation pattern of the carbohydrate (shown in the picture), as determined from the presence of eight independent spin systems observed by nmr spectroscopy. a=acetylated, d=non-acetylated caryose monomer. | 2000 | 10649359 |
| quantitative structure-toxicity relationships for chlorophenols to bioluminescent lux-marked bacteria using atom-based semi-empirical molecular-orbital descriptors. | literature data on the toxicity of chlorophenols for three luminescent bacteria (vibrio fischeri, and the lux-marked pseudomonas fluorescens 10586s pucd607 and burkholderia spp. rasc c2 (tn4431)) have been analyzed in relation to a set of computed molecular physico-chemical properties. the quantitative structure-toxicity relationships of the compounds in each species showed marked differences when based upon semi-empirical molecular-orbital molecular and atom based properties. for mono-, di- and ... | 1999 | 10649840 |
| identification of new transposable genetic elements in burkholderia pseudomallei using subtractive hybridisation. | a subtraction library of burkholderia pseudomallei was constructed by subtractive hybridisation of b. pseudomallei genomic dna with burkholderia thailandensis genomic dna. two clones were found to have significant sequence similarity to insertion sequences which have previously not been found in b. pseudomallei (designated isa and isb); and two clones showed sequence similarity to different regions of burkholderia cepacia is407 that has recently been detected in b. pseudomallei. the former, thou ... | 2000 | 10650205 |
| burkholderia pseudomallei traced to water treatment plant in australia. | burkholderia pseudomallei was isolated from environmental specimens 1 year after an outbreak of acute melioidosis in a remote coastal community in northwestern australia. b. pseudomallei was isolated from a water storage tank and from spray formed in a ph-raising aerator unit. pulsed-field gel electrophoresis confirmed the aerator and storage tank isolates were identical to the outbreak strain, wko97. | 2000 | 10653571 |
| specific and rapid detection by fluorescent in situ hybridization of bacteria in clinical samples obtained from cystic fibrosis patients. | we report on the rapid and specific detection of bacteria commonly isolated from clinical specimens from cystic fibrosis (cf) patients by fluorescent in situ hybridization (fish). on the basis of comparative sequence analysis, we designed oligonucleotide probes complementary to species-specific 16s rrna regions of these microorganisms and demonstrated the specificities of the probes by hybridization of different remotely related as well as closely related reference strains. furthermore, in a pil ... | 2000 | 10655391 |
| presence of type iii secretion genes in burkholderia pseudomallei correlates with ara(-) phenotypes. | dot blot hybridization and pcr amplification of 14 ara(+) and 8 ara(-) burkholderia pseudomallei strains showed that type iii secretion (tts) genes were present in all the ara(-) strains but absent from all but one of the ara(+) strains. the link between tts genes and an ara(-) phenotype suggests a role for tts in virulence. | 2000 | 10655407 |
| diagnostically and experimentally useful panel of strains from the burkholderia cepacia complex. | two new species, burkholderia multivorans and burkholderia vietnamiensis, and three genomovars (genomovars i, iii, and iv) currently constitute the burkholderia cepacia complex. a panel of 30 well-characterized strains representative of each genomovar and new species was assembled to assist with identification, epidemiological analysis, and virulence studies on this important group of opportunistic pathogens. | 2000 | 10655415 |
| propionic acid metabolism and poly-3-hydroxybutyrate-co-3-hydroxyvalerate (p3hb-co-3hv) production by burkholderia sp. | mutants of burkholderia sp. that are unable to grow on propionic acid (prp) but still accumulate p3hb-co-3hv from carbohydrate and propionic acid were studied. in shaken flask tests, yields of 3hv from propionic acid (y(3hv/prop)) increased from 0.10 g g(-1) in the wild type to c.a. 0.35 g g(-1) in mutants affected in alpha-oxidation pathway or to 0.80 g g(-1) in mutants not affected in that pathway. in bioreactor tests, mutant ipt 189 showed y(3hv/prop) = 1.20 g g(-1), a yield very close to the ... | 2000 | 10656331 |
| cloning and expression of vitreoscilla hemoglobin gene in burkholderia sp. strain dnt for enhancement of 2,4-dinitrotoluene degradation. | the gene (vgb) encoding the hemoglobin (vhb) of vitreoscilla sp. was cloned into a broad host range vector and stably transformed into burkholderia (formerly pseudomonas) sp. strain dnt, which is able to degrade and metabolize 2,4-dinitrotoluene (dnt). vgb was stably maintained and expressed in functional form in this recombinant strain (yv1). when growth of yv1, in both tryptic soy broth and minimal salts broth containing dnt and yeast extract, was compared with that of the untransformed strain ... | 2000 | 10662485 |
| prognostic value of cytokine concentrations (tumor necrosis factor-alpha, interleukin-6, and interleukin-10) and clinical parameters in severe melioidosis. | raised serum concentrations of tumor necrosis factor (tnf)-alpha, interleukin (il)-1beta, il-6, or il-10 are associated with mortality in patients with sepsis, but it is not known whether elevated cytokine levels are independently predictive of mortality. cytokine assays (tnf-alpha, il-6, and il-10) were performed on admission plasma samples from 172 adult thai patients with severe melioidosis. mortality was 31.4%. apache ii score; septicemia; plasma lactate; tnf-alpha, il-6, and il-10 concentra ... | 2000 | 10669346 |
| characterization and role of tbux in utilization of toluene by ralstonia pickettii pko1. | the tbu regulon of ralstonia pickettii pko1 encodes enzymes involved in the catabolism of toluene, benzene, and related alkylaromatic hydrocarbons. the first operon in this regulon contains genes that encode the tbu pathway's initial catabolic enzyme, toluene-3-monooxygenase, as well as tbut, the ntrc-like transcriptional activator for the entire regulon. it has been previously shown that the organization of tbut, which is located immediately downstream of tbua1ubva2c, and the associated promote ... | 2000 | 10671442 |
| melioidosis as an emerging global problem. | there is remarkably little known about the incidence of melioidosis outside a few countries (thailand, australia, singapore and malaysia). presumably it is widespread in tropical south east asia. elsewhere there are tantalising glimpses of the tip of what may be a large iceberg. since a specific diagnosis of melioidosis requires awareness on the part of clinicians, and the existence of a laboratory capable of isolating and identifying burkholderia pseudomallei, a luxury not available in most rur ... | 2000 | 10674638 |
| the epidemiology of melioidosis in australia and papua new guinea. | melioidosis was first described in australia in an outbreak in sheep in 1949 in north queensland (22 degrees s). human melioidosis was first described from townsville (19 degrees s) in 1950. melioidosis is hyperendemic in the top end of the northern territory (nt) and as in parts of northeastern thailand it is the commonest cause of fatal community-acquired septicemic pneumonia. in the 9 years since 1989 the prospective nt melioidosis study at royal darwin hospital (12 degrees s) has documented ... | 2000 | 10674639 |
| recent advances in the treatment of severe melioidosis. | for the last decade, high-dose intravenous ceftazidime has been the drug of choice for the treatment of severe melioidosis, after ceftazidime was shown to be superior to the 'conventional' four-drug regimen (chloramphenicol, doxycycline and trimethoprim-sulphamethoxazole) in a randomised trial. combination ceftazidime-trimethoprim-sulphamethoxazole was compared with the conventional regimen in a separate trial with similar results, but we still do not know whether such combination therapy is nee ... | 2000 | 10674641 |
| antimicrobial resistance in burkholderia pseudomallei. | four strains of burkholderia pseudomallei were used to determine the minimum inhibitory concentration (mic), minimum bactericidal concentration (mbc) and time-kill curves with 13 single antimicrobial agents: ceftazidime, piperacillin, imipenem, amoxicillin/clavulanic acid, doxycycline, cotrimoxazole, kanamycin, rifampicin, ciprofloxacin, trovafloxacin, clarithromycin, azithromycin and meropenem. the time-kill studies were also performed with 33 pairs of combinations of the above antimicrobial ag ... | 2000 | 10674642 |
| neurological melioidosis. | neurological abnormalities have long been recognised in animals with melioidosis, including laboratory rodents and sheep in the first australian outbreak in 1949. autopsies in animals have shown microabscesses and lymphocytic infiltration to be present on occasion in the same animal, but burkholderia pseudomallei is usually able to be grown from central nervous system (cns) tissue. in humans cns melioidosis is unusual, but both macroscopic brain abscesses and encephalitis occur. there has been a ... | 2000 | 10674643 |
| animal melioidosis in australia. | melioidosis was first diagnosed in australia in sheep in 1949. while it has been considered endemic in tropical australia, there have been animal outbreaks in southwest western australia and southern queensland. infection occurs in many species, with both latency and a wide range of clinical manifestations. some species may develop melioidosis only if immunocompromised. sheep and goats are particularly susceptible, resulting in the requirement for pasteurisation of tropical commercial goat's mil ... | 2000 | 10674644 |
| ecology of burkholderia pseudomallei and the interactions between environmental burkholderia spp. and human-animal hosts. | early workers thought that melioidosis was a zoonosis with a reservoir in rodents, but we now know that burkholderia pseudomallei is a widely distributed environmental saprophyte. in northeast thailand, two thirds of paddy fields yield the organism, and 80% of children have antibodies by the time they are 4 years old. however, interpretation of these results has been complicated by the recent recognition of avirulent, antigenically cross-reacting environmental organisms for which the name b. tha ... | 2000 | 10674645 |
| multiple replicons constitute the 6.5-megabase genome of burkholderia pseudomallei. | burkholderia pseudomallei is a causative agent of melioidosis, a fatal tropical infectious disease endemic in southeast asia and northern australia. in order to determine the size and characteristics of the bacterial genome, the b. pseudomallei genome and genes were analyzed by pulsed field gel electrophoresis of the undigested, intact megabase dna, and by computational analysis of nucleotide sequences of b. pseudomallei genes which have been sequenced by several investigators and already deposi ... | 2000 | 10674646 |
| molecular phylogeny of burkholderia pseudomallei. | in terms of population structure, the species burkholderia pseudomallei contains both clonal and non-clonal elements. by indexing variation in rrna loci using the restriction endonuclease bamhi, we found that two ribotypes (types 1 and 3) are predominant in nature. ribotype 3 is prevalent in asian countries while ribotype 1 is more widespread. some disease association was suggested for 4 ribotypes and strains of ribotype 4 were markedly associated with a fatal outcome. dna macrorestriction (xbai ... | 2000 | 10674647 |
| genome fingerprinting by pulsed-field gel electrophoresis of isolates of burkholderia pseudomallei from patients with melioidosis in thailand. | a total of 95 isolates of burkholderia pseudomallei from 53 sporadic cases in thailand were examined by pulsed-field gel electrophoresis. digestion of genomic dna of all isolates by ncoi generated a macrorestriction pattern similar to that of b. pseudomallei which cannot assimilate l-arabinose. analysis using restriction enzymes spei and avrii demonstrated greater sensitivity than ncoi digestion in the differentiation of b. pseudomallei and could be used for epidemiological groupings. four clust ... | 2000 | 10674648 |
| use of multiplex pcr patterns as genetic markers for burkholderia pseudomallei. | a simple pcr-based typing method was developed to differentiate between strains of burkholderia pseudomallei. two pairs of primers, based on sequences from two specific dna probes, were used to amplify the bacterial dna by multiplex pcr. we evaluated the pcr method for epidemiological typing of b. pseudomallei and compared this with restriction fragment length polymorphism (rflp) and random amplified polymorphic dna (rapd) methods. in rflp, the dna of b. pseudomallei was digested with hindiii an ... | 2000 | 10674649 |
| pathogenesis of and immunity to melioidosis. | while burkholderia pseudomallei, the causative agent of melioidosis, is becoming increasingly recognized as a significant cause of morbidity and mortality in regions to which it is endemic, no licensed vaccine preparation currently exists for immunization against the disease. therefore, one of the primary goals of our research has been to identify and characterize antigens expressed by b. pseudomallei isolates for the intended purpose of developing a vaccine construct that can be used to activel ... | 2000 | 10674650 |
| exopolysaccharides of burkholderia pseudomallei. | 2000 | 10674651 | |
| protease production by burkholderia pseudomallei and virulence in mice. | the aim of this study was to assess protease production and virulence of various burkholderia pseudomallei strains. protease activity was evaluated in filtrates from cultures grown for 50 h in tsb dialysate by azocasein hydrolysis, and expressed as absorbancy at 405 nm. virulence was assessed in 8 weeks old swiss mice, by intraperitoneal injection of 6-6 x 10(5) cfu, and the ld50 was calculated after 30 days by the method of reed and muench. the lethal activity was studied for five strains of b. ... | 2000 | 10674652 |
| shedding of lipopolysaccharide and 200-kda surface antigen during the in vitro growth of virulent ara- and avirulent ara+ burkholderia pseudomallei. | non-virulent ara+ b. pseudomallei environmental isolates differ from virulent ara- clinical isolates by their ability to assimilate l-arabinose and the absence of a 200 kda antigen on their surface. the latter, present only on the ara- isolates from either clinical or environmental origin, was recently demonstrated by its immunoreactivity with monoclonal antibody (mab) 5f8. we recently demonstrated that lipopolysaccharide (lps) from both biotypes were indistinguishable from one another with rega ... | 2000 | 10674653 |
| proinflammatory cytokine mrna responses in experimental burkholderia pseudomallei infection in mice. | melioidosis is a potentially fatal disease of both human and animals caused by the bacterium burkholderia pseudomallei. disease is endemic in tropical and subtropical regions of southeast asia and northern australia. the pathogenesis of melioidosis is poorly understood. in particular, the host responses that occur following infection, and the specific host-pathogen interactions that result in the development of either acute or chronic infection are unclear. using an established murine model, we ... | 2000 | 10674654 |
| recent developments in laboratory diagnosis of melioidosis. | 2000 | 10674655 | |
| comparison of three pcr primer sets for diagnosis of septicemic melioidosis. | several sets of pcr primers have recently been developed for detection of burkholderia pseudomallei. in this report, the performance of 16s rrna gene primers (16s), rrna spacer gene primers (spacer), and 'lps' primers (lps) were compared. all primer sets were tested by pcr amplification of the same dna samples extracted from blood specimens of 46 patients from northeastern thailand, of which 29 had melioidosis based on blood culture as a gold standard. the sensitivities were 41, 35.7, and 31% wh ... | 2000 | 10674656 |
| bacterial triterpenoids of the hopane series as biomarkers for the chemotaxonomy of burkholderia, pseudomonas and ralstonia spp. | hopanoid fingerprints allowed to differentiate bacteria formerly connected to the genus pseudomonas. whereas all strains related to pseudomonas and ralstonia were devoid of any detectable hopanoid, these pentacyclic triterpenoids were found in the burkholderia species and in related soil isolates, which contained as main hopanoid a bacteriohopanetetrol carbapseudopentose ether, accompanied by significant amounts of its novel delta(6) unsaturated homologue. unsaturated hopanoids represent an extr ... | 2000 | 10675600 |
| interaction between burkholderia pseudomallei and acanthamoeba species results in coiling phagocytosis, endamebic bacterial survival, and escape. | burkholderia pseudomallei causes melioidosis, a potentially fatal disease whose clinical outcomes include rapid-onset septicemia and relapsing and delayed-onset infections. like other facultative intracellular bacterial pathogens, b. pseudomallei is capable of survival in human phagocytic cells, but unlike mycobacteria, listeria monocytogenes, and salmonella serovar typhimurium, the species has not been reported to survive as an endosymbiont in free-living amebae. we investigated the consequence ... | 2000 | 10678988 |
| nebulised taurolidine and b cepacia bronchiectasis. | 2000 | 10681263 | |
| in vitro activity of minocycline against respiratory pathogens from patients with cystic fibrosis. | our objective was to determine the in vitro activity of minocycline against isolates of burkholderia cepacia (bc), stenotrophomonas maltophilia (sm), and pseudomonas aeruginosa (pa) cultured from the respiratory tract of patients with cystic fibrosis (cf). cultures of bc, sm, and pa were isolated in a hospital bacteriology laboratory from the sputum or oropharyngeal cultures obtained from patients attending a cystic fibrosis center, and were prospectively tested for in vitro sensitivity to minoc ... | 2000 | 10686042 |
| response of soybean rhizosphere communities to human hygiene water addition as determined by community level physiological profiling (clpp) and terminal restriction fragment length polymorphism (trflp) analysis. | in this report, we describe an experiment conducted at kennedy space center in the biomass production chamber (bpc) using soybean plants for purification and processing of human hygiene water. specifically, we tested whether it was possible to detect changes in the root-associated bacterial assemblage of the plants and ultimately to identify the specific microorganism(s) which differed when plants were exposed to hygiene water and other hydroponic media. plants were grown in hydroponics media co ... | 2000 | 10689173 |
| procedurally similar competitive immunoassay systems for the serodiagnosis of babesia equi, babesia caballi, trypanosoma equiperdum, and burkholderia mallei infection in horses. | procedurally similar competitive enzyme-linked immunoassay (celisa) methods were developed for the serodiagnosis of babesia equi and babesia caballi (piroplasmosis), trypanosoma equiperdum (dourine), and burkholderia mallei (glanders) infections in horses. apparent test specificities for the b. equi, b. caballi, t. equiperdum, and b. mallei celisas were 99.2%, 99.5%, 98.9%, and 98.9%, respectively. concordances and kappa values between the complement fixation (cf) and the celisa procedures for t ... | 2000 | 10690775 |
| acute melioidosis outbreak in western australia. | a cluster of acute melioidosis cases occurred in a remote, coastal community in tropical western australia. molecular typing of burkholderia pseudomallei isolates from culture-confirmed cases and suspected environmental sources by pulsed-field gel electrophoresis (pfge) of xbai chromosomal dna digests showed that a single pfge type was responsible for five cases of acute infection in a community of around 300 during a 5 week period. this temporal and geographical clustering of acute melioidosis ... | 1999 | 10694154 |
| role of cytosine deaminase and beta-alanine-pyruvate transaminase in pyrimidine base catabolism by burkholderia cepacia. | a determination of the possible role of the salvage enzyme cytosine deaminase or beta-alanine-pyruvate transaminase in the catabolism of the pyrimidine bases uracil and thymine by the opportunistic pathogen burkholderia cepacia atcc 25416 was undertaken. it was of interest to learn whether these enzymes were influenced by cell growth on pyrimidine bases and their respective catabolic products to the same degree as the pyrimidine reductive catabolic enzymes were. it was found that cytosine deamin ... | 2000 | 10696871 |
| identification and population structure of burkholderia stabilis sp. nov. (formerly burkholderia cepacia genomovar iv). | the burkholderia cepacia complex currently comprises five genomic species, i.e., b. cepacia genomovar i, b. multivorans (formerly known as b. cepacia genomovar ii), b. cepacia genomovar iii, b. cepacia genomovar iv, and b. vietnamiensis (also known as b. cepacia genomovar v). in the absence of straightforward diagnostic tests for the identification of b. cepacia genomovars i, iii, and iv, the last two genomic species were not formally classified as novel burkholderia species (genomovar i contain ... | 2000 | 10698993 |
| [various genetic and molecular and biologic aspects of the melioidosis causative organism]. | 2000 | 10702990 | |
| novel regioselective hydroxylations of pyridine carboxylic acids at position c2 and pyrazine carboxylic acids at position c3. | we have previously described the isolation of the new bacterial species, ralstonia/burkholderia sp. strain dsm 6920, which grows with 6-methylnicotinate and regioselectively hydroxylates this substrate in the c2 position by the action of 6-methylnicotinate-2-oxidoreductase to yield 2-hydroxy-6-methylnicotinate (tinschert et al. 1997). in the present study we show that this enzymatic activity can be used for the preparation of a series of hydroxylated heterocyclic carboxylic acid derivatives. the ... | 2000 | 10709981 |
| the dsba-dsbb disulfide bond formation system of burkholderia cepacia is involved in the production of protease and alkaline phosphatase, motility, metal resistance, and multi-drug resistance. | in a previous study, we isolated a dsbb mutant of burkholderia cepacia kf1 and showed that phenotypes of protease production and motility are dependent on dsbb, a membrane-bound disulfide bond oxidoreductase. we have now isolated a dsba mutant by transposon mutagenesis, cloned the dsba gene encoding a periplasmic disulfide bond oxidoreductase, and characterized the function of the dsba-dsbb disulfide bond formation system in b. cepacia. the complementing dna fragment had an open reading frame fo ... | 2000 | 10711598 |
| production of n-acyl-l-homoserine lactones by p. aeruginosa isolates from chronic lung infections associated with cystic fibrosis. | the n-acyl-l-homoserine lactones (ahls) produced by sequential pseudomonas aeruginosa isolates from chronically infected patients with cystic fibrosis were analyzed by thin-layer chromatography. it is demonstrated that both the amounts and the types of molecules synthesized by isolates from patients who were monitored over periods of up to 11 years do not change significantly during chronic colonization. however, in the case of a patient who became co-infected with an ahl-producing burkholderia ... | 2000 | 10713433 |
| melioidosis presenting as urinary tract infection in a previously healthy tourist. | melioidosis is a tropical disease caused by burkholderia pseudomallei, which is common in southeast asia and australia, but which is rarely diagnosed in scandinavia. an increasing number of cases are being reported among tourists to infected areas. we report the first finnish case of melioidosis, which presented as urinary tract infection in a previously healthy male tourist. | 2000 | 10716086 |
| differential antibiotic-induced endotoxin release in severe melioidosis. | severe melioidosis is a life-threatening, systemic bacterial infection caused by burkholderia pseudomallei. a prospective, randomized treatment trial was conducted in northeast thailand to compare ceftazidime (a penicillin-binding protein [pbp]-3-specific agent that causes release of large amounts of endotoxin in vitro) and imipenem (a pbp-2-specific agent that kills b. pseudomallei more rapidly but releases low amounts of endotoxin) in severe melioidosis over a 6-h time course after the first d ... | 2000 | 10720525 |
| cable-piliated burkholderia cepacia binds to cytokeratin 13 of epithelial cells. | although the burkholderia cepacia complex consists of several genomovars, one highly transmissible strain of b. cepacia has been isolated from the sputa of cystic fibrosis (cf) patients throughout the united kingdom and canada. this strain expresses surface cable (cbl) pili and is thought to be the major strain associated with the fatal "cepacia syndrome." in the present report we characterize the specific 55-kda buccal epithelial cell (bec) protein that binds cable pilus-positive b. cepacia. n- ... | 2000 | 10722565 |
| cytokine gene expression in innately susceptible balb/c mice and relatively resistant c57bl/6 mice during infection with virulent burkholderia pseudomallei. | production of cytokines including gamma interferon (ifn-gamma) and tumor necrosis factor alpha (tnf-alpha) is an important early-stage host response following infection with intracellular pathogens. development of immunity to these pathogens is determined to a large extent by the timing and relative level of expression of the cytokines. numerous studies have shown that early cytokine responses involving interleukin-12 (il-12) and ifn-gamma are important for resistance to intracellular pathogens, ... | 2000 | 10722599 |
| xanthine oxidase contributes to host defense against burkholderia cepacia in the p47(phox-/-) mouse model of chronic granulomatous disease. | chronic granulomatous disease (cgd) is an inherited disorder of the nadph oxidase in which phagocytes are defective in generating superoxide and downstream microbicidal reactive oxidants, leading to recurrent life-threatening bacterial and fungal infections. xanthine oxidase (xo) is another enzyme known to produce superoxide in many tissues. using the p47(phox-/-) mouse model of cgd, we evaluated the residual antibacterial activity of xo. clearance of burkholderia cepacia, a major pathogen in cg ... | 2000 | 10722648 |
| use of oligotrophic bacteria for the biological monitoring of heavy metals. | oligotrophic bacteria exhibited active growth even in nutritionally deficient medium made with nutrient broth that had been diluted with distilled water, 1 : 10 000. the oligotrophic bacteria, sphingomonas paucimobilis kps01 and burkholderia cepacia kpc01 and kpc02 were found to be highly susceptible to heavy metals and to be potentially useful as sensors for the assessment of toxicity. the susceptibility of the bacteria to metals was measured by incubating the bacteria with metals of varying co ... | 2000 | 10735254 |
| synthesis of regioselectively protected forms of cytidine based on enzyme-catalyzed deacetylation as the key step. | n4-acetylcytidine (77%) and 2',3'-o, n4-triacetylcytidine (95%) were obtained from the hydrolysis of a common precursor, the peracetylated form of cytidine with aspergillus niger lipase (amano a) and burkholderia cepacia esterase (sc esterase s), respectively, under very mild conditions. the experimental procedure for the conversion of triacetylcytidine to a corresponding phosphoramidite (82%), an intermediate for sugar nucleotide synthesis, is also elaborated. | 2000 | 10737194 |
| involvement of two plasmids in fenitrothion degradation by burkholderia sp. strain nf100. | a bacterium capable of utilizing fenitrothion (o,o-dimethyl o-4-nitro-m-tolyl phosphorothioate) as a sole carbon source was isolated from fenitrothion-treated soil. this bacterium was characterized taxonomically as being a member of the genus burkholderia and was designated strain nf100. nf100 first hydrolyzed an organophosphate bond of fenitrothion, forming 3-methyl-4-nitrophenol, which was further metabolized to methylhydroquinone. the ability to degrade fenitrothion was found to be encoded on ... | 2000 | 10742273 |
| neck lumps: expect the unexpected. | 2000 | 10744094 | |
| characterization of three capsular polysacharides produced by burkholderia pseudomallei. | three kinds of capsular polysaccharide (cp) were found to be produced by burkholderia pseudomallei. when the bacterium was grown with the medium without glycerol, cp-1a and cp-1b were produced. cp-1a was mainly 1.4-linked glucan and cp-1b was identified as a polymer composed of galactose and 3-deoxy-d-manno-octulosonic acid, whose chemical structure was recently reported by other laboratories. when the bacterium was grown with the medium containing 5" glycerol. cp-2 was synthesized. cp-2 contain ... | 1998 | 10744478 |
| survival and growth of burkholderia cepacia within the free-living amoeba acanthamoeba polyphaga. | the aim of this study was to investigate whether burkholderia cepacia is capable of survival and growth within the free-living amoeba acanthamoeba polyphaga using a differential immunofluorescence assay of bacterial-amoebal cocultures and viable counts of bacteria determined after amoebal lysis. the numbers of intra-amoebal bacteria and the numbers of infected amoebae increased over time; although, when heat-killed bacteria were used, no intracellular bacteria were observed. these findings shoul ... | 2000 | 10746499 |
| molecular typing and exopolysaccharide biosynthesis of burkholderia cepacia isolates from a portuguese cystic fibrosis center. | this work describes the first epidemiological survey of burkholderia cepacia involved in pulmonary infections among the portuguese population with cystic fibrosis (cf) who attended the major cf treatment center in lisbon at sta. maria hospital from 1995 to the end of 1997. the characterization of the genomic relatedness of the isolates was based on the analysis of their ribopatterns (with ecori) followed by construction of a ribotype-based phylogenetic tree. this study was complemented with macr ... | 2000 | 10747161 |
| evaluation of a new commercially available immunoglobulin m and immunoglobulin g immunochromatographic test for diagnosis of melioidosis infection. | an immunochromatographic test for the rapid determination of immunoglobulin m (igm) and igg antibodies to burkholderia pseudomallei was evaluated by using sera from bacteriologically confirmed melioidosis patients and high-risk and clinically suspected patients, along with disease control groups. the sensitivities were 100 and 93% for the igg and igm tests, respectively, while the specificity was 95% for both assays. the test was rapid and simple to perform, with results obtained in 10 min. | 2000 | 10747166 |
| in vitro antibacterial spectrum of a new broad-spectrum 8-methoxy fluoroquinolone, gatifloxacin. | the in vitro antibacterial spectrum of gatifloxacin was compared with those of ciprofloxacin and ofloxacin. gatifloxacin was two- to four-fold more potent than comparator quinolones against staphylococci, streptococci, pneumococci and enterococci (gatifloxacin mic90s, < or =1 mg/l, except 4 mg/l against methicillin-resistant staphylococcus aureus and enterococcus faecium). gatifloxacin was two-fold less potent than ciprofloxacin, and the same as or two-fold more potent than ofloxacin against ent ... | 2000 | 10747819 |
| endobronchial mass in a patient with burkholderia pseudomallei infection. | burkholderia pseudomallei infection, the great mimicker of infectious diseases, has protean clinical manifestations. | 2000 | 10748977 |
| enzymatic desymmetrization of a meso polyol corresponding to the c(19)-c(27) segment of rifamycin s. | the stereoselective acylation of meso polyol 2 by vinyl acetate (solvent and acyl donor) in the presence of porcine pancreas lipase gave the corresponding monoester 5 in good yield (76%) and high enantiomeric purity (ee > 98%). the enzymatic reaction was also highly regioselective for a primary alcohol end group, and the two unprotected secondary alcohols were not involved. compound 5 corresponds to the c(19)-c(27) fragment of rifamycin s. | 2000 | 10750492 |
| highly resistant burkholderia pseudomallei small colony variants isolated in vitro and in experimental melioidosis. | burkhloderia pseudomallei is the causative agent of melioidosis, a disease in which treatment failures and relapses are common. this study reports on slow growing b. pseudomallei 'small colony variants' (scvs), isolated either in vitro after exposure to ceftazidime, ciprofloxacin or gentamicin or from the spleen and liver in a mouse model of melioidosis after treatment with ceftazidime. interestingly, scvs isolated by either method or antimicrobial agent showed a significant increase in the mini ... | 1999 | 10753061 |
| exopolysaccharide production by mucoid and non-mucoid strains of burkholderia cepacia. | thirteen strains of burkholderia cepacia from various origins with mucoid and non-mucoid phenotypes were assayed for exopolysaccharide (eps) production. the eps were characterized by glycosyl composition analysis and examination of the products resulting from lithium-ethylenediamine and smith degradations. the results showed that all strains, including the non-mucoid strains, were able to produce eps exhibiting the same structural features, i.e. presence of one rhamnosyl, three galactosyl, one m ... | 2000 | 10754255 |
| a de novo synthesis of 2,6-dideoxy-c-aryl glycosides based on ring closing metathesis and diastereoselective epoxide cleavage/anomerization reactions. | [formula: see text] this paper describes a synthesis of enantiomerically pure 2,6-dideoxy-c-aryl glycosides, starting from non-carbohydrate precursors. the synthesis starts from homoallylic alcohols (obtained in enantiomerically pure form by enzymatic resolution), which are elaborated to dihydropyrans using ring closing metathesis as the key step. epoxidation and epoxide cleavage complete the synthesis. the stereochemical outcome of the sequence depends on the conditions of the epoxide cleavage ... | 2000 | 10754682 |
| application of membrane filtration for removal of diminutive bioburden organisms in pharmaceutical products and processes. | in this report, we present results of a recent investigation in our laboratories demonstrated the effect of process conditions and/or drug product composition on the ability of 0.2 micron and 0.22 micron sterilizing grade filters to fully retain ralstonia (formerly burkholderia, formerly pseudomonas) pickettii. r. pickettii is a opportunistic pathogen widely distributed in nature as well as clinical specimens and there have been several reports of nosocomial infections due to intrinsic manufactu ... | 1999 | 10754712 |
| biodegradation of phenol by bacterial strains from petroleum-refining wastewater purification plant. | the ability of four strains of bacteria derived from a biological petroleum-refining wastewater purification plant to carry out the biodegradation of phenol was studied. two of the strains belonging to the genus pseudomonas were found to be characterised by high effectiveness of the removal of phenol which was used as sole carbon and energy source (the strains were designated p1 and p2). in turn the effect of inoculum size, initial concentration of substrate (500 and 1,000 mg phenol/l) and tempe ... | 1999 | 10756720 |
| application of solid-phase microextraction-gas chromatography-mass spectrometry to characterize intermediates in a joint solar-microbial process for total mineralization of aroclor 1254. | a combined solid-phase microextraction-gc-ms analytical technique was used to monitor the formation of metabolites in the biodegradation of biphenyl, which were originally obtained from the solar photodechlorination of aroclor 1254 by pseudomonas pseudoalcaligenes kf707 and burkholderia sp lb400. in both cases, the following metabolites were detected: 2-hydroxybiphenyl (2-oh-bp), 2,3-dihydroxybiphenyl (2,3-di-oh-bp), and benzoic acid, which was detected as its benzoate derivative 1-methylethylbe ... | 2000 | 10757284 |
| burkholderia kururiensis sp. nov., a trichloroethylene (tce)-degrading bacterium isolated from an aquifer polluted with tce. | a trichloroethylene (tce)-degrading bacterium was isolated from an aquifer sample collected at a tce-polluted site in japan by enriching with phenol as sole carbon source. the isolate, designated strain kp23t, was a gram-negative, oval-shaped micro-organism. a phylogenetic study based on 16s rrna gene sequences indicated that strain kp23t should be placed in the genus burkholderia. cellular fatty acids of the strain were mainly composed of c16:0, cyclopropanic acid c17:0 and cyclopropanic acid c ... | 2000 | 10758884 |
| description of pandoraea gen. nov. with pandoraea apista sp. nov., pandoraea pulmonicola sp. nov., pandoraea pnomenusa sp. nov., pandoraea sputorum sp. nov. and pandoraea norimbergensis comb. nov. | a polyphasic taxonomic study was performed on a group of isolates tentatively identified as burkholderia cepacia, ralstonia pickettii or ralstonia paucula (formerly known as cdc group ivc-2). the isolates were mainly cultured from sputum of cystic fibrosis patients or from soil. sds-page of whole-cell proteins and aflp fingerprinting distinguished at least five different species, and this was confirmed by dna-dna hybridizations. 16s rdna sequence analysis of representative strains indicated that ... | 2000 | 10758901 |
| optical resolution of (+/-)-1-aryl-1-alkanols using enantioselective transesterification by lipases. | enantioselective transesterification of 1-phenyl-1-alkanols (phch(oh)(ch2)n-2ch3; n = 2, 3, 4, 5, 6, 9, 12, 18) with vinyl acetate catalyzed by lipases in benzene has been studied to find the catalyst which is generally used for preparing optically active 1-phenyl-1-alkanols having various alkyl chains. amongst lipases examined (lipases lip, ps, ak, cal and rml), the lipase from pseudomonas aeruginosa (lip) is the best catalyst which shows high reactivity and enantioselectivity and low substrate ... | 2000 | 10763870 |
| multiple combination bactericidal antibiotic testing for patients with cystic fibrosis infected with burkholderia cepacia. | most burkholderia cepacia strains are resistant to many, or all, of the antibacterial agents commonly used in cystic fibrosis (cf), and selection of appropriate antibiotics for treatment of pulmonary exacerbations is therefore difficult. we developed a technique for rapid in vitro testing of multiple antibiotic combinations for b. cepacia isolates. for each of 119 multi-drug-resistant isolates of b. cepacia, our multiple combination bactericidal test (mcbt) studied the bactericidal activity of 1 ... | 2000 | 10764313 |
| interaction of insulin with burkholderia pseudomallei may be caused by a preservative. | to re-examine the previously reported in vitro interaction of insulin with burkholderia pseudomallei, in the light of a suggestion that the interaction may have resulted from the presence of the preservative m-cresol in commercial preparations. | 2000 | 10767836 |
| histopathologic features of burkholderia cepacia pneumonia in patients without cystic fibrosis. | we present the histopathologic features of fatal burkholderia cepacia pneumonia in three adults (one man [age 44 years] and two women [aged 40 and 43 years]). in all patients, the pulmonary infiltrates initially were localized (right middle lobe, left upper lobe, and right middle lobe) but rapidly progressed. two open-lung biopsies and one pneumonectomy specimen showed necrotizing granulomatous inflammation merging with areas of more conventional necrotizing bronchopneumonia in one patient, a me ... | 2000 | 10786801 |
| quantification of phnac and nahac in contaminated new zealand soils by competitive pcr. | unculturable polycyclic aromatic hydrocarbon (pah)-degrading bacteria are a significant reservoir of the microbial potential to catabolize low-molecular-weight pahs. the population of these bacteria is larger than the population of nah-like bacteria that are the dominant organisms in culture-based studies. we used the recently described phn genes of burkholderia sp. strain rp007, which feature only rarely in culture-based studies, as an alternative genotype for naphthalene and phenanthrene degra ... | 2000 | 10788344 |
| aerobic degradation of dinitrotoluenes and pathway for bacterial degradation of 2,6-dinitrotoluene. | an oxidative pathway for the mineralization of 2,4-dinitrotoluene (2, 4-dnt) by burkholderia sp. strain dnt has been reported previously. we report here the isolation of additional strains with the ability to mineralize 2,4-dnt by the same pathway and the isolation and characterization of bacterial strains that mineralize 2, 6-dinitrotoluene (2,6-dnt) by a different pathway. burkholderia cepacia strain js850 and hydrogenophaga palleronii strain js863 grew on 2,6-dnt as the sole source of carbon ... | 2000 | 10788393 |
| comparative evaluation of the in vitro activity of three combinations of beta-lactams with beta-lactamase inhibitors: piperacillin/tazobactam, ticarcillin/clavulanic acid and ampicillin/sulbactam. | recently, two new combinations of beta-lactam antibiotics with beta-lactamase inhibitors became commercially available in brazil: piperacillin/tazobactam and ampicillin/sulbactam. this study was designed to assess and compare the in-vitro activity of these new compounds, as well as that of ticarcillin/clavulanic acid, against bacteria isolated in our environment. a total of 749 bacteria isolated at são paulo hospital were tested using the disk diffusion method, in compliance with nccls standardi ... | 2000 | 10788842 |