Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| diversity of ixodes-borne borrelia species--clinical, pathogenetic, and diagnostic implications and impact on vaccine development. | among borrelia spirochetes carried by hard ticks belonging to the various ixodes species, at least 10 species can be distinguished. of these, borrelia burgdorferi sensu stricto is involved in human lyme borreliosis in north america and europe, and borrelia garinii and borrelia afzelii in human disease in europe and asia. the pathogenetic significance of the other species is uncertain. although some of the borrelia species are restricted to certain tick species, ixodes ricinus, the vector of lyme ... | 2002 | 12804166 |
| improved serodiagnosis of erythema migrans using novel recombinant borrelial bbk32 antigens. | the performances of recombinant borrelial bbk32 proteins as antigens in the serology of erythema migrans (em) were evaluated in an elisa. serum samples were obtained from 75 patients from different geographic areas where three borrelial species, borrelia burgdorferi sensu stricto, borrelia afzelii or borrelia garinii, cause lyme borreliosis. antibodies to variant bbk32 proteins were compared with anti-flagella or with anti-ir(6) peptide antibodies. in igg elisa at presentation of em, 65/75 (87 % ... | 2003 | 12808077 |
| [comparative analysis of use of two strains of various genotypes of borrelia burgdorferi sensu lato as antigens for antibody identification in ixodes tick borreliosis by indirect immunofluorescence]. | the results of the indirect reaction of immune-fluorescence (irif) was studied in testing 49 sera of 19 patients with lyme-borreliosis with antigens of genotypes borrelia afzeli (strain jp-21) and borrelia burgdorferi sensu stricto (strain no. 17); rabbit fluorescini-sothiocyanate-marked conjugates to human immunoglobulins m and g as well as polyvalent conjugate were used of. no reliable differences were found between all positive and all negative results. the biggest portion of positive results ... | 2003 | 12822309 |
| genetic diversity of bacterial agents detected in ticks removed from asymptomatic patients in northeastern italy. | a total of 360 ticks were removed from 353 asymptomatic subjects in belluno province, italy and surrounding areas, from 1998 to 2001. ticks were identified as ixodes ricinus (357), ixodes hexagonus (1), rhipicephalus sanguineus (1), and ixodes ventalloi (1). tick dna was investigated by pcr and subsequent sequencing of amplified products to identity associated bacterial agents. primers targeting different genes of rickettsia (glta and ompa), borrelia (16s rdna, rpob), francisella (16s rdna), and ... | 2003 | 12860623 |
| co-feeding transmission and its contribution to the perpetuation of the lyme disease spirochete borrelia afzelii. | 2003 | 12899145 | |
| lyme borreliosis. | lyme borreliosis is a multi-organ infection caused by spirochetes of the borrelia burgdorferi sensu lato group with its species b burgdorferi sensu stricto, borrelia garinii, and borrelia afzelii, which are transmitted by ticks of the species ixodes. laboratory testing of lyme borreliosis includes culture, antibody detection using elisa with whole extracts or recombinant chimeric borrelia proteins, immunoblot, and pcr with different levels of sensitivity and specificity for each test. common ski ... | 2003 | 12901891 |
| first isolation and characterization of borrelia burgdorferi sensu lato strains from ixodes ricinus ticks in turkey. | in order to investigate the presence and prevalence of lyme borreliosis (lyme disease) borrelia species, 312 unfed ticks were collected by flagging at a woodland area in trakya, in the european side of turkey, in may 2002. twelve of 299 ixodes ricinus ticks were infected with borrelia spp., as determined by cultivation in bsk medium (prevalence rate 4.0 %). ten pure cultures were subjected to further characterization by sequencing analysis of the 5s-23s rdna intergenic spacer, 16s rdna and flage ... | 2003 | 12909659 |
| identification of borrelia burgdorferi sensu lato, anaplasma and ehrlichia species, and spotted fever group rickettsiae in ticks from southeastern europe. | prevalence data for tick-borne pathogens are used to assess the risk for human health. in this study the presence and identity of borrelia burgdorferi sensu lato, ehrlichia, anaplasma, and rickettsia species in bulgarian ixodes ricinus ticks and in non- ixodes ticks from turkey and albania was determined by polymerase chain reaction (pcr) and reverse line blot hybridization. in the adult bulgarian ticks, the prevalence of borrelia burgdorferi sensu lato infection was approximately 40%, while bor ... | 2003 | 12938010 |
| dynamics of cytokine production in human peripheral blood mononuclear cells stimulated by lps or infected by borrelia. | to specify the role of individual cytokines in the immune response to pyrogens, isolated and cultivated human peripheral blood mononuclear cells (pbmc) were used for the experiments. different pyrogens (lipopolysaccharide from escherichia coli - lps and live borrelia afzelii) were applied and the time course of changes in concentrations of different cytokines in the medium was followed using the elisa method. it was found that nonstimulated human pbmc proliferate under in vitro conditions and pr ... | 2003 | 14535835 |
| tick-host-borrelia population interactions: long-term records in eastern europe. | during 10 spring and summer seasons (1992-2001), the dynamics of the epizootic process in natural foci were studied in taiga forests of the pre-ural region, russia. in these foci, borrelia garinii, borrelia afzelii and tick-borne encephalitis (tbe) virus circulate transmitted by their main vector tick, ixodes persulcatus. main parameters of the epizootic process were calculated for each season. in three seasons (1993, 1996, and 1999) all parameters characterizing the abundance of unfed nymphs an ... | 2002 | 14570135 |
| detection and identification of pathogens and host dna in unfed host-seeking ixodes ricinus l. (acari: ixodidae). | in this study, we have developed molecular methods for the identification of reservoir hosts of sylvatic tick-borne zoonoses. the methods are based on the analysis of the blood meal remnant in the tick gut and include detection of pathogens and identification of the host origin of the blood meal. for host identification, a universal primer pair was used to amplify part of the vertebrate 18s rrna gene followed by reverse line blot hybridization using subgroup-specific probes. analyses of dna from ... | 2003 | 14596289 |
| genetic diversity among borrelia strains determined by single-strand conformation polymorphism analysis of the ospc gene and its association with invasiveness. | lyme borreliosis (lb) is a tick-borne spirochetal infection caused by three borrelia species: borrelia afzelii, b. garinii, and b. burgdorferi sensu stricto. lb evolves in two stages: a skin lesion called erythema migrans and later, different disseminated forms (articular, neurological, cardiac.). previous research based on analysis of ospc sequences allowed the definition of 58 groups (divergence of <2% within a group and >8% between groups). only 10 of these groups include all of the strains i ... | 2003 | 14605139 |
| improved serodiagnosis of early lyme borreliosis: immunoblot with local borrelia afzelii strain. | to improve the serodiagnosis of lyme borreliosis (lb) the performances of four tests were evaluated. an indirect immunofluorescent assay based on borrelia burgdorferi s.s., enzyme-linked immunosorbent assay (elisa) based on local isolates of borrelia afzelii and b. burgdorferi s.s., and immunoblot (ib) of b. afzelii were prepared. the serum panels contained 214 serum samples: control group (n=120) and patients at different stages of lb (n=94). the specificity of ib was 96%, of in-house elisa 93% ... | 2003 | 14629271 |
| evidence of babesia microti infection in multi-infected ixodes persulcatus ticks in russia. | to detect babesia-infected ixodes persulcatus shulze in a suburb of st. petersburg, russia, 738 adult ticks were studied using babesia specific primers and pcr techniques. the entire sample (more than 1,200 individuals) was screened for the presence of borrelia spp., ehrlichia spp. and tick-borne encephalitis virus (tbev). all 7 ticks infected with babesia microti, were also infected with other pathogens (all 7 among 417 infected ticks, zero amongst the remaining 321 naive ones (chi2 = 5.25, p<0 ... | 2003 | 14635819 |
| solitary and multiple erythema migrans in children: comparison of demographic, clinical and laboratory findings. | data on european children with erythema migrans (em) are limited. | 2003 | 14735383 |
| dynamics of cytokine production in human peripheral blood mononuclear cells stimulated by lps or infected by borrelia. | to specify the role of individual cytokines in the immune response to pyrogens, isolated and cultivated human peripheral blood mononuclear cells (pbmc) were used for the experiments. different pyrogens (lipopolysaccharide from escherichia coli - lps and live borrelia afzelii) were applied and the time course of changes in concentrations of different cytokines in the medium was followed using the elisa method. it was found that nonstimulated human pbmc proliferate under in vitro conditions and pr ... | 2003 | 14964289 |
| evaluation of groel gene analysis for identification of borrelia burgdorferi sensu lato. | the nucleotide sequences of the groel genes, the flagellin genes, and the 16s rrna genes from 22 reference strains of borrelia were compared. groel sequence analysis is useful not only in interspecies differentiation but also in intraspecies differentiation of borrelia afzelii and borrelia garinii isolates. | 2004 | 15004091 |
| comparison of erythema migrans caused by borrelia afzelii and borrelia garinii. | we compared epidemiological and clinical characteristics of patients with erythema migrans (em) caused by borrelia afzelii and borrelia garinii. | 2004 | 15007737 |
| occurrence of borrelia afzelii and borrelia garinii in ixodes ricinus ticks from southern moravia, czech republic. | during the years 1996-2000, a total of 2398 ixodes ricinus ticks were collected in three areas of southern moravia and eastern bohemia, czech republic, and examined by dark-field microscopy for the presence of spirochetes. the prevalence of b. burgdorferi sensu lato in ixodes ricinus ticks varied and depended upon the year. in 1996, the prevalence 6.8% was observed, during 1997 and 1998, it increased to 8.4% and 12.3%, respectively. the lowest prevalence was observed in 1999 (3.6%), and in 2000 ... | 2004 | 15018772 |
| lyme borreliosis agents and the genetics and sex of their vector, ixodes ricinus. | the tick ixodes ricinus is responsible for the transmission and maintenance of a wide variety of pathogenic organisms in the northern hemisphere, among which lyme disease represents a major threat to humans. despite numerous studies, the epidemiology of the different bacterial species responsible for this disease remains unclear. recent evidence for a sex-biased genetic structure of its european vector leads us to analyse the consequences of this pattern on borrelia transmission. here we show th ... | 2004 | 15026017 |
| adaptation of diverse lyme disease spirochetes in a natural rodent reservoir host. | we compared the relative reservoir competence of european wood mice for two genospecies of lyme disease spirochetes by analyzing susceptibility, intrinsic incubation period, and degree and duration of infectivity. borrelia afzelii, specializing in particular reservoir hosts, is better adapted to those hosts than is the more generalist genospecies b. burgdorferi sensu stricto. | 2004 | 15039378 |
| improvement in the laboratory recognition of lyme borreliosis with the combination of culture and pcr methods. | lyme disease is a multisystem, multistage infection caused by three genospecies of the borrelia burgdorferi sensu lato species. the diagnosis of lyme disease is based on a history of tick-bite, physical examination, and serological tests. in the seronegative patients with lyme borreliosis symptoms, additional testing should be introduced. | 2003 | 15068385 |
| identification of three clinically relevant borrelia burgdorferi sensu lato genospecies by pcr-restriction fragment length polymorphism analysis of 16s-23s ribosomal dna spacer amplicons. | we report the results of a study of the prevalences of three clinically relevant borrelia burgdorferi sensu lato genospecies (borrelia burgdorferi sensu stricto, borrelia afzelii, and borrelia garinii) in 1,040 questing ixodes ticks from all regions of latvia, where lyme borreliosis is endemic. the prevalences of borrelia in ixodes ricinus and ixodes persulcatus were 22.6 and 27.9%, respectively. molecular typing of b. burgdorferi from infected ticks was performed by restriction fragment length ... | 2004 | 15070987 |
| lysine-dependent multipoint binding of the borrelia burgdorferi virulence factor outer surface protein e to the c terminus of factor h. | serum resistance, an important virulence determinant of borrelia burgdorferi sensu lato strains belonging to the borrelia afzelii and b. burgdorferi sensu stricto genotypes, is related to binding of the complement inhibitor factor h to the spirochete surface protein outer surface protein e (ospe) and its homologues. in this study, we show that the c-terminal short consensus repeats 18-20 of both human and mouse factor h bind to ospe. analogously, factor h-related protein 1, a distinct plasma pro ... | 2004 | 15128807 |
| serological description of estonian patients with lyme disease, a comparison with control sera from endemic and non-endemic areas. | serological tests for lyme disease are mostly not well standardized and cases of misinterpretation of test results by clinicians are rather common. the diagnostic value of serologic tests may also depend on the seroepidemiological situation of the population. the aim of the study was to compare the immunoblot pattern of lyme borreliosis patients and control sera from endemic and non-endemic regions and to identify the most suitable interpretation criteria for our immunoblot test. serum samples o ... | 2004 | 15147004 |
| sequence typing reveals extensive strain diversity of the lyme borreliosis agents borrelia burgdorferi in north america and borrelia afzelii in europe. | the genetic polymorphism of borrelia burgdorferi and borrelia afzelii, two species that cause lyme borreliosis, was estimated by sequence typing of four loci: the rrs-rrla intergenic spacer (igs) and the outer-membrane-protein gene p66 on the chromosome, and the outer-membrane-protein genes ospa and ospc on plasmids. the major sources of dna for pcr amplification and sequencing were samples of the b. burgdorferi tick vector ixodes scapularis, collected at a field site in an endemic region of the ... | 2004 | 15184561 |
| [eosinophilic fasciitis associated with borrelia afzelii infection]. | a 58-year-old woman suffered from stiffness, swelling, rubor and muscle pain in the extremities one month after she climbed a mountain in kyushu, an island in southern japan. on admission, mild proximal weakness was present in the extremities. her range of motion in the extremities was limited due to firm skin and subcutaneous stiffness which was similar to scleroderma. she showed peripheral blood eosinophilia and hypergammaglobulinemia as well as a high erythrocyte sedimentation rate. an igm an ... | 2004 | 15287514 |
| diversity within borrelia burgdorferi sensu lato genospecies in switzerland by reca gene sequence. | a total of 874 ixodes ricinus ticks were collected in switzerland to investigate the genetic diversity of the borrelia population. we integrated to the rt-pcr method the dna sequence analysis of a 162-bp fragment of the reca gene. five genospecies were detected: borrelia afzelii, borrelia burgdorferi s.s., borrelia garinii, borrelia valaisiana, and borrelia lusitaniae. a heterogeneous distribution was observed within the b. burgdorferi s.l. genospecies. the most prevalent and diverse genospecies ... | 2004 | 15336411 |
| molecular characterization of the ospa(161-175) t cell epitope associated with treatment-resistant lyme arthritis: differences among the three pathogenic species of borrelia burgdorferi sensu lato. | treatment-resistant lyme arthritis, which may result from infection-induced autoimmunity, is associated with reactivity to a t cell epitope of outer-surface protein a (ospa(161-175)) of borrelia burgdorferi sensu stricto (bb). this syndrome has been noted primarily in the united states where only bb is present, and rarely in europe where borrelia garinii (bg) and borrelia afzelii (ba) predominate. to gain a better understanding of this epitope, we identified its species-specific polymorphisms, d ... | 2004 | 15501399 |
| prevalence of borrelia burgdorferi sensu lato genospecies in host-seeking ixodes ricinus ticks in selected south bohemian locations (czech republic). | in selected localities of ceské budĕjovice and ceský, krumlov districts, well known by stable high incidence of tick-borne encephalitis (tbe) human cases but with low incidence of lyme borreliosis, monitoring of borrelia burgdorferi sensu lato (s.l.) in ixodes ricinus ticks was performed. research was also aimed at the spread of i. ricinus to mountain areas of this region (national park sumava), as well as at investigating this tick for b. burgdorferi s.l. genospecies and tbe virus infection. al ... | 2004 | 15508414 |
| relationships of a novel lyme disease spirochete, borrelia spielmani sp. nov., with its hosts in central europe. | to determine whether the pathogenic variant of lyme disease spirochetes, isolate a14s, is perpetuated in a particular reservoir-vector relationship, we screened vector ticks in various central european sites for a related spirochete and determined its host association. a14s-like spirochetes infect numerous questing ticks in the petite camargue alsacienne (pc). they frequently infect dormice, but no mice or voles. garden dormice appear to be better reservoir hosts for a14s-like spirochetes than f ... | 2004 | 15528500 |
| comparison of growth of borrelia afzelii, b. garinii, and b. burgdorferi sensu stricto in mkp and bsk-ii medium. | lyme borreliosis is a tick-borne disease caused by genetically diverse borrelia strains including b. afzelii, b. garinii, and b. burgdorferi sensu stricto (s.s.). the aim of the present study was to assess and compare the growth of one strain per species of b. afzelii, b. garinii, and b. burgdorferi s.s. in modified kelly-pettenkofer (mkp) and barbour-stonner-kelly-ii (bsk-ii) medium, and to check for the presence of the overgrowth after inoculating the media with a mixture of two different borr ... | 2004 | 15595391 |
| terrestrial distribution of the lyme borreliosis agent borrelia burgdorferi sensu lato in east asia. | lyme borreliosis is the most prevalent tick-borne zoonosis and an important emerging infection in europe, north america, and far eastern countries. the geographical distribution of borrelia spp. and the relationship between borrelia spp. and tick spp. in east asian countries have been studied. in northern asian countries, ixodes persulcatus carries eurasian-type borrelia garinii (20047 type), borrelia afzelii, and asian-type b. garinii (variant nt29), whereas borrelia burgdorferi sensu stricto h ... | 2004 | 15623946 |
| characterization of borrelia burgdorferi sensu lato isolated in moscow province--a sympatric region for ixodes ricinus and ixodes persulcatus. | the relationships between borrelia species and the vector ticks, ixodes persulcatus and ixodes ricinus were examined in a molecular epidemiological study. we conducted a survey in the moscow region which is a sympatric region for both species of tick. we examined 630 unfed i. ricinus and i. persulcatus, ticks collected from four different regions around moscow within an area of 250 km2. eighty-four ticks were culture positive (13.3%) and the prevalence rate varied in each region from 5.7% to 42. ... | 2005 | 15715174 |
| effect of tick salivary gland extract on the cytokine production by mouse epidermal cells. | previous studies have demonstrated that both tick saliva and borrelia burgdorferi sensu lato antigens modulate the cytokine response of the host. in this paper, the effect of salivary gland extract (sge) from ixodes ricinus (l., 1758) ticks on cytokine production by primary cultures of mouse epidermal cells stimulated with borrelia afzelii canica, nato, du merle, mazie, baranton et postic, 1993 spirochetes was analysed. epidermal cells were derived from c3h/hen mice, susceptible to lyme disease, ... | 2004 | 15729950 |
| identification of a new borrelia species among small mammals in areas of northern spain where lyme disease is endemic. | the role of small mammals as reservoir hosts for borrelia burgdorferi was investigated in several areas where lyme disease is endemic in northern spain. a low rate of infestation by ixodes ricinus nymphs was found in the small mammal populations studied that correlated with the near-absence of b. burgdorferi sensu lato in 184 animals tested and with the lack of transmission of b. burgdorferi sensu lato to i. ricinus larvae that fed on them. in contrast, questing ticks collected at the same time ... | 2005 | 15746336 |
| improving the specificity of 16s rdna-based polymerase chain reaction for detecting borrelia burgdorferi sensu lato-causative agents of human lyme disease. | 16s rdna sequences of borrelia burgdorferi sensu lato were aligned with the 16s rdna sequences of borrelia hermsii, borrelia turicatae, and borrelia lonestari in order to identify primers that might be used to more specifically identify agents of human lyme disease in ticks in human skin samples. | 2005 | 15752343 |
| diversity of borrelia burgdorferi sensu lato in russian far east. | thirty strains of borrelia burgdorferi sensu lato have been isolated from ixodes persulcatus ticks and from skin lesions of lyme disease patients in the russian far east from 1997 to 2003. we amplified full-length outer surface protein a (ospa) gene of all strains. blast search and following phylogenetic analysis showed that strains form four well-defined groups. four strains belong to borrelia afzelii species. other strains distributed into tree major groups, identified as borrelia garinii. ind ... | 2005 | 15781992 |
| identification and functional characterization of complement regulator-acquiring surface protein 1 of the lyme disease spirochetes borrelia afzelii and borrelia garinii. | complement regulator-acquiring surface protein 1 (crasp-1) is the dominant factor-h-like protein 1 (fhl-1)- and factor-h-binding protein of borrelia burgdorferi and is suggested to contribute to persistence of the pathogen. the prototype crasp-1 of b. burgdorferi sensu stricto (crasp-1bb) has been formerly characterized. as shown recently, serum-resistant borrelia afzelii strains express a unique fhl-1 and factor h-binding protein, designated crasp-1ba. here, we describe for the first time the i ... | 2005 | 15784581 |
| in vitro susceptibility testing of borrelia burgdorferi sensu lato isolates cultured from patients with erythema migrans before and after antimicrobial chemotherapy. | clinical treatment failures have been reported to occur in early lyme borreliosis (lb) for many suitable antimicrobial agents. investigations of possible resistance mechanisms of the borrelia burgdorferi complex must analyze clinical isolates obtained from lb patients, despite their receiving antibiotic treatment. here, borrelial isolates obtained from five patients with erythema migrans (em) before the start of antibiotic therapy and again after the conclusion of treatment were investigated. th ... | 2005 | 15793100 |
| susceptibility of borrelia afzelii strains to antimicrobial agents. | the aim of the present study was to determine the susceptibility of borrelia afzelii strains to antibiotics, and to test the hypothesis that persistence of borrelia in skin, after therapy, is a consequence of resistance to the antibiotic used for treatment. ten b. afzelii strains isolated from skin of seven adult patients (two with acrodermatitis chronica atrophicans, five with erythema migrans) were studied. in three patients b. afzelii was isolated from erythema migrans lesion before antibioti ... | 2005 | 15871918 |
| risk of tick-borne bacterial diseases among workers of roztocze national park (south-eastern poland). | the objective of the present study was to assess the risk of borreliosis and anaplasmosis (ehrlichiosis) among the forestry workers of the roztocze national park (south-eastern poland) by examination of ixodes ricinus ticks living in park area with pcr method, and by the serological and clinical examination of the workers. in 406 examined ticks, the prevalence of infection with borrelia burgdorferi sensu lato was 11.5 %. the nested pcr reaction for determining the genospecies showed that the mos ... | 2005 | 16028877 |
| solving a sticky problem: new genetic approaches to host cell adhesion by the lyme disease spirochete. | the lyme disease spirochetes, comprised of at least three closely related species, borrelia burgdorferi, borrelia garinii and borrelia afzelii, are fascinating and enigmatic bacterial pathogens. they are maintained by tick-mediated transmission between mammalian hosts, usually small rodents. the ability of these bacteria, which have relatively small genomes, to survive and disseminate in both an immunocompetent mammal and in an arthropod vector suggests that they have evolved elegant and indispe ... | 2005 | 16101994 |
| [genetic variants of borrelia afzelii, a pathogen of the ixodes tick borrelioses]. | borrelia afzelii is one of two most important pathogens of the ixodes tick borrelioses (itb) in russia and neighboring countries. this pathogen circulates in various ecosystems and has a wide range of reservoir hosts and transmitters. the results of studies of genetic heterogeneity of the spirochaetae b. afzelii are considered. a total of 139 primary isolates were studied. the isolates were isolated from three species of ixodes ticks at different stages of development and obtained from the labor ... | 2005 | 16173394 |
| tickborne pathogen detection, western siberia, russia. | ixodes persulcatus (n = 125) and dermacentor reticulatus (n = 84) ticks from western siberia, russia, were tested for infection with borrelia, anaplasma/ehrlichia, bartonella, and babesia spp. by using nested polymerase chain reaction assays with subsequent sequencing. i. persulcatus ticks were infected with borrelia burgdorferi sensu lato (37.6% +/- 4.3% [standard deviation]), anaplasma phagocytophilum (2.4% +/- 1.4%), ehrlichia muris (8.8% +/- 2.5%), and bartonella spp. (37.6% +/- 4.3%). d. re ... | 2005 | 16318722 |
| hosts and pathogen detection for immature stages of ixodes ricinus (acari: ixodidae) in north-central spain. | to determine hosts of the immature stages of a southern population of ixodes ricinus, we trapped rodents and birds in an area of north-central spain in may-june and august-september of 1998 and 1999. the most frequently trapped rodents were apodemus sylvaticus (230 specimens) and clethrionomys glareolus (99), with a larval infestation prevalence of 49% and 81%, respectively (in spring) and 21% and 41% (in summer). c. glareolus was always more heavily parasitized by larvae (mean numbers 19.8 in s ... | 2005 | 16323055 |
| [lyme borreliosis--an overview]. | north american lyme disease and european borreliosis differ in many aspects: whereas in north america infection by borrelia burgdorferi sensu stricto almost invariably leads to erythema migrans, frequently followed by oligoarthritis in untreated individuals, most infections in europe are caused by borrelia garinii and borrelia afzelii and are contracted asymptomatically. a minority of the infected individuals develop neuroborreliosis, consisting of various combinations of meningitis, radiculitis ... | 2005 | 16350535 |
| lyme borreliosis: insights into tick-/host-borrelia relations. | lyme borreliosis (lb) is a serious infectious disease of humans and some domestic animals in temperate regions of the northern hemisphere. it is caused by certain spirochetes in the borrelia burgdorferi sensu lato (s.l.) species complex. the complex consists of 11 species (genospecies). borrelia burgdorferi sensu stricto (s.s.), borrelia garinii and borrelia afzelii are the major agents of human disease. borrelia burgdorferi s.l. species are transmitted mainly by ticks belonging to the ixodes ri ... | 2005 | 16405291 |
| serological confirmation of borrelia burgdorferi infection in dogs in the czech republic. | from the epidemiological point of view, dogs are very important since they are considered a suitable indicator of the spread of human borreliosis. serum samples obtained from healthy, asymptomatic military dogs from 12 different areas in the czech republic were examined for igg antibodies to borrelia burgdorferi sensu lato (s.l.). the total of 399 serum samples were tested by a whole-cell elisa. specific antibodies to borrelia burgdorferi s.l. were detected in 26 cases (6.5%). in different local ... | 2006 | 16437298 |
| co-inoculation of borrelia afzelii with tick salivary gland extract influences distribution of immunocompetent cells in the skin and lymph nodes of mice. | the impact of ixodes ricinus salivary gland extract (sge) on inflammatory changes in the skin and draining lymph nodes of mice, elicited by the infection with the important human pathogen, b. afzelii, was determined using flow cytometry. sge injected together with spirochetes reduced the numbers of leukocytes and gammadelta-t lymphocytes in infected epidermis at early time-points post infection. in draining lymph nodes, the anti-inflammatory effect of sge was manifested by the decrease of total ... | 2005 | 16475508 |
| an in vitro study of the activity of telithromycin against mobile and cystic forms of borrelia afzelii. | in this study the new ketolide telithromycin was tested in vitro against motile and cystic forms of borrelia afzelii, one of the species of borrelia burgdorferi sensu lato. acridine orange staining, dark field microscopy, and transmission electron microscopy were the techniques used to study the influence of telithromycin on the bacteria. the activity was unexpectedly high, 0.0003 microg/ml < mbc < or = 0.0006 microg/ml for the mobile forms after 7 days of incubation at 34 degrees c. mic was < 0 ... | 2006 | 16501899 |
| [erythema migrans due to borrelia afzelii]. | 2006 | 16510103 | |
| prevalence of borrelia burgdorferi sensu lato and anaplasmataceae members in ixodes ricinus ticks in alsace, a focus of lyme borreliosis endemicity in france. | due to the high lyme borreliosis incidence in alsace, in northeastern france, we investigated in 2003-2004 three cantons in this region in order to determine the density of ixodes ricinus ticks infected by borrelia burgdorferi sensu lato and anaplasmataceae. the peak density of nymphs infected by b. burgdorferi sensu lato at munster and guebwiller, where the disease incidence was high, was among the highest reported in europe (105 and 114 per 100 m(2), respectively). in contrast, the peak densit ... | 2006 | 16598024 |
| preparation and purification of recombinant outer surface protein a (rospa) of borrelia burgdorferi sensu stricto and borrelia afzelii. | the recombinant outer surface protein a (rospa) from borrelia burgdorferi is a possible immunogen for protection of infected humans and animals against development of lyme borreliosis (lyme disease), a chronic tick-borne disease characterised by diverse dermatologic, neurologic, rheumatic, and cardiac manifestations. for several years, research and development have been directed towards a vaccine for the prevention of this debilitating disease. numerous animal studies demonstrate that pre-existi ... | 2005 | 16601765 |
| borrelia lusitaniae and spotted fever group rickettsiae in ixodes ricinus (acari: ixodidae) in tuscany, central italy. | prevalence of infection by borrelia burgdorferi s.l. and spotted fever group (sfg) rickettsiae was estimated in host-seeking ticks in an area in tuscany, central italy, where lyme borreliosis was reported in a forestry worker. b. burgdorferi s.l. was identified by polymerase chain reaction in 16.7% (95% ci = 10.3, 24.8) of ixodes ricinus (l.) nymphs and 39.6% (95% ci = 26.5, 54.0) of adults. borrelia lusitaniae accounted for 82.9% of positive samples, followed by borrelia garinii (9.8%), borreli ... | 2006 | 16619594 |
| [allele variants of borrelia afzelii found by sequencing of the chromosome gene p66]. | sequencing of gene p66 fragments of 246-337 p.b. was performed in 45 isolates of aorrelia afzelii isolated from different transmitting agents and reservoir hosts within the habitation area of the spirochaeta. at least seven allele variants of the pathogenic agent were found indifferent natural foci of the disease. the extent of similarity between the nucleotide sequences of the isolates of the same allele variant was 99.9-100%; the extent of similarity between different allele variants was 98.9- ... | 2006 | 16756001 |
| enhanced culture of borrelia garinii and borrelia afzelii strains on a solid bsk-based medium in anaerobic conditions. | the growth of 29 human strains from the three main pathogenic species of borrelia burgdorferi sensu lato on a solid bsk-based medium was compared in two culture atmospheres: 3% co(2) air and anaerobiosis. all strains grew under anaerobic conditions, whereas only 13 strains were able to grow in aerobiosis with 3% co(2) (p<0.001). in the latter condition, 75% of the b. burgdorferi sensu stricto strains grew versus 33% of the b. garinii and b. afzelii strains. these data suggest that, especially fo ... | 2006 | 16814991 |
| perpetuation of the lyme disease spirochete borrelia lusitaniae by lizards. | to determine whether the lyme disease spirochete borrelia lusitaniae is associated with lizards, we compared the prevalence and genospecies of spirochetes present in rodent- and lizard-associated ticks at a site where this spirochete frequently infects questing ticks. whereas questing nymphal ixodes ricinus ticks were infected mainly by borrelia afzelii, one-half of the infected adult ticks harbored b. lusitaniae at our study site. lyme disease spirochetes were more prevalent in sand lizards (la ... | 2006 | 16820453 |
| [genetic heterogeneity of borrelia afzelii in the natural focus of the middle urals]. | as shown by sequencing the spacer rrf (5s)--rrl (23s) in 72 isolates of b. afzelii (one of the causative agents of ixodes tick borne borrelia infections) and the chromosomal gene coding protein p66 in 22 isolates, that in the natural focus located in the middle urals two different genetic subgroups (vs461 and nt28) of this genospecies simultaneously circulate. these subgroups are represented by 5 gene variants (rrf) 5s--(rrl) 23s and 5 allelic variants in gene p66. the latter, similarly to space ... | 2006 | 16830585 |
| a novel lymphocyte transformation test (ltt-melisa) for lyme borreliosis. | diagnosis of active lyme borreliosis (lb) remains a challenge in clinically ambiguous, serologically indeterminant, and polymerase chain reaction-negative patients. lymphocyte transformation tests (ltts) have been applied to detect specific cellular immune reactivity, but their clinical application has been severely hampered by the poorly defined borrelia antigens and nonstandardized ltt formats used. in this study, we describe the development and clinical relevance of a novel ltt using a valida ... | 2007 | 16876371 |
| evaluation of an internally controlled real-time pcr targeting the ospa gene for detection of borrelia burgdorferi sensu lato dna in cerebrospinal fluid. | this study reports the development and evaluation of an internally controlled real-time pcr targeting the ospa gene for detection of borrelia burgdorferi sensu stricto, borrelia garinii, borrelia afzelii and borrelia valaisiana. dna was extracted using qiaamp dna blood mini kit columns. dna from 33 b. burgdorferi sensu lato strains reacted in the assay, whereas no reactivity was observed with dna from four relapsing fever borrelia spp., 11 unrelated spirochaetes, and 31 unrelated microorganisms. ... | 2006 | 16882295 |
| comparison of findings for patients with borrelia garinii and borrelia afzelii isolated from cerebrospinal fluid. | the most common cause of lyme neuroborreliosis in europe is borrelia garinii, followed by borrelia afzelii. however, no series describing patients with culture-confirmed cases of lyme neuroborreliosis have been published, and no comparison of findings for patients with b. garinii and b. afzelii isolated from cerebrospinal fluid (csf) has been reported. | 2006 | 16912943 |
| borrelia afzelii gene expression in ixodes ricinus (acari: ixodidae) ticks. | spirochetes belonging to the borrelia burgdorferi sensu lato (s. l.) complex have evolved remarkable ability to survive in diverse ecological niches during transmission cycles between ticks and vertebrate hosts by variable gene expression. to understand the events during spirochete transmission from feeding ticks to hosts, mrna levels of selected b. afzelii genes (bbk32, dbpa, ospa, ospc and vlse) were measured by quantitative real-time sybr green pcr. b. afzelii infected ixodes ricinus nymphs f ... | 2006 | 16989569 |
| clinical appearance of erythema migrans caused by borrelia afzelii and borrelia garinii--effect of the patient's sex. | the aim in this survey was to study the clinical characteristics of infections caused by borrelia genospecies in patients with erythema migrans where borrelial origin was confirmed by polymerase chain reaction. the aim was also to study factors influencing the clinical appearance of erythema migrans. | 2006 | 17009065 |
| antigenicity of borrelial protein bbk32 fragments in early lyme borreliosis. | recombinantly produced borrelial bbk32 protein fragments originating from borrelia burgdorferi sensu stricto, borrelia garinii and borrelia afzelii were evaluated as antigens in the serology of lyme borreliosis (lb). in elisa, a mid-portion hydrophilic fragment reacted with lb patient sera. of the 23 patients with culture- or pcr-positive erythema migrans (em), 43 % at diagnosis and 52 % at convalescence were positive for at least one borrelia species-specific variant bbk32 fragment antigen. in ... | 2006 | 17030908 |
| use of cfse staining of borreliae in studies on the interaction between borreliae and human neutrophils. | species of the tick-transmitted spirochete group borrelia burgdorferi sensu lato (b. burgdorferi) cause lyme borreliosis. acute borrelial infection of the skin has unusual characteristics with only a mild local inflammatory response suggesting that the interaction between borreliae and the cells of the first-line defence might differ from that of other bacteria. it has been reported that human neutrophils phagocytose motile borreliae through an unconventional mechanism (tube phagocytosis) which ... | 2006 | 17049082 |
| canine borreliosis: a laboratory diagnostic trial. | the aim of this study was to investigate samples from dogs suggestive of active canine borreliosis (group a) by culture and pcr and the detection of antibodies against borrelia burgdorferi sensu lato in order to confirm a presumptive clinical diagnosis of canine borreliosis by laboratory results. criteria for such a diagnosis were: history of tick exposure, lameness, neurological signs, nephropathy, lethargy, anorexia, and fever. a total of 302 samples comprising edta blood, urine, synovial flui ... | 2007 | 17101241 |
| epidemiological characteristics and clinical manifestations of lyme borreliosis in croatia. | the goal was to show the epidemiological characteristics and clinical manifestations of lyme borreliosis in croatia. | 2006 | 17153550 |
| effect of tick saliva on mechanisms of innate immune response against borrelia afzelii. | the effect of salivary gland extract (sge) and saliva from the tick ixodes ricinus (l.) on the interaction of borrelia afzelii spirochetes with mouse macrophages as well as on the borreli-acidal effect of calf serum was studied. sge reduced both the number of phagocytosing cells and phagocytosed bacteria. an inhibitory effect of sge on the killing of spirochetes by the alternative pathway of complement activation also was observed. both sge and saliva down-regulated production of proinflammatory ... | 2006 | 17162955 |
| the use of local isolates in western blots improves serological diagnosis of lyme disease in scotland. | nine scottish borrelia burgdorferi isolates were investigated in igg western blot tests. sera previously found to be positive and negative when tested by routine western blots prepared from reference strain b. burgdorferi sensu stricto antigen had different outcomes with these isolates. two isolates, e5 (borrelia afzelii) and g4 (b. burgdorferi sensu stricto) performed well, reproducing western blot-positive results in 90 and 95% of tests, respectively. when antigens from both isolates were inco ... | 2007 | 17172516 |
| cutaneous lymphoid hyperplasia presenting as a solitary facial nodule: clinical, histopathological, immunophenotypical, and molecular studies. | to elucidate the clinicopathological, immunophenotypical, and molecular characteristics of cutaneous lymphoid hyperplasia presenting as a solitary facial nodule. | 2006 | 17178981 |
| seasonal variations in detecting borrelia burgdorferi sensu lato in rodents from north eastern austria. | austria is well known as an endemic area of lyme borreliosis. to assess the annual variation of rodent populations that may host agents of lyme borreliosis we collected rodents in northeastern austria. life traps were set out every six weeks during a year consecutively in one each of the three different zones (hohenau, ernstbrunn, vienna woods) that cover the main habitat characteristics of small mammals in northeastern austria. rodents were collected and identified. samples of heart, urine blad ... | 2006 | 17186171 |
| ticks (ixodidae) from passerine birds in the carpathian region. | birds have been found to be a reservoir host of borrelia. in order to assess the situation in slovakia ticks were collected from a total of 3057 mist-netted, ringed and released passerine birds in two locations at 500 m (in 2001) and 1000 m (in 2003) above sea level in the bukovské vrchy hills, part of the carpathian region in the north-east of slovakia. a total of 75 birds of 16 species were infested with subadult ticks of ixodes ricinus species (prevalence of parasitization 5%). sixty-two larv ... | 2006 | 17186172 |
| pathogens and host dna in ixodes ricinus nymphal ticks from a german forest. | unfed nymphs of ixodes ricinus were collected from vegetation in a forest on the outskirts of berlin, germany and were analyzed for host and pathogen dna. pathogens were detected in 47% of the ticks. borrelia afzelii was the commonest pathogen detected, followed by rickettsia helvetica. other pathogens included b. valaisiana, b. garinii, b. burgdorferi sensu stricto, anaplasma phagocytophilum, and a relapsing fever-like borrelia. most of the host dna detected was of rodent origin and was associa ... | 2006 | 17187573 |
| prevalence of borrelia burgdorferi genospecies in ixodes ricinus ticks from lublin region (eastern poland). | the objective of the study was to determine the prevalence rate of 3 borrelia burgdorferi genospecies in ixodes ricinus ticks collected from wooded areas of the lublin region (eastern poland). a group of 1,813 i. ricinus ticks from 6 districts were examined for the presence of borrelia burgdorferi sensu lato (b.b.s.l.) by polymerase chain reaction (pcr). another group of 438 i. ricinus ticks collected from 4 districts were examined for the presence of b.b.s.l. by culture on bskh liquid medium co ... | 2006 | 17196005 |
| isolation of borrelia afzelii from overwintering culex pipiens biotype molestus mosquitoes. | a total of 662 samples (winter period: 469; summer period: 193 specimens) of female mosquitoes of the genus culex, aedes and anopheles were collected during the period march 2000-april 2001 from the locality of vysoke myto (eastern bohemia, czech republic). they were examined by dark field microscopy for the presence of spirochetes. the motile spirochetes were observed in 4.2 % of all species of investigated mosquitoes. one spirochetal strain out of the 8 isolation attempts (brz14) was obtained ... | 2006 | 17199258 |
| complementation of a borrelia afzelii ospc mutant highlights the crucial role of ospc for dissemination of borrelia afzelii in ixodes ricinus. | alteration of the outer surface protein (osp) composition--especially that of ospa and ospc--seems to be important for the adaptation of borrelia burgdorferi sensu lato to its endothermic hosts (mammals) and poikilothermic vectors (ticks). ospa possibly mediates adherence to tick midgut cells thus enabling the borreliae to survive in the vector, while ospc is associated with borrelial invasion of the tick salivary glands and infection of the mammalian hosts. here we describe the first successful ... | 2007 | 17267282 |
| [detection of dna of borrelia circulating in novosibirsk region]. | detection of dna of borrelia burgdorferi sensu lato was performed by pcr in taiga ticks ixodes persulcatus, in blood samples and skin bioptates of small forest mammals, and in blood and urine samples of humans after attaching of ticks events. in novosibirsk region both in natural reservoir and in patients with ixodes ticks-borne borreliosis dna of borrelia garinii and borrelia afzelii are detected. dna of these borrelia were detected in 8 from 72 of taiga ticks, in 36 from 298 of blood and skin ... | 2006 | 17297875 |
| clinical features and specific immunological response to borrelia afzelii in patients with acrodermatitis chronica atrophicans. | acrodermatitis chronica atrophicans (aca) occurs mostly in europe. borrelia afzelii is considered to be responsible for this manifestation of lyme borreliosis. the aim of the study was to observe the clinical features of the aca and evaluate the specific immunological response to borrelia afzelii. nine patients from an endemic lyme borreliosis region in northeastern poland were studied. the serum samples were tested routinely with ifa and eia and, following testing, with immunoblots using borrel ... | 1999 | 17312680 |
| effect of gender on clinical and epidemiologic features of lyme borreliosis. | the aim is to highlight the influence of patients' gender on lyme borreliosis and especially erythema migrans (em), focusing on exposure to tick bites, epidemiology, and the clinical picture. all studies were conducted in the county of blekinge, located in southeastern sweden. a prospective study was conducted in 235 individuals (women, n=110; men, n=125) engaged in recreational or occupational activities focusing on exposure to tick bites. a retrospective epidemiologic study evaluating 123,495 ... | 2007 | 17417955 |
| in vitro activities of faropenem, ertapenem, imipenem and meropenem against borrelia burgdorferi s.l. | little is known about the in vitro activity of penems and carbapenems against the spirochete borrelia burgdorferi. here, faropenem, ertapenem, imipenem and meropenem as well as the third-generation cephalosporin ceftriaxone and tobramycin were tested in vitro against 11 isolates of the b. burgdorferi sensu lato complex. on a microg/ml basis, ertapenem was the most potent carbapenem (minimal inhibitory concentration (mic) range: 0.015-0.125 microg/ml), with in vitro activity comparable with that ... | 2007 | 17512703 |
| tick-borne zoonotic bacteria in wild and domestic small mammals in northern spain. | the prevalence and diversity of tick-borne zoonotic bacteria (borrelia spp., anaplasma phagocytophilum, coxiella burnetii, and spotted fever group rickettsiae) infecting 253 small mammals captured in the basque country (spain) were assessed using pcr and reverse line blot hybridization. trapping sites were selected around sheep farms (study 1, 2000 to 2002) and recreational parks (study 2, 2003 to 2005). the majority of the studied mammals (162) were wood mice (apodemus sylvaticus), but six othe ... | 2007 | 17693556 |
| [concentration of sfas and sfasl in the supernatant of pbmc culture from the patients with late lyme borreliosis]. | persistent, inadequate inflammatory response present in late lyme borreliosis may be driven by activated t lymphocytes. we estimated synthesis of extracellular proteins: soluble fas receptor (sfas) and its ligand (sfasl), which might protect t lymphocytes from physiologic apoptosis, in the peripheral blood mononuclear cell (pbmc) culture from patients with late borreliosis. | 2007 | 17702439 |
| early disseminated borreliosis with multiple erythema migrans and elevated liver enzymes: case report and literature review. | a 69-year-old man presented with multiple livid maculae and infiltrated urticarial plaques, as well as elevated liver enzymes. based on typical clinical picture, histopathology and positive pcr from a skin biopsy, we diagnosed an early disseminated infection with borrelia afzelii presenting with multiple erythema migrans (erythemata migrantia) and a subclinical hepatitis. during antibiotic treatment with intravenous ceftriaxone, the maculae and plaques vanished almost completely and the liver en ... | 2007 | 17721649 |
| first molecular detection of borrelia afzelii in clinical samples in korea. | borrelia afzelii nucleic acids were detected in the sera of febrile disease patients by a nested pcr that targeted the rrf (5s)-rrl (23s) spacer of b. burgdorferi sensu lato. the b. afzelii-specific dna fragment was detected in 8 out of 283 sera which were proven to have immunoglobulin g or m antibodies against b. burgdorferi antigens through ifa. the results were further confirmed through restriction fragment length polymorphism and sequencing analysis of the dna fragments. the results indicate ... | 2007 | 18094538 |
| higher mrna levels of chemokines and cytokines associated with macrophage activation in erythema migrans skin lesions in patients from the united states than in patients from austria with lyme borreliosis. | erythema migrans (em) is caused primarily by borrelia afzelii in europe and solely by borrelia burgdorferi in the united states. b. burgdorferi infection in the united states has previously been associated with faster expansion of em lesions and with more associated symptoms, compared with b. afzelii infection in europe. however, reasons for these differences are not yet known. | 2008 | 18171218 |
| concentration of tgf-beta1 in the supernatant of peripheral blood mononuclear cells cultures from patients with early disseminated and chronic lyme borreliosis. | the aberrant inflammatory response is probably involved in the pathogenesis of chronic lyme borreliosis, including chronic lyme arthritis and neuroborreliosis. transforming growth factor-beta 1 (tgf-beta1) is an important anti-inflammatory and immunomodulatory cytokine and its deficient synthesis is linked to exaggerated inflammation and immune response. | 2007 | 18217413 |
| prevalence of babesia canis, borrelia afzelii, and anaplasma phagocytophilum infection in hard ticks removed from dogs in warsaw (central poland). | the purposes of this study were to specify the occurrence and prevalence of babesia canis, borrelia burgdorferi sensu lato, and anaplasma phagocytophilum in ticks removed from dogs in warsaw, and to determine the borrelia species occurring in ixodes ricinus ticks. among 590 collected ticks, 209 were identified as i. ricinus, and 381 as dermacentor reticulatus. dna of b. canis was detected in 11% of d. reticulatus ticks. we found that 6.2% of i. ricinus ticks harbored b. burgdorferi s.l. specific ... | 2008 | 18328630 |
| diversity of ixodes ricinus tick-associated bacterial communities from different forests. | nymphal ixodes ricinus ticks (n=180) were collected from three different areas in the netherlands to investigate the effect of forest composition on tick-associated microbial communities. sampled habitats differed in thickness of leaf litter and humus layers and vegetation associations and were located near amsterdam (beech-oak), ede (birch-oak) and veldhoven (birch-oak). analysis of nine 16s rrna gene clone libraries made from individual ticks showed nearest matches with presumed pathogens cand ... | 2008 | 18355299 |
| detection of borrelia burgdorferi sensu lato dna by pcr in serum of patients with clinical symptoms of lyme borreliosis. | lyme borreliosis is a disease caused by spirochaetes belonging to the genospecies complex borrelia burgdorferi sensu lato (s.l.) transmitted by ixodes ticks. at present, serology remains the main diagnostic tool for laboratory diagnosis of lyme borreliosis. recently, the pcr technique has been applied for diagnosis of b. burgdorferi s.l., but, until now, a reliable, easy-to-perform and sensitive method has not been described. here we present a new pcr-based method for the detection of both b. bu ... | 2008 | 18384365 |
| borrelia bissettii isolates induce pathology in a murine model of disease. | the spirochete borrelia burgdorferi is a tick-borne pathogen that causes lyme disease. although b. burgdorferi sensu lato is a diverse group of bacteria, only three genospecies, b. burgdorferi sensu stricto, borrelia afzelii, and borrelia garinii, are known to be pathogenic and commonly recognized to cause human disease. to assess the potential of another common genospecies, borrelia bissettii, to induce disease, a mouse model was employed. two colorado isolates of b. bissettii (co-bb) induced l ... | 2008 | 18454594 |
| molecular and serological evidence of borrelia burgdorferi sensu lato in wild rodents in the czech republic. | the aim of the present study was to determine the frequency and spatial distribution of the borrelia species in wild rodents in the czech republic. in total, 293 muscle tissue samples and 106 sera from 293 wild rodents captured in north bohemia and north-east and south moravia were examined for the presence of borrelia spp. and antibodies. muscle samples were investigated with real-time polymerase chain reaction (pcr) with a reca primer set, with dna quantification and melting curve analysis, an ... | 2008 | 18454596 |
| [activity of the caspase-3 in the culture of peripheral blood mononuclear cells stimulated with borrelia burgdorferi antigens]. | to investigate whether the decreased rate of apoptosis of peripheral blood mononuclear cells occurs in patients with chronic lyme arthritis (la), which could contribute to persistent inflammation in those patients. | 2008 | 18536229 |
| improved laboratory diagnostics of lyme neuroborreliosis in children by detection of antibodies to new antigens in cerebrospinal fluid. | laboratory diagnostics in lyme neuroborreliosis need improvement. we hereby investigate 4 new recombinant or peptide borrelia antigens in cerebrospinal fluid in children with neuroborreliosis to evaluate their performance as diagnostic antigens. | 2008 | 18536620 |
| comparison of pcr methods and culture for the detection of borrelia spp. in patients with erythema migrans. | the sensitivities of two pcr assays and culture were compared for the detection of borrelia spp. in skin specimens of 150 patients with typical erythema migrans. in addition, the accuracy of the methods for the identification of borrelia spp. was compared by analysing culture isolates and material obtained directly from skin biopsy specimens. borrelia burgdorferi sensu lato was isolated from 73 (49%) of 150 skin biopsy specimens. using a nested pcr targeting the rrf-rrl region and a pcr targetin ... | 2008 | 18558937 |
| characterization of borrelia burgdorferi sensu lato isolates by pulsed-field gel electrophoresis after mlui restriction of genomic dna. | at least three borrelia species (borrelia afzelii, borrelia garinii and borrelia burgdorferi sensu stricto) cause disease in humans, but borrelia spielmanii, borrelia valaisiana, borrelia lusitaniae and borrelia bissettii have also been reported to be rare or potential causes of human disease in europe. pulsed-field gel electrophoresis after mlui restriction of the genomic dna (mlui large restriction fragment patterns, lrfps) represents one of several approaches that have been used to assess bor ... | 2008 | 18586084 |
| presence of pathogenic borrelia burgdorferi sensu lato in ticks and rodents in zhejiang, south-east china. | a molecular epidemiological survey was conducted to investigate the presence of pathogenic borrelia burgdorferi sensu lato (s.l.) species in the forest areas of zhejiang province, south-east china. a total of 182 ticks of 6 species and 200 rodents of 8 species were collected and individually examined for the presence of b. burgdorferi s.l. dna by nested pcr targeting the 5s-23s rrna intergenic spacer. forty-one ticks of four species, haemaphysalis concinna, haemaphysalis longicornis, rhipicephal ... | 2008 | 18628499 |
| prevalence of anaplasma phagocytophilum and its coinfection with borrelia afzelii in ixodes ricinus and ixodes persulcatus ticks inhabiting tver province (russia) - a sympatric region for both tick species. | human granulocytic anaplasmosis (hga) and lyme borreliosis (lb) are tick-borne infectious diseases caused by anaplasma phagocytophilum and borrelia burgdorferi sensu lato species, respectively. in this study, p44/msp2 paralogues specific to a. phagocytophilum and 5s-23s rrna gene-intergenic spacers specific to b. burgdorferi sensu lato species were detected by pcr in ticks collected in two regions, tver (kalinin) and konakovo, of the tver (kalinin) province located 150 km north-west of moscow. t ... | 2008 | 18628500 |
| validation of cultivation and pcr methods for diagnosis of lyme neuroborreliosis. | borrelial infection may manifest with a wide range of clinical signs, and in many cases, microbiological findings are essential for a proper diagnosis. this study included 48 patients with a working clinical diagnosis of lyme neuroborreliosis, 45 patients with a working clinical diagnosis of suspected lyme neuroborreliosis, and a control group comprising 42 patients with tick-borne encephalitis and 21 neurosurgical patients. the aim of the study was to analyze and compare findings of two pcr met ... | 2008 | 18716226 |
| differential role of passerine birds in distribution of borrelia spirochetes, based on data from ticks collected from birds during the postbreeding migration period in central europe. | borrelia spirochetes in bird-feeding ticks were studied in the czech republic. during the postbreeding period (july to september 2005), 1,080 passerine birds infested by 2,240 ixodes ricinus subadult ticks were examined. borrelia garinii was detected in 22.2% of the ticks, borrelia valaisiana was detected in 12.8% of the ticks, borrelia afzelii was detected in 1.6% of the ticks, and borrelia burgdorferi sensu stricto was detected in 0.3% of the ticks. after analysis of infections in which the bl ... | 2009 | 19060160 |