Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| [concentration of sfas and sfasl in the supernatant of pbmc culture from the patients with late lyme borreliosis]. | persistent, inadequate inflammatory response present in late lyme borreliosis may be driven by activated t lymphocytes. we estimated synthesis of extracellular proteins: soluble fas receptor (sfas) and its ligand (sfasl), which might protect t lymphocytes from physiologic apoptosis, in the peripheral blood mononuclear cell (pbmc) culture from patients with late borreliosis. | 2007 | 17702439 |
| first molecular detection of borrelia afzelii in clinical samples in korea. | borrelia afzelii nucleic acids were detected in the sera of febrile disease patients by a nested pcr that targeted the rrf (5s)-rrl (23s) spacer of b. burgdorferi sensu lato. the b. afzelii-specific dna fragment was detected in 8 out of 283 sera which were proven to have immunoglobulin g or m antibodies against b. burgdorferi antigens through ifa. the results were further confirmed through restriction fragment length polymorphism and sequencing analysis of the dna fragments. the results indicate ... | 2007 | 18094538 |
| [detection of dna of borrelia circulating in novosibirsk region]. | detection of dna of borrelia burgdorferi sensu lato was performed by pcr in taiga ticks ixodes persulcatus, in blood samples and skin bioptates of small forest mammals, and in blood and urine samples of humans after attaching of ticks events. in novosibirsk region both in natural reservoir and in patients with ixodes ticks-borne borreliosis dna of borrelia garinii and borrelia afzelii are detected. dna of these borrelia were detected in 8 from 72 of taiga ticks, in 36 from 298 of blood and skin ... | 2006 | 17297875 |
| [genetic heterogeneity of borrelia afzelii in the natural focus of the middle urals]. | as shown by sequencing the spacer rrf (5s)--rrl (23s) in 72 isolates of b. afzelii (one of the causative agents of ixodes tick borne borrelia infections) and the chromosomal gene coding protein p66 in 22 isolates, that in the natural focus located in the middle urals two different genetic subgroups (vs461 and nt28) of this genospecies simultaneously circulate. these subgroups are represented by 5 gene variants (rrf) 5s--(rrl) 23s and 5 allelic variants in gene p66. the latter, similarly to space ... | 2006 | 16830585 |
| [allele variants of borrelia afzelii found by sequencing of the chromosome gene p66]. | sequencing of gene p66 fragments of 246-337 p.b. was performed in 45 isolates of aorrelia afzelii isolated from different transmitting agents and reservoir hosts within the habitation area of the spirochaeta. at least seven allele variants of the pathogenic agent were found indifferent natural foci of the disease. the extent of similarity between the nucleotide sequences of the isolates of the same allele variant was 99.9-100%; the extent of similarity between different allele variants was 98.9- ... | 2006 | 16756001 |
| clinical appearance of erythema migrans caused by borrelia afzelii and borrelia garinii--effect of the patient's sex. | the aim in this survey was to study the clinical characteristics of infections caused by borrelia genospecies in patients with erythema migrans where borrelial origin was confirmed by polymerase chain reaction. the aim was also to study factors influencing the clinical appearance of erythema migrans. | 2006 | 17009065 |
| comparison of findings for patients with borrelia garinii and borrelia afzelii isolated from cerebrospinal fluid. | the most common cause of lyme neuroborreliosis in europe is borrelia garinii, followed by borrelia afzelii. however, no series describing patients with culture-confirmed cases of lyme neuroborreliosis have been published, and no comparison of findings for patients with b. garinii and b. afzelii isolated from cerebrospinal fluid (csf) has been reported. | 2006 | 16912943 |
| cutaneous lymphoid hyperplasia presenting as a solitary facial nodule: clinical, histopathological, immunophenotypical, and molecular studies. | to elucidate the clinicopathological, immunophenotypical, and molecular characteristics of cutaneous lymphoid hyperplasia presenting as a solitary facial nodule. | 2006 | 17178981 |
| seasonal variations in detecting borrelia burgdorferi sensu lato in rodents from north eastern austria. | austria is well known as an endemic area of lyme borreliosis. to assess the annual variation of rodent populations that may host agents of lyme borreliosis we collected rodents in northeastern austria. life traps were set out every six weeks during a year consecutively in one each of the three different zones (hohenau, ernstbrunn, vienna woods) that cover the main habitat characteristics of small mammals in northeastern austria. rodents were collected and identified. samples of heart, urine blad ... | 2006 | 17186171 |
| ticks (ixodidae) from passerine birds in the carpathian region. | birds have been found to be a reservoir host of borrelia. in order to assess the situation in slovakia ticks were collected from a total of 3057 mist-netted, ringed and released passerine birds in two locations at 500 m (in 2001) and 1000 m (in 2003) above sea level in the bukovské vrchy hills, part of the carpathian region in the north-east of slovakia. a total of 75 birds of 16 species were infested with subadult ticks of ixodes ricinus species (prevalence of parasitization 5%). sixty-two larv ... | 2006 | 17186172 |
| pathogens and host dna in ixodes ricinus nymphal ticks from a german forest. | unfed nymphs of ixodes ricinus were collected from vegetation in a forest on the outskirts of berlin, germany and were analyzed for host and pathogen dna. pathogens were detected in 47% of the ticks. borrelia afzelii was the commonest pathogen detected, followed by rickettsia helvetica. other pathogens included b. valaisiana, b. garinii, b. burgdorferi sensu stricto, anaplasma phagocytophilum, and a relapsing fever-like borrelia. most of the host dna detected was of rodent origin and was associa ... | 2006 | 17187573 |
| prevalence of borrelia burgdorferi genospecies in ixodes ricinus ticks from lublin region (eastern poland). | the objective of the study was to determine the prevalence rate of 3 borrelia burgdorferi genospecies in ixodes ricinus ticks collected from wooded areas of the lublin region (eastern poland). a group of 1,813 i. ricinus ticks from 6 districts were examined for the presence of borrelia burgdorferi sensu lato (b.b.s.l.) by polymerase chain reaction (pcr). another group of 438 i. ricinus ticks collected from 4 districts were examined for the presence of b.b.s.l. by culture on bskh liquid medium co ... | 2006 | 17196005 |
| isolation of borrelia afzelii from overwintering culex pipiens biotype molestus mosquitoes. | a total of 662 samples (winter period: 469; summer period: 193 specimens) of female mosquitoes of the genus culex, aedes and anopheles were collected during the period march 2000-april 2001 from the locality of vysoke myto (eastern bohemia, czech republic). they were examined by dark field microscopy for the presence of spirochetes. the motile spirochetes were observed in 4.2 % of all species of investigated mosquitoes. one spirochetal strain out of the 8 isolation attempts (brz14) was obtained ... | 2006 | 17199258 |
| epidemiological characteristics and clinical manifestations of lyme borreliosis in croatia. | the goal was to show the epidemiological characteristics and clinical manifestations of lyme borreliosis in croatia. | 2006 | 17153550 |
| effect of tick saliva on mechanisms of innate immune response against borrelia afzelii. | the effect of salivary gland extract (sge) and saliva from the tick ixodes ricinus (l.) on the interaction of borrelia afzelii spirochetes with mouse macrophages as well as on the borreli-acidal effect of calf serum was studied. sge reduced both the number of phagocytosing cells and phagocytosed bacteria. an inhibitory effect of sge on the killing of spirochetes by the alternative pathway of complement activation also was observed. both sge and saliva down-regulated production of proinflammatory ... | 2006 | 17162955 |
| serological confirmation of borrelia burgdorferi infection in dogs in the czech republic. | from the epidemiological point of view, dogs are very important since they are considered a suitable indicator of the spread of human borreliosis. serum samples obtained from healthy, asymptomatic military dogs from 12 different areas in the czech republic were examined for igg antibodies to borrelia burgdorferi sensu lato (s.l.). the total of 399 serum samples were tested by a whole-cell elisa. specific antibodies to borrelia burgdorferi s.l. were detected in 26 cases (6.5%). in different local ... | 2006 | 16437298 |
| an in vitro study of the activity of telithromycin against mobile and cystic forms of borrelia afzelii. | in this study the new ketolide telithromycin was tested in vitro against motile and cystic forms of borrelia afzelii, one of the species of borrelia burgdorferi sensu lato. acridine orange staining, dark field microscopy, and transmission electron microscopy were the techniques used to study the influence of telithromycin on the bacteria. the activity was unexpectedly high, 0.0003 microg/ml < mbc < or = 0.0006 microg/ml for the mobile forms after 7 days of incubation at 34 degrees c. mic was < 0 ... | 2006 | 16501899 |
| [erythema migrans due to borrelia afzelii]. | 2006 | 16510103 | |
| prevalence of borrelia burgdorferi sensu lato and anaplasmataceae members in ixodes ricinus ticks in alsace, a focus of lyme borreliosis endemicity in france. | due to the high lyme borreliosis incidence in alsace, in northeastern france, we investigated in 2003-2004 three cantons in this region in order to determine the density of ixodes ricinus ticks infected by borrelia burgdorferi sensu lato and anaplasmataceae. the peak density of nymphs infected by b. burgdorferi sensu lato at munster and guebwiller, where the disease incidence was high, was among the highest reported in europe (105 and 114 per 100 m(2), respectively). in contrast, the peak densit ... | 2006 | 16598024 |
| borrelia lusitaniae and spotted fever group rickettsiae in ixodes ricinus (acari: ixodidae) in tuscany, central italy. | prevalence of infection by borrelia burgdorferi s.l. and spotted fever group (sfg) rickettsiae was estimated in host-seeking ticks in an area in tuscany, central italy, where lyme borreliosis was reported in a forestry worker. b. burgdorferi s.l. was identified by polymerase chain reaction in 16.7% (95% ci = 10.3, 24.8) of ixodes ricinus (l.) nymphs and 39.6% (95% ci = 26.5, 54.0) of adults. borrelia lusitaniae accounted for 82.9% of positive samples, followed by borrelia garinii (9.8%), borreli ... | 2006 | 16619594 |
| enhanced culture of borrelia garinii and borrelia afzelii strains on a solid bsk-based medium in anaerobic conditions. | the growth of 29 human strains from the three main pathogenic species of borrelia burgdorferi sensu lato on a solid bsk-based medium was compared in two culture atmospheres: 3% co(2) air and anaerobiosis. all strains grew under anaerobic conditions, whereas only 13 strains were able to grow in aerobiosis with 3% co(2) (p<0.001). in the latter condition, 75% of the b. burgdorferi sensu stricto strains grew versus 33% of the b. garinii and b. afzelii strains. these data suggest that, especially fo ... | 2006 | 16814991 |
| perpetuation of the lyme disease spirochete borrelia lusitaniae by lizards. | to determine whether the lyme disease spirochete borrelia lusitaniae is associated with lizards, we compared the prevalence and genospecies of spirochetes present in rodent- and lizard-associated ticks at a site where this spirochete frequently infects questing ticks. whereas questing nymphal ixodes ricinus ticks were infected mainly by borrelia afzelii, one-half of the infected adult ticks harbored b. lusitaniae at our study site. lyme disease spirochetes were more prevalent in sand lizards (la ... | 2006 | 16820453 |
| evaluation of an internally controlled real-time pcr targeting the ospa gene for detection of borrelia burgdorferi sensu lato dna in cerebrospinal fluid. | this study reports the development and evaluation of an internally controlled real-time pcr targeting the ospa gene for detection of borrelia burgdorferi sensu stricto, borrelia garinii, borrelia afzelii and borrelia valaisiana. dna was extracted using qiaamp dna blood mini kit columns. dna from 33 b. burgdorferi sensu lato strains reacted in the assay, whereas no reactivity was observed with dna from four relapsing fever borrelia spp., 11 unrelated spirochaetes, and 31 unrelated microorganisms. ... | 2006 | 16882295 |
| borrelia afzelii gene expression in ixodes ricinus (acari: ixodidae) ticks. | spirochetes belonging to the borrelia burgdorferi sensu lato (s. l.) complex have evolved remarkable ability to survive in diverse ecological niches during transmission cycles between ticks and vertebrate hosts by variable gene expression. to understand the events during spirochete transmission from feeding ticks to hosts, mrna levels of selected b. afzelii genes (bbk32, dbpa, ospa, ospc and vlse) were measured by quantitative real-time sybr green pcr. b. afzelii infected ixodes ricinus nymphs f ... | 2006 | 16989569 |
| antigenicity of borrelial protein bbk32 fragments in early lyme borreliosis. | recombinantly produced borrelial bbk32 protein fragments originating from borrelia burgdorferi sensu stricto, borrelia garinii and borrelia afzelii were evaluated as antigens in the serology of lyme borreliosis (lb). in elisa, a mid-portion hydrophilic fragment reacted with lb patient sera. of the 23 patients with culture- or pcr-positive erythema migrans (em), 43 % at diagnosis and 52 % at convalescence were positive for at least one borrelia species-specific variant bbk32 fragment antigen. in ... | 2006 | 17030908 |
| use of cfse staining of borreliae in studies on the interaction between borreliae and human neutrophils. | species of the tick-transmitted spirochete group borrelia burgdorferi sensu lato (b. burgdorferi) cause lyme borreliosis. acute borrelial infection of the skin has unusual characteristics with only a mild local inflammatory response suggesting that the interaction between borreliae and the cells of the first-line defence might differ from that of other bacteria. it has been reported that human neutrophils phagocytose motile borreliae through an unconventional mechanism (tube phagocytosis) which ... | 2006 | 17049082 |
| preparation and purification of recombinant outer surface protein a (rospa) of borrelia burgdorferi sensu stricto and borrelia afzelii. | the recombinant outer surface protein a (rospa) from borrelia burgdorferi is a possible immunogen for protection of infected humans and animals against development of lyme borreliosis (lyme disease), a chronic tick-borne disease characterised by diverse dermatologic, neurologic, rheumatic, and cardiac manifestations. for several years, research and development have been directed towards a vaccine for the prevention of this debilitating disease. numerous animal studies demonstrate that pre-existi ... | 2005 | 16601765 |
| identification of a new borrelia species among small mammals in areas of northern spain where lyme disease is endemic. | the role of small mammals as reservoir hosts for borrelia burgdorferi was investigated in several areas where lyme disease is endemic in northern spain. a low rate of infestation by ixodes ricinus nymphs was found in the small mammal populations studied that correlated with the near-absence of b. burgdorferi sensu lato in 184 animals tested and with the lack of transmission of b. burgdorferi sensu lato to i. ricinus larvae that fed on them. in contrast, questing ticks collected at the same time ... | 2005 | 15746336 |
| improving the specificity of 16s rdna-based polymerase chain reaction for detecting borrelia burgdorferi sensu lato-causative agents of human lyme disease. | 16s rdna sequences of borrelia burgdorferi sensu lato were aligned with the 16s rdna sequences of borrelia hermsii, borrelia turicatae, and borrelia lonestari in order to identify primers that might be used to more specifically identify agents of human lyme disease in ticks in human skin samples. | 2005 | 15752343 |
| diversity of borrelia burgdorferi sensu lato in russian far east. | thirty strains of borrelia burgdorferi sensu lato have been isolated from ixodes persulcatus ticks and from skin lesions of lyme disease patients in the russian far east from 1997 to 2003. we amplified full-length outer surface protein a (ospa) gene of all strains. blast search and following phylogenetic analysis showed that strains form four well-defined groups. four strains belong to borrelia afzelii species. other strains distributed into tree major groups, identified as borrelia garinii. ind ... | 2005 | 15781992 |
| identification and functional characterization of complement regulator-acquiring surface protein 1 of the lyme disease spirochetes borrelia afzelii and borrelia garinii. | complement regulator-acquiring surface protein 1 (crasp-1) is the dominant factor-h-like protein 1 (fhl-1)- and factor-h-binding protein of borrelia burgdorferi and is suggested to contribute to persistence of the pathogen. the prototype crasp-1 of b. burgdorferi sensu stricto (crasp-1bb) has been formerly characterized. as shown recently, serum-resistant borrelia afzelii strains express a unique fhl-1 and factor h-binding protein, designated crasp-1ba. here, we describe for the first time the i ... | 2005 | 15784581 |
| in vitro susceptibility testing of borrelia burgdorferi sensu lato isolates cultured from patients with erythema migrans before and after antimicrobial chemotherapy. | clinical treatment failures have been reported to occur in early lyme borreliosis (lb) for many suitable antimicrobial agents. investigations of possible resistance mechanisms of the borrelia burgdorferi complex must analyze clinical isolates obtained from lb patients, despite their receiving antibiotic treatment. here, borrelial isolates obtained from five patients with erythema migrans (em) before the start of antibiotic therapy and again after the conclusion of treatment were investigated. th ... | 2005 | 15793100 |
| risk of tick-borne bacterial diseases among workers of roztocze national park (south-eastern poland). | the objective of the present study was to assess the risk of borreliosis and anaplasmosis (ehrlichiosis) among the forestry workers of the roztocze national park (south-eastern poland) by examination of ixodes ricinus ticks living in park area with pcr method, and by the serological and clinical examination of the workers. in 406 examined ticks, the prevalence of infection with borrelia burgdorferi sensu lato was 11.5 %. the nested pcr reaction for determining the genospecies showed that the mos ... | 2005 | 16028877 |
| solving a sticky problem: new genetic approaches to host cell adhesion by the lyme disease spirochete. | the lyme disease spirochetes, comprised of at least three closely related species, borrelia burgdorferi, borrelia garinii and borrelia afzelii, are fascinating and enigmatic bacterial pathogens. they are maintained by tick-mediated transmission between mammalian hosts, usually small rodents. the ability of these bacteria, which have relatively small genomes, to survive and disseminate in both an immunocompetent mammal and in an arthropod vector suggests that they have evolved elegant and indispe ... | 2005 | 16101994 |
| tickborne pathogen detection, western siberia, russia. | ixodes persulcatus (n = 125) and dermacentor reticulatus (n = 84) ticks from western siberia, russia, were tested for infection with borrelia, anaplasma/ehrlichia, bartonella, and babesia spp. by using nested polymerase chain reaction assays with subsequent sequencing. i. persulcatus ticks were infected with borrelia burgdorferi sensu lato (37.6% +/- 4.3% [standard deviation]), anaplasma phagocytophilum (2.4% +/- 1.4%), ehrlichia muris (8.8% +/- 2.5%), and bartonella spp. (37.6% +/- 4.3%). d. re ... | 2005 | 16318722 |
| hosts and pathogen detection for immature stages of ixodes ricinus (acari: ixodidae) in north-central spain. | to determine hosts of the immature stages of a southern population of ixodes ricinus, we trapped rodents and birds in an area of north-central spain in may-june and august-september of 1998 and 1999. the most frequently trapped rodents were apodemus sylvaticus (230 specimens) and clethrionomys glareolus (99), with a larval infestation prevalence of 49% and 81%, respectively (in spring) and 21% and 41% (in summer). c. glareolus was always more heavily parasitized by larvae (mean numbers 19.8 in s ... | 2005 | 16323055 |
| [lyme borreliosis--an overview]. | north american lyme disease and european borreliosis differ in many aspects: whereas in north america infection by borrelia burgdorferi sensu stricto almost invariably leads to erythema migrans, frequently followed by oligoarthritis in untreated individuals, most infections in europe are caused by borrelia garinii and borrelia afzelii and are contracted asymptomatically. a minority of the infected individuals develop neuroborreliosis, consisting of various combinations of meningitis, radiculitis ... | 2005 | 16350535 |
| lyme borreliosis: insights into tick-/host-borrelia relations. | lyme borreliosis (lb) is a serious infectious disease of humans and some domestic animals in temperate regions of the northern hemisphere. it is caused by certain spirochetes in the borrelia burgdorferi sensu lato (s.l.) species complex. the complex consists of 11 species (genospecies). borrelia burgdorferi sensu stricto (s.s.), borrelia garinii and borrelia afzelii are the major agents of human disease. borrelia burgdorferi s.l. species are transmitted mainly by ticks belonging to the ixodes ri ... | 2005 | 16405291 |
| co-inoculation of borrelia afzelii with tick salivary gland extract influences distribution of immunocompetent cells in the skin and lymph nodes of mice. | the impact of ixodes ricinus salivary gland extract (sge) on inflammatory changes in the skin and draining lymph nodes of mice, elicited by the infection with the important human pathogen, b. afzelii, was determined using flow cytometry. sge injected together with spirochetes reduced the numbers of leukocytes and gammadelta-t lymphocytes in infected epidermis at early time-points post infection. in draining lymph nodes, the anti-inflammatory effect of sge was manifested by the decrease of total ... | 2005 | 16475508 |
| characterization of borrelia burgdorferi sensu lato isolated in moscow province--a sympatric region for ixodes ricinus and ixodes persulcatus. | the relationships between borrelia species and the vector ticks, ixodes persulcatus and ixodes ricinus were examined in a molecular epidemiological study. we conducted a survey in the moscow region which is a sympatric region for both species of tick. we examined 630 unfed i. ricinus and i. persulcatus, ticks collected from four different regions around moscow within an area of 250 km2. eighty-four ticks were culture positive (13.3%) and the prevalence rate varied in each region from 5.7% to 42. ... | 2005 | 15715174 |
| [genetic variants of borrelia afzelii, a pathogen of the ixodes tick borrelioses]. | borrelia afzelii is one of two most important pathogens of the ixodes tick borrelioses (itb) in russia and neighboring countries. this pathogen circulates in various ecosystems and has a wide range of reservoir hosts and transmitters. the results of studies of genetic heterogeneity of the spirochaetae b. afzelii are considered. a total of 139 primary isolates were studied. the isolates were isolated from three species of ixodes ticks at different stages of development and obtained from the labor ... | 2005 | 16173394 |
| susceptibility of borrelia afzelii strains to antimicrobial agents. | the aim of the present study was to determine the susceptibility of borrelia afzelii strains to antibiotics, and to test the hypothesis that persistence of borrelia in skin, after therapy, is a consequence of resistance to the antibiotic used for treatment. ten b. afzelii strains isolated from skin of seven adult patients (two with acrodermatitis chronica atrophicans, five with erythema migrans) were studied. in three patients b. afzelii was isolated from erythema migrans lesion before antibioti ... | 2005 | 15871918 |
| comparison of growth of borrelia afzelii, b. garinii, and b. burgdorferi sensu stricto in mkp and bsk-ii medium. | lyme borreliosis is a tick-borne disease caused by genetically diverse borrelia strains including b. afzelii, b. garinii, and b. burgdorferi sensu stricto (s.s.). the aim of the present study was to assess and compare the growth of one strain per species of b. afzelii, b. garinii, and b. burgdorferi s.s. in modified kelly-pettenkofer (mkp) and barbour-stonner-kelly-ii (bsk-ii) medium, and to check for the presence of the overgrowth after inoculating the media with a mixture of two different borr ... | 2004 | 15595391 |
| [eosinophilic fasciitis associated with borrelia afzelii infection]. | a 58-year-old woman suffered from stiffness, swelling, rubor and muscle pain in the extremities one month after she climbed a mountain in kyushu, an island in southern japan. on admission, mild proximal weakness was present in the extremities. her range of motion in the extremities was limited due to firm skin and subcutaneous stiffness which was similar to scleroderma. she showed peripheral blood eosinophilia and hypergammaglobulinemia as well as a high erythrocyte sedimentation rate. an igm an ... | 2004 | 15287514 |
| effect of tick salivary gland extract on the cytokine production by mouse epidermal cells. | previous studies have demonstrated that both tick saliva and borrelia burgdorferi sensu lato antigens modulate the cytokine response of the host. in this paper, the effect of salivary gland extract (sge) from ixodes ricinus (l., 1758) ticks on cytokine production by primary cultures of mouse epidermal cells stimulated with borrelia afzelii canica, nato, du merle, mazie, baranton et postic, 1993 spirochetes was analysed. epidermal cells were derived from c3h/hen mice, susceptible to lyme disease, ... | 2004 | 15729950 |
| evaluation of groel gene analysis for identification of borrelia burgdorferi sensu lato. | the nucleotide sequences of the groel genes, the flagellin genes, and the 16s rrna genes from 22 reference strains of borrelia were compared. groel sequence analysis is useful not only in interspecies differentiation but also in intraspecies differentiation of borrelia afzelii and borrelia garinii isolates. | 2004 | 15004091 |
| comparison of erythema migrans caused by borrelia afzelii and borrelia garinii. | we compared epidemiological and clinical characteristics of patients with erythema migrans (em) caused by borrelia afzelii and borrelia garinii. | 2004 | 15007737 |
| occurrence of borrelia afzelii and borrelia garinii in ixodes ricinus ticks from southern moravia, czech republic. | during the years 1996-2000, a total of 2398 ixodes ricinus ticks were collected in three areas of southern moravia and eastern bohemia, czech republic, and examined by dark-field microscopy for the presence of spirochetes. the prevalence of b. burgdorferi sensu lato in ixodes ricinus ticks varied and depended upon the year. in 1996, the prevalence 6.8% was observed, during 1997 and 1998, it increased to 8.4% and 12.3%, respectively. the lowest prevalence was observed in 1999 (3.6%), and in 2000 ... | 2004 | 15018772 |
| lyme borreliosis agents and the genetics and sex of their vector, ixodes ricinus. | the tick ixodes ricinus is responsible for the transmission and maintenance of a wide variety of pathogenic organisms in the northern hemisphere, among which lyme disease represents a major threat to humans. despite numerous studies, the epidemiology of the different bacterial species responsible for this disease remains unclear. recent evidence for a sex-biased genetic structure of its european vector leads us to analyse the consequences of this pattern on borrelia transmission. here we show th ... | 2004 | 15026017 |
| adaptation of diverse lyme disease spirochetes in a natural rodent reservoir host. | we compared the relative reservoir competence of european wood mice for two genospecies of lyme disease spirochetes by analyzing susceptibility, intrinsic incubation period, and degree and duration of infectivity. borrelia afzelii, specializing in particular reservoir hosts, is better adapted to those hosts than is the more generalist genospecies b. burgdorferi sensu stricto. | 2004 | 15039378 |
| identification of three clinically relevant borrelia burgdorferi sensu lato genospecies by pcr-restriction fragment length polymorphism analysis of 16s-23s ribosomal dna spacer amplicons. | we report the results of a study of the prevalences of three clinically relevant borrelia burgdorferi sensu lato genospecies (borrelia burgdorferi sensu stricto, borrelia afzelii, and borrelia garinii) in 1,040 questing ixodes ticks from all regions of latvia, where lyme borreliosis is endemic. the prevalences of borrelia in ixodes ricinus and ixodes persulcatus were 22.6 and 27.9%, respectively. molecular typing of b. burgdorferi from infected ticks was performed by restriction fragment length ... | 2004 | 15070987 |
| lysine-dependent multipoint binding of the borrelia burgdorferi virulence factor outer surface protein e to the c terminus of factor h. | serum resistance, an important virulence determinant of borrelia burgdorferi sensu lato strains belonging to the borrelia afzelii and b. burgdorferi sensu stricto genotypes, is related to binding of the complement inhibitor factor h to the spirochete surface protein outer surface protein e (ospe) and its homologues. in this study, we show that the c-terminal short consensus repeats 18-20 of both human and mouse factor h bind to ospe. analogously, factor h-related protein 1, a distinct plasma pro ... | 2004 | 15128807 |
| serological description of estonian patients with lyme disease, a comparison with control sera from endemic and non-endemic areas. | serological tests for lyme disease are mostly not well standardized and cases of misinterpretation of test results by clinicians are rather common. the diagnostic value of serologic tests may also depend on the seroepidemiological situation of the population. the aim of the study was to compare the immunoblot pattern of lyme borreliosis patients and control sera from endemic and non-endemic regions and to identify the most suitable interpretation criteria for our immunoblot test. serum samples o ... | 2004 | 15147004 |
| sequence typing reveals extensive strain diversity of the lyme borreliosis agents borrelia burgdorferi in north america and borrelia afzelii in europe. | the genetic polymorphism of borrelia burgdorferi and borrelia afzelii, two species that cause lyme borreliosis, was estimated by sequence typing of four loci: the rrs-rrla intergenic spacer (igs) and the outer-membrane-protein gene p66 on the chromosome, and the outer-membrane-protein genes ospa and ospc on plasmids. the major sources of dna for pcr amplification and sequencing were samples of the b. burgdorferi tick vector ixodes scapularis, collected at a field site in an endemic region of the ... | 2004 | 15184561 |
| diversity within borrelia burgdorferi sensu lato genospecies in switzerland by reca gene sequence. | a total of 874 ixodes ricinus ticks were collected in switzerland to investigate the genetic diversity of the borrelia population. we integrated to the rt-pcr method the dna sequence analysis of a 162-bp fragment of the reca gene. five genospecies were detected: borrelia afzelii, borrelia burgdorferi s.s., borrelia garinii, borrelia valaisiana, and borrelia lusitaniae. a heterogeneous distribution was observed within the b. burgdorferi s.l. genospecies. the most prevalent and diverse genospecies ... | 2004 | 15336411 |
| molecular characterization of the ospa(161-175) t cell epitope associated with treatment-resistant lyme arthritis: differences among the three pathogenic species of borrelia burgdorferi sensu lato. | treatment-resistant lyme arthritis, which may result from infection-induced autoimmunity, is associated with reactivity to a t cell epitope of outer-surface protein a (ospa(161-175)) of borrelia burgdorferi sensu stricto (bb). this syndrome has been noted primarily in the united states where only bb is present, and rarely in europe where borrelia garinii (bg) and borrelia afzelii (ba) predominate. to gain a better understanding of this epitope, we identified its species-specific polymorphisms, d ... | 2004 | 15501399 |
| prevalence of borrelia burgdorferi sensu lato genospecies in host-seeking ixodes ricinus ticks in selected south bohemian locations (czech republic). | in selected localities of ceské budĕjovice and ceský, krumlov districts, well known by stable high incidence of tick-borne encephalitis (tbe) human cases but with low incidence of lyme borreliosis, monitoring of borrelia burgdorferi sensu lato (s.l.) in ixodes ricinus ticks was performed. research was also aimed at the spread of i. ricinus to mountain areas of this region (national park sumava), as well as at investigating this tick for b. burgdorferi s.l. genospecies and tbe virus infection. al ... | 2004 | 15508414 |
| relationships of a novel lyme disease spirochete, borrelia spielmani sp. nov., with its hosts in central europe. | to determine whether the pathogenic variant of lyme disease spirochetes, isolate a14s, is perpetuated in a particular reservoir-vector relationship, we screened vector ticks in various central european sites for a related spirochete and determined its host association. a14s-like spirochetes infect numerous questing ticks in the petite camargue alsacienne (pc). they frequently infect dormice, but no mice or voles. garden dormice appear to be better reservoir hosts for a14s-like spirochetes than f ... | 2004 | 15528500 |
| terrestrial distribution of the lyme borreliosis agent borrelia burgdorferi sensu lato in east asia. | lyme borreliosis is the most prevalent tick-borne zoonosis and an important emerging infection in europe, north america, and far eastern countries. the geographical distribution of borrelia spp. and the relationship between borrelia spp. and tick spp. in east asian countries have been studied. in northern asian countries, ixodes persulcatus carries eurasian-type borrelia garinii (20047 type), borrelia afzelii, and asian-type b. garinii (variant nt29), whereas borrelia burgdorferi sensu stricto h ... | 2004 | 15623946 |
| improvement in the laboratory recognition of lyme borreliosis with the combination of culture and pcr methods. | lyme disease is a multisystem, multistage infection caused by three genospecies of the borrelia burgdorferi sensu lato species. the diagnosis of lyme disease is based on a history of tick-bite, physical examination, and serological tests. in the seronegative patients with lyme borreliosis symptoms, additional testing should be introduced. | 2003 | 15068385 |
| genetic variability within borrelia burgdorferi sensu lato genospecies established by pcr-single-strand conformation polymorphism analysis of the rrfa-rrlb intergenic spacer in ixodes ricinus ticks from the czech republic. | in europe the borrelia burgdorferi sensu lato complex is represented by five distinct genospecies: borrelia burgdorferi sensu stricto, borrelia afzelii, borrelia garinii, borrelia valaisiana, and borrelia lusitaniae. these taxonomic entities are known to differ in their specific associations with vertebrate hosts and to provoke distinct clinical manifestations in human patients. however, exceptions to these rules have often been observed, indicating that strains belonging to a single genospecies ... | 2003 | 12514035 |
| improved serodiagnosis of erythema migrans using novel recombinant borrelial bbk32 antigens. | the performances of recombinant borrelial bbk32 proteins as antigens in the serology of erythema migrans (em) were evaluated in an elisa. serum samples were obtained from 75 patients from different geographic areas where three borrelial species, borrelia burgdorferi sensu stricto, borrelia afzelii or borrelia garinii, cause lyme borreliosis. antibodies to variant bbk32 proteins were compared with anti-flagella or with anti-ir(6) peptide antibodies. in igg elisa at presentation of em, 65/75 (87 % ... | 2003 | 12808077 |
| [comparative analysis of use of two strains of various genotypes of borrelia burgdorferi sensu lato as antigens for antibody identification in ixodes tick borreliosis by indirect immunofluorescence]. | the results of the indirect reaction of immune-fluorescence (irif) was studied in testing 49 sera of 19 patients with lyme-borreliosis with antigens of genotypes borrelia afzeli (strain jp-21) and borrelia burgdorferi sensu stricto (strain no. 17); rabbit fluorescini-sothiocyanate-marked conjugates to human immunoglobulins m and g as well as polyvalent conjugate were used of. no reliable differences were found between all positive and all negative results. the biggest portion of positive results ... | 2003 | 12822309 |
| genetic diversity of bacterial agents detected in ticks removed from asymptomatic patients in northeastern italy. | a total of 360 ticks were removed from 353 asymptomatic subjects in belluno province, italy and surrounding areas, from 1998 to 2001. ticks were identified as ixodes ricinus (357), ixodes hexagonus (1), rhipicephalus sanguineus (1), and ixodes ventalloi (1). tick dna was investigated by pcr and subsequent sequencing of amplified products to identity associated bacterial agents. primers targeting different genes of rickettsia (glta and ompa), borrelia (16s rdna, rpob), francisella (16s rdna), and ... | 2003 | 12860623 |
| co-feeding transmission and its contribution to the perpetuation of the lyme disease spirochete borrelia afzelii. | 2003 | 12899145 | |
| lyme borreliosis. | lyme borreliosis is a multi-organ infection caused by spirochetes of the borrelia burgdorferi sensu lato group with its species b burgdorferi sensu stricto, borrelia garinii, and borrelia afzelii, which are transmitted by ticks of the species ixodes. laboratory testing of lyme borreliosis includes culture, antibody detection using elisa with whole extracts or recombinant chimeric borrelia proteins, immunoblot, and pcr with different levels of sensitivity and specificity for each test. common ski ... | 2003 | 12901891 |
| first isolation and characterization of borrelia burgdorferi sensu lato strains from ixodes ricinus ticks in turkey. | in order to investigate the presence and prevalence of lyme borreliosis (lyme disease) borrelia species, 312 unfed ticks were collected by flagging at a woodland area in trakya, in the european side of turkey, in may 2002. twelve of 299 ixodes ricinus ticks were infected with borrelia spp., as determined by cultivation in bsk medium (prevalence rate 4.0 %). ten pure cultures were subjected to further characterization by sequencing analysis of the 5s-23s rdna intergenic spacer, 16s rdna and flage ... | 2003 | 12909659 |
| identification of borrelia burgdorferi sensu lato, anaplasma and ehrlichia species, and spotted fever group rickettsiae in ticks from southeastern europe. | prevalence data for tick-borne pathogens are used to assess the risk for human health. in this study the presence and identity of borrelia burgdorferi sensu lato, ehrlichia, anaplasma, and rickettsia species in bulgarian ixodes ricinus ticks and in non- ixodes ticks from turkey and albania was determined by polymerase chain reaction (pcr) and reverse line blot hybridization. in the adult bulgarian ticks, the prevalence of borrelia burgdorferi sensu lato infection was approximately 40%, while bor ... | 2003 | 12938010 |
| prevalence of borreliae in ixodid ticks from a floodplain forest ecosystem. | host-seeking ixodid ticks were sampled in a floodplain forest ecosystem along the lower reaches of the thaya (dyje) river in south moravia (czech republic) and lower austria during the period 1989-2002. the ticks were examined by dark-field microscopy for borreliae (borrelia burgdorferi sensu lato, the agent of lyme borreliosis), and attempts were made to culture the spirochetes in bsk-h medium from preparations containing their high numbers. isolated borreliae were identified by pcr-rflp analys ... | 2003 | 12674689 |
| in vitro susceptibility testing of four antibiotics against borrelia burgdorferi: a comparison of results for the three genospecies borrelia afzelii, borrelia garinii, and borrelia burgdorferi sensu stricto. | mics and minimal bactericidal concentrations (mbcs) were evaluated for the four antibiotics azithromycin, amoxicillin, ceftriaxone, and doxycycline against the three main genospecies of borrelia burgdorferi sensu lato. in mbc testing, statistically significant differences between the genospecies could be found in 7 out of 12 comparative evaluations (p < 0.05). | 2003 | 12682190 |
| characteristics of erythema migrans in borrelia afzelii and borrelia garinii infections. | erythema migrans is the most common manifestation of stage i lyme borreliosis. this study investigated the relation between the subspecies of borrelia burgdorferi involved, the time of development and the appearance of the manifestation. the study comprised 33 cases of erythema migrans yielding b. burgdorferi sensu lato by culture of skin biopsy. the species was determined by polymerase chain reaction analysis of the cultivated spirochaetes. in 22 of the cases, b. afzelii was demonstrated, where ... | 2003 | 12685881 |
| prevalence of granulocytic ehrlichia and borrelia burgdorferi sensu lato in ixodes ricinus ticks collected from southwestern finland and from vormsi island in estonia. | altogether, 343 adult and 111 nymphal ixodes ricinus ticks collected from parks in turku and suburban and rural islands of the turku archipelago, finland, and 100 adult i. ricinus ticks collected from vormsi island, estonia, were included in this study. using the polymerase chain reaction the ticks were examined for 16s rdna of the ehrlichia phagocytophila genogroup and for borrelia burgdorferi sensu lato reca and flagellin genes. none of the finnish ticks was found to be infected with e. phagoc ... | 2003 | 12716393 |
| prevalence of four species of borrelia burgdorferi sensu lato and coinfection with anaplasma phagocytophila in ixodes ricinus ticks in central germany. | a total of 305 ixodes ricinus ticks collected from three areas of thuringia in central germany were investigated for infection with borrelia burgdorferi sensu lato species and anaplasma phagocytophila. overall, 11.1% were infected with borrelia burgdorferi and 2.3% with anaplasma phagocytophila. adult ticks showed a significantly higher rate of infection with both borreliae and anaplasma phagocytophila. borrelia garinii (55.9%) was detected most frequently, followed by borrelia burgdorferi sensu ... | 2003 | 12783276 |
| prevalence of borrelia burgdorferi sensu lato genomospecies and of the human granulocytic ehrlichiosis (hge) agent in ixodes ricinus ticks collected in the area of monti lepini, italy. | ticks are obligate hematophagous arthropods that are parasites in every class of vertebrates in most regions of the world. they are also considered to be important vectors for the transmission of human infectious diseases. in the present study we used polymer chain reaction (pcr) amplification analysis to determine the prevalence of borrelia burgdorferi and ehrlichia phagocytophila, the agents of, respectively, lyme borreliosis and human granulocytic ehrlichiosis, among ticks inhabiting the area ... | 2003 | 12797900 |
| detection and identification of pathogens and host dna in unfed host-seeking ixodes ricinus l. (acari: ixodidae). | in this study, we have developed molecular methods for the identification of reservoir hosts of sylvatic tick-borne zoonoses. the methods are based on the analysis of the blood meal remnant in the tick gut and include detection of pathogens and identification of the host origin of the blood meal. for host identification, a universal primer pair was used to amplify part of the vertebrate 18s rrna gene followed by reverse line blot hybridization using subgroup-specific probes. analyses of dna from ... | 2003 | 14596289 |
| genetic diversity among borrelia strains determined by single-strand conformation polymorphism analysis of the ospc gene and its association with invasiveness. | lyme borreliosis (lb) is a tick-borne spirochetal infection caused by three borrelia species: borrelia afzelii, b. garinii, and b. burgdorferi sensu stricto. lb evolves in two stages: a skin lesion called erythema migrans and later, different disseminated forms (articular, neurological, cardiac.). previous research based on analysis of ospc sequences allowed the definition of 58 groups (divergence of <2% within a group and >8% between groups). only 10 of these groups include all of the strains i ... | 2003 | 14605139 |
| improved serodiagnosis of early lyme borreliosis: immunoblot with local borrelia afzelii strain. | to improve the serodiagnosis of lyme borreliosis (lb) the performances of four tests were evaluated. an indirect immunofluorescent assay based on borrelia burgdorferi s.s., enzyme-linked immunosorbent assay (elisa) based on local isolates of borrelia afzelii and b. burgdorferi s.s., and immunoblot (ib) of b. afzelii were prepared. the serum panels contained 214 serum samples: control group (n=120) and patients at different stages of lb (n=94). the specificity of ib was 96%, of in-house elisa 93% ... | 2003 | 14629271 |
| evidence of babesia microti infection in multi-infected ixodes persulcatus ticks in russia. | to detect babesia-infected ixodes persulcatus shulze in a suburb of st. petersburg, russia, 738 adult ticks were studied using babesia specific primers and pcr techniques. the entire sample (more than 1,200 individuals) was screened for the presence of borrelia spp., ehrlichia spp. and tick-borne encephalitis virus (tbev). all 7 ticks infected with babesia microti, were also infected with other pathogens (all 7 among 417 infected ticks, zero amongst the remaining 321 naive ones (chi2 = 5.25, p<0 ... | 2003 | 14635819 |
| solitary and multiple erythema migrans in children: comparison of demographic, clinical and laboratory findings. | data on european children with erythema migrans (em) are limited. | 2003 | 14735383 |
| dynamics of cytokine production in human peripheral blood mononuclear cells stimulated by lps or infected by borrelia. | to specify the role of individual cytokines in the immune response to pyrogens, isolated and cultivated human peripheral blood mononuclear cells (pbmc) were used for the experiments. different pyrogens (lipopolysaccharide from escherichia coli - lps and live borrelia afzelii) were applied and the time course of changes in concentrations of different cytokines in the medium was followed using the elisa method. it was found that nonstimulated human pbmc proliferate under in vitro conditions and pr ... | 2003 | 14964289 |
| dynamics of cytokine production in human peripheral blood mononuclear cells stimulated by lps or infected by borrelia. | to specify the role of individual cytokines in the immune response to pyrogens, isolated and cultivated human peripheral blood mononuclear cells (pbmc) were used for the experiments. different pyrogens (lipopolysaccharide from escherichia coli - lps and live borrelia afzelii) were applied and the time course of changes in concentrations of different cytokines in the medium was followed using the elisa method. it was found that nonstimulated human pbmc proliferate under in vitro conditions and pr ... | 2003 | 14535835 |
| recombinant or peptide antigens in the serology of lyme arthritis in children. | the performance of elisas with the recombinant antigens decorin-binding protein a (dbpa), dbpb, and bbk32 (from borrelia afzelii, b. garinii, and b. burgdorferi sensu stricto) and vlse peptide antigen invariable region 6 (ir(6)) were evaluated in the serodiagnosis and follow-up of children with lyme arthritis (la). serum samples were obtained from 52 children with clinically typical and serologically confirmed la. in igg elisas, at diagnosis, 50 samples were positive for bbk32, 51 for dbpa, 40 f ... | 2003 | 12792865 |
| borrelia afzelii evidenced by polymerase chain reaction in a biopsy of nipple lymphocytoma. | 2003 | 12588408 | |
| characterization of the vls antigenic variation loci of the lyme disease spirochaetes borrelia garinii ip90 and borrelia afzelii acai. | the vls locus of borrelia burgdorferi b31 consists of 15 silent cassettes and one expression site (vlse), and the presence of the encoding plasmid lp28-1 correlates with high infectivity. recombination between the expression cassette and silent cassettes occurs in vivo, and this process may enable b. burgdorferi to evade the immune response. to determine the characteristics of the vls loci in other borrelia strains, we have cloned and characterized the vls silent cassette loci of borrelia garini ... | 2003 | 12603744 |
| association of borrelia afzelii with rodents in europe. | borrelia burgdorferi sensu lato (s.l.) is maintained in nature by complex zoonotic transmission cycles, involving a large variety of vertebrates as hosts and hard ticks of the genus ixodes as vectors. recent studies suggest that the genospecies of b. burgdorferi s.l. and sometimes their subtypes are propagated by different spectra of hosts, mainly birds and rodents. in order to test the concept of host-association, we analysed the relationships between borrelia genospecies, rodent hosts and i. r ... | 2003 | 12613759 |
| prevalence of borrelia burgdorferi species and identification of borrelia valaisiana in questing ixodes ricinus in the lyon region of france as determined by polymerase chain reaction-restriction fragment length polymorphism. | many cases of lyme borreliosis have been reported over the years in the region of lyon, france. the identification and prevalence of borrelia burgdorferi sensu lato in ixodes ricinus were investigated by polymerase chain reaction (pcr) of the flagellin gene and restriction fragment length polymorphism (rflp) analysis. questing ixodes ricinus larvae, nymphs and adults were collected by the flagging method from deciduous forests in four areas in the lyon region of france between october 1994 and s ... | 2003 | 12649714 |
| investigations on the mode and dynamics of transmission and infectivity of borrelia burgdorferi sensu stricto and borrelia afzelii in ixodes ricinus ticks. | borrelia burgdorferi sensu lato (sl), the agent of lyme disease, is transmitted to the host during the blood meal of ixodes ticks. in most unfed ticks, spirochetes are present in the midgut and migrate during blood feeding to the salivary glands, from which they are transmitted to the host via saliva. in the present study, the efficiency of ixodes ricinus ticks to transmit b. afzelii and b. burgdorferi sensu stricto (ss) and their infectivity for mice were examined in relation to the duration of ... | 2002 | 12656125 |
| distribution of clinically relevant borrelia genospecies in ticks assessed by a novel, single-run, real-time pcr. | a lightcycler-based pcr protocol was developed which targets the ospa gene for the identification and quantification of the different borrelia burgdorferi sensu lato species in culture and in ticks, based on the use of a fluorescently labeled probe (hybprobe) and an internally labeled primer. the detection limit of the pcr was 1 to 10 spirochetes. a melting temperature determined from the melting curve of the amplified product immediately after thermal cycling allowed the differentiation of the ... | 2002 | 11773090 |
| experimental infection of laboratory mice and rabbits with several isolates of borrelia burgdorferi sensu lato; comparison of antigens from different genospecies in serological measurement of immune responses. | infectivity of and immune responses to 28 finnish borrelia burgdorferi sensu lato isolates was studied in 3-4-week-old outbred nmri and inbred balb/c/hy laboratory mice; rabbits were also inoculated. twenty-one isolates were found to detectably infect mice. a variation among isolates in degree of infectivity was observed. higher infection rate and higher average elisa readings were recorded for intradermal than intraperitoneal inoculations. the results suggest differences between borrelia genosp ... | 2002 | 11848128 |
| lyme disease and current aspects of immunization. | lyme disease is a tick-borne multisystem disease that affects primarily the skin, nervous system, heart and joints. at least three species of borrelia burgdorferi sensu lato, namely borrelia burgdorferi sensu stricto, borrelia garinii, and borrelia afzelii, can cause the disease. this review will focus mainly on the pathophysiology of lyme arthritis, the long-term outcome of lyme disease, and the recently licensed vaccine against lyme disease. | 2002 | 11879534 |
| dynamics of dissemination and outer surface protein expression of different european borrelia burgdorferi sensu lato strains in artificially infected ixodes ricinus nymphs. | unfed ixodes ricinus nymphs were infected with eight different strains and clones of borrelia afzelii and b. garinii by capillary feeding. except one b. afzelii clone, all expressed ospc in culture. tick midguts and salivary glands were investigated at different time intervals for the presence of borreliae and for ospa and ospc phenotypes by immunofluorescence with simultaneous staining of ospa and ospc with monoclonal antibodies. both species were transmittable to i. ricinus. all ospc-expressin ... | 2002 | 11923373 |
| [borrelia strains that cause erythema migrans in alsace, france]. | in europe, borrelia burgdorferi sensu stricto, borrelia afzelii and borrelia garinii are known as pathogens among the borrelia burgdorferi sensu lato group. since it is not yet known which borrelia are responsible for lyme borreliosis in france, the objective of this study was to identify the species of borrelia responsible for erythema migrans in the region of alsace, france. | 2002 | 11937923 |
| [lyme borreliosis--incidence of serum anti-myelin antibodies]. | the method of enzyme immunoassay (elisa) was used for detection of antibodies against the basic protein myelin (antimyelin antibodies) for a group of serum samples (n 36) with positive anti-borrelia immunoglobulins igg and igm (elisa-borrelia afzelii) and their immune complexes (elisa-peg). antimyelin antibodies (elisa-doxa kit-myelin basic protein antibodies) were assessed in 31% (n 11) of examined serum samples of patients with the working diagnosis of lyme borreliosis. statistical analysis (p ... | 2002 | 11987581 |
| dna microarray assessment of putative borrelia burgdorferi lipoprotein genes. | a dna microarray containing fragments of 137 borrelia burgdorferi b31 putative lipoprotein genes was used to examine lyme disease spirochetes. dna from b. burgdorferi sensu stricto b31, 297, and n40; borrelia garinii ip90; and borrelia afzelii p/gau was fluorescently labeled and hybridized to the microarray, demonstrating the degree to which the individual putative lipoprotein genes were conserved among the genospecies. these data show that a dna microarray can globally examine the genes encodin ... | 2002 | 12011030 |
| three major lyme borrelia genospecies (borrelia burgdorferi sensu stricto, b. afzelii and b. garinii) identified by pcr in cerebrospinal fluid from patients with neuroborreliosis in sweden. | the lyme borrelia genospecies borrelia afzelii and b. garinii have previously been isolated using a culture method in swedish patients with lyme borreliosis (lb). there are reports suggesting that the genospecies distribution in human tissue specimens as determined by molecular methods is different from that obtained by culture. in the present study, we developed a nested pcr for detection of lyme borrelia-specific dna in cerebrospinal fluid from swedish patients with lb. the genospecies were su ... | 2002 | 12069016 |
| epitope mapping of the outer surface protein a (ospa) of the spirochete borrelia burgdorferi using a panel of monoclonal antibodies and lanthanide competition fluoroimmunoassay. | a panel of fourteen different monoclonal antibodies was used for detection and analysis of antigenic determinants located on the outer surface protein a (ospa) of the spirochete borrelia burgdorferi, which is a causative agent of tick-borne borreliosis (lyme disease). two main and several minor partially overlapping antigenic determinants have been found on the surface of the ospa protein of borrelia burgdorferi sensu stricto (strain 297) by lanthanide competition fluoroimmunoassay. one of the m ... | 2002 | 12126471 |
| horses and borrelia: immunoblot patterns with five borrelia burgdorferi sensu lato strains and sera from horses of various stud farms in austria and from the spanish riding school in vienna. | grazing animals are continuously exposed to tick bites. consequently, one may expect that horses will become infected with the various pathogens carried by ticks including borrelia burgdorferi sensu lato. whether horses may develop clinical disease due to this pathogen is controversially discussed. we were interested to learn about the infection of horses with borrelia burgdorferi sensu lato within one season by studying the dynamics of the humoral immune response in paired blood samples. the ma ... | 2002 | 12141766 |
| tick salivary gland extract-activated transmission of borrelia afzelii spirochaetes. | saliva-activated transmission of borrelia afzelii canica, nato, du merle, mazie, baranton et postic, 1993 was demonstrated using salivary gland extract (sge) from ixodes ricinus (l., 1758) ticks and c3h mice. injection of borrelia spirochaetes together with sge increased the level of bacteraemia and accelerated the appearance of bacteria in the urinary bladder, compared with the injection of spirochaetes alone. more i. ricinus nymphs became infected when feeding on mice inoculated with b. afzeli ... | 2002 | 12194488 |
| nodular fasciitis, erythema migrans, and oligoarthritis: manifestations of lyme borreliosis caused by borrelia afzelii. | we describe a 35-year old patient with nodular fasciitis, erythema migrans, and gonarthritis four months after a bite of a borrelia afzelii infected tick. the borrelia afzelii infection was identified by a polymerase chain reaction and direct sequencing of the amplification product. borrelia-specific dna was also detectable in nodular fasciitis tissue. we therefore conclude that borrelia afzelii can be a causative agent of nodular fasciitis and lyme arthritis in a highly endemic region of northe ... | 2002 | 12195636 |
| [use of western-immunoblotting to determine serum igg-antibodies to antigens of different genotypes of borrelia--borrelia burgdorferi sensu stricto, borrelia afzelii, and borrelia garinii]. | the reaction of the sera from 86 patients with lyme borreliosis was evaluated in the immunoblotting using three genotypes of pathogenic borrelia strains. the russian isolates of borrelia afzelii (strain ip3) and b. garinii 20047t (strain ip90) were compared with the usa typical strain b31--b. burgdorferi sensu stricto. the results were assessed by the criteria recommended for the usa and developed for b. burgdorferi sensu stricto. certain differences were shown in the reactions of serum igg with ... | 2002 | 12214528 |