Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| trace-level volatile quantitation by direct analysis in real time mass spectrometry following headspace extraction: optimization and validation in grapes. | ambient ionization mass spectrometric (ai-ms) techniques like direct analysis in real time (dart) offer the potential for rapid quantitative analyses of trace volatiles in food matrices, but performance is generally limited by the lack of preconcentration and extraction steps. the sensitivity and selectivity of ai-ms approaches can be improved through solid-phase microextraction (spme) with appropriate thin-film geometries, for example, solid-phase mesh-enhanced sorption from headspace (spmesh). ... | 2017 | 28965401 |
| deciphering the role of cbf/dreb transcription factors and dehydrins in maintaining the quality of table grapes cv. autumn royal treated with high co2 levels and stored at 0°c. | c-repeat/dehydration-responsive element binding factors (cbf/dreb) are transcription factors which play a role in improving plant cold stress resistance and recognize the dre/crt element in the promoter of a set of cold regulated genes. dehydrins (dhns) are proteins that accumulate in plants in response to cold stress, which present, in some cases, cbf/dreb recognition sequences in their promoters and are activated by members of this transcription factor family. the application of a 3-day gaseou ... | 2017 | 28970842 |
| a new version of the grapevine reference genome assembly (12x.v2) and of its annotation (vcost.v3). | 2017 | 28971018 | |
| antibacterial and antioxidant properties of grape stem extract applied as disinfectant in fresh leafy vegetables. | in the present study total phenolic content (tpc), total flavonoid content (tfc), antioxidant activity and antimicrobial properties of grape (vitis vinifera var. red globe) stem extract is reported. also, the identification of main phenolic compounds was carried out by uplc-pad analysis. tpc and tfc of extract were 37.25 g gae kg-1 and 98.07 g qe kg-1, respectively. extract showed an antioxidant capacity of 132.60 and 317 g te kg-1 for dpph and abts radical scavenging capacity, respectively. the ... | 2017 | 28974804 |
| cytotoxicity and apoptotic cell death induced by vitis vinifera peel and seed extracts in a431 skin cancer cells. | vitis vinifera. l is one of the most widely consumed fruits in the world and are rich in antioxidant abundant polyphenols. the present study was carried out to assess the antiproliferative and apoptotic effects of vitis vinifera peel and seed extracts in an in vitro model using human epidermoid carcinoma a431 cell lines. vitis vinifera peel and seed extracts were incubated with a431 cells to evaluate the antiproliferative, apoptotic effects and the morphological apoptotic changes induced by the ... | 2017 | 28983752 |
| use of endophytic and rhizosphere actinobacteria from grapevine plants to reduce nursery fungal graft infections that lead to young grapevine decline. | endophytic and rhizosphere actinobacteria isolated from the root system of 1-year-old grafted vitis vinifera plants were evaluated for their activities against fungi that cause grapevine trunk diseases. a total of 58 endophytic and 94 rhizosphere isolates were tested. based on an in vitro bioassay, 15.5% of the endophytic isolates and 30.8% of the rhizosphere isolates exhibited antifungal activity against the fungal pathogen diplodia seriata, whereas 13.8% of the endophytic isolates and 16.0% of ... | 2017 | 28986378 |
| in vino veritas: an invitation for ambitious, collaborative proteogenomics campaigns on plant and animal models. | vitis vinifera has been an emblematic plant for humans since the neolithic period. human civilization has been shaped by its domestication as both its medicinal and nutritional values were exploited. it is now cultivated on all habitable continents, and more than 5000 varieties have been developed. a global passion for the art of wine fuels innovation and a profound desire for knowledge on this plant. the genome sequence of a homozygotic cultivar and several rna-seq datasets on other varieties h ... | 2017 | 28994197 |
| recombinant expression, purification, and characterization of polyphenol oxidase 2 (vvppo2) from "shine muscat" (vitis labruscana bailey × vitis vinifera l.). | polyphenol oxidases (ppos) catalyze browning reactions in various plant organs, therefore controlling the reactions is important for the food industry. ppos have been assumed to be involved in skin browning of white grape cultivars; however, the molecular mechanism underlying ppo-mediated browning process remains elusive. we have recently identified a new ppo gene named vvppo2 from "shine muscat" (vitis labruscana bailey × v. vinifera l.), and have shown that the gene is transcribed at a higher ... | 2017 | 29017399 |
| potassium in the grape (vitis vinifera l.) berry: transport and function. | k+ is the most abundant cation in the grape berry. here we focus on the most recent information in the long distance transport and partitioning of k+ within the grapevine and postulate on the potential role of k+ in berry sugar accumulation, berry water relations, cellular growth, disease resistance, abiotic stress tolerance and mitigating senescence. by integrating information from several different plant systems we have been able to generate new hypotheses on the integral functions of this pre ... | 2017 | 29021796 |
| identification of a novel vitivirus from grapevines in new zealand. | we report a sequence of a novel vitivirus from vitis vinifera obtained using two high-throughput sequencing (hts) strategies on rna. the initial discovery from small-rna sequencing was confirmed by hts of the total rna and sanger sequencing. the new virus has a genome structure similar to the one reported for other vitiviruses, with five open reading frames (orfs) coding for the conserved domains described for members of that genus. phylogenetic analysis of the complete genome sequence confirmed ... | 2018 | 29026999 |
| molecular typing of bois noir phytoplasma strains in the chianti classico area (tuscany, central italy) and their association with symptom severity in vitis vinifera 'sangiovese'. | bois noir (bn) is the most widespread disease of the grapevine yellows complex in the euro-mediterranean area. bn is caused by 'candidatus phytoplasma solani' (bnp), transmitted from herbaceous plants to grapevine by polyphagous insect vectors. in this study, genetic diversity among bnp strains and their prevalence and possible association with grapevine symptom severity were investigated in a sangiovese clone organic vineyard in the chianti classico area (tuscany). field surveys over 2 years re ... | 2018 | 29027886 |
| genome-wide characterization of differential transcript usage in arabidopsis thaliana. | alternative splicing and the usage of alternate transcription start- or stop sites allows a single gene to produce multiple transcript isoforms. most plant genes express certain isoforms at a significantly higher level than others, but under specific conditions this expression dominance can change, resulting in a different set of dominant isoforms. these events of differential transcript usage (dtu) have been observed for thousands of arabidopsis thaliana, zea mays and vitis vinifera genes, and ... | 2017 | 29031026 |
| effect of methyl jasmonate on the aroma of sangiovese grapes and wines. | methyl jasmonate (meja) was applied in a vineyard on leaves and grape clusters of cv sangiovese to test its ability to stimulate the production of aromas and identify the main genes involved in the biosynthetic pathways switched on by the elicitor. meja application led to a delay in grape technological maturity and a significant increase in the concentration of several berry aroma classes (about twice the total aroma: from around 3 to 6μg/g of berry). of these, monoterpenes showed the most signi ... | 2018 | 29037700 |
| involvement of ubiquitin-conjugating enzyme (e2 gene family) in ripening process and response to cold and heat stress of vitis vinifera. | ubiquitin-conjugating (ubc) e2 enzyme plays crucial roles in plant growth and development. limited information can describe the function of ubc enzyme e2 in grapes. a total of 43 ubc enzyme e2 genes with conserved ubc domain were identified in grapes. these genes were divided into five groups based on phylogenetic tree with tomatoes. sequence analyses indicated that vvubcs in the same group possessed similar gene structures and conserved motifs. gene distribution in chromosomes was uneven, and g ... | 2017 | 29038452 |
| evolutionary genomics of grape (vitis vinifera ssp. vinifera) domestication. | we gathered genomic data from grapes (vitis vinifera ssp. vinifera), a clonally propagated perennial crop, to address three ongoing mysteries about plant domestication. the first is the duration of domestication; archaeological evidence suggests that domestication occurs over millennia, but genetic evidence indicates that it can occur rapidly. we estimated that our wild and cultivated grape samples diverged ∼22,000 years ago and that the cultivated lineage experienced a steady decline in populat ... | 2017 | 29042518 |
| secreted proteins produced by fungi associated with botryosphaeria dieback trigger distinct defense responses in vitis vinifera and vitis rupestris cells. | grapevine trunk diseases (eutypa dieback, esca and botryosphaeria dieback) are caused by a complex of xylem-inhabiting fungi, which severely reduce yields in vineyards. botryosphaeria dieback is associated with botryosphaeriaceae. in order to develop effective strategies against botryosphaeria dieback, we investigated the molecular basis of grapevine interactions with a virulent species, neofusicoccum parvum, and a weak pathogen, diplodia seriata. we investigated defenses induced by purified sec ... | 2017 | 29043572 |
| chemical elements as fingerprints of geographical origin in cultivars of vitis vinifera l. raised on the same so4 rootstock. | the uptake of major and trace elements in grapevine (vitis vinifera l.) can be influenced by soil, climate, geographic origin, and rootstock type. rootstocks were mainly selected to resist phylloxera and for specific tolerance to lime, mineral uptake, drought, and salinity. the relationship among concentrations of major, trace, and rare earth elements was studied in soil and leaves from two italian grapevine cultivars, "cabernet sauvignon" and "corvina," employed to produce renowned controlled d ... | 2018 | 29047056 |
| vitis vinifera extract ameliorate hepatic and renal dysfunction induced by dexamethasone in albino rats. | this study was conducted to evaluate the biochemical effects of grape seed extract against dexamethasone-induced hepatic and renal dysfunction in a female albino rat. twenty-eight adult female rats were divided randomly into four equal groups: group 1: animals were injected subcutaneously with saline and consider as normal control one. group 2: animals were injected subcutaneously with dexamethasone in a dose of 0.1 mg/kg body weight. group 3: animals were injected subcutaneously with 0.1 mg/kg ... | 2017 | 29051443 |
| effects of different carrier materials on physicochemical properties of microencapsulated grape skin extract. | the goal of this study was to investigate the characteristics of grape skin extract (gse) spray dried with different carriers: maltodextrin (md), gum arabic (ga) and skim milk powder (smp). the grape skin extract was obtained from winery by-product of red grape variety prokupac (vitis vinifera l.). the morphology of the powders, their thermal, chemical and physical properties (water activity, bulk and tapped densities, solubility), as well as release studies in different ph conditions were analy ... | 2017 | 29051636 |
| comprehensive and comparative lipidome analysis of vitis vinifera l. cv. pinot noir and japanese indigenous v. vinifera l. cv. koshu grape berries. | vitis vinifera cv. koshu is an indigenous grape cultivar that has been cultivated for more than a thousand years in japan and one of the most important cultivars in white winemaking. to improve koshu wine quality, it is necessary to identify the metabolites in koshu berry. we conducted a comprehensive and comparative lipidome analysis of koshu and pinot noir berries cultivated in the same location in japan using gc-ms/ms for fatty acids and lc-ms for glycerolipids and glycerophospholipids. koshu ... | 2017 | 29053756 |
| chemical composition and sensory properties of non-wooded and wooded shiraz (vitis vinifera l.) wine as affected by vineyard row orientation and grape ripeness level. | the study aimed to unravel vineyard row orientation (ns, ew, ne-sw, nw-se) and grape ripeness level (23, 25, 27 °balling) implications for grape and wine composition and sensory properties/style (non-wooded/wooded wines) of vitis vinifera l. cv. shiraz (rootstock 101-14 mgt). | 2017 | 29077197 |
| impact of reduced atmospheric co2 and varied potassium supply on carbohydrate and potassium distribution in grapevine and grape berries (vitis vinifera l.). | to assess the robustness of the apparent sugar-potassium relationship during ripening of grape berries, a controlled-environment study was conducted on shiraz vines involving ambient and reduced (by 34%) atmospheric co2 concentrations, and standard and increased (by 67%) soil potassium applications from prior to the onset of ripening. the leaf net photoassimilation rate was decreased by 35% in the reduced co2 treatment. the reduction in co2 delayed the onset of ripening, but at harvest the sugar ... | 2017 | 29078143 |
| detailed phenolic composition of vidal grape pomace by ultrahigh-performance liquid chromatography-tandem mass spectrometry. | vidal blanc grape (vitis vinifera cv.) is the predominant white grape variety used for the production of icewine in china's liaoning province. in this paper, the development and validation of the method by ultrahigh-performance liquid chromatography-tandem mass spectrometry has been performed for determination of the detailed phenolic composition in the skin, seed and stem of vidal grapes. the validation of the method was realized by calculating the linearity, repeatability, precision, stability ... | 2017 | 29078146 |
| polyamine metabolism in climacteric and non-climacteric fruit ripening. | polyamines are small aliphatic amines that are found in both prokaryotic and eukaryotic organisms. these growth regulators have been implicated in abiotic and biotic stresses as well as plant development and morphogenesis. several studies have also suggested a key role of polyamines during fruit set and early development. polyamines have also been linked to fruit ripening and in the regulation of fruit quality-related traits.recent studies indicate that during ripening of both climacteric and no ... | 2018 | 29080186 |
| integrative field scale phenotyping for investigating metabolic components of water stress within a vineyard. | there is currently a high requirement for field phenotyping methodologies/technologies to determine quantitative traits related to crop yield and plant stress responses under field conditions. | 2017 | 29093742 |
| comparative genomic analysis of the pks genes in five species and expression analysis in upland cotton. | plant type iii polyketide synthase (pks) can catalyse the formation of a series of secondary metabolites with different structures and different biological functions; the enzyme plays an important role in plant growth, development and resistance to stress. at present, the pks gene has been identified and studied in a variety of plants. here, we identified 11 pks genes from upland cotton (gossypium hirsutum) and compared them with 41 pks genes in populus tremula, vitis vinifera, malus domestica a ... | 2017 | 29104824 |
| design of a custom rt-qpcr array for assignment of abiotic stress tolerance in traditional portuguese grapevine varieties. | widespread agricultural losses attributed to drought, often combined with high temperatures, frequently occur in the field, namely in mediterranean climate areas, where the existing scenarios for climate change indicate an increase in the frequency of heat waves and severe drought events in summer. grapevine (vitis vinifera l.) is the most cultivated fruit species in the world and the most valuable one and is a traditional mediterranean species. currently, viticulture must adjust to impending cl ... | 2017 | 29118776 |
| comparison of consecutive harvests versus blending treatments to produce lower alcohol wines from cabernet sauvignon grapes: impact on polysaccharide and tannin content and composition. | a changing climate has led to winegrapes being harvested with increased sugar levels and at greater risk of berry shrivel. a suggested easy-to-adopt strategy to manage the associated rising wine alcohol levels is the pre-fermentative substitution of juice with either "green harvest wine" or water. our study investigates the effects of this approach on vitis vinifera l. cv. cabernet sauvignon wine quality attributes. wines were also made from fruit collected at consecutive earlier harvest time po ... | 2018 | 29120804 |
| pest management under climate change: the importance of understanding tritrophic relations. | plants and insects depend on climatic factors (temperature, solar radiation, precipitations, relative humidity and co2) for their development. current knowledge suggests that climate change can alter plants and insects development and affect their interactions. shifts in tritrophic relations are of particular concern for integrated pest management (ipm), because responses at the highest trophic level (natural enemies) are highly sensitive to warmer temperature. it is expected that natural enemie ... | 2018 | 29127793 |
| epiphytic fungal community in vitis vinifera of the portuguese wine regions. | in this work, fungi present in the grapevine's phyllosphere collected from the main demarcated wine regions of portugal were identified, and their phylogenetic relationships were analysed. a total of 46 vine samples (leaves and berries) were collected from different parts of the country, being isolated a total of 117 fungal colonies that were identified to the genus level and sequenced in the following genetic regions: internal transcribed spacer region and 18s rrna and β-tubulin gene. next, a p ... | 2018 | 29139139 |
| dating the beginning of the roman viticultural model in the western mediterranean: the case study of chianti (central italy). | although domestication of the grapevine (vitis vinifera l.) has been extensively documented, the history of genotype selection and evolution of vineyard management remain relatively neglected fields of study. the find of 454 waterlogged grapevine pips from a well-dated etrusco-roman site in the chianti district (tuscany, central italy) is an extraordinary chance to gain insights into the progress of viticulture occurring in a key historical period in one of the world's most famous wine regions. ... | 2017 | 29140987 |
| partial solar radiation exclusion with color shade nets reduces the degradation of organic acids and flavonoids of grape berry (vitis vinifera l.). | the incidence of solar radiation on red-skinned grapes can promote the synthesis of flavonoids desirable for wine production, but elevated temperature may impair their accumulation. we performed a shade cloth trial covering the fruit zone (from pepper-corn size to maturity) with four polyethylene 1 m curtains with different optical properties (20% shading factor pearl colored and 40% shading factor aluminet, blue, and black colored) and a control with no cover. cluster temperature was 3.7 °c low ... | 2017 | 29141407 |
| constituents and antioxidant activity of bleeding sap from various xinjiang grapes. | wine grape sap or bleeding sap of grapes (gbs) is commonly used in xinjiang (china) for therapeutic aims. do variations in composition related to region and variety affect its properties? | 2017 | 29142440 |
| complete genome sequence of xanthomonas campestris pv. viticola strain ccrmxcv 80 from brazil. | here, we report the complete 5.3-mb genome sequence of xanthomonas campestris pv. viticola (ccrmxcv 80), which causes grapevine (vitis vinifera l.) bacterial canker. genome data will improve our understanding of the strain's comparative genomics and epidemiology, and help to further define plant protection and quarantine procedures. | 2017 | 29146856 |
| fungal planet description sheets: 558-624. | novel species of fungi described in this study include those from various countries as follows: australia: banksiophoma australiensis (incl. banksiophoma gen. nov.) on banksia coccinea, davidiellomycesaustraliensis (incl. davidiellomyces gen. nov.) on cyperaceae, didymocyrtis banksiae on banksia sessilis var. cygnorum, disculoides calophyllae on corymbia calophylla, harknessia banksiae on banksia sessilis, harknessia banksiae-repens on banksia repens, harknessia banksiigena on banksia sessilis v ... | 2017 | 29151634 |
| isolation, identification and selection of antagonistic yeast against alternaria alternata infection and tenuazonic acid production in wine grapes from argentina. | epiphytic isolates with yeast characteristics from grapes of the malbec cultivar were obtained in order to find antagonists against alternaria alternata. from a total of 111 isolates, 82% corresponded to the yeast-like organism aureobasidium pullulans and the rest to the non-saccharomyces yeasts hanseniaspora uvarum (6.3%), metschnikowia pulcherrima or spp. (5.4%), cryptoccocus laurentti ii (2.7%), starmerella bacilaris or candida zemplinina (2.7%) and rhodotorula spp. (0.9%). the 22.4% (15 out ... | 2017 | 29156243 |
| the lateral organ boundaries domain gene family in grapevine: genome-wide characterization and expression analyses during developmental processes and stress responses. | lateral organ boundaries (lob) domain (lbd) constitute a family of plant-specific transcription factors with key roles in the regulation of plant organ development, pollen development, plant regeneration, pathogen response, and anthocyanin and nitrogen metabolisms. however, the role of lbds in fruit ripening and in grapevine (vitis vinifera l.) development and stress responses is poorly documented. by performing a model curation of lbds in the latest genome annotation 50 genes were identified. p ... | 2017 | 29162903 |
| a randomized, double-blinded, clinical trial on effects of a vitis vinifera extract on cognitive function in healthy older adults. | introduction: gradual population aging is creating a new set of needs in the general population. memory capacity decreases with age, and memory deficits are considered an early symptom of alzheimer's disease (ad), one of the most prevalent cognitive disorders in older people. numerous studies have shown that grape polyphenolic compounds (gps) are able to attenuate cognitive impairment and reduce brain lesions in experimental ad animal models. these gp effects are associated with improvement in b ... | 2017 | 29163162 |
| wild grape-associated yeasts as promising biocontrol agents against vitis vinifera fungal pathogens. | the increasing level of hazardous residues in the environment and food chains has led the european union to restrict the use of chemical fungicides. thus, exploiting new natural antagonistic microorganisms against fungal diseases could serve the agricultural production to reduce pre- and post-harvest losses, to boost safer practices for workers and to protect the consumers' health. the main aim of this work was to evaluate the antagonistic potential of epiphytic yeasts against botrytis cinerea, ... | 2017 | 29163377 |
| cultivar diversity of grape skin polyphenol composition and changes in response to drought investigated by lc-ms based metabolomics. | phenolic compounds represent a large family of plant secondary metabolites, essential for the quality of grape and wine and playing a major role in plant defense against biotic and abiotic stresses. phenolic composition is genetically driven and greatly affected by environmental factors, including water stress. a major challenge for breeding of grapevine cultivars adapted to climate change and with high potential for wine-making is to dissect the complex plant metabolic response involved in adap ... | 2017 | 29163566 |
| global dna methylation patterns can play a role in defining terroir in grapevine (vitis vinifera cv. shiraz). | understanding how grapevines perceive and adapt to different environments will provide us with an insight into how to better manage crop quality. mounting evidence suggests that epigenetic mechanisms are a key interface between the environment and the genotype that ultimately affect the plant's phenotype. moreover, it is now widely accepted that epigenetic mechanisms are a source of useful variability during crop varietal selection that could affect crop performance. while the contribution of dn ... | 2017 | 29163587 |
| a comparison of petiole hydraulics and aquaporin expression in an anisohydric and isohydric cultivar of grapevine in response to water-stress induced cavitation. | we report physiological, anatomical and molecular differences in two economically important grapevine (vitis vinifera l.) cultivars cv. grenache (near-isohydric) and chardonnay (anisohydric) in their response to water-stress induced cavitation. the aim of the study was to compare organ vulnerability (petiole and stem) to cavitation by measuring ultrasonic acoustic emissions (uae) and percent loss of conductance of potted grapevines subject to the onset of water-stress. leaf (ψl) and stem water p ... | 2017 | 29163613 |
| comparison of benchtop fourier-transform (ft) and portable grating scanning spectrometers for determination of total soluble solid contents in single grape berry (vitis vinifera l.) and calibration transfer. | near-infrared (nir) spectroscopy was applied for the determination of total soluble solid contents (ssc) of single ruby seedless grape berries using both benchtop fourier transform (vector 22/n) and portable grating scanning (supnir-1500) spectrometers in this study. the results showed that the best ssc prediction was obtained by vector 22/n in the range of 12,000 to 4000 cm-1 (833-2500 nm) for ruby seedless with determination coefficient of prediction (rp²) of 0.918, root mean squares error of ... | 2017 | 29165336 |
| enological tannin effect on red wine color and pigment composition and relevance of the yeast fermentation products. | enological tannins are widely used in the winemaking process either to improve different wine characteristics (color stability, among others) or to compensate for low tannin levels. in this work, the influence of the addition of two different enological tannins, mainly composed of hydrolysable (ellagitannins) and condensed tannins, on the evolution of color and pigment composition of two different types of model systems containing the five main grape anthocyanins was studied. in addition, the ef ... | 2017 | 29168752 |
| harnessing the wine dregs: an approach towards a more sustainable synthesis of gold and silver nanoparticles. | in recent years, the management of food waste processing has emerged as a major concern. one such type of food waste, grape pomace, has been shown to be a great source of bioactive compounds which might be used for more environmentally - friendly processes for the synthesis of nanomaterials. in this study, grape pomace of vitis vinifera has been used for the obtainment of an aqueous extract. firstly, the reducing activity, total phenolic content and dpph scavenging activity of the aqueous extrac ... | 2018 | 29175604 |
| dissecting the variations of ripening progression and flavonoid metabolism in grape berries grown under double cropping system. | a double cropping system has been commercially adopted in southern china, where there is abundant sunshine and heat resources. in this viticulture system, the first growing season normally starts as a summer cropping cycle; then, the vine is pruned and forced, resulting in a second crop in winter. due to climate differences between the summer and winter growing seasons, grape ripening progression and flavonoid metabolism vary greatly. here, the metabolites and transcriptome of flavonoid pathways ... | 2017 | 29176986 |
| somatic embryogenesis from seeds in a broad range of vitis vinifera l. varieties: rescue of true-to-type virus-free plants. | somatic embryogenesis is the preferred method for cell to plant regeneration in vitis vinifera l. however, low frequencies of plant embryo conversion are commonly found. in a previous work we obtained from cut-seeds of a grapevine infected with the grapevine leafroll associated viruses 1 and 3 (glrav-1 and glrav-3), high rates of direct regeneration, embryo plant conversion and sanitation. the aim of this study is to evaluate the usefulness of this procedure for regeneration of other grapevine v ... | 2017 | 29187140 |
| plant dhdpr forms a dimer with unique secondary structure features that preclude higher-order assembly. | dihydrodipicolinate reductase (dhdpr) catalyses the second reaction in the diaminopimelate pathway of lysine biosynthesis in bacteria and plants. in contrast with the tetrameric bacterial dhdpr enzymes, we show that dhdpr from vitis vinifera (grape) and selaginella moellendorffii are dimeric in solution. in the present study, we have also determined the crystal structures of dhdpr enzymes from the plants arabidopsis thaliana and s. moellendorffii, which are the first dimeric dhdpr structures. th ... | 2018 | 29187521 |
| rna-seq-based genome annotation and identification of long-noncoding rnas in the grapevine cultivar 'riesling'. | the technological advances of rna-seq and de novo transcriptome assembly have enabled genome annotation and transcriptome profiling in highly heterozygous species such as grapevine (vitis vinifera l.). this work is an attempt to utilize a de novo-assembled transcriptome of the v. vinifera cultivar 'riesling' to improve annotation of the grapevine reference genome sequence. | 2017 | 29197332 |
| drosophila suzukii (diptera: drosophilidae) contributes to the development of sour rot in grape. | this research aimed to more clearly describe the interactions of drosophila suzukii (matsumura; diptera: drosophilidae) with microorganisms that may contribute to spoilage or quality loss of wine grapes during harvest. experiments were conducted in controlled laboratory experiments and under field conditions to determine these effects. laboratory trials determined the role of insect contact and oviposition to vector spoilage bacteria onto wine grapes. in the field, the roles of key organoleptic ... | 2017 | 29202199 |
| photoacoustic spectroscopy applied to the direct detection of bioactive compounds in agaricus brasiliensis mycelium. | this paper describes the application of the photoacoustic spectroscopic (pas) for detection of bioactive compounds in agaricus brasiliensis mycelium. the mycelium was cultivated by solid-state fermentation and by submerged fermentation. vegetal residues from food industry were used as substrates for fermentation: apple pomace (malus domestica), wheat (triticum aestivum), peel and pomace of pineapple (ananas comosus), malt (hordeum vulgare) and grape pomace (vitis vinifera). dry and ground sample ... | 2017 | 29210029 |
| phenolic content and in vitro antifungal activity of unripe grape extracts from agro-industrial wastes. | the antifungal activity of unripe grape extracts from agro-industrial wastes has been evaluated against several strains of candida spp. and dermatophytes. all the extracts tested showed antifungal activity. the geometric mean mic ranged from 53.58 to 214.31 μg/ml for candida spp. and from 43.54 to 133.02 μg/ml for dermatophytes. the chemical analyses have been carried out using liquid chromatograph equipped with a dad and ms detectors. flavan-3-ols were the main metabolites within all samples ra ... | 2017 | 29214894 |
| susceptibility of lobesia botrana (lepidoptera: tortricidae) to chlorantraniliprole in the emilia romagna region of northeast italy. | the european grapevine moth, lobesia botrana (denis & schiffermüller) (lepidoptera: tortricidae), is regarded as the most important insect pest of wine grapes in europe. if not properly controlled, it can cause significant direct and indirect yield losses due to secondary infections of grape berries by botrytis cinerea. for these reasons, it is important to preserve the activity of insecticides used against this pest, as the number of insecticidal mode of actions available to control lepidoptera ... | 2017 | 29228312 |
| dissecting the rootstock control of scion transpiration using model-assisted analyses in grapevine. | how rootstocks contribute to the control of scion transpiration under drought is poorly understood. we investigated the role of root characteristics, hydraulic conductance and chemical signals (abscisic acid, aba) in the response of stomatal conductance (gs) and transpiration (e) to drought in cabernet sauvignon (vitis vinifera) grafted onto drought-sensitive (vitis riparia) and drought-tolerant (vitis berlandieri × vitis rupestris 110r) rootstocks. all combinations showed a concomitant reductio ... | 2017 | 29228360 |
| whole-genome sequencing and snv genotyping of 'nebbiolo' (vitis vinifera l.) clones. | 'nebbiolo' (vitis vinifera) is among the most ancient and prestigious wine grape varieties characterised by a wide genetic variability exhibited by a high number of clones (vegetatively propagated lines of selected mother plants). however, limited information is available for this cultivar at the molecular and genomic levels. the whole-genomes of three 'nebbiolo' clones (cvt 71, cvt 185 and cvt 423) were re-sequenced and a de novo transcriptome assembly was produced. important remarks about the ... | 2017 | 29229917 |
| elimination of grapevine leafroll associated virus-3, grapevine rupestris stem pitting associated virus and grapevine virus a from a tunisian cultivar by somatic embryogenesis and characterization of the somaclones using ampelographic descriptors. | prospecting of local grapevine (vitis vinifera l.) germplasm revealed that tunisia possesses a rich patrimony which presents diversified organoleptic characteristics. however, viral diseases seriously affect all local grapevine cultivars which risk a complete extinction. sanitation programs need to be established to preserve and exploit, as a gene pool, the tunisian vineyards areas. the presence of the grapevine leafroll associated virus-3 (glrav-3), grapevine stem pitting associated virus (grsp ... | 2017 | 29238279 |
| breeding next generation tree fruits: technical and legal challenges. | the new plant breeding technologies (npbts) have recently emerged as powerful tools in the context of 'green' biotechnologies. they have wide potential compared to classical genetic engineering and they are attracting the interest of politicians, stakeholders and citizens due to the revolutionary impact they may have on agriculture. cisgenesis and genome editing potentially allow to obtain pathogen-resistant plants or plants with enhanced qualitative traits by introducing or disrupting specific ... | 2017 | 29238598 |
| combined enzymatic and metabolic analysis of grapevine cell responses to elicitors. | elicitors trigger plant defense responses, including phytoalexin production and cell-wall reinforcement. primary metabolism plays an important role in these responses as it fuels the associated energetic costs and provides precursors for the synthesis of the numerous secondary metabolites involved in defenses against pathogens. in this context, we aimed to determine whether oligosaccharidic elicitors differing in their capacity to activate defense-associated secondary metabolism in grapevine wou ... | 2017 | 29241147 |
| nmr-based phytochemical analysis of vitis vinifera cv falanghina leaves. characterization of a previously undescribed biflavonoid with antiproliferative activity. | vitis vinifera cv falanghina is an ancient grape variety of southern italy. a thorough phytochemical analysis of the falanghina leaves was conducted to investigate its specialised metabolite content. along with already known molecules, such as caftaric acid, quercetin-3-o-β-d-glucopyranoside, quercetin-3-o-β-d-glucuronide, kaempferol-3-o-β-d-glucopyranoside and kaempferol-3-o-β-d-glucuronide, a previously undescribed biflavonoid was identified. for this last compound, a moderate bioactivity agai ... | 2017 | 29242038 |
| genome-wide assessment of polygalacturonases-like (pgl) genes of medicago truncatula, sorghum bicolor, vitis vinifera and oryza sativa using comparative genomics approach. | the polygalacturonases (pg) is one of the important members of pectin-degrading glycoside hydrolases of the family gh28. in plants, pg represents multigene families associated with diverse processes. in the present study, an attempt has been made to investigate the diversity of pg genes among monocots and dicots with respect to phylogeny, gene duplication and subcellular localization to get an insight into the evolutionary and functional attributes. the genome-wide assessment of medicago truncat ... | 2017 | 29243204 |
| post-veraison irreversible stem shrinkage in grapevine (vitis vinifera) is caused by periderm formation. | grapevines are characterized by a period of irreversible stem shrinkage around the onset of ripening of the grape berries. since this shrinkage is unrelated to meteorological conditions or drought, it is often suggested that it is caused by the increased sink strength of the grape berries during this period. however, no studies so far have experimentally investigated the mechanisms underlying this irreversible stem shrinkage. we therefore combined continuous measurements of stem diameter variati ... | 2017 | 29244181 |
| quantitating volatile phenols in cabernet franc berries and wine after on-vine exposure to smoke from a simulated forest fire. | smoke-taint is a wine defect linked to organoleptic volatile phenols (vps) in vitis vinifera l. berries that have been exposed to smoke from wildland fires. herein, the levels of smoke-taint-associated vps are reported in cabernet franc berries from veraison to commercial maturity and in wine after primary fermentation following on-vine exposure to simulated wildland fire smoke. vps increased after smoke exposure were rapidly stored as acid-labile conjugates, and the levels of both free vps and ... | 2018 | 29244496 |
| differential expression of vvloxa diversifies c6 volatile profiles in some vitis vinifera table grape cultivars. | c6 volatiles are synthesized through lipoxygenase-hydroperoxide lyase (lox-hpl) pathway and these volatiles play important roles in the aromatic quality of grape berries. this study investigated the evolution of both c6 volatiles and the key genes in the lox-hpl pathway in different table grape cultivars during the berry development period, and further assessed the correlation between the accumulation of c6 volatiles and the expression of these genes in these cultivars. results showed that hexan ... | 2017 | 29261101 |
| tailoring tobacco hairy root metabolism for the production of stilbenes. | tobacco hairy root (hr) cultures, which have been widely used for the heterologous production of target compounds, have an innate capacity to bioconvert exogenous t-resveratrol (t-r) into t-piceatannol (t-pn) and t-pterostilbene (t-pt). we established genetically engineered hr carrying the gene encoding stilbene synthase (sts) from vitis vinifera and/or the transcription factor (tf) atmyb12 from arabidopsis thaliana, in order to generate a holistic response in the phenylpropanoid pathway and coo ... | 2017 | 29269790 |
| characterization of ugt716a1 as a multi-substrate udp:flavonoid glucosyltransferase gene in ginkgo biloba. | ginkgo biloba l., a "living fossil" and medicinal plant, is a well-known rich source of bioactive flavonoids. the molecular mechanism underlying the biosynthesis of flavonoid glucosides, the predominant flavonoids in g. biloba, remains unclear. to better understand flavonoid glucosylation in g. biloba, we generated a transcriptomic dataset of g. biloba leaf tissue by high-throughput rna sequencing. we identified 25 putative udp-glycosyltransferase (ugt) unigenes that are potentially involved in ... | 2017 | 29270187 |
| aroma modulation of cabernet gernischt dry red wine by optimal enzyme treatment strategy in winemaking. | cabernet gernischt (cg) is a famous chinese wine grape cultivar, the red wine of which is known for its green trait, especially when produced from grapes cultivated in regions with monsoon climate. to modify cg wine aroma, three enzyme preparations (h. uvarum extracellular enzyme, ar2000, and pectinase) were introduced in different winemaking stages with saccharomyces cerevisiae. free and bound aroma compounds in young wines were detected using headspace solid-phase micro-extraction and gas chro ... | 2018 | 29287349 |
| anti-lipase potential of the organic and aqueous extracts of ten traditional edible and medicinal plants in palestine; a comparison study with orlistat. | background: herbs have played a fundamental and essential role in the humans life since ancient times, especially those which are used as food and/or folk medicinedue to both their nutritive and curative properties.this study aimed to investigate new antilipase agents from tentraditional palestinian edible and medicinal plants through inhibition of the absorption of dietary lipids. methods: the anti-lipase activity for ten plants was evaluated and compared with the reference compound orlistat by ... | 2017 | 29292744 |
| a 3-d functional-structural grapevine model that couples the dynamics of water transport with leaf gas exchange. | predicting both plant water status and leaf gas exchange under various environmental conditions is essential for anticipating the effects of climate change on plant growth and productivity. this study developed a functional-structural grapevine model which combines a mechanistic understanding of stomatal function and photosynthesis at the leaf level (i.e. extended farqhuhar-von caemmerer-berry model) and the dynamics of water transport from soil to individual leaves (i.e. tardieu-davies model). | 2017 | 29293870 |
| anthocyanin management in fruits by fertilization. | anthocyanins are water-soluble vacuolar plant pigments that are mainly synthesized in epidermal layers and the flesh of fruits such as apples, cherries, grapes, and other berries. because of their attractive red to purple coloration and their health-promoting potential, anthocyanins are significant determinants for the quality and market value of fruits and fruit-derived products. in crops, anthocyanin accumulation in leaves can be caused by nutrient deficiency which is usually ascribed to insuf ... | 2018 | 29297687 |
| combining color chart, colorimetric measurement and chemical compounds for postharvest quality of white wine grapes. | this paper provides data for the potential use of a color chart to establish the best quality of white wine grape destined to postharvest processing. grechetto, vermentino and muscat of alexandria white wine varieties, were tested by sampling berries at different dates along quality attributes evolution. a color chart and reflectance spectrocolorimeter were used in combination with analyses of total carotenoids and chlorophylls in all three varieties and volatile organic compounds (vocs) for gre ... | 2018 | 29297926 |
| grapevine rootstocks shape underground bacterial microbiome and networking but not potential functionality. | the plant compartments of vitis vinifera, including the rhizosphere, rhizoplane, root endosphere, phyllosphere and carposphere, provide unique niches that drive specific bacterial microbiome associations. the majority of phyllosphere endophytes originate from the soil and migrate up to the aerial compartments through the root endosphere. thus, the soil and root endosphere partially define the aerial endosphere in the leaves and berries, contributing to the terroir of the fruit. however, v. vinif ... | 2018 | 29298729 |
| protective effects of red grape (vitis vinifera) juice through restoration of antioxidant defense, endocrine swing and hsf1, hsp72 levels in heat stress induced testicular dysregulation of wister rat. | ability of red grape juice (rgj), a known antioxidant, on testis of adult wister rat to protect from oxidative stress induced damages by heat stress has been investigated in this study. heat stress was induced maintaining body and testicular temperature at 43°c for 30min/day for 15 days using a hyperthermia induction chamber. four groups of rats (n=6 per group) comprising of group-i (control) -kept at 32°c, group-ii -exposed to heat stress alone, group-iii received rgj (0.8ml/rat/day) alone and ... | 2018 | 29301698 |
| genetic architecture of aerial and root traits in field-grown grafted grapevines is largely independent. | qtls were identified for traits assessed on field-grown grafted grapevines. root number and section had the largest phenotypic variance explained. genetic control of root and aerial traits was independent. breeding new rootstocks for perennial crops remains challenging, mainly because of the number of desirable traits which have to be combined, these traits include good rooting ability and root development. consequently, the present study analyzes the genetic architecture of root traits in grape ... | 2018 | 29305700 |
| exogenous 24-epibrassinolide interacts with light to regulate anthocyanin and proanthocyanidin biosynthesis in cabernet sauvignon (vitis vinifera l.). | anthocyanins and proanthocyanidins (pas) are crucial factors that affect the quality of grapes and the making of wine, which were stimulated by various stimuli and environment factors (sugar, hormones, light, and temperature). the aim of the study was to investigate the influence of exogenous 24-epibrassinolide (ebr) and light on the mechanism of anthocyanins and pas accumulation in grape berries. grape clusters were sprayed with ebr (0.4 mg/l) under light and darkness conditions (ebr + l, ebr + ... | 2018 | 29315208 |
| european ethnopharmaceuticals for self-medication in japan: review experience of vitis vinifera l., folium extract and vitex agnus-castus l., fructus extract as otc drugs. | since the publication of "application guideline for western traditional herbal medicines as otc drugs" in 2007, only two european ethnopharmaceuticals, vitis vinifera l., folium extract (antistax) and vitex agnus-castus l., fructus extract (prefemin), have been approved as otc drugs in japan. in this review, we describe the current regulation of western ethnopharmaceuticals in japan, summarize our regulatory experiences and discuss the scientific and regulatory issues involved. | 2018 | 29316611 |
| isolation of proanthocyanidins from red wine, and their inhibitory effects on melanin synthesis in vitro. | the red wines made from vitis vinifera were identified as skin-whitening effectors by using in vitro assays. opcs in the wine were evaluated for tyrosinase activity and melanogenesis. strong tyrosinase inhibitory activity was observed in fractions with high oligomeric proanthocyanidin (opc) content. among opc dimers, a strong inhibitory effect on tyrosinase was observed with opcs which contain (+)-catechin as an upper unit. melanogenesis inhibitory effect was observed with opcs which have (-)-ep ... | 2018 | 29329871 |
| a multi-omics study of the grapevine-downy mildew (plasmopara viticola) pathosystem unveils a complex protein coding- and noncoding-based arms race during infection. | fungicides are applied intensively to prevent downy mildew infections of grapevines (vitis vinifera) with high impact on the environment. in order to develop alternative strategies we sequenced the genome of the oomycete pathogen plasmopara viticola causing this disease. we show that it derives from a phytophthora-like ancestor that switched to obligate biotrophy by losing genes involved in nitrogen metabolism and γ-aminobutyric acid catabolism. by combining multiple omics approaches we characte ... | 2018 | 29335535 |
| developmental control of hypoxia during bud burst in grapevine. | dormant or quiescent buds of woody perennials are often dense, and in the case of grapevine (vitis vinifera l.) have a low tissue oxygen status. the precise timing of the decision to resume growth is difficult to predict, but once committed the increase in tissue oxygen status is rapid and developmentally regulated. here we show that more than a third of the grapevine homologues of widely conserved hypoxia-responsive genes, and nearly a fifth of all grapevine genes possessing a plant hypoxia-res ... | 2018 | 29336037 |
| molecular analysis of anthocyanin biosynthesis pathway genes and their differential expression in mango peel. | mango fruit is cherished by masses for its taste and nutrition, contributed by color, flavor and aroma. among these, peel color is an important trait contributing to fruit quality and market value. we attempted to elucidate the role of key genes of anthocyanin biosynthesis pathway related to fruit peel color from the leaf transcriptome of cv. amrapali. a total of 108 mined transcript sequences were assigned to phenylpropanoid flavonoid pathway from which 15 contigs representing anthocyanin biosy ... | 2018 | 29338343 |
| a new image-based tool for the high throughput phenotyping of pollen viability: evaluation of inter- and intra-cultivar diversity in grapevine. | low pollen viability may limit grapevine yield under certain conditions, causing relevant economic losses to grape-growers. it is usually evaluated by the quantification of the number of viable and non-viable pollen grains that are present in a sample after an adequate pollen grain staining procedure. although the manual counting of both types of grains is the simplest and most sensitive approach, it is a laborious and time-demanding process. in this regard, novel image-based approaches can assi ... | 2018 | 29339970 |
| comparison of three assembly strategies for a heterozygous seedless grapevine genome assembly. | de novo heterozygous assembly is an ongoing challenge requiring improved assembly approaches. in this study, three strategies were used to develop de novo vitis vinifera 'sultanina' genome assemblies for comparison with the inbred v. vinifera (pn40024 12x.v2) reference genome and a published sultanina allpaths-lg assembly (ap). the strategies were: 1) a default platanus assembly (plat_d) for direct comparison with ap assembly, 2) an iterative merging strategy using metassembler to combine plat_d ... | 2018 | 29343235 |
| efficacy of grape seed extract gel in the treatment of chronic periodontitis: a randomized clinical study. | the aim of the present study was to assess the effectiveness of applying grape seed extract (gse) gel in periodontal pockets for the treatment of chronic periodontitis. | 2018 | 29349878 |
| effect of the essential oils from melaleuca alternifolia, melaleuca quinquenervia and backhousia citriodora on the synthesis of ochratoxin a by aspergillus niger and aspergillus carbonarius isolated from tropical wine grapes. | the influence of essential oils (eos) extracted from the leaves of melaleuca alternifolia, melaleuca quinquenervia and backhousia citriodora on ochratoxin a (ota) synthesis by fungi was studied. the extraction of eos was performed by hydrodistillation (clevenger apparatus) over a 2-h period and subsequently analyzed by gc-ms and gc-fid. the toxigenic activity of the essential oils (31.25; 15.62 and 7.81 µg ml-1) was evaluated by inhibiting the production of ota by aspergillus niger and aspergill ... | 2018 | 29358835 |
| ectopic expression of arabidopsis broad-spectrum resistance gene rpw8.2 improves the resistance to powdery mildew in grapevine (vitis vinifera). | powdery mildew is the most economically important disease of cultivated grapevines worldwide. here, we report that the arabidopsis broad-spectrum disease resistance gene rpw8.2 could improve resistance to powdery mildew in vitis vinifera cv. thompson seedless. the rpw8.2-yfp fusion gene was stably expressed in grapevines from either the constitutive 35s promoter or the native promoter (np) of rpw8.2. the grapevine shoots and plantlets transgenic for 35s::rpw8.2-yfp showed reduced rooting and red ... | 2018 | 29362096 |
| tempranillo clones differ in the response of berry sugar and anthocyanin accumulation to elevated temperature. | the intra-varietal genetic diversity of grapevine (vitis vinifera l.) may be exploited to maintain grape quality under future warm conditions, which may alter grape berry development and composition. the present study assesses the effects of elevated temperature on the development of berry, grape composition and anthocyanins:sugars ratio of thirteen clones of v. vinifera. cv. tempranillo that differed in length of the ripening period (time from veraison to berry total soluble solids, mainly suga ... | 2018 | 29362101 |
| distribution and characterization of the vitis vinifera l. subsp. sylvestris in southern tuscany. | the eurasian grapevine is the most widespread fruit crop in the world. numerous studies have focused on clarifying the events of domestication and the geographical areas in which they occurred. | 2018 | 29366430 |
| evidence-based treatment of atopic dermatitis with topical moisturizers. | skin barrier restoration represents the mainstay of the treatment of atopic dermatitis and the use of moisturizers is recommended by several international guidelines. the aim of the study was to investigate through an evidence-based medicine analysis the effectiveness and safety of different moisturizing products available for a non-pharmacological treatment of atopic dermatitis. a total of 92 randomized controlled trials (rcts) have been identified and analyzed. the results confirm the presence ... | 2018 | 29368843 |
| recent advances in postharvest technology of the wine grape to improve the wine aroma. | postharvest techniques are widely used for the handling and storage of fresh horticultural crops. some of these crops are interesting for use with wine grapes to improve the quality of wine. in this review, we consider the postharvest techniques that are already commercially used in wine sector and others that may be significant in inducing or extracting the aroma from grapes to produce high-quality wines. | 2018 | 29369355 |
| brief challenges on medicinal plants: an eye-opening look at ageing-related disorders. | several studies have reported that nature-derived antioxidants may prevent free radicals over-production and therefore control the onset and prevent the exacerbation of different kinds of diseases caused by oxidative stress and redox-derived stressors, including ageing, fundamentally by suppressing the oxidative byproducts-mediated degradation. naturally derived antioxidants exert their anti-ageing action via a panoply of signalling systems, many of which engaging reactive oxygen (ros) and nitro ... | 2018 | 29369521 |
| quantitative assessment of grapevine wood colonization by the dieback fungus eutypa lata. | eutypa lata is a fungal pathogen causing severe dieback in vineyards worldwide. this fungus colonizes vines through pruning wounds, eventually causing a brown sectorial necrosis in wood as well as stunted vegetative growth. several years may pass between infection and the expression of external symptoms, hindering the rapid evaluation of both grapevine cultivars susceptibility and e. lata variation in aggressiveness. we aimed to develop a rapid quantitative method for the assessment of wood colo ... | 2017 | 29371539 |