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effect of maceration duration on physicochemical characteristics, organic acid, phenolic compounds and antioxidant activity of red wine from vitis vinifera l. karaoglan.effects of different maceration times (5, 10 and 15 days) on composition, phenolic compounds and antioxidant activities of red wines made from the vitis vinifera l. karaoglan grown in malatya were investigated. maceration duration changed some chemical constituents and color of karaoglan red wines. a linear relationship was observed between antioxidant activity of wine and maceration duration. major organic acid was tartaric acid which was at the highest concentration in wine macerated for 10 da ...201627777462
a draft genome sequence of pseudomonas veronii r4: a grapevine (vitis vinifera l.) root-associated strain with high biocontrol potential.a new plant commensal pseudomonas veronii isolate (strain r4) was identified from a xiphinema index biocontrol screen. isolated from grapevine roots from vineyards in central chile, the strain r4 exhibited a slower yet equivalently effective nematicide activity as the well-characterized p. protegens cha0. whole genome sequencing of strain r4 and comparative analysis among the available pseudomonas spp. genomes allowed for the identification of gene clusters that encode putative extracellular pro ...201627777646
deep analysis of wild vitis flower transcriptome reveals unexplored genome regions associated with sex specification.rna-seq of vitis during early stages of bud development, in male, female and hermaphrodite flowers, identified new loci outside of annotated gene models, suggesting their involvement in sex establishment. the molecular mechanisms responsible for flower sex specification remain unclear for most plant species. in the case of v. vinifera ssp. vinifera, it is not fully understood what determines hermaphroditism in the domesticated subspecies and male or female flowers in wild dioecious relatives (vi ...201727778293
anti-oxidant, anti-inflammatory, analgesic and antipyretic activities of grapevine leaf extract (vitis vinifera) in mice and identification of its active constituents by lc-ms/ms analyses.the leaves of vitis vinifera is used in traditional medicine for diarrhea, hepatitis and stomachaches. the objective of this study was to investigate the anti-oxidant, anti-inflammatory, analgesic and antipyretic properties of the hydroalcoholic leaf extract of vitis vinifera (evv) on experimental models to provide scientific basis for its use.201627780137
flavonoids and darkness lower pcd in senescing vitis vinifera suspension cell cultures.senescence is a key developmental process occurring during the life cycle of plants that can be induced also by environmental conditions, such as starvation and/or darkness. during senescence, strict control of genes regulates ordered degradation and dismantling events, the most remarkable of which are genetically programmed cell death (pcd) and, in most cases, an upregulation of flavonoid biosynthesis in the presence of light. flavonoids are secondary metabolites that play multiple essential ro ...201627782806
influence of physical and chemical characteristics of wine grapes on the incidence of penicillium and aspergillus fungi in grapes and ochratoxin a in wines.the incidence of filamentous fungi and toxin levels in grapes and wines varies depending on the variety of grapes, the wine region, agricultural practices, weather conditions, the harvest and the winemaking process. in this sense, the objective of this study was to evaluate the diversity of aspergillus and penicillium fungi isolated from wine grapes of the semi-arid tropical region of brazil, evaluate the presence of ochratoxin a (ota) in the experimental wine and verify if there is a correlatio ...201727792949
regulation of polar auxin transport in grapevine fruitlets (vitis vinifera l.) and the proposed role of auxin homeostasis during fruit abscission.indole-3-acetic acid (iaa), the most abundant auxin, is a growth promoter hormone involved in several developmental processes. auxin homeostasis is very important to its function and this is achieved through the regulation of iaa biosynthesis, conjugation, degradation and transport. in grapevine, iaa plays an essential role during initial stages of berry development, since it delays fruitlet abscission by reducing the ethylene sensitivity in the abscission zone. for this reason, continuous polar ...201627793088
environmental factors correlated with the metabolite profile of vitis vinifera cv. pinot noir berry skins along a european latitudinal gradient.mature berries of pinot noir grapevines were sampled across a latitudinal gradient in europe, from southern spain to central germany. our aim was to study the influence of latitude-dependent environmental factors on the metabolite composition (mainly phenolic compounds) of berry skins. solar radiation variables were positively correlated with flavonols and flavanonols and, to a lesser extent, with stilbenes and cinnamic acids. the daily means of global and erythematic uv solar radiation over lon ...201627794599
study of the terpene profile at harvest and during berry development of vitis vinifera l. aromatic varieties aleatico, brachetto, malvasia di candia aromatica and moscato bianco.in this work, we evaluated the aromatic composition of berries at harvest and during the whole berry development of aleatico, brachetto, malvasia di candia aromatica and moscato bianco grapevine varieties, which were cultivated in the same vineyard and under the same agricultural conditions.201727801497
comparative transcriptomic analysis of grape berry in response to root restriction during developmental stages.root restriction improved berry quality by being involved in diverse aspects of grapevine life. however, the molecular mechanism driving this process is not understood very well. in this study, the 'summer black' grape berry (vitis vinifera × v. labrusca) under root restriction was investigated, which showed an increase of total soluble solids (tss), color index of red grapes (cirg) value, anthocyanins accumulation, total phenolics and total procyanidins contents during berry development compare ...201627801843
the effects of pre-fermentative addition of oenological tannins on wine components and sensorial qualities of red wine.today in the wine industry, oenological tannins are widely used to improve wine quality and prevent oxidation in wine aging. with the development of tannin products, new oenological tannins are developed with many specific functions, such as modifying antioxidant effect, colour stabilization and aroma modifications. the aim of this work is to investigate effects of pre-fermentative addition of oenological tannins on wine colour, anthocyanins, volatile compounds and sensorial properties. in this ...201627809234
validation of reference genes for normalization of gene expression by qrt-pcr in a resveratrol-producing entophytic fungus (alternaria sp. mg1).alternaria sp. mg1, an endophytic fungus isolated from vitis vinifera, can independently produce resveratrol, indicating that this species contains the key genes for resveratrol biosynthesis. identification of these key genes is essential to understand the resveratrol biosynthesis pathway in this strain, which is currently unknown in microorganisms. qrt-pcr is an efficient and widely used method to identify the key genes related to unknown pathways at the level of gene expression. verification o ...201627826948
transcriptome analyses of seed development in grape hybrids reveals a possible mechanism influencing seed size.seedlessness in grape (vitis vinifera) is of considerable commercial importance for both the table grape and processing industries. studies to date of grape seed development have been made certain progress, but many key genes have yet to be identified and characterized.201627829355
the application of leaf ultrasonic resonance to vitis vinifera l. suggests the existence of a diurnal osmotic adjustment subjected to photosynthesis.the main objective of this study was to apply the air-coupled broad-band ultrasonic spectroscopy in attached transpiring leaves of vitis vinifera l. to monitor changes in leaf water potential (ψ) through the measurements of the standardized value of the resonant frequency associated with the maximum transmitance (f/fo). with this purpose, the response of grapevine to a drought stress period was investigated in terms of leaf water status, ultrasounds, gas exchange and sugar accumulation. two stro ...201627833626
gene-metabolite networks of volatile metabolism in airen and tempranillo grape cultivars revealed a distinct mechanism of aroma bouquet production.volatile compounds are the major determinants of aroma and flavor in both grapes and wine. in this study, we investigated the emission of volatile and non-volatile compounds during berry maturation in two grape varieties (airén and tempranillo) throughout 2010 and 2011. hs-spme coupled to gas chromatography and mass spectrometry was applied for the identification and relative quantitation of these compounds. principal component analysis was performed to search for variability between the two cul ...201627833635
improving respiration measurements with gas exchange analyzers.dark respiration measurements with open-flow gas exchange analyzers are often questioned for their low accuracy as their low values often reach the precision limit of the instrument. respiration was measured in five species, two hypostomatous (vitis vinifera l. and acanthus mollis) and three amphistomatous, one with similar amount of stomata in both sides (eucalyptus citriodora) and two with different stomata density (brassica oleracea and vicia faba). co2 differential (δco2) increased two-fold ...201627835767
natural product htp screening for antibacterial (e.coli 0157:h7) and anti-inflammatory agents in (lps from e. coli o111:b4) activated macrophages and microglial cells; focus on sepsis.acute systemic inflammatory response syndrome arising from infection can lead to multiple organ failure and death, with greater susceptibility occurring in immunocompromised individuals. moreover, sub-acute chronic inflammation is a contributor to the pathology of diverse degenerative diseases (parkinson's disease, alzheimer's disease and arthritis). given the known limitations in western medicine to treat a broad range of inflammatory related illness as well as the emergence of antibiotic resis ...201627846826
the synthesis and accumulation of resveratrol are associated with veraison and abscisic acid concentration in beihong (vitis vinifera × vitis amurensis) berry skin.resveratrols are polyphenolic secondary metabolites that can benefit human health, and only occur in a few plant families including vitaceae. it has been reported that abscisic acid (aba) can induce veraison (the onset of grape berry ripening) and may induce the accumulation of resveratrol in berry skin. however, the relationships between aba, veraison, the accumulation of anthocyanins and the accumulation of resveratrol in the berry are poorly understood. this study attempted to answer this que ...201627857716
factors influencing stomatal conductance in response to water availability in grapevine: a meta-analysis.the main factors regulating grapevine response to decreasing water availability were assessed under statistical support using published data related to leaf water relations in an extensive range of scion and rootstock genotypes. matching leaf water potential (ψleaf ) and stomatal conductance (gs ) data were collected from peer-reviewed literature with associated information. the resulting database contained 718 data points from 26 different vitis vinifera varieties investigated as scions, 15 non ...201727859326
crystallographic insight into the evolutionary origins of xyloglucan endotransglycosylases and endohydrolases.the xyloglucan endotransglycosylase/hydrolase (xth) gene family encodes enzymes of central importance to plant cell wall remodeling. the evolutionary history of plant xth gene products is incompletely understood vis-à-vis the larger body of bacterial endoglycanases in glycoside hydrolase family 16 (gh16). to provide molecular insight into this issue, high-resolution x-ray crystal structures and detailed enzyme kinetics of an extant transitional plant endoglucanase (eg) were determined. functiona ...201727859885
water activity and temperature effects on growth and mycotoxin production by alternaria alternata strains isolated from malbec wine grapes.to study the effects of water activity (aw ; 0·99, 0·98, 0·97, 0·96 and 0·95), temperature (15, 25 and 30°c), incubation time (7-28 days), and their interactions on mycelial growth and alternariol (aoh), alternariol monomethyl ether (ame) and tenuazonic acid (ta) mycotoxin production on a synthetic nutrient (sn) media similar to grape composition by three strains of alternaria alternata isolated from wine grapes from argentina.201727860113
the evolution of total phenolic compounds and antioxidant activities during ripening of grapes (vitis vinifera l., cv. tempranillo) grown in semiarid region: effects of cluster thinning and water deficit.a study was made of how water status (rainfed vs. irrigated) and crop load (no cluster thinning vs. cluster thinning) can together affect the grapes of vitis vinifera cv. tempranillo vines growing in a semiarid zone of extremadura (spain). the grapes were monitored at different stages of ripening, measuring the peroxidase (pox) and superoxide dismutase (sod) antioxidant activities and the phenolic content (flavonoids and phenylpropanoids), together with other parameters. the irrigation regime wa ...201627869671
chemometric analysis of minerals and trace elements in sicilian wines from two different grape cultivars.chemometric analysis are used for food authenticity evaluation, correlating botanical and geographical origins with food chemical composition. this research was carried out in order to prove that it is possible linked red wines to nero d'avola and syrah cultivars of vitis vinifera according to their mineral content, while the values of the physical and chemical parameters do not affect relevantly this discrimination. the levels of mineral elements were determined by icp-oes and icp-ms. samples f ...201727871182
a genome-wide bac-end sequence survey provides first insights into sweetpotato (ipomoea batatas (l.) lam.) genome composition.sweetpotato, ipomoea batatas (l.) lam., is an important food crop widely grown in the world. however, little is known about the genome of this species because it is a highly heterozygous hexaploid. gaining a more in-depth knowledge of sweetpotato genome is therefore necessary and imperative. in this study, the first bacterial artificial chromosome (bac) library of sweetpotato was constructed. clones from the bac library were end-sequenced and analyzed to provide genome-wide information about thi ...201627871234
monitoring of compositional changes during berry ripening in grape seed extracts of cv. sangiovese (vitis vinifera l.).seed oil and flours have been attracting the interest of researchers and industry, since they contain various bioactive components. we monitored the effects of ripening on lipids, monomeric flavan-3-ols, proanthocyanidins and tocols concentration in seed extracts from organically cultivated cv. sangiovese vines.201727873332
limited linkages of aboveground and belowground phenology: a study in grape.plant phenology influences resource utilization, carbon fluxes, and interspecific interactions. although controls on aboveground phenology have been studied to some degree, controls on root phenology are exceptionally poorly understood.201627879261
varietal dependence of glvs accumulation and lox-hpl pathway gene expression in four vitis vinifera wine grapes.variety is one of the major factors influencing grape and wine aromatic characteristics. green leaf volatiles (glvs), derived from lipoxygenase-hydroperoxides lyase (lox-hpl) pathway, are important components for the aromatic quality of grapes and wines. however, the varietal difference regarding glvs accumulation and related gene expression are poorly studied. this work exhibited that the accumulation of various glvs and the expression of lox-hpl pathway genes in four vitis vinifera wine grape ...201627886056
polyphenolic diversity and characterization in the red-purple berries of east asian wild vitis species.grapes (vitis spp.) produce diverse polyphenolic compounds, which are phytochemicals that contribute to human health. in this study, the polyphenolic profiles of the red-purple berries of two wild grape species native to japan, vitis ficifolia and v. coignetiae, and their interspecific hybrid cultivars were investigated and compared with the profiles of v. vinifera and v. × labruscana cultivars. proanthocyanidins (pas) were present at lower concentrations in both skins and seeds of wild grape sp ...201727887737
spectroscopic and e-tongue evaluation of medicinal plants: a taste of how rasa can be studied.the use of medicinal plants in ayurveda is based on rasa, generally taken to represent taste as a sensory perception. this chemosensory parameter plays an important role in ayurvedic pharmacology.201727889428
fungal endophytic communities associated to the phyllosphere of grapevine cultivars under different types of management.fungal endophytes present in different asymptomatic grapevine plants (vitis vinifera l.) located in different vineyards within alentejo, a highly important viticulture region in portugal, were identified in this study. sampled grapevine plants included the three most representative cultivars in the region, syrah, cabernet sauvignon, and aragonez, growing under two different modes of management, conventional and biological. sixteen fungal taxa were identified through sequencing of the internal tr ...201627890088
anti-acne activity of italian medicinal plants used for skin infection.propionibacterium acnes is implicated in the pathogenesis of acne vulgaris, which impacts >85% of teenagers. novel therapies are in high demand and an ethnopharmacological approach to discovering new plant sources of anti-acne therapeutics could contribute to filling this void in effective therapies. the aims of our study were two-fold: (1) to determine if species identified in ethnopharmacological field studies as having traditional uses for skin and soft tissue infection (ssti) exhibit signifi ...201627891094
is there nothing new under the sun? the influence of herbals and pharmacopoeias on ethnobotanical traditions in albacete (spain).this paper has two overarching aims: (1) presenting the results of studying the albacete tariff of medicines of 1526 and (2) broadly analyzing the origin and influences of medicinal traditional knowledge in the region of albacete, spain. we use historical and modern literature that may have influenced this knowledge. our primary goal was to determine the ingredients used in the pharmacy in the 16th century ce in albacete through the analysis of the tariff, and our secondary goal was to investiga ...201727894973
grape ripening is regulated by deficit irrigation/elevated temperatures according to cluster position in the canopy.the impact of water deficit on berry quality has been extensively investigated during the last decades. nonetheless, there is a scarcity of knowledge on the performance of varieties exposed to a combination of high temperatures/water stress during the growing season and under vineyard conditions. the objective of this research was to investigate the effects of two irrigation regimes, sustained deficit irrigation (sdi, 30% etc) and regulated deficit irrigation (rdi, 15% etc) and of two cluster po ...201627895648
estimating leaf area index (lai) in vineyards using the pocketlai smart-app.estimating leaf area index (lai) of vitis vinifera using indirect methods involves some critical issues, related to its discontinuous and non-homogeneous canopy. this study evaluates the smart app pocketlai and hemispherical photography in vineyards against destructive lai measurements. data were collected during six surveys in an experimental site characterized by a high level of heterogeneity among plants, allowing us to explore a wide range of lai values. during the last survey, the possibili ...201627898028
mecs-vine®: a new proximal sensor for segmented mapping of vigor and yield parameters on vineyard rows.ground-based proximal sensing of vineyard features is gaining interest due to its ability to serve in even quite small plots with the advantage of being conducted concurrently with normal vineyard practices (i.e., spraying, pruning or soil tilling) with no dependence upon weather conditions, external services or law-imposed limitations. the purpose of the present work was to test performance of the new terrestrial multi-sensor mecs-vine® in terms of reliability and degree of correlation with sev ...201627898049
overexpression of a thaumatin-like protein gene from vitis amurensis improves downy mildew resistance in vitis vinifera grapevine.downy mildew is a highly destructive disease in grapevine production. a gene encoding pathogenesis-related (pr) thaumatin-like protein was isolated from the downy mildew-resistant grapevine "zuoshan-1," a clonal selection from wild vitis amurensis rupr. the predicted thaumatin-like protein (vatlp) has 225 amino acids and it is acidic, with a calculated isoelectric point of 4.8. the full length of the vatlp gene was transformed into somatic embryogenic calli of v. vinifera 'thompson seedless' via ...201627900595
phenolic and aroma composition of white wines produced by prolonged maceration and maturation in wooden barrels.to investigate the phenolic and aroma composition of malvazija istarska (vitis vinifera l.) white wines produced by an unconventional technology comprising prolonged maceration followed by maturation in wooden barrels, representative samples were subjected to analysis by uv/vis spectrometry, high-performance liquid chromatography, and gas chromatography-mass spectrometry. when compared to standard wines, the investigated samples contained higher levels of dry extract, volatile acidity, lactic ac ...201527904375
broad-spectrum of antimicrobial properties of commercial wines from different vitis vinifera l. varieties.fifteen commercial wines produced from international and autochthonic varieties of vitis vinifera l. cultivation of different balkan winegrowing subregions were studied for their antimicrobial activity against six gram-positive (clostridium perfringens, bacillus subtilis, staphylococcus aureus, listeria inocua, sarcina lutea and micrococcus flavus) and six gram-negative (escherichia coli, pseudomonas aeruginosa, salmonella enteritidis, shigella sonnei, klebsiella pneumonia and proteus vulgaris) ...201727905092
genome-wide characterisation and expression profile of the grapevine atl ubiquitin ligase family reveal biotic and abiotic stress-responsive and development-related members.the arabidopsis tóxicos en levadura (atl) protein family is a class of e3 ubiquitin ligases with a characteristic ring-h2 zn-finger structure that mediates diverse physiological processes and stress responses in plants. we carried out a genome-wide survey of grapevine (vitis vinifera l.) atl genes and retrieved 96 sequences containing the canonical atl ring-h2 domain. we analysed their genomic organisation, gene structure and evolution, protein domains and phylogenetic relationships. clustering ...201627910910
morphology and hydraulic architecture of vitis vinifera l. cv. syrah and torrontés riojano plants are unaffected by variations in red to far-red ratio.plants have evolved an array of specific photoreceptors to acclimate to the light environment. by sensing light signals, photoreceptors modulate plant morphology, carbon- and water-physiology, crop yield and quality of harvestable organs, among other responses. many cultural practices and crop management decisions alter light quantity and quality perceived by plants cultivated in the field. under full sunlight, phytochromes perceive high red to far red ratios (r:fr; 1.1), whereas overhead or lat ...201627911923
proteins immunologically related to map65-1 accumulate and localize differentially during bud development in vitis vinifera l.various arrays of microtubules are present throughout the plant cell cycle and are involved in distinct functions. microtubule-associated proteins (maps) regulate microtubule dynamics by acting as stabilizers, destabilizers, and promoters of microtubule dynamics. the map65 family is a specific group of cross-linkers required for structural maintenance of microtubules. in plants, different isoforms of map65 are differentially expressed according to their developmental program. in this work, we an ...201727913905
multiplex detection of aspergillus species.multiplex real-time polymerase chain reaction (pcr) provides a fast and accurate dna-based tool for the simultaneous amplification of more than one target sequence in a single reaction. here a duplex real-time pcr assay is described for the simultaneous detection of aspergillus carbonarius and members of the aspergillus niger aggregate, which are the main responsible species for ochratoxin a (ota) contamination in grapes. this single tube reaction targets the beta-ketosynthase and the acyl trans ...201727924544
biostability of the proanthocyanidins-dentin complex and adhesion studies.oligomeric proanthocyanidins (opacs) are potent and renewable natural bioactives possible to be refined into chemically standardized mixtures for biological applications. herein, we found that multiscale interactions of opacs with the dentin matrix create tight biointerfaces with hydrophobic methacrylate adhesives on wet surfaces. an enriched mixture of opacs, with a known phytochemical profile, was produced from grape seed crude extract ( vitis vinifera; enriched grape seed extract [e-gse]) and ...201727927886
comparative genomic analysis of the grf genes in chinese pear (pyrus bretschneideri rehd), poplar (populous), grape (vitis vinifera), arabidopsis and rice (oryza sativa).growth-regulating factors (grfs) are plant-specific transcription factors that have important functions in regulating plant growth and development. previous studies on grf family members focused either on a single or a small set of genes. here, a comparative genomic analysis of the grf gene family was performed in poplar (a model tree species), arabidopsis (a model plant for annual herbaceous dicots), grape (one model plant for perennial dicots), rice (a model plant for monocots) and chinese pea ...201627933074
revisiting vitis vinifera subtilase gene family: a possible role in grapevine resistance against plasmopara viticola.subtilisin-like proteases, also known as subtilases, are a very diverse family of serine peptidases present in many organisms. in grapevine, there are hints of the involvement of subtilases in defense mechanisms, but their role is not yet understood. the first characterization of the subtilase gene family was performed in 2014. however, simultaneously, the grapevine genome was re-annotated and several sequences were re-annotated or retrieved. we have performed a re-characterization of this famil ...201627933087
inhibitory activity of beauveria bassiana and trichoderma spp. on the insect pests xylotrechus arvicola (coleoptera: cerambycidae) and acanthoscelides obtectus (coleoptera: chrisomelidae: bruchinae).xylotrechus arvicola is an important pest in vineyards (vitis vinifera) in the main iberian wine-producing regions, and acanthoscelides obtectus causes severe post-harvest losses in the common bean (phaseolus vulgaris). under laboratory conditions with a spray tower, the susceptibility of the immature stages of x. arvicola and a. obtectus against the entomopathogenic fungi beauveria bassiana and four strains of trichoderma spp. was evaluated. both insect pests t. harzianum and b. bassiana showed ...201727933578
13c nmr and lc-ms profiling of stilbenes from elicited grapevine hairy root cultures.resveratrol and related oligostilbenes are defense molecules produced by grapevine in response to stresses including various elicitors or signal molecules. together with their prominent role in planta, these compounds have been the center of much attention in recent decades due to their pharmacological properties. the cost-effective production of resveratrol derivatives such as viniferins or more structurally complex stilbene oligomers remains a challenging task. in this study, the chemical dive ...201627933900
structural and biochemical characterization of cinnamoyl-coa reductases.cinnamoyl-coenzyme a reductase (ccr) catalyzes the reduction of hydroxycinnamoyl-coenzyme a (coa) esters using nadph to produce hydroxycinnamyl aldehyde precursors in lignin synthesis. the catalytic mechanism and substrate specificity of cinnamoyl-coa reductases from sorghum (sorghum bicolor), a strategic plant for bioenergy production, were deduced from crystal structures, site-directed mutagenesis, and kinetic and thermodynamic analyses. although sbccr1 displayed higher affinity for caffeoyl-c ...201727956488
expression profiles and dna-binding affinity of five erf genes in bunches of vitis vinifera cv. cardinal treated with high levels of co2 at low temperature.ethylene response factors (erfs) play an important role in plants by regulating defense response through interaction with various stress pathways. after harvest, table grapes (vitis vinifera l.) are subject to a range of problems associated with postharvest storage at 0°c, such as fungal attack, water loss and rachis browning. the application of a 3-day high co2 treatment maintained fruit quality and activated the induction of transcription factors belonging to different families such as erf. in ...201627965678
contrasting susceptibilities to flavescence dorée in vitis vinifera, rootstocks and wild vitis species.flavescence dorée (fd) is a quarantine disease of grapevine, involving interactions between the plants, leafhopper vectors, and fd phytoplasma. characterizing the susceptibility of vine varieties could limit disease propagation. after extensive surveys in vineyards, we showed that cabernet sauvignon (cs) is highly susceptible, with a high proportion of symptomatic branches and phytoplasma titers, in contrast to merlot (m). localized insect transmissions and grafting showed that phytoplasma circu ...201627965681
myba2 gene involved in anthocyanin and flavonol biosynthesis pathways in grapevine.myba2 transcription factor (myb-related gene) affects the coloring in grapevine berry and plays an important role in the biosynthesis of anthocyanin. the myba2 gene was cloned from vitis vinifera l. cv. cabernet sauvignon and polyclonal antibodies for vvmyba2 were prepared. the vvmyba2 gene expression patterns were observed in seven tissues (the leaf, stem, flower, bud, root, berry, and tendril) and during the berry development stage at transcriptional and translational levels, respectively. the ...201627966737
enhanced stilbene production and excretion in vitis vinifera cv pinot noir hairy root cultures.stilbenes are defense molecules produced by grapevine in response to stresses including various elicitors and signal molecules. together with their prominent role in planta, stilbenes have been the center of much attention in recent decades due to their pharmaceutical properties. with the aim of setting up a cost-effective and high purity production of resveratrol derivatives, hairy root lines were established from vitis vinifera cv pinot noir 40024 to study the organ-specific production of vari ...201627973421
potassium uptake and redistribution in cabernet sauvignon and syrah grape tissues and its relationship with grape quality parameters.the present study investigated the potassium (k) levels in petiole and other grape tissues during ripening in vitis vinifera shiraz and cabernet sauvignon, grown in areas with differences in vigour, as well as with and without leaf thinning. potassium levels in petiole, seeds, skin and flesh were related to grape ph, acidity, berry weight and total soluble solids.201727976407
cooking impact in color, pigments and volatile composition of grapevine leaves (vitis vinifera l. var. malvasia fina and touriga franca).grapevine leaves (vitis vinifera l. var. malvasia fina and touriga franca) under culinary treatment (blanching and boiling at 60, 75 and 90min) were studied for their color, pigments and volatile fraction changes. blanching and boiling caused a decrease in luminosity and a loss of green coloration in both varieties, while a yellow-brownish color arose. significant correlations were established between the loss of green color (monochromatic variable a∗) and the total chlorophylls content. the mai ...201727979078
distribution of yloid in soil-grapevine system (vitis vinifera l.) as tool for geographical characterization of agro-food products. a two years case study on different grafting combinations.the knowledge of a chemistry relationship between the soil and the agricultural products is an important tool for the quality assessment of food. we studied yloid (y, la and lanthanoids), recognized as very useful tracers due their coherent and predictable behavior, to trace and evaluate their distribution from soil to the grape in vitis vinifera l. because much of the world's viticulture is based on grafting, and rootstocks have proved affect vine growth, yield, fruit and wine quality, we carri ...201727979080
evaluation of the influence of white grape seed extracts as copigment sources on the anthocyanin extraction from grape skins previously classified by near infrared hyperspectral tools.hyperspectral imaging has been used to classify red grapes (vitis vinifera l.) according to their predicted extractable total anthocyanin content (i.e. extractable total anthocyanin content determined by a hyperspectral method). low, medium and high levels of predicted extractable total anthocyanin content were established. then, grape skins were split into three parts and each part was macerated into a different model wine solution for a three-day period. wine model solutions were made up with ...201727979147
potential health benefits and quality of dried fruits: goji fruits, cranberries and raisins.dried fruits are important snacks and additives to other foods due to their taste and nutritional advantages. therefore there is an important goal to control the quality of the food on the market for consumer's safety. antioxidant activity of goji fruits (lycium barbarum), cranberries (vaccinium macrocarpon and oxycoccus) and raisins (vitis vinifera) were studied using the dpph (2,2-diphenyl-1-picrylhydrazyl) and folin-ciocalteu assays. cu, mn and ge influencing antioxidant activity were determi ...027979197
sun dried corinthian currant (vitis vinifera l., var. apyrena) simple sugar profile and macronutrient characterization.corinthian currant simple sugar profile was determined by high-pressure liquid chromatography coupled with refractive index detection; the content in other macroconstituents was also assessed. corinthian currants analyzed were obtained over three consecutive crop years, while the effect of cultivation area altitude was also evaluated. sugar extraction was optimized with respect to solvent, process, and time. simple sugar chromatographic analysis was validated for linearity (r2>0.999), limits of ...201727979215
temperature and co2 dependency of the photosynthetic photon flux density responses of leaves of vitis vinifera cvs. chardonnay and merlot grown in a hot climate.comparisons of the photosynthetic responses to light and temperature between related cultivars are important to understand how well matched they are to the climate where they are grown. photosynthetic light responses at a range of leaf temperatures and two co2 concentrations were measured on leaves of two grapevine cultivars (vitis vinifera l.) chardonnay and merlot vines growing in field conditions. the objective was to assess the interaction between photon flux density (pfd), leaf temperature ...201727987474
specific adjustments in grapevine leaf proteome discriminating resistant and susceptible grapevine genotypes to plasmopara viticola.grapevine downy mildew is an important disease affecting crop production leading to severe yield losses. this study aims to identify the grapevine cultivar-specific adjustments of leaf proteome that allow the discrimination between resistance and susceptibility towards p. viticola (constitutive (0h) and in after inoculation (6, 12 and 24h). leaf proteome analysis was performed using 2d difference gel electrophoresis followed by protein identification via mass spectrometry. in addition, we analys ...201727989945
aba and ga3 regulate the synthesis of primary and secondary metabolites related to alleviation from biotic and abiotic stresses in grapevine.plants are able to synthesize a large number of organic compounds. among them, primary metabolites are known to participate in plant growth and development, whereas secondary metabolites are mostly involved in defense and other facultative processes. in grapevine, one of the major fruit crops in the world, secondary metabolites, mainly polyphenols, are of great interest for the wine industry. even though there is an extensive literature on the content and profile of those compounds in berries, s ...027998613
resveratrol as an anticancer agent: a review.owing to their antimicrobial, antioxidant, and anti-inflammatory activity, grapes (vitis vinifera l.) are the archetypal paradigms of fruits used not only for nutritional purposes but also for exclusive therapeutics. grapes are a prominent and promising source of phytochemicals, especially resveratrol, a phytoalexin antioxidant found in red grapes which has both chemopreventive and therapeutic effects against various ailments. resveratrol's role in reducing different human cancers, including bre ...028001084
catchup: a web database for spatiotemporally regulated genes.for proper control of biological activity, some key genes are highly expressed in a particular spatiotemporal domain. mining of such spatiotemporally expressed genes using large-scale gene expression data derived from a broad range of experimental sources facilitates our understanding of genome-scale functional gene networks. however, comprehensive information on spatiotemporally expressed genes is lacking in plants. to collect such information, we devised a new index, δdmax, which is the maximu ...201728013273
insights into the role of the berry-specific ethylene responsive factor vvierf045.during grape ripening, numerous transcriptional and metabolic changes are required in order to obtain colored, sweet, and flavored berries. there is evidence that ethylene, together with other signals, plays an important role in triggering the onset of ripening. here, we report the functional characterization of a berry-specific ethylene responsive factor (erf), vvierf045, which is induced just before véraison and peaks at ripening. phylogenetic analysis revealed it is close to the shine clade o ...201628018369
differential expression of mirnas and associated gene targets in grapevine leafroll-associated virus 3-infected plants.micrornas (mirnas) are a class of endogenous small non-coding rnas (srna) that play an essential role in the regulation of target mrnas expressed during plant development and in response to stress. microrna expression profiling has helped to identify mirnas that regulate a range of processes, including the plant's defence response to pathogens. in this study, differential mirna expression in own-rooted vitis vinifera cv. cabernet sauvignon plants infected with grapevine leafroll-associated virus ...201728025711
htp nutraceutical screening for histone deacetylase inhibitors and effects of hdacis on tumor-suppressing mirnas by trichostatin a and grapeseed (vitis vinifera) in hela cells.aggressive tumor malignancies are a consequence of delayed diagnosis, epigenetic/phenotype changes and chemo-radiation resistance. histone deacetylases (hdacs) are a major epigenetic regulator of transcriptional repression, which are highly overexpressed in advanced malignancy. while original chemotherapy drugs were modeled after phytochemicals elucidated by botanical screenings, hdac inhibitors (hdaci) such as apicidin, trichostatin a (tsa) and butyrate were discovered as products of fungus and ...201728031235
impact of different types of stoppers on sensorial and phenolic characteristics evolution during a bottle storage time of a white wine from chardonnay grape variety.the objective of this work was to study the correlation between the variation of phenolic compounds and sensory characteristics in white wine during bottle storage and to explore the compounds that affected sensory evolution. chardonnay (vitis vinifera l. cv.) dry white wines were bottled under six types of stoppers and stored for 18 months. the composition of phenolic compounds was analyzed, and the sensory attributes of these wines were evaluated by professional panel. multivariate statistical ...201628035160
differential phenolic production in leaves of vitis vinifera cv. alvarinho affected with esca disease.esca is a destructive disease of complex etiology affecting grapevines worldwide. a major constraint to the study and control of esca is that the disease is not diagnosed until external leaf and/or fruit symptoms are visible; however external symptoms usually appear several years after infection onset. we studied the phenolic content of v. vinifera cv. alvarinho leaves using high performance liquid chromatography-diode array detection-mass spectrometry (hplc-dad-ms)/lc-ms. leaves from affected c ...201728039815
overexpression of a stress-responsive u-box protein gene vapub affects the accumulation of resistance related proteins in vitis vinifera 'thompson seedless'.many u-box proteins have been identified and characterized as important factors against environmental stresses such as chilling, heat, salinity and pathogen attack in plant. our previous research reported the cloning of a novel u-box protein gene vapub from vitis amurensis 'zuoshanyi' grape and suggested a function of it in related to cold stress in the model plant arabidopsis system. in this study, the role of vapub in response to biotic and abiotic stress was further analyzed in the homologous ...201728039816
integrating omics and alternative splicing reveals insights into grape response to high temperature.heat stress is one of the primary abiotic stresses that limit crop production. grape (vitis vinifera) is a cultivated fruit with high economic value throughout the world, with its growth and development often influenced by high temperature. alternative splicing (as) is a widespread phenomenon increasing transcriptome and proteome diversity. we conducted high-temperature treatments (35°c, 40°c, and 45°c) on grapevines and assessed transcriptomic (especially as) and proteomic changes in leaves. we ...201728049741
reduction of heterozygosity (roh) as a method to detect mosaic structural variation. 201728055146
comparative effect of grape seed extract (vitis vinifera) and ascorbic acid in oxidative stress induced by on-pump coronary artery bypass surgery.this study aimed to test the beneficial effect of grape seed extract (gse) (vitis vinifera) and vitamin c in oxidative stress and reperfusion injury induced by cardiopulmonary bypass (cpb) in coronary artery bypass surgery.201728074795
association between grape yeast communities and the vineyard ecosystems.the grape yeast biota from several wine-producing areas, with distinct soil types and grapevine training systems, was assessed on five islands of azores archipelago, and differences in yeast communities composition associated with the geographic origin of the grapes were explored. fifty-seven grape samples belonging to the vitis vinifera grapevine cultivars verdelho dos açores (verdelho), arinto da terceira (arinto) and terrantez do pico (terrantez) were collected in two consecutive years and 40 ...201728085916
an inert pesticide adjuvant synergizes viral pathogenicity and mortality in honey bee larvae.honey bees are highly valued for their pollination services in agricultural settings, and recent declines in managed populations have caused concern. colony losses following a major pollination event in the united states, almond pollination, have been characterized by brood mortality with specific symptoms, followed by eventual colony loss weeks later. in this study, we demonstrate that these symptoms can be produced by chronically exposing brood to both an organosilicone surfactant adjuvant (os ...201728091574
preclinical and potential applications of common western herbal supplements as complementary treatment in parkinson's disease.parkinson's disease (pd) is a neurological disorder with a complex pathological etiology, which is not fully understood. progression of pd may be the result of a buildup of iron in the substantia nigra, microglia-mediated neuroinflammation, dysfunctional mitochondria, or abnormal protein handling. dopamine is the main neurotransmitter affected, but as the disease progresses, a decrease in all the brain's biogenic amines occurs. current medication used in the treatment of pd aims to prevent the b ...201728095073
crispri-mediated metabolic engineering of e. coli for o-methylated anthocyanin production.anthocyanins are a class of brightly colored, glycosylated flavonoid pigments that imbue their flower and fruit host tissues with hues of predominantly red, orange, purple, and blue. although all anthocyanins exhibit ph-responsive photochemical changes, distinct structural decorations on the core anthocyanin skeleton also cause dramatic color shifts, in addition to improved stabilities and unique pharmacological properties. in this work, we report for the first time the extension of the reconsti ...201728095853
transcriptome analysis of genes involved in anthocyanins biosynthesis and transport in berries of black and white spine grapes (vitis davidii).the color of berry skin is an important economic trait for grape and is essentially determined by the components and content of anthocyanins. the fruit color of chinese wild grapes is generally black, and the profile of anthocyanins in chinese wild grapes is significantly different from that of vitis vinifera. however, v. davidii is the only species that possesses white berry varieties among chinese wild grape species. thus, we performed a transcriptomic analysis to compare the difference of tra ...201628096779
comparative rna-seq based transcriptomic analysis of bud dormancy in grape.bud dormancy is an important biological phenomenon of perennial plants that enables them to survive under harsh environmental circumstances. grape (vitis vinifera) is one of the most grown fruit crop worldwide; however, underlying mechanisms involved in grape bud dormancy are not yet clear. this work was aimed to explore the underlying molecular mechanism regulating bud dormancy in grape.201728103799
a grapevine ttg2-like wrky transcription factor is involved in regulating vacuolar transport and flavonoid biosynthesis.a small set of ttg2-like homolog proteins from different species belonging to the wrky family of transcription factors were shown to share a similar mechanism of action and to control partially conserved biochemical/developmental processes in their native species. in particular, by activating p-atpases residing on the tonoplast, ph3 from petunia hybrida promotes vacuolar acidification in petal epidermal cells whereas ttg2 from arabidopsis thaliana enables the accumulation of proanthocyanidins in ...201628105033
phytotoxic lipophilic metabolites produced by grapevine strains of lasiodiplodia species in brazil.phytotoxic metabolites produced in liquid culture by six species of lasiodiplodia isolated in brazil and causing botryosphaeria dieback of grapevine were chemically identified. as ascertained by lc/ms, l. brasiliense, l. crassispora, l. jatrophicola, and l. pseudotheobromae produced jasmonic acid, and l. brasiliense synthesized, besides jasmonic acid, also (3r,4s)-4-hydroxymellein. l. euphorbicola and l. hormozganensis produced some low molecular weight lipophilic toxins. specifically, l. euphor ...201728110532
space-time point pattern analysis of flavescence dorée epidemic in a grapevine field: disease progression and recovery.analyses of space-time statistical features of a flavescence dorée (fd) epidemic in vitis vinifera plants are presented. fd spread was surveyed from 2011 to 2015 in a vineyard of 17,500 m2 surface area in the piemonte region, italy; count and position of symptomatic plants were used to test the hypothesis of epidemic complete spatial randomness and isotropicity in the space-time static (year-by-year) point pattern measure. space-time dynamic (year-to-year) point pattern analyses were applied to ...201628111581
rna-sequencing reveals biological networks during table grapevine ('fujiminori') fruit development.grapevine berry development is a complex and genetically controlled process, with many morphological, biochemical and physiological changes occurring during the maturation process. research carried out on grapevine berry development has been mainly concerned with wine grape, while barely focusing on table grape. 'fujiminori' is an important table grapevine cultivar, which is cultivated in most provinces of china. in order to uncover the dynamic networks involved in anthocyanin biosynthesis, cell ...028118385
genome-wide analysis of cis-regulatory element structure and discovery of motif-driven gene co-expression networks in grapevine.coordinated transcriptional and metabolic reprogramming ensures a plant's continued growth and survival under adverse environmental conditions. transcription factors (tfs) act to modulate gene expression through complex cis-regulatory element (cre) interactions. genome-wide analysis of known plant cres was performed for all currently predicted protein-coding gene promoters in grapevine (vitis vinifera l.). many cres such as abscisic acid (aba)-responsive, drought-responsive, auxin-responsive, an ...201728119334
endophytic bacterium pseudomonas fluorescens rg11 may transform tryptophan to melatonin and promote endogenous melatonin levels in the roots of four grape cultivars.endophytes have been verified to synthesize melatonin in vitro and promote abiotic stress-induced production of endogenous melatonin in grape (vitis vinifera l.) roots. this study aimed to further characterize the biotransformation of tryptophan to melatonin in the endophytic bacterium pseudomonas fluorescens rg11 and to investigate its capacity for enhancing endogenous melatonin levels in the roots of different grape cultivars. using ultra performance liquid chromatography-tandem mass spectrome ...201628119731
snp-discovery by rad-sequencing in a germplasm collection of wild and cultivated grapevines (v. vinifera l.).whole-genome comparisons of vitis vinifera subsp. sativa and v. vinifera subsp. sylvestris are expected to provide a better estimate of the valuable genetic diversity still present in grapevine, and help to reconstruct the evolutionary history of a major crop worldwide. to this aim, the increase of molecular marker density across the grapevine genome is fundamental. here we describe the snp discovery in a grapevine germplasm collection of 51 cultivars and 44 wild accessions through a novel proto ...201728125640
a comparative study of the phenolic and technological maturities of red grapes grown in lebanon.grape harvest date is determined according to the technological and phenolic maturities. these parameters were calculated for different red grape (vitis vinifera l.) varieties (cabernet sauvignon, merlot, syrah, cabernet franc) over four years (2008, 2009, 2010, and 2011) (642 samples). titratable acidity and sugar content of the grapes were used to determine the technological maturity, whereas glories (1 and 2) and itv (institut technique de la vigne et du vin) methods were used to monitor thei ...201728134785
exploring the effects of geographical origin on the chemical composition and quality grading of vitis vinifera l. cv. chardonnay grapes.the relationship between berry chemical composition, region of origin and quality grade was investigated for chardonnay grapes sourced from vineyards located in seven south australian geographical indications (gi). measurements of basic chemical parameters, amino acids, elements, and free and bound volatiles were conducted for grapes collected during 2015 and 2016. multiple factor analysis (mfa) was used to determine the sets of data that best discriminated each gi and quality grade. important c ...201728146133
proteomics towards the understanding of elicitor induced resistance of grapevine against downy mildew.elicitors are known to trigger plant defenses in response to biotic stress, but do not systematically lead to effective resistance to pathogens. the reasons explaining such differences remain misunderstood. therefore, elicitation and induced resistance (ir) were investigated through the comparison of two modified β-1,3 glucans applied on grapevine (vitis vinifera) leaves before and after inoculation with plasmopara viticola, the causal agent of downy mildew. the sulfated (ps3) and the shortened ...201728153682
flavanol quantification of grapes via multiple reaction monitoring mass spectrometry. application to differentiation among clones of vitis vinifera l. cv. rufete grapes.the determination of the detailed flavanol composition in food matrices is not a simple task because of the structural similarities of monomers and, consequently, oligomers and polymers. the aim of this study was the development and validation of an hplc-ms/ms-multiple reaction monitoring (mrm) method that would allow the accurate and precise quantification of catechins, gallocatechins, and oligomeric proanthocyanidins. the high correlation coefficients of the calibration curves (>0.993), the re ...201728158946
analysis of grapevine rupestris stem pitting-associated virus in slovakia reveals differences in intra-host population diversity and naturally occurring recombination events.grapevine rupestris stem pitting-associated virus (grspav) is a worldwide-distributed pathogen in grapevines with a high genetic variability. our study revealed differences in the complexity of grspav population in a single host. a single-variant grspav infection was detected from the sk30 grapevine plant. on the contrary, sk704 grapevine was infected by three different grspav variants. variant-specific rt-pcr detection protocols have been developed in this work to study distribution of the thre ...201728167886
preventative disease management and grower decision making: a case study of california wine-grape growers.preventative disease management is challenging to farmers because it requires paying immediate costs in the hopes of returning uncertain future benefits. understanding farmer decision making about prevention has the potential to reduce disease incidence and minimize the need for more costly postinfection practices. for example, the grapevine trunk-disease complex (esca, botryosphaeria dieback, eutypa dieback, and phomopsis dieback) significantly affects vineyard productivity and longevity. given ...201728168929
transcriptomic analyses of ascorbic acid and carotenoid metabolites influenced by root restriction during grape berry development and ripening.ascorbic acid (asa) and carotenoids are recognized as crucial metabolites for various biological processes in plants. the contents of asa and carotenoids in fruits are influenced by external environmental stimuli, such as water, temperature, light, and hormones. however, it is still not clear whether it can be affected by root restriction (rr) treatment. in this study, "summer black" grape berries (vitis vinifera × v. labrusca) under rr and control treatments during development and ripening were ...028177240
changes in the proteome of grapevine leaves (vitis vinifera l.) during long-term drought stress.the essence of exploring and understanding mechanisms of plant adaptation to environmental stresses lies in the determination of patterns of the expression of proteins, identification of stress proteins and their association with the specific functions in metabolic pathways. to date, little information has been provided about the proteomic response of grapevine to the persistent influence of adverse environmental conditions. this article describes changes in the profile of protein accumulation i ...201728178572
identification and organoleptic contribution of (z)-1,5-octadien-3-one to the flavor of vitis vinifera cv. merlot and cabernet sauvignon musts.the main goal of this research was to identify key aroma compounds involved in the dried fruits (prune and dried fig) aroma of musts. an odoriferous zone (oz) was detected by gas chromatography coupled with olfactometry (gc-o) and identified as (z)-1,5-octadien-3-one (geranium). a quantitation method by spme-gc-ms (ci, meoh) was developed and validated for assaying this very fragrant ketone in musts for the first time (lod, 0.15 ng/l; loq, 0.5 ng/l). concentrations of (z)-1,5-octadien-3-one in m ...028190353
proanthocyanidins from vitis vinifera inhibit oxidative stress-induced vascular impairment in pulmonary arteries from diabetic rats.vitis vinifera l. (grape seed extract) is a natural source of proanthocyanidins with antioxidant and free radical-scavenging activities.201728190469
the composition of cell walls from grape skin in vitis vinifera intraspecific hybrids.monastrell is a red grape cultivar adapted to the dry environmental conditions of murcia, se spain. its berries seem to be characterized by a rigid cell wall structure, which could make difficult the winemaking process. cabernet sauvignon cultivar is used to complement monastrell wines in this region owing to its high phenolic content with high extractability. this study explores the skin cell wall composition of grapes from plants resulting from intraspecific crosses of vitis vinifera cultivars ...201728195329
vviaprt3 and vvifsex: two genes involved in sex specification able to distinguish different flower types in vitis.vitis vinifera vinifera is a hermaphrodite subspecies, while its ancestor, vitis vinifera sylvestris, is dioecious. we have identified two genes that together allow the discrimination between male, female and hermaphrodite vitis plants. the sex locus region on chromosome 2 was screened resulting in the discovery of a new gene, vvifsex. the same screening revealed another gene, vviaprt3, located in the sex region, that be used as a sex marker. both genes are good candidates to be involved in flow ...201728197167
cocoa and grape seed byproducts as a source of antioxidant and anti-inflammatory proanthocyanidins.phenolic compounds, which are secondary plant metabolites, are considered an integral part of the human diet. physiological properties of dietary polyphenols have come to the attention in recent years. especially, proanthocyanidins (ranging from dimers to decamers) have demonstrated potential interactions with biological systems, such as antiviral, antibacterial, molluscicidal, enzyme-inhibiting, antioxidant, and radical-scavenging properties. agroindustry produces a considerable amount of pheno ...201728208630
changes in sugar content and related enzyme activities in table grape (vitis vinifera l.) in response to foliar selenium fertilizer.spraying selenium (se) fertilizer is an effective method for se-enriched fruit production. sugar content in fruit is the major factor determining berry quality. however, changes in sugar metabolism in response to se fertilizer are unclear. hence, this study was conducted to identify the effects of se fertilizer on sugar metabolism and related enzyme activities of grape berries. additionally, production of leaves with and without se fertilizer was also investigated.201728211621
a new strategy to enhance the biosynthesis of trans-resveratrol by overexpressing stilbene synthase gene in elicited vitis vinifera cell cultures.in this work, transgenic lines of suspension cultured cells of vitis vinifera cv. monastrell containing the plasmid pmog800-sts have been obtained. the cell growth of these transgenic cell lines decreased slightly as compared to non-transgenic suspension cultured cells, while cell viability was not affected. in addition, the elicitation with cyclodextrins and methyl jasmonate enhanced the production of trans-resveratrol, observing the highest levels of this compound in sts-expressing transgenic ...201728214727
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