Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| protein changes in skeletal muscle of toad, bufo melanostictus under cathode polarity treatment. | 1988 | 3264268 | |
| the effect of bovine parathormone on ultimobranchial body, parathyroid gland and paravertebral lime sacs as correlated with serum calcium and inorganic phosphorus levels in bufo melanostictus schneider. | 1988 | 3234570 | |
| polarized electric field effects on the regulation of succinate dehydrogenase activity in amphibian muscle and liver: kinetic study. | electropolarity treatment (0.8 v/dc/cm) was given to the gastrocnemius muscle of bufo melanostictus every day for 5 min. for 5 days, and kinetic study of succinate dehydrogenase (sdh) in muscle and liver was conducted with different effectors - sodium malonate, ethylene diamine tetra acetic acid (edta), calcium chloride (cacl2) and sodium citrate. of the four modulators tested, the malonate and edta inhibited while sodium citrate and cacl2 activated the enzyme. the significance of the modulation ... | 1989 | 2504168 |
| effect of corticosteroids on carbohydrate metabolism in bufo melanostictus (schneider). | a single injection of corticosterone (1 or 5 micrograms/50 g body weight) produced a significant elevation in plasma glucose, liver and muscle glycogen contents of b. melanostictus. single but identical doses of aldosterone had no effect on plasma glucose concentration. liver and muscle glycogen contents were however significantly augmented. administration of 1 or 5 micrograms corticosterone and 1 microgram or 200 ng aldosterone/50 g body weight, for 15 days, caused no change in plasma glucose c ... | 1989 | 2633967 |
| glycogen utilization in tissues of toad, bufo melanostictus under electropolarity treatment. | electropolarity treatment (0.8v/dc/cm) was given to the gastrocnemius muscle of b. melanostictus every day for 5 min for 5 days. the glycogen content and aldolase activity levels increased and phosphorylase 'a' activity levels decreased on cathode treatment in muscle, liver and brain while an opposed trend was observed on anode treatment. the heart showed contrasting pattern under both cathode and anode electropolarity treatments. | 1989 | 2606527 |
| effect of triiodothyronine and thyroxine on malic enzyme activity and protein and nucleic acid contents of liver and muscle of toad, bufo melanostictus. | 1989 | 2466749 | |
| effect of lithium chloride on spermatogenesis and testicular delta 5-3 beta, 17 beta-hydroxysteroid dehydrogenase activities in the 'antiserum to luteinizing hormone' treated toad (bufo melanostictus). | activities of delta 5-3 beta- and 17 beta-hydroxysteroid dehydrogenase (delta 5-3 beta-hsd and 17 beta-hsd), leydig cell nuclear area (lcna) and spermatogenesis in the testis were observed after injection of lithium chloride in the 'antiserum to luteinizing hormone (lh)' treated toad. a significant decrease in the activities of steroidogenic enzymes, lcna and spermatogenesis were noticed after the injections of 'antiserum to lh' to toads. further decrease in the activities of the above parameter ... | 1990 | 2177728 |
| influence of pituitary on histology of bidder's organ in castrated toad bufo melanostictus. | weight, number of follicles and corpora lutea of bidder's organ increased significantly after castration of adult toad in breeding season. removal of pituitary along with testes decreased both weight and number of follicles and corpora lutea. administration of luteinizing hormone (lh) in castrated and hypophysectomized toad showed more or less similar result as that of control indicating that lh has an effect on the histology of bidder's organ in the toad. | 1990 | 2253975 |
| effect of electropolarity treatment on activity levels of oxidative enzymes in gastrocnemius muscle of toad, bufo melanostictus, during early stages of denervation. | the activity levels of succinate dehydrogenase (sdh), malate dehydrogenase (mdh) and cytochrome-c-oxidase showed a decrement whereas lactate dehydrogenase (ldh) evinced maximum activity during first 3 days of denervation. under the impact of cathodal polarity treatment an elevation in the activity levels of these 4 enzymes was relatively more potent when compared to anodal polarity treatment. the role of polarity treatment in the regulation of these oxidative enzymes was discussed. | 1990 | 2169460 |
| substrate-affinity relation of succinate and lactate dehydrogenases in tissues of toad, bufo melanostictus under electropolarity treatment. | the kinetic analysis of succinate and lactate dehydrogenases (sdh and ldh) has been studied in muscle and liver of bufo melanostictus under electropolarity treatment. increased vmax and decreased km were observed both in liver and muscle under cathode electropolarity treatment whereas a reverse trend was noticed under anodal treatment. but the activation energy values (delta e) of both the enzymes decreased indicating enhanced catalytic efficiency under both cathodal and anodal treatments. | 1990 | 2353919 |
| thyroid hormone effects upon na+k+ dependent adenosine triphosphatase activity of microsomal fraction of liver, muscle and brain of toad bufo melanostictus. | na+k(+)-atpase activity in the liver and muscle microsomal membranes have been determined by different doses (0.1, 0.25, 0.5, 1 and 2 micrograms/gm of body weight) of l-triiodothyronine and l-thyroxine in the toad, bufo melanostictus. the minimum effective dose of t3 was 0.5 microgram/g in case of both liver and muscle to stimulate the enzyme activity and there was dose dependent rise between t3 at the doses of 0.5 and 1 microgram/g. t3 at the doses of 1 and 2 micrograms/g produced more or less ... | 1990 | 1966031 |
| induction of thyroid hormone in changing na+k(+)-atpase activity in different organs of toad, bufo melanostictus. | single injection of t3, at the doses of 0.5 and 1 micrograms/g body weight, stimulated na+k(+)-atpase activity of crude liver homogenate of toad in a dose dependent fashion. lowering the dose of t3 to 0.25 micrograms/g had no effect on the enzyme activity. t3 at the dose of 2 micrograms/g showed the same level of enzyme activity at par with that of 1 microgram/g. na+k(+)-atpase activity of muscle increased with t3 at the doses of 1 and 2 micrograms/g, but without any dose dependent manner while ... | 1990 | 2173671 |
| effect of triiodothyronine and thyroxine on mitochondrial alpha-glycerophosphate dehydrogenase activity and mitochondrial protein content of liver, muscle and brain of toad, bufo melanostictus. | the responsiveness of the adult toad to triiodothyronine (t3) and thyroxine (t4) was studied by measuring the mitochondrial alpha-glycerophosphate dehydrogenase activity and mitochondrial protein content of liver, muscle and brain of toad. both t3 and t4 increased the alpha-gpd activity and mitochondrial protein content of liver and muscle of toad. the extent of increase in the alpha-gpd activity and mitochondrial protein content were more pronounced with t3 than with t4. further that the muscle ... | 1990 | 2227800 |
| effect of estradiol on spermatogenesis and testicular hydroxysteroid dehydrogenase activities in bidder's organectomized toad (bufo melanostictus). | inhibition of spermatogenic activity and increase in leydig cell nuclear area (l c n a), testicular delta 5-3 beta-hydroxysteroid dehydrogenase (delta 5-3 beta-hsd) and 17 beta-hydroxysteroid dehydrogenase (17 beta-hsd) activities were noted after bidder's organectomy. administration of estradiol in bidder's organectomized toad showed more or less similar result as the control animal. it is supposed that estradiol coming from the bidder's organ might play a role in normal testicular activities. | 1991 | 1950593 |
| comparative effects of mast cell degranulators on perfused systemic blood vessels of bufo melanostictus and rana tigrina. | three agents known to induce release of mast cell constituents, viz. polymyxin, compound 48/80 and polysorbate-80, were evaluated for effect on perfused blood vessels of r. tigrina and b. melanostictus. the mast cell degranulators caused vasoconstriction in frog and toad, except that for p-80 whose responses in toad were equivocal. toads showed a general low responsiveness in comparison to frogs. pharmacologic intervention with pheniramine, metergoline, hydergine, atropine and mecamylamine, resp ... | 1992 | 1338198 |
| receptor mechanisms in vasoconstrictor responses to choline esters in bufo melanostictus. | choline esters have been found to cause vasoconstriction of perfused systemic blood vessels of common indian toad, bufo melanostictus. the vasoconstriction appears to be mediated through atropine sensitive receptors and nicotinic mechanisms appear unlikely to be involved. | 1992 | 1473849 |
| histochemical study on adrenal delta 5-3 beta-hydroxysteroid dehydrogenase activity in cadmium treated toad (bufo melanostictus). | effect of a single subcutaneous injection of cadmium chloride at the dose of 0.5 mg/toad on adrenal delta 5-3 beta-hydroxysteroid dehydrogenase (delta 5-3 beta-hsd) was observed after 7 days. the activity of delta 5-3 beta-hsd was measured histochemically. the experiments indicate that cadmium chloride resulted in a significant decrease in the activity of adrenal delta 5-3 beta-hsd in toad during breeding season (june-july). | 1992 | 1288058 |
| a scanning electron microscopic study of the oviduct of the toad, before and during ovulation. | the surface features of the oviduct of the toad, bufo melanostictus, was studied under the scanning electron microscope. the epithelial folds show regional variations, being longitudinally disposed in the upper part of the oviduct, spirally arranged in the middle and convoluted in the lower part. there is an abundance of cilia through the oviduct except in the ovisac. goblet cells are interspersed among the epithelial cells. in between the epithelial folds are openings of tubular secretory gland ... | 1993 | 8443422 |
| effect of histaminergic drugs on tail melanophores of tadpole, bufo melanostictus. | the h1 and h2 receptor agonist histamine caused a powerful aggregation of b. melanostictus tail melanophores, which was completely blocked by metiamide, a specific h2 receptor antagonist, while mepyramine an h1 receptor blocker partially blocked the aggregating response. the strong melanin aggregating effect of 4-methyl histamine a specific h2 receptor agonist and its complete blockade by metiamide further supports the conclusion that there exists a dominant population of h2 type of histamine re ... | 1993 | 8359851 |
| hydroquinone simultaneously induces indole amine 2,3 dioxygenase (iod) and inhibits tyrosinase in bufo melanostictus. | hydroquinone administration in bufo melanostictus causes an induction of indole amine 2,3-dioxygenase in skin and liver while simultaneously inhibiting tyrosinase in these organs. the result is discussed with the reports of higher urinary excretion of abnormal indole metabolites including derivatives of anthranilic acid in vitiliginous subjects (roychoudhury and chakraborty, clinica. chim. acta 22, 298, 1968; kurbanov and berezov, vopr. med. khim. 22, 683, 1976). | 1993 | 8502114 |
| impact of electropolarity treatment on contractile kinetics of gastrocnemius muscle of toad (bufo melanostictus) during denervation. | the twitch properties of denervated muscle were found to be altered contributing to the derangement in the contractile machinery of the denervated muscle. cathodal polarity treatment minimized the fluctuations and tried to restore denervated muscle back to normalcy to some extent. the anodal polarity treatment was not able to produce favourable effect. the differential effect of polarity treatment on the contractile kinetics of the denervated muscle is discussed. | 1993 | 8500819 |
| effect of vitamin a excess on oral armature in tadpoles of toad, bufo melanostictus (schneider) and frog, rana cyanophlyctis (schneider). | tadpoles of b. melanostictus and r. cyanophlyctis were reared in vitamin a solution. rearing of the tadpoles of b. melanostictus in solutions containing 15, 20 and 30 iu/ml vitamin a palmitate resulted in complete disappearance of the keratinized epidermal material over the jaws and horny labial teeth. however tadpoles of the frog r. cyanophlyctis, were not affected in this way. | 1994 | 7959930 |
| effect of lithium chloride on testicular delta 5-3 beta, 17 beta-hydroxy steroid dehydrogenase, acid phosphatase and gametogenesis in bufo melanostictus. | injection of lithium chloride at the dose of 200 micrograms/toad/alternate day for 7, 14 and 21 days caused a significant reduction in the activities of testicular delta 5-3 beta, 17 beta-hydroxysteroid dehydrogenase and acid phosphatase enzymes. there was a marked inhibition in spermatogenesis in lithium chloride treated toad for 14 and 21 days of treatment, but 7 days of treatment has no effect. | 1994 | 7927527 |
| mucopolysaccharide histochemistry of the oviduct of the toad, bufo melanostictus, before and during ovulation. | in both non-ovulating and ovulating toads of the species, bufo melanostictus, the fimbrium stained only weakly for mucopolysaccharides (mps) whereas the infundibulum stained strongly for neutral mps and also for glycogen. in the non-ovulating toad, only neutral and sulphated mps were detected in the goblet cells of the upper magnum, whereas sulphated, neutral and sialomucins were detected in the glands. in the middle magnum, sulphated and sialic acid-containing carboxylated mps were detected in ... | 1994 | 7516206 |
| isolation, purification and partial characterization of a haemolytic protein from bidder's organ of toad bufo melanostictus (schneider). | a haemolytic protein toxin (bo-ht) from bidder's organ of toad, b. melanostictus, purified by deae-cellulose column chromatography was electrophoretically homogeneous and was glycoprotein in nature (pas-positive). the molecular weight was estimated to be 14.4 kda by sds-polyacrylamide gel electrophoresis. the sensitivity of the haemolysin of different rbc ghost cell preparation was in the order: buffalo > goat > ox > guinea pig > mice > human > chick > rabbit > rat. the haemolytic activity was i ... | 1994 | 8045611 |
| in vivo and in vitro effects of metabolic hormones on tissue respiration in toad, bufo melanostictus during hibernation and active phase. | in vivo and in vitro effects of l-t3, l-t4, estradiol, corticosterone, epinephrine and norepinephrine were studied on the rate of liver and muscle tissue respiration in b. melanostictus during hibernation and active phase under natural climatic conditions. thyroid hormones were found to be calorigenic only during the active phase. estradiol stimulated the respiratory rate directly during hibernation and indirectly during the active phase. adrenal hormones invariably increased the respiratory rat ... | 1995 | 8543328 |
| role of cholinergic receptors in melanophore responses of amphibians. | responses of isolated skin melanophores of rana tigerina and bufo melanostictus to cholinergic drugs were studied using the mean melanophore size index assay to explore the nature and role of cellular receptors in melanophore regulation activity. acetylcholine (ach) in a concentration of 10(-7) to 6.4 x 10(-6) g/ml caused dispersion of the skin melanophores of r. tigerina and b. melanostictus. these effects were blocked by both atropine and hyoscine in a concentration of 8 x 10(-6) g/ml each. es ... | 1995 | 8714764 |
| histophysiological evidence for parathyroid mediation of estrogen- and prolactin-induced hypercalcemia in the toad bufo melanostictus (schneider). | administration of estrogen as well as prolactin to b. melanostictus resulted in hypercalcemia. parathyroids and ultimobranchials of the toad were hypertrophied and the secretory epithelium reflected hyperplasia. histophysiological manifestation of the parathyroids of treated toads suggests that estrogen and prolactin bring about hypercalcemia by stimulating the parathyroids. changes in the ultimobranchials suggest hyperactivity of the gland to combat estrogen- and prolactin-induced hypercalcemia ... | 1995 | 8588504 |
| vitiligo, psoralen, and melanogenesis: some observations and understanding. | since the etiology of vitiligo is still unknown, we searched for some abnormal biochemical parameters, if any, in subjects with vitiligo. higher urinary excretion of indole metabolites in vitiliginous patients have been noted, in association with higher dioxygenase, superoxide dismutase, and tyrosine aminotransferase activity in their serum. similar results have also been found in an animal model, bufo melanostictus, during induced tyrosinase inhibition. treatment with psoralen can reverse the p ... | 1996 | 8888309 |
| effect of histaminergic drugs on integumental melanophores of adult bufo melanostictus. | histamine and 2-methyl histamine caused dose-dependent aggregation of melanophores in toad b. melanostictus. the effects were effectively antagonised by mepyramine, a specific h1 histamine receptor antagonist, and metiamide a specific h2 receptor antagonist. on the other, hand 4-methyl histamine, a specific h2 receptor agonist dispersed the melanophores. the results suggest that adult bufo melanophores have h1 histamine receptors which mediate melanophore aggregation, however, dispersion of mela ... | 1996 | 9063075 |
| a lethal cardiotoxic protein isolated from bidder's organ of common indian toad, bufo melanostictus schneider. | a lethal cardiotoxic (bo-ct; bidder's organ cardiotoxin) protein was purified from the bidder's organ of the common indian toad b. melanostictus by gel filtration on sephadex g-200. the homogeneity of cardiotoxin was tested by gel electrophoresis. the molecular weight of lethal bo-ct was 62 kda and was devoid of glycoprotein. ld50 of the bo-ct was 50 micrograms/20 g (i.v.) in male albino mice. on isolated heart and auricle bo-ct initially increased the rate and amplitude of contraction and final ... | 1996 | 8781032 |
| haematological characteristics and bone fluoride content in bufo melanostictus from an aluminium industrial site. | fluoride concentration in bones and differential haemotological characteristics (rbc, haemoglobin, haematocrit, mean corpusclar haemoglobin and mean corpuscular volume) were measured in amphibians, bufo melanostictus, collected from fluoride-contaminated and -uncontaminated areas. the average haemoglobin content, total rbc count and haematocrit (%) in blood samples were found to be significantly reduced, while mean corpuscular concentration and volume were significantly elevated in individuals f ... | 1998 | 15093307 |
| direct effects of lithium chloride on the activities of delta 5-3 beta and 17 beta-hydroxysteroid dehydrogenase in the testis and bidder's organ of the adult toad (bufo melanostictus)--in vitro study. | steroidogenic key enzymes, i.e. delta 5-3 beta and 17 beta-hydroxysteroid dehydrogenase (delta 5-3 beta and 17 beta-hsd) activities, in the testis and bidder's organ of the toad were inhibited and ascorbic acid synthesis in these organs was decreased by a wide range of lithium concentration in in vitro study. a significant inhibition was noted at a concentration of 2.0 mm, which is easily achieved in the blood during the treatment of manic patients by lithium chloride. this experiment reflected ... | 1999 | 10754790 |
| the ontogeny of kin recognition in tadpoles of the toad bufo melanostictus (anura; bufonidae). | the ontogeny of kin recognition and influence of social environment on the development of kin recognition behaviour was experimentally investigated in tadpoles of bufo melanostictus that lived in aggregations and showed low larval dispersion. embryos and tadpoles of the toad were reared as (i) kin only, (ii) with kin and non-kin (separated by a mesh screen), and (iii) in isolation. they were tested for the ability to discriminate between (i) familiar siblings and unfamiliar non-siblings, (ii) fa ... | 2000 | 11022229 |
| vitamin a mediated limb deformities in the common indian toad, bufo melanostictus (schneider). | several types of limb deformities were induced by vitamin a in b. melanostictus. these ranged from total suppression of all the limbs (ectromelia) to partial development of either the forelimb or the hindlimb or both (mesomelia) to reduction or absence of digits in either the forelimbs or hindlimbs or both and absence of long bones in either the forelimbs or hindlimbs or both (phocomelia) or duplication of the hindlimbs (polymelia). all the limb abnormalities were induced in the developing limbs ... | 2000 | 10927869 |
| a sleep inducing factor from common indian toad (bufo melanostictus, schneider) skin extract. | bufo melanostictus (common indian toad) acquire different bioactive substances in their skin during their life-time in wide ecological habitat. earlier investigation from this laboratory revealed that toad (b. melanostictus) skin extract (tse) posses different bioactive compounds of different diversity (das, m., auddy, b. and gomes, a., 1996. pharmacological study of the toad skin extract on experimental animals. indian j. pharmacol. 28, 72-76). among these sleep induction and sleep potentiation ... | 2000 | 10736480 |
| bidder's organ extract induced anaphylaxis in experimental animals. | bidder's organ (bo, a vestigeal organ), present in toad bufo melanostictus (schenider), is a characteristic feature of all male bufo. its possible anaphylactic properties are investigated on experimental animals. bo extract produced both in vivo and in vitro anaphylactic reaction in guineapig. dyspnoea and bronchoconstriction was a major cause of anaphylactic death. blood histamine level was significantly increased in the anaphylactic animals. bo extract significantly released histamine from cho ... | 2000 | 11218835 |
| isolation, purification and partial chemical characterization of a lethal factor from common indian toad (bufo melanostictus, schneider) skin extract. | indian toad (bufo melanostictus, schneider) skin extract (tse) is pharmacologically potent and probably contains several bioactive compounds [das et. al., indian j pharmacol, 28 (1996) 72]. a lethal factor was isolated and purified by neutral alumina column chromatography followed by hplc. spectroscopic (uv, ir, fab-mass) study indicated that the lethal factor (tse-lf) was a 254 da long chain compound with carbonyl, hydroxyl and ester as functional groups. ld50 of tse-lf was found to be 3.5 mg/k ... | 2001 | 12018580 |
| homeotic regeneration of eye in amphibian tadpoles and its enhancement by vitamin a. | after removal of both the lateral eyes of external gill stage tadpoles of the toad bufo melanostictus, the pineal organ gets transformed into a median eye. this type of transformation occurs in tadpoles of both control and vitamin a treated groups. however, vitamin a increases the likelihood of homeotic regeneration (57% in the control group and 71% in the vitamin a treated group). histological studies showed that the newly transformed median eye developed from the pineal organ. the pineal eye s ... | 2001 | 11807288 |
| nux vomica 30 prepared with and without succession shows antialcoholic effect on toads and distinctive molecular association. | adult toads, bufo melanostictus, were administered nux vomica (nux v) 30 prepared with and without succussion on the tongue. the drug was mixed with sterile distilled water at the rate 0.05ml/ml water and given orally 0.05ml/individual. the control consisted of blank ethanol solution. seeds of strychnos nuxvomica were ground and extracted with 90% ethanol in the laboratory. nux v 30 was prepared by successive dilution and succussion in 30 steps, nux v 30 u was prepared by successive dilution onl ... | 2001 | 11341461 |
| effect of thyroxine on some digestive enzymes of the adult male toad, bufo melanostictus. | thyroxine is known to play an important role during the developmental process of amphibians. the present work is designed on the hypothesis that a functional relationship exists between the thyroid gland and digestive physiology in the adult toad. three doses of thyroxine (25 microg/100 g; 50 microg/100 g; 100 microg/100 microg body weight) were orally administered daily to the adult male toad, bufo melanostictus and changes in -amylase, pepsin, trypsin, and esterase activities of the different ... | 2002 | 12597539 |
| phosphoglucose isomerases of hagfish, zebrafish, gray mullet, toad, and snake, with reference to the evolution of the genes in vertebrates. | phosphoglucose isomerase (pgi) is a protein with multiple functions. to infer its structure changes and evolution in vertebrates, we cloned cdnas encoding pgi genes from hagfish (paramyxine yangi), gray mullet (mugil cephalus), zebrafish (danio rerio), toad (bufo melanosticus), and snake (boiga kraepelini). only one pgi gene was cloned in each of hagfish, toad, and snake, but two pgi genes were found in zebrafish and gray mullet, respectively. the pgi of hagfish encodes 554 amino acids, in contr ... | 2002 | 11919278 |
| effect of whole body gamma radiation on hepatic ldh activity, lactate, pyruvate concentration and rate of oxygen consumption in bufo melanostictus. | whole body co60 gamma radiation induced changes in lactic dehydrogenase (ldh) activity, pyruvate, lactate content and rate of oxygen (o2) consumption in a tropical hibernating anuran (bufo melanostictus). in 3.5 and 7 gy treated groups, a significant increase in ldh activity and lactate/pyruvate ratio was observed, whereas a significant decrease in o2 consumption rate was observed in treated animals on post-irradiation day (pid) 1, 5 and 10. increase in ldh activity was observed on pid-1 in both ... | 2002 | 13677637 |
| an appraisal of post-irradiation metabolic changes in toad liver (bufo melanostictus). | whole body 60co gamma irradiation of b . melanostictus with two sublethal doses of 3.5 and 7 gy resulted in a significant increase (p < 0.001) in total lipid (hyperlipidemia), cholesterol content (hypercholesterolemia) and total free amino acid content, and significant decrease (p < 0.001) in total protein content in the liver tissues on post-irradiation day (pid) 1, 5 and 10 as compared to controls. the ratio of total lipid to cholesterol showed a gradual declining trend by pid-10 in the treate ... | 2002 | 12693706 |
| putative l-triiodothyronine receptors in the liver nuclei of mature tropical toad, bufo melanostictus. | thyroid hormones exert a major role in growth and differentiation of almost all types of tissues in animals, particularly in amphibian metamorphosis, through its specific nuclear receptor activation followed by gene expression. however, its function in mature tropical amphibians is less studied. the present study revealed the existence of a single class of specific nuclear receptor(s) in the liver nuclei of mature tropical toad, bufo melanostictus, with a dissociation constant of (3.7 +/- 0.9) x ... | 2004 | 15018064 |
| quality evaluation of traditional chinese drug toad venom from different origins through a simultaneous determination of bufogenins and indole alkaloids by hplc. | a simple and efficient hplc method has been developed to evaluate the quality of traditional chinese medicine toad venoms. the major bioactive ingredients, including 10 bufogenins and 4 indole alkaloids in the drug, were separated and quantified on a phenyl-hexyl column with a uv detector. a total of 27 toad venom samples from two species, bufo bufo gargarizans cantor and bufo melanostictus schneider, from the different drug production regions of china, were analyzed. the chromatograms showed si ... | 2005 | 16327195 |
| the "third eye"-- a new concept of trans-differentiation of pineal gland into median eye in amphibian tadpoles of bufo melanostictus. | median third eye was found to develop from transplanted pineal gland of external gill stage tadpoles in the recipient 5 toe stage tadpoles of bufo melanostictus. pineal gland along with a bit part of brain tissue of the donor external gill stage tadpole was cut out and transplanted into a pit made between two lateral eyes of 5 toe stage recipient tadpoles. half of the operated tadpoles were treated with vitamin a (15 iu/ml.) for 15 days. median "third eye" was found to develop in the both untrea ... | 2005 | 16121707 |
| composition, diversity, and spatial relationships of anurans following wetland restoration in a managed tropical forest. | we investigated the composition, diversity, and patterns of spatial use of an anuran community following a wetland restoration project in a managed tropical monsoon forest in southern taiwan. anurans comprising eight species in seven genera and four families reached a mean density of 0.025+/-0.004 anurans m(-2) within a year. the three most important species in terms of frequency of occurrence and relative abundance all had an early appearance; only the most abundant fejervarya limnocharis (62.2 ... | 2006 | 17116991 |
| antiproliferative, cytotoxic and apoptogenic activity of indian toad (bufo melanostictus, schneider) skin extract on u937 and k562 cells. | the antiproliferative, cytotoxic and apoptogenic activities of bufo melanostictus (indian common toad) skin extract (tse) on u937 and k562 leukemic cell line has been investigated. tse significantly (p<0.001) reduced the time-dependent cell proliferation and decreased mtt values in u937 and k562 cells. tse (ic50 doses) suppressed the proliferating cell nuclear antigen expression in both the cells. it was demonstrated that, tse (ic50 doses) primarily arrested the u937 and k562 cells at g1 phase o ... | 2006 | 16889807 |
| temperature and humidity alter prolactin receptor expression in the skin of toad (bufo bankorensis and bufo melanostictus). | bufo bankorensis and bufo melanostictus, the only two species of bufonidae genus in taiwan, live in habitats that differ in altitude and humidity. this study tested the hypothesis that prolactin receptor (prlr) expression responds to environmental change. western blot analysis showed that the prlr protein was widely distributed in brain, lung, liver, kidney, dorsal skin and ventral skin of toads. the level prlr protein was elevated in the dorsal skin of the two toad species treated with dry or w ... | 2006 | 17049288 |
| a new species of paracapillaria (nematoda: capillariidae) from the intestine of the toad duttaphrynus melanostictus (anura) from the malayan peninsula. | a new species of parasitic nematode, paracapillaria malayensis n. sp. (capillariidae), is described from the small intestine of the toad duttaphrynus melanostictus imported from the malayan peninsula to the czech republic. the new species differs from the only other congeneric species, paracapillaria spratti, mainly in the shape and structure of the spicular proximal end (with a lobular rim), smaller eggs (45-51 x 21-24 microm), longer spicule (336 microm), and the number (37-38) of stichocytes ... | 2007 | 17918374 |
| a crystalline compound (bm-anf1) from the indian toad (bufo melanostictus, schneider) skin extract, induced antiproliferation and apoptosis in leukemic and hepatoma cell line involving cell cycle proteins. | in our earlier communication, it was reported that indian toad (bufo melanostictus) skin extract (tse) possesses antiproliferative and apoptogenic activity in u937 and k562 cells [giri et al., 2006. antiproliferative, cytotoxic and apoptogenic activity of indian toad (bufo melanostictus, schneider) skin extract in u937 and k562 cells. toxicon 48 (4), 388-400]. in the present study, a compound (bm-anf1) has been isolated from the tse by alumina gel column chromatography, crystallized and evaluate ... | 2007 | 17692879 |
| toad poisoning in laos. | we describe two patients who developed severe illness after eating the skin and eggs of a toad, probably bufo melanostictus schneider, in southeastern laos. one boy died, and one developed a digoxin toxicity-like syndrome with bradycardia and heart failure but survived. a telephone survey of 16 lao provincial hospitals suggested that toad poisoning occurs in at least six provinces. that 93% of villagers in three villages in southeastern laos were aware that toads are poisonous but that 51% had e ... | 2007 | 17984341 |
| ubiquitous lens alpha-, beta-, and gamma-crystallins accumulate in anuran cornea as corneal crystallins. | corneal epithelium is known to have high levels of some metabolic enzymes such as aldehyde dehydrogenase in mammals, gelsolin in zebrafish, and alpha-enolase in several species. analogous to lens crystallins, these enzymes and proteins are referred to as corneal crystallins, although their precise function is not established in any species. although it is known that after lentectomy, the outer cornea undergoes transdifferentiation to regenerate a lens only in anuran amphibians, major proteins ex ... | 2007 | 17452334 |
| cryptosporidia: epicellular parasites embraced by the host cell membrane. | the ultrastructure of two gastric cryptosporidia, cryptosporidium muris from experimentally infected rodents (mastomys natalensis) and cryptosporidium sp. 'toad' from naturally infected toads (duttaphrynus melanostictus), was studied using electron microscopy. observations presented herein allowed us to map ultrastructural aspects of the cryptosporidian invasion process and the origin of a parasitophorous sac. invading parasites attach to the host cell, followed by gradual envelopment, with the ... | 2008 | 18158154 |
| new and already known acanthocephalans from amphibians and reptiles in vietnam, with keys to species of pseudoacanthocephalus petrochenko, 1956 (echinorhynchidae) and sphaerechinorhynchus johnston and deland, 1929 (plagiorhynchidae). | adults of 2 new species in 2 orders of acanthocephalans obtained from the intestines of terrestrial amphibians and reptiles collected between 1998 and 2004 in vietnam are described here. pseudoacanthocephalus nguyenthileae n. sp. (palaeacnthocephala: echinorhynchidae) was collected from 5 species of terrestrial amphibians: (1) the common sunda toad bufo melanostictus schneider (bufonidae); (2) paa verucospinosa (bourret); (3) gunther's amoy frog rana guentheri boulenger; (4) taipei frog r. taipe ... | 2008 | 18372639 |
| effects of diazinon on larvae of the asian common toad (bufo melanostictus, schneider 1799). | continuous subchronic exposure experiments were conducted to assess the effects of diazinon, an organophosphate pesticide, on the survival, growth and activity of larvae of the asian common toad bufo melanostictus. two larval stages, the gill stage (gosner stages 21 and 22) and gill-atrophy stage (gosner stages 24 and 25), were continuously exposed to 4 microg/l, 400 mirog/l, and 10 mg/l of commercial-grade diazinon for 30 d. treatments and untreated controls were maintained in triplicate with w ... | 2008 | 18611080 |
| new species of cryptosporidium tyzzer, 1907 (apicomplexa) from amphibian host: morphology, biology and phylogeny. | cryptosporidium fragile sp. n. (apicomplexa) is described from black-spined toads, duttaphrynus melanostictus (schneider) (amphibia, anura, bufonidae) from the malay peninsula. the parasitized animals were directly imported from malaysia and harboured c. fragile at the time of arrival. oocysts were subspherical to elliptical with irregular contour in optical section, measuring 6.2 (5.5-7.0) x 5.5 (5.0-6.5) microm. oocyst wall was smooth and colourless in light microscopy. the endogenous developm ... | 2008 | 18666410 |
| a survey on the faunal diversity of savar upazila, dhaka, bangladesh. | a survey was conducted during january to december 2006 to assess the status of faunal diversity of savar upazila, dhaka, bangladesh. a total of 30 species of birds, 24 species of winter birds, 7 species of reptiles, 3 species of amphibians, 15 species of mammalians and 32 species of fishes were recorded. relative abundance of those species were determined. of the birds, house sparrow (passer domesticus) was abundant while blyth's kingfisher (alcedo hercules), rock eagle owl (bubo bengalensis), h ... | 2008 | 18817158 |
| on the parasitic fauna of two species of anurans collected from sungai pinang, penang island, malaysia. | a total of fifty anurans, comprising of rana limnocharis and bufo melanostictus were collected from sungai pinang, balik pulau, penang. the prevalence, mean intensity and distribution of parasite species along the digestive tract were reported. seven species of parasites were recorded. blood parasites recovered were trypansomes and microfilariae. | 2008 | 18948888 |
| acute toxicity of synthetic pyrethroid cypermethrin to some freshwater organisms. | ninety-six hours static bioassays were made in the laboratory to determine acute toxicity of cypermethrin to five non-target freshwater organisms belonging to different taxa and niche. susceptibility of the organisms to cypermethrin was in the order: the crustacean diaptomus forbesi > the aquatic insect ranatra filiformis > the freshwater carp cyprinus carpio > the tadpole larva of the toad bufo melanostictus > the oligochaet worm branchiura sowerbyi. ninety-six hours lc50 values of aqueous cype ... | 2008 | 18058051 |
| checklist and simple identification key for frogs and toads from district iv of the mada scheme, kedah, malaysia. | a survey was conducted to catalogue the diversity of anurans in district iv of the muda agriculture development authority scheme (mada) in kedah darul aman, malaysia, from july 1996 to january 1997. eight species of anurans from three families were present in the study area. of these, the common grass frog (fejevarya limnocharis) was the most abundant, followed by mangrove frog (fejevarya cancrivora), long-legged frog (hylarana macrodactyla), and common toad (duttaphrynus melanostictus). puddle ... | 2009 | 24575178 |
| curcumin synergizes the growth inhibitory properties of indian toad (bufo melanostictus schneider) skin-derived factor (bm-anf1) in hct-116 colon cancer cells. | curcumin, an active ingredient of turmeric with no discernable toxicity, inhibits the growth of transformed cells and the development and progression of colon carcinogenesis in experimental animals. recent data from one of our laboratories demonstrated that a crude skin extract or a purified crystalline compound (bufo melanostictus-antineoplastic factor 1, bm-anf1) from indian common toad (bufo melanostictus, schneider) skin inhibits the growth of human leukemic cells. the present investigation ... | 2009 | 19331178 |
| association preference and mechanism of kin recognition in tadpoles of the toad bufo melanostictus. | in experiments with specially designed choice tanks, tadpoles of bufo melanostictus spend significantly greater amounts of time near kin than near non-kin. however, in the absence of kin members, they prefer to spend more time near non-kin rather than stay away in isolation in the opposite blank zone with no company. this implies that association of toad tadpoles with their kin is due to attraction rather than repulsion from non-kin. experiments designed to elucidate the sensory basis of kin rec ... | 2009 | 19805905 |
| developmental disruptions induced by insect growth regulator (novaluron) in bufo melanostictus tadpoles. | novaluron is an insect growth regulator (igr) used against fruit-borers and domestic pests. in this study, effects of different concentrations (0.5, 0.75, 1.0, 1.5 microg l(-1)) of novaluron on the tail regeneration, limb development and metamorphosis were examined in bufo melanostictus. thyroxine (1.0, 2.0, 3.5, 5.0 microg l(-1)), which promotes amphibian development/metamorphosis, and vitamin a (5, 20, 40, 60 iu l(-1)), which disrupts development and induce polymelia, were used for comparison. ... | 2010 | 20186694 |
| rapid structural identification of cytotoxic bufadienolide sulfates in toad venom from bufo melanosticus by lc-dad-ms(n) and lc-spe-nmr. | toad venom, namely, "chansu" in china, has been widely used for the treatment of heart failure, sores, pains, and various cancers. upon lc-ms analysis of the venom from bufo melanosticus collected in indonesia, new bufadienolide sulfates were identified. for a complete characterization, the meoh extract of the toad venom from b. melanosticus was fractionated by preparative hplc, and the structures of five new buadienolide sulfates (1-5) along with one new bufogenin (6) were rapidly elucidated on ... | 2010 | 20361780 |
| comparison of toad venoms from different bufo species by hplc and lc-dad-ms/ms. | toad venom, called chansu in china, has been widely used for the treatment of heart failure, sores, pains, and various cancers for a long time in clinic. | 2010 | 20637273 |
| chlorpyrifos-induced toxicity in duttaphrynus melanostictus (schneider 1799) larvae. | this study investigates the effects of continuous exposure to a widely used organophosphate pesticide, chlorpyrifos, on survival, growth, development, and activity of larvae of the asian common toad, duttaphrynus melanostictus schneider 1799. larvae were continuously exposed to six different concentrations (1-1,500 mug l(-1)) of commercial-grade chlorpyrifos for 14 days and monitored for 1 additional week. chlorpyrifos at >/=1,000 mug l(-1) caused significantly high and dose-dependent mortality, ... | 2010 | 20669016 |
| apoptogenic activity and toxicity studies of a cytotoxic protein (bmp1) from the aqueous extract of common indian toad (bufo melanostictus schneider) skin. | a protein (bmp1) was purified from common indian toad (bufo melanostictus, schneider) skin through deae cellulose ion exchange chromatography and high performance liquid chromatography. the molecular weight of the bmp1 was found to be 79 kda. bmp1 (0.5 and 1 mg/kg/day, i.p.) significantly decreased the number of viable ehrlich ascites carcinoma (eac) cells, thereby increased the lifespan of eac bearing mice (p<0.001). mtt values reduced significantly with the treatment of bmp1 (0.5 and 1.0 mg/kg ... | 2010 | 21144856 |
| helminth parasite communities in anuran amphibians of kalesar wildlife sanctuary (haryana), india. | helminth parasite fauna in anuran amphibia were investigated during the general faunistic surveys of kalesar wildlife sanctuary, situated in haryana state. three species of amphibian hosts were found to harbour 12 genera of helminth parasites. the prevalence, intensity and abundance were studied. euphlyctis cyanophlyctis harboured maximum parasite species followed by fejervarya limnocharis and duttaphrynus melanostictus. in e. cyanophlyctis, among nematode parasites, the genus camallanus was mos ... | 2010 | 21966125 |
| ecotoxicological assessment of cobalt used as supplement in the diet of common carp cyprinus carpio. | experiments were performed in the laboratory to determine if excess levels of co used as dietary supplement (0.0, 0.05, 0.10 and 1.0%) to enhance growth of the fish cyprinus carpio was safe for aquatic organisms. lethal concentrations of co for tadpole of toad bufo melanostictus (96 h lc(50), 17.2 mg/l), oligochaet worm branchiura sowerbyi (96 h lc(50), 179 mg/l) and crustacean zooplankton diaptomus forbesi (96 h lc(50), 1.5 mg/l) were compared with the concentration of co in the medium leached ... | 2011 | 21858707 |
| effect and mechanism of action of resveratrol: a novel melanolytic compound from the peanut skin of arachis hypogaea. | the present work was carried out to determine the effects of ethanolic extracts of arachis hypogaea and its active ingredient resveratrol on the isolated tail melanophores of the bufo melanostictus to find the mechanism of skin lightening at the cellular level. | 2011 | 21929291 |
| anticancer activity of a low immunogenic protein toxin (bmp1) from indian toad (bufo melanostictus, schneider) skin extract. | earlier, a protein (bmp1, mw-79kda) had been isolated from indian toad (bufo melanostictus) skin aqueous extract possessed anticancer activity against eac bearing mice (bhattacharjee et al., 2011). in the present study, the anti-proliferative and apoptogenic activities of bmp1 have been evaluated in leukemic (u937 and k562) and hepatoma (hepg2) cells. bmp1 dose dependently inhibited u937 and k562 cell growth having ic(50) values of 49 μg/ml and 30 μg/ml respectively. the anti-proliferative activ ... | 2011 | 21635912 |
| the herpetofauna of timor-leste: a first report. | fieldwork conducted throughout timor-leste in september 2004 and july 2009 resulted in a collection or recording of 263 herpetological specimens (100 amphibians, 163 reptiles), comprising at least seven species of frogs and toads, 20 species of lizards, seven species of snakes, two species of turtles, and one species of crocodile. among the amphibians, the most frequently encountered species were toads (duttaphrynus melanostictus), rice paddy frogs (genus fejervarya), and rhacophorid treefrogs ( ... | 2011 | 21852932 |
| purification and characterization of albumin from frog skin of duttaphrynus melanostictus. | following determination of trypsin inhibitory activity, a serine protease inhibitor was purified and characterized from frog duttaphrynus melanostictus serum. it was identified as serum albumin, with molecular weight of 67 kda (dma-serum). different from bovine serum albumin, dma-serum potently inhibited trypsin with similar k(i) values around 1.6 × 10⁻⁷ m. no inhibitory effect on thrombin, chymotrypsin, elastase and subtilisin was observed under the assay conditions. the n-terminal amino acid i ... | 2011 | 21858423 |
| effect of an increase in environmental temperature on testicular androgenesis and spermatogenesis in toad (bufo melanostictus) during hibernating season. | activities of key testicular androgenic enzymes [δ(5), 3β-hydroxysteroid dehydrogenase (δ(5), 3β-hsd) and 17β-hydroxysteroid dehydrogenase (17β-hsd)], plasma levels of testosterone, and testicular gametogenic activities were studied in heat-exposed adult male toads during hibernating season for two consecutive years. exposure of toads to an elevated environmental temperature for 14 and 21 days resulted in significant elevation of testicular δ(5), 3β-hsd and 17β-hsd activities, along with plasma ... | 2011 | 22147595 |
| psoralea corylifolia extracts stimulate cholinergic-like psoralen receptors of tadpole-tail melanophores, leading to skin darkening. | the present work was carried out to determine the effects of lyophilized seed extracts of psoralea corylifolia along with pure psoralen, its active ingredient on the isolated tail-piece melanophores of bufo melanostictus, a type of disguised smooth muscle cells, which offer excellent in vitro opportunities for studying the effects of pharmacological and pharmaceutical agents. in the present study, it was found that lyophilized extract of p. corylifolia and its active ingredient psoralen induced ... | 2011 | 20863173 |
| deriving freshwater quality criteria for iron, lead, nickel, and zinc for protection of aquatic life in malaysia. | freshwater quality criteria for iron (fe), lead (pb), nickel (ni), and zinc (zn) were developed with particular reference to aquatic biota in malaysia, and based on usepa's guidelines. acute toxicity tests were performed on eight different freshwater domestic species in malaysia which were macrobrachium lanchesteri (prawn), two fish: poecilia reticulata and rasbora sumatrana, melanoides tuberculata (snail), stenocypris major (ostracod), chironomus javanus (midge larvae), nais elinguis (annelid), ... | 2012 | 22919358 |
| cadmium-induced toxicity on larvae of the common asian toad duttaphrynus melanostictus (schneider 1799): evidence from empirical trials. | this paper investigates the toxicity of cadmium (cd) on young stages of the common asian toad duttaphrynus melanostictus (schneider 1799). signs of acute toxicity were evident in tadpoles repeatedly exposed to five concentrations ranging from 0.002 to 2 mg l(-1)of cd which included environmentally relevant levels. mortality at concentrations of 0.02 mg l(-1) and above was enhanced from 2 % at 0.02 mg l(-1) to 100 % at 1 mg l(-1), in a dose-dependent manner. significant growth impairment was evid ... | 2012 | 22526988 |
| immunohistochemical localization of cathepsin d and a possible role of melanocytes during tail resorption in tadpoles of a tropical toad. | programmed cell death during anuran tail resorption is primarily brought about by apoptosis. cathepsin d, a lysosomal aspartyl protease, is involved in the death of tail tissues. thus, anuran tail resorption presents an ideal model to study cathepsin-mediated cell death during vertebrate development. present study describes the trend of specific activity of cathepsin d in the tail of different developmental stages and immunohistochemical localization of cathepsin d in the tail tissues of the com ... | 2012 | 22505219 |
| on the novel action of melanolysis by a leaf extract of aloe vera and its active ingredient aloin, potent skin depigmenting agents. | the present study was carried out to investigate the effects of an aloe vera leaf extract, along with its standard active ingredient aloin, on the isolated tail melanophores of bufo melanostictus tadpoles, which are a type of disguised smooth muscle cells offering excellent in vitro opportunities for studying the effects of pharmacological and pharmaceutical agents. it was found that the leaf extract of a. vera and its active ingredient aloin induced powerful, dose-dependent, physiologically sig ... | 2012 | 22495441 |
| prospective randomised evaluation of traditional chinese medicine combined with chemotherapy: a randomised phase ii study of wild toad extract plus gemcitabine in patients with advanced pancreatic adenocarcinomas. | an intravenous formulated extract of the venom of the wild toad bufo bufo gargarizans cantor or bufo melanostictus schneider, huachansu, is currently used in china for the treatment of lung, liver, pancreatic, and colorectal cancers. we performed a randomised, single-blinded, phase ii clinical study of huachansu plus gemcitabine versus placebo plus gemcitabine in patients with locally advanced and/or metastatic pancreatic adenocarcinomas. | 2012 | 22782343 |
| malformations and mortality in the asian common toad induced by exposure to pleurolophocercous cercariae (trematoda: cryptogonimidae). | malformations and increased mortality due to infection by the digenetic trematode, riberioa ondatrae have been reported for many species of amphibians. severe malformations have also been reported in the common hourglass tree frog, polypedates cruciger induced by pleurolophocercous cercariae in sri lanka in addition to the changes in the behaviour, development and survival of the host. we exposed pre-limb bud stage tadpoles (gosner stages 25-26) of the asian common toad, duttaphrynus melanostict ... | 2013 | 23353759 |
| efficacy and safety of huachansu combined with chemotherapy in advanced gastric cancer: a meta-analysis. | huachansu, a chinese medicine that comes from dried toad venom from the skin glands of bufo bufo gargarizans cantor or bufo melanostictus schneider, has been used in treatment of cancers. we conducted a meta-analysis to evaluate the efficacy and safety of huachansu combined with chemotherapy for advanced gastric cancer. | 2013 | 23692970 |
| molecular cloning and characterization of cystatin, a cysteine protease inhibitor, from bufo melanostictus. | cystatins are efficient inhibitors of papain-like cysteine proteinases, and they serve various important physiological functions. in this study, a novel cystatin, cystatin-x, was cloned from a cdna library of the skin of bufo melanostictus. the single nonglycosylated polypeptide chain of cystatin-x consisted of 102 amino acid residues, including seven cysteines. evolutionary analysis indicated that cystatin-x can be grouped with family 1 cystatins. it contains cystatin-conserved motifs known to ... | 2013 | 24096668 |
| quality assessment of serially ultradiluted and agitated drug digitalis purpurea by emission spectroscopy and clinical analysis of its effect on the heart rate of indian bufo melanostictus. | the investigation of ultradiluted (homeopathic) drugs is extremely interesting and challenging, and from that point of view this study shows novelty. a study of in vivo changes in heart rate of the indian bufo melanostictus caused by commercially available serially ultra-diluted and agitated extract of digitalis purpurea has been tried in order to understand their pharmacological role. rr interval (of ecg) was compared after intraperitoneal administration of serially diluted and agitated digital ... | 2013 | 26555986 |
| a new species of duttaphrynus (anura:bufonidae) from northeast india. | a new species of montane toad duttaphrynus is described from nagaland state of northeast india. the new species is diagnosable based on following combination of characters: absence of preorbital, postorbital and orbitotympanic ridges, elongated and broad parotid gland, first finger longer than second and presence of a mid-dorsal line. the tympanum is hidden under a skin fold (in male) or absent (in female). the species is compared with its congers from india and indo-china. we propose to conside ... | 2013 | 26213769 |
| sensitivity of toad tadpoles, bufo melanostictus (schneider), to heavy metals. | 2013 | 3814877 | |
| protozoan parasites of four species of wild anurans from a local zoo in malaysia. | the parasitic protozoan fauna in sixty-six anurans comprising of duttaphrynus melanostictus, phrynoidis juxtaspera, hylarana erythraea and polypedates leucomystax collected from zoo negara malaysia was investigated. the distribution and prevalence rate of parasitic species in the digestive tract and blood were examined. seven species of intestinal protozoa (opalina ranarum, cepedea dimidiata, nycthetorus cordiformis, entamoeba ranarum, iodamoeba butschlii, endamoeba blattae, and tritrichomonas s ... | 2013 | 24522131 |
| [prey selection by tiger frog larvae (hoplobatrachus chinensis) of two sympatric anuran species' tadpoles]. | we examined the prey selection and behavioral responses of tiger frog hoplobatrachus chinensis larvae exposed to unpalatable and palatable sympatric prey tadpoles, bufo melanostictus and pelophylax nigromaculatus. we found that after a short exposure to the toxic toad tadpoles b. melanostictus, predators may learn to decrease going after unpalatable prey, subsequently it seems they may express short-term behavioral memory in order to avoid the toxic prey. in general, h. chinensis showed no prefe ... | 2013 | 23775997 |
| a comparative study of hepatic mitochondrial oxygen consumption in four vertebrates by using clark-type electrode. | the present study was undertaken to establish a comparative account on hepatic mitochondrial oxygen consumption of clarias gariepinus (fish), bufo melanostictus (amphibian), gallus gallus (bird) and rattus norvegicus (mammal) and to correlate it with their specific metabolic rate (smr). mitochondrial oxygen consumption was measured with a clarke-type electrode with succinate and pyruvate/malate as substrates. adp was used to start state-iii respiration. the results show that rats and chickens ha ... | 2013 | 23739884 |
| effect of cold exposure on testicular delta 5-3 beta and 17 beta hydroxysteroid dehydrogenase activities and plasma levels of testosterone in toad (bufo melanostictus) in breeding and hibernating season: duration-dependent response. | the purpose of this study was to show the effect of cold exposure on testicular activities in breeding and hibernating seasons in the toad. adult male toads were placed in a cold chamber in both breeding and hibernating seasons for periods of 7, 14 and 21 days. at the time of sacrifice on the 15th and 22nd days, cold-exposed animals showed a decrease in testicular weight, testicular delta 5-3 beta and 17 beta-hydroxysteroid dehydrogenase activities and low levels of plasma testosterone both in b ... | 2013 | 9629432 |
| effect of lithium chloride on spermatogenesis, testicular delta 5- 3 beta- and 17 beta-hydroxysteroid dehydrogenase activities in toad (bufo melanostictus). | the purpose of the present study was to show the effects of lithium on testicular activities in the toad. adult male toads were injected with lithium chloride (200 micrograms/toad) of alternate days for 21 days. at the moment of sacrifice on 22nd day, lithium treated animal showed decreases in testicular weight and leydig cell nuclear area along with inhibition of spermatogenesis and testicular delta 5-3 beta as well as 17 beta-hydroxysteroid dehydrogenase activities. the results of our present ... | 2013 | 2549814 |
| [comparison of the chemical composition between fresh and dried venenum bufonis by uplc-orbitrap ms]. | to identify the active components in bufo melanostictus schneider and clarify the difference between fresh and dried venenum bufonis, a uplc-orbitrap ms method has been established. the separation was performed with gradient elution of acetonitrile and water (with 0.1% formic acid) as mobile phase. by comparing their retention time and high resolution mass data of venenum bufonis extracts, 39 effective components were primarily identified by ms/ms analysis in positive ion mode. twenty-six of the ... | 2014 | 25577877 |
| berberine-induced pigment dispersion in bufo melanostictus melanophores by stimulation of beta-2 adrenergic receptors. | reduced production of melanin by decreased or the absence of melanocytes leads to various hypopigmentation disorders, and the development of melanogenetic agents for photoprotection and hypopigmentation disorders is one of the top priority areas of research. hence, the present study was carried out to elucidate the ability of berberine, a principal active ingredient present in the roots of the herb berberis vulgaris to stimulate pigment dispersion in the isolated skin melanophores of the toad bu ... | 2014 | 24099619 |
| study on anti-osteosarcoma activity of ethanol extract of venenum bufonis in vitro. | venenum bufonis is the dried white secretion of the auricular and skin glands of bufo gargarizans cantor, or bufo melanostictus schneider, bufonidae. it is used in the treatment of deep-rooted carbuncle, boils and swelling; pain in the throat, heart stroke, coma, abdominal pain, vomiting and diarrhea. the objective of this paper is to preliminarily observe the effects of ethanol extract of venenum bufonis on growth, and proliferation of human osteosarcoma u2os cell lines, and to provide a theore ... | 2014 | 24653556 |
| on a new species of aplectana (nematoda, cosmocercidae) from kulti, burdwan, west bengal, india. | during routine survey for amphibian nematodes from coalfield areas of west bengal, india 7 male and 12 female nematodes were recovered from the rectum of one out of three duttaphrynus melanostictus (schneider, 1899) examined for helminth infection from kulti, burdwan. on examination the recovered nematodes were found to belong to a new species of the genus aplectana (nematoda: cosmocercidae) and the name aplectana duttaphryni sp. nov. is proposed for them. this species is most similar to those s ... | 2014 | 25236282 |
| adrenal cortex in stress and hibernation--a histochemical study in toad (bufo melanostictus). | 2014 | 4186700 | |
| consequences of physical disturbance by tadpoles and snails on chironomid larvae. | indirect interactions among community members impact on organisms. the effects of two snails, banded pond snail, bellamya bengalensis (lamarck), and red-rimmed melania, melanoides tuberculata (müller), and tadpoles of asian common toad, duttaphrynus melanostictus (schneider), on nonbiting midge larvae, chironomus striatipennis kieffer, were observed in experimental microcosm. decrease in tube number and tube length of midge larvae was observed compared to control condition due to introduction of ... | 2014 | 24672384 |
| temperature-dependent acute toxicity of methomyl pesticide on larvae of 3 asian amphibian species. | relative to other animal taxa, ecotoxicological studies on amphibians are scarce, even though amphibians are experiencing global declines and pollution has been identified as an important threat. agricultural lands provide important habitats for many amphibians, but often these lands are contaminated with pesticides. the authors determined the acute toxicity, in terms of 96-h median lethal concentrations, of the carbamate pesticide methomyl on larvae of 3 asian amphibian species, the asian commo ... | 2015 | 25959379 |