Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| sugar sensing and ca2+-calmodulin requirement in vitis vinifera cells producing anthocyanins. | we have previously reported that sucrose modulates anthocyanin biosynthesis in cell suspension cultures of vitis vinifera l. the main role of sugar in this response does not seem to be that of general carbohydrate source for the supply of energy. in the present work, a number of pharmacological agents were used to further investigate the components of the signal transduction pathway involved in the induction of anthocyanin biosynthesis by sugar. we found that the phosphorylation of hexose by hex ... | 2000 | 10746878 |
| determination of tryptophan and tryptophan metabolites in grape must and wine. | tryptophan (trp) and its metabolites, especially indole-3-acetic acid (iaa), are considered as potential precursors of 2-aminoacetophenone (aap), an aroma compound which causes the "untypical aging off-flavor" (uta) in vitis vinifera white wines. in this study rp-hplc with fluorescence detection was used for the qualitative and quantitative analysis of trp and trp-metabolites in 39 grapes, 22 grape musts and 16 wines, to which different viticultural conditions (ripeness, pruning, strip of leaves ... | 1999 | 10721117 |
| differential screening indicates a dramatic change in mrna profiles during grape berry ripening. cloning and characterization of cdnas encoding putative cell wall and stress response proteins. | we used differential screening to isolate ripening-associated cdnas from a shiraz grape (vitis vinifera l.) berry cdna library. a rapid increase in the mrna levels of a number of cdnas not present in unripe fruit occurred in grape berries at the onset of ripening. the putative translation products of some of these clones had homologs in other species that are involved in cell wall structure. these included four proline-rich proteins, a small protein that is similar to the non-catalytic, n-termin ... | 2000 | 10712544 |
| occurrence of resveratrol and pterostilbene in age-old darakchasava, an ayurvedic medicine from india. | 'darakchasava' is a well known indian herbal preparation of which the main ingredient is vitis vinifera l. this 'ayurvedic' medicine is prescribed as a cardiotonic and also given for other disorders. hplc analysis of this age old formulation revealed the presence of polyphenols like resveratrol and pterostilbene. these phenolic compounds are now known as antioxidants, cancer chemopreventive agents, and also known to reduce mortality from coronary heart disease by increasing high density lipoprot ... | 1999 | 10624864 |
| [leukocyanidines and collagenases: in vitro enzyme inhibition activity]. | the matrix metalloproteinases (mmps) play a key role in the extracellular matrix turnover. this protein family has been involved in some ocular pathologies such as glaucoma, diabetic retinopathy, macular degeneration, vitreous degeneration and corneal stroma ulceration cleaving all the matrix components. in the present study we evaluated the action of leucocyanidin from vitis vinifera seeds as non toxic inhibitor of these proteinases. | 2014 | 10605164 |
| phosphoenolpyruvate carboxykinase plays a role in interactions of carbon and nitrogen metabolism during grape seed development | phosphoenolpyruvate carboxykinase (pepck) was shown to be present in a range of developing seeds by measurement of its activity and by immunoblotting. its function was investigated during grape (vitis vinifera l.) seed development. the maximum abundance of pepck coincided with the deposition of storage reserves. at this stage of development, immunohistochemistry showed that pepck was very abundant in a layer of cells located at the boundary of developing storage tissues and in the chalaza (close ... | 1999 | 10592027 |
| indigenous yeasts associated with two vitis vinifera grape varieties cultured in southern spain. | the yeast microbiota present on the surface of grapes of two vitis vinifera varieties, pedro ximénez and tempranillo de rioja, grown in the montilla-moriles region of southern spain was identified. the changes between veraison and the physiological ripeness time during 3 years were monitored. overall, the yeast microbiota isolated was of oxidative metabolism. sporobolomyces roseus and cryptococcus albidus species occurred at all physiological stages, in the two vitis vinifera varieties, and the ... | 1999 | 10582378 |
| assessment of the native electrophoretic analysis of total grape must proteins for the characterization of vitis vinifera l. cultivars. | the findings of an ampelographic analysis of vines belonging to a germoplasm bank were compared to the results of native electrophoresis of the total proteins in their musts. cluster analysis of the data from the morphological description produced correct groupings, in terms of variety, for all samples. when cluster analysis was performed on the electrophoretic data, 10 of the 11 musts studied were grouped correctly. electrophoresis was also performed on 30 musts made from a mixture of grapes fr ... | 1999 | 10563858 |
| relation between fatty acid content and its evolution during fermentation and utilization of free amino acids in vacuum-filtered viura must. | the activity of proteins that transport amino acids changes depending on the unsaturated residue which is introduced into the phospholipids of saccharomycescerevisiae membrane. the aim of this study was to observe the influence of fatty acid content and its evolution during fermentation on the utilization of free amino acids in vitis vinifera var. viura must clarified by vacuum filtration. the results demonstrate that filtration of the must did not reduce the concentration of free amino acids, b ... | 1998 | 10554194 |
| changes in the phytoalexin content of various vitis spp. in response to ultraviolet c elicitation. | the phytoalexin production potential of three american vitis species and that of three cultivars of vitis vinifera were evaluated in response to uv-c irradiation. time course changes in resveratrol, piceid, epsilon-viniferin, and pterostilbene contents were studied within 3 days after a short uv-c irradiation. results show that the two major stilbenes accumulated as a response to uv-c elicitation are resveratrol and epsilon-viniferin, a resveratrol dehydrodimer, the concentration of both compoun ... | 1999 | 10552833 |
| effect of pesticide residues on the aromatic composition of red wines. | the influence of pesticide residues on the aromatic composition (major and minor volatiles) of red wines made from vitis vinifera (monastrell var.) was studied by comparing the concentration of aromas in wines made from grapes subjected (or not) to phytosanitary treatment with chlorpyrifos, fenarimol, mancozeb, metalaxyl, penconazole, and vinclozolin, according to the agricultural practice of the area. the analytical determination of the major volatiles was made by gas chromatography using a fla ... | 1999 | 10552572 |
| regional origin assignment of red wines from valencia (spain) by (2)h nmr and (13)c irms stable isotope analysis of fermentative ethanol. | the use of the stable hydrogen and carbon isotope ratios of fermentative ethanol as suitable environmental fingerprints for the regional origin identification of red wines from valencia (spain) has been explored. monovarietal vitis vinifera l. cvs. bobal, tempranillo, and monastrell wines have been investigated by (2)h nmr and (13)c irms for the natural ranges of site-specific (2)h/(1)h ratios and global delta(13)c values of ethanol over three vintage years. statistically significant interregion ... | 1999 | 10552539 |
| effects of copper exposure in tissue cultured vitis vinifera. | the present study determined the effects of copper treatment on some biochemical parameters in a closed system. sauvignon grapevines were cultured in agar and exposed to copper levels ranging from 0.07 to 10 microg cu/g medium. chlorophylls, carotenoids, lipids, sucrose, soluble sugars, starch, cellulose, and minerals in root, leaves, and sap were determined. copper levels over 5 microg cu/g inhibit root and plantlet development. copper-exposed plants have higher levels of chlorophylls and carot ... | 1999 | 10552520 |
| analysis of the relative increase in photosynthetic o(2) uptake when photosynthesis in grapevine leaves is inhibited following low night temperatures and/or water stress | we found similarities between the effects of low night temperatures (5 degrees c-10 degrees c) and slowly imposed water stress on photosynthesis in grapevine (vitis vinifera l.) leaves. exposure of plants growing outdoors to successive chilling nights caused light- and co(2)-saturated photosynthetic o(2) evolution to decline to zero within 5 d. plants recovered after four warm nights. these photosynthetic responses were confirmed in potted plants, even when roots were heated. the inhibitory effe ... | 1999 | 10517860 |
| the generation of active oxygen species differs in tobacco and grapevine mesophyll protoplasts. | our previous results have shown that oxidative stress may reduce the regeneration potential of protoplasts, but only protoplasts that are able to supply extracellularly h(2)o(2) can actually divide (c.i. siminis, a.k. kanellis, k.a. roubelakis-angelakis [1993] physiol plant 87: 263-270; c.i. siminis, a.k. kanellis, k.a. roubelakis-angelakis [1994] plant physiol 1105: 1375-1383; a. de marco, k.a. roubelakis-angelakis [1996a] plant physiol 110: 137-145; a. de marco, k.a. roubelakis-angelakis [1996 ... | 1999 | 10482675 |
| historical genetics: the parentage of chardonnay, gamay, and other wine grapes of northeastern france. | the origins of the classic european wine grapes (vitis vinifera) have been the subject of much speculation. in a search for parental relationships, microsatellite loci were analyzed in more than 300 grape cultivars. sixteen wine grapes that have long been grown in northeastern france, including 'chardonnay', 'gamay noir', 'aligoté', and 'melon', have microsatellite genotypes consistent with their being the progeny of a single pair of parents, 'pinot' and 'gouais blanc', both of which were widesp ... | 1999 | 10477519 |
| technical advance: confocal measurement of the three-dimensional size and shape of plant parenchyma cells in a developing fruit tissue. | parenchyma cells from the inner mesocarp of a grape berry (vitis vinifera l. cv. chardonnay) were visualised in three-dimensions within a whole mount of cleared, stained tissue using confocal laser scanning microscopy and digital image reconstruction. the whole berry was fixed, bisected longitudinally, cleared in methyl salicylate, stained with safranin o and mounted in methyl salicylate. optical slices were collected at 1.0 µm intervals to a depth of 150 µm. neighbouring z-series were joined po ... | 1999 | 10476070 |
| cloning and expression of a hexose transporter gene expressed during the ripening of grape berry. | the ripening of grape (vitis vinifera l.) is characterized by massive sugar import into the berries. the events triggering this process and the pathways of assimilate transport are still poorly known. a genomic clone vvht1 (vitis vinifera hexose transporter1) and the corresponding cdna encoding a hexose transporter whose expression is induced during berry ripening have been isolated. vvht1 is expressed mainly in the berries, with a first peak of expression at anthesis, and a second peak about 5 ... | 1999 | 10444092 |
| adult onset grape hypersensitivity causing life threatening anaphylaxis. | adverse reactions to foods are encountered much less frequently in adults than in the children. adult onset hypersensitivity to grapes has not been previously reported. | 1999 | 10437812 |
| cloning and expression of an arginine decarboxylase cdna from vitis vinifera l. cell-suspension cultures. | arginine decarboxylase (adc; ec 4.1.1.19) is a key enzyme in one of the two pathways to putrescine. we present the first adc cdna from a woody perennial plant species, the grapevine (vitis vinifera l.), which exhibits 70-80% homology with other dicot adcs. the effects of ammonium, nitrate, and putrescine on adc specific activity, soluble polyamine levels, adc-mrna, endogeneous arginine and ornithine, and arginase specific activity were investigated in suspension cultures of grapevine cells. the ... | 1999 | 10420650 |
| proline accumulation in developing grapevine fruit occurs independently of changes in the levels of delta1-pyrroline-5-carboxylate synthetase mrna or protein. | mature fruit of grapevine (vitis vinifera) contains unusually high levels of free proline (pro; up to 24 micromol or 2.8 mg/g fresh weight). pro accumulation does not occur uniformly throughout berry development but only during the last 4 to 6 weeks of ripening when both berry growth and net protein accumulation have ceased. in contrast, the steady-state levels of both the mrna encoding v. vinifera delta1-pyrroline-5-carboxylate synthetase (vvp5cs), a key regulatory enzyme in pro biosynthesis, a ... | 1999 | 10398729 |
| production of 13c-labelled anthocyanins by vitis vinifera cell suspension cultures. | the use of plant cell cultures for producing isotopically (13c) labelled phenolic substances is reported. vitis vinifera cells synthesize high levels of anthocyanins when they are cultured in a polyphenol synthesis-inducing medium. three major anthocyanin monoglucosides found in red wine were identified in grape cells: cyanidin-3-o-beta-glucoside, peonidin-3-o-beta-glucoside, and malvidin-3-o-beta-glucoside. kinetic study of the intracellular level of phenylalanine and its metabolites showed tha ... | 1999 | 10392469 |
| stilbene compounds: from the grapevine to wine. | stilbenes are natural compounds occurring in a number of plant families, including vitaceae and (within this family) vitis vinifera l., which is the most important species grown worldwide for grape and wine production. stilbenes (resveratrol and viniferins) are present in grapevine as constitutive compounds of the woody organs (roots, canes, stems) and as induced substances (in leaves and fruit) acting as phytoalexins in the mechanisms of grape resistance against certain pathogens. resveratrol ( ... | 1999 | 10370866 |
| [clinical and capillaroscopic evaluation of chronic uncomplicated venous insufficiency with procyanidins extracted from vitis vinifera]. | the pharmacological treatment of non-complicated chronic venous insufficiency is a current and well-debated topic. the introduction of new products with action on the venous system, improved knowledge on the physiopathology of venous insufficiency and the possibility provided by new analytical instruments, have given new impulse to the consolidation of the clinical value of phlebotonics in this indication. | 2013 | 10356940 |
| diet enriched with procyanidins enhances antioxidant activity and reduces myocardial post-ischaemic damage in rats. | aim of this work was to study the efficacy of procyanidins from vitis vinifera seeds, a standardized mixture of polyphenol antioxidants, on cardiac mechanics following ischemia/reperfusion stunning in the rat, after 3 weeks supplementation. young and aged male rats were fed a diet enriched with procyanidins complexed (1:3 w/w) with soybean lecithin (2.4%); control animals (ctr-young and ctr-aged) received an equal amount of lecithin and 2 additional groups of animals the standard diet. at the en ... | 1999 | 10069526 |
| purification and characterization of a nadph-dependent aldehyde reductase from mung bean that detoxifies eutypine, a toxin from eutypa lata1 | eutypine (4-hydroxy-3-[3-methyl-3-butene-1-ynyl] benzaldehyde) is a toxin produced by eutypa lata, the causal agent of eutypa dieback in the grapevine (vitis vinifera). eutypine is enzymatically converted by numerous plant tissues into eutypinol (4-hydroxy-3-[3-methyl-3-butene-1-ynyl] benzyl alcohol), a metabolite that is nontoxic to grapevine. we report a four-step procedure for the purification to apparent electrophoretic homogeneity of a eutypine-reducing enzyme (ere) from etiolated mung bean ... | 1999 | 9952458 |
| rhinoconjunctivitis and asthma caused by vine pollen: a case report. | the vine (vitis vinifera) is a cultivated plant that is found in some european and american countries. its pollen gathers in small quantities during a short pollination period in the months of may and june. allergy to vine pollen has not been previously documented. | 1999 | 9949317 |
| a novel nadph-dependent aldehyde reductase gene from vigna radiata confers resistance to the grapevine fungal toxin eutypine. | eutypine, 4-hydroxy-3-(3-methyl-3-butene-1-ynyl) benzyl aldehyde, is a toxin produced by eutypa lata, the causal agent of eutypa dieback of grapevines. it has previously been demonstrated that tolerance of some cultivars to this disease was correlated with their capacity to convert eutypine to the corresponding alcohol, eutypinol, which lacks phytotoxicity. we have thus purified to homogeneity a protein from vigna radiata that exhibited eutypine-reducing activity and have isolated the correspond ... | 1998 | 9881154 |
| red-wine polyphenols and inhibition of platelet aggregation: possible mechanisms, and potential use in health promotion and disease prevention. | an extract from red-wine grape fermentation, anox has been developed as a source of red-wine polyphenols, which are thought to inhibit several of the pathogenic pathways that lead to cardiovascular disease. new data indicate that this extract has a significantly greater effect than either red wine or red-wine powder on the inhibition of platelet aggregation in vitro. based on this data, about 300 - 500 mg of the extract is equivalent to the daily dose of red-wine polyphenols that appears to prot ... | 2017 | 9818783 |
| changes in cell wall composition during ripening of grape berries | cell walls were isolated from the mesocarp of grape (vitis vinifera l.) berries at developmental stages from before veraison through to the final ripe berry. fluorescence and light microscopy of intact berries revealed no measurable change in cell wall thickness as the mesocarp cells expanded in the ripening fruit. isolated walls were analyzed for their protein contents and amino acid compositions, and for changes in the composition and solubility of constituent polysaccharides during developmen ... | 1998 | 9808722 |
| antidotal effect of grape juice (vitis vinifera) on ochratoxin a caused hepatorenal carcinogenesis in mice (mus musculus). | oral administration of ochratoxin a to young weanling mice (mus musculus) caused several haematological changes and induced hepatoma and renal carcinoma. concurrent administration of berry and leaf juice of the common grape (vitis vinifera) to mice together with ochratoxin a significantly reduced the hepatic and renal damage caused by ingestion of this mycotoxin. none of the animals receiving berry/leaf juice of v. vinifera showed the formation of hepatorenal carcinoma whereas 25% of animals rec ... | 1998 | 9734345 |
| population diversity in grapevine yellow speckle viroid-1 and the relationship to disease expression. | vitis vinifera cultivars zinfandel-1a and mission were found to harbor different grapevine yellow speckle viroid-1 (gysvd-1) variants and characterized to define the relationship to yellow speckle (ys) and vein-banding (vb) diseases. products from the left terminal (t1), pathogenic (p), and a portion of the central (c) domains of zinfandel-1a and mission displayed distinct single-stranded conformation polymorphism (sscp) patterns, presumably reflecting nucleotide changes in the p domain. the two ... | 1998 | 9721250 |
| structure of a hemicellulose b fraction in dietary fiber from the seed of grape variety palomino (vitis vinifera cv. palomino). | the structure of one of the hemicellulose b fractions (hb-1) extracted from the seeds of the grape variety palomino (vitis vinifera cv. palomino) has been studied by means of methylation analysis, 1h nmr, 13c nmr, and partial acid hydrolysis. this hemicellulose seems to be a homogeneous polysaccharide with an apparent molecular weight of 35 000. its structure is that of an acidic arabinoxylan, a linear chain of beta-d-xylopyranosyl units, bonded together by (1-->4) glycosidic links, containing a ... | 1998 | 9677268 |
| structure of a hemicellulose a fraction in dietary fiber from the seed of grape variety palomino (vitis vinifera cv. palomino). | the structure of one of the hemicellulose a fractions (ha-1) extracted from the seeds of the grape variety palomino (vitis vinifera cv. palomino) has been studied by means of methylation, 1h nmr, 13c nmr, and partial hydrolysis. this hemicellulose seems to be a homogeneous polysaccharide with an apparent molecular weight of 68 500. its structure is that of an acidic xylan, a linear chain of beta-d-xylopyranosyl units, bonded together by (1-->4) glycosidic links, containing single alpha-d-xylopyr ... | 1998 | 9677267 |
| establishment of an agrobacterium-mediated transformation system for grape (vitis vinifera l.): the role of antioxidants during grape-agrobacterium interactions. | very short exposures of embryogenic calli of vitis vinifera cv. superior seedless grape plants to diluted cultures of agrobacterium resulted in plant tissue necrosis and subsequent cell death. antibiotics used for agrobacterium elimination or as plant selectable markers were not responsible for this necrotic response. rather, cell death seemed to be oxygen-dependent and correlated with elevated levels of peroxides. therefore, we studied the effects on necrosis of various combinations of antioxid ... | 1996 | 9630955 |
| sparing effect of procyanidins from vitis vinifera on vitamin e: in vitro studies. | the sparing/recycling effect of a highly purified, high molecular weight fraction of catechin oligomers (procyanidins) from vitis vinifera seeds on alpha-tocopherol was studied in both homogeneous solution and in heterogeneous phase (phosphatidylcholine liposomes and red blood cells). by hplc and electron spin resonance (esr) spectroscopy we evidenced that tocopheroxyl radical, induced by reaction of alpha-tocopherol with the stable radical dpph (2,2-diphenyl-1-picrylhydrazyl) is recycled by pro ... | 1998 | 9619118 |
| isolation, identification, and antioxidant activity of three stilbene glucosides newly extracted from vitis vinifera cell cultures | suspension cultures of vitis vinifera l. (vitaceae) produce many hydroxylated stilbene glucosides found in red wine. from these cells, we isolated and characterized glycosylated stilbenes, (z)-piceatannol (3,5,3',4'-tetrahydroxystilbene) -3-o-beta-d-glucopyranoside (6) and (e)- and (z)-resveratrol (3,5, 4'-trihydroxystilbene)-4'-o-beta-d-glucopyranoside (2 and 7, respectively), which have not previously reported to be constituents of vitis vinifera or wine. the ability of these compounds to act ... | 1998 | 9599270 |
| cloning and characterization of vitis vinifera udp-glucose:flavonoid 3-o-glucosyltransferase, a homologue of the enzyme encoded by the maize bronze-1 locus that may primarily serve to glucosylate anthocyanidins in vivo. | we report here the cloning and optimized expression at 16 degrees c and the characterization of a vitis vinifera udp-glucose:flavonoid 3-o-glucosyltransferase, an enzyme responsible for a late step in grapevine anthocyanin biosynthesis. the properties of this and other udp-glucose:flavonoid 3-o-glucosyltransferases, homologues of the product encoded by the maize bronze-1 locus, are a matter of conjecture. the availability of a purified recombinant enzyme allowed for the unambiguous determination ... | 1998 | 9535914 |
| nucleotide sequence of grapevine (vitis vinifera) cdna similar to snap proteins. | a cdna clone (vs1) homologous to snap proteins was isolated from a grapevine cdna library. the cdna insert was 1167 bp long and contained a single open reading frame coding for 289 amino acids. the amino acid sequence of vs1 shows similarity (35%-45%) to snap proteins from various sources. | 1997 | 9522131 |
| comparative study of radical scavenger and antioxidant properties of phenolic compounds from vitis vinifera cell cultures using in vitro tests. | vitis vinifera cell suspensions were used to isolate and characterize the flavonoids (anthocyanins, catechins) and non-flavonoids (stilbenes) found in red wine. furthermore, we showed that astringin is produced although this stilbene has not previously been reported to be a constituent of v. vinifera or wine. the ability of these compounds to act as radical scavengers was investigated using 1,1-diphenyl-2-picryl-hydrazyl (dpph), a stable free radical. antioxidant activities were assessed by thei ... | 1997 | 9395251 |
| characterization and expression of caffeoyl-coenzyme a 3-o-methyltransferase proposed for the induced resistance response of vitis vinifera l. | cell-suspension cultures of vitis vinifera l. cv pinot noir accumulated resveratrol upon fungal elicitation, and the activity of s-adenosyl-l-methionine:trans-caffeoyl-coenzyme a 3-o-methyl-transferase (ccoaomt), yielding feruloyl-coa, increased to a transient maximum at 12 to 15 h. ccoaomt cdna was cloned from the elicited cells and was shown to encode a polypeptide highly homologous to ccoaomts from cells of petroselinum species or zinnia species. the expression of the cdna in escherichia coli ... | 1997 | 9390437 |
| differential expression of chitinases in vitis vinifera l. responding to systemic acquired resistance activators or fungal challenge. | the concept of systemic acquired resistance (sar) enables a novel approach to crop protection, and particular pathogenesis-related proteins, i.e. an acidic chitinase, have been classified as markers of the sar response. basic class i (vchit1b) and a class iii (vch3) chitinase cdnas were cloned from cultured vitis vinifera l. cv pinot noir cells and used to probe the induction response of grapevine cells to salicylic acid or yeast elicitor. furthermore, the cells were treated with the commercial ... | 1997 | 9390436 |
| polyphenols produced during red wine ageing. | over the past few years, it has been accepted that a moderate red wine consumption is a factor beneficial to human health. indeed, people of france and italy, the two major wine-producing european countries, eat a lot of fatty foods but suffer less from fatal heart strokes than people in north-america or in the northern regions of europe, where wine is not consumed on a regular basis. for a time, ethanol was thought to be the "good" chemical species hiding behind what is known as the "french par ... | 1997 | 9388306 |
| a comparison of the hydroxyl radical scavenging properties of the shark bile steroid 5 beta-scymnol and plant pycnogenols. | the hydroxyl radical (oh.) quenching abilities of the following compounds were compared in the deoxyribose degradation system (initiated by the ferrous-ascorbate fenton reaction): (a) 5 beta-scymnol, the hepatoprotective shark bile sterol, and its mono- and di-sulfate esters; (b) three marketed pycnogenol preparations (syn: proanthocyanidin--natural plant-derived polyphenolic bioflavonoids) extracted from pine tree (pinus maritima) bark and grape (vitis vinifera) seeds; and (c) two known hydroxy ... | 1997 | 9305543 |
| a class iv chitinase is highly expressed in grape berries during ripening. | chitinase activity increased markedly at the onset of ripening in grape (vitis vinifera l.) berries and continued to increase throughout the sugar accumulation phase of berry development. in contrast, beta-1,3-glucanase activity was not detected in grape berries at any stage of development. two closely related chitinase cdnas (vvchi4a and vvchi4b) were cloned from grapes. sequence and southern analysis indicate that these two clones may represent alleles of the same gene. the predicted proteins ... | 1997 | 9232868 |
| identification and characterization of a fruit-specific, thaumatin-like protein that accumulates at very high levels in conjunction with the onset of sugar accumulation and berry softening in grapes. | the protein composition of the grape (vitis vinifera cv muscat of alexandria) berry was examined from flowering to ripeness by gel electrophoresis. a protein with an apparent molecular mass of 24 kd, which was one of the most abundant proteins in extracts of mature berries, was purified and identified by amino acid sequence to be a thaumatin-like protein. combined cdna sequence analysis and electrospray mass spectrometry revealed that this protein, vvtl1 (for v. vinifera thaumatin-like protein 1 ... | 1997 | 9232867 |
| uptake and transformation of benzene and toluene by plant leaves. | the [1-6(14)c]benzene and [1-(14)c]toluene vapors penetrate into hypostomatous leaves of acer campestre, malus domestica, and vitis vinifera from both sides, whereas hydrocarbons are more intensively absorbed by the stomatiferous side and more actively taken up by young leaves. benzene and toluene conversion in leaves occurs with the aromatic ring cleavage and their carbon atoms are mainly incorporated into nonvolatile organic acids, while their incorporation into amino acids is less intensive. ... | 1997 | 9212332 |
| antioxidant activity of the stilbene astringin, newly extracted from vitis vinifera cell cultures. | 1997 | 9191572 | |
| the parentage of a classic wine grape, cabernet sauvignon. | the world's great wines are produced from a relatively small number of classic european cultivars of vitis vinifera l most are thought to be centuries old and their origins have long been the subject of speculation. among the most prominent of these cultivars is cabernet sauvignon, described as "the world's most renowned grape variety for the production of fine red wine". although now grown in many countries, cabernet sauvignon derives its fame from its long association with the bordeaux region ... | 1997 | 9140400 |
| [the genetic polymorphism of the grape studied by rapd analysis]. | genetic polymorphism in plant of 3 genera of vitaceae family, 11 species of vitis genera, 10 cultivars of vitis vinifera and 4 cultivars that obtained from distant hybridization was studied. according to date of rapd analysis genetic distances were determined and phylogenetic dendrogram was constructed. the possibility of using pcr for evaluation of breeding material by the levels of genetic relations was shown. | 2008 | 9139436 |
| procyanidines from vitis vinifera seeds protect rabbit heart from ischemia/reperfusion injury: antioxidant intervention and/or iron and copper sequestering ability. | an isolated rabbit heart langendorff preparation paced electrically was used to evaluate the effects of a highly purified, high molecular weight fraction of oligomeric procyanidines isolated from vitis vinifera seeds on myocardial reperfusion injury after 40 minutes of low flow (1 ml/min) ischemia. infusion of the heart with 100 or 200 micrograms/ml procyanidines dose-dependently reduced ventricular contracture during ischemia (lvedp values decreased by 28% and 51%), decreased coronary perfusion ... | 1996 | 9000880 |
| mono- and diglycosides of (e)-6,9-dihydroxymegastigma-4,7-dien-3-one in vitis vinifera wine. | two beta-d-glucopyranosides and two 6-o-beta-d-apiofuranosyl-beta-d-glucopyranosides of (e)-6,9-dihydroxymegastigma-4,7-dien-3-one were isolated from vitis vinifera cv. gewürztraminer wine and their structures were established by nmr spectroscopy. | 1996 | 8987582 |
| characterization of vitis vinifera l. glutamine synthetase and molecular cloning of cdnas for the cytosolic enzyme. | grapevine (vitis vinifera l.) glutamine synthetase (gs) was analysed into two distinct classes of isoforms; one of them was present in both leaf and root tissues while the other one showed leaf specificity. western blot analysis revealed that grapevine gs consists of three types of polypeptides of distinct size and differential tissue specificity. two structurally distinct cdna clones, pgs1;1 and pgs1;2, encoding grapevine gs were isolated from a cell suspension library and characterized. both c ... | 1996 | 8843941 |
| sugar accumulation in grape berries. cloning of two putative vacuolar invertase cdnas and their expression in grapevine tissues. | during grape berry (vitis vinifera l.) ripening, sucrose transported from the leaves is accumulated in the berry vacuoles as glucose and fructose. to study the involvement of invertase in grape berry ripening, we have cloned two cdnas (gin1 and gin2) from berries. the cdnas encode translation products that are 62% identical to each other and both appear to be vacuolar forms of invertase. both genes are expressed in a variety of tissues, including berries, leaves, roots, seeds, and flowers, but t ... | 1996 | 8685267 |
| the amino-acid sequence similarity of plant glutamate dehydrogenase to the extremophilic archaeal enzyme conforms to its stress-related function. | a cdna clone encoding grapevine (vitis vinifera l. cv sultanina) nad(h)-glutamate dehydrogenase (gdh) was isolated from a cdna expression library by immunoscreening with a polyclonal antibody raised against grapevine gdh. nucleotide sequence analysis revealed an open reading frame (orf) encoding a precursor protein of 411 amino acids (aa) with a calculated molecular mass of 44.517 kda. the deduced aa sequence showed relatively higher homology to gdh from archaebacteria species, than to those fro ... | 1996 | 8626071 |
| variation of viroid profiles in individual grapevine plants: novel grapevine yellow speckle viroid 1 mutants show alterations of hairpin i. | this is the first report which gives a general survey about viroid variant composition in a vineyard and within single plants. a german vineyard with 20-year-old grapevines (vitis vinifera) of the cultivars 'bacchus' and 'kerner' was analysed for viroid infections. only grapevine yellow speckle viroid 1 (gysvd1) and the grapevine isolate of hop stunt viroid (hsvdg) were detected. both viroids occur in several sequence variations. eighteen novel gysvd1 variants and two previously published hsvdg ... | 1996 | 8558124 |
| disease resistance results from foreign phytoalexin expression in a novel plant. | although phytoalexins have long been inferred to be important in the defence of plants against fungal infection, there are few reports showing that they provide resistance to infection. several plants, including grapevine, synthesize the stilbene-type phytoalexin resveratrol when attacked by pathogens. stilbenes with fungicidal potential are formed in several unrelated plant species, such as peanut (arachis hypogaea), grapevine (vitis vinifera) and pine (pinus sylvestris). stilbene biosynthesis ... | 1993 | 8421520 |
| control of citrinin caused nephrotoxicosis through aqueous leaf extract of vitis vinifera l., mercurious corrossivus and cortisone. | 1993 | 8359859 | |
| cloning and molecular analysis of structural genes involved in flavonoid and stilbene biosynthesis in grape (vitis vinifera l.). | genes involved in flavonoid and stilbene biosynthesis were isolated from grape (vitis vinifera l.). clones coding for phenylalanine ammonia-lyase (pal), chalcone synthase (chs), chalcone isomerase (chi), flavanone 3-hydroxylase (f3h), dihydroflavonol 4-reductase (dfr), leucoanthocyanidin dioxygenase (ldox) and udp glucose:flavonoid 3-o-glucosyl transferase (ufgt), were isolated by screening a cdna library, obtained from mrna from seedlings grown in light for 48 h using snapdragon (antirrhinum ma ... | 1994 | 8193299 |
| a narrow-bore hplc method for the identification and quantitation of free, conjugated, and bound polyamines. | a modified sensitive high-pressure liquid chromatography (hplc) method, using a narrow-bore column (2.1 x 200 mm, c-18, 5-microns particle size) with a methanol:water gradient (55-84%, v/v), is described for direct analysis of benzoylated free (s), soluble-conjugated (sh), and insoluble-bound (ph) polyamines in plant tissues. regression curves for each fraction of polyamines allow a precise quantitative determination of putrescine, cadaverine, spermidine, spermine, and agmatine in all fractions ... | 1993 | 8109737 |
| oxidative stress in recalcitrant tissue cultures of grapevine. | thiobarbituric acid reactive substances (tbars), and fluorescent compounds with spectral characteristics typical of products associated with oxidative stress in senescent and aging plant and animal cells, were detected in tissue cultures of the recalcitrant grapevine vitis vinifera l. cultivar, sultanina. these compounds increased during the early stages of dedifferentiation (callogenesis) of nodal stem explants. catalase activity was not detected in the original explant, but was induced during ... | 1994 | 8063199 |
| free radicals scavenging action and anti-enzyme activities of procyanidines from vitis vinifera. a mechanism for their capillary protective action. | the scavenging by procyanidines (polyphenol oligomers from vitis vinifera seeds, cas 85594-37-2) of reactive oxygen species (ros) involved in the onset (ho degrees) and the maintenance of microvascular injury (lipid radicals r degrees, ro degrees, roo degrees) has been studied in phosphatidylcholine liposomes (pcl), using two different models of free radical generation: a) iron-promoted and b) ultrasound-induced lipid peroxidation. in a) lipid peroxidation was assessed by determination of thioba ... | 1994 | 8024628 |
| the plant inorganic pyrophosphatase does not transport k+ in vacuole membrane vesicles multilabeled with fluorescent probes for h+, k+, and membrane potential. | it has been claimed that the inorganic pyrophosphatase (ppase) of the plant vacuolar membrane transports k+ in addition to h+ in intact vacuoles (davies, j. m., poole, r. j., rea, p. a., and sanders, d. (1992) proc. natl. acad. sci. u.s.a. 89, 11701-11705). since this was not confirmed using the purified and reconstituted ppase consisting of a 75-kda polypeptide (sato, m.h., kasahara, m., ishii, n., homareda, h., matsui, h., and yoshida, m. (1994) j. biol. chem. 269, 6725-6728), these authors pr ... | 1995 | 7876200 |
| coat protein gene sequence of an austrian isolate of grapevine fanleaf virus. | the nucleotide sequence of the coat protein cistron of an austrian isolate of grapevine fanleaf virus (gflv-fc) from vitis vinifera cv. french colombard was determined. it shows small differences at the rna level as well as at the protein level compared to the sequences of already published grapevine fanleaf virus strains. the differences may be a result of the natural variation among virus populations or a consequence of selection in a special host plant. as the virus rna sequence reported here ... | 1995 | 7646340 |
| effect of fosetyl-a1 on peroxidase from grapevine (vitis vinifera) cells. | grapevine (vitis vinifera cv. monastrell) suspension cell cultures were treated with fosetyl-a1, a widely used systemic fungicide for grapevine diseases caused by oomycetes, and examined at the electron microscope level for peroxidase cytochemistry. the results showed that treatment with fosetyl-a1 provokes an activation of both vacuolar sap and tonoplast-located peroxidase, already described as due to the basic peroxidase isoenzyme, b5, which was previously characterized as a constitutive marke ... | 1995 | 7612959 |
| monitoring of grape harvesters for evidence of cholinesterase inhibition. | this report describes the results of a long-term monitoring study of 36 grape harvesters who were occupationally exposed to organophosphate pesticide residues following reentry into vineyards during september and october 1976. the study was designed to evaluate biochemical parameters associated with organophosphate pesticide residues. insignificant changes in blood cholinesterase activity were found. some workers had very low levels of organophosphate metabolites in the urine during the entire 6 ... | 1981 | 7265295 |
| the effect of atmospheric pollution on vitis vinifera l. pollen ultrastructure under natural conditions. | the ultrastructural modification of pollen grains in vitis vinifera l. variety and hybrids in areas of so2 atmospheric pollution (the main polluting so2 usually reaches 2.72 mg/m3), nitrogen oxide, and other gases derived from noniron metal processing factories have been studied. strains 1001 and 1002, resistant varieties, do not undergo ultrastructural modifications. neuburger and issabelle, medium resistant ones, have a heterogeneity in ultrastructural organization from normal forms to forms h ... | 1984 | 6734498 |
| [anthocyanosides extracted from vitis vinifera, vaccinium myrtillus and pinus maritimus. i. elastase-inhibiting activities in vitro. ii. compared angioprotective activities in vivo]. | 1983 | 6553084 | |
| assimilation of 3h in photosynthesizing leaves exposed to hto. | potato and wine grape plants were exposed for a period of 4 h to tritiated water vapor, and simultaneously to 14co2 which served as a tracer for photosynthetic assimilation. during and after exposure, foliar samples were collected in which the exchangeable and nonexchangeable fractions of organic 3h were determined, taking care that the exchangeable fraction should not be lost to atmosphere. it was demonstrated that about 20% of the organic h in vegetation could be exchanged with 3h of tissue wa ... | 1984 | 6511427 |
| mutagen content of table wines made from various grape species and hybrid cultivars. | the mutagen content of wines produced from the european vitis vinifera grapes was compared with that of wines produced from hybrids of v. vinifera and species indigenous to north america. mutagens were extracted on an xad-2 amberlite resin column and activated with s-9 and/or faecalase in the salmonella/microsomal mutagen assay. all white wines had insignificant mutagen levels. the only red wine to produce statistically significant reversion frequencies was that made from the concord grape. the ... | 1984 | 6373529 |
| relation of ringing to gibberellin-like activity in berries of vitis vinifera l. | 1965 | 5836339 | |
| gonadotropin promotion of adventitious root production on cuttings of begonia semperflorens and vitis vinifera. | adventitious rooting of begonia semperflorens cv. indian maid and vitis vinifera cv. semillon stem cuttings was significantly promoted by human chorionic gonadotropin (hcg). basal sections of hcg treated cuttings upon which promoted rooting took place had markedly less endogenous gibberellin (ga) activity than non-treated controls or apical sections of treated ones, while changes in auxin levels were not found. hcg also inhibited ga(3)-induced reducing sugar release from embryoless barley endosp ... | 1968 | 5641189 |
| [equipment for the promotion of germination of vine seeds (vitis vinifera l.) with sterile surfaces under sterile conditions]. | 1970 | 5537389 | |
| [sterilization of the surface of grape (vitis vinifera l.) seeds]. | 1970 | 5535934 | |
| [on the rhizosphere microflora of the grape vine (vitis vinifera ssp. sativa, proles pontica, subproles balcanica, a.m. negrul) "kadarka". ii. effects of antibiotics on the bacteria of rhizosphere and the growth of grape vine influenced by these bacteria (author's transl)]. | 1974 | 4480499 | |
| [on the rhizosphere microflora of the grape vine (vitis vinifera ssp. sativa, proles pontica, subproles balcanica. a.m. negrul) "kadarka". i. morphological, biochemical, and serological characterization of bacteria (author's transl)]. | 1974 | 4211535 | |
| separation of viroid rnas by cellulose chromatography indicating conformational distinctions. | a series of viroids and viroid-like rnas extracted from citron (citrus medica), grapevine (vitis vinifera), and avocado (persea americanum) displayed differential binding capacities to cellulose in the presence of ethanol. this nonionic interaction was influenced by the presence of magnesium ions suggesting variations in conformation among the viroid rnas and corresponding differences in reactivity with cellulose. the specific elution profiles present a basis for the subclassification of groups ... | 1986 | 3776103 |
| the effects of fruit juices and fruits on the absorption of iron from a rice meal. | the effects of the chemical composition of fruit juices and fruit on the absorption of iron from a rice (oryza sativa) meal were measured in 234 parous indian women, using the erythrocyte utilization of radioactive fe method. the corrected geometric mean fe absorptions with different juices varied between 0.040 and 0.129, with the variation correlating closely with the ascorbic acid contents of the juices (rs 0.838, p less than 0.01). ascorbic acid was not the only organic acid responsible for t ... | 1987 | 3593665 |
| nutritive value of different grape musts (vitis vinifera l.). | musts of 11 different grape sorts were analyzed for titratable acids, total sugars, nitrogen, vitamin b group (b1, b2, b6, pantothenic acid) and macro- and micro elements (k, ca, mg, fe, zn, cu, mn, mo). analyses were done on samples collected in 1983 and 1984. the nutritive value of each sample was characterised by indices based on the nutrient concentrations and recommended dietary allowances (rda). the nutritive values of the various grape must sorts were generally similar to those of the fol ... | 2012 | 3507685 |
| chromium and nickel in roadside grapes. | chromium and nickel concentration in roadside unwashed wine grapes and leaves is considered as a function of the distance from the suspected source, an isolated straight road. the analyses were performed with electrothermal atomization in a graphite furnace. the decrease in the concentration of the element can be described by means of a three-parameter exponential function c = a + (b - a)exp(-cd), which allows the determination of the asymptotic level far from the emitting source (c infinity) an ... | 2013 | 2924978 |
| [flavonoids of 3 cultivars vine leaves, vitis vinifera l. var. tinctoria (alicante, carignan, grand noir). value in chemical control]. | three flavonoids were isolated and identified from the leaves of 3 cultivars vine vitis vinifera l. var. tinctoria (alicante, carignan and grand noir): hyperin, isoquercitrin and quercetin 3-o-beta-d glucuronic acid. tlc analytic control and hplc determination are proposed in this paper. | 1989 | 2637643 |
| prenyltransferase compartmentation in cells of vitis vinifera cultivated in vitro. | two prenyltransferases were located in cell cultures of vitis vinifera. a geranyl pyrophosphate synthase (ec 2.5.1.1) was associated with plastid-like membranes whereas a farnesyl pyrophosphate synthase (ec 2.5.1.10) was found to be soluble. | 1990 | 2226808 |
| new polyphenolic 5'-nucleotidase inhibitors isolated from the wine grape "koshu" and their biological effects. | new 5'-nucleotidase inhibitors designated as npf-88bu-ia, npf-88bu-ib, npf-88bu-iia and npf-88bu-iib, respectively, were isolated from the seeds and skin of the wine grape "koshu". they were purified by solvent extraction, dialysis, and reversed-phase high performance liquid chromatography (hplc). their physico-chemical properties revealed these compounds to be polyphenolic substances. the average relative molecular masses of the four were estimated by gel permeation chromatography (gpc) analysi ... | 1991 | 1934168 |
| role of t-region borders in agrobacterium host range. | the limited host range ab3 strain of agrobacterium tumefaciens induces tumors by transferring two t-regions, ta and tb. ta is a deleted version of the well-known biotype i octopine tl-region that lacks the iaa and ipt genes, but carries an intact oncogene, gene 6b, and typical left and right border sequences. tb carries two iaa genes that together code for the synthesis of indoleacetic acid. gene 6b and the iaa gene act synergistically when transferred in a coinoculation experiment. the ta-regio ... | 1991 | 1932812 |
| antiexudative and capillaritonic effects of procyanidines isolated from grape seeds (v. vinifera). | a purified and enriched fraction, containing procyanidines, was isolated from grape seeds of bulgarian sorts of vitis vinifera. the effects of procyanidines on the local oedema, produced by subplantar injection of carrageenin and dextran in the hind rat paw, were studied. we also tested their effect on the capillary permeability using intravenous injection of evans blue and local irritation by xylene. procyanidines at a dose of 2 mg/kg applied orally three times daily for 6 days inhibited the ca ... | 1990 | 1714228 |
| polygalacturonase is a virulence factor in agrobacterium tumefaciens biovar 3. | agrobacterium tumefaciens biovar 3 causes both crown gall and root decay of grapes. all biovar 3 strains, regardless of their tumorigenicity, produce in culture a single polygalacturonase with a pi around 4.5. a. tumefaciens biovar 3 strain cg49 was mutagenized with tn5 by using psup2021 as a suicide vector. a mutant strain, cg50, lacking polygalacturonase activity was isolated. the mutation was due to a single tn5 insertion in an 8.5-kb ecori fragment that also contained the polygalacturonase s ... | 1991 | 1655716 |
| analytical methods for monoterpene glycosides in grape and wine. ii. qualitative and quantitative determination of monoterpene glycosides in grape. | free and glycosidically bound terpenes of five vitis vinifera grape cultivars (muscat of alexandria, muscat of frontignan, muscat of hamburg, muscat ottonel and gewürztraminer) were investigated. the free and bound fractions were separated by selective retention on amberlite xad-2 resin. the glycosidic fractions were analysed by gas chromatography and gas chromatography-mass spectrometry using either enzymic hydrolysis and subsequent analysis of the released aglycones or trimethylsilyl (tms) and ... | 1992 | 1577909 |
| ph-induced kinetic co-operativity of a thylakoid-bound polyphenol oxidase. | a study of the catecholase activity of a latent plant polyphenol oxidase, extracted and purified from the chloroplast membranes of grapes (vitis vinifera cv. airen), revealed for the first time a lag phase above ph 5.0, whereas a steady-state rate was reached immediately when ph values were lower, thus suggesting the hysteretic nature of the enzyme. during steady state, the enzyme showed negative co-operativity concomitant with the presence of the lag period, and followed classical michaelis-men ... | 1992 | 1530593 |
| oxidation of hydroquinone by both cellular and extracellular grapevine peroxidase fractions. | the oxidation of hydroquinone by two peroxidase (ec 1.11.1.7) fractions obtained from the cells and spent medium of cell cultures of grapevine (vitis vinifera cv monastrell) has been studied, and their comparative efficacy (kcat/km ratio) studied in both the h2o2-consuming and hydroquinone-consuming reactions. while the efficacy in the h2o2-consuming reaction is practically identical for both enzyme fractions, the cellular peroxidase has five-fold more efficacy in the hydroquinone-consuming reac ... | 1992 | 1316172 |
| [determination of an elisa test on tissues of grapevine affected by yellow disease]. | we report here results concerning the best part of the grapevine to section, the best sampling period and an important adaptation of the extraction media for the diagnosis of a grapevine yellow disease by elisa. the addition of triton-x100, or better of chaps enabled us to get clear results for the sensitive vitis vinifera scion varieties. until now, results have not been obtained for the symptomless root stock varieties. however, antigen concentration by molecular filtration may be a way to sol ... | 1992 | 1298032 |