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cadophora species associated with wood-decay of grapevine in north america.cadophora species are reported from grapevine (vitis vinifera l.) in california, south africa, spain, uruguay, and canada. frequent isolation from vines co-infected with the esca pathogens (togninia minima and phaeomoniella chlamydospora), and confirmation of its ability to cause wood lesions/discoloration in pathogenicity tests, suggest that c. luteo-olivacea is part of the trunk pathogen complex. in north america, little is known regarding the diversity, geographic distribution, and roles of c ...201525601149
severe drought stress is affecting selected primary metabolites, polyphenols, and volatile metabolites in grapevine leaves (vitis vinifera cv. pinot noir).extreme weather conditions with prolonged dry periods and high temperatures as well as heavy rain events can severely influence grapevine physiology and grape quality. the present study evaluates the effects of severe drought stress on selected primary metabolites, polyphenols and volatile metabolites in grapevine leaves. among the 11 primary metabolites, 13 polyphenols and 95 volatiles which were analyzed, a significant discrimination between control and stressed plants of 7 primary metabolites ...201525602440
reversal of radiocontrast medium toxicity in human renal proximal tubular cells by white grape juice extract.radiocontrast media (rcm)-induced nephrotoxicity (cin) is a major clinical problem accounting for 12% of all hospital-acquired cases of acute kidney injury. the pathophysiology of cin is not well understood, but direct toxic effects on renal cells have been postulated as contributing to cin. we have investigated the effect of a white grape (vitis vinifera) juice extract (wgje) on human renal proximal tubular (hk-2) cells treated with the radiocontrast medium (rcm) sodium diatrizoate. wgje caused ...201525603236
characterization of the adaptive response of grapevine (cv. tempranillo) to uv-b radiation under water deficit conditions.this work aims to characterize the physiological response of grapevine (vitis vinifera l.) cv. tempranillo to uv-b radiation under water deficit conditions. grapevine fruit-bearing cuttings were exposed to three levels of supplemental biologically effective uv-b radiation (0, 5.98 and 9.66kjm(-2)day(-1)) and two water regimes (well watered and water deficit), in a factorial design, from fruit-set to maturity under glasshouse-controlled conditions. uv-b induced a transient decrease in net photosy ...201525617319
antioxidant potential of different grape cultivars against fenton-like reagent-induced liver damage ex-vivo.the phytochemicals present in the grapes are responsible for nutraceutical and health benfits due to their antioxidant properties. these phytochemicals, however, vary greatly among different cultivars. in this study, we evaluated the antioxidant potential and protective role of four different indian grape (vitis vinifera) cultivars extracts, namely flame seedless (black grapes), kishmish chorni (black with reddish brown), red globe (red) and thompson seedless mutant (sonaka, green) against the f ...201425630107
distinctive anthocyanin accumulation responses to temperature and natural uv radiation of two field-grown (vitis vinifera l.) cultivars.the responses of two red grape varieties, bovale grande (syn. carignan) and cannonau (syn. grenache), to temperature and natural uv radiation were studied in a three-years field experiment conducted in sardinia (italy), under mediterranean climate conditions. vines were covered with plastic films with different transmittances to uv radiation and compared to uncovered controls. light intensity and spectral composition at the fruit zone were monitored and berry skin temperature was recorded from v ...201525633334
cultivar specific metabolic changes in grapevines berry skins in relation to deficit irrigation and hydraulic behavior.deficit irrigation techniques are widely used in commercial vineyards. nevertheless, varieties respond differently to water availability, prompting the need to elucidate the physiological and molecular mechanisms involved in the interactions between genotypes and their environment. in the present study, the variability in berry metabolism under deficit irrigation was investigated in the field on shiraz and cabernet sauvignon (cs), known for their hydraulic variability. berry skin metabolite prof ...201525635762
promoting effect of foliage sprayed zinc sulfate on accumulation of sugar and phenolics in berries of vitis vinifera cv. merlot growing on zinc deficient soil.the effect of foliage sprayed zinc sulfate on berry development of vitis vinifera cv. merlot growing on arid zone zn-deficient soils was investigated over two consecutive seasons, 2013 and 2014. initial zinc concentration in soil and vines, photosynthesis at three berry developmental stages, berry weight, content of total soluble solids, titratable acidity, phenolics and expression of phenolics biosynthetic pathway genes throughout the stages were measured. foliage sprayed zinc sulfate showed pr ...201525648596
sugars, organic acids, and phenolic compounds of ancient grape cultivars (vitis vinifera l.) from igdir province of eastern turkey.the eurasian grapevine (vitis vinifera l.) is the most widely cultivated and economically important horticultural crop in the world. as a one of the origin area, anatolia played an important role in the diversification and spread of the cultivated form v. vinifera ssp. vinifera cultivars and also the wild form v. vinifera ssp. sylvestris ecotypes. although several biodiversity studies have been conducted with local cultivars in different regions of anatolia, no information has been reported so f ...201525654659
a fast liquid chromatography-mass spectrometry methodology for membrane lipid profiling through hydrophilic interaction liquid chromatography.in this paper, we report the development of a new method based on hilic-esi-ms for the separation of several different membrane lipid classes and their detection on a triple quadrupole mass spectrometer using precursor ion (pis) and neutral loss (nl) scanning in positive ion mode. four different columns were tested for their ability to separate, under different conditions, a mixture of 14 lipid standards containing 7 glycerophospholipids (gpl), 2 glycosphyngolipids (gsl), 3 glycolipids (gl) and ...201525655586
the phenylpropanoid pathway is controlled at different branches by a set of r2r3-myb c2 repressors in grapevine.because of the vast range of functions that phenylpropanoids possess, their synthesis requires precise spatiotemporal coordination throughout plant development and in response to the environment. the accumulation of these secondary metabolites is transcriptionally controlled by positive and negative regulators from the myb and basic helix-loop-helix protein families. we characterized four grapevine (vitis vinifera) r2r3-myb proteins from the c2 repressor motif clade, all of which harbor the ethy ...201525659381
maceration with stems contact fermentation: effect on proanthocyanidins compounds and color in primitivo red wines.three primitivo (vitis vinifera, cv.) red wines were microvinified by means of different winemaking technologies: no stem-contact fermentation destemming 100% of grapes (d100); stem-contact fermentation destemming 75%, 50% of grapes (d75-d50) respectively. the objectives of this work were to improve proanthocyanidins content in wine, to monitor the relationships between anthocyanins/tannins and to detect the effects on the polymerization state of polyphenols after 12 months storage of wines. d10 ...201525660901
shiraz wines made from grape berries (vitis vinifera) delayed in ripening by plant growth regulator treatment have elevated rotundone concentrations and "pepper" flavor and aroma.preveraison treatment of shiraz berries with either 1-naphthaleneacetic acid (naa) or ethrel delayed the onset of ripening and harvest. naa was more effective than ethrel, delaying harvest by 23 days, compared to 6 days for ethrel. sensory analysis of wines from naa-treated fruit showed significant differences in 10 attributes, including higher "pepper" flavor and aroma compared to those of the control wines. a nontargeted analysis of headspace volatiles revealed modest differences between wines ...201525661455
effects of dietary corinthian currants (vitis vinifera l., var. apyrena) on atherosclerosis and plasma phenolic compounds during prolonged hypercholesterolemia in new zealand white rabbits.corinthian currants are a rich source of phenolic compounds, which are known to exert beneficial effects on cardiovascular disease. the hypothesis tested is whether dietary supplementation with currants attenuates atherosclerosis and affects plasma phenolics during prolonged hypercholesterolemia in rabbits. thirty new zealand white rabbits were fed one of four diets (normal and supplemented with 10% currants, with 0.5% cholesterol, and with 0.5% cholesterol plus 10% currants) for eight weeks. pl ...201525662939
lipid-lowering and hepatoprotective effects of vitis vinifera dried seeds on paracetamol-induced hepatotoxicity in rats.red grape seeds as functional food are a good source of important bioactive components such as phenolics and antioxidants, which decrease oxidative stress that contributes to the pathogenesis of hepatotoxicity. the current study was conducted in order to evaluate the protective effect of red grape dried seeds (rgds) on antioxidant properties, lipid metabolism, and liver and kidney functions of rats with paracetamol (750 mg/kg) induced hepatotoxicity.201525671066
methyl jasmonate foliar application to tempranillo vineyard improved grape and wine phenolic content.the importance of phenolic compounds for grape and wine quality has drawn attention to studying different practices with the aim of increasing their content. cluster application of elicitors is a viticultural practice that has shown promising results in recent years. however, cluster application requires a previous defoliation, which is time-consuming and expensive. in the present study, methyl jasmonate was foliar applied to tempranillo grapevines in order to study its effect on grape and wine ...201525672964
jasmonates elicit different sets of stilbenes in vitis vinifera cv. negramaro cell cultures.the plant phenol trans-resveratrol, which is mainly found in grape, displays a wide range of biological effects. a cell suspension culture was developed from calli of grape leaves of vitis vinifera cv. negramaro in order to study the bioproduction of resveratrol. the effects of a number of secondary plant metabolism elicitors, namely chitosan, methyl jasmonate, jasmonic acid, coronatine, and 12-oxo-phytodienoic acid, were tested on this cell suspension culture. the identification and quantificat ...201525674504
transcriptome and metabolome reprogramming in vitis vinifera cv. trincadeira berries upon infection with botrytis cinerea.vitis vinifera berries are sensitive towards infection by the necrotrophic pathogen botrytis cinerea, leading to important economic losses worldwide. the combined analysis of the transcriptome and metabolome associated with fungal infection has not been performed previously in grapes or in another fleshy fruit. in an attempt to identify the molecular and metabolic mechanisms associated with the infection, peppercorn-sized fruits were infected in the field. green and veraison berries were collect ...201525675955
effect of maceration time on free and bound volatiles of red wines from cv. karaoğlan (vitis vinifera l.) grapes grown in arapgir, turkey.skin maceration is an essential winemaking technique that affects sensory properties and aroma of red wines. the aim of this study was to investigate aroma profiles of red wines made from cv. karaoğlan, a grape variety grown in arapgir (malatya, turkey) and the effect of maceration time (5, 10, and 15 d) on free and bound aroma compounds and sensory properties. three maceration times were applied in durations of 5, 10, and 15 d. free and glycosidically bound aroma compounds were analyzed using g ...201525677953
increased resveratrol production in wines using engineered wine strains saccharomyces cerevisiae ec1118 and relaxed antibiotic or auxotrophic selection.resveratrol is a polyphenolic compound with diverse beneficial effects on human health. red wine is the major dietary source of resveratrol but the amount that people can obtain from wines is limited. to increase the resveratrol production in wines, two expression vectors carrying 4-coumarate: coenzyme a ligase gene (4cl) from arabidopsis thaliana and resveratrol synthase gene (rs) from vitis vinifera were transformed into industrial wine strain saccharomyces cerevisiae ec1118. when cultured wit ...201525683151
berry ripening, pre-processing and thermal treatments affect the phenolic composition and antioxidant capacity of grape (vitis vinifera l.) juice.grape juice is an important dietary source of health-promoting antioxidant molecules. different factors may affect juice composition and nutraceutical properties. the effects of some of these factors (harvest time, pre-processing ethylene treatment of grapes and juice thermal pasteurization) were here evaluated, considering in particular the phenolic composition and antioxidant capacity.201625683953
proteome and transcript analysis of vitis vinifera cell cultures subjected to botrytis cinerea infection.gray mold caused by botrytis cinerea is one of the most important diseases of grapevine resulting in significant reductions in yield and fruit quality. in order to examine the molecular mechanisms that characterize the interaction between b. cinerea and the host plant, the grapevine cytoplasmic proteome was analyzed by two-dimensional polyacrylamide gel electrophoresis. the interaction between vitis vinifera cv. gamay cells and b. cinerea was characterized by the increase in spot abundance of 30 ...201525688916
optimization of extraction and microencapsulation of bioactive compounds from red grape (vitis vinifera l.) pomace.grape (vitis vinifera l.) pomace constitutes a promising source of phenolic compounds, gallic acid, flavan-3-ols, flavonoids, stilbene and anthocyanins that are beneficial for human health. the objectives of this study were to optimize the extraction and microencapsulation of red grape pomace. central composite designs with two factors were conducted for optimization using response surface methodology. the temperature (45-85 °c), and the time (2-8 h) were designed for the extraction. the results ...201525694686
characterization of polyphenols and evaluation of antioxidant capacity in grape pomace of the cv. malbec.low molecular weight polyphenols (lmw-pps) and anthocyanins, along with the antioxidant capacity, were assessed in grape pomace extract (gpe) of red grape (vitis vinifera l.) cv. malbec. twenty-six phenolics (13 lmw-pps and 13 anthocyanins) were characterized and quantified by hplc-mwd and uplc-esi-ms. the maximum concentrations of lmw-pps corresponded to the flavanols (+)-catechin and (-)-epicatechin, whereas malvidin-3-glucoside was the most abundant anthocyanin. piceatannol, a stilbene analog ...201525704698
elucidation of the inhibitory effect of phytochemicals with kir6.2 wild-type and mutant models associated in type-1 diabetes through molecular docking approach.among all serious diseases globally, diabetes (type 1 and type 2) still poses a major challenge to the world population. several target proteins have been identified, and the etiology causing diabetes has been reasonably well studied. but, there is still a gap in deciding on the choice of a drug, especially when the target is mutated. mutations in the kcnj11 gene, encoding the kir6.2 channel, are reported to be associated with congenital hyperinsulinism, having a major impact in causing type 1 d ...201425705171
soil types effect on grape and wine composition in helan mountain area of ningxia.different soil types can significantly affect the composition of wine grapes and the final wine product. in this study, the effects of soil types on the composition of cabernet sauvignon grapes and wine produced in the helan mountains were evaluated. three different representative soil types--aeolian, sierozem and irrigation silting soil were studied. the compositions of grapes and wines were measured, and in addition, the weights of 100-berry samples were determined. the grapes that grown on th ...201525706126
[comparison of stilbene synthase from different plant sources for resveratrol biosynthesis].resveratrol is a natural phytoalexin with special pharmacological and health functions. stilbene synthase (sts) is a key and rate-limiting enzyme in the biosynthesis of resveratrol that is present only in a limited number of plants. the content of resveratrol from polygonum cuspidatum is more than 1000 times higher than grapes and peanuts. we speculate that the catalytic ability of different sts may be one of the reasons causing differences in the content of resveratrol. to verify the above spec ...201425726587
yeast contribution to melatonin, melatonin isomers and tryptophan ethyl ester during alcoholic fermentation of grape musts.melatonin (mel) has been found in some medicinal and food plants, including grapevine, a commodity of particular interest for the production of wine, a beverage of economic relevance. it has also been suggested that mel in wine may, at least in part, contribute to the health-promoting properties attributed to this beverage and, possibly, to other traditional mediterranean foodstuffs. after a preliminary screening of 9 yeast strains in laboratory medium, three selected strains (saccharomyces cere ...201525726850
development of an in vivo glucosylation platform by coupling production to growth: production of phenolic glucosides by a glycosyltransferase of vitis vinifera.glycosylation of small molecules can significantly alter their properties such as solubility, stability, and/or bioactivity, making glycosides attractive and highly demanded compounds. consequently, many biotechnological glycosylation approaches have been developed, with enzymatic synthesis and whole-cell biocatalysis as the most prominent techniques. however, most processes still suffer from low yields, production rates and inefficient udp-sugar formation. to this end, a novel metabolic enginee ...201525728421
turgor and the transport of co2 and water across the cuticle (epidermis) of leaves.leaf photosynthesis relies on co₂ diffusing in while water vapour diffuses out. when stomata close, cuticle waxes on the epidermal tissues increasingly affect this diffusion. also, changes in turgor can shrink or swell a leaf, varying the cuticle size. in this study, the properties of the cuticle were investigated while turgor varied in intact leaves of hypo stomatous grape (vitis vinifera l.) or amphistomatous sunflower (helianthus annuus l.). for grape, stomata on the abaxial surface were seal ...201525737532
[determination of wine original regions using information fusion of nir and mir spectroscopy].geographical origins of wine grapes are significant factors affecting wine quality and wine prices. tasters' evaluation is a good method but has some limitations. it is important to discriminate different wine original regions quickly and accurately. the present paper proposed a method to determine wine original regions based on bayesian information fusion that fused near-infrared (nir) transmission spectra information and mid-infrared (mir) atr spectra information of wines. this method improved ...201425739204
characterization of the serine acetyltransferase gene family of vitis vinifera uncovers differences in regulation of oas synthesis in woody plants.in higher plants cysteine biosynthesis is catalyzed by o-acetylserine(thiol)lyase (oastl) and represents the last step of the assimilatory sulfate reduction pathway. it is mainly regulated by provision of o-acetylserine (oas), the nitrogen/carbon containing backbone for fixation of reduced sulfur. oas is synthesized by serine acetyltransferase (serat), which reversibly interacts with oastl in the cysteine synthase complex (csc). in this study we identify and characterize the serat gene family of ...201525741355
reduction of platelet aggregation from ingestion of oleic and linoleic acids found in vitis vinifera and arachis hypogaea oils.the purpose of this study was to evaluate the effect of the consumption of seed oils from vitis vinifera and arachis hypogaea in platelet aggregation. the initial hypothesis suggested that subjects who have consumed these seed oils undergo modified platelet aggregation. this study was performed using a pre-post test design, with a control group, and double blind. the effects of the consumption of grape seed and peanut oils were measured for platelet aggregation in clinical and laboratory tests i ...201725741817
phytotoxic metabolites from neofusicoccum parvum, a pathogen of botryosphaeria dieback of grapevine.liquid chromatography-diode array screening of the organic extract of the cultures of 13 isolates of the fungus neofusicoccum parvum, the main causal agent of botryosphaeria dieback of grapevine, showed similar metabolites. one strain was selected for further chemical studies and led to the isolation and characterisation of 13 metabolites. structures were elucidated through spectroscopic analyses, including one- and two-dimensional nmr and mass spectrometry, and through comparison to literature ...201525747381
metabolic responses to sulfur dioxide in grapevine (vitis vinifera l.): photosynthetic tissues and berries.research on sulfur metabolism in plants has historically been undertaken within the context of industrial pollution. resolution of the problem of sulfur pollution has led to sulfur deficiency in many soils. key questions remain concerning how different plant organs deal with reactive and potentially toxic sulfur metabolites. in this review, we discuss sulfur dioxide/sulfite assimilation in grape berries in relation to gene expression and quality traits, features that remain significant to the fo ...201525750643
clonal differences and impact of defoliation on sauvignon blanc (vitis vinifera l.) wines: a chemical and sensory investigation.the aim of this study, performed on sauvignon blanc clones sb11 and sb316, grafted on the same rootstock 101-14 mgt (vitis riparia × v. ruperstris) and grown at two adjacent vineyards, was two-fold: (1) to study wine chemical and sensory composition of both clones within an unaltered canopy; and (2) to determine the effect of defoliation (e.g. bunch microclimate) on wine chemical and sensory composition.201625752685
multiomics in grape berry skin revealed specific induction of the stilbene synthetic pathway by ultraviolet-c irradiation.grape (vitis vinifera) accumulates various polyphenolic compounds, which protect against environmental stresses, including ultraviolet-c (uv-c) light and pathogens. in this study, we looked at the transcriptome and metabolome in grape berry skin after uv-c irradiation, which demonstrated the effectiveness of omics approaches to clarify important traits of grape. we performed transcriptome analysis using a genome-wide microarray, which revealed 238 genes up-regulated more than 5-fold by uv-c ligh ...201525761715
transcriptome comparison of cabernet sauvignon grape berries from two regions with distinct climate.primary and secondary metabolism in grape berries is under the control of complex interactions among environmental conditions, genotypes, and management practices. to obtain an interpretation from the view of transcriptome on distinct metabolite accumulation between ecologically different regions in china, next-generation sequencing technology was performed on e-l 31, 35, and 38 stages of cabernet sauvignon grape berries from changli (cl, eastern) and gaotai (gt, western). the transcript abundan ...201525765362
de novo assembly and characterization of transcriptomes of early-stage fruit from two genotypes of annona squamosa l. with contrast in seed number.annona squamosa l., a popular fruit tree, is the most widely cultivated species of the genus annona. the lack of transcriptomic and genomic information limits the scope of genome investigations in this important shrub. it bears aggregate fruits with numerous seeds. a few rare accessions with very few seeds have been reported for annona. a massive pyrosequencing (roche, 454 gs flx+) of transcriptome from early stages of fruit development (0, 4, 8 and 12 days after pollination) was performed to pr ...201525766098
phenylalanine and urea foliar applications to grapevine: effect on wine phenolic content.phenolic compounds play a key role in the organoleptic properties of wines. viticultural practices may influence grape and wine phenolic content, thus determining their quality. the objective of this study was to evaluate the effect of foliar applications of phenylalanine and urea, at two different doses, on wine phenolic composition. grapes were harvested at their optimal technological maturity and their respective wines were elaborated at small scale. wine detailed phenolic composition was det ...201525766801
changes on grape phenolic composition induced by grapevine foliar applications of phenylalanine and urea.grapevines may require the input of nitrogen to grow and to guarantee an appropriate grape composition. recently there has been a growing interest in foliar fertilization, which entails a fast and efficient assimilation of the products. the aim of this work was to study the influence of foliar applications of phenylalanine and urea, at two different doses, on grape anthocyanins, flavonols, flavan-3-ols, phenolic acids, and stilbenes. all treatments were applied at veraison and one week later at ...201525766815
food coloring agents and plant food supplements derived from vitis vinifera: a new source of human exposure to ochratoxin a.grape pomaces are increasingly being used as starting material in the industrial production of plant food supplements (pfs), food coloring, and tartrates, but they are at risk of ochratoxin a (ota) contamination, a mycotoxin with nephrotoxic and carcinogenic effects. we analyzed 24 commercial pfs and 13 food coloring samples derived from vitis vinifera, mainly pomaces, using a hplc-fld method for ota determination. ota was found in 75% of pfs samples and 69% of food coloring samples at levels of ...201525768038
differences in volatile profiles of cabernet sauvignon grapes grown in two distinct regions of china and their responses to weather conditions.volatile compounds are considered important for plants to communicate with each other and interact with their environments. most wine-producing regions in china feature a continental monsoon climate with hot-wet summers and dry-cold winters, giving grapes markedly different growing environments compared to the mediterranean or oceanic climates described in previous reports. this study focused on comparing the volatile profiles of vitis vinifera l. cv. cabernet sauvignon berries from two regions ...201525769137
mass spectrometric and enzymatic evidence confirm the existence of anthocyanidin 3,5-o-diglucosides in cabernet sauvignon (vitis vinifera l.) grape berries.it has been widely accepted that anthocyanidin 3,5-o-diglucosides do not exist in vitis vinifera l. cabernet sauvignon (cs) berries. however, our anthocyanin analyses using hplc-esi-ms/ms detected the existence of a low level of anthocyanidin 3,5-o-diglucosides in the cabernet sauvignon grape berries grown in china. the authenticity of these samples was confirmed with microsatellite markers. the existence of anthocyanidin 3,5-o-diglucoside was further verified by the enzymatic evidence for the f ...201525771698
aflatoxins b1, b2, g1, and g2 contamination in ground red peppers commercialized in sanliurfa, turkey.aflatoxins (afs) are hepatogenic, teratogenic, imunosuppressive, and carcinogenic fungal metabolites found in feeds, nuts, wine-grapes, spices, and other grain crops. humans are exposed to afs via consumption of mycotoxin-contaminated foods. this study aimed to determine the prevalence of af contamination in powdered red peppers sold in sanliurfa. a total of 42 samples were randomly collected from retail shops, supermarkets, open bazaars, and apiaries and examined for the occurrence and levels o ...025773893
effect of irrigation regime on anthocyanin content and antioxidant activity of vitis vinifera l. cv. syrah grapes under semiarid conditions.irrigation management is a powerful technique to control grape and wine colour. however, water deficit effects on anthocyanins are often contrasting, depending on the severity of water restriction. in addition, the effect of irrigation on the antiradical capacity of grapes has not been extensively studied. in this work the effect of water availability on anthocyanin profile and content as well as the antioxidant activity of syrah grapes was investigated in an irrigation trial under semiarid clim ...201625778286
structure and functional annotation of hypothetical proteins having putative rubisco activase function from vitis vinifera.rubisco is a very large, complex and one of the most abundant proteins in the world and comprises up to 50% of all soluble protein in plants. the activity of rubisco, the enzyme that catalyzes co2 assimilation in photosynthesis, is regulated by rubisco activase (rca). in the present study, we searched for hypothetical protein of vitis vinifera which has putative rubisco activase function. the arabidopsis and tobacco rubisco activase protein sequences were used as seed sequences to search against ...201525780274
how will climate change influence grapevine cv. tempranillo photosynthesis under different soil textures?while photosynthetic responses to elevated co2, elevated temperature, or water availability have previously been reported for grapevine as responses to single stress factors, reports on the combined effect of multiple stress factors are scarce. in the present work, we evaluated effects of simulated climate change [cc; 700 ppm co2, 28/18 °c, and 33/53% relative humidity (rh), day/night] versus current conditions (375 ppm co2, 24/14 °c, and 45/65% rh), water availability (well-irrigated vs. water ...201525786733
nondestructive diagnostic test for nitrogen nutrition of grapevine (vitis vinifera l.) based on dualex leaf-clip measurements in the field.crop nitrogen status is a major issue for crop yield and quality. it is usually assessed by destructive leaf or petiole tissue analysis. a quantitative nondestructive optical estimation of n sufficiency would be a great leap forward toward precision crop management. we therefore calibrated three optical indices against leaf nitrogen content: chlorophyll (chl), epidermal flavonols, and the nitrogen balance index (nbi), which is the ratio of the former two indices. nbi was the best estimator of le ...201525801210
ampelometric leaf trait and ssr loci selection for a multivariate statistical approach in vitis vinifera l. biodiversity management.high estimated heritability values were recently revealed for mature leaf traits in grape (vitis vinifera l.), thus redeeming ampelography in the era of molecular markers. the "organisation internationale de la vigne et du vin (oiv)" set a list of hundreds of descriptors for grapevine in order to standardize ampelographic and ampelometric scores. therefore, the selection and reduction of the number of oiv codes can represent a major goal for leaner biodiversity assessment studies. the identifica ...201525801419
ethnic ancestry and smoking, farming of wine grapes in semiarid regions, and the potentials of manihot multifida in medicine use. 201525806975
physical attributes of ultisol of brazil's northeastern semiarid under organic farming of wine grapes.the purpose of this study was to evaluate the effects of organic farming of wine grapes under physical and chemical characteristics of ultisol brazil's northeastern semiarid region. the samples of soil were collected from the row and interrow of the farming and from the fallow area, at the depths of 0.0-0.10, 0.10-0.20, 0.20-0.30 and 0.30-0.60 m. the samples were collected at six and twelve months after the culture implementation to evaluate the state of aggregation, bulk density and total soil ...201525806992
association between modification of phenolic profiling and development of wine color during alcohol fermentation.to solve the problem of wine color instability in western china, different additives (the maceration enzymes vinozym g and ex-color, yeasts vr5 and red star, and commercial tannins) were added during alcoholic fermentation of syrah (vitis vinifera l.). the phenolic profile and color characteristics of wine were examined using high performance liquid chromatography mass spectrometry and cielab, respectively. the results showed that the combination of the enzyme ex-color with the red star yeast ea ...201525807971
differential regulation of dehydrin expression and trehalose levels in cardinal table grape skin by low temperature and high co2.dehydrins and trehalose are multifunctional protective biomolecules that play a role in counteracting cellular damage during dehydrative stresses. in this paper, we studied dehydrin isoform patterns, dehydrin gene expression and trehalose levels in the skin of cardinal (vitis vinifera l.) table grapes, along with their regulation by different cold postharvest storage conditions. immunoanalysis with k-segment antibody recognizes four constitutive dehydrins (from 17 to 44 kda) that are tightly reg ...201525817412
metabolic and biological profile of autochthonous vitis vinifera l. ecotypes.vitis vinifera l. is a plant species rich in phenolic compounds that are usually associated with the health benefits of wine and grape consumption in the diet. anthocyanins, catechins, flavonol, phenolic acids and stilbenes are key molecular constituents of the vitis berries, affecting the quality of grape products. the purpose of this work was to identify the metabolic profiles of 37 genetically certified v. vinifera latial accessions. in particular, qualitative and quantitative analyses of spe ...201525820686
biological, serological, and molecular characterization of a highly divergent strain of grapevine leafroll-associated virus 4 causing grapevine leafroll disease.the complete genome sequence of a highly divergent strain of grapevine leafroll-associated virus 4 (glrav-4) was determined using 454 pyrosequencing technology. this virus, designated glrav-4 ob, was detected in vitis vinifera 'otcha bala' from our grapevine virus collection at agroscope. the glrav-4 ob genome length and organization share similarities with members of subgroup ii in the genus ampelovirus (family closteroviridae). otcha bala was graft-inoculated onto indicator plants of cultivar ...201525822183
relating water deficiency to berry texture, skin cell wall composition, and expression of remodeling genes in two vitis vinifera l. varieties.the cell wall (cw) is a dynamic structure that responds to stress. water shortage (ws) impacts grapevine berry composition and its sensorial quality. in the present work, berry texture, skin cw composition, and expression of remodeling genes were investigated in two v. vinifera varieties, touriga nacional (tn) and trincadeira (tr), under two water regimes, full irrigation (fi) and no irrigation (ni). the global results allowed an evident separation between both varieties and the water treatments ...201525828510
rna-seq analysis and annotation of a draft blueberry genome assembly identifies candidate genes involved in fruit ripening, biosynthesis of bioactive compounds, and stage-specific alternative splicing.blueberries are a rich source of antioxidants and other beneficial compounds that can protect against disease. identifying genes involved in synthesis of bioactive compounds could enable the breeding of berry varieties with enhanced health benefits.201525830017
microbiological diversity and prevalence of spoilage and pathogenic bacteria in commercial fermented alcoholic beverages (beer, fruit wine, refined rice wine, and yakju).the present study examined 469 commercially available fermented alcoholic beverages (fabs), including beer (draft, microbrewed, and pasteurized), fruit wine (grape and others), refined rice wine, and yakju (raw and pasteurized). samples were screened for escherichia coli and eight foodborne pathogens (bacillus cereus, campylobacter jejuni, clostridium perfringens, escherichia coli o157:h7, listeria monocytogenes, salmonella spp., staphylococcus aureus, and yersinia enterocolitica), and the aerob ...201525836410
modelling the influence of temperature, water activity and sodium metabisulphite on the growth and ota production of aspergillus carbonarius isolated from greek wine grapes.the purpose of the present study was to develop a modelling approach to quantify the effect of temperature (15-38 °c), a(w) (0.88-0.98) and sodium metabisulphite (nambs) concentration (0-200 mg l(-1)) on the growth and ota production of aspergillus carbonarius on a grape juice based medium (gjm). growth responses of the fungus were recorded over time in terms of colony diameter changes, and fitted to the primary model of baranyi and the estimated maximum growth rates (μ(max)) and lag phases (λ) ...201525846911
timing of ripening initiation in grape berries and its relationship to seed content and pericarp auxin levels.individual berries in a grape (vitis vinifera l.) cluster enter the ripening phase at different times leading to an asynchronous cluster in terms of ripening. the factors causing this variable ripening initiation among berries are not known. because the influence via hormonal communication of the seed on fruit set and growth is well known across fruit species, differences in berry seed content and resultant quantitative or qualitative differences in the hormone signals to the pericarp likely inf ...201525848949
genetic variability and evolutionary diversification of membrane abc transporters in plants.atp-binding cassette proteins have been recognized as playing a crucial role in the regulation of growth and resistance processes in all kingdoms of life. they have been deeply studied in vertebrates because of their role in drug resistance, but much less is known about abc superfamily functions in plants.201525850033
vitis vinifera (muscat variety) seed ethanolic extract preserves activity levels of enzymes and histology of the liver in adult male rats with diabetes.the effect of v. vinifera seeds on carbohydrate metabolizing enzymes and other enzymes of the liver in diabetes is currently unknown. we therefore investigated changes in the activity levels of these enzymes following v. vinifera seed extract administration to diabetic rats. methods. v. vinifera seed ethanolic extract (250 and 500 mg/kg/day) or glibenclamide (600 μg/kg/day) was administered to streptozotocin-induced male diabetic rats for 28 consecutive days. at the end of treatment, liver was h ...201525852767
various extraction methods for obtaining stilbenes from grape cane of vitis vinifera l.grape cane, leaves and grape marc are waste products from viticulture, which can be used to obtain secondary stilbene derivatives with high antioxidant value. the presented work compares several extraction methods: maceration at laboratory temperature, extraction at elevated temperature, fluidized-bed extraction, soxhlet extraction, microwave-assisted extraction, and accelerated solvent extraction. to obtain trans-resveratrol, trans-ε-viniferin and r2-viniferin from grape cane of the v. vinifera ...201525856060
a comprehensive review of plants and their active constituents with wound healing activity in traditional iranian medicine.wound healing is a complex cascade of events with various cellular and biochemical processes that result in reconstruction and regeneration of damaged tissue. the objective of the current study was to scientifically evaluate the medicinal plants said to produce wound healing activity in traditional iranian medicine (tim).201425856320
polyphenoloxidase from riesling and dornfelder wine grapes (vitis vinifera) is a tyrosinase.polyphenoloxidases (ppo) of the type-3 copper protein family are considered to be catecholoxidases catalyzing the oxidation of o-diphenols to their corresponding quinones. ppo from grenache grapes has recently been reported to display only diphenolase activity. in contrast, we have characterized ppos from dornfelder and riesling grapes which display both monophenolase and diphenolase activity. ultracentrifugation and size exclusion chromatography indicated that both ppos occur as monomers with m ...201525863609
untargeted metabolic profiling of vitis vinifera during fungal degradation.this paper illustrates the application of an untargeted metabolic profiling analysis of winery-derived biomass degraded using four filamentous fungi (trichoderma harzianum, aspergillus niger, penicillium chrysogenum and p. citrinum) and a yeast (saccharomyces cerevisiae). analysis of the metabolome resulted in the identification of 233 significant peak features [p < 0.05; fold change (fc) > 2 and signal-to-noise ratio >50] using gas chromatography-mass spectrometry followed by statistical chemom ...201525868913
genetic diversity of stilbene metabolism in vitis sylvestris.stilbenes, as important secondary metabolites of grapevine, represent central phytoalexins and therefore constitute an important element of basal immunity. in this study, potential genetic variation in vitis vinifera ssp. sylvestris, the ancestor of cultivated grapevine, was sought with respect to their output of stilbenes and potential use for resistance breeding. considerable variation in stilbene inducibility was identified in v. vinifera ssp. sylvestris. genotypic differences in abundance an ...201525873669
new insights into the evolutionary history of plant sorbitol dehydrogenase.sorbitol dehydrogenase (sdh, ec 1.1.1.14) is the key enzyme involved in sorbitol metabolism in higher plants. sdh genes in some rosaceae species could be divided into two groups. l-idonate-5-dehydrogenase (lidh, ec 1.1.1.264) is involved in tartaric acid (ta) synthesis in vitis vinifera and is highly homologous to plant sdhs. despite efforts to understand the biological functions of plant sdh, the evolutionary history of plant sdh genes and their phylogenetic relationship with the v. vinifera li ...201525879735
vertical distribution and analysis of micro-, macroelements and heavy metals in the system soil-grapevine-wine in vineyard from north-west romania.the determination of micro-, macroelements and heavy metals in the soil-grapevine-wine system is extremely important for the wine industry, the grape and wine quality, and also for consumer health. the quantitative analysis of 10 elements: na, ca, mg, fe, cu, zn, pb, cd, ni, co were made in soil at different depths and also in grapevines (leaves and canes). for grape juice and wine there were analyzed the concentrations of cu, zn, pb, ni and cd on three cultivars fetească albă, fetească regală a ...201525883678
acclimation mechanisms elicited by sprayed abscisic acid, solar uv-b and water deficit in leaf tissues of field-grown grapevines.the independent and interactive effects of solar ultraviolet-b radiation (uv-b), moderate water deficit and sprayed abscisic acid (aba) on leaves gas exchange and biochemical aspects of field-grown grapevines of the cv. malbec were investigated in a high altitude vineyard (1450 m a.s.l.). two uv-b treatments (ambient solar uv-b or reduced uv-b), two watering treatments (well watered or moderate water deficit) and two aba treatments (no aba and sprayed aba) were given alone and combined in a fact ...201525885355
within-vineyard, within-vine, and within-bunch variability of the rotundone concentration in berries of vitis vinifera l. cv. shiraz.this study characterizes the environmental factors driving rotundone concentrations in grape berries by quantifying rotundone variability and correlating it with viticultural parameters. dissection of the vineyard into distinct zones (on the basis of vigor, electrical soil conductivity, and slope), vine into orientations to sun (shaded/unshaded), and grape bunches into sectors (upper and lower and front and back) shows the influence of vine vigor, sunlight, and temperature. occurrence of the hig ...201525891266
radicinin from cochliobolus sp. inhibits xylella fastidiosa, the causal agent of pierce's disease of grapevine.the fastidious phytopathogenic bacterium, xylella fastidiosa, poses a substantial threat to many economically important crops, causing devastating diseases including pierce's disease of grapevine. grapevines (vitis vinifera l.) planted in an area under pierce's disease pressure often display differences in disease severity and symptom expression, with apparently healthy vines growing alongside the dying ones, despite the fact that all the vines are genetic clones of one another. under the hypoth ...201525892412
host plants affect the foraging success of two parasitoids that attack light brown apple moth epiphyas postvittana (walker) (lepidoptera: tortricidae).the light brown apple moth, epiphyas postvittana is a key pest of wine grapes in australia. two parasitoids, dolichogenidea tasmanica and therophilus unimaculatus, attack the larval stage of this pest. d. tasmanica is dominant in vineyards, whereas t. unimaculatus is mainly active in native vegetation. we sought to understand why they differ in their use of habitats. plants are a major component of habitats of parasitoids, and herbivore-infested plants influence parasitoid foraging efficiency by ...201525893966
overexpression of antimicrobial lytic peptides protects grapevine from pierce's disease under greenhouse but not field conditions.pierce's disease (pd) caused by xylella fastidiosa prevents cultivation of grapevine (vitis vinifera) and susceptible hybrids in the southeastern united states and poses a major threat to the grape industry of california and texas. genetic resistance is the only proven control of x. fastidiosa. genetic engineering offers an alternative to heretofore ineffective conventional breeding in order to transfer only pd resistance traits into elite cultivars. a synthetic gene encoding lytic peptide lima- ...201525894660
estimation of the base temperature and growth phase duration in terms of thermal time for four grapevine cultivars.thermal time models have been used to predict the development of many different species, including grapevine (vitis vinifera l.). these models normally assume that there is a linear relationship between temperature and plant development. the goal of this study was to estimate the base temperature and duration in terms of thermal time for predicting veraison for four grapevine cultivars. historical phenological data for four cultivars that were collected in the pacific northwest were used to deve ...201525903759
accumulation of glycoconjugates of 3-methyl-4-hydroxyoctanoic acid in fruits, leaves, and shoots of vitis vinifera cv. monastrell following foliar applications of oak extract or oak lactone.grapevines are capable of absorbing volatile compounds present in the vineyard during the growing season, and in some cases, volatiles have been found to accumulate in fruits or leaves in glycoconjugate forms, that is, with one or more sugar moieties attached. the presence of oak lactone in wine is usually attributable to oak maturation, but oak lactone has been detected in wines made with fruit from grapevines treated with oak extract or oak lactone. this study investigated the accumulation of ...201525912091
screening of anthocyanins and anthocyanin-derived pigments in red wine grape pomace using lc-dad/ms and maldi-tof techniques.two phenolic extracts were made from a red wine grape pomace (gp) and fractionated first by sequential liquid-liquid extraction with organic solvents. the aqueous fraction was fractionated by low-pressure chromatography on toyopearl hw-40 gel and on c18. different fractions were obtained by sequential elution with aqueous/organic solvents, and then analyzed by liquid chromatography and mass spectrometry (lc-dad/ms and maldi-tof). over 50 anthocyanin-based pigments were detected by lc-dad/ms in g ...201525912410
relationship between agronomic parameters, phenolic composition of grape skin, and texture properties of vitis vinifera l. cv. tempranillo.the relationship between the agronomic parameters of grapevine and the phenolic composition of skin of vitis vinifera l. cv. tempranillo grapes was assessed. the physical and mechanical properties of berries and their skins were also determined and correlated to the chemical composition. results showed a significant negative correlation between grapevine vigor-related parameters (such as leaf area and bunch weight) and anthocyanin composition, whereas the percentage (w/w) of seeds was negatively ...201525916251
tryptophan-ethylester, the false (unveiled) melatonin isomer in red wine.among the food plants, the presence of melatonin in grapes (vitis vinifera l.) deserves particular attention because of the production of wine, an alcoholic beverage of economic relevance and with putative healthy effects. furthermore, melatonin isomers have been detected in wine too. recently, one of these isomers has been identified as tryptophan-ethylester, a compound with the same molecular weight of melatonin. in this commentary, we briefly comment the source(s) of tryptophan-ethylester in ...201525922582
computational identification of protein kinases and kinase-specific substrates in plants.the protein phosphorylation catalyzed by protein kinases (pks) plays an essential role in almost all biological progresses in plants. thus, the identification of pks and kinase-specific substrates is fundamental for understanding the regulatory mechanisms of protein phosphorylation especially in controlling plant growth and development. in this chapter, we describe the computational methods and protocols for the identification of pks and kinase-specific substrates in plants, by using vitis vinif ...201525930704
morphological and molecular characterisation of diaporthe species associated with grapevine trunk disease in china.trunk diseases in grapevine (vitis spp.) are major problems in the wine and table-grape industries reducing the productivity, quality and longevity of vineyards. species of diaporthe are important fungal pathogens of grapevine trunk disease worldwide. a survey of 14 grape vineyards located in different provinces of china was yielded diaporthe isolates associated with symptomatic grapevine wood. these isolates were identified based on morphology and a combined data matrix of rdna its, partial seq ...201525937058
quantitative proteomic analysis of cabernet sauvignon grape cells exposed to thermal stresses reveals alterations in sugar and phenylpropanoid metabolism.grapes (vitis vinifera) are a valuable fruit crop and wine production is a major industry. global warming and expanded range of cultivation will expose grapes to more temperature stresses in future. our study investigated protein level responses to abiotic stresses, with particular reference to proteomic changes induced by the impact of four different temperature stress regimes, including both hot and cold temperatures, on cultured grape cells. cabernet sauvignon cell suspension cultures grown a ...201525959233
dynamics and reversibility of the dna methylation landscape of grapevine plants (vitis vinifera) stressed by in vitro cultivation and thermotherapy.there is relatively little information concerning long-term alterations in dna methylation following exposure of plants to environmental stress. as little is known about the ratio of non-heritable changes in dna methylation and mitotically-inherited methylation changes, dynamics and reversibility of the dna methylation states were investigated in grapevine plants (vitis vinifera) stressed by in vitro cultivation. it was observed that significant part of induced epigenetic changes could be repeat ...201525973746
mirvine: a microrna expression atlas of grapevine based on small rna sequencing.mirnas are the most abundant class of small non-coding rnas, and they are involved in post-transcriptional regulations, playing a crucial role in the refinement of genetic programming during plant development. here we present a comprehensive picture of mirna regulation in vitis vinifera l. plant during its complete life cycle. furthering our knowledge about the post-transcriptional regulation of plant development is fundamental to understand the biology of such an important crop.201525981679
antioxidant effect of a polyphenol-rich grape pomace extract on motility, viability and lipid peroxidation of thawed bovine spermatozoa.grape extracts of the greek species vitis vinifera possess potent antioxidant properties in vitro. the freeze/thaw process and the preparation of semen during assisted reproductive techniques can adversely affect the functional integrity of spermatozoa. the objective was to assess the effect of three different concentrations (1 μg ml(-1), 2 μg ml(-1) and 5 μg ml(-1)) of a polyphenol-rich grape pomace extract on motility, viability, acrosomal and lipid peroxidation status of thawed bovine spermat ...201425984501
vitis vinifera vvwrky13 is an ethylene biosynthesis-related transcription factor.a novel transcription factor vvwrky13 was cloned from vitis vinifera and found functioning in ethylene biosynthesis pathway by mediating acs2 and acs8 expression. grapevine is one of the most economically important plants, and ethylene is a plant hormone related with its growth, development, abiotic and biotic resistance. until now, the regulators and their mechanism of ethylene biosynthesis are still not well understood. we have cloned a novel gene from a grapevine cultivar 'zuoyouhong' and nam ...201526001999
study of polyphenol content in the seeds of red grape (vitis vinifera l.) varieties cultivated in turkey and their antiradical activity.the polyphenolic content and antioxidant capacity in the seeds of 11 red grape varieties (five international and six native) widely cultivated in turkey were investigated. total phenolic, total flavanol and total polymeric procyanidin content ranged from 79.2 to 154.6, 89.2 to 179.4, and 27.0 to 43.3mg/g seed, respectively. while (+)-catechin (4.71-23.8mg/g seed) was found as main flavanol, galloylated catechin monomer and dimeric procyanidin amounts varied between 2.89-17.2 and 0.97-2.97mg/g se ...200826003368
screening of anthocyanins in single red grapes using a non-destructive method based on the near infrared hyperspectral technology and chemometrics.anthocyanins are the main compounds responsible for the colour of red wines and therefore it may be important to evaluate the content of the aforesaid secondary metabolites during grape ripening due to the crucial importance to determine wine colour. nowadays, there is a growing demand of rapid and non-destructive analytical tools for analysing grapes, such as the emerging hyperspectral analysis.201626009839
differentiation of grapevine (vitis vinifera l.) conculta members based on molecular tools.twenty-seven grapevine (vitis vinifera l.) varieties within 12 putative berry colour variation groups (conculta) were genotyped with 14 highly polymorphic microsatellite (simple sequence repeats (ssr)) markers. three additional oligonucleotide primers were applied for the detection of the gret1 retroelement insertion in the promoter region of vvmyba1 transcription factor gene regulating the ufgt (udp-glucose: flavonoid 3-o-glucosyltransferase) activity. ufgt is the key enzyme of the anthocyanin ...201426019484
ssr and srap marker-based linkage map of vitis vinifera l.an f1 population was created by the cross '87-1' × '9-22'. the female parent '87-1' was an extremely early maturing cultivar with strong flavour. the male parent was an excellent breeding line producing large berries maturing late. the mapping population included 149 randomly chosen individuals. molecular genetic map for each parent and the consensus map were constructed using simple sequence repeat and sequence-related amplified polymorphism markers by software joinmap 3.0. the '87-1' map cover ...201426019507
molecular cloning, sequence characterization and expression pattern of rab18 gene from watermelon (citrullus lanatus).the complete mrna sequence of watermelon rab18 gene was amplified through the rapid amplification of cdna ends (race) method. the full-length mrna was 1010 bp containing a 645 bp open reading frame, which encodes a protein of 214 amino acids. sequence analysis revealed that watermelon rab18 protein shares high homology with the rab18 of cucumber (99%), muskmelon (98%), morus notabilis (90%), tomato (89%), wine grape (89%) and potato (88%). phylogenetic analysis revealed that watermelon rab18 gen ...201526019638
impedance of the grape berry cuticle as a novel phenotypic trait to estimate resistance to botrytis cinerea.warm and moist weather conditions during berry ripening provoke botrytis cinerea (b. cinerea) causing notable bunch rot on susceptible grapevines with the effect of reduced yield and wine quality. resistance donors of genetic loci to increase b. cinerea resistance are widely unknown. promising traits of resistance are represented by physical features like the thickness and permeability of the grape berry cuticle. sensor-based phenotyping methods or genetic markers are rare for such traits. in th ...201526024417
climate change conditions (elevated co2 and temperature) and uv-b radiation affect grapevine (vitis vinifera cv. tempranillo) leaf carbon assimilation, altering fruit ripening rates.the increase in grape berry ripening rates associated to climate change is a growing concern for wine makers as it rises the alcohol content of the wine. the present work studied the combined effects of elevated co2, temperature and uv-b radiation on leaf physiology and berry ripening rates. three doses of uv-b: 0, 5.98, 9.66 kjm(-2)d(-1), and two co2-temperature regimes: ambient co2-24/14 °c (day/night) (current situation) and 700 ppm co2-28/18 °c (climate change) were imposed to grapevine frui ...201526025530
ethnomedicinal plants of sarigöl district (manisa), turkey.this paper provides new and significant ethnopharmacological information on medicinal plants used by native people who live in sarigöl and its surroundings in turkey.201526026370
preharvest salicylic acid treatments to improve quality and postharvest life of table grapes (vitis vinifera l.) cv. flame seedless.significance of preharvest salicylic acid (sa) treatments on maturity, quality and postharvest life of grape cv. flame seedless were studied during two years. the experiment was performed on 12-year old own rooted, grapevines planted at 3 m × 3 m spacing trained on overhead system. vines were treated with aqueous solutions of sa (0.0, 1.0, 1.5 and 2.0 mm) at pea stage and at veraison. after harvesting, clusters were divided into two lots in which one was subjected to initial quality evaluation, ...201526028743
copper tolerance and biosorption of saccharomyces cerevisiae during alcoholic fermentation.at high levels, copper in grape mash can inhibit yeast activity and cause stuck fermentations. wine yeast has limited tolerance of copper and can reduce copper levels in wine during fermentation. this study aimed to understand copper tolerance of wine yeast and establish the mechanism by which yeast decreases copper in the must during fermentation. three strains of saccharomyces cerevisiae (lab selected strain bh8 and industrial strains awri r2 and freddo) and a simple model fermentation system ...201526030864
chemical composition of volatile aroma metabolites and their glycosylated precursors that can uniquely differentiate individual grape cultivars.every grape cultivar has its own unique genetic characteristics, leading to the production of a different secondary metabolite profile. aroma is one of the most important aspects in terms of the quality of grapes and previous studies have assigned specific aromas to particular grape cultivars. in this study we present the molecular profiling of volatile aroma metabolites and their precursors in ten selected genotypes, including six vitis vinifera cultivars, two american species (arizonica texas, ...201526041197
ozone fumigation for safety and quality of wine grapes in postharvest dehydration.this paper proposes postharvest ozone fumigation (as a method) to control microorganisms and evaluate the effect on polyphenols, anthocyanins, carotenoids and cell wall enzymes during the grape dehydration for wine production. pignola grapes were ozone-treated (1.5 g/h) for 18 h (a=shock treatment), then dehydrated or ozone-treated (1.5 g/h) for 18 h and at 0.5 g/h for 4 h each day (b=long-term treatment) during dehydration. treatment and dehydration were performed at 10 °c. no significant diffe ...201526041242
sesquiterpene volatile organic compounds (vocs) are markers of elicitation by sulfated laminarine in grapevine.inducing resistance in plants by the application of elicitors of defense reactions is an attractive plant protection strategy, particularly for grapevine (vitis vinifera), which is susceptible to severe fungal diseases. although induced resistance (ir) can be successful under controlled conditions, in most cases, ir is not sufficiently effective for practical disease control under outdoor conditions. progress in the application of ir requires a better understanding of grapevine defense mechanism ...201526042139
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